Jules Beekwilder

Summary

Affiliation: Wageningen University
Country: The Netherlands

Publications

  1. ncbi request reprint Antioxidants in raspberry: on-line analysis links antioxidant activity to a diversity of individual metabolites
    Jules Beekwilder
    Plant Research International, Postbus 16, 6700 AA Wageningen, The Netherlands
    J Agric Food Chem 53:3313-20. 2005
  2. ncbi request reprint Identification and dietary relevance of antioxidants from raspberry
    Jules Beekwilder
    Plant Research International, Wageningen, The Netherlands
    Biofactors 23:197-205. 2005
  3. ncbi request reprint Microbial production of natural raspberry ketone
    Jules Beekwilder
    Plant Research International, Wageningen, The Netherlands
    Biotechnol J 2:1270-9. 2007
  4. pmc Reconstitution of the costunolide biosynthetic pathway in yeast and Nicotiana benthamiana
    Qing Liu
    Laboratory of Plant Physiology, Wageningen University, Wageningen, The Netherlands
    PLoS ONE 6:e23255. 2011
  5. pmc Nicotiana benthamiana as a production platform for artemisinin precursors
    Teun W J M van Herpen
    Laboratory of Plant Physiology, Wageningen University, Wageningen, The Netherlands
    PLoS ONE 5:e14222. 2010
  6. pmc A broad set of different llama antibodies specific for a 16 kDa heat shock protein of Mycobacterium tuberculosis
    Anke K Trilling
    Plant Research International, Wageningen, The Netherlands
    PLoS ONE 6:e26754. 2011
  7. pmc De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae
    Frank Koopman
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
    Microb Cell Fact 11:155. 2012
  8. pmc Characterization of Rhamnosidases from Lactobacillus plantarum and Lactobacillus acidophilus
    Jules Beekwilder
    Plant Research International, Wageningen, The Netherlands
    Appl Environ Microbiol 75:3447-54. 2009
  9. pmc The impact of the absence of aliphatic glucosinolates on insect herbivory in Arabidopsis
    Jules Beekwilder
    Plant Research International, Wageningen, The Netherlands
    PLoS ONE 3:e2068. 2008
  10. pmc Production of resveratrol in recombinant microorganisms
    Jules Beekwilder
    Plant Research International, 6700 AA Wageningen, The Netherlands
    Appl Environ Microbiol 72:5670-2. 2006

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Antioxidants in raspberry: on-line analysis links antioxidant activity to a diversity of individual metabolites
    Jules Beekwilder
    Plant Research International, Postbus 16, 6700 AA Wageningen, The Netherlands
    J Agric Food Chem 53:3313-20. 2005
    ..This information will assist in the future development and identification of raspberry lines with enhanced health-promoting properties...
  2. ncbi request reprint Identification and dietary relevance of antioxidants from raspberry
    Jules Beekwilder
    Plant Research International, Wageningen, The Netherlands
    Biofactors 23:197-205. 2005
    ....
  3. ncbi request reprint Microbial production of natural raspberry ketone
    Jules Beekwilder
    Plant Research International, Wageningen, The Netherlands
    Biotechnol J 2:1270-9. 2007
    ..These results therefore lay a strong foundation for a potentially renewable source of "natural" flavour compounds making use of plant genes...
  4. pmc Reconstitution of the costunolide biosynthetic pathway in yeast and Nicotiana benthamiana
    Qing Liu
    Laboratory of Plant Physiology, Wageningen University, Wageningen, The Netherlands
    PLoS ONE 6:e23255. 2011
    ..benthamiana leaves resulted in costunolide production of up to 60 ng.g(-1) FW. In addition, two new compounds were formed that were identified as costunolide-glutathione and costunolide-cysteine conjugates...
  5. pmc Nicotiana benthamiana as a production platform for artemisinin precursors
    Teun W J M van Herpen
    Laboratory of Plant Physiology, Wageningen University, Wageningen, The Netherlands
    PLoS ONE 5:e14222. 2010
    ..Here the potential of this plant is explored for rapid production of precursors of artemisinin, a sesquiterpenoid compound that is used for malaria treatment...
  6. pmc A broad set of different llama antibodies specific for a 16 kDa heat shock protein of Mycobacterium tuberculosis
    Anke K Trilling
    Plant Research International, Wageningen, The Netherlands
    PLoS ONE 6:e26754. 2011
    ..Due to the small size and stable nature of llama antibody domains selected antibodies can serve as a detection reagent in multiplexed and sensitive assays for M. tuberculosis...
  7. pmc De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae
    Frank Koopman
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
    Microb Cell Fact 11:155. 2012
    ..Thus to overcome these limitations, metabolic engineering of specific pathway in microbial systems have been envisaged to produce high quantity of a single molecules...
  8. pmc Characterization of Rhamnosidases from Lactobacillus plantarum and Lactobacillus acidophilus
    Jules Beekwilder
    Plant Research International, Wageningen, The Netherlands
    Appl Environ Microbiol 75:3447-54. 2009
    ..lactis appeared to be a limiting factor, rhamnose removal was confirmed, indicating that rhamnosidase-producing bacteria may find commercial application, depending on the technological properties of the strains and enzymes...
  9. pmc The impact of the absence of aliphatic glucosinolates on insect herbivory in Arabidopsis
    Jules Beekwilder
    Plant Research International, Wageningen, The Netherlands
    PLoS ONE 3:e2068. 2008
    ..6 fold higher on the double myb28myb29 mutant completely lacking aliphatic glucosinolates and 1.8 higher on the single mutants with intermediate levels of aliphatic glucosinolates compared to wild type plants...
  10. pmc Production of resveratrol in recombinant microorganisms
    Jules Beekwilder
    Plant Research International, 6700 AA Wageningen, The Netherlands
    Appl Environ Microbiol 72:5670-2. 2006
    ..When p-coumaric acid was used as the precursor, resveratrol accumulations in the culture medium were observed to be comparable in E. coli (16 mg/liter) and yeast (6 mg/liter)...
  11. ncbi request reprint Metabolism of carotenoids and apocarotenoids during ripening of raspberry fruit
    Jules Beekwilder
    Plant Research International, Wageningen, The Netherlands
    Biofactors 34:57-66. 2008
    ..Such information helps us to better understand how these compounds are produced in plants and may also enable us to develop novel strategies for improved apocarotenoid production in fruits or indeed, alternative production systems...
  12. pmc Functional characterization of enzymes forming volatile esters from strawberry and banana
    Jules Beekwilder
    Plant Research International, 6700 AA Wageningen, The Netherlands
    Plant Physiol 135:1865-78. 2004
    ..This confirmed that the availability of alcohol substrates is an important parameter to consider when engineering volatile ester formation in plants...
  13. ncbi request reprint Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis)
    Katarina Cankar
    Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    FEBS Lett 588:1001-7. 2014
    ..Formation of (+)-nootkatone was detected at 144±10μg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases. ..
  14. pmc Non-smoky glycosyltransferase1 prevents the release of smoky aroma from tomato fruit
    Yury M Tikunov
    Plant Research International, 6700 AA Wageningen, The Netherlands
    Plant Cell 25:3067-78. 2013
    ..NSGT1 may be used in a precision breeding strategy toward development of tomato fruits with distinct flavor phenotypes...
  15. doi request reprint The metabolite chemotype of Nicotiana benthamiana transiently expressing artemisinin biosynthetic pathway genes is a function of CYP71AV1 type and relative gene dosage
    Hieng Ming Ting
    Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands
    New Phytol 199:352-66. 2013
    ....
  16. pmc 16 kDa heat shock protein from heat-inactivated Mycobacterium tuberculosis is a homodimer - suitability for diagnostic applications with specific llama VHH monoclonals
    Saurabh K Srivastava
    Plant Research International, Wageningen, The Netherlands
    PLoS ONE 8:e64040. 2013
    ..tb. bacteria in sputum or cultures it may represent a useful tool. Recently, a broad set of twelve 16 kDa specific heavy chain llama antibodies (VHH) has been isolated, and their utility for diagnostic applications was explored...
  17. doi request reprint A chicory cytochrome P450 mono-oxygenase CYP71AV8 for the oxidation of (+)-valencene
    Katarina Cankar
    Laboratory of Plant Physiology, Wageningen University and Research Centre, Wageningen, The Netherlands
    FEBS Lett 585:178-82. 2011
    ..Likewise, amorpha-4,11-diene was converted to artemisinic acid. Surprisingly, the chicory P450 has a different regio-specificity on (+)-valencene compared to germacrene A and amorpha-4,11-diene...
  18. pmc The mQTL hotspot on linkage group 16 for phenolic compounds in apple fruits is probably the result of a leucoanthocyanidin reductase gene at that locus
    Sabaz Ali Khan
    Wageningen UR Plant Breeding, P, O, Box 16, Wageningen, 6700 AA, The Netherlands
    BMC Res Notes 5:618. 2012
    ....
  19. ncbi request reprint Isoprenoid biosynthesis in Artemisia annua: cloning and heterologous expression of a germacrene A synthase from a glandular trichome cDNA library
    Cinzia M Bertea
    Plant Research International, P O Box 16, 6700 AA Wageningen, The Netherlands
    Arch Biochem Biophys 448:3-12. 2006
    ..These results demonstrate the potential of the use of A. annua glandular trichomes as a starting material for studying isoprenoid biosynthesis in this plant species...
  20. pmc The flavonoid pathway in tomato seedlings: transcript abundance and the modeling of metabolite dynamics
    Marian Groenenboom
    Biometris, Wageningen University and Research Center, Wageningen, The Netherlands
    PLoS ONE 8:e68960. 2013
    ..Importantly, this is possible under the biologically relevant restriction that the enzymatic properties estimated by the model are conserved between the tissues. ..
  21. doi request reprint Valencene synthase from the heartwood of Nootka cypress (Callitropsis nootkatensis) for biotechnological production of valencene
    Jules Beekwilder
    Plant Research International, Wageningen, the Netherlands Platform Green Synthetic Biology, Delft University of Technology, Delft, The Netherlands
    Plant Biotechnol J 12:174-82. 2014
    ..In an optimized Rhodobacter sphaeroides strain, expression of CnVS increased valencene yields 14-fold to 352 mg/L, bringing production to levels with industrial potential...
  22. ncbi request reprint Antibody orientation on biosensor surfaces: a minireview
    Anke K Trilling
    Plant Research International, Wageningen, The Netherlands
    Analyst 138:1619-27. 2013
    ..Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application...
  23. doi request reprint Cloning and characterisation of a maize carotenoid cleavage dioxygenase (ZmCCD1) and its involvement in the biosynthesis of apocarotenoids with various roles in mutualistic and parasitic interactions
    Zhongkui Sun
    Plant Research International, P O Box 16, 6700 AA, Wageningen, The Netherlands
    Planta 228:789-801. 2008
    ..ZmCCD1 is proposed to be involved in cyclohexenone and mycorradicin formation in mycorrhizal maize roots but not in strigolactone formation...
  24. ncbi request reprint Orientation of llama antibodies strongly increases sensitivity of biosensors
    Anke K Trilling
    Plant Research International, Wageningen, The Netherlands Laboratory of Organic Chemistry, Wageningen University, Wageningen, The Netherlands
    Biosens Bioelectron 60:130-6. 2014
    ..This technique may increase the containment of infectious diseases such as FMDV as its strongly enhanced sensitivity may facilitate early diagnostics. ..
  25. doi request reprint Affinity of Avr2 for tomato cysteine protease Rcr3 correlates with the Avr2-triggered Cf-2-mediated hypersensitive response
    John W Van't Klooster
    Wageningen University, Laboratory of Phytopathology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    Mol Plant Pathol 12:21-30. 2011
    ..Overall, our data suggest that the affinity of the Avr2 mutants for Rcr3 correlates with their ability to trigger a Cf-2-mediated HR...
  26. ncbi request reprint Co-evolution of insect proteases and plant protease inhibitors
    Maarten A Jongsma
    Plant Research International, Wageningen University and Research Center WageningenUR, Droevendaalsesteeg 1, P O Box 619, 6700 AP Wageningen, The Netherlands
    Curr Protein Pept Sci 12:437-47. 2011
    ....
  27. doi request reprint Self-assembled functional organic monolayers on oxide-free copper
    Mabel A Caipa Campos
    Laboratory of Organic Chemistry, Wageningen University and Research Center, Dreijenplein 8, 6703 HB, Wageningen, The Netherlands
    Langmuir 27:8126-33. 2011
    ..In addition, the biotinylated copper surfaces were employed in the immobilization of biomolecules such as streptavidin...
  28. doi request reprint The effect of uniform capture molecule orientation on biosensor sensitivity: dependence on analyte properties
    Anke K Trilling
    Plant Research International, P O Box 16, 6700 AP Wageningen, The Netherlands
    Biosens Bioelectron 40:219-26. 2013
    ..The results indicate that orientation of the capture molecule hardly affects high-affinity interactions, while it leads to strong improvements in sensitivity for lower-affinity interactions...
  29. doi request reprint Polyphenol identification based on systematic and robust high-resolution accurate mass spectrometry fragmentation
    Justin J J van der Hooft
    Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands
    Anal Chem 83:409-16. 2011
    ..Accurate MS(n)-based spectral tree data are therefore a powerful tool to distinguish metabolites with similar elemental formula, thereby assisting compound identification in complex biological samples such as crude plant extracts...
  30. ncbi request reprint Identification and recombinant expression of a novel chymotrypsin from Spodoptera exigua
    Salvador Herrero
    Business Unit Bioscience, Plant Research International B V, Wageningen University and Research Centre, Wageningen, The Netherlands
    Insect Biochem Mol Biol 35:1073-82. 2005
    ..This suggests a possible role of this protein in the proteolytic remodelling that occurs in the gut during the larval to pupal molt...
  31. ncbi request reprint Selection by phage display of a variant mustard trypsin inhibitor toxic against aphids
    Luigi R Ceci
    Istituto di Biomembrane e Bioenergetica, Sezione di Trani, Via Corato 17, 70059 Trani, Italy
    Plant J 33:557-66. 2003
    ..The selected gene will allow the development of transgenic crops that are protected against sucking insect pests...
  32. ncbi request reprint Properties of purified gut trypsin from Helicoverpa zea, adapted to proteinase inhibitors
    Mariateresa Volpicella
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Bari, Italy
    Eur J Biochem 270:10-9. 2003
    ..The same inhibitors inhibited HzTrypsin-C with apparent equilibrium dissociation constants ranging from 1 nm to 30 nm. Thus, HzTrypsin-S seems to allow the insect to overcome different defensive proteinase inhibitors in plants...
  33. pmc Structural basis of the resistance of an insect carboxypeptidase to plant protease inhibitors
    Alex Bayes
    Departament de Bioquimica i Biologia Molecular, Facultat de Ciencies, and Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, Bellaterra, Spain
    Proc Natl Acad Sci U S A 102:16602-7. 2005
    ..This report explains a crystal structure of an insect protease and its adaptation to defensive plant protease inhibitors...
  34. pmc Expression of plant flavor genes in Lactococcus lactis
    Igor Hernández
    NIZO Food Research, P O Box 20, 6710 BA Ede, The Netherlands
    Appl Environ Microbiol 73:1544-52. 2007
    ..The results illustrate the potential of the application of L. lactis as a food grade expression platform for the recombinant production of proteins and bioactive compounds from plants...
  35. doi request reprint Changes in antioxidant and metabolite profiles during production of tomato paste
    Esra Capanoglu
    Faculty of Chemical and Metallurgical Engineering, Food Engineering Department, Istanbul Technical University, Maslak 34469, Istanbul, Turkey
    J Agric Food Chem 56:964-73. 2008
    ....
  36. doi request reprint Antioxidants, phenolic compounds, and nutritional quality of different strawberry genotypes
    Sara Tulipani
    Institute of Biochemistry, Faculty of Medicine, Department of Environmental and Crop Science SAPROV, Marche Polytechnic University, Ancona, Italy
    J Agric Food Chem 56:696-704. 2008
    ..The HPLC analyses confirmed qualitative and quantitative variability in the antioxidant profiles. These studies show that differences exist among cultivars, applicable in dietary studies in human subjects...
  37. ncbi request reprint Response of the digestive system of Helicoverpa zea to ingestion of potato carboxypeptidase inhibitor and characterization of an uninhibited carboxypeptidase B
    Alex Bayes
    Departament de Bioquimica i Biologia Molecular, Facultat de Ciències and Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, E 08193 Bellaterra, Spain
    Insect Biochem Mol Biol 36:654-64. 2006
    ..The substrate specificity of recombinant CPBHz was tested using peptides. Unlike other CPB enzymes, the enzyme appeared to be highly selective for C-terminal lysine residues. Inhibition by PCI appeared to be pH-dependent...
  38. ncbi request reprint Identification and characterization of digestive serine proteases from inhibitor-resistant Helicoverpa zea larval midgut
    Mariateresa Volpicella
    Department of Biochemistry and Molecular Biology, University of Bari, Via Amendola 165 A, 70126 Bari, Italy
    J Chromatogr B Analyt Technol Biomed Life Sci 833:26-32. 2006
    ..The sequence features of these proteases are discussed in relation to their sensitivity to inhibitors. The results provide insight in the enzymes deployed by Helicoverpa larvae to overcome plant defence...
  39. doi request reprint Stress-induced biosynthesis of dicaffeoylquinic acids in globe artichoke
    Andrea Moglia
    DIVAPRA, Plant Genetics and Breeding, University of Torino, Via L da Vinci 44, 10095 Grugliasco TO Italy
    J Agric Food Chem 56:8641-9. 2008
    ..On the basis of these results a role of dicaffeoylquinic acids in UV protection in globe artichoke is hypothesized...
  40. ncbi request reprint Phage display selects for amylases with improved low pH starch-binding
    Raymond M D Verhaert
    Pharmaceutical Biology, University Centre for Pharmacy, A Deusinglaan 1, 9713, AV Groningen, The Netherlands
    J Biotechnol 96:103-18. 2002
    ..5/7.5 that is improved relative to the wild type alpha-amylase. These data demonstrate that useful alpha-amylase mutants can be selected via surface display on the basis of their binding properties to starch at lowered pH values...