Muñoz S, Minamino M, Casas Delucchi C, Patel H, Uhlmann F. A Role for Chromatin Remodeling in Cohesin Loading onto Chromosomes. Mol Cell. 2019;: pubmed publisher
..These observations demonstrate the importance of nucleosome-free DNA for cohesin loading and provide insight into how cohesin accesses DNA during its varied chromosomal activities. ..
Kataria M, Mouilleron S, Seo M, Corbi Verge C, Kim P, Uhlmann F. A PxL motif promotes timely cell cycle substrate dephosphorylation by the Cdc14 phosphatase. Nat Struct Mol Biol. 2018;25:1093-1102 pubmed publisher
..Our results illustrate the sophistication of phosphatase-substrate interactions and identify them as an important determinant of ordered cell cycle progression. ..
Touati S, Kataria M, Jones A, Snijders A, Uhlmann F. Phosphoproteome dynamics during mitotic exit in budding yeast. EMBO J. 2018;37: pubmed publisher
..These findings revise our view of mitotic exit and portray it as a dynamic process in which a range of mitotic kinases contribute to order both protein dephosphorylation and phosphorylation. ..
Thadani R, Kamenz J, Heeger S, Muñoz S, Uhlmann F. Cell-Cycle Regulation of Dynamic Chromosome Association of the Condensin Complex. Cell Rep. 2018;23:2308-2317 pubmed publisher
..We propose that condensation requires fine-tuned dynamic condensin interactions with more than one DNA. These results enhance our molecular understanding of condensin function during chromosome condensation. ..
Thadani R, Uhlmann F. Chromosome condensation: weaving an untangled web. Curr Biol. 2015;25:R663-6 pubmed publisher
..Two new studies characterise, both inÂ vivo and inÂ vitro, the essential contribution of the vertebrate condensin complex to chromosome organisation. ..
Murayama Y, Uhlmann F. DNA Entry into and Exit out of the Cohesin Ring by an Interlocking Gate Mechanism. Cell. 2015;163:1628-40 pubmed publisher
..Our results provide a model for dynamic DNA binding by all members of the SMC family and explain how lysine acetylation of cohesin establishes enduring sister chromatid cohesion. ..
Ocampo Hafalla M, Muñoz S, Samora C, Uhlmann F. Evidence for cohesin sliding along budding yeast chromosomes. Open Biol. 2016;6: pubmed publisher
..In this way, stable cohesin binding to DNA and enduring sister chromatid cohesion become compatible with simultaneous underlying chromosomal activities, including but maybe not limited to transcription. ..
Samora C, Saksouk J, Goswami P, Wade B, Singleton M, Bates P, et al
. Ctf4 Links DNA Replication with Sister Chromatid Cohesion Establishment by Recruiting the Chl1 Helicase to the Replisome. Mol Cell. 2016;63:371-84 pubmed publisher
..Our results reveal how Ctf4 forms a replisomal interaction hub that coordinates replication fork progression and sister chromatid cohesion establishment. ..
Godfrey M, Touati S, Kataria M, Jones A, Snijders A, Uhlmann F. PP2ACdc55 Phosphatase Imposes Ordered Cell-Cycle Phosphorylation by Opposing Threonine Phosphorylation. Mol Cell. 2017;65:393-402.e3 pubmed publisher
..They also unveil a regulatory principle based on the phosphoacceptor amino acid, which is likely to apply to signaling pathways beyond cell-cycle control. ..