Peter J Parker

Summary

Affiliation: Cancer Research UK
Country: UK

Publications

  1. ncbi request reprint PKC at a glance
    Peter J Parker
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    J Cell Sci 117:131-2. 2004
  2. ncbi request reprint Detecting protein-phospholipid interactions. Epidermal growth factor-induced activation of phospholipase D1b in situ
    William E Hughes
    Protein Phosphorylation Laboratory, Cancer Research United Kingdom London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 277:22974-9. 2002
  3. pmc Receptor trafficking controls weak signal delivery: a strategy used by c-Met for STAT3 nuclear accumulation
    Stephanie Kermorgant
    Department of Tumour Biology, Cancer Research UK Clinical Centre, Bart s and the London Queen Mary s School of Medicine and Dentistry, London EC1M 6BQ, England, UK
    J Cell Biol 182:855-63. 2008
  4. doi request reprint Regulation of the tumour suppressor Fbw7α by PKC-dependent phosphorylation and cancer-associated mutations
    Joanne Durgan
    London Research Institute, Cancer Research UK, UK
    Biochem J 432:77-87. 2010
  5. doi request reprint A cancer-associated mutation in atypical protein kinase Cι occurs in a substrate-specific recruitment motif
    Mark Linch
    1Protein Phosphorylation Laboratory, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Sci Signal 6:ra82. 2013
  6. doi request reprint ERK2 but not ERK1 mediates HGF-induced motility in non-small cell lung carcinoma cell lines
    Simone Radtke
    London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    J Cell Sci 126:2381-91. 2013
  7. pmc An aPKC-exocyst complex controls paxillin phosphorylation and migration through localised JNK1 activation
    Carine Rosse
    Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, London, United Kingdom
    PLoS Biol 7:e1000235. 2009
  8. doi request reprint The identification and characterization of novel PKCepsilon phosphorylation sites provide evidence for functional cross-talk within the PKC superfamily
    Joanne Durgan
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem J 411:319-31. 2008
  9. doi request reprint Adenosine-binding motif mimicry and cellular effects of a thieno[2,3-d]pyrimidine-based chemical inhibitor of atypical protein kinase C isoenzymes
    Svend Kjær
    Structural Biology, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Biochem J 451:329-42. 2013
  10. doi request reprint The regulated assembly of a PKCepsilon complex controls the completion of cytokinesis
    Adrian T Saurin
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, London, WC2A 3PX, UK
    Nat Cell Biol 10:891-901. 2008

Detail Information

Publications66

  1. ncbi request reprint PKC at a glance
    Peter J Parker
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    J Cell Sci 117:131-2. 2004
  2. ncbi request reprint Detecting protein-phospholipid interactions. Epidermal growth factor-induced activation of phospholipase D1b in situ
    William E Hughes
    Protein Phosphorylation Laboratory, Cancer Research United Kingdom London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 277:22974-9. 2002
    ..Application of this approach will facilitate the spatial resolution of many protein-phospholipid interactions that are key events in the regulation of cellular processes...
  3. pmc Receptor trafficking controls weak signal delivery: a strategy used by c-Met for STAT3 nuclear accumulation
    Stephanie Kermorgant
    Department of Tumour Biology, Cancer Research UK Clinical Centre, Bart s and the London Queen Mary s School of Medicine and Dentistry, London EC1M 6BQ, England, UK
    J Cell Biol 182:855-63. 2008
    ..This response is triggered from peripheral endosomes. Thus, control of growth factor receptor traffic determines the nature of the signal output, providing novel opportunities for intervention...
  4. doi request reprint Regulation of the tumour suppressor Fbw7α by PKC-dependent phosphorylation and cancer-associated mutations
    Joanne Durgan
    London Research Institute, Cancer Research UK, UK
    Biochem J 432:77-87. 2010
    ..Together these results provide evidence for both physical and functional interactions between the PKC and Fbw7 families, and yield insights into the isoform-specific regulation of Fbw7α...
  5. doi request reprint A cancer-associated mutation in atypical protein kinase Cι occurs in a substrate-specific recruitment motif
    Mark Linch
    1Protein Phosphorylation Laboratory, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Sci Signal 6:ra82. 2013
    ..Thus, the R471C substitution is a change-of-function mutation acting at this substrate-specific recruitment site to selectively disrupt the polarizing activity of PKCι...
  6. doi request reprint ERK2 but not ERK1 mediates HGF-induced motility in non-small cell lung carcinoma cell lines
    Simone Radtke
    London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    J Cell Sci 126:2381-91. 2013
    ....
  7. pmc An aPKC-exocyst complex controls paxillin phosphorylation and migration through localised JNK1 activation
    Carine Rosse
    Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, London, United Kingdom
    PLoS Biol 7:e1000235. 2009
    ....
  8. doi request reprint The identification and characterization of novel PKCepsilon phosphorylation sites provide evidence for functional cross-talk within the PKC superfamily
    Joanne Durgan
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem J 411:319-31. 2008
    ..Thus our current findings identify three new phosphorylation sites that contribute to the isoform-specific function of PKCepsilon and highlight a novel and direct means of cross-talk between different members of the PKC superfamily...
  9. doi request reprint Adenosine-binding motif mimicry and cellular effects of a thieno[2,3-d]pyrimidine-based chemical inhibitor of atypical protein kinase C isoenzymes
    Svend Kjær
    Structural Biology, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Biochem J 451:329-42. 2013
    ..We conclude that this compound can be used as a chemical tool to modulate aPKC activity in vitro and in vivo and may guide the search for further aPKC-selective inhibitors...
  10. doi request reprint The regulated assembly of a PKCepsilon complex controls the completion of cytokinesis
    Adrian T Saurin
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, London, WC2A 3PX, UK
    Nat Cell Biol 10:891-901. 2008
    ..This study therefore identifies a new regulatory mechanism that controls exit from cytokinesis, which has implications for carcinogenesis...
  11. doi request reprint Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration
    Katrina Boeckeler
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, London, WC2A 3PX, UK
    J Cell Sci 123:2725-32. 2010
    ..The data further show that restored focal adhesion dynamics are a contributing mechanism through which localized ERK activity influences this aPKC-exocyst-dependent migration...
  12. doi request reprint Binding of dynein intermediate chain 2 to paxillin controls focal adhesion dynamics and migration
    Carine Rosse
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, London WC2A 3PX, UK
    J Cell Sci 125:3733-8. 2012
    ..This interaction is controlled by serine 84 phosphorylation, which lies on the aPKC pathway. The evidence presented thus links aPKC control of migration to the dynein control of FA turnover through paxillin...
  13. pmc HER2 oncogenic function escapes EGFR tyrosine kinase inhibitors via activation of alternative HER receptors in breast cancer cells
    Anthony Kong
    Cell Biophysics Laboratory, Lincoln s Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom
    PLoS ONE 3:e2881. 2008
    ..Furthermore, although TKIs suppress the growth of HER2-overexpressing breast tumor cells, they do not fully inhibit HER2 oncogenic function at physiological doses...
  14. ncbi request reprint PKC alpha protein but not kinase activity is critical for glioma cell proliferation and survival
    Angus J Cameron
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, WC2A 3PX London, United Kingdom
    Int J Cancer 123:769-79. 2008
    ..These results indicate an essential pro-proliferative and pro-survival role for PKCalpha in glioma but question the use of ATP competitive inhibitors as therapeutics, either alone, or in combination with chemotoxic agents...
  15. pmc 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins
    Philippe Riou
    Randall Division of Cell and Molecular Biophysics, New Hunt s House, King s College London, London, UK
    Cell 153:640-53. 2013
    ..In contrast to the canonical GTP/GDP switch that regulates most Ras superfamily members, our results reveal an unprecedented mechanism for G protein inhibition by 14-3-3 proteins...
  16. ncbi request reprint Novel phosphorylation site markers of protein kinase C delta activation
    Joanne Durgan
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London, UK
    FEBS Lett 581:3377-81. 2007
    ..These data indicate that PKCdelta is phosphorylated upon activation and that phospho-S299 represents a useful marker of the activated enzyme...
  17. doi request reprint PKC and the control of localized signal dynamics
    Carine Rosse
    Protein Phosphorylation Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Rev Mol Cell Biol 11:103-12. 2010
    ..Examples of where and how various PKC isoforms direct this tier of signal organization are becoming more evident...
  18. pmc Atypical protein kinase Cι as a human oncogene and therapeutic target
    Peter J Parker
    London Research Institute, Lincoln s Inn Fields, London WC2A 3LY, UK King s College London, Guy s Campus, London, UK
    Biochem Pharmacol 88:1-11. 2014
    ..In this review, we discuss the role of PKCiota in transformation and describe mechanism-based approaches to therapeutically target oncogenic PKCiota signaling in cancer. ..
  19. pmc Phosphorylation is required for PMA- and cell-cycle-induced degradation of protein kinase Cdelta
    Jyoti Srivastava
    Protein Phosphorylation Laboratory, Cancer Research UK London Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, U K
    Biochem J 368:349-55. 2002
    ..Analysis of phosphorylation-site mutants indicated that the T-loop Thr(505) phosphorylation site was critical for induced degradation...
  20. ncbi request reprint PKCzetaII is a target for degradation through the tumour suppressor protein pVHL
    Xavier Iturrioz
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields Laboratories, London WC2A 3PX, UK
    FEBS Lett 581:1397-402. 2007
    ..The results indicate that pVHL recruits PKCzetaII via its PB1 domain and causes ubiquitination and degradation via the distal C-terminus of PKCzetaII...
  21. pmc Intramolecular and intermolecular interactions of protein kinase B define its activation in vivo
    Véronique Calleja
    Cell Biophysics Laboratory, Lincoln s Inn Fields Laboratories, London Research Institute, Cancer Research UK, London, United Kingdom
    PLoS Biol 5:e95. 2007
    ..This has important implications not only in extending our understanding of this oncogenic protein kinase but also in opening up distinct opportunities for therapeutic intervention...
  22. pmc Recognition of an intra-chain tandem 14-3-3 binding site within PKCepsilon
    Brenda Kostelecky
    Structural Biology Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    EMBO Rep 10:983-9. 2009
    ..This dual-site intra-chain recognition has implications for other 14-3-3 targets, which seem to have only a single 14-3-3 motif, as other lower affinity and cryptic 14-3-3 gatekeeper sites might exist...
  23. doi request reprint Anomalous inhibition of c-Met by the kinesin inhibitor aurintricarboxylic acid
    Mina Milanovic
    London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3LY, UK
    Int J Cancer 130:1060-70. 2012
    ..Cancer Res 2010;70:1625-34), our findings suggest that novel anti-c-Met therapies could be developed in the future for cancer treatment...
  24. pmc PKCα and PKCδ regulate ADAM17-mediated ectodomain shedding of heparin binding-EGF through separate pathways
    Marie Kveiborg
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, London, United Kingdom
    PLoS ONE 6:e17168. 2011
    ..Together, these findings indicate the existence of multiple modes of regulation controlling EGFR-ligand availability and subsequent EGFR signal transduction...
  25. doi request reprint Functional implications of assigned, assumed and assembled PKC structures
    Mark Linch
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, U K
    Biochem Soc Trans 42:35-41. 2014
    ..These principles have an impact in unexpected ways, from the regulation of membrane targeting, through strategies for pharmacological intervention, to biomarkers. ..
  26. doi request reprint The scaffold MyD88 acts to couple protein kinase Cepsilon to Toll-like receptors
    Amir Faisal
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, London WC2A 3PX, United Kingdom
    J Biol Chem 283:18591-600. 2008
    ..This study therefore identifies the scaffold protein MyD88 as the link coupling TLRs to PKCepsilon recruitment, phosphorylation, and downstream signaling...
  27. doi request reprint mTORC2 targets AGC kinases through Sin1-dependent recruitment
    Angus J M Cameron
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Biochem J 439:287-97. 2011
    ..This inducible selective mTORC2 intervention is used to demonstrate a key role for mTORC2 in cell proliferation in three-dimensional culture...
  28. doi request reprint Protein kinase C intervention: the state of play
    Jon Roffey
    Discovery Laboratory, Cancer Research Technology Limited, Wolfson Institute for Biomedical Research, London, UK
    Curr Opin Cell Biol 21:268-79. 2009
    ..The promise of isoform selective inhibitors and the rationale for their development are discussed in the broader context of the PKC inhibitor arsenal...
  29. pmc Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration
    Sylvie Lachmann
    Protein Phosphorylation Laboratory, London Research Institute, London, United Kingdom
    PLoS ONE 6:e21732. 2011
    ..It is concluded that intervention in PKNs may need to be directed at multiple isoforms to be effective in different cell types...
  30. doi request reprint Site recognition and substrate screens for PKN family proteins
    Alejandra Collazos
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem J 438:535-43. 2011
    ..This identified Thr⁶⁵⁴ in EGFR as the PKN1 phosphorylation site and this retains an arginine residue at the -3 position. Finally, the constitutive phosphorylation of EGFR on Thr⁶⁵⁴ is shown to be modulated by PKN in vivo...
  31. doi request reprint PKC maturation is promoted by nucleotide pocket occupation independently of intrinsic kinase activity
    Angus J M Cameron
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, London, UK
    Nat Struct Mol Biol 16:624-30. 2009
    ..These data demonstrate that autophosphorylation is not required for PKC priming and show how ATP pocket occupation can enable a kinase to mature as well as function...
  32. pmc The von Hippel-Lindau tumour-suppressor protein interaction with protein kinase Cdelta
    Xavier Iturrioz
    Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Biochem J 397:109-20. 2006
    ..Thus, in contrast with aPKC, PKCdelta is not a conventional substrate of the ubiquitin-ligase complex, VCB-Cul-2, and the observed interaction between these two proteins must underlie a distinct signalling output...
  33. pmc A selective PIKfyve inhibitor blocks PtdIns(3,5)P(2) production and disrupts endomembrane transport and retroviral budding
    Harold B J Jefferies
    London Research Institute Cancer Research UK, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    EMBO Rep 9:164-70. 2008
    ..We concluded that the phosphatidylinositol 3,5-bisphosphate pathway is integral to endosome formation, determining morphology and cargo flux...
  34. ncbi request reprint Protein kinase C epsilon in cell division: control of abscission
    Adrian T Saurin
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, London, UK
    Cell Cycle 8:549-55. 2009
    ..Here we will discuss new findings that implicate Protein Kinase C epsilon (PKCepsilon) as a regulator of RhoA signalling required for abscission...
  35. doi request reprint Localised interventions in cellular processes
    Nick Peel
    Protein Phosphorylation Laboratory London Research Institute, London, UK
    Biochim Biophys Acta 1834:1364-70. 2013
    ..This article is part of a Special Issue entitled: Inhibitors of Protein Kinases (2012)...
  36. ncbi request reprint PKCepsilon is a permissive link in integrin-dependent IFN-gamma signalling that facilitates JAK phosphorylation of STAT1
    Johanna Ivaska
    Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Cell Biol 5:363-9. 2003
    ..Thus, PKCepsilon functions as a central point of integration through which integrin engagement exerts a permissive input on IFN-gamma signalling...
  37. ncbi request reprint Hyperosmotic-induced protein kinase N 1 activation in a vesicular compartment is dependent upon Rac1 and 3-phosphoinositide-dependent kinase 1
    Neil E Torbett
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 278:32344-51. 2003
    ..Taken together, our findings present a pathway for the selective hyperosmotic-induced Rac1-dependent PKN1 translocation and PDK1-dependent activation...
  38. doi request reprint Pseudokinase drug intervention: a potentially poisoned chalice
    Jeroen Claus
    Cancer Research UK, London Research Institute, Lincoln s Inn Fields, London WC2A 3LY, UK
    Biochem Soc Trans 41:1083-8. 2013
    ..We discuss the implications of locking particular conformations in a selection of (pseudo)kinases and the dual potential impact on the druggability of these proteins. ..
  39. ncbi request reprint Fab1p and AP-1 are required for trafficking of endogenously ubiquitylated cargoes to the vacuole lumen in S. cerevisiae
    John P Phelan
    Department of Biochemistry and Molecular Biology, University College London, Darwin Building, Gower Street, London, WC1E 6BT, UK
    J Cell Sci 119:4225-34. 2006
    ..Our data imply that AP-1 is required for some Fab1p and PtdIns(3,5)P2-dependent processes...
  40. pmc Identification of PKCzetaII: an endogenous inhibitor of cell polarity
    Scott J Parkinson
    Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, London, UK
    EMBO J 23:77-88. 2004
    ..The data demonstrate a regulatory role for PKCzetaII in the maintenance of cell transformation and the development of cell polarity...
  41. ncbi request reprint Protein kinase C phosphorylation: an introduction
    Peter J Parker
    Cancer Research UK, London Research Institute, UK
    Methods Mol Biol 233:159-62. 2003
  42. pmc FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2
    Olivier E Pardo
    Lung Cancer Biology Group, Cancer Research UK, Imperial College London, Hammersmith Hospitals Campus, Du Cane Road, London, UK
    EMBO J 25:3078-88. 2006
    ..However, increased S6K1 kinase activity has no such effect. Thus, S6K2 but not S6K1 mediates prosurvival/chemoresistance signalling...
  43. ncbi request reprint Dephosphorylation of PKCdelta by protein phosphatase 2Ac and its inhibition by nucleotides
    Jyoti Srivastava
    Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, WC2A 3PX, London, UK
    FEBS Lett 516:265-9. 2002
    ..However the observation that binding of Mg-ATP to PKCdelta could protect the enzyme from dephosphorylation by PP2A(c) in vitro indicates that an additional input/factor is required for dephosphorylation in vivo...
  44. ncbi request reprint A high-content, cell-based screen identifies micropolyin, a new inhibitor of microtubule dynamics
    Manu De Rycker
    Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, London, UK
    Chem Biol Drug Des 73:599-610. 2009
    ..As our approach is unbiased, it should allow for discovery of new targets that may otherwise be overlooked...
  45. pmc Phosphatidylinositol 3-kinase C2alpha is essential for ATP-dependent priming of neurosecretory granule exocytosis
    Frederic A Meunier
    Lincoln s Inn Fields Laboratories, London Research Institute, Cancer Research UK, London WC2A 3PX, United Kingdom
    Mol Biol Cell 16:4841-51. 2005
    ..Based on these results, we propose that production of PtdIns3P by PI3K-C2alpha is required for acquisition of fusion competence in neurosecretion...
  46. pmc PKC controls HGF-dependent c-Met traffic, signalling and cell migration
    Stephanie Kermorgant
    Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO J 23:3721-34. 2004
    ..The dynamic properties conferred by the PKCepsilon control are shown to be essential for a normal HGF-dependent migratory response. Thus PKCs are shown to control both receptor traffic and signal traffic to relay HGF/c-Met responses...
  47. ncbi request reprint Receptor tyrosine kinase c-Met controls the cytoskeleton from different endosomes via different pathways
    Ludovic Ménard
    1 Centre for Tumour Biology, Barts Cancer Institute A Cancer Research UK Centre of Excellence, Queen Mary University of London, John Vane Science Centre, Charterhouse Square, London EC1M 6BQ, UK 2 Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Commun 5:3907. 2014
    ..Thus we describe a novel endosomal signalling mechanism whereby one signal output, Rac1, is stimulated through distinct pathways by the same RTK depending on which endosome it is localized to in the cell. ..
  48. ncbi request reprint Prognostic value of an activation state marker for epidermal growth factor receptor in tissue microarrays of head and neck cancer
    Anthony Kong
    Cell Biophysics Lab, London Research Institute, Cancer Research UK, London, UK
    Cancer Res 66:2834-43. 2006
    ..This powerful tool could be exploited as a new independent quantitative prognostic factor in clinical decisions and cancer management...
  49. ncbi request reprint Altered cleavage and localization of PINK1 to aggresomes in the presence of proteasomal stress
    Miratul M K Muqit
    Department of Molecular Neuroscience, Institute of Neurology, University College London, London, UK
    J Neurochem 98:156-69. 2006
    ..These observations provide valuable insights into the mechanisms of LB formation in PD that should lead to a better understanding of PD pathogenesis...
  50. pmc PKCepsilon-mediated phosphorylation of vimentin controls integrin recycling and motility
    Johanna Ivaska
    VTT Technical Research Centre for Finland, Medical Biotechnology and University of Turku Centre for Biotechnology, Turku, Finland
    EMBO J 24:3834-45. 2005
    ..Our results indicate that PKC-mediated phosphorylation of vimentin is a key process in integrin traffic through the cell...
  51. pmc Potentiation of protein kinase C zeta activity by 15-deoxy-delta(12,14)-prostaglandin J(2) induces an imbalance between mitogen-activated protein kinases and NF-kappa B that promotes apoptosis in macrophages
    Antonio Castrillo
    Instituto de Bioquimica, Centro Mixto CSIC UCM, Facultad de Farmacia, and Centro Nacional de Investigaciones Cardiovasculares, 28040 Madrid, Spain
    Mol Cell Biol 23:1196-208. 2003
    ....
  52. pmc Protein kinase C phosphorylates ribosomal protein S6 kinase betaII and regulates its subcellular localization
    Taras Valovka
    Ludwig Institute for Cancer Research, London W1W 7BS, United Kingdom
    Mol Cell Biol 23:852-63. 2003
    ..Taken together, this study uncovers a novel mechanism for the regulation of nucleocytoplasmic shuttling of S6KbetaII by PKC-mediated phosphorylation...
  53. pmc Site-directed perturbation of protein kinase C- integrin interaction blocks carcinoma cell chemotaxis
    Maddy Parsons
    Richard Dimbleby Cancer Research UK Department of Cancer Research, GKT School of Medicine, St Thomas Hospital, London SE1 7EH, United Kingdom
    Mol Cell Biol 22:5897-911. 2002
    ..Importantly, our findings outline a new concept as to how carcinoma cell chemotaxis is enhanced and provide a conceptual basis for interfering with tumor cell dissemination...
  54. pmc PKC epsilon controls the traffic of beta1 integrins in motile cells
    Johanna Ivaska
    Protein Phosphorylation Laboratory and Electron Microscopy Unit, Cancer Research UK London Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK Corresponding author e mail
    EMBO J 21:3608-19. 2002
    ..The evidence presented indicates that PKC epsilon controls an internal traffic step that under uninhibited conditions permits the recycling of beta 1 integrin, contributing to cell motility...
  55. pmc Calmodulin controls organization of the actin cytoskeleton via regulation of phosphatidylinositol (4,5)-bisphosphate synthesis in Saccharomyces cerevisiae
    Sylvane Desrivieres
    Division of Biochemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, Switzerland
    Biochem J 366:945-51. 2002
    ..Finally, the cmd1-226 mutant exhibits reduced levels of phosphatidylinositol (4,5)-bisphosphate. These findings suggest that calmodulin positively controls MSS4 activity and thereby the actin cytoskeleton...
  56. ncbi request reprint Differential activation of the PI 3-kinase effectors AKT/PKB and p70 S6 kinase by compound 48/80 is mediated by PKCalpha
    Richard D Byrne
    Division of Cell and Molecular Biology, Imperial College, London SW7 2AZ, United Kingdom
    Cell Signal 19:321-9. 2007
    ..The latter is only activated by higher doses of c48/80 resulting in an inhibition of the c48/80 induced PKB phosphorylation, thus explaining the observed biphasic activation profile for PKB in response to this secretagogue...
  57. pmc Integrin-specific signaling pathways controlling focal adhesion formation and cell migration
    Zohreh Mostafavi-Pour
    School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
    J Cell Biol 161:155-67. 2003
    ....
  58. ncbi request reprint Protein kinase C controls microtubule-based traffic but not proteasomal degradation of c-Met
    Stephanie Kermorgant
    Protein Phosphorylation Laboratory and Light Microscopy Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 278:28921-9. 2003
    ..Thus susceptibility to proteasomal degradation is not a consequence of post-endocytic traffic. The data define a PKC-controlled traffic pathway for c-Met that operates independently of its degradative pathway...
  59. ncbi request reprint PKCalpha reduces the lipid kinase activity of the p110alpha/p85alpha PI3K through the phosphorylation of the catalytic subunit
    Szabolcs Sipeki
    Semmelweis University, Department of Medical Chemistry, Molecular Biology and Pathobiochemistry 9 Puskin St, Budapest, Hungary
    Biochem Biophys Res Commun 339:122-5. 2006
    ..We conclude that PKCalpha is able to modulate negatively the lipid kinase activity of the p110alpha/p85alpha PI3K through the phosphorylation of the catalytic subunit...
  60. ncbi request reprint Molecular dissection of the interaction between the small G proteins Rac1 and RhoA and protein kinase C-related kinase 1 (PRK1)
    Darerca Owen
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    J Biol Chem 278:50578-87. 2003
    ..Surprisingly, as well as residues adjacent to Switch I, in Switch II, and in helix alpha5, it appears that the C-terminal stretch of basic amino acids in Rac is required for a high affinity interaction with HR1b...
  61. ncbi request reprint The tumour suppressor RASSF1A is a novel substrate of PKC
    Sunil K Verma
    Department of Medical Oncology, Medical Sciences Division, The University of Oxford, Oxford, UK
    FEBS Lett 582:2270-6. 2008
    ..By contrast, the equivalent AA mutant of RASSF1A phenocopied the WT protein. These findings indicate that PKC phosphorylation of RASSF1A regulates its ability to reorganize the microtubule network...
  62. pmc Emerging and diverse roles of protein kinase C in immune cell signalling
    Seng Lai Tan
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    Biochem J 376:545-52. 2003
    ..The importance of PKCs in cellular immune responses suggests that improved understanding of the molecular events that govern their actions could point to new avenues for development of treatments for immune disorders...
  63. ncbi request reprint PIKfyve negatively regulates exocytosis in neurosecretory cells
    Shona L Osborne
    Molecular Dynamics of Synaptic Function Laboratory, Queensland Brain Institute and School of Biomedical Sciences, University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia
    J Biol Chem 283:2804-13. 2008
    ..These results demonstrate a novel inhibitory role for PIKfyve catalytic activity in regulated secretion and provide further evidence for a fine tuning of exocytosis by 3-phosphorylated phosphoinositides...
  64. pmc PtdIns-specific MPR pathway association of a novel WD40 repeat protein, WIPI49
    Tim R Jeffries
    Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3PX, United Kingdom
    Mol Biol Cell 15:2652-63. 2004
    ..We conclude that WIPI49 is a novel regulatory component of the endosomal and MPR pathway and that this role is dependent upon the PI-binding properties of its WD40 domain...
  65. pmc Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors
    Stephen K Dove
    School of Biosciences, University of Birmingham, Birmingham, UK
    EMBO J 23:1922-33. 2004
    ..Svp1p is not involved in the contributions of FAB1/PtdIns(3,5)P2 to MVB sorting or to vacuole acidification and so additional PtdIns(3,5)P2 effectors must exist...
  66. ncbi request reprint Nucleotide binding by the Mdm2 RING domain facilitates Arf-independent Mdm2 nucleolar localization
    Masha V Poyurovsky
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Mol Cell 12:875-87. 2003
    ..We propose that nucleotide binding-facilitated nucleolar localization of Mdm2 is an evolutionarily conserved regulator of Mdm2 activity...