Research Topics
| Marie Louise GrootSummaryAffiliation: Vrije Universiteit Country: The Netherlands Publications
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Detail Information
Publications
Initial electron donor and acceptor in isolated Photosystem II reaction centers identified with femtosecond mid-IR spectroscopyMarie Louise Groot
Faculty of Sciences, Vrije Universiteit, 1081 HV, Amsterdam, The Netherlands
Proc Natl Acad Sci U S A 102:13087-92. 2005..We conclude therefore that in the Photosystem II RC the primary charge separation occurs between the "accessory chlorophyll" Chl(D1) and the pheophytin on the so-called active branch...
Time-resolved methods in biophysics. 5. Femtosecond time-resolved and dispersed infrared spectroscopy on proteinsMarie Louise Groot
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands
Photochem Photobiol Sci 6:501-7. 2007..We further discuss a few examples of experiments performed on the photoactive yellow protein and photosynthetic complexes in more detail...- Hydrogen bond switching among flavin and amino acid side chains in the BLUF photoreceptor observed by ultrafast infrared spectroscopyCosimo Bonetti
Biophysics Group, Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, Amsterdam, The Netherlands
Biophys J 95:4790-802. 2008..The results support a previously hypothesized rotation of glutamine by approximately 180 degrees through a light-driven radical-pair mechanism as the determinant of the hydrogen-bond switch... 
Conformational changes in an ultrafast light-driven enzyme determine catalytic activityOlga A Sytina
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
Nature 456:1001-4. 2008....- Identification of the first steps in charge separation in bacterial photosynthetic reaction centers of Rhodobacter sphaeroides by ultrafast mid-infrared spectroscopy: electron transfer and protein dynamicsNatalia P Pawlowicz
Faculty of Sciences, Department of Physics and Astronomy, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Biophys J 95:1268-84. 2008.... - A femtosecond visible/visible and visible/mid-infrared transient absorption study of the light harvesting complex IIAndreas D Stahl
Faculty of Sciences, Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, The Netherlands
Biophys J 97:3215-23. 2009..We find that information derived from mid-infrared spectra, together with structural and modeling information, provides a unique visualization of the flow of energy via the bottleneck pigment chlorophyll a604... - Identification of excited-state energy transfer and relaxation pathways in the peridinin-chlorophyll complex: an ultrafast mid-infrared studyCosimo Bonetti
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands
Phys Chem Chem Phys 12:9256-66. 2010..Our results are consistent with an energy transfer scheme where the ICT state mainly localizes on Per621/611 and Per623/613, the S(1) state on Per622/612 and the triplet state on Per624/614... - Ultrafast spectroscopy of biological photoreceptorsJohn T M Kennis
Department of Biophysics, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands
Curr Opin Struct Biol 17:623-30. 2007..Furthermore, isomerization reactions of phycocyanobilin in phytochrome and retinal in the rhodopsins have been revealed in detail through application of femtosecond infrared and femtosecond-stimulated Raman spectroscopy... - Triplet state dynamics in peridinin-chlorophyll-a-protein: a new pathway of photoprotection in LHCs?Maxime T A Alexandre
Department of Biophysics and Physics of Complex Systems, Division of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
Biophys J 93:2118-28. 2007..We suggest that this Per-Chl-a interaction via a delocalized triplet state lowers the (3)Per energy and thus provides a general, photoprotection mechanism for light-harvesting antenna complexes... - Primary reactions of the LOV2 domain of phototropin studied with ultrafast mid-infrared spectroscopy and quantum chemistryMaxime T A Alexandre
Biophysics Group, Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, Amsterdam, The Netherlands
Biophys J 97:227-37. 2009..Finally, we observed a heterogeneous population of singly and doubly H-bonded FMN C(4)=O conformers in the dark state, with stretch frequencies at 1714 cm(-1) and 1694 cm(-1), respectively... - Enzyme activation and catalysis: characterisation of the vibrational modes of substrate and product in protochlorophyllide oxidoreductaseOlga A Sytina
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
Phys Chem Chem Phys 13:2307-13. 2011..The FTIR spectra of the enzyme activation phase indicate involvement of a nucleotide-binding structural motif, and an increased exposure of the protein to solvent after activation... - Proton transfer events in GFPMariangela Di Donato
Department of Physics and Astronomy, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
Phys Chem Chem Phys 13:16295-305. 2011..This state is followed by a repositioning of the protons on the wire in 10 ps (80 ps in D(2)O), ultimately forming the fully deprotonated chromophore and protonated Glu222... - Reaction pathways of photoexcited retinal in proteorhodopsin studied by pump-dump-probe spectroscopyAlisa Rupenyan
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
J Phys Chem B 113:16251-6. 2009..Inclusion of this intermediate in the kinetic scheme led to more consistent spectra of the retinal-excited state, and to a more accurate estimation of the quantum yield of isomerization (Phi = 0.4 at pH 6)... - Spectroscopic characterization of the first ultrafast catalytic intermediate in protochlorophyllide oxidoreductaseOlga A Sytina
Department of Physics and Astronomy, Faculty of Sciences, VU University, Amsterdam, The Netherlands
Phys Chem Chem Phys 14:616-25. 2012..64 ± 0.11, and the quantum yield of the final reaction product formed on a later time scale, chlorophyllide, is 0.26 ± 0.06. Several possible interpretations of these data are discussed... - On the involvement of single-bond rotation in the primary photochemistry of photoactive yellow proteinAndreas D Stahl
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Biophys J 101:1184-92. 2011.... - Dynamics of carbon monoxide photodissociation in Bradyrhizobium japonicum FixL probed by picosecond midinfrared spectroscopyLuuk J G W van Wilderen
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, Amsterdam, The Netherlands
J Phys Chem B 113:3292-7. 2009..Srajer, V.; Pahl, R.; Moffat, K. Biochemistry 2007, 46, 4706)... - Cofactors involved in light-driven charge separation in photosystem I identified by subpicosecond infrared spectroscopyMariangela Di Donato
Faculty of Sciences, Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, The Netherlands
Biochemistry 50:480-90. 2011..radical pair... - Role of PufX in photochemical charge separation in the RC-LH1 complex from Rhodobacter sphaeroides: an ultrafast mid-IR pump-probe InvestigationAndreas D Stahl
Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, Amsterdam, The Netherlands
J Phys Chem B 116:434-44. 2012.... - CO photodissociation dynamics in cytochrome P450BM3 studied by subpicosecond visible and mid-infrared spectroscopyAlisa Rupenyan
Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, Amsterdam, The Netherlands
Biochemistry 48:6104-10. 2009..The bands partially decay with a time constant of 1 ps, possibly due to a relaxation of the protein around the CO docking site, allowing for greater orientational freedom of the CO molecules... - Proline 68 enhances photoisomerization yield in photoactive yellow proteinAlisa B Rupenyan
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, Amsterdam, The Netherlands
J Phys Chem B 115:6668-77. 2011..This study provides a molecular basis for the structural optimization of biological light sensing... - Characterization of the primary photochemistry of proteorhodopsin with femtosecond spectroscopyAlisa Rupenyan
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
Biophys J 94:4020-30. 2008..2 ps and 2 ps timescale and probably reflects a response of an amide II band in reaction to the isomerization process... - Ultrafast mid-infrared spectroscopy by chirped pulse upconversion in 1800-1000cm(-1) regionJingyi Zhu
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, Amsterdam, The Netherlands
Opt Express 20:10562-71. 2012..Possibilities for further optimization of the method are discussed. We conclude that this method is an attractive alternative for the traditional MCT arrays used in most mid-infrared pump probe experiments... - Modeling of multi-exciton transient absorption spectra of protochlorophyllide aggregates in aqueous solutionOlga A Sytina
Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, Amsterdam, The Netherlands
J Phys Chem A 115:11944-51. 2011..The relevance of our results to the optical properties and dynamics of natural photosynthetic complexes and the possible physical origin of CTS formation are discussed... - Excitation energy transfer in the photosystem II core antenna complex CP43 studied by femtosecond visible/visible and visible/mid-infrared pump probe spectroscopyMariangela Di Donato
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
J Phys Chem B 111:7345-52. 2007.... - Single and multi-exciton dynamics in aqueous protochlorophyllide aggregatesOlga A Sytina
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
J Phys Chem A 115:3936-46. 2011.... - Protochlorophyllide excited-state dynamics in organic solvents studied by time-resolved visible and mid-infrared spectroscopyOlga A Sytina
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
J Phys Chem B 114:4335-44. 2010..The consequences of these observations for the catalytic pathway and the role of the triplet and ICT state in activation of the enzyme are discussed... - Primary reactions of bacteriophytochrome observed with ultrafast mid-infrared spectroscopyK C Toh
Biophysics Group, Department of Physics and Astronomy, Faculty of Sciences, VU University, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands
J Phys Chem A 115:3778-86. 2011..Possible origins of this product band are discussed. This work may aid in rational structure- and mechanism-based conversion of BPh into an efficient near-IR fluorescent marker... - Primary charge separation in the photosystem II core from Synechocystis: a comparison of femtosecond visible/midinfrared pump-probe spectra of wild-type and two P680 mutantsMariangela Di Donato
Department of Physics, Free University Amsterdam, Amsterdam, The Netherlands
Biophys J 94:4783-95. 2008.... - Initial steps of signal generation in photoactive yellow protein revealed with femtosecond mid-infrared spectroscopyMarie Louise Groot
Faculty of Sciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
Biochemistry 42:10054-9. 2003..Relaxation of the coumaryl tail of the chromophore occurs in 0.9-1 ns, which event we identify with the I(0) to I(1) transition observed in visible spectroscopy... - Excited state proton transfer in strongly enhanced GFP (sGFP2)Bart van Oort
Institute for Lasers, Life and Biophotonics, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
Phys Chem Chem Phys 14:8852-8. 2012..We used pump-dump-probe spectroscopy in combination with target analysis to probe suitability of the sGFP2 fluorophore for super-resolution microscopy... - Ultrafast catalytic processes and conformational changes in the light-driven enzyme protochlorophyllide oxidoreductase (POR)Olga A Sytina
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, Amsterdam, The Netherlands
Biochem Soc Trans 37:387-91. 2009..This makes POR a very important model system to study the relationship between structural changes of enzymes and functionality...