Henry Kariithi

Summary

Affiliation: Wageningen University
Location: Wageningen, The Netherlands
Summary:
My current research focuses on the infectomics, genomics, proteomics and pathobiology of the Glossina hytrosavirus, a nucleocytoplasmic large dsDNA virus pathogenic to tsetse flies (Diptera: Glossinidae). The virus has recently been accommodated in the new Hytrosaviridae family of insect viruses. Laboratory stocks of G. pallidipes flies infected by Glossina hytrosavirus have hypertrophied salivary glands and midgut epithelial cells, gonadal/ovarian anomalies, distorted sex-ratios, and reduced insemination rates, fecundity and lifespan. Often, Glossina virus infections cause collapse of G. pallidipes colonies. These symptoms are rare in the wild populations of G. pallidipes, as well as in other wild or colonized tsetse species. The primary goal of my research is to investigate the functional/structural genomics and proteomics of the virus, as a prerequisite to development of strategies to manage viral infections in G. pallidipes colonies. Another equally important goal of my research is to investigate the (molecular) mechanisms that control the unique pathology of Glossina hytrosavirus, how the host’s defence system responds to infection by the virus, and how the microbiome modulate the outcomes of the viral infection process.

Publications

  1. doi Dynamics of the salivary gland hypertrophy virus in laboratory colonies of Glossina pallidipes (Diptera: Glossinidae)
    Adly M M Abd-Alla
    Insect Pest Control Laboratory, Joint FAO IAEA Programme of Nuclear Techniques in Food and Agriculture, Vienna, Austria
    Virus Res 150:103-10. 2010
  2. doi Proteomic analysis of Glossina pallidipes salivary gland hypertrophy virus virions for immune intervention in tsetse fly colonies
    Henry M Kariithi
    Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    J Gen Virol 91:3065-74. 2010
  3. pmc The salivary secretome of the tsetse fly Glossina pallidipes (Diptera: Glossinidae) infected by salivary gland hypertrophy virus
    Henry M Kariithi
    Laboratory of Virology, Wageningen University, Wageningen, The Netherlands
    PLoS Negl Trop Dis 5:e1371. 2011
  4. doi Prevalence and genetic variation of salivary gland hypertrophy virus in wild populations of the tsetse fly Glossina pallidipes from southern and eastern Africa
    H M Kariithi
    Insect Pest Control Laboratory, Joint FAO IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, Vienna, Austria
    J Invertebr Pathol 112:S123-32. 2013
  5. doi Correlation between structure, protein composition, morphogenesis and cytopathology of Glossina pallidipes salivary gland hypertrophy virus
    Henry M Kariithi
    Laboratory of Virology, Wageningen University, Wageningen, The Netherlands
    J Gen Virol 94:193-208. 2013
  6. pmc Transgenerational transmission of the Glossina pallidipes hytrosavirus depends on the presence of a functional symbiome
    Drion G Boucias
    Entomology and Nematology Department, University of Florida, Gainesville, Florida, USA
    PLoS ONE 8:e61150. 2013
  7. pmc Managing hytrosavirus infections in Glossina pallidipes colonies: feeding regime affects the prevalence of salivary gland hypertrophy syndrome
    Adly M M Abd-Alla
    Insect Pest Control Laboratory, Joint FAO IAEA Programme of Nuclear Techniques in Food and Agriculture, Vienna, Austria
    PLoS ONE 8:e61875. 2013
  8. doi Proteomic footprints of a member of Glossinavirus (Hytrosaviridae): an expeditious approach to virus control strategies in tsetse factories
    Henry M Kariithi
    Insect Pest Control Laboratory, Joint FAO IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, Vienna, Austria
    J Invertebr Pathol 112:S26-31. 2013

Collaborators

Detail Information

Publications8

  1. doi Dynamics of the salivary gland hypertrophy virus in laboratory colonies of Glossina pallidipes (Diptera: Glossinidae)
    Adly M M Abd-Alla
    Insect Pest Control Laboratory, Joint FAO IAEA Programme of Nuclear Techniques in Food and Agriculture, Vienna, Austria
    Virus Res 150:103-10. 2010
    ....
  2. doi Proteomic analysis of Glossina pallidipes salivary gland hypertrophy virus virions for immune intervention in tsetse fly colonies
    Henry M Kariithi
    Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    J Gen Virol 91:3065-74. 2010
    ..This result suggests that immune intervention of viral infections in colonies of G. pallidipes is a realistic option...
  3. pmc The salivary secretome of the tsetse fly Glossina pallidipes (Diptera: Glossinidae) infected by salivary gland hypertrophy virus
    Henry M Kariithi
    Laboratory of Virology, Wageningen University, Wageningen, The Netherlands
    PLoS Negl Trop Dis 5:e1371. 2011
    ..However, secretion of host proteins is of equal importance for successful transmission and requires cataloging of G. pallidipes secretome proteins from hypertrophied and non-hypertrophied SGs...
  4. doi Prevalence and genetic variation of salivary gland hypertrophy virus in wild populations of the tsetse fly Glossina pallidipes from southern and eastern Africa
    H M Kariithi
    Insect Pest Control Laboratory, Joint FAO IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, Vienna, Austria
    J Invertebr Pathol 112:S123-32. 2013
    ..The current study suggests that the proposed molecular-based virus management strategies have a good prospect of working throughout eastern and southern Africa due to the low diversity of the GpSGHV strains...
  5. doi Correlation between structure, protein composition, morphogenesis and cytopathology of Glossina pallidipes salivary gland hypertrophy virus
    Henry M Kariithi
    Laboratory of Virology, Wageningen University, Wageningen, The Netherlands
    J Gen Virol 94:193-208. 2013
    ..This study provides important information about GpSGHV biology and suggests options for the development of future anti-GpSGHV strategies by interfering with virus-host interactions...
  6. pmc Transgenerational transmission of the Glossina pallidipes hytrosavirus depends on the presence of a functional symbiome
    Drion G Boucias
    Entomology and Nematology Department, University of Florida, Gainesville, Florida, USA
    PLoS ONE 8:e61150. 2013
    ..Our data suggest that GpSGHV has co-evolved with the tsetse symbiome and that the symbionts play key roles in the virus transmission from mother to progeny...
  7. pmc Managing hytrosavirus infections in Glossina pallidipes colonies: feeding regime affects the prevalence of salivary gland hypertrophy syndrome
    Adly M M Abd-Alla
    Insect Pest Control Laboratory, Joint FAO IAEA Programme of Nuclear Techniques in Food and Agriculture, Vienna, Austria
    PLoS ONE 8:e61875. 2013
    ..Taken together, these data indicate that the clean feeding approach is applicable in large-scale G. pallidipes production facilities and eliminates the deleterious effects of the virus and the SGH syndrome in these colonies...
  8. doi Proteomic footprints of a member of Glossinavirus (Hytrosaviridae): an expeditious approach to virus control strategies in tsetse factories
    Henry M Kariithi
    Insect Pest Control Laboratory, Joint FAO IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, Vienna, Austria
    J Invertebr Pathol 112:S26-31. 2013
    ..The relevance of the proteome findings to tsetse and trypanosomiasis management in sub-Sahara Africa is also considered...