Paul J van Diest

Summary

Affiliation: VU University Medical Center
Country: The Netherlands

Publications

  1. doi Methylation profiles of hereditary and sporadic ovarian cancer
    Guus M Bol
    Department of Pathology, University Medical Centre Utrecht Cancer Centre, Utrecht, The Netherlands
    Histopathology 57:363-70. 2010
  2. ncbi Pathologic analysis of sentinel lymph nodes
    P J van Diest
    Department of Pathology, VU Medical Center, 1007 MB Amsterdam, The Netherlands
    Semin Surg Oncol 20:238-45. 2001
  3. ncbi No consent should be needed for using leftover body material for scientific purposes. For
    Paul J van Diest
    Department of Pathology, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, Netherlands
    BMJ 325:648-51. 2002
  4. pmc Cadaveric tissue donation: a pathologist's perspective
    P J van Diest
    Department of Pathology, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands
    J Med Ethics 29:135-6. 2003
  5. pmc HIF-1α overexpression in ductal carcinoma in situ of the breast in BRCA1 and BRCA2 mutation carriers
    Petra van der Groep
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 8:e56055. 2013
  6. doi Fibrotic focus and hypoxia in male breast cancer
    Robert Kornegoor
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 25:1397-404. 2012
  7. ncbi Discrimination between benign and malignant prostate tissue using chromatin texture analysis in 3-D by confocal laser scanning microscopy
    André Huisman
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Prostate 67:248-54. 2007
  8. ncbi Serum CA-125 in relation to adnexal dysplasia and cancer in women at hereditary high risk of ovarian cancer
    Brenda B J Hermsen
    Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, The Netherlands
    J Clin Oncol 25:1383-9. 2007
  9. pmc Loss of expression of FANCD2 protein in sporadic and hereditary breast cancer
    Petra van der Groep
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Breast Cancer Res Treat 107:41-7. 2008
  10. ncbi Analysis of copy number changes on chromosome 16q in male breast cancer by multiplex ligation-dependent probe amplification
    Miangela M Lacle
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Mod Pathol 26:1461-7. 2013

Detail Information

Publications129 found, 100 shown here

  1. doi Methylation profiles of hereditary and sporadic ovarian cancer
    Guus M Bol
    Department of Pathology, University Medical Centre Utrecht Cancer Centre, Utrecht, The Netherlands
    Histopathology 57:363-70. 2010
    ..The aim of this study was to investigate promoter methylation of a set of common tumour suppressor genes in BRCA1-related ovarian cancer in comparison with sporadic ovarian cancer...
  2. ncbi Pathologic analysis of sentinel lymph nodes
    P J van Diest
    Department of Pathology, VU Medical Center, 1007 MB Amsterdam, The Netherlands
    Semin Surg Oncol 20:238-45. 2001
    ..Further studies are necessary to establish the role of multiparameter flow cytometry and sophisticated molecular biological techniques such as reverse transcription polymerase chain reaction (RT-PCR) in detecting SLN metastases...
  3. ncbi No consent should be needed for using leftover body material for scientific purposes. For
    Paul J van Diest
    Department of Pathology, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, Netherlands
    BMJ 325:648-51. 2002
  4. pmc Cadaveric tissue donation: a pathologist's perspective
    P J van Diest
    Department of Pathology, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands
    J Med Ethics 29:135-6. 2003
  5. pmc HIF-1α overexpression in ductal carcinoma in situ of the breast in BRCA1 and BRCA2 mutation carriers
    Petra van der Groep
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 8:e56055. 2013
    ..Hypoxia-related proteins are therefore putative targets for therapy and molecular imaging for early detection and monitoring therapy response in BRCA mutation patients...
  6. doi Fibrotic focus and hypoxia in male breast cancer
    Robert Kornegoor
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 25:1397-404. 2012
    ..These markers seem to have similar clinical importance as previously reported in female breast cancer...
  7. ncbi Discrimination between benign and malignant prostate tissue using chromatin texture analysis in 3-D by confocal laser scanning microscopy
    André Huisman
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Prostate 67:248-54. 2007
    ..Confocal laser scanning microscopy (CLSM) allows performing measurements in nuclei reconstructed in 3-D. The aim of this study was to evaluate the clinical usefulness of 3-D texture analysis of prostate tissue...
  8. ncbi Serum CA-125 in relation to adnexal dysplasia and cancer in women at hereditary high risk of ovarian cancer
    Brenda B J Hermsen
    Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, The Netherlands
    J Clin Oncol 25:1383-9. 2007
    ..The aim of this study was to investigate whether CA-125 concentrations are indicative of adnexal dysplasia and cancer in women at hereditary high risk of ovarian/tubal cancer...
  9. pmc Loss of expression of FANCD2 protein in sporadic and hereditary breast cancer
    Petra van der Groep
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Breast Cancer Res Treat 107:41-7. 2008
    ..FANCD2 is of independent prognostic value in sporadic breast cancer...
  10. ncbi Analysis of copy number changes on chromosome 16q in male breast cancer by multiplex ligation-dependent probe amplification
    Miangela M Lacle
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Mod Pathol 26:1461-7. 2013
    ..Gain on 16p and loss of 16q identify a group of male breast cancer with low propensity to develop lymph node metastases. ..
  11. pmc Receptor conversion in distant breast cancer metastases
    Laurien D C Hoefnagel
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    Breast Cancer Res 12:R75. 2010
    ....
  12. doi Promoter hypermethylation patterns in fallopian tube epithelium of BRCA1 and BRCA2 germ line mutation carriers
    Jonathan G Bijron
    Department of Pathology, University Medical Centre Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Endocr Relat Cancer 19:69-81. 2012
    ..Methylation could, therefore, play an important role in the increased risk of gynaecological malignancies in BRCA mutation carriers...
  13. pmc Expression of hypoxia-inducible factor-1alpha and cell cycle proteins in invasive breast cancer are estrogen receptor related
    Reinhard Bos
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Breast Cancer Res 6:R450-9. 2004
    ..In this study we undertook a detailed evaluation of the relationships between HIF-1alpha and cell cycle-associated proteins...
  14. ncbi Methylation of the TWIST1 promoter, TWIST1 mRNA levels, and immunohistochemical expression of TWIST1 in breast cancer
    Eelke H Gort
    Department of Pathology, University Medical Center Utrecht, P O Box 85500, 3508 GA Utrecht, The Netherlands
    Cancer Epidemiol Biomarkers Prev 17:3325-30. 2008
    ..This indicates that although TWIST1 CpG methylation is useful as a biomarker in breast cancer diagnosis, there is no direct correlation with TWIST1 expression...
  15. ncbi Sentinel lymph node tumor load: an independent predictor of additional lymph node involvement and survival in melanoma
    Ronald J C L M Vuylsteke
    Department of Surgical Oncology, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands
    Ann Surg Oncol 12:440-8. 2005
    ..The aim of this study was to predict the absence of ALN metastases in patients with a positive SLN by using features of the primary melanoma and SLN tumor load...
  16. doi Accuracy of sentinel node biopsy after neoadjuvant chemotherapy in breast cancer patients: a systematic review
    Carolien H M van Deurzen
    Department of Pathology, University Medical Centre Utrecht, The Netherlands
    Eur J Cancer 45:3124-30. 2009
    ..This review was conducted to determine the accuracy of SN biopsy following NAC...
  17. doi Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3
    Arjan J Groot
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Mol Immunol 46:2022-8. 2009
    ..Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment...
  18. doi Molecular profiling of invasive breast cancer by multiplex ligation-dependent probe amplification-based copy number analysis of tumor suppressor and oncogenes
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Mod Pathol 23:1029-39. 2010
    ....
  19. pmc Oncogene amplification in male breast cancer: analysis by multiplex ligation-dependent probe amplification
    Robert Kornegoor
    Department of Pathology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Breast Cancer Res Treat 135:49-58. 2012
    ..In addition CCND1 amplification seems to be an independent prognosticator in male breast cancer...
  20. doi Simultaneous detection of TOP2A and HER2 gene amplification by multiplex ligation-dependent probe amplification in breast cancer
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 23:62-70. 2010
    ..In conclusion, MLPA is an easy and accurate method to simultaneously detect breast cancer HER-2/neu and TopoII alpha copy number status in paraffin-embedded tissue, and thus an attractive supplement or alternative to CISH...
  21. doi Progression risk of columnar cell lesions of the breast diagnosed in core needle biopsies
    Anoek H J Verschuur-Maes
    Department of Pathology, University Medical Centre Utrecht, The Netherlands
    Int J Cancer 129:2674-80. 2011
    ..This illustrates that an atypical CCL in a CNB may signal the presence of concurrent lesions or development of advanced lesions in future and may justify ("mini") surgical excision...
  22. ncbi Conditional inactivation of HIF-1 using intrabodies
    Arjan J Groot
    Department of Pathology, University Medical Center Utrecht, 3508 GA, Utrecht, The Netherlands
    Cell Oncol 30:397-409. 2008
    ..Inducible intrabody targeting is a highly specific strategy for temporal protein inactivation and may have applications for disease treatment...
  23. pmc Non-sentinel lymph node metastases associated with isolated breast cancer cells in the sentinel node
    Carolien H M van Deurzen
    Department of Pathology, University Medical Center Utrecht, GA Utrecht, The Netherlands
    J Natl Cancer Inst 100:1574-80. 2008
    ....
  24. doi Fallopian tube intraluminal tumor spread from noninvasive precursor lesions: a novel metastatic route in early pelvic carcinogenesis
    Jonathan G Bijron
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Am J Surg Pathol 37:1123-30. 2013
    ..Although rare in the absence of invasive cancer, we show that intraluminal shedding of tumor cells in the fallopian tubes from serous carcinoma cases are common and a likely route of abdominal spread. ..
  25. doi Immunophenotyping of male breast cancer
    Robert Kornegoor
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Histopathology 61:1145-55. 2012
    ..Male breast cancer is a rare disease, and knowledge of carcinogenesis is limited. Conflicting results, based on small series, have been reported for clinically relevant biomarkers...
  26. pmc Nuclear Kaiso expression is associated with high grade and triple-negative invasive breast cancer
    Jeroen F Vermeulen
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 7:e37864. 2012
    ..In conclusion, our data indicate that nuclear Kaiso is common in clinically aggressive ductal breast cancer, while cytoplasmic Kaiso and a p120-mediated relief of Kaiso-dependent transcriptional repression characterize ILC...
  27. doi Molecular subtyping of male breast cancer by immunohistochemistry
    Robert Kornegoor
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 25:398-404. 2012
    ..This distribution of molecular subtypes in male breast cancer is clearly different compared with female breast cancers, pointing to possible important differences in carcinogenesis...
  28. ncbi Discrimination between benign and malignant prostate biopsies using three-dimensional chromatin texture analysis by confocal laser scanning microscopy
    André Huisman
    Department of Pathology, University Medical Center Utrecht, P O Box 85500, 3508 GA Utrecht, The Netherlands
    Anal Quant Cytol Histol 33:265-70. 2011
    ..To evaluate the clinical usefulness of computing three-dimensional (3-D) nuclear texture features on prostate biopsy specimens to discriminate among benign, prostatic intraepithelial neoplasia (PIN), and malignant specimens...
  29. ncbi High frequency of HIF-1alpha overexpression in BRCA1 related breast cancer
    Petra van der Groep
    Department of Pathology, University Medical Center, 3508 GA, Utrecht, The Netherlands
    Breast Cancer Res Treat 111:475-80. 2008
    ..This points to an important role of hypoxia and its key regulator HIF-1alpha in hereditary breast carcinogenesis...
  30. ncbi The sentinel node in cervical cancer: scintigraphy and laparoscopic gamma probe-guided biopsy
    Rik Pijpers
    Department of Nuclear Medicine, VU University Medical Center, Amsterdam, The Netherlands
    Eur J Nucl Med Mol Imaging 31:1479-86. 2004
    ..Based on SN histology, the treatment was altered in nine patients (26%). We conclude that laparoscopic SN biopsy is feasible in cervical cancer and may result in custom-designed treatment strategies with a reduction in morbidity...
  31. doi Amplification testing in breast cancer by multiplex ligation-dependent probe amplification of microdissected tissue
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Methods Mol Biol 755:107-18. 2011
    ..This chapter describes a method for MLPA analysis using invasive breast tumor cells acquired by laser capture microdissection. This protocol can also be applied to MLPA analysis of preinvasive lesions and metastases...
  32. doi Whole slide images for primary diagnostics of gastrointestinal tract pathology: a feasibility study
    Shaimaa Al-Janabi
    Department of Pathology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Hum Pathol 43:702-7. 2012
    ..Up-front histopathologic diagnosis of gastrointestinal biopsies and resections can be done on whole slide images...
  33. doi Columnar cell lesions on breast needle biopsies: is surgical excision necessary? A systematic review
    Anoek H J Verschuur-Maes
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    Ann Surg 255:259-65. 2012
    ..This systematic review was conducted to provide treatment recommendations for patients with a diagnosis of columnar cell lesions (CCLs) in a breast core needle biopsy (CNB)...
  34. pmc Lympho-vascular invasion in BRCA related breast cancer compared to sporadic controls
    Marise R Heerma van Voss
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    BMC Cancer 10:145. 2010
    ....
  35. ncbi No amplifications of hypoxia-inducible factor-1alpha gene in invasive breast cancer: a tissue microarray study
    Marije M Vleugel
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Cell Oncol 26:347-51. 2004
    ..The aim of this study was to evaluate the presence of HIF-1alpha gene amplifications in invasive breast cancer as an explanation for HIF-1alpha protein overexpression...
  36. ncbi Protein expression of B-cell lymphoma gene 6 (BCL-6) in invasive breast cancer is associated with cyclin D1 and hypoxia-inducible factor-1alpha (HIF-1alpha)
    Reinhard Bos
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Oncogene 22:8948-51. 2003
    ..The cases with amplification in BCL-6 showed an increased (25%) incidence of BCL-6 protein overexpression. Thus, this study is the first to show that BCL-6 oncogene activation plays a role in cancers other than lymphomas...
  37. doi Frequent promoter hypermethylation of BRCA2, CDH13, MSH6, PAX5, PAX6 and WT1 in ductal carcinoma in situ and invasive breast cancer
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Heidelberglaan 100, Utrecht, The Netherlands
    J Pathol 225:222-31. 2011
    ..Promoter hypermethylation of PAX6, BRCA2, PAX5, WT1, CDH13 and MSH6 seems to be a frequent early event in breast cancer and methylation levels of GSTP1 (and CDKN2A, although still low) seem to increase with increasing DCIS grade...
  38. doi Morphometry of isolated tumor cells in breast cancer sentinel lymph nodes: metastases or displacement?
    Carolien H M van Deurzen
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    Am J Surg Pathol 33:106-10. 2009
    ..This supports the hypothesis that some of these deposits could represent benign epithelium or degenerated malignant cells lacking outgrowth potential...
  39. ncbi Development of 3D chromatin texture analysis using confocal laser scanning microscopy
    André Huisman
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cell Oncol 27:335-45. 2005
    ..The aim of this study was to develop 3D texture features that quantitatively describe changes in chromatin architecture associated with malignancy using CLSM images...
  40. pmc Expression of the stem cell marker ALDH1 in BRCA1 related breast cancer
    Marise R Heerma van Voss
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Cell Oncol (Dordr) 34:3-10. 2011
    ..Since ALDH1 is a marker of both mammary stem cells and breast cancer stem cells, we compared ALDH1 expression in malignant tissue of BRCA1 mutation carriers to non-carriers...
  41. pmc Expression of the RNA helicase DDX3 and the hypoxia response in breast cancer
    Guus M Bol
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    PLoS ONE 8:e63548. 2013
    ..However, the relation between DDX3 and hypoxia has not been addressed in human tumors. In this paper, we studied the relation between DDX3 and the hypoxic responsive proteins in human breast cancer...
  42. ncbi Implementation of accurate and fast DNA cytometry by confocal microscopy in 3D
    Lennert S Ploeger
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cell Oncol 27:225-30. 2005
    ..The aim of this study was to describe the conditions for accurate and fast 3D CLSM cytometry with a minimum of user interaction to arrive at sufficient throughput for pilot clinical applications...
  43. pmc Differential expression of growth factor receptors and membrane-bound tumor markers for imaging in male and female breast cancer
    Jeroen F Vermeulen
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 8:e53353. 2013
    ..We therefore investigated expression profiles of growth factor receptors and membrane-bound tumor markers in male breast cancer and gynecomastia, in comparison with female breast cancer...
  44. pmc Immunophenotyping invasive breast cancer: paving the road for molecular imaging
    Jeroen F Vermeulen
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    BMC Cancer 12:240. 2012
    ..Because development of new tracers is labor-intensive and costly, we searched for the smallest panel of tumor membrane markers that would allow detection of the wide spectrum of invasive breast cancers...
  45. ncbi c-Jun activation is associated with proliferation and angiogenesis in invasive breast cancer
    Marije M Vleugel
    Department of Pathology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Hum Pathol 37:668-74. 2006
    ..Our present results in breast cancer patients confirm this relation in vivo for the first time. Therefore, c-Jun/AP-1 targeting may provide new ways to block tumor angiogenesis...
  46. doi ESR1 amplification is rare in breast cancer and is associated with high grade and high proliferation: a multiplex ligation-dependent probe amplification study
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Anal Cell Pathol (Amst) 33:13-8. 2010
    ....
  47. pmc Poorly differentiated breast carcinoma is associated with increased expression of the human polycomb group EZH2 gene
    Frank M Raaphorst
    Department of Pathology, VU University Medical Center, BioCentrum Amsterdam, University of Amsterdam, Amsterdam, The Netherlands
    Neoplasia 5:481-8. 2003
    ..These observations suggest that deregulated expression of EZH2 is associated with loss of differentiation and development of poorly differentiated breast cancer in humans...
  48. ncbi Mutation analysis of the HIF-1alpha oxygen-dependent degradation domain in invasive breast cancer
    Marije M Vleugel
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Cancer Genet Cytogenet 163:168-72. 2005
    ..No mutations seem to occur in the ODD of hif-1alpha in HIF-1alpha overexpressing invasive breast cancer, which rules ODD mutations out as a possible explanation for the diffuse HIF-1alpha expression pattern often seen in this cancer...
  49. doi Pathology issues related to SN procedures and increased detection of micrometastases and isolated tumor cells
    Paul J van Diest
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    Breast Dis 31:65-81. 2010
    ..In this paper, we provide an up to date discussion on the virtues and flaws of these different methods to find SN metastases, and provide recommendations on the optimal pathology protocol for breast cancer SNs...
  50. ncbi Progressive deregulation of the cell cycle with higher tumor grade in the stroma of breast phyllodes tumors
    Arno Kuijper
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Am J Clin Pathol 123:690-8. 2005
    ..Several combinations of aberrantly expressed cell cycle proteins seem important in the stromal progression of phyllodes tumors. The number of stromal cell cycle aberrations and stromal p53 expression might predict clinical behavior...
  51. pmc Nuclear localization of the transcriptional coactivator YAP is associated with invasive lobular breast cancer
    Eva J Vlug
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA, Utrecht, The Netherlands
    Cell Oncol (Dordr) 36:375-84. 2013
    ..YAP overexpression has been associated with different types of cancer, such as lung, skin, prostate, ovary and liver cancer. Recently, YAP was linked to E-cadherin-dependent regulation of contact inhibition in breast cancer cells...
  52. pmc Near-infrared fluorescence molecular imaging of ductal carcinoma in situ with CD44v6-specific antibodies in mice: a preclinical study
    Jeroen F Vermeulen
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA, Utrecht, The Netherlands
    Mol Imaging Biol 15:290-8. 2013
    ..As CD44v6 is frequently expressed in DCIS, we used near-infrared fluorescently labeled CD44v6-targeting antibodies for detection of DCIS...
  53. ncbi Whole slide images for primary diagnostics of paediatric pathology specimens: a feasibility study
    Shaimaa Al-Janabi
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Pathol 66:218-23. 2013
    ..The aim of this study was to test the feasibility of using WSI in primary diagnostics of paediatric pathology specimens and placental tissue...
  54. doi Epigenetic progression of columnar cell lesions of the breast to invasive breast cancer
    Anoek H J Verschuur-Maes
    Department of Pathology, University Medical Center Utrecht Cancer Center, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Breast Cancer Res Treat 136:705-15. 2012
    ..Atypia in CCL or the presence of synchronous more advanced lesions does not seem to be accompanied by higher methylation levels...
  55. doi HIF-1α and NOTCH signaling in ductal and lobular carcinomas of the breast
    Cigdem Ercan
    Department of Pathology, University Medical Center Utrecht Cancer Center, Utrecht, The Netherlands
    Cell Oncol (Dordr) 35:435-42. 2012
    ..Hypoxia-induced stabilization of HIF transcription factors has been shown to lead to NOTCH activation in vitro in different contexts and tissues, causing differentiation arrest and induction of proliferation and migration...
  56. pmc Pathology of hereditary breast cancer
    Petra van der Groep
    Department of Pathology, University Medical Center Utrecht, P O Box 85500, 3508 GA, Utrecht, The Netherlands
    Cell Oncol (Dordr) 34:71-88. 2011
    ..However, in still a part of familial hereditary breast cancers no relationship to any of these breast cancer susceptibility genes can be found. Research on new susceptibility genes is therefore ongoing...
  57. ncbi Genomic profiling by array comparative genomic hybridization reveals novel DNA copy number changes in breast phyllodes tumours
    Arno Kuijper
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Cell Oncol 31:31-9. 2009
    ..Since copy number change was found in all benign PT, genomic instability may be an early event in PT genesis...
  58. ncbi Hypoxic regulation of metastasis via hypoxia-inducible factors
    Eelke H Gort
    Department of Pathology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Curr Mol Med 8:60-7. 2008
    ..This polyclonal hypoxic gene profile potentially develops into a metastatic profile, driving metastasis formation. The hypoxic gene profile in primary tumors may therefore provide a prognostic indicator in clinical decision-making...
  59. doi v-Raf murine sarcoma viral oncogene mutation status in serous borderline ovarian tumors and the effect on clinical behavior
    Marjolijn B Verbruggen
    Department of Obstetrics and Gynaecology, VU University Medical Center, Amsterdam, The Netherlands
    Int J Gynecol Cancer 19:1560-3. 2009
    ....
  60. pmc Expression of BNIP3 in invasive breast cancer: correlations with the hypoxic response and clinicopathological features
    Esther A Koop
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    BMC Cancer 9:175. 2009
    ..The aim of the present study was to investigate the expression of BNIP3 in invasive breast cancer at the mRNA and protein level in correlation with the hypoxic response and clinicopathological features...
  61. doi The prognostic and clinical value of morphometry and DNA cytometry in borderline ovarian tumors: a prospective study
    Marjolijn B Verbruggen
    Department of Obstetrics and Gynaecology, VU University Medical Center, Amsterdam, The Netherlands
    Int J Gynecol Pathol 28:35-40. 2009
    ..In contrast, histologic subtype and FIGO stage seem to be stable prognosticators in BOTs...
  62. doi Isolated tumor cells in breast cancer sentinel lymph nodes: displacement or metastases? An immunohistochemical study
    Carolien H M van Deurzen
    Department of Pathology, University Medical Centre Utrecht, The Netherlands
    Hum Pathol 40:778-82. 2009
    ..This supports the hypothesis that some of these cells may be displaced benign cells or concern tumor cells with limited malignant potential compared with micro- and macrometastases...
  63. ncbi HER-2/neu amplification testing in breast cancer by multiplex ligation-dependent probe amplification in comparison with immunohistochemistry and in situ hybridization
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Heidelberglaan 100, Utrecht, The Netherlands
    Cell Oncol 31:1-10. 2009
    ..This study aimed to validate a new PCR based test, called Multiplex Ligation-dependent Probe Amplification (MLPA), as a simple and quick method to assess HER-2/neu gene amplification status in invasive breast cancer...
  64. ncbi Cultures of ovarian surface epithelium from women with and without a hereditary predisposition to develop female adnexal carcinoma
    Jurgen M J Piek
    Department of Obstetrics and Gynaecology, VU University Medical Center, Amsterdam, The Netherlands
    Gynecol Oncol 92:819-26. 2004
    ..This study aims to detect differences in growth potential and morphology that are maintained or specifically expressed in vitro...
  65. doi No non-sentinel node involvement in melanoma patients with limited Breslow thickness and low sentinel node tumour load
    Thomas Bogenrieder
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Histopathology 59:318-26. 2011
    ..The aim of the present study was to predict the absence of non-SN metastases in a multicentre study of patients with a positive SN based on primary melanoma features and SN tumour load...
  66. doi Whole slide images as a platform for initial diagnostics in histopathology in a medium-sized routine laboratory
    Shaimaa Al-Janabi
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Pathol 65:1107-11. 2012
    ..This article is aimed to describe the gradual integration of WSI into routine pathology diagnostics in a medium-sized routine pathology laboratory...
  67. pmc p53 mutations in classic and pleomorphic invasive lobular carcinoma of the breast
    Cigdem Ercan
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cell Oncol (Dordr) 35:111-8. 2012
    ....
  68. doi Whole slide images for primary diagnostics in dermatopathology: a feasibility study
    Shaimaa Al-Janabi
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Pathol 65:152-8. 2012
    ..However, WSI have not regularly been used for routine diagnosis, because of the lack of validation studies...
  69. pmc Successful oxytocin-assisted nipple aspiration in women at increased risk for breast cancer
    Karijn P M Suijkerbuijk
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA, Utrecht, The Netherlands
    Fam Cancer 9:321-5. 2010
    ..6 (on a 0-10 scale), which was significantly lower than for mammography or MRI. These findings suggest that a new tool for biomarker detection in oxytocin-assisted nipple fluid of women at high risk for breast cancer is at hand...
  70. ncbi Local administration of granulocyte/macrophage colony-stimulating factor increases the number and activation state of dendritic cells in the sentinel lymph node of early-stage melanoma
    Ronald J C L M Vuylsteke
    Department of Surgical Oncology, VU University Medical Center, Amsterdam, The Netherlands
    Cancer Res 64:8456-60. 2004
    ....
  71. ncbi Molecular analysis of nipple fluid for breast cancer screening
    Karijn P M Suijkerbuijk
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Pathobiology 75:149-52. 2008
    ..The application of genomic and proteomic diagnostics to nipple aspirates therefore provides unprecedented opportunities for early breast cancer diagnosis amendable to population-based screening...
  72. pmc Expression of hypoxia-inducible factor 1 alpha and its downstream targets in fibroepithelial tumors of the breast
    Arno Kuijper
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Breast Cancer Res 7:R808-18. 2005
    ..We studied the expression and prognostic relevance of HIF-1alpha and its downstream targets in phyllodes tumors and fibroadenomas of the breast...
  73. pmc Low frequency of HER2 amplification and overexpression in early onset gastric cancer
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Heidelberglaan 100, PO Box 85500, 3508GA, Utrecht, The Netherlands
    Cell Oncol (Dordr) 34:89-95. 2011
    ..In light of potential trastuzumab benefit, subpopulations of GC such as EOGC (versus late onset) should be evaluated for their frequency of amplification and overexpression using currently available techniques...
  74. pmc HER-2/neu amplification testing in breast cancer by Multiplex Ligation-dependent Probe Amplification: influence of manual- and laser microdissection
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Heidelberglaan, GA Utrecht, The Netherlands
    BMC Cancer 9:4. 2009
    ..MLPA was previously shown to correlate well with IHC and in situ hybridization (ISH), but a low tumor percentage in the tissue tested could negatively affect the accuracy of MLPA results...
  75. ncbi Levels of hypoxia-inducible factor-1alpha independently predict prognosis in patients with lymph node negative breast carcinoma
    Reinhard Bos
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Cancer 97:1573-81. 2003
    ..In this study, the prognostic value of HIF-1alpha expression and its correlation with various clinicopathologic variables in patients with invasive breast carcinoma were investigated...
  76. ncbi FANCD2 protein is expressed in proliferating cells of human tissues that are cancer-prone in Fanconi anaemia
    Michael Hölzel
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    J Pathol 201:198-203. 2003
    ..FANCD2 expression is frequently expressed in proliferating cells as demonstrated by Ki-67 immunofluorescence double staining, consistent with a function of FANCD2 in DNA replication...
  77. doi Comparison of different promoter methylation assays in breast cancer
    Karijn P M Suijkerbuijk
    Department of Pathology, University Medical Center Utrecht Cancer Center, Utrecht, The Netherlands
    Anal Cell Pathol (Amst) 33:133-41. 2010
    ..Here we directly compared three commonly used methylation assays and assessed the influence of tissue fixation, target sequence location and the amount of DNA on their performance...
  78. ncbi Confocal 3D DNA cytometry: assessment of required coefficient of variation by computer simulation
    Lennert S Ploeger
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cell Oncol 26:93-9. 2004
    ..The aim of this study was to analyze the required CV for 3D CLSM DNA histograms given a realistic sample size...
  79. doi Formaldehyde substitute fixatives: effects on nucleic acid preservation
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Pathol 64:960-7. 2011
    ..However, the commonly used formalin fixative is carcinogenic, and its crosslinking impairs DNA and RNA quality...
  80. doi Molecular differences between ductal carcinoma in situ and adjacent invasive breast carcinoma: a multiplex ligation-dependent probe amplification study
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Anal Cell Pathol (Amst) 33:165-73. 2010
    ..These high-grade DCIS specific genes may be potential targets for treatment and/or predict progression...
  81. ncbi A restaining method to restore faded fluorescence in tissue specimens for quantitative confocal microscopy
    André Huisman
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Cytometry A 71:875-81. 2007
    ..With the described protocol it is possible to remove TO-PRO-3 fluorescence from tissue sections that can successfully be restained with minimal influence on fluorescence intensity and nuclear chromatin distribution...
  82. pmc The microanatomic location of metastatic breast cancer in sentinel lymph nodes predicts nonsentinel lymph node involvement
    Carolien H M van Deurzen
    Department of Pathology, St Antonius Hospital, Nieuwegein, The Netherlands
    Ann Surg Oncol 15:1309-15. 2008
    ..The present study was designed to assess whether these criteria might also be used to be more restrictive in selecting breast cancer patients who would benefit from an ALND...
  83. ncbi The prognostic value of proliferation in lymph-node-negative breast cancer patients is age dependent
    Jan P A Baak
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands, and Department of Pathology, Stavanger University Hospital, P O Box 8100, 4068 Stavanger, Norway
    Eur J Cancer 43:527-35. 2007
    ..006) for patients aged 55-70 years, while over 70 years, MAI was not significant (P=.11). The prognostic value of proliferation was age-dependent. Prognostic breast cancer studies must clearly indicate the age group being studied...
  84. ncbi Ovarian carcinogenesis: an alternative hypothesis
    Jurgen M J Piek
    Department of Obstetrics and Gynaecology, VU University Medical Center, Amsterdam, The Netherlands
    Adv Exp Med Biol 622:79-87. 2008
    ....
  85. ncbi p300 and p53 levels determine activation of HIF-1 downstream targets in invasive breast cancer
    Marije M Vleugel
    Department of Pathology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Hum Pathol 37:1085-92. 2006
    ..This underlines the importance of p300 levels and p53 accumulation in the HIF-1-regulated response toward hypoxia...
  86. ncbi FANCD2 expression in advanced non-small-cell lung cancer and response to platinum-based chemotherapy
    Miriam Ferrer
    Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands
    Clin Lung Cancer 6:250-4. 2005
    ..In conclusion, the activation status of the FA pathway had no value in predicting sensitivity to platinum-based chemotherapy in patients with advanced NSCLC...
  87. ncbi Confocal DNA cytometry: a contour-based segmentation algorithm for automated three-dimensional image segmentation
    Jeroen A M Belien
    Department of Pathology, VU Medical Center, Amsterdam, The Netherlands
    Cytometry 49:12-21. 2002
    ..So far, these measurements have been performed manually, which is quite time-consuming...
  88. ncbi Prognostic factors in adult soft tissue sarcomas of the head and neck: a single-centre experience
    Remco de Bree
    Department of Otolaryngology Head and Neck Surgery, Vrije Universiteit University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands
    Oral Oncol 42:703-9. 2006
    ..Negative surgical margins are an important prognostic factor, but are difficult to obtain in head and neck sarcomas...
  89. pmc The correlation between clinical, nuclear and histologic findings in a patient with Von Recklinghausen's disease
    Justus Martijn Brinkman
    Department of Nuclear Medicine and PET Research, VU University Medical Center, Amsterdam, The Netherlands
    World J Surg Oncol 5:130. 2007
    ..abstract:..
  90. ncbi Biologic correlates of (18)fluorodeoxyglucose uptake in human breast cancer measured by positron emission tomography
    Reinhard Bos
    Department of Pathology, Positron Emission Tomography Research Center, Vrije Universiteit Medical Center, Amsterdam, The Netherlands
    J Clin Oncol 20:379-87. 2002
    ..At present, no satisfactory biologic explanation exists for this phenomenon. This study compared (18)FDG uptake in vivo with biomarkers expected to be involved in the underlying biologic mechanisms...
  91. ncbi Laparoscopic detection of sentinel lymph nodes followed by lymph node dissection in patients with early stage cervical cancer
    Marrije R Buist
    Department of Obstetrics, Nuclear Medicine, and Pathology, VU Medical Center, P O Box 7057, 1007 MB Amsterdam, The Netherlands
    Gynecol Oncol 90:290-6. 2003
    ..Furthermore, the results of laparoscopic pelvic lymph node dissection were studied, validated by subsequent laparotomy...
  92. ncbi Pattern and incidence of first site recurrences following sentinel node procedure in melanoma patients
    Markwin G Statius Muller
    Department of Surgical Oncology, VU University Medical Centre, PO Box 7057, 1007 MB Amsterdam, The Netherlands
    World J Surg 26:1405-11. 2002
    ..High Breslow thickness, lymphatic invasion, and ulceration of the primary melanoma are strong risk factors for recurrence...
  93. ncbi Bronchial typical carcinoid tumors: histological study of intra- versus extraluminal growth pattern
    Hes A P Brokx
    Department of Pulmonology, Vrije Universiteit Medical Center, PO Box 7057, Amsterdam 1007 MB, The Netherlands
    Lung Cancer 44:129-30. 2004
  94. ncbi Analysis of the progression of fibroepithelial tumours of the breast by PCR-based clonality assay
    Arno Kuijper
    Department of Pathology, VU University Medical Centre, Amsterdam, The Netherlands
    J Pathol 197:575-81. 2002
    ..These findings indicate that fibroadenoma can progress in an epithelial direction to CIS and in a stromal direction to phyllodes tumour...
  95. doi Creation of a fully digital pathology slide archive by high-volume tissue slide scanning
    André Huisman
    Department of Pathology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Hum Pathol 41:751-7. 2010
    ..To give insight in the performance of our digital archive, we provide some statistics as well. We also present our thoughts on future developments in the area of digital slide scanning...
  96. ncbi Comparative molecular and histological grading of epithelial dysplasia of the oral cavity and the oropharynx
    Maarten P Tabor
    Department of Otolaryngology Head and Neck Surgery, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands
    J Pathol 199:354-60. 2003
    ..Assessment of proliferation with Ki-67 is a better surrogate for LOH than histological grading...
  97. ncbi Histopathological characteristics of BRCA1- and BRCA2-associated intraperitoneal cancer: a clinic-based study
    Jurgen M J Piek
    Departments of Obstetrics and Gynaecology, VU University Medical Center, Amsterdam, The Netherlands
    Fam Cancer 2:73-8. 2003
    ..This suggests, therefore, that serous tumors may be the only subtype related to a BRCA1 or BRCA2 mutation. Furthermore, fallopian tube carcinoma occurred more often in BRCA mutation carriers than in the reference population...
  98. ncbi Serous borderline tumor of the ovary presenting with cervical lymph node involvement: a report of 3 cases
    Marjolijn B Verbruggen
    Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, VU University Medical Center, and Department of Gynecology, Dutch Cancer Institute, Amsterdam, The Netherlands
    Am J Surg Pathol 30:739-43. 2006
    ..When fine needle aspiration cytology of such a lymph node is compatible with adenocarcinoma of unknown primary, serous BOT should be included in the differential diagnosis and pelvic examination should be performed...
  99. doi Validation of a fully automated HER2 staining kit in breast cancer
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Cell Oncol 32:149-55. 2010
    ..Analytical variability in HER2 IHC testing could be diminished by a fully automatic staining system with a monoclonal antibody...
  100. ncbi Expression of multidrug resistance-associated markers, their relation to quantitative pathologic tumour characteristics and prognosis in advanced ovarian cancer
    Mariël Brinkhuis
    Department of Pathology, Free University Medical Center, Amsterdam, The Netherlands
    Anal Cell Pathol 24:17-23. 2002
    ..Further studies are required to elucidate the biological background of the strong prognostic value of mean nuclear area in advanced ovarian cancer...
  101. ncbi Atypical MEN type 2B associated with two germline RET mutations on the same allele not involving codon 918
    Fred H Menko
    Department of Clinical Genetics and Human Genetics, Vrije Universiteit Medical Center, 1007 MB Amsterdam, The Netherlands
    J Clin Endocrinol Metab 87:393-7. 2002
    ..Mutation analysis revealed a double RET germline mutation, Val804Met and Ser904Cys, in affected individuals. The clinical phenotype, the functional effect of the mutations, and the clinical implications of our findings are discussed...