Interphase fluorescence in situ hybridization detection of chromosome 17 and 17q region gains in neuroblastoma: are they secondary events?Luba Trakhtenbrot
Department of Pediatric Hemato Oncology and Institute of Hematology, The Chaim Sheba Medical Center, Tel Hashomer, 52621 Israel
Cancer Genet Cytogenet 137:95-101. 2002
..We suggest that chromosome 17 and 17q gains are not a primary event in the development of neuroblastoma...
Correlation between losses of IGH or its segments and deletions of 13q14 in t(11;14) (q13;q32) multiple myelomaLuba Trakhtenbrot
Division of Hematology and the Sheba Cancer Research Center, The Chaim Sheba Medical Center, Tel Hashomer, and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
Genes Chromosomes Cancer 49:17-27. 2010
..In contrast, we did not find any association between Delta13 and deletions of the IGH gene or its segments in the MM patients with t(4;14)(p16;q32)...
- Clonal expansion and not cell interconversion is the basis for the neuroblast and nonneuronal types of the SK-N-SH neuroblastoma cell line
Ninette Cohen
Department of Pediatric Hemato-Oncology and Institute of Hematology, The Chaim Sheba Medical Center, Tel Hashomer, Israel
Cancer Genet Cytogenet 143:80-4. 2003
..Under certain conditions, the less-aggressive S cells can dominate over the highly aggressive N cells, which eventually lead to the formation of the SH-EP and vice-versa...
- SKY detection of chromosome rearrangements in two cases of tMDS with a complex karyotype
Ninette Cohen
Institute of Hematology and Pediatric Hemato Oncology, The Chaim Sheba Medical Center, Tel Hashomer, Israel
Cancer Genet Cytogenet 138:128-32. 2002
..We clearly present that the use of SKY combined with conventional G-banding analysis and FISH has assisted in the identification of important chromosomal events that may play a key role in the development of t-MDS...
- Assessment of the response to imatinib in chronic myeloid leukemia patients--comparison between the FISH, multiplex and RT-PCR methods
Pia Raanani
The Institute of Hematology, The Chaim Sheba Medical Center, Tel Hashomer, Israel
Eur J Haematol 73:243-50. 2004
....
- Chromosome 13q deletion and IgH abnormalities may be both masked by near-tetraploidy in a high proportion of multiple myeloma patients: a combined morphology and I-FISH analysis
Maya Koren-Michowitz
Division of Hematology, The Sheba Cancer Research Center, The Chaim Sheba Medical Center, Tel Hashomer, Israel
Cancer Lett 255:307-14. 2007
..Therefore, we suggest that a search for NT should be added to the routine BM assessment in MM patients...
- The multiparametric scanning system for evaluation of minimal residual disease in hematological malignancies
Luba Trakhtenbrot
Department of Pediatric Hematology Oncology, Safra Children s Hospital and Institute of Hematology, The Chaim Sheba Medical Center, Tel Hashomer, and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
Acta Haematol 112:24-9. 2004
..Morphological analysis of small populations of cells related either to malignancy or recipient-associated markers may improve the accuracy of chimerism and MRD testing and delineate their clinical significance...
- SKY analysis of childhood neural tumors and cell lines demonstrates a susceptibility of aberrant chromosomes to further rearrangements
Racheli Stanchescu
Department of Pediatric Hemato Oncology and Cancer Research Center, The Edmond and Lily Safra Children s Hospital, Sheba Medical Center, Tel Hashomer, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
Cancer Lett 250:47-52. 2007
..9%, 54 of 1099 chromosomes). These results are highly suggestive that aberrant chromosomes in solid tumors have a higher susceptibility to accumulate further rearrangements than "normal" chromosomes...
- Acquisition of a Ph chromosome with minor BCR/ABL fusion in treatment-related myelodysplastic syndrome with chromosome 7 abnormalities in a patient treated for Hodgkin disease
Abraham Kneller
Institute of Hematology, The Chaim Sheba Medical Center, Tel Hashomer 52621, Israel
Cancer Genet Cytogenet 159:58-62. 2005
..We propose two possible models that may explain the appearance of the BCR/ABL fusion in the pre-existing treatment-related MDS clones characterized by chromosome 7 rearrangements...
- A role for common fragile site induction in amplification of human oncogenes
Asaf Hellman
Department of Genetics, The Life Sciences Institute, The Hebrew University, Jerusalem 91904, Israel
Cancer Cell 1:89-97. 2002
..Upon replication stress, this region showed perturbed chromatin organization, predisposing it to breakage. Thus, in vivo induction of CFSs can play an important role in human oncogenesis...
- Karyotypic evolution pathways in medulloblastoma/primitive neuroectodermal tumor determined with a combination of spectral karyotyping, G-banding, and fluorescence in situ hybridization
Ninette Cohen
Department of Pediatric Hemato-Oncology and Institute of Hematology, The Chaim Sheba Medical Center, Tel Hashomer 52621, Israel
Cancer Genet Cytogenet 149:44-52. 2004
....
- Combined analysis of morphology and fluorescence in situ hybridization in follow-up of minimal residual disease in a child with Philadelphia-positive acute lymphoblastic leukemia
Bella Bielorai
Department of Pediatric Hemato-Oncology and Institute of Hematology, The Chaim Sheba Medical Center, Tel-Hashomer and Sackler School of Medicine, Tel-Aviv University, 5262, Tel-Aviv, Israel
Cancer Genet Cytogenet 138:64-8. 2002
..Moreover, since mature differentiated cells have a low proliferative capacity, there is a low risk for relapse...
- SKY reveals a high frequency of unbalanced translocations involving chromosome 6 in t(12;21)-positive acute lymphoblastic leukemia
David R Betts
Department of Oncology, University Children s Hospital, Steinwiesstrasse 75, CH 8032 Zurich, Switzerland
Leuk Res 32:39-43. 2008
..The results show that rearrangements of 6p are also non-random events t(12;21)-positive ALL. This study illustrates the value of a combined SKY and G-banding approach in identifying novel karyotypic events in childhood ALL...
- Detection of non-diploid cells in premalignant and malignant oral lesions using combined morphological and FISH analysis - a new method for early detection of suspicious oral lesions
Abraham Hirshberg
Department of Oral Pathology and Oral Medicine, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel
Cancer Lett 253:282-90. 2007
..We suggest that the combined morphological and cytogenetic analysis of cells collected by a non-invasive brush sampling can enhance early detection of potentially malignant cells...
- Simultaneous detection of deletions of 9q and 22q in a subgroup of chronic myelocytic leukemia Philadelphia-positive patients by a novel probe
Ninette Cohen
Cancer Genet Cytogenet 141:89-90. 2003
- Comparison of triple helical COMBO-FISH and standard FISH by means of quantitative microscopic image analysis of abl/bcr positions in cell nuclei
Jutta Schwarz Finsterle
Kirchhoff Institute of Physics, University of Heidelberg, Im Neuenheimer Feld 227, D 69120 Heidelberg, Germany
J Biochem Biophys Methods 70:397-406. 2007
..For precise quantitative microscopic analysis of genomic loci, fluorescence labelling procedures are recommended that well maintain the native chromatin topology. Triple helical COMBO-FISH may offer such an approach...
- COMBO-FISH for focussed fluorescence labelling of gene domains: 3D-analysis of the genome architecture of abl and bcr in human blood cells
Jutta Schwarz-Finsterle
Kirchhoff-Institute of Physics, University of Heidelberg, Im Neuenheimer Feld 227, D-69120 Heidelberg, Germany
Cell Biol Int 29:1038-46. 2005
..These results indicate that COMBO-FISH may be more sensitive than standard FISH in case of slight modifications in the genome architecture...
- Co-existence of multiple subclones in TEL-AML1 at diagnosis of acute lymphoblastic leukaemia in association with submicroscopic deletion of AML1
Rachel Rothman
Department of Paediatric Haemato-Oncology and Institute of Haematology, The Edmond and Lily Safra Children Hospital, The Chaim Sheba Medical Centre, Tel-Hashomer, Israel
Br J Haematol 129:491-8. 2005
..The presence of numerous subclones in about 25% of patients with TEL/AML1+ ALL suggests extensive clonal evolution by the time of diagnosis...
- Philadelphia-chromosome-positive T-lymphoblastic leukemia: acute leukemia or chronic myelogenous leukemia blastic crisis
Pia Raanani
Institute of Hematology, Sheba Medical Center and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
Acta Haematol 113:181-9. 2005
..Although both entities carry a severe prognosis, differentiating between them might have clinical relevance, especially in the imatinib era...
- Determination of chromosome 13 status in bone marrow cells of patients with multiple myeloma using combined morphologic and fluorescence in situ hybridization analysis
Izhar Hardan
Department of Bone Marrow Transplantation, Hematology and the Sheba Cancer Research Center, The Chaim Sheba Medical Center, Tel-Hashomer, Israel
Exp Hematol 32:254-60. 2004
..We suggest this technique as a novel sensitive and specific method for detection of del13q in a minor PC population of MM patients...
- Reciprocal relationship between a Ph-negative clone with trisomy 8 associated with severe myelodysplasia and a Ph-positive clone following imatinib treatment in a patient with accelerated-phase chronic myelogenous leukemia (CML)
Paulina Patchenko
Am J Hematol 77:420. 2004
