Michel M Klerks

Summary

Affiliation: Wageningen University
Country: The Netherlands

Publications

  1. ncbi Comparison of real-time PCR methods for detection of Salmonella enterica and Escherichia coli O157:H7, and introduction of a general internal amplification control
    M M Klerks
    Wageningen University and Research Centre, Plant Research International BV, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    J Microbiol Methods 59:337-49. 2004
  2. ncbi Comparison of methods of extracting Salmonella enterica serovar Enteritidis DNA from environmental substrates and quantification of organisms by using a general internal procedural control
    M M Klerks
    Plant Research International BV, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    Appl Environ Microbiol 72:3879-86. 2006
  3. ncbi Physiological and molecular responses of Lactuca sativa to colonization by Salmonella enterica serovar Dublin
    M M Klerks
    Wageningen University and Research Centre, Plant Research International BV, Droevendaalsesteeg 1, 6709 PB Wageningen, The Netherlands
    Appl Environ Microbiol 73:4905-14. 2007
  4. ncbi Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency
    Michel M Klerks
    Wageningen University and Research Centre, Plant Research International BV, Wageningen, The Netherlands
    ISME J 1:620-31. 2007
  5. ncbi Quantification of contamination of lettuce by GFP-expressing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium
    Eelco Franz
    Biological Farming Systems Group, Department of Plant Sciences, Wageningen University and Research Center, Marijkeweg 22, 6709 PG Wageningen, The Netherlands
    Food Microbiol 24:106-12. 2007
  6. ncbi Prevalence of Shiga toxin-producing Escherichia coli stx1, stx2, eaeA, and rfbE genes and survival of E. coli O157:H7 in manure from organic and low-input conventional dairy farms
    Eelco Franz
    Wageningen University and Research Centre, Biological Farming Systems Group, Marijkeweg 22, 6709 PG Wageningen, The Netherlands
    Appl Environ Microbiol 73:2180-90. 2007
  7. ncbi Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel internal amplification control approach using Biotrove OpenArrays
    R Van Doorn
    Plant Research International B V, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    Appl Environ Microbiol 75:7253-60. 2009
  8. ncbi Development of a multiplex AmpliDet RNA for the simultaneous detection of Potato leafroll virus and Potato virus Y in potato tubers
    M M Klerks
    Plant Research International BV, PO Box 16, 6700 AA Wageningen, The Netherlands
    J Virol Methods 93:115-25. 2001

Collaborators

Detail Information

Publications8

  1. ncbi Comparison of real-time PCR methods for detection of Salmonella enterica and Escherichia coli O157:H7, and introduction of a general internal amplification control
    M M Klerks
    Wageningen University and Research Centre, Plant Research International BV, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    J Microbiol Methods 59:337-49. 2004
    ..The adjusted methods have the potential to provide fast and sensitive detection of Salmonella spp. or E. coli O157:H7, enabling accurate quantification and preventing false negative results by using the general IAC...
  2. ncbi Comparison of methods of extracting Salmonella enterica serovar Enteritidis DNA from environmental substrates and quantification of organisms by using a general internal procedural control
    M M Klerks
    Plant Research International BV, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    Appl Environ Microbiol 72:3879-86. 2006
    ..Finally, the study presents a more accurate molecular analysis for quantification of serovar Enteritidis initially present in soil or manure using DNA extraction and TaqMan PCR...
  3. ncbi Physiological and molecular responses of Lactuca sativa to colonization by Salmonella enterica serovar Dublin
    M M Klerks
    Wageningen University and Research Centre, Plant Research International BV, Droevendaalsesteeg 1, 6709 PB Wageningen, The Netherlands
    Appl Environ Microbiol 73:4905-14. 2007
    ..In addition, it was confirmed that Salmonella spp. can colonize the interior of lettuce plants, thus potentially imposing a human health risk when processed and consumed...
  4. ncbi Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency
    Michel M Klerks
    Wageningen University and Research Centre, Plant Research International BV, Wageningen, The Netherlands
    ISME J 1:620-31. 2007
    ..Finally, a tentative route of infection is described that includes plant-microbe factors, herewith enabling further design of preventive strategies...
  5. ncbi Quantification of contamination of lettuce by GFP-expressing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium
    Eelco Franz
    Biological Farming Systems Group, Department of Plant Sciences, Wageningen University and Research Center, Marijkeweg 22, 6709 PG Wageningen, The Netherlands
    Food Microbiol 24:106-12. 2007
    ....
  6. ncbi Prevalence of Shiga toxin-producing Escherichia coli stx1, stx2, eaeA, and rfbE genes and survival of E. coli O157:H7 in manure from organic and low-input conventional dairy farms
    Eelco Franz
    Wageningen University and Research Centre, Biological Farming Systems Group, Marijkeweg 22, 6709 PG Wageningen, The Netherlands
    Appl Environ Microbiol 73:2180-90. 2007
    ....
  7. ncbi Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel internal amplification control approach using Biotrove OpenArrays
    R Van Doorn
    Plant Research International B V, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    Appl Environ Microbiol 75:7253-60. 2009
    ..The developed IAC is ideally suited for high-throughput screenings of, for example, ecological and agricultural samples on next-generation qPCR platforms...
  8. ncbi Development of a multiplex AmpliDet RNA for the simultaneous detection of Potato leafroll virus and Potato virus Y in potato tubers
    M M Klerks
    Plant Research International BV, PO Box 16, 6700 AA Wageningen, The Netherlands
    J Virol Methods 93:115-25. 2001
    ..The multiplex assay detected clearly the viruses present originally in the potato tubers in all samples, demonstrating its potential for routine diagnostic work and high-throughput screening...