Research TopicsGenomes and GenesSpecies | Cole TrapnellSummaryPublications
| Collaborators
|
Detail Information
Publications
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and CufflinksCole Trapnell
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA
Nat Protoc 7:562-78. 2012..The protocol's execution time depends on the volume of transcriptome sequencing data and available computing resources but takes less than 1 d of computer time for typical experiments and ∼1 h of hands-on time...
Improving RNA-Seq expression estimates by correcting for fragment biasAdam Roberts
Department of Computer Science, 387 Soda Hall, UC Berkeley, Berkeley, CA 94720, USA
Genome Biol 12:R22. 2011..We find improvements in expression estimates as measured by correlation with independently performed qRT-PCR and show that correction of bias leads to improved replicability of results across libraries and sequencing technologies...
Long noncoding RNAs regulate adipogenesisLei Sun
Whitehead Institute for Biomedical Research, Cambridge, MA 02142
Proc Natl Acad Sci U S A 110:3387-92. 2013..RNAi-mediated loss of function screens identified functional lncRNAs with varying impact on adipogenesis. Collectively, we have identified numerous lncRNAs that are functionally required for proper adipogenesis...
Differential analysis of gene regulation at transcript resolution with RNA-seqCole Trapnell
Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA
Nat Biotechnol 31:46-53. 2013..Cuffdiff 2 performs robust differential analysis in RNA-seq experiments at transcript resolution, revealing a layer of regulation not readily observable with other high-throughput technologies...
Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclassesMoran N Cabili
Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts 02142, USA
Genes Dev 25:1915-27. 2011..Our integrated, comprehensive, yet conservative reference catalog of human lincRNAs reveals the global properties of lincRNAs and will facilitate experimental studies and further functional classification of these genes...
Computational methods for transcriptome annotation and quantification using RNA-seqManuel Garber
Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts, USA
Nat Methods 8:469-77. 2011..Finally, we highlight the interdependence between these categories and discuss the benefits for different biological applications...
