Nicholas Parham

Summary

Affiliation: Health Protection Agency
Location: Cambridge, UK
Summary:
Molecular diagnostics
Publications:
L. Zhang, N. J. Parham, F. Zhang, M. Aasa-Chapman, E. A. Gould and H. Zhang. Vaccination with coxsackievirus B3 Virus-Like Particles elicits humoral immune response and protects mice against myocarditis. (2012) Vaccine 30(13):2301-2308.

Nicholas J Parham, Surendra A Parmar, Navin Kumar, Sani Aliyu, Martin D Curran and Hongyi Zhang. Automated nucleic acid isolation in viral molecular diagnostics: Evaluation of the QIAsymphony® SP. (2012) The British Journal of Biomedical Science 69(1):18-25.

François J. Picard, Martin Gagnon, Marthe R. Bernier, Nicholas J. Parham, Martine Bastien, Maurice Boissinot, Régis Peytavi, and Michel G. Bergeron. Internal control for nucleic acid testing based on the use of purified Bacillus atrophaeus subsp. globigii spores. (2009) J. Clin. Microbiol. 47(3):751-7.

Sandra Isabel, Éric Leblanc, Maurice Boissinot, Dominique K. Boudreau, Myrian Grondin, François J. Picard, Eric A. Martel, Nicholas J. Parham, Patrick S. G. Chain, Douglas E. Bader, Michael R. Mulvey, Louis Bryden, Paul H. Roy, Marc Ouellette and Michel G. Bergeron. Divergence Among Genes Encoding the Elongation Factor Tu of Yersinia Species. (2008) Journal of Bacteriology 190: 7548-58.

Parham, N.J., Picard, F.J., Peytavi, R, Gagnon, M., Seyrig, G., Gagné, P-A and Bergeron, M.G. Specific magnetic bead-based capture of genomic DNA from clinical samples: application to the detection of group B streptococci in vaginal/anal swabs. (2007) Clin. Chem. 53(9), 1570-6.

Parham, N.J., Pollard, S.J., Chaudhuri, R.R., Beatson, S.A., Desvaux, M., Russell, M.A., Ruiz, J., Fivian, A., Vila, J. and Henderson, I.R. Prevalence of pathogenicity island II CFT073 genes among extraintestinal clinical isolates of Escherichia coli. (2005) J. Clin. Microbiol. 43(5), 2425-34.

Parham, N.J., Pollard, S.J., Desvaux, M., Scott-Tucker, A., Liu, C., Fivian, A. and Henderson, I.R. Distribution of the serine protease autotransporters of the Enterobacteriaceae among extraintestinal clinical isolates of Escherichia coli. (2005) J. Clin. Microbiol. 43(8), 4076-82.

Parham, N.J., Srinivasan, U., Desvaux, M., Foxman, B., Marrs, C.F. and Henderson, I.R. PicU, a second serine protease autotransporter of uropathogenic Escherichia coli. (2004) FEMS Microbiol. Lett. 230(1), 73-83.

Desvaux, M., Parham, N.J. and Henderson, I.R. Type V protein secretion: simplicity gone awry? (2004) Current Issues Mol. Biol. 6(2), 111-124.

Desvaux, M., Parham, N.J., Scott-Tucker, A. and Henderson, I.R. The general secretory pathway: a general misnomer? (2004) Trends Microbiol. 12(7), 306-309.

Desvaux, M., Parham, N.J. et Henderson, I.R. Le système de sécrétion de type V chez les bactéries Gram-négatives. (2003) Biofutur 237, 34-37.

Desvaux, M., Parham, N.J. and Henderson, I.R. The autotransporter secretion system. (2003) Research in Microbiology. 155(2), 53-60.

Parham, N., Spencer, J., Taylor, D., Ternent, H., Innocent, G., Mellor, D., Roberts, M. and Williams, A. An adapted ImmunoMagnetic cell Separation method for use in quantification of Escherichia coli O157:H7 from bovine faeces. (2003) J. Microbiol. Methods 53(1), 1-9.

Parham, N., Spencer, J., Roberts, M., Taylor, D. and Williams, A. The use of immunomagnetic cell separation for isolation of Escherichia coli O157:H7. (2001) Res. Vet. Sci. 70(Suppl. A), 11.

Spencer, J., Parham, N., Roberts, M., Taylor, D. and Williams, A. Adherence mechanisms of human diarrhoeagenic Escherichia coli and carriage of E. coli O157 in bovines. (2001) Res. Vet. Sci. 70(Suppl. A), 11.

Parham, N., Roberts, M. and Williams, A. Controlling E. coli O157:H7 disease in man – reducing the risk of exposure. (2000) Immunology News 7.2(April), 72-71.

Parham, N.J. and Gibson, G.R. Microbes involved in dissimilatory nitrate reduction in the human large intestine. (2000) FEMS Microbiol. Ecol. 31(1), 21-28.

Publications

  1. ncbi request reprint Specific magnetic bead based capture of genomic DNA from clinical samples: application to the detection of group B streptococci in vaginal/anal swabs
    Nicholas J Parham
    Centre de recherche en infectiologie de l Université Laval, Centre hospitalier universitaire de Québec Pavillon CHUL, Quebec, Canada
    Clin Chem 53:1570-6. 2007
  2. ncbi request reprint An adapted ImmunoMagnetic cell separation method for use in quantification of Escherichia coli O157:H7 from bovine faeces
    Nick Parham
    Department of Veterinary Pathology, Institute of Comparative Medicine, Glasgow University Veterinary School, Bearsden Road, Glasgow G61 1QH, UK
    J Microbiol Methods 53:1-9. 2003
  3. pmc Internal control for nucleic acid testing based on the use of purified Bacillus atrophaeus subsp. globigii spores
    Francois J Picard
    Centre de recherche en infectiologie de l Université Laval, Centre hospitalier universitaire de Québec Pavillon CHUL, Quebec, Quebec, Canada
    J Clin Microbiol 47:751-7. 2009
  4. pmc Divergence among genes encoding the elongation factor Tu of Yersinia Species
    Sandra Isabel
    Centre de recherche en infectiologie de l Université Laval, Centre Hospitalier Universitaire de Quebec, Quebec, Canada G1V 4G2
    J Bacteriol 190:7548-58. 2008
  5. pmc Distribution of the serine protease autotransporters of the Enterobacteriaceae among extraintestinal clinical isolates of Escherichia coli
    Nick J Parham
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Clin Microbiol 43:4076-82. 2005
  6. ncbi request reprint The general secretory pathway: a general misnomer?
    Mickael Desvaux
    Institute for Biomedical Research, The University of Birmingham The Medical School, Division of Immunity and Infection, Bacterial Pathogenesis and Genomics Unit, Vincent Drive, Edgbaston, Birmingham, UK B15 2TT
    Trends Microbiol 12:306-9. 2004
  7. ncbi request reprint Type V protein secretion: simplicity gone awry?
    Mickael Desvaux
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, The University of Birmingham, The Medical School, Edgbaston, Birmingham B15 2TT, UK
    Curr Issues Mol Biol 6:111-24. 2004
  8. ncbi request reprint The autotransporter secretion system
    Mickael Desvaux
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Res Microbiol 155:53-60. 2004
  9. ncbi request reprint PicU, a second serine protease autotransporter of uropathogenic Escherichia coli
    Nick J Parham
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Edgbaston, UK
    FEMS Microbiol Lett 230:73-83. 2004
  10. ncbi request reprint Automated nucleic acid isolation in viral molecular diagnostics: evaluation of the QIAsymphony SP
    N J Parham
    Health Protection Agency, Clinical Microbiology and Public Health Laboratory, Addenbrooke s Hospital, Box 236, Hills Road, Cambridge CB2 0QW, UK
    Br J Biomed Sci 69:18-25. 2012

Collaborators

Detail Information

Publications10

  1. ncbi request reprint Specific magnetic bead based capture of genomic DNA from clinical samples: application to the detection of group B streptococci in vaginal/anal swabs
    Nicholas J Parham
    Centre de recherche en infectiologie de l Université Laval, Centre hospitalier universitaire de Québec Pavillon CHUL, Quebec, Canada
    Clin Chem 53:1570-6. 2007
    ..To facilitate the adaptation of this method for point-of-care testing, we have developed a specific and efficient GBS DNA capture method that is compatible with both PCR and nonamplification detection technologies...
  2. ncbi request reprint An adapted ImmunoMagnetic cell separation method for use in quantification of Escherichia coli O157:H7 from bovine faeces
    Nick Parham
    Department of Veterinary Pathology, Institute of Comparative Medicine, Glasgow University Veterinary School, Bearsden Road, Glasgow G61 1QH, UK
    J Microbiol Methods 53:1-9. 2003
    ..We conclude that a 6.2-microm diameter Aureon bead can be used for quantitative isolation of E. coli O157:H7 directly from bovine faeces and, for this purpose, is preferred to the 2.8-microm diameter Dynal bead...
  3. pmc Internal control for nucleic acid testing based on the use of purified Bacillus atrophaeus subsp. globigii spores
    Francois J Picard
    Centre de recherche en infectiologie de l Université Laval, Centre hospitalier universitaire de Québec Pavillon CHUL, Quebec, Quebec, Canada
    J Clin Microbiol 47:751-7. 2009
    ..atrophaeus spores allowed validation of both the DNA extraction procedure and the amplification and detection process. Use of a spore-based control also provides a universal control for microbial cell lysis...
  4. pmc Divergence among genes encoding the elongation factor Tu of Yersinia Species
    Sandra Isabel
    Centre de recherche en infectiologie de l Université Laval, Centre Hospitalier Universitaire de Quebec, Quebec, Canada G1V 4G2
    J Bacteriol 190:7548-58. 2008
    ..Furthermore, they provided valuable phylogenetic information for possible reclassification and identification of Yersinia species...
  5. pmc Distribution of the serine protease autotransporters of the Enterobacteriaceae among extraintestinal clinical isolates of Escherichia coli
    Nick J Parham
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Clin Microbiol 43:4076-82. 2005
    ..coli and the ECOR collection, demonstrating an association between the presence of the SPATEs, including Vat, and uropathogenic E. coli phylogroups. In addition, we describe the distribution of SPATEs among nonpathogenic E. coli...
  6. ncbi request reprint The general secretory pathway: a general misnomer?
    Mickael Desvaux
    Institute for Biomedical Research, The University of Birmingham The Medical School, Division of Immunity and Infection, Bacterial Pathogenesis and Genomics Unit, Vincent Drive, Edgbaston, Birmingham, UK B15 2TT
    Trends Microbiol 12:306-9. 2004
    ..By putting the record straight and using novel findings within the field of bacterial protein secretion, we hope to bring clarity to this area of science and prevent further promulgation of incorrect terminologies...
  7. ncbi request reprint Type V protein secretion: simplicity gone awry?
    Mickael Desvaux
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, The University of Birmingham, The Medical School, Edgbaston, Birmingham B15 2TT, UK
    Curr Issues Mol Biol 6:111-24. 2004
    ....
  8. ncbi request reprint The autotransporter secretion system
    Mickael Desvaux
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Res Microbiol 155:53-60. 2004
    ..The autotransporter secretion process involving first the translocation of the precursor through the inner membrane and then its translocation through the outer membrane via a pore formed by a beta-barrel is reviewed...
  9. ncbi request reprint PicU, a second serine protease autotransporter of uropathogenic Escherichia coli
    Nick J Parham
    Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Edgbaston, UK
    FEMS Microbiol Lett 230:73-83. 2004
    ..One protein, designated PicU, was homologous to the Pic protein identified in Shigella flexneri and enteroaggregative E. coli. The PicU protein was expressed and investigated for functional activity...
  10. ncbi request reprint Automated nucleic acid isolation in viral molecular diagnostics: evaluation of the QIAsymphony SP
    N J Parham
    Health Protection Agency, Clinical Microbiology and Public Health Laboratory, Addenbrooke s Hospital, Box 236, Hills Road, Cambridge CB2 0QW, UK
    Br J Biomed Sci 69:18-25. 2012
    ..The results demonstrate that the QIAsymphony SP is suitable for use in routine virology molecular diagnostics, and provides a high-throughput capacity, which is needed in peak seasons of infection or in centralised laboratories...