Jan Tommassen

Summary

Affiliation: Utrecht University
Country: The Netherlands

Publications

  1. pmc An outer membrane receptor of Neisseria meningitidis involved in zinc acquisition with vaccine potential
    Michiel Stork
    Department of Molecular Microbiology, Utrecht University, Utrecht, The Netherlands
    PLoS Pathog 6:e1000969. 2010
  2. pmc Zinc piracy as a mechanism of Neisseria meningitidis for evasion of nutritional immunity
    Michiel Stork
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Utrecht, Netherlands
    PLoS Pathog 9:e1003733. 2013
  3. ncbi request reprint Assembly of outer-membrane proteins in bacteria and mitochondria
    Jan Tommassen
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Microbiology 156:2587-96. 2010
  4. ncbi request reprint Biochemistry. Getting into and through the outer membrane
    Jan Tommassen
    Department of Molecular Microbiology and the Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Science 317:903-4. 2007
  5. doi request reprint Supplementation of whole-cell pertussis vaccines with lipopolysaccharide analogs: modification of vaccine-induced immune responses
    Jeroen Geurtsen
    Department of Molecular Microbiology, Utrecht University, 3584 CH Utrecht, The Netherlands
    Vaccine 26:899-906. 2008
  6. ncbi request reprint Lipopolysaccharide transport to the bacterial outer membrane in spheroplasts
    Boris Tefsen
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Biol Chem 280:4504-9. 2005
  7. ncbi request reprint Immunogenicity and structural characterisation of an in vitro folded meningococcal siderophore receptor (FrpB, FetA)
    Jeroen Kortekaas
    Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Microbes Infect 8:2145-53. 2006
  8. ncbi request reprint MsbA is not required for phospholipid transport in Neisseria meningitidis
    Boris Tefsen
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Biol Chem 280:35961-6. 2005
  9. ncbi request reprint A novel secondary acyl chain in the lipopolysaccharide of Bordetella pertussis required for efficient infection of human macrophages
    Jeroen Geurtsen
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, Utrecht, The Netherlands
    J Biol Chem 282:37875-84. 2007
  10. pmc NalP-mediated proteolytic release of lactoferrin-binding protein B from the meningococcal cell surface
    Virginie Roussel-Jazédé
    Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Utrecht, Netherlands
    Infect Immun 78:3083-9. 2010

Collaborators

Detail Information

Publications75

  1. pmc An outer membrane receptor of Neisseria meningitidis involved in zinc acquisition with vaccine potential
    Michiel Stork
    Department of Molecular Microbiology, Utrecht University, Utrecht, The Netherlands
    PLoS Pathog 6:e1000969. 2010
    ..Furthermore, the results suggest that receptor-mediated zinc uptake represents a novel virulence mechanism that is particularly important for bacterial survival in the respiratory tract...
  2. pmc Zinc piracy as a mechanism of Neisseria meningitidis for evasion of nutritional immunity
    Michiel Stork
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Utrecht, Netherlands
    PLoS Pathog 9:e1003733. 2013
    ..Together, our results demonstrate that N. meningitidis is able to subvert an important defense mechanism of the human host and to utilize calprotectin to promote its growth...
  3. ncbi request reprint Assembly of outer-membrane proteins in bacteria and mitochondria
    Jan Tommassen
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Microbiology 156:2587-96. 2010
    ..These observations indicate that the basic mechanism of OMP assembly is evolutionarily highly conserved...
  4. ncbi request reprint Biochemistry. Getting into and through the outer membrane
    Jan Tommassen
    Department of Molecular Microbiology and the Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Science 317:903-4. 2007
  5. doi request reprint Supplementation of whole-cell pertussis vaccines with lipopolysaccharide analogs: modification of vaccine-induced immune responses
    Jeroen Geurtsen
    Department of Molecular Microbiology, Utrecht University, 3584 CH Utrecht, The Netherlands
    Vaccine 26:899-906. 2008
    ....
  6. ncbi request reprint Lipopolysaccharide transport to the bacterial outer membrane in spheroplasts
    Boris Tefsen
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Biol Chem 280:4504-9. 2005
    ..In contrast to LPS, de novo synthesized phospholipids were not transported to the outer membrane in spheroplasts. Apparently, LPS and phospholipids have different requirements for their transport to the outer membrane...
  7. ncbi request reprint Immunogenicity and structural characterisation of an in vitro folded meningococcal siderophore receptor (FrpB, FetA)
    Jeroen Kortekaas
    Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Microbes Infect 8:2145-53. 2006
    ..Our results show that in vitro folded FrpB assembles into oligomers, presumably dimers, and that it induces high levels of bactericidal antibodies in laboratory animals...
  8. ncbi request reprint MsbA is not required for phospholipid transport in Neisseria meningitidis
    Boris Tefsen
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Biol Chem 280:35961-6. 2005
    ..Although these results confirmed that MsbA functions in LPS transport, they also demonstrated that it is not required for phospholipid transport, at least not in N. meningitidis...
  9. ncbi request reprint A novel secondary acyl chain in the lipopolysaccharide of Bordetella pertussis required for efficient infection of human macrophages
    Jeroen Geurtsen
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, Utrecht, The Netherlands
    J Biol Chem 282:37875-84. 2007
    ..In addition, we show that an lpxL1-deficient mutant of B. pertussis displays a defect in the infection of human macrophages...
  10. pmc NalP-mediated proteolytic release of lactoferrin-binding protein B from the meningococcal cell surface
    Virginie Roussel-Jazédé
    Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Utrecht, Netherlands
    Infect Immun 78:3083-9. 2010
    ..Since antibodies directed against LbpB are found in convalescent-patient sera, the release of an immunogenic protein as LbpB may represent a novel means for N. meningitidis to escape the human immune response...
  11. pmc Crystal structure and catalytic mechanism of the LPS 3-O-deacylase PagL from Pseudomonas aeruginosa
    Lucy Rutten
    Department of Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 103:7071-6. 2006
    ..In addition, an aspartate makes a hydrogen bond with the hydroxyl group of the 3-O-acyl chain, probably providing specificity of PagL toward lipid A...
  12. pmc Species-specificity of the BamA component of the bacterial outer membrane protein-assembly machinery
    Elena B Volokhina
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    PLoS ONE 8:e85799. 2013
    ..Furthermore, conformational analysis of chimeric proteins provided evidence for a 16-stranded β-barrel conformation of the membrane-embedded domain of BamA. ..
  13. doi request reprint Channel properties of the translocator domain of the autotransporter Hbp of Escherichia coli
    Virginie Roussel-Jazédé
    Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    Mol Membr Biol 28:158-70. 2011
    ..In addition, we could show that the α-helix contributes to the stability of TDs...
  14. ncbi request reprint Shielding of immunogenic domains in Neisseria meningitidis FrpB (FetA) by the major variable region
    Jeroen Kortekaas
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan, 3584 CH Utrecht, The Netherlands
    Vaccine 25:72-84. 2007
    ....
  15. pmc Coincorporation of LpxL1 and PagL mutant lipopolysaccharides into liposomes with Neisseria meningitidis opacity protein: influence on endotoxic and adjuvant activity
    Jesús Arenas
    Department of Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Clin Vaccine Immunol 17:487-95. 2010
    ..In conclusion, PagL LPS is a better adjuvant than LpxL1 LPS, but coincorporation of either LPS into proteoliposomes did not improve their adjuvant activity...
  16. pmc Structure of the translocator domain of a bacterial autotransporter
    Clasien J Oomen
    Department of Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands
    EMBO J 23:1257-66. 2004
    ....
  17. doi request reprint Polar secretion of proteins via the Xcp type II secretion system in Pseudomonas aeruginosa
    Freya Senf
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Microbiology 154:3025-32. 2008
    ..The polar location of the Xcp machinery was further confirmed by the visualization of protease secretion with an intramolecularly quenched casein conjugate...
  18. pmc Assembly factor Omp85 recognizes its outer membrane protein substrates by a species-specific C-terminal motif
    Viviane Robert
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    PLoS Biol 4:e377. 2006
    ..coli outer membrane was accomplished after adapting its signature sequence. These results demonstrate that the Omp85 assembly machinery recognizes OMPs by virtue of their C-terminal signature sequence...
  19. pmc Cellular solid-state nuclear magnetic resonance spectroscopy
    Marie Renault
    Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 109:4863-8. 2012
    ..Our approach permits studying entire cellular compartments as well as cell-associated proteins at the same time and at atomic resolution...
  20. pmc The beta-barrel outer membrane protein assembly complex of Neisseria meningitidis
    Elena B Volokhina
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Bacteriol 191:7074-85. 2009
    ..Thus, the Bam complex in N. meningitidis consists of Omp85/BamA plus RmpM, BamC, ComL/BamD, and BamE, of which ComL/BamD and BamE appear to be the most important accessory components for OMP assembly...
  21. ncbi request reprint Consequences of the expression of lipopolysaccharide-modifying enzymes for the efficacy and reactogenicity of whole-cell pertussis vaccines
    Jeroen Geurtsen
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3485 CH Utrecht, The Netherlands
    Microbes Infect 9:1096-103. 2007
    ..These data show, for the first time, that lipopolysaccharide composition is an important determinant for the efficacy of whole-cell pertussis vaccines...
  22. ncbi request reprint Defective translocation of a signal sequence mutant in a prlA4 suppressor strain of Escherichia coli
    Hendrik Adams
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, The Netherlands
    Eur J Biochem 269:5572-80. 2002
    ..Together, these data suggest that SecB and SRP substrates are differently processed at the SecYEG translocon...
  23. pmc Interactions between phage-shock proteins in Escherichia coli
    Hendrik Adams
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Bacteriol 185:1174-80. 2003
    ..Furthermore, affinity-chromatography suggested that PspB could bind PspA only in the presence of PspC. These data indicate that regulation of the psp operon is mediated via protein-protein interactions...
  24. pmc Identification of a novel lipopolysaccharide core biosynthesis gene cluster in Bordetella pertussis, and influence of core structure and lipid A glucosamine substitution on endotoxic activity
    Jeroen Geurtsen
    Department of Molecular Microbiology, Utrecht University, 3584 CH Utrecht, The Netherlands
    Infect Immun 77:2602-11. 2009
    ....
  25. pmc Role of the pilot protein YscW in the biogenesis of the YscC secretin in Yersinia enterocolitica
    Peter Burghout
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Bacteriol 186:5366-75. 2004
    ..Hence, YscW interacts with the unassembled YscC protein and facilitates efficient oligomerization, likely at the outer membrane...
  26. ncbi request reprint Interaction domains in the Pseudomonas aeruginosa type II secretory apparatus component XcpS (GspF)
    Jorik Arts
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Microbiology 153:1582-92. 2007
    ..The cytoplasmic loop of XcpS was found to be involved in the stabilization by XcpR and XcpY...
  27. ncbi request reprint A novel phase-variable autotransporter serine protease, AusI, of Neisseria meningitidis
    Peter van Ulsen
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Microbes Infect 8:2088-97. 2006
    ..In silico sequence analysis suggested several putative functions for AusI, which, however, could not be confirmed experimentally...
  28. ncbi request reprint Dissemination of lipid A deacylases (pagL) among gram-negative bacteria: identification of active-site histidine and serine residues
    Jeroen Geurtsen
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, Utrecht 3584 CH, The Netherlands
    J Biol Chem 280:8248-59. 2005
    ..The biological function of PagL remains unclear. However, because PagL homologs were found in both pathogenic and nonpathogenic species, PagL-mediated deacylation of lipid A probably does not have a dedicated role in pathogenicity...
  29. pmc Two-partner secretion systems of Neisseria meningitidis associated with invasive clonal complexes
    Peter van Ulsen
    Department of Molecular Microbiology, Utrecht University, 3584 CH Utrecht, The Netherlands
    Infect Immun 76:4649-58. 2008
    ..Together, our data indicate that TPS systems may contribute to the virulence of N. meningitidis...
  30. pmc Role of the periplasmic chaperones Skp, SurA, and DegQ in outer membrane protein biogenesis in Neisseria meningitidis
    Elena B Volokhina
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, Netherlands
    J Bacteriol 193:1612-21. 2011
    ..meningitidis was heterologously expressed in skp mutants, indicating that Skp functions in a species-specific manner. Our results demonstrate an important role for Skp but not for SurA or DegQ in OMP biogenesis in N. meningitidis...
  31. ncbi request reprint Vaccine potential of the Neisseria meningitidis lactoferrin-binding proteins LbpA and LbpB
    Annika Pettersson
    Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Vaccine 24:3545-57. 2006
    ..Our results show that both proteins are immunogenic and able to induce bactericidal antibodies, but that the cross-reactivity of these antibodies is limited...
  32. ncbi request reprint Role of a highly conserved bacterial protein in outer membrane protein assembly
    Rome Voulhoux
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, Netherlands
    Science 299:262-5. 2003
    ..Immunofluorescence microscopy revealed decreased surface exposure of outer membrane proteins, which was particularly apparent at the cell-division planes. Thus, Omp85 is likely to play a role in outer membrane protein assembly...
  33. pmc Species-specific functioning of the Pseudomonas XcpQ secretin: role for the C-terminal homology domain and lipopolysaccharide
    Wilbert Bitter
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Bacteriol 189:2967-75. 2007
    ..alcaligenes XcpQ and inactive hybrids to function properly in P. aeruginosa. Since divalent cations are known to affect the structure of LPS, this observation supports the hypothesis that LPS has a role in the functioning of secretins...
  34. doi request reprint The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatases
    Florian Putker
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH, Utrecht, The Netherlands
    Environ Microbiol 15:2658-71. 2013
    ..Both Tat systems appeared to be capable of transporting the UxpB protein. However, expression of the tat-1 genes was strongly induced by low Pi levels, indicating a function of this system in survival during Pi starvation. ..
  35. pmc Structure and electrophysiological properties of the YscC secretin from the type III secretion system of Yersinia enterocolitica
    Peter Burghout
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    J Bacteriol 186:4645-54. 2004
    ..This structure is consistent with the notion that the secretin forms the upper part of the basal body of the needle structure of the type III secreton...
  36. pmc Export of the pseudopilin XcpT of the Pseudomonas aeruginosa type II secretion system via the signal recognition particle-Sec pathway
    Jorik Arts
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Bacteriol 189:2069-76. 2007
    ..Experiments with conditional sec mutants and reporter-protein fusions showed that this transport process involves the cotranslational signal recognition particle targeting route and is dependent on a functional Sec translocon...
  37. pmc Functioning of outer membrane protein assembly factor Omp85 requires a single POTRA domain
    Martine P Bos
    Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    EMBO Rep 8:1149-54. 2007
    ..Thus, similar to the mitochondrial Omp85 homologue, the functional core of bacterial Omp85 consists of its membrane domain and a single POTRA domain, that is, POTRA5...
  38. pmc Identification of an outer membrane protein required for the transport of lipopolysaccharide to the bacterial cell surface
    Martine P Bos
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH, Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 101:9417-22. 2004
    ..The role of Imp in LPS biogenesis and its high conservation among Gram-negative bacteria make it an excellent target for the development of novel antibacterial compounds...
  39. ncbi request reprint Biogenesis of the gram-negative bacterial outer membrane
    Martine P Bos
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Annu Rev Microbiol 61:191-214. 2007
    ..Recent studies in Neisseria meningitidis and Escherichia coli have led to the identification of several machineries implicated in these transport and assembly processes...
  40. ncbi request reprint Solid-state NMR studies of full-length BamA in lipid bilayers suggest limited overall POTRA mobility
    Tessa Sinnige
    NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Mol Biol 426:2009-21. 2014
    ..The observed interplay between protein-protein and protein-lipid interactions may be critical for BamA-mediated insertion of substrates into the outer membrane. ..
  41. doi request reprint Autotransporter secretion: varying on a theme
    Jan Grijpstra
    Section Molecular Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Res Microbiol 164:562-82. 2013
    ..We review here the current knowledge of the biogenesis, structure and function of various autotransporters. ..
  42. pmc Ght protein of Neisseria meningitidis is involved in the regulation of lipopolysaccharide biosynthesis
    Florian Putker
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Utrecht, Netherlands
    J Bacteriol 196:780-9. 2014
    ..We suggest that Ght regulates LPS biosynthesis by affecting the activity of LpxC. Possibly, this mechanism acts in the previously observed feedback inhibition of LPS synthesis that occurs when LPS transport is hampered. ..
  43. doi request reprint Lipidation of the autotransporter NalP of Neisseria meningitidis is required for its function in the release of cell-surface-exposed proteins
    Virginie Roussel-Jazédé
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    Microbiology 159:286-95. 2013
    ..Thus, the lipid moiety delays the release of NalP from the cell surface, thereby allowing it to release other surface-exposed proteins into the milieu...
  44. pmc The LptD chaperone LptE is not directly involved in lipopolysaccharide transport in Neisseria meningitidis
    Martine P Bos
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Biol Chem 286:28688-96. 2011
    ..Apparently, the role of LptE in N. meningitidis deviates from that in E. coli, suggesting that the Lpt system does not function in a completely conserved manner in all gram-negative bacteria...
  45. pmc Function of neisserial outer membrane phospholipase a in autolysis and assessment of its vaccine potential
    Martine P Bos
    Department of Molecular Microbiology and Institute for Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Infect Immun 73:2222-31. 2005
    ..Also, convalescent patients and vaccinee sera did not contain detectable levels of anti-OMPLA antibodies, indicating that OMPLA may not be sufficiently immunogenic to be included in a meningococcal vaccine...
  46. doi request reprint Involvement of three meningococcal surface-exposed proteins, the heparin-binding protein NhbA, the α-peptide of IgA protease and the autotransporter protease NalP, in initiation of biofilm formation
    Jesús Arenas
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Mol Microbiol 87:254-68. 2013
    ..We conclude that NhbA and the α-peptide of IgA protease are implicated in biofilm formation by binding eDNA and that NalP is an important regulator of this process through the proteolysis of these surface-exposed proteins...
  47. doi request reprint Solid-state NMR on a large multidomain integral membrane protein: the outer membrane protein assembly factor BamA
    Marie Renault
    Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Am Chem Soc 133:4175-7. 2011
    ..This approach hence provides a general strategy for collecting atomic-scale structural information on multidomain (membrane) proteins in a native-like environment...
  48. ncbi request reprint The presence of a helix breaker in the hydrophobic core of signal sequences of secretory proteins prevents recognition by the signal-recognition particle in Escherichia coli
    Hendrik Adams
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, The Netherlands
    Eur J Biochem 269:5564-71. 2002
    ..Together, these data indicate that the helix breaker in cleavable signal sequences prevents recognition by SRP and is thereby, together with the hydrophobicity of the signal sequence, a determinant of the targeting pathway...
  49. pmc Llama single-chain antibody that blocks lipopolysaccharide binding and signaling: prospects for therapeutic applications
    Mohamed El Khattabi
    Department of Cellular Architecture and Dynamics, Faculty of Science, Utrecht University, Utrecht, The Netherlands
    Clin Vaccine Immunol 13:1079-86. 2006
    ..Moreover, it was found to remove LPS efficiently from aqueous solutions, including serum. The selected anti-LPS VHH is a leading candidate for therapies against LPS-mediated sepsis...
  50. pmc Lipopolysaccharide analogs improve efficacy of acellular pertussis vaccine and reduce type I hypersensitivity in mice
    Jeroen Geurtsen
    Department of Molecular Microbiology, Utrecht University, 3584 CH Utrecht, The Netherlands
    Clin Vaccine Immunol 14:821-9. 2007
    ..In conclusion, these results indicate that supplementation with LPS analogs forms a promising strategy that can be used to improve aP vaccines...
  51. ncbi request reprint Omp85, an evolutionarily conserved bacterial protein involved in outer-membrane-protein assembly
    Rome Voulhoux
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Res Microbiol 155:129-35. 2004
    ..Omp85 shows sequence similarity with Toc75 of the chloroplast protein-import machinery, suggesting a common evolutionary origin...
  52. doi request reprint Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach
    Peter van Ulsen
    Department of Molecular Microbiology, Utrecht University, Utrecht, The Netherlands
    Proteomics 9:1771-81. 2009
    ..These 11 proteins include proteins likely involved in iron metabolism...
  53. ncbi request reprint Protein secretion and secreted proteins in pathogenic Neisseriaceae
    Peter van Ulsen
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    FEMS Microbiol Rev 30:292-319. 2006
    ..We hypothesize that these ORFs may substitute for the corresponding fragments in the full-length genes through genetic recombination, thereby changing the host-cell receptor specificity of the secreted protein...
  54. ncbi request reprint Biogenesis of the Gram-negative bacterial outer membrane
    Martine P Bos
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Curr Opin Microbiol 7:610-6. 2004
    ....
  55. pmc Viability of a capsule- and lipopolysaccharide-deficient mutant of Neisseria meningitidis
    Martine P Bos
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Infect Immun 73:6194-7. 2005
    ..It was thought that the presence of capsular polysaccharide is necessary for this unusual feature. However, we show now that no part of the capsule gene cluster is required for maintaining LPS deficiency in N. meningitidis...
  56. pmc Pseudomonas evades immune recognition of flagellin in both mammals and plants
    Bart W Bardoel
    Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS Pathog 7:e1002206. 2011
    ..Thus, by degrading the ligand for TLR5 and FLS2, P. aeruginosa escapes recognition by the innate immune systems of both mammals and plants...
  57. ncbi request reprint Crystal structure of Neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential
    Lucy Vandeputte-Rutten
    Department of Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Utrecht, The Netherlands
    J Biol Chem 278:24825-30. 2003
    ..In addition, the structure provides a starting point for structure-based vaccine design...
  58. ncbi request reprint A Neisserial autotransporter NalP modulating the processing of other autotransporters
    Peter van Ulsen
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    Mol Microbiol 50:1017-30. 2003
    ..We propose that, apart from the autoproteolytic processing, NalP can process App and IgA protease and hypothesize that this function of NalP could contribute to the virulence of the organism...
  59. ncbi request reprint Involvement of Neisseria meningitidis Lipoprotein GNA2091 in the Assembly of a Subset of Outer Membrane Proteins
    Martine P Bos
    From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Biol Chem 289:15602-10. 2014
    ..Furthermore, its location at the inner side of the outer membrane indicates that protective immunity elicited by this antigen cannot be due to bactericidal or opsonic activity of antibodies. ..
  60. pmc Domain exchange at the 3' end of the gene encoding the fratricide meningococcal two-partner secretion protein A
    Jesús Arenas
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, Utrecht 3584 CH, The Netherlands
    BMC Genomics 14:622. 2013
    ..Here, we investigated (i) whether TpsA of N. meningitidis also has growth-inhibitory properties, (ii) whether tpsC cassettes recombine with the tpsA gene, and (iii) what the consequences of such recombination events might be...
  61. doi request reprint Assembly of bacterial outer membrane proteins
    Jan Grijpstra
    Department of Molecular Microbiology, Utrecht University, Utrecht, The Netherlands
    Methods Mol Biol 966:223-37. 2013
    ....
  62. ncbi request reprint Solid-state NMR spectroscopy on cellular preparations enhanced by dynamic nuclear polarization
    Marie Renault
    Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht The Netherlands
    Angew Chem Int Ed Engl 51:2998-3001. 2012
    ..The major molecular components in the cells can be characterized in this way...
  63. ncbi request reprint PspE (phage-shock protein E) of Escherichia coli is a rhodanese
    Hendrik Adams
    Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    FEBS Lett 518:173-6. 2002
    ..8.1.1; rhodanese). Kinetic analysis revealed that catalysis occurs via a double displacement mechanism as described for other rhodaneses. The K(m)s for SSO3(2-) and CN- were 4.6 and 27 mM, respectively...
  64. pmc Hexadecane and Tween 80 stimulate lipase production in Burkholderia glumae by different mechanisms
    Bouke K H L Boekema
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Appl Environ Microbiol 73:3838-44. 2007
    ..In conclusion, hexadecane and Tween 80 enhance lipase production in B. glumae, and they act via different mechanisms...
  65. pmc Role of Pseudomonas putida tol-oprL gene products in uptake of solutes through the cytoplasmic membrane
    María A Llamas
    Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, 18008 Granada, Spain
    J Bacteriol 185:4707-16. 2003
    ..These results suggest that the Tol-OprL system is necessary for appropriate functioning of certain uptake systems at the level of the cytoplasmic membrane...
  66. ncbi request reprint Conformational analysis of opacity proteins from Neisseria meningitidis
    Marien I de Jonge
    Laboratory of Vaccine Research, National Institute of Public Health and the Environment, RIVM Bilthoven, The Netherlands
    Eur J Biochem 269:5215-23. 2002
    ..denatured OpaJ protein to the N-A1 domain of CEACAM1. Almost no binding was found with the denatured Opa protein, showing that the Opa-receptor interaction is conformation-dependent...
  67. ncbi request reprint Three homologues, including two membrane-bound proteins, of the disulfide oxidoreductase DsbA in Neisseria meningitidis: effects on bacterial growth and biogenesis of functional type IV pili
    Colin R Tinsley
    INSERM U570, Faculte de Medecine Necker, 156 rue de Vaugirard, 75730 Paris Cedex 15, France
    J Biol Chem 279:27078-87. 2004
    ....
  68. pmc Polar localization of the autotransporter family of large bacterial virulence proteins
    Sumita Jain
    Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, 65 Landsdowne St, Cambridge, MA 02139, USA
    J Bacteriol 188:4841-50. 2006
    ....
  69. pmc Expression of the lipopolysaccharide-modifying enzymes PagP and PagL modulates the endotoxic activity of Bordetella pertussis
    Jeroen Geurtsen
    Netherlands Vaccine Institute, P O Box 457, 3720 AL Bilthoven, The Netherlands
    Infect Immun 74:5574-85. 2006
    ..However, more unexpectedly, this was also the case for PagL. This paradoxical result may be explained, in part, by an increased release of LPS, which we observed in the PagL-expressing cells...
  70. ncbi request reprint Lipase-specific foldases
    Frank Rosenau
    Institut für Molekulare Enzymtechnologie, Heinrich Heine Universitat Dusseldorf, Forschungszentrum Julich, 52428 Jülich, Germany
    Chembiochem 5:152-61. 2004
    ....
  71. ncbi request reprint Teasing apart structural determinants of 'toxicity' and 'adjuvanticity': implications for meningococcal vaccine development
    Liana Steeghs
    Laboratory of Immunotherapy, Department of Immunology, University Medical Centre Utrecht, The Netherlands
    J Endotoxin Res 10:113-9. 2004
    ..The availability of this novel set of lipid A variants with improved pharmacological properties will be of great importance for the improvement of future outer membrane vesicle vaccines against N. meningitidis...
  72. pmc Identification and characterization of a novel outer membrane protein (OMP J) of Moraxella catarrhalis that exists in two major forms
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Bacteriol 187:7977-84. 2005
    ....
  73. ncbi request reprint Identification of a novel Gsp-related pathway required for secretion of the manganese-oxidizing factor of Pseudomonas putida strain GB-1
    Johannes De Vrind
    Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, 2300 RA Leiden, The Netherlands
    Mol Microbiol 47:993-1006. 2003
    ..They do contain xcpR and xcpQ homologues but, for example, homologues of xcpP, Y and Z are lacking. The results suggest a novel Xcp-related system for the transport of manganese-oxidizing enzymes to the cell surface...
  74. ncbi request reprint Structure of a membrane-based steric chaperone in complex with its lipase substrate
    Kris Pauwels
    Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute for Biotechnology VIB and Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium
    Nat Struct Mol Biol 13:374-5. 2006
    ..The structure reveals how Lif uses a novel alpha-helical scaffold to embrace lipase, thereby creating an unusually extensive folding platform...
  75. ncbi request reprint Chaperoning Anfinsen: the steric foldases
    Kris Pauwels
    Department of Molecular and Cellular Interactions, VIB and Department of Ultrastructure, Free University Brussels, Brussels, Belgium
    Mol Microbiol 64:917-22. 2007
    ..The case of the pilin chaperone is somewhat deviant in that steric information is definitely provided, but the pilus subunit adopts a thermodynamically favourable stable conformation...