Jeroen Kool

Summary

Affiliation: Vrije Universiteit
Country: The Netherlands

Publications

  1. ncbi request reprint High throughput screening methodologies classified for major drug target classes according to target signaling pathways
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Science, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, Amsterdam, The Netherlands
    Comb Chem High Throughput Screen 13:548-61. 2010
  2. pmc Advances in mass spectrometry-based post-column bioaffinity profiling of mixtures
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, The Netherlands
    Anal Bioanal Chem 399:2655-68. 2011
  3. doi request reprint High-resolution bioactivity profiling of mixtures toward the acetylcholine binding protein using a nanofractionation spotter technology
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, Amsterdam, The Netherlands
    J Biomol Screen 16:917-24. 2011
  4. doi request reprint Nanofractionation spotter technology for rapid contactless and high-resolution deposition of LC eluent for further off-line analysis
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, and FMI Bèta VU, ELE Bèta VU Mechanical and Electronic Engineering, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081HV Amsterdam, The Netherlands
    Anal Chem 83:125-32. 2011
  5. doi request reprint Online fluorescence enhancement assay for the acetylcholine binding protein with parallel mass spectrometric identification
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081HV Amsterdam, The Netherlands
    J Med Chem 53:4720-30. 2010
  6. pmc Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays
    Ferry Heus
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    Anal Bioanal Chem 398:3023-32. 2010
  7. ncbi request reprint Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures
    Jeroen Kool
    LACDR Division of Molecular Toxicology, Department of Pharmacochemistry, Vrije Universiteit, Amsterdam, The Netherlands
    J Biomol Screen 12:396-405. 2007
  8. ncbi request reprint Development of three parallel cytochrome P450 enzyme affinity detection systems coupled on-line to gradient high-performance liquid chromatography
    Jeroen Kool
    Vrije Universiteit, Department of Pharmacochemistry, LACDR Division of Molecular Toxicology, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    Drug Metab Dispos 35:640-8. 2007
  9. pmc Targeted LC-MS derivatization for aldehydes and carboxylic acids with a new derivatization agent 4-APEBA
    Mark Eggink
    Section of Biomolecular Analysis, Faculty of Science, VU University Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands
    Anal Bioanal Chem 397:665-75. 2010
  10. pmc On-line electrochemistry-bioaffinity screening with parallel HR-LC-MS for the generation and characterization of modified p38α kinase inhibitors
    David Falck
    Division of BioMolecular Analysis, Faculty of Sciences, VU University Amsterdam, Amsterdam, The Netherlands
    Anal Bioanal Chem 403:367-75. 2012

Collaborators

Detail Information

Publications38

  1. ncbi request reprint High throughput screening methodologies classified for major drug target classes according to target signaling pathways
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Science, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, Amsterdam, The Netherlands
    Comb Chem High Throughput Screen 13:548-61. 2010
    ..Furthermore, special attention is paid to less traditional (non-platereader based) state-of-the-art screening approaches such as LC-MS, microarrays, microfluidics and sensor based technologies...
  2. pmc Advances in mass spectrometry-based post-column bioaffinity profiling of mixtures
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, The Netherlands
    Anal Bioanal Chem 399:2655-68. 2011
    ..Furthermore, off-line screening is discussed briefly with the on-line and at-line approaches...
  3. doi request reprint High-resolution bioactivity profiling of mixtures toward the acetylcholine binding protein using a nanofractionation spotter technology
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, Amsterdam, The Netherlands
    J Biomol Screen 16:917-24. 2011
    ....
  4. doi request reprint Nanofractionation spotter technology for rapid contactless and high-resolution deposition of LC eluent for further off-line analysis
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, and FMI Bèta VU, ELE Bèta VU Mechanical and Electronic Engineering, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081HV Amsterdam, The Netherlands
    Anal Chem 83:125-32. 2011
    ....
  5. doi request reprint Online fluorescence enhancement assay for the acetylcholine binding protein with parallel mass spectrometric identification
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081HV Amsterdam, The Netherlands
    J Med Chem 53:4720-30. 2010
    ..This led to the possibility of affinity ranking of ligands in mixtures with parallel high-resolution mass spectrometry for compound identification...
  6. pmc Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays
    Ferry Heus
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    Anal Bioanal Chem 398:3023-32. 2010
    ..Finally, coupling of the detection system to gradient reversed-phase nano-LC allowed analysis of mixtures in order to identify the bioactive compounds present by injecting 10 nL of each mixture...
  7. ncbi request reprint Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures
    Jeroen Kool
    LACDR Division of Molecular Toxicology, Department of Pharmacochemistry, Vrije Universiteit, Amsterdam, The Netherlands
    J Biomol Screen 12:396-405. 2007
    ..It is concluded that the present online gradient HPLC-based HRS screening technology offers new perspectives for sensitive and simultaneous screening of general cGSTs and specific GST P1 inhibitors in mixtures...
  8. ncbi request reprint Development of three parallel cytochrome P450 enzyme affinity detection systems coupled on-line to gradient high-performance liquid chromatography
    Jeroen Kool
    Vrije Universiteit, Department of Pharmacochemistry, LACDR Division of Molecular Toxicology, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    Drug Metab Dispos 35:640-8. 2007
    ..The triple parallelized P450-EAD system was shown to enable rapid profiling of individual components in complex mixtures for inhibitory activity to three different P450s...
  9. pmc Targeted LC-MS derivatization for aldehydes and carboxylic acids with a new derivatization agent 4-APEBA
    Mark Eggink
    Section of Biomolecular Analysis, Faculty of Science, VU University Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands
    Anal Bioanal Chem 397:665-75. 2010
    ....
  10. pmc On-line electrochemistry-bioaffinity screening with parallel HR-LC-MS for the generation and characterization of modified p38α kinase inhibitors
    David Falck
    Division of BioMolecular Analysis, Faculty of Sciences, VU University Amsterdam, Amsterdam, The Netherlands
    Anal Bioanal Chem 403:367-75. 2012
    ..Both products were detected in the p38α bioaffinity assay, which indicates binding to the p38α kinase...
  11. ncbi request reprint Rapid on-line profiling of estrogen receptor binding metabolites of tamoxifen
    Jeroen Kool
    Leiden Amsterdam Center for Drug Research Division of Molecular Toxicology, Department of Chemistry and Pharmacochemistry, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    J Med Chem 49:3287-92. 2006
    ..Most of the 14 metabolites detected exhibited affinity for the ERalpha. The HRS methodology shows great potential for metabolite bio-affinity profiling and application in drug discovery and development...
  12. pmc Reversed-phase liquid chromatography coupled on-line to estrogen receptor bioaffinity detection based on fluorescence polarization
    Jelle Reinen
    Department of Chemistry and Pharmaceutical Sciences, LACDR Division of Molecular Toxicology, Vrije Universiteit, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands
    Anal Bioanal Chem 390:1987-98. 2008
    ..It is concluded that the on-line FP-based bioassay can be used to screen for the affinity of compounds without the disturbing occurrence of autofluorescence...
  13. doi request reprint Determination and identification of estrogenic compounds generated with biosynthetic enzymes using hyphenated screening assays, high resolution mass spectrometry and off-line NMR
    Jon S B de Vlieger
    LACDR Division of Biomolecular Analysis, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    J Chromatogr B Analyt Technol Biomed Life Sci 878:667-74. 2010
    ..g. metabolism studies and compound-library screening...
  14. doi request reprint Screening of protein-ligand interactions using dynamic protein-affinity chromatography solid-phase extraction-liquid chromatography-mass spectrometry
    Niels Jonker
    VU University Amsterdam, Faculty of Sciences, Department of Chemistry, Biomolecular Analysis Group, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    J Chromatogr A 1205:71-7. 2008
    ..The weakest binder that can be identified is norethindrone (approximately K(d)=0.1-1mM). The same setup also provides the possibilities to measure EC50 curves of both weak and strong binders...
  15. ncbi request reprint Cytochrome P450 bio-affinity detection coupled to gradient HPLC: on-line screening of affinities to cytochrome P4501A2 and 2D6
    Jeroen Kool
    LACDR Division of Molecular Toxicology, Department of Pharmacochemistry, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    J Chromatogr B Analyt Technol Biomed Life Sci 858:49-58. 2007
    ..Finally, the on-line CYP2D6 EAD system was used to screen for the inhibitory activities of stereoisomers of a mixture of five methylenedioxy-alkylamphetamines (XTC analogs) on a chiral analytical column...
  16. doi request reprint Online magnetic bead based dynamic protein affinity selection coupled to LC-MS for the screening of acetylcholine binding protein ligands
    Lionel Pochet
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081HV Amsterdam, The Netherlands
    J Chromatogr B Analyt Technol Biomed Life Sci 879:1781-8. 2011
    ..The results obtained with AChBP as target protein demonstrated reliable discrimination between binders with pK(i) values ranging from at least 6.26 to 8.46 and non-binders...
  17. doi request reprint High temperature liquid chromatography hyphenated with ESI-MS and ICP-MS detection for the structural characterization and quantification of halogen containing drug metabolites
    Jon S B de Vlieger
    Biomolecular Analysis Group, Faculty of Sciences, VU University Amsterdam, The Netherlands
    Anal Chim Acta 698:69-76. 2011
    ..The importance of these kinds of quantification strategies is stressed by the observation that the difference of the position of one oxygen atom in a structure can greatly affect its response in ESI-MS and UV detection...
  18. ncbi request reprint Metabolic profiling of endocrine-disrupting compounds by on-line cytochrome p450 bioreaction coupled to on-line receptor affinity screening
    Sebastiaan M van Liempd
    LACDR Division of Molecular Toxicology, Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit, Amsterdam, The Netherlands
    Chem Res Toxicol 20:1825-32. 2007
    ..The hyphenated P450-bioreactor-SPE-HPLC-HRS-ERAD methodology presented here will be of great interest in on-line research of metabolic activation of endocrine-disrupting compounds...
  19. doi request reprint Development of surface plasmon resonance biosensor assays for primary and secondary screening of acetylcholine binding protein ligands
    Kim Retra
    Leiden Amsterdam Center for Drug Research, Division of BioMolecular Analysis, Faculty of Sciences, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
    Anal Biochem 407:58-64. 2010
    ..96. Using both assays, we identified the compound tacrine as a ligand for AChBP...
  20. doi request reprint Development of an on-line weak-cation exchange liquid chromatography-tandem mass spectrometric method for screening aldehyde products in biological matrices
    Mark Eggink
    VU University Amsterdam, Faculty of Science, Section of Analytical Chemistry and Applied Spectroscopy, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    J Chromatogr B Analyt Technol Biomed Life Sci 877:3937-45. 2009
    ..An in vitro model shows how derivatized aldehydes in human and rat plasma are detected. Finally, plasma treated with radical inducer shows elevated aldehyde species compared to untreated plasma...
  21. pmc An on-line post-column detection system for the detection of reactive-oxygen-species-producing compounds and antioxidants in mixtures
    Jeroen Kool
    LACDR Division of Molecular Toxicology, Department of Pharmacochemistry, Vrije Universiteit, Amsterdam, The Netherlands
    Anal Bioanal Chem 388:871-9. 2007
    ..The resulting high-resolution screening technology was able to separate mixtures of ROS-producing compounds and antioxidants, allowing each species to be characterized rapidly and sensitively...
  22. pmc Recent developments in protein-ligand affinity mass spectrometry
    Niels Jonker
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, The Netherlands
    Anal Bioanal Chem 399:2669-81. 2011
    ..The review addresses the principles of each of the methods discussed, with a focus on developments in recent years, and the applicability of the methods to lead generation and development in drug discovery...
  23. pmc Development of an online p38α mitogen-activated protein kinase binding assay and integration of LC-HR-MS
    David Falck
    LACDR Division of Biomolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, VU University Amsterdam, 1081 HV Amsterdam, The Netherlands
    Anal Bioanal Chem 398:1771-80. 2010
    ..8, which is suitable for semi-quantitative affinity measurements and applicable to various binding modes. Furthermore, the integrated approach gives affinity data on individual compounds instead of averaged ones for mixtures...
  24. doi request reprint Development of a selective ESI-MS derivatization reagent: synthesis and optimization for the analysis of aldehydes in biological mixtures
    Mark Eggink
    Leiden Amsterdam Center for Drug Research, Faculty of Sciences, Department of Chemistry, Section of Analytical Chemistry and Applied Spectroscopy, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    Anal Chem 80:9042-51. 2008
    ..Finally, of all aldehydes, pentanal and hexanal were detected at elevated levels in pooled healthy human urine samples...
  25. doi request reprint Microfractionation revisited: a 1536 well high resolution screening assay
    Martin Giera
    Biomolecular Analysis Group, Department of Chemistry, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    Anal Chem 81:5460-6. 2009
    ..Furthermore, it allows coupling of a microbore HPLC with a biochemical screening assay without compromising resolution and overcomes problems associated with the 1536 well format...
  26. doi request reprint Production and on-line acetylcholinesterase bioactivity profiling of chemical and biological degradation products of tacrine
    Lygia Azevedo Marques
    VU University Amsterdam, Faculty of Sciences, Section of Biomolecular Analysis, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    J Pharm Biomed Anal 53:609-16. 2010
    ..Furthermore, chemical degradation can be used to produce conveniently and in relatively large quantities clean mixtures of compounds that are also produced during metabolic incubations...
  27. doi request reprint Analytical workflow for rapid screening and purification of bioactives from venom proteomes
    Reka A Otvos
    AIMMS Division of BioMolecular Analysis, Faculty of Sciences, De Boelelaan 1081, 1083 HV Amsterdam, Amsterdam VU University, The Netherlands Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, VU University Amsterdam, The Netherlands Electronic address
    Toxicon 76:270-81. 2013
    ..These peptides were not reported before to have affinity for the AChBP. The purified peptides can be used for further biological studies...
  28. doi request reprint Development of a profiling strategy for metabolic mixtures by combining chromatography and mass spectrometry with cell-based GPCR signaling
    Saskia Nijmeijer
    Medicinal Chemistry, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University, Amsterdam, The Netherlands
    J Biomol Screen 17:1329-38. 2012
    ....
  29. doi request reprint An efficient analytical platform for on-line microfluidic profiling of neuroactive snake venoms towards nicotinic receptor affinity
    Ferry Heus
    AIMMS Division of BioMolecular Analysis, Faculty of Sciences, De Boelelaan 1083, 1081 HV Amsterdam, VU University Amsterdam, The Netherlands
    Toxicon 61:112-24. 2013
    ..Our methodology has the potential to aid the field of profiling complex animal venoms for drug discovery...
  30. pmc Studying protein-protein affinity and immobilized ligand-protein affinity interactions using MS-based methods
    Jeroen Kool
    BioMolecular Analysis, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, Amsterdam, The Netherlands
    Anal Bioanal Chem 401:1109-25. 2011
    ..The review focuses on the analysis of bioaffinity interactions of proteins with other proteins and with ligands, where the proteins are considered as the bioactives analyzed by MS...
  31. doi request reprint Comparison of (bio-)transformation methods for the generation of metabolite-like compound libraries of p38α MAP kinase inhibitors using high-resolution screening
    David Falck
    AIMMS Division of BioMolecular Analysis, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    J Pharm Biomed Anal 88:235-44. 2014
    ..In this context, HRS is demonstrated to be an effective tool as it reduces the effort directed towards laborious synthesis and purification schemes. ..
  32. ncbi request reprint A flow-through fluorescence polarization detection system for measuring GPCR-mediated modulation of cAMP production
    Jeroen Kool
    LACDR Division of Molecular Toxicology, Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam, The Netherlands
    J Biomol Screen 12:1074-83. 2007
    ....
  33. doi request reprint Online screening of homogeneous catalyst performance using reaction detection mass spectrometry
    Cornelius T Martha
    Department of Analytical Chemistry and Applied Spectroscopy, Faculty of Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    Anal Chem 80:7121-7. 2008
    ....
  34. ncbi request reprint Development of a novel cytochrome p450 bioaffinity detection system coupled online to gradient reversed-phase high-performance liquid chromatography
    Jeroen Kool
    LACDR Division of Molecular Toxicology, Department of Pharmacochemistry, Vrije Universiteit, Amsterdam, The Netherlands
    J Biomol Screen 10:427-36. 2005
    ..It is concluded that this novel screening technology offers new perspectives for rapid and sensitive screening of individual compounds in mixtures exhibiting affinity for liver microsomal Cyt P450s...
  35. doi request reprint A high-throughput sample preparation method for cellular proteomics using 96-well filter plates
    Linda Switzar
    AIMMS Division of BioMolecular Analysis, VU University, Amsterdam, The Netherlands
    Proteomics 13:2980-3. 2013
    ..The optimized protocol allowed for higher throughput with improved sensitivity in terms of the number of identified cellular proteins when compared to an established protocol employing gel-filtration columns...
  36. doi request reprint High-resolution fractionation after gas chromatography for effect-directed analysis
    Eelco Pieke
    AIMMS Division of BioAnalytical Chemistry, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    Anal Chem 85:8204-11. 2013
    ....
  37. ncbi request reprint The chemical interaction between the estrogen receptor and monohydroxybenzo[a]pyrene derivatives studied by fluorescence line-narrowing spectroscopy
    Arjen N Bader
    Department of Analytical Chemistry and Applied Spectroscopy, Laser Centre, Amsterdam, The Netherlands
    Chem Res Toxicol 18:1405-12. 2005
    ..Furthermore, the spectra indicated a pi-pi stacking aromatic interaction for 9-OH-BaP with PHE404. These results are in line with molecular modeling studies published earlier...
  38. ncbi request reprint Development and validation of a microsomal online cytochrome P450 bioreactor coupled to solid-phase extraction and reversed-phase liquid chromatography
    Sebastiaan M van Liempd
    LACDR Division of Molecular Toxicology, Department of Pharmacochemistry, Vrije Universiteit, De Boelelaan 1083, 1081 HVAmsterdam, The Netherlands
    J Chromatogr A 1075:205-12. 2005
    ..66 +/- 0.08 microM and 6.4 +/- 0.2 nmol/min/mg protein respectively. In conclusion the newly developed analytical method can be used effectively and at a microliter scale for online generation, extraction and separation of metabolites...