H C Gerritsen

Summary

Affiliation: Utrecht University
Country: The Netherlands

Publications

  1. ncbi request reprint Imaging of optically thick specimen using two-photon excitation microscopy
    H C Gerritsen
    Debye Institute, Utrecht University, 3508 TA Utrecht, The Netherlands
    Microsc Res Tech 47:206-9. 1999
  2. ncbi request reprint Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution
    H C Gerritsen
    Debye Institute, Utrecht University, PO Box 80 000, NL 3508 TA, Utrecht, The Netherlands
    J Microsc 206:218-24. 2002
  3. ncbi request reprint Fast fluorescence lifetime imaging of calcium in living cells
    A V Agronskaia
    Utrecht University, Debye Institute, Department of Molecular Biophysics, P O Box 80000, 3508 TA Utrecht, The Netherlands
    J Biomed Opt 9:1230-7. 2004

Collaborators

  • A V Agronskaia
  • L Tertoolen

Detail Information

Publications3

  1. ncbi request reprint Imaging of optically thick specimen using two-photon excitation microscopy
    H C Gerritsen
    Debye Institute, Utrecht University, 3508 TA Utrecht, The Netherlands
    Microsc Res Tech 47:206-9. 1999
    ..The experiments carried out using the water immersion objective showed no significant degradation of both the axial and lateral resolution and the fluorescence intensity...
  2. ncbi request reprint Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution
    H C Gerritsen
    Debye Institute, Utrecht University, PO Box 80 000, NL 3508 TA, Utrecht, The Netherlands
    J Microsc 206:218-24. 2002
    ..Therefore, the acquisition speed with MCP-PMT based systems is limited. With a fast PMT operated close to its maximum count rate we were able to record a fluorescence lifetime image of a beating myocyte in less than one second...
  3. ncbi request reprint Fast fluorescence lifetime imaging of calcium in living cells
    A V Agronskaia
    Utrecht University, Debye Institute, Department of Molecular Biophysics, P O Box 80000, 3508 TA Utrecht, The Netherlands
    J Biomed Opt 9:1230-7. 2004
    ..The fast FLIM experiments on the myocytes could be carried out at a 100-Hz frame rate. During the beating of the myocytes a lifetime change of about 20% is observed. From the lifetime images a rest calcium level of about 65 nM is found...