Peter J M Rottier

Summary

Affiliation: Utrecht University
Country: The Netherlands

Publications

  1. ncbi Spike protein assembly into the coronavirion: exploring the limits of its sequence requirements
    Berend Jan Bosch
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    Virology 334:306-18. 2005
  2. pmc Acquisition of macrophage tropism during the pathogenesis of feline infectious peritonitis is determined by mutations in the feline coronavirus spike protein
    Peter J M Rottier
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 79:14122-30. 2005
  3. pmc Murine coronavirus with an extended host range uses heparan sulfate as an entry receptor
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Yalelaan 1, 3584CL Utrecht, The Netherlands
    J Virol 79:14451-6. 2005
  4. pmc Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Yalelaan 1, 3584CL Utrecht, The Netherlands
    J Virol 78:6048-54. 2004
  5. pmc The ubiquitin-proteasome system plays an important role during various stages of the coronavirus infection cycle
    Matthijs Raaben
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands
    J Virol 84:7869-79. 2010
  6. pmc Dynamics of coronavirus replication-transcription complexes
    Marne C Hagemeijer
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 84:2134-49. 2010
  7. pmc Live, attenuated coronavirus vaccines through the directed deletion of group-specific genes provide protection against feline infectious peritonitis
    Bert Jan Haijema
    Institute of Virology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
    J Virol 78:3863-71. 2004
  8. pmc Spike protein fusion peptide and feline coronavirus virulence
    Hui Wen Chang
    Virology Division, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University, Utrecht, The Netherlands
    Emerg Infect Dis 18:1089-95. 2012
  9. doi Identification of host factors involved in coronavirus replication by quantitative proteomics analysis
    Mijke W Vogels
    Department of Biochemistry and Cell Biology, Biochemistry Division, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    Proteomics 11:64-80. 2011
  10. doi Feline infectious peritonitis: insights into feline coronavirus pathobiogenesis and epidemiology based on genetic analysis of the viral 3c gene
    Hui Wen Chang
    Virology Division, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University, Utrecht, The Netherlands
    J Gen Virol 91:415-20. 2010

Collaborators

Detail Information

Publications72

  1. ncbi Spike protein assembly into the coronavirion: exploring the limits of its sequence requirements
    Berend Jan Bosch
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    Virology 334:306-18. 2005
    ..The important role of the about 38-residues cytoplasmic domain in the assembly of and membrane fusion by this approximately 1300 amino acids long protein is discussed...
  2. pmc Acquisition of macrophage tropism during the pathogenesis of feline infectious peritonitis is determined by mutations in the feline coronavirus spike protein
    Peter J M Rottier
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 79:14122-30. 2005
    ..The implications of these findings are discussed...
  3. pmc Murine coronavirus with an extended host range uses heparan sulfate as an entry receptor
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Yalelaan 1, 3584CL Utrecht, The Netherlands
    J Virol 79:14451-6. 2005
    ....
  4. pmc Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Yalelaan 1, 3584CL Utrecht, The Netherlands
    J Virol 78:6048-54. 2004
    ..While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements...
  5. pmc The ubiquitin-proteasome system plays an important role during various stages of the coronavirus infection cycle
    Matthijs Raaben
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands
    J Virol 84:7869-79. 2010
    ..Our observations reveal an important role of the UPS in multiple steps of the CoV infection cycle and identify the UPS as a potential drug target to modulate the impact of CoV infection...
  6. pmc Dynamics of coronavirus replication-transcription complexes
    Marne C Hagemeijer
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 84:2134-49. 2010
    ..The observations point to intriguing and as yet unrecognized similarities between the RTC dynamics of different plus-strand RNA viruses...
  7. pmc Live, attenuated coronavirus vaccines through the directed deletion of group-specific genes provide protection against feline infectious peritonitis
    Bert Jan Haijema
    Institute of Virology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
    J Virol 78:3863-71. 2004
    ..More generally, our approach may allow the development of vaccines against infections with other pathogenic coronaviruses, including that causing severe acute respiratory syndrome in humans...
  8. pmc Spike protein fusion peptide and feline coronavirus virulence
    Hui Wen Chang
    Virology Division, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University, Utrecht, The Netherlands
    Emerg Infect Dis 18:1089-95. 2012
    ..By these and perhaps other mutations, the virus apparently acquires its macrophage tropism and spreads systemically...
  9. doi Identification of host factors involved in coronavirus replication by quantitative proteomics analysis
    Mijke W Vogels
    Department of Biochemistry and Cell Biology, Biochemistry Division, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    Proteomics 11:64-80. 2011
    ..Overall, our study shows that the SILAC approach is a suitable tool to study host-pathogen interactions and to identify host proteins involved in virus replication...
  10. doi Feline infectious peritonitis: insights into feline coronavirus pathobiogenesis and epidemiology based on genetic analysis of the viral 3c gene
    Hui Wen Chang
    Virology Division, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University, Utrecht, The Netherlands
    J Gen Virol 91:415-20. 2010
    ..Apparently, 3c-inactivated viruses replicate not at all--or only poorly--in the gut, explaining the rare incidence of FIP outbreaks...
  11. pmc Cooperative involvement of the S1 and S2 subunits of the murine coronavirus spike protein in receptor binding and extended host range
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 80:10909-18. 2006
    ..e., regions far apart in the linear sequence, communicate and may even interact physically in the higher-order structure of the spike...
  12. pmc A protective and safe intranasal RSV vaccine based on a recombinant prefusion-like form of the F protein bound to bacterium-like particles
    Alan Rigter
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    PLoS ONE 8:e71072. 2013
    ....
  13. pmc Influenza A virus entry into cells lacking sialylated N-glycans
    Erik de Vries
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 109:7457-62. 2012
    ..Our results indicate that the balance between HA and NA is important not only for virion release, but also for entry into cells...
  14. pmc The proteasome inhibitor Velcade enhances rather than reduces disease in mouse hepatitis coronavirus-infected mice
    Matthijs Raaben
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands
    J Virol 84:7880-5. 2010
    ..Surprisingly, however, the drug did not reduce replication of the virus in mice. Rather, inhibition of the proteasome caused enhanced infection with lethal outcome, calling for caution when using this type of drug during infection...
  15. pmc Structural protein requirements in equine arteritis virus assembly
    Roeland Wieringa
    Virology Division, Department of Infectious Diseases and Immunology, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 78:13019-27. 2004
    ....
  16. pmc Cathepsin L functionally cleaves the severe acute respiratory syndrome coronavirus class I fusion protein upstream of rather than adjacent to the fusion peptide
    Berend Jan Bosch
    Department of Infectious Diseases and Immunology, Virology Division, Faculty of Veterinary Medicine, Utrecht University, Institute of Biomembranes, Utrecht, The Netherlands
    J Virol 82:8887-90. 2008
    ..Moreover, cleavage was mapped to the same region where, in coronaviruses carrying furin-activated spikes, the receptor binding subunit of the protein is separated from the membrane-anchored fusion subunit...
  17. pmc Type I interferon receptor-independent and -dependent host transcriptional responses to mouse hepatitis coronavirus infection in vivo
    Matthijs Raaben
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    BMC Genomics 10:350. 2009
    ..The protective effectors against MHV infection are, however, still unknown...
  18. pmc The coronavirus nucleocapsid protein is dynamically associated with the replication-transcription complexes
    Monique H Verheije
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Yalelaan 1, 3584 CL Utrecht, Netherlands
    J Virol 84:11575-9. 2010
    ..Recruitment of the N protein to the RTCs requires the C-terminal N2b domain, which interacts with other N proteins in an RNA-independent manner...
  19. ncbi Manipulation of the coronavirus genome using targeted RNA recombination with interspecies chimeric coronaviruses
    Cornelis A M de Haan
    Virology Division, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
    Methods Mol Biol 454:229-36. 2008
    ..g., murine or feline) cell tropism. Additional mutations created in the donor RNA can be co-incorporated into the recombinant virus in order to generate mutant viruses...
  20. pmc An autophagy-independent role for LC3 in equine arteritis virus replication
    Iryna Monastyrska
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
    Autophagy 9:164-74. 2013
    ....
  21. pmc Coronavirus spike glycoprotein, extended at the carboxy terminus with green fluorescent protein, is assembly competent
    Berend Jan Bosch
    Department of Infectious Diseases and Immunology, Yalelaan 1, 3584CL Utrecht, The Netherlands
    J Virol 78:7369-78. 2004
    ..The fluorescent MHV-SGFP will now be a convenient tool to study coronaviral cell entry...
  22. pmc Topology and membrane anchoring of the coronavirus replication complex: not all hydrophobic domains of nsp3 and nsp6 are membrane spanning
    Monique Oostra
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 82:12392-405. 2008
    ..The occurrence of conserved non-membrane-spanning hydrophobic domains in nsp3 and nsp6 suggests an important function for these domains in coronavirus replication...
  23. pmc The 29-nucleotide deletion present in human but not in animal severe acute respiratory syndrome coronaviruses disrupts the functional expression of open reading frame 8
    Monique Oostra
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Virol 81:13876-88. 2007
    ..Clearly, the 29-nucleotide deletion disrupts the proper expression of the SARS-CoV ORF8, the implications of which are discussed...
  24. pmc Coronaviruses as vectors: position dependence of foreign gene expression
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine and Institute of Biomembranes, Utrecht University, 3584 CL Utrecht, The Netherlands
    J Virol 77:11312-23. 2003
    ..Altogether, our observations clearly demonstrate the potential of coronaviruses as (multivalent) expression vectors...
  25. pmc Intra- and intermolecular disulfide bonds of the GP2b glycoprotein of equine arteritis virus: relevance for virus assembly and infectivity
    Roeland Wieringa
    Department of Infectious Diseases and Immunology, Virology Division, Faculty of Veterinary Medicine, and Institute of Biomembranes, Utrecht University, 3584 CL Utrecht, The Netherlands
    J Virol 77:12996-3004. 2003
    ..In addition, our data highlight the importance of the correct association of the minor EAV envelope glycoproteins for their efficient incorporation into viral particles and for virus infectivity...
  26. pmc Switching species tropism: an effective way to manipulate the feline coronavirus genome
    Bert Jan Haijema
    Institute of Virology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
    J Virol 77:4528-38. 2003
    ..We establish the second targeted RNA recombination system for coronaviruses and provide a powerful tool for the genetic engineering of the FIPV genome...
  27. pmc Competition between influenza A virus genome segments
    Ivy Widjaja
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    PLoS ONE 7:e47529. 2012
    ..This competition is probably most apparent early during infection, when limiting amounts of polymerases are present, and may contribute to the regulation of segment-specific replication and transcription...
  28. doi Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle
    Mijke W Vogels
    Department of Biochemistry and Cell Biology, Biochemistry Division, Utrecht University, Utrecht, The Netherlands
    Proteomics 11:4477-91. 2011
    ..It can be concluded that by using SILAC-based quantitative proteomics we were able to identify novel host cell proteins involved in the complex interplay between Salmonella and epithelial cells...
  29. ncbi The influenza A virus hemagglutinin glycosylation state affects receptor-binding specificity
    Robert P de Vries
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    Virology 403:17-25. 2010
    ..As a consequence, differences in HA fine receptor specificities could not be observed with the insect cell-, but were readily detected with the HEK293S GnTI(-) cell-produced HAs...
  30. ncbi Hosting the severe acute respiratory syndrome coronavirus: specific cell factors required for infection
    Cornelis A M de Haan
    Division Virology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    Cell Microbiol 8:1211-8. 2006
    ..In this brief review, we describe the current understanding of how the SARS-CoV is able to use the cellular machinery for its replication...
  31. doi Non-invasive imaging of mouse hepatitis coronavirus infection reveals determinants of viral replication and spread in vivo
    Matthijs Raaben
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    Cell Microbiol 11:825-41. 2009
    ..Our results provide new insights in coronavirus pathogenesis and demonstrate the potential of BLI to study coronavirus-host interactions in vivo...
  32. pmc Pathogenic characteristics of persistent feline enteric coronavirus infection in cats
    Liesbeth Vogel
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    Vet Res 41:71. 2010
    ..The implications of our results, particularly our insights into the persistently infected state, are discussed...
  33. pmc Mobility and interactions of coronavirus nonstructural protein 4
    Marne C Hagemeijer
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, Netherlands
    J Virol 85:4572-7. 2011
    ..In addition, the coexpression of nsp4 with nsp3 affected the subcellular localization of the two proteins...
  34. pmc Severe acute respiratory syndrome coronavirus (SARS-CoV) infection inhibition using spike protein heptad repeat-derived peptides
    Berend Jan Bosch
    Division of Virology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, and Institute of Biomembranes, Utrecht University, 3584 CL Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 101:8455-60. 2004
    ..The inhibitory potency of the SARS-CoV HR2-peptides provides an attractive basis for the development of a therapeutic drug for SARS...
  35. pmc Gain, preservation, and loss of a group 1a coronavirus accessory glycoprotein
    Alessio Lorusso
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 82:10312-7. 2008
    ..Our findings provide insight into the evolutionary history of coronavirus group 1a and into the dynamics of gain and loss of accessory genes...
  36. pmc The receptor binding domain of the new Middle East respiratory syndrome coronavirus maps to a 231-residue region in the spike protein that efficiently elicits neutralizing antibodies
    Huihui Mou
    Department of Infectious Diseases and Immunology, Virology Division, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Virol 87:9379-83. 2013
    ..Antibodies to this domain--much less so those to the preceding N-terminal region--efficiently neutralize MERS-CoV infection...
  37. pmc Dissection of the influenza A virus endocytic routes reveals macropinocytosis as an alternative entry pathway
    Erik de Vries
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    PLoS Pathog 7:e1001329. 2011
    ..Thus, in addition to the classical dynamin-dependent, clathrin-mediated endocytosis pathway, IAV enters host cells by a dynamin-independent route that has all the characteristics of macropinocytosis...
  38. ncbi The glycosylation status of the murine hepatitis coronavirus M protein affects the interferogenic capacity of the virus in vitro and its ability to replicate in the liver but not the brain
    Cornelis A M de Haan
    Division of Virology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
    Virology 312:395-406. 2003
    ..This apparent correlation might be explained by the functioning of lectins, such as the mannose receptor, which are abundantly expressed in the liver but also play a role in the induction of interferon-alpha by dendritic cells...
  39. pmc Recombinant soluble, multimeric HA and NA exhibit distinctive types of protection against pandemic swine-origin 2009 A(H1N1) influenza virus infection in ferrets
    Berend Jan Bosch
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL, Utrecht, The Netherlands
    J Virol 84:10366-74. 2010
    ..Its inclusion in a vaccine is likely to reduce the HA dose required and to broaden the protective immunity...
  40. pmc The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex
    Berend Jan Bosch
    Virology Division, Department of Infectious Diseases and Immunity, Faculty of Veterinary Medicine, and Institute of Biomembranes, Utrecht University, 3584 CL Utrecht, The Netherlands
    J Virol 77:8801-11. 2003
    ..Both biochemical and functional data show that the coronavirus spike protein is a class I viral fusion protein...
  41. pmc Coronaviruses as vectors: stability of foreign gene expression
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht, The Netherlands
    J Virol 79:12742-51. 2005
    ..It also depended significantly on the particular genomic location at which the gene was inserted. The data indicate that foreign sequences are more stably maintained when replacing nonessential coronaviral genes...
  42. pmc Formation of disulfide-linked complexes between the three minor envelope glycoproteins (GP2b, GP3, and GP4) of equine arteritis virus
    Roeland Wieringa
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
    J Virol 77:6216-26. 2003
    ..Our study reveals that the minor envelope proteins of arteriviruses enter into both covalent and noncovalent interactions, the function of which has yet to be established...
  43. pmc Coronavirus escape from heptad repeat 2 (HR2)-derived peptide entry inhibition as a result of mutations in the HR1 domain of the spike fusion protein
    Berend Jan Bosch
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine, and Institute of Biomembranes, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 82:2580-5. 2008
    ....
  44. pmc Improved microarray gene expression profiling of virus-infected cells after removal of viral RNA
    Matthijs Raaben
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    BMC Genomics 9:221. 2008
    ..Yet, this effect has not been investigated despite the many reports that describe gene expression profiling of virus-infected cells using microarrays...
  45. pmc Evolution of the hemagglutinin protein of the new pandemic H1N1 influenza virus: maintaining optimal receptor binding by compensatory substitutions
    Robert P de Vries
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Virol 87:13868-77. 2013
    ..Moreover, these results are in agreement with a recent model for antigenic drift, in which influenza A virus mutants with high and low receptor-binding avidity alternate. ..
  46. pmc Glycan-dependent immunogenicity of recombinant soluble trimeric hemagglutinin
    Robert P de Vries
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
    J Virol 86:11735-44. 2012
    ..We conclude that the glycosylation state of recombinant antigens is a factor of significant importance when developing glycoprotein-based vaccines, such as recombinant HA proteins...
  47. pmc Mouse hepatitis coronavirus RNA replication depends on GBF1-mediated ARF1 activation
    Monique H Verheije
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
    PLoS Pathog 4:e1000088. 2008
    ..Collectively, our results demonstrate that GBF1-mediated ARF1 activation is required for efficient MHV RNA replication and reveal that the early secretory pathway and MHV replication complex formation are closely connected...
  48. ncbi Molecular interactions in the assembly of coronaviruses
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
    Adv Virus Res 64:165-230. 2005
  49. pmc Coronaviruses maintain viability despite dramatic rearrangements of the strictly conserved genome organization
    Cornelis A M de Haan
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, and Institute of Biomembranes, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol 76:12491-502. 2002
    ....
  50. ncbi A mRNA PCR for the diagnosis of feline infectious peritonitis
    Fermin A Simons
    Institute of Virology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Virol Methods 124:111-6. 2005
    ..In view of the low rate of false-positive results (high specificity) the mRNA RT-PCR may be a valuable addition to the diagnostic arsenal for FIP...
  51. pmc Only two residues are responsible for the dramatic difference in receptor binding between swine and new pandemic H1 hemagglutinin
    Robert P de Vries
    Division of Virology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht 3584 CL, The Netherlands
    J Biol Chem 286:5868-75. 2011
    ..Interestingly, whereas alanine at position 200 is found in most new pandemic human viruses, the residue at position 227 in these viruses is invariably a glutamic acid...
  52. ncbi Membrane rearrangements mediated by coronavirus nonstructural proteins 3 and 4
    Marne C Hagemeijer
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    Virology 458:125-35. 2014
    ....
  53. pmc Identification and characterization of a proteolytically primed form of the murine coronavirus spike proteins after fusion with the target cell
    Oliver Wicht
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Virol 88:4943-52. 2014
    ..The precise cleavage site and the enzymes involved remain to be elucidated...
  54. pmc Biogenesis and dynamics of the coronavirus replicative structures
    Marne C Hagemeijer
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    Viruses 4:3245-69. 2012
    ....
  55. pmc Visualizing coronavirus RNA synthesis in time by using click chemistry
    Marne C Hagemeijer
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
    J Virol 86:5808-16. 2012
    ..Rather, late in infection many DMVs may harbor dsRNA molecules that are no longer functioning as intermediates in RNA synthesis...
  56. ncbi Mouse hepatitis coronavirus replication induces host translational shutoff and mRNA decay, with concomitant formation of stress granules and processing bodies
    Matthijs Raaben
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    Cell Microbiol 9:2218-29. 2007
    ....
  57. ncbi Proteolytic activation of the porcine epidemic diarrhea coronavirus spike fusion protein by trypsin in cell culture
    Oliver Wicht
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Virol 88:7952-61. 2014
    ....
  58. pmc Coronavirus genetically redirected to the epidermal growth factor receptor exhibits effective antitumor activity against a malignant glioblastoma
    Monique H Verheije
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
    J Virol 83:7507-16. 2009
    ..This is the first demonstration of oncolytic activity of a coronavirus in vivo. It suggests that nonhuman coronaviruses may be attractive new therapeutic agents against human tumors...
  59. pmc Inhibition of the ubiquitin-proteasome system affects influenza A virus infection at a postfusion step
    Ivy Widjaja
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Virol 84:9625-31. 2010
    ..This was confirmed by the lack of inhibition of IAV entry by proteasome inhibitors in a virus-like particle fusion assay...
  60. ncbi Expression of epidermal growth factor receptor in canine osteosarcoma: association with clinicopathological parameters and prognosis
    Gayathri T Selvarajah
    Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 104, 3584CM Utrecht, The Netherlands
    Vet J 193:412-9. 2012
    ..However, these findings suggest that a subset of dogs may benefit from anti-EGFR adjuvant therapies...
  61. pmc Sequence analysis of feline coronaviruses and the circulating virulent/avirulent theory
    Hui Wen Chang
    Utrecht University, Utrecht, The Netherlands
    Emerg Infect Dis 17:744-6. 2011
    ..In contrast, FIPV occurs sporadically but is lethal. It replicates in monocytes and macrophages and rapidly disseminates throughout the body causing systemic immunopathologic disease (1-4)...
  62. ncbi The group-specific murine coronavirus genes are not essential, but their deletion, by reverse genetics, is attenuating in the natural host
    Cornelis A M de Haan
    Virology Division, Utrecht University, Utrecht, The Netherlands
    Virology 296:177-89. 2002
    ..Therefore, deletion of the coronavirus group-specific genes seems to provide an attractive approach to generate attenuated live coronavirus vaccines...
  63. ncbi Adverse effects of feline IL-12 during DNA vaccination against feline infectious peritonitis virus
    Harrie L Glansbeek
    Virology Division, Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
    J Gen Virol 83:1-10. 2002
    ..This study shows that DNA vaccination failed to protect cats against FIP and that IL-12 may yield adverse effects when used as a cytokine adjuvant...
  64. pmc Characterization of two new structural glycoproteins, GP(3) and GP(4), of equine arteritis virus
    Roeland Wieringa
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, and Institute of Biomembranes, Utrecht University, 3584 CL Utrecht, The Netherlands
    J Virol 76:10829-40. 2002
    ..Most, but not all, of the oligosaccharides of these virion glycoproteins are biochemically mature. Our results bring the number of EAV envelope proteins to six...
  65. ncbi Identification of host factors involved in coronavirus replication by quantitative proteomics analysis
    Mijke W Vogels
    Department of Biochemistry and Cell Biology, Biochemistry Division, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    Proteomics 11:64-80. 2011
    ..Overall, our study shows that the SILAC approach is a suitable tool to study host-pathogen interactions and to identify host proteins involved in virus replication...
  66. ncbi SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells
    I Chueh Huang
    Pulmonary Division, Children s Hospital, Children s Hospital Laboratory of Molecular Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:3198-203. 2006
    ..Our data indicate that two coronaviruses that utilize a common receptor nonetheless enter cells through distinct mechanisms...
  67. pmc Generation of a candidate live marker vaccine for equine arteritis virus by deletion of the major virus neutralization domain
    Javier Castillo-Olivares
    Centre for Preventive Medicine, Animal Health Trust, Kentford, Newmarket, Suffolk CB8 7UU, United Kingdom
    J Virol 77:8470-80. 2003
    ..EAV-G(L)Delta or similar mutants are therefore attractive marker vaccine candidates, enabling serological discrimination between vaccinated and wild-type virus-infected animals...
  68. ncbi The intraleader AUG nucleotide sequence context is important for equine arteritis virus replication
    Denis Archambault
    Department of Biological Sciences, University of Quebec at Montreal, Succursale Centre Ville, P O Box 8888, H3C 3P8, Montreal, Quebec, Canada
    Virus Genes 33:59-68. 2006
    ..As we discuss, the available data indicate that the ILO 5( nucleotide sequence per se, not its functioning in translation initiation, is of critical importance for EAV replication...
  69. pmc Role of endocytosis and low pH in murine hepatitis virus strain A59 cell entry
    Patricia Eifart
    Institut für Biologie Biophysik, Humboldt Universitat zu Berlin, Invalidenstr 42, D 10115 Berlin, Germany
    J Virol 81:10758-68. 2007
    ..The results imply that endocytosis plays a major role in MHV-A59 infection and the acidic pH of the endosomal compartment triggers a conformational change of the S protein mediating fusion...
  70. ncbi Homotypic interactions of the infectious bursal disease virus proteins VP3, pVP2, VP4, and VP5: mapping of the interacting domains
    Mirriam G J Tacken
    Institute for Animal Science and Health ID Lelystad B V, Division of Infectious Diseases and Food Chain Quality, P O Box 65, NL 8200 AB Lelystad, The Netherlands
    Virology 312:306-19. 2003
    ..Finally, one interaction domain was detected in the central, most hydrophobic region of VP5, supporting the idea that this virulence determinant may function as a membrane pore-forming protein in infected cells...
  71. ncbi Central ions and lateral asparagine/glutamine zippers stabilize the post-fusion hairpin conformation of the SARS coronavirus spike glycoprotein
    Stephane Duquerroy
    Laboratoire de Virologie Moléculaire and Structurale, UMR 2472 1157 CNRS INRA and IFR 115, 1 Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Virology 335:276-85. 2005
    ....
  72. pmc Infectious bursal disease virus capsid protein VP3 interacts both with VP1, the RNA-dependent RNA polymerase, and with viral double-stranded RNA
    Mirriam G J Tacken
    Division of Infectious Diseases and Food Chain Quality, Institute for Animal Science and Health ID Lelystad B V, NL 8200 AB Lelystad, The Netherlands
    J Virol 76:11301-11. 2002
    ..VP3 seems to be the key organizer of birnavirus structure, as it maintains critical interactions with all components of the viral particle: itself, VP2, VP1, and the two genomic dsRNAs...