M A Tryfonidou


Affiliation: Utrecht University
Country: The Netherlands


  1. Olthof M, Kempen D, Liu X, Dadsetan M, Tryfonidou M, Yaszemski M, et al. Effect of biomaterial electrical charge on in vivo bone formation. Tissue Eng Part A. 2019;: pubmed publisher
    ..Overall, this study clearly shows that negative charge enhances bone formation compared to neutral and positive charge in OPF composites. ..
  2. Rudnik Jansen I, Woike N, De Jong S, Versteeg S, Kik M, Emans P, et al. Applicability of a Modified Rat Model of Acute Arthritis for Long-Term Testing of Drug Delivery Systems. Pharmaceutics. 2019;11: pubmed publisher
  3. Olthof M, Lu L, Tryfonidou M, Loozen L, Pouran B, Yaszemski M, et al. The osteoinductive effect of controlled BMP-2 release is location-dependent. Tissue Eng Part A. 2018;: pubmed publisher
    ..Additionally, more bone formation in OPF-BP compared to ACS at both application sites emphasizes the role of biomaterials as a scaffold to achieve proper bone tissue formation. ..
  4. Tellegen A, Rudnik Jansen I, Beukers M, Miranda Bedate A, Bach F, De Jong W, et al. Intradiscal delivery of celecoxib-loaded microspheres restores intervertebral disc integrity in a preclinical canine model. J Control Release. 2018;286:439-450 pubmed publisher
    ..Follow-up studies are warranted to determine the clinical efficacy of celecoxib-loaded PEAMs in chronic back pain. ..
  5. Olthof M, Tryfonidou M, Dadsetan M, Dhert W, Yaszemski M, Kempen D, et al. In Vitro and In Vivo Correlation of BMP-2 Release Profiles from Complex Delivery Vehicles. Tissue Eng Part C Methods. 2018;: pubmed publisher
    ..Altogether, these results imply that more sophisticated in vitro systems should be used in bone regeneration to accurately discriminate and predict in vivo BMP-2 release from different complex delivery vehicles. ..
  6. de Vries S, Doeselaar M, Meij B, Tryfonidou M, Ito K. Notochordal cell matrix as a therapeutic agent for intervertebral disc regeneration. Tissue Eng Part A. 2018;: pubmed publisher
    ..Therefore NCM has potential in a regenerative therapy for disc degeneration, and warrants additional in vivo studies. ..
  7. Steen Louws C, Popov Celeketić J, Mastbergen S, Coeleveld K, Hack C, Eijkelkamp N, et al. IL4-10 fusion protein has chondroprotective, anti-inflammatory and potentially analgesic effects in the treatment of osteoarthritis. Osteoarthritis Cartilage. 2018;26:1127-1135 pubmed publisher
  8. Tellegen A, Willems N, Tryfonidou M, Meij B. Pedicle screw-rod fixation: a feasible treatment for dogs with severe degenerative lumbosacral stenosis. BMC Vet Res. 2015;11:299 pubmed publisher
    ..Pedicle screw-rod fixation alone does not result in interbody vertebral bone fusion between L7 and S1. ..
  9. Willems N, Tellegen A, Bergknut N, Creemers L, Wolfswinkel J, Freudigmann C, et al. Inflammatory profiles in canine intervertebral disc degeneration. BMC Vet Res. 2016;12:10 pubmed publisher
    ..Future studies are needed to investigate if inhibition of PGE2 levels in degenerated IVDs provides effective analgesia and exerts a protective role in the process of IVD degeneration and the development of IVD disease. ..

More Information


  1. Krouwels A, Melchels F, Van Rijen M, Oner F, Dhert W, Tryfonidou M, et al. Comparing Hydrogels for Human Nucleus Pulposus Regeneration: Role of Osmolarity During Expansion. Tissue Eng Part C Methods. 2018;24:222-232 pubmed publisher
    ..Although culture in SF medium reduces the amount of proteoglycans produced during redifferentiation culture, isolating and expanding the cells in high osmolarity medium can largely compensate for this loss. ..
  2. Willems N, Bach F, Plomp S, Van Rijen M, Wolfswinkel J, Grinwis G, et al. Intradiscal application of rhBMP-7 does not induce regeneration in a canine model of spontaneous intervertebral disc degeneration. Arthritis Res Ther. 2015;17:137 pubmed publisher
  3. Willems N, Yang H, Langelaan M, Tellegen A, Grinwis G, Kranenburg H, et al. Biocompatibility and intradiscal application of a thermoreversible celecoxib-loaded poly-N-isopropylacrylamide MgFe-layered double hydroxide hydrogel in a canine model. Arthritis Res Ther. 2015;17:214 pubmed publisher
  4. Bach F, Zhang Y, Miranda Bedate A, Verdonschot L, Bergknut N, Creemers L, et al. Increased caveolin-1 in intervertebral disc degeneration facilitates repair. Arthritis Res Ther. 2016;18:59 pubmed publisher
    ..Further studies are needed to investigate how caveolin-1 modifies other signaling pathways and facilitates IVD repair. ..
  5. Arkesteijn I, Smolders L, Spillekom S, Riemers F, Potier E, Meij B, et al. Effect of coculturing canine notochordal, nucleus pulposus and mesenchymal stromal cells for intervertebral disc regeneration. Arthritis Res Ther. 2015;17:60 pubmed publisher
    ..In this respect, NC-conditioned medium may be better than coculture for future studies of the regenerative potential of NCs. ..
  6. Olthof M, Tryfonidou M, Liu X, Pouran B, Meij B, Dhert W, et al. Phosphate functional groups improve OPF osteoconduction and BMP-2 osteoinductive efficacy. Tissue Eng Part A. 2017;: pubmed publisher
    ..Therefore, functionalizing hydrogels with phosphate groups by crosslinking monomers into the hydrogel matrix could provide a valuable method for improving polymer characteristics and hold great promise for bone tissue engineering. ..
  7. Smolders L, Meij B, Onis D, Riemers F, Bergknut N, Wubbolts R, et al. Gene expression profiling of early intervertebral disc degeneration reveals a down-regulation of canonical Wnt signaling and caveolin-1 expression: implications for development of regenerative strategies. Arthritis Res Ther. 2013;15:R23 pubmed publisher
    ..Therefore, Caveolin-1 may be regarded an exciting target for developing strategies for IVD regeneration. ..