- Imaging of optically thick specimen using two-photon excitation microscopyH C Gerritsen
Debye Institute, Utrecht University, 3508 TA Utrecht, The Netherlands
Microsc Res Tech 47:206-9. 1999..The experiments carried out using the water immersion objective showed no significant degradation of both the axial and lateral resolution and the fluorescence intensity...
- Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolutionH C Gerritsen
Debye Institute, Utrecht University, PO Box 80 000, NL 3508 TA, Utrecht, The Netherlands
J Microsc 206:218-24. 2002..Therefore, the acquisition speed with MCP-PMT based systems is limited. With a fast PMT operated close to its maximum count rate we were able to record a fluorescence lifetime image of a beating myocyte in less than one second...
- Fast fluorescence lifetime imaging of calcium in living cellsA V Agronskaia
Utrecht University, Debye Institute, Department of Molecular Biophysics, P O Box 80000, 3508 TA Utrecht, The Netherlands
J Biomed Opt 9:1230-7. 2004..The fast FLIM experiments on the myocytes could be carried out at a 100-Hz frame rate. During the beating of the myocytes a lifetime change of about 20% is observed. From the lifetime images a rest calcium level of about 65 nM is found...