Stephen Toddo

Summary

Publications

  1. doi request reprint Improved production of membrane proteins in Escherichia coli by selective codon substitutions
    Morten H H Nørholm
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle 6, DK 2970 Hørsholm, Denmark Electronic address
    FEBS Lett 587:2352-8. 2013
  2. pmc Application of split-green fluorescent protein for topology mapping membrane proteins in Escherichia coli
    Stephen Toddo
    Department of Biochemistry and Biophysics, Center for Biomembrane Research, Stockholm University, SE 10691 Sweden
    Protein Sci 21:1571-6. 2012

Collaborators

  • Sara Light
  • Morten H H Nørholm
  • Daniel O Daley
  • Minttu T I Virkki
  • Gunnar von Heijne

Detail Information

Publications2

  1. doi request reprint Improved production of membrane proteins in Escherichia coli by selective codon substitutions
    Morten H H Nørholm
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Alle 6, DK 2970 Hørsholm, Denmark Electronic address
    FEBS Lett 587:2352-8. 2013
    ..We conclude that coding sequences can be re-wired for high-level protein expression by selective engineering of the 5' coding sequence with synonymous codons, thus circumventing the need to consider whole sequence optimization. ..
  2. pmc Application of split-green fluorescent protein for topology mapping membrane proteins in Escherichia coli
    Stephen Toddo
    Department of Biochemistry and Biophysics, Center for Biomembrane Research, Stockholm University, SE 10691 Sweden
    Protein Sci 21:1571-6. 2012
    ..Here, we show that the bimolecular split-green fluorescent protein complementation system can overcome this limitation and can be used to determine the location of both the N- and C-termini of inner membrane proteins in Escherichia coli...