Research Topics
Genomes and GenesSpecies | Marcel PrinsSummaryAffiliation: Wageningen University Country: The Netherlands Publications
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Detail Information
Publications
Strategies for antiviral resistance in transgenic plantsMarcel Prins
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD, Wageningen, The Netherlands
Mol Plant Pathol 9:73-83. 2008..In a final summarizing section, the potential risks involved in the introduction of transgenic crops and the specifics of the approaches used will be discussed...
A novel class of heat-responsive small RNAs derived from the chloroplast genome of Chinese cabbage (Brassica rapa)Lu Wang
National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
BMC Genomics 12:289. 2011....- Potatovirus X and Tobacco mosaic virus-based vectors compatible with the Gateway cloning systemCristiano Lacorte
Laboratory of Virology, Wageningen University, Wageningen, The Netherlands
J Virol Methods 164:7-13. 2010..A PVX-GW transient expression assay of a nucleocapsid protein gene fragment of Tomato spotted wilt virus and of a single chain antibody against this protein was shown to confer effective resistance to TSWV infection... - Tomato chlorotic mottle virus is a target of RNA silencing but the presence of specific short interfering RNAs does not guarantee resistance in transgenic plantsSimone G Ribeiro
Laboratory of Virology, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
J Virol 81:1563-73. 2007.... - Binding of small interfering RNA molecules is crucial for RNA interference suppressor activity of rice hoja blanca virus NS3 in plantsHans Hemmes
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
J Gen Virol 90:1762-6. 2009..This indicates that siRNA binding of NS3 is indeed essential for the suppressor function of NS3 and that residues at positions 173-175 are involved in the siRNA binding and suppressor activities... 
Multiple virus resistance at a high frequency using a single transgene constructEtienne Bucher
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
J Gen Virol 87:3697-701. 2006..Thus RNA silencing can be further improved for high frequency multiple virus resistance by combining small RNA fragments from a series of target viruses...- The NS3 protein of Rice hoja blanca tenuivirus suppresses RNA silencing in plant and insect hosts by efficiently binding both siRNAs and miRNAsHans Hemmes
Laboratory of Virology, Wageningen University, Wageningen, The Netherlands
RNA 13:1079-89. 2007..In addition, NS3 has a high affinity for miRNA/miRNA* duplexes, indicating that its activity might also interfere with miRNA-regulated gene expression in both insects and plants... - The influenza A virus NS1 protein binds small interfering RNAs and suppresses RNA silencing in plantsEtienne Bucher
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
J Gen Virol 85:983-91. 2004..In addition, NS1 was shown to be capable of binding siRNAs. The data presented here fit with a potential role for NS1 in counteracting innate antiviral responses in vertebrates by sequestering siRNAs... - Diverging affinity of tospovirus RNA silencing suppressor proteins, NSs, for various RNA duplex moleculesEsther Schnettler
Wageningen University, Laboratory of Virology, Droevendaalsesteeg 1, 6708 PB Wageningen, Netherlands
J Virol 84:11542-54. 2010..The observed affinity to long dsRNA for only a subset of the tospoviruses studied is discussed in light of evolutional divergence and their ancestral relation to the animal-infecting members of the Bunyaviridae... - The nucleoprotein of Tomato spotted wilt virus as protein tag for easy purification and enhanced production of recombinant proteins in plantsCristiano Lacorte
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
Protein Expr Purif 55:17-22. 2007..These results show that the homopolymerization properties of the N protein can be used as a fast and simple way to purify large amounts of proteins from plants... - Assessing the expression of chicken anemia virus proteins in plantsCristiano Lacorte
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
Virus Res 129:80-6. 2007..Overall, our results show that all CAV proteins can be expressed in plant cells, though expression level of VP1 needs to be further optimized before testing its potential as (edible) subunit vaccine... - Resistance mechanisms to plant viruses: an overviewRob Goldbach
Laboratory of Virology, Wageningen University, Binnenhaven 11, PD 6709 Wageningen, The Netherlands
Virus Res 92:207-12. 2003..This paper will give a brief overview of the major strategies that have become operational during the past 10 years... - Broad virus resistance in transgenic plantsMarcel Prins
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
Trends Biotechnol 21:373-5. 2003..Increased understanding of the molecular biology of virus infection is starting to bear fruit, enabling specific strategies to be designed for virus resistance in crops... - The NS3 protein of rice hoja blanca virus suppresses RNA silencing in mammalian cellsEsther Schnettler
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands
J Gen Virol 89:336-40. 2008..The possible applications of this protein in modulating RNAi and investigating the proposed antiviral RNAi response in mammalian cell systems are discussed... - Phage display-selected single-chain antibodies confer high levels of resistance against Tomato spotted wilt virusMarcel Prins
Laboratory of Virology, Wageningen University, The Netherlands
J Gen Virol 86:2107-13. 2005..Protoplast experiments provided evidence that the two resistance-conferring antibodies may have a different mode of action and could be combined for higher durability of resistance in the field... - Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positionsEtienne Bucher
Laboratory of Virology, Wageningen University, The Netherlands
J Virol 77:1329-36. 2003..These results indicate that plant-infecting negative-strand RNA viruses carry a gene for a suppressor of RNA silencing... - Inter- and intramolecular recombinations in the cucumber mosaic virus genome related to adaptation to alstroemeriaYuh-Kun Chen
Laboratory of Virology, Wageningen University, Wageningen, The Netherlands
J Virol 76:4119-24. 2002..Competition experiments revealed that these recombined RNA 2 and 3 segments increased the biological fitness of CMV in alstroemeria...