Paul J van Diest

Summary

Affiliation: University Medical Center Utrecht
Country: The Netherlands

Publications

  1. pmc Loss of expression of FANCD2 protein in sporadic and hereditary breast cancer
    Petra van der Groep
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Breast Cancer Res Treat 107:41-7. 2008
  2. pmc Prognostic value of proliferation in invasive breast cancer: a review
    P J van Diest
    Department of Pathology, University Medical Centre Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    J Clin Pathol 57:675-81. 2004
  3. pmc The correlation between clinical, nuclear and histologic findings in a patient with Von Recklinghausen's disease
    Justus Martijn Brinkman
    Department of Nuclear Medicine and PET Research, VU University Medical Center, Amsterdam, The Netherlands
    World J Surg Oncol 5:130. 2007
  4. pmc HIF1-alpha overexpression indicates a good prognosis in early stage squamous cell carcinomas of the oral floor
    Thomas Fillies
    Department of Cranio Maxillofacial Surgery, University of Munster, Waldeyerstrasse 30, 48129 Munster, Germany
    BMC Cancer 5:84. 2005
  5. ncbi Why prostate tumour delineation based on apparent diffusion coefficient is challenging: an exploration of the tissue microanatomy
    Alie Borren
    Department of Radiotherapy, University Medical Center Utrecht, Utrecht, The Netherlands
    Acta Oncol 52:1629-36. 2013
  6. ncbi Analysis of copy number changes on chromosome 16q in male breast cancer by multiplex ligation-dependent probe amplification
    Miangela M Lacle
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Mod Pathol 26:1461-7. 2013
  7. pmc HIF-1α overexpression in ductal carcinoma in situ of the breast in BRCA1 and BRCA2 mutation carriers
    Petra van der Groep
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 8:e56055. 2013
  8. doi Fibrotic focus and hypoxia in male breast cancer
    Robert Kornegoor
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 25:1397-404. 2012
  9. doi Promoter hypermethylation patterns in fallopian tube epithelium of BRCA1 and BRCA2 germ line mutation carriers
    Jonathan G Bijron
    Department of Pathology, University Medical Centre Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Endocr Relat Cancer 19:69-81. 2012
  10. doi Genomic evolution from primary breast carcinoma to distant metastasis: Few copy number changes of breast cancer related genes
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands Electronic address
    Cancer Lett 344:138-46. 2014

Detail Information

Publications97

  1. pmc Loss of expression of FANCD2 protein in sporadic and hereditary breast cancer
    Petra van der Groep
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Breast Cancer Res Treat 107:41-7. 2008
    ..FANCD2 is of independent prognostic value in sporadic breast cancer...
  2. pmc Prognostic value of proliferation in invasive breast cancer: a review
    P J van Diest
    Department of Pathology, University Medical Centre Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    J Clin Pathol 57:675-81. 2004
    ..Mitosis counting provides the most reproducible and independent prognostic value, and Ki67/MIB1 labelling and cyclin A index are promising alternatives that need methodological fine tuning...
  3. pmc The correlation between clinical, nuclear and histologic findings in a patient with Von Recklinghausen's disease
    Justus Martijn Brinkman
    Department of Nuclear Medicine and PET Research, VU University Medical Center, Amsterdam, The Netherlands
    World J Surg Oncol 5:130. 2007
    ..abstract:..
  4. pmc HIF1-alpha overexpression indicates a good prognosis in early stage squamous cell carcinomas of the oral floor
    Thomas Fillies
    Department of Cranio Maxillofacial Surgery, University of Munster, Waldeyerstrasse 30, 48129 Munster, Germany
    BMC Cancer 5:84. 2005
    ..Therefore, HIF-1alpha expression in early stage oral carcinomas was evaluated in relation to established clinico-pathological features in order to determine its value as a prognostic marker...
  5. ncbi Why prostate tumour delineation based on apparent diffusion coefficient is challenging: an exploration of the tissue microanatomy
    Alie Borren
    Department of Radiotherapy, University Medical Center Utrecht, Utrecht, The Netherlands
    Acta Oncol 52:1629-36. 2013
    ..Therefore, in this study the relationship between ADC and histological variables was investigated on voxel level to understand the strengths and limitations of DWI for prostate tumour delineation...
  6. ncbi Analysis of copy number changes on chromosome 16q in male breast cancer by multiplex ligation-dependent probe amplification
    Miangela M Lacle
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Mod Pathol 26:1461-7. 2013
    ..Gain on 16p and loss of 16q identify a group of male breast cancer with low propensity to develop lymph node metastases. ..
  7. pmc HIF-1α overexpression in ductal carcinoma in situ of the breast in BRCA1 and BRCA2 mutation carriers
    Petra van der Groep
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 8:e56055. 2013
    ..Hypoxia-related proteins are therefore putative targets for therapy and molecular imaging for early detection and monitoring therapy response in BRCA mutation patients...
  8. doi Fibrotic focus and hypoxia in male breast cancer
    Robert Kornegoor
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 25:1397-404. 2012
    ..These markers seem to have similar clinical importance as previously reported in female breast cancer...
  9. doi Promoter hypermethylation patterns in fallopian tube epithelium of BRCA1 and BRCA2 germ line mutation carriers
    Jonathan G Bijron
    Department of Pathology, University Medical Centre Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Endocr Relat Cancer 19:69-81. 2012
    ..Methylation could, therefore, play an important role in the increased risk of gynaecological malignancies in BRCA mutation carriers...
  10. doi Genomic evolution from primary breast carcinoma to distant metastasis: Few copy number changes of breast cancer related genes
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands Electronic address
    Cancer Lett 344:138-46. 2014
    ..The few genes that showed differences between primary tumor and metastasis (PRDM14, MED1, CCNE1, TRAF4, MTDH, CDH1) have been implicated in the development of therapy resistance...
  11. ncbi Discrimination between benign and malignant prostate tissue using chromatin texture analysis in 3-D by confocal laser scanning microscopy
    André Huisman
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Prostate 67:248-54. 2007
    ..Confocal laser scanning microscopy (CLSM) allows performing measurements in nuclei reconstructed in 3-D. The aim of this study was to evaluate the clinical usefulness of 3-D texture analysis of prostate tissue...
  12. doi Accelerated perinecrotic outgrowth of colorectal liver metastases following radiofrequency ablation is a hypoxia-driven phenomenon
    Maarten W Nijkamp
    Department of Surgery, University Medical Center Utrecht, Utrecht, The Netherlands
    Ann Surg 249:814-23. 2009
    ....
  13. doi Molecular profiling of invasive breast cancer by multiplex ligation-dependent probe amplification-based copy number analysis of tumor suppressor and oncogenes
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Mod Pathol 23:1029-39. 2010
    ....
  14. doi Expression of hypoxia-inducible factor-1α and -2α in whole-mount prostate histology: relation with dynamic contrast-enhanced MRI and Gleason score
    Alie Borren
    Department of Radiotherapy, University Medical Center Utrecht, Utrecht, The Netherlands
    Oncol Rep 29:2249-54. 2013
    ..However, HIF-1α and HIF-2α were not correlated with DCE-MRI parameters. Given the pronounced expression of HIF-2α and independence of Gleason score, HIF expression may function as a biomarker to guide boost dose prescription...
  15. pmc Exploring gene expression signatures for predicting disease free survival after resection of colorectal cancer liver metastases
    Nikol Snoeren
    Department of Surgery, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, The Netherlands
    PLoS ONE 7:e49442. 2012
    ..This study was designed to identify and validate gene signatures that can predict disease free survival (DFS) in patients undergoing a radical resection for their colorectal liver metastases (CRLM)...
  16. doi Molecular imaging with a fluorescent antibody targeting carbonic anhydrase IX can successfully detect hypoxic ductal carcinoma in situ of the breast
    Aram S A van Brussel
    Division of Cell Biology, Department of Biology, Science Faculty, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    Breast Cancer Res Treat 140:263-72. 2013
    ..Furthermore, it allows for sensitive CAIX-specific immunofluorescence microscopy of tumor sections, thereby introducing the concept of molecular fluorescence pathology. ..
  17. pmc Oncogene amplification in male breast cancer: analysis by multiplex ligation-dependent probe amplification
    Robert Kornegoor
    Department of Pathology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Breast Cancer Res Treat 135:49-58. 2012
    ..In addition CCND1 amplification seems to be an independent prognosticator in male breast cancer...
  18. pmc Receptor conversion in distant breast cancer metastases
    Laurien D C Hoefnagel
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    Breast Cancer Res 12:R75. 2010
    ....
  19. doi Progression risk of columnar cell lesions of the breast diagnosed in core needle biopsies
    Anoek H J Verschuur-Maes
    Department of Pathology, University Medical Centre Utrecht, The Netherlands
    Int J Cancer 129:2674-80. 2011
    ..This illustrates that an atypical CCL in a CNB may signal the presence of concurrent lesions or development of advanced lesions in future and may justify ("mini") surgical excision...
  20. doi Accuracy of sentinel node biopsy after neoadjuvant chemotherapy in breast cancer patients: a systematic review
    Carolien H M van Deurzen
    Department of Pathology, University Medical Centre Utrecht, The Netherlands
    Eur J Cancer 45:3124-30. 2009
    ..This review was conducted to determine the accuracy of SN biopsy following NAC...
  21. doi Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3
    Arjan J Groot
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Mol Immunol 46:2022-8. 2009
    ..Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment...
  22. ncbi Levels of hypoxia-inducible factor-1alpha independently predict prognosis in patients with lymph node negative breast carcinoma
    Reinhard Bos
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Cancer 97:1573-81. 2003
    ..In this study, the prognostic value of HIF-1alpha expression and its correlation with various clinicopathologic variables in patients with invasive breast carcinoma were investigated...
  23. ncbi High frequency of HIF-1alpha overexpression in BRCA1 related breast cancer
    Petra van der Groep
    Department of Pathology, University Medical Center, 3508 GA, Utrecht, The Netherlands
    Breast Cancer Res Treat 111:475-80. 2008
    ..This points to an important role of hypoxia and its key regulator HIF-1alpha in hereditary breast carcinogenesis...
  24. pmc Nuclear Kaiso expression is associated with high grade and triple-negative invasive breast cancer
    Jeroen F Vermeulen
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 7:e37864. 2012
    ..In conclusion, our data indicate that nuclear Kaiso is common in clinically aggressive ductal breast cancer, while cytoplasmic Kaiso and a p120-mediated relief of Kaiso-dependent transcriptional repression characterize ILC...
  25. pmc Non-sentinel lymph node metastases associated with isolated breast cancer cells in the sentinel node
    Carolien H M van Deurzen
    Department of Pathology, University Medical Center Utrecht, GA Utrecht, The Netherlands
    J Natl Cancer Inst 100:1574-80. 2008
    ....
  26. doi Simultaneous detection of TOP2A and HER2 gene amplification by multiplex ligation-dependent probe amplification in breast cancer
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 23:62-70. 2010
    ..In conclusion, MLPA is an easy and accurate method to simultaneously detect breast cancer HER-2/neu and TopoII alpha copy number status in paraffin-embedded tissue, and thus an attractive supplement or alternative to CISH...
  27. ncbi Methylation of the TWIST1 promoter, TWIST1 mRNA levels, and immunohistochemical expression of TWIST1 in breast cancer
    Eelke H Gort
    Department of Pathology, University Medical Center Utrecht, P O Box 85500, 3508 GA Utrecht, The Netherlands
    Cancer Epidemiol Biomarkers Prev 17:3325-30. 2008
    ..This indicates that although TWIST1 CpG methylation is useful as a biomarker in breast cancer diagnosis, there is no direct correlation with TWIST1 expression...
  28. pmc The potential of hypoxia markers as target for breast molecular imaging--a systematic review and meta-analysis of human marker expression
    Arthur Adams
    Department of Radiology, University Medical Center Utrecht, Utrecht, The Netherlands
    BMC Cancer 13:538. 2013
    ..We performed a systematic literature review and meta-analysis of such hypoxia marker expression rates in human breast cancer to evaluate their potential as clinically relevant molecular imaging targets...
  29. doi Morphometry of isolated tumor cells in breast cancer sentinel lymph nodes: metastases or displacement?
    Carolien H M van Deurzen
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    Am J Surg Pathol 33:106-10. 2009
    ..This supports the hypothesis that some of these deposits could represent benign epithelium or degenerated malignant cells lacking outgrowth potential...
  30. pmc Perinecrotic hypoxia contributes to ischemia/reperfusion-accelerated outgrowth of colorectal micrometastases
    Jarmila D W van der Bilt
    Department of Surgery G04 228, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Am J Pathol 170:1379-88. 2007
    ..Prevention of tissue hypoxia or inhibition of HIF-1alpha may represent attractive approaches to limiting recurrent tumor growth after hepatic surgery...
  31. doi Radiofrequency ablation of colorectal liver metastases induces an inflammatory response in distant hepatic metastases but not in local accelerated outgrowth
    Maarten W Nijkamp
    Department of Surgery, University Medical Center Utrecht, Utrecht, The Netherlands
    J Surg Oncol 101:551-6. 2010
    ..Here we have evaluated whether an altered density of inflammatory cells could be observed in the perinecrotic (TZ) metastases compared to hepatic metastases in the distant reference zone (RZ)...
  32. ncbi No amplifications of hypoxia-inducible factor-1alpha gene in invasive breast cancer: a tissue microarray study
    Marije M Vleugel
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Cell Oncol 26:347-51. 2004
    ..The aim of this study was to evaluate the presence of HIF-1alpha gene amplifications in invasive breast cancer as an explanation for HIF-1alpha protein overexpression...
  33. ncbi The invasive front in endometrial carcinoma: higher proliferation and associated derailment of cell cycle regulators
    Nicole Horrée
    Department of Surgical Gynecology and Oncology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Hum Pathol 38:1232-8. 2007
    ..Endometrial carcinomas clearly show an invasive front that is characterized by higher proliferation and progressive derailment of the cell cycle regulators cyclin E, p16, and cdk2, but not by an increased hypoxic response...
  34. doi Fallopian tube intraluminal tumor spread from noninvasive precursor lesions: a novel metastatic route in early pelvic carcinogenesis
    Jonathan G Bijron
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Am J Surg Pathol 37:1123-30. 2013
    ..Although rare in the absence of invasive cancer, we show that intraluminal shedding of tumor cells in the fallopian tubes from serous carcinoma cases are common and a likely route of abdominal spread. ..
  35. ncbi Discrimination between benign and malignant prostate biopsies using three-dimensional chromatin texture analysis by confocal laser scanning microscopy
    André Huisman
    Department of Pathology, University Medical Center Utrecht, P O Box 85500, 3508 GA Utrecht, The Netherlands
    Anal Quant Cytol Histol 33:265-70. 2011
    ..To evaluate the clinical usefulness of computing three-dimensional (3-D) nuclear texture features on prostate biopsy specimens to discriminate among benign, prostatic intraepithelial neoplasia (PIN), and malignant specimens...
  36. pmc Expression of hypoxia-inducible factor-1alpha and cell cycle proteins in invasive breast cancer are estrogen receptor related
    Reinhard Bos
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Breast Cancer Res 6:R450-9. 2004
    ..In this study we undertook a detailed evaluation of the relationships between HIF-1alpha and cell cycle-associated proteins...
  37. pmc Necrosis related HIF-1alpha expression predicts prognosis in patients with endometrioid endometrial carcinoma
    Laura M S Seeber
    Department of Gynaecological Oncology, University Medical Centre Utrecht, The Netherlands
    BMC Cancer 10:307. 2010
    ..This possibly contributes to survival of cancer cells. The aim of this study was to evaluate the prognostic value of HIF-1alpha and p27kip expression in patients with endometrioid endometrial cancer...
  38. doi Amplification testing in breast cancer by multiplex ligation-dependent probe amplification of microdissected tissue
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Methods Mol Biol 755:107-18. 2011
    ..This chapter describes a method for MLPA analysis using invasive breast tumor cells acquired by laser capture microdissection. This protocol can also be applied to MLPA analysis of preinvasive lesions and metastases...
  39. ncbi Development of 3D chromatin texture analysis using confocal laser scanning microscopy
    André Huisman
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cell Oncol 27:335-45. 2005
    ..The aim of this study was to develop 3D texture features that quantitatively describe changes in chromatin architecture associated with malignancy using CLSM images...
  40. ncbi Protein expression of B-cell lymphoma gene 6 (BCL-6) in invasive breast cancer is associated with cyclin D1 and hypoxia-inducible factor-1alpha (HIF-1alpha)
    Reinhard Bos
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Oncogene 22:8948-51. 2003
    ..The cases with amplification in BCL-6 showed an increased (25%) incidence of BCL-6 protein overexpression. Thus, this study is the first to show that BCL-6 oncogene activation plays a role in cancers other than lymphomas...
  41. doi Loss of p120-catenin induces metastatic progression of breast cancer by inducing anoikis resistance and augmenting growth factor receptor signaling
    Ron C J Schackmann
    Department of Pathology, Cancer Center, and Division of Internal Medicine, University Medical Center Utrecht, Utrecht, The Netherlands
    Cancer Res 73:4937-49. 2013
    ..Our results establish a preclinical platform to develop tailored intervention regimens that target GFR signals to treat p120-negative metastatic breast cancers...
  42. doi Frequent promoter hypermethylation of BRCA2, CDH13, MSH6, PAX5, PAX6 and WT1 in ductal carcinoma in situ and invasive breast cancer
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Heidelberglaan 100, Utrecht, The Netherlands
    J Pathol 225:222-31. 2011
    ..Promoter hypermethylation of PAX6, BRCA2, PAX5, WT1, CDH13 and MSH6 seems to be a frequent early event in breast cancer and methylation levels of GSTP1 (and CDKN2A, although still low) seem to increase with increasing DCIS grade...
  43. ncbi c-Jun activation is associated with proliferation and angiogenesis in invasive breast cancer
    Marije M Vleugel
    Department of Pathology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Hum Pathol 37:668-74. 2006
    ..Our present results in breast cancer patients confirm this relation in vivo for the first time. Therefore, c-Jun/AP-1 targeting may provide new ways to block tumor angiogenesis...
  44. ncbi FANCD2 protein is expressed in proliferating cells of human tissues that are cancer-prone in Fanconi anaemia
    Michael Hölzel
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    J Pathol 201:198-203. 2003
    ..FANCD2 expression is frequently expressed in proliferating cells as demonstrated by Ki-67 immunofluorescence double staining, consistent with a function of FANCD2 in DNA replication...
  45. doi Immunophenotyping of male breast cancer
    Robert Kornegoor
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Histopathology 61:1145-55. 2012
    ..Male breast cancer is a rare disease, and knowledge of carcinogenesis is limited. Conflicting results, based on small series, have been reported for clinically relevant biomarkers...
  46. doi Molecular subtyping of male breast cancer by immunohistochemistry
    Robert Kornegoor
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 25:398-404. 2012
    ..This distribution of molecular subtypes in male breast cancer is clearly different compared with female breast cancers, pointing to possible important differences in carcinogenesis...
  47. doi Whole slide images for primary diagnostics of gastrointestinal tract pathology: a feasibility study
    Shaimaa Al-Janabi
    Department of Pathology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Hum Pathol 43:702-7. 2012
    ..Up-front histopathologic diagnosis of gastrointestinal biopsies and resections can be done on whole slide images...
  48. pmc Expression of the stem cell marker ALDH1 in BRCA1 related breast cancer
    Marise R Heerma van Voss
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Cell Oncol (Dordr) 34:3-10. 2011
    ..Since ALDH1 is a marker of both mammary stem cells and breast cancer stem cells, we compared ALDH1 expression in malignant tissue of BRCA1 mutation carriers to non-carriers...
  49. doi Columnar cell lesions on breast needle biopsies: is surgical excision necessary? A systematic review
    Anoek H J Verschuur-Maes
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    Ann Surg 255:259-65. 2012
    ..This systematic review was conducted to provide treatment recommendations for patients with a diagnosis of columnar cell lesions (CCLs) in a breast core needle biopsy (CNB)...
  50. ncbi Conditional inactivation of HIF-1 using intrabodies
    Arjan J Groot
    Department of Pathology, University Medical Center Utrecht, 3508 GA, Utrecht, The Netherlands
    Cell Oncol 30:397-409. 2008
    ..Inducible intrabody targeting is a highly specific strategy for temporal protein inactivation and may have applications for disease treatment...
  51. doi Prognostic value of automatically extracted nuclear morphometric features in whole slide images of male breast cancer
    Mitko Veta
    Image Sciences Institute, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 25:1559-65. 2012
    ..With the increasing availability of slide scanning equipment in pathology labs, these kinds of quantitative approaches can be easily integrated in the workflow of routine pathology practice...
  52. pmc COMMD1 Promotes pVHL and O2-Independent Proteolysis of HIF-1alpha via HSP90/70
    Bart van De Sluis
    Complex Genetics Section, DBG Department of Medical Genetics, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 4:e7332. 2009
    ..Although HIF activity is mainly controlled by ubiquitination and protein degradation by the von Hippel Lindau (pVHL) tumor suppressor gene other mechanisms have recently been identified that regulate HIF signaling independently of pVHL...
  53. pmc Cytosolic p120-catenin regulates growth of metastatic lobular carcinoma through Rock1-mediated anoikis resistance
    Ron C J Schackmann
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Invest 121:3176-88. 2011
    ....
  54. pmc Near-infrared fluorescence molecular imaging of ductal carcinoma in situ with CD44v6-specific antibodies in mice: a preclinical study
    Jeroen F Vermeulen
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA, Utrecht, The Netherlands
    Mol Imaging Biol 15:290-8. 2013
    ..As CD44v6 is frequently expressed in DCIS, we used near-infrared fluorescently labeled CD44v6-targeting antibodies for detection of DCIS...
  55. pmc Poorly differentiated breast carcinoma is associated with increased expression of the human polycomb group EZH2 gene
    Frank M Raaphorst
    Department of Pathology, VU University Medical Center, BioCentrum Amsterdam, University of Amsterdam, Amsterdam, The Netherlands
    Neoplasia 5:481-8. 2003
    ..These observations suggest that deregulated expression of EZH2 is associated with loss of differentiation and development of poorly differentiated breast cancer in humans...
  56. doi Nodal-stage classification in invasive lobular breast carcinoma: influence of different interpretations of the pTNM classification
    Carolien H M van Deurzen
    University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    J Clin Oncol 28:999-1004. 2010
    ..Therefore, we suggest using the EWGBSP definitions in these patients to select high-risk patients who may benefit from additional local and/or systemic therapy...
  57. ncbi Implementation of accurate and fast DNA cytometry by confocal microscopy in 3D
    Lennert S Ploeger
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cell Oncol 27:225-30. 2005
    ..The aim of this study was to describe the conditions for accurate and fast 3D CLSM cytometry with a minimum of user interaction to arrive at sufficient throughput for pilot clinical applications...
  58. ncbi Mutation analysis of the HIF-1alpha oxygen-dependent degradation domain in invasive breast cancer
    Marije M Vleugel
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Cancer Genet Cytogenet 163:168-72. 2005
    ..No mutations seem to occur in the ODD of hif-1alpha in HIF-1alpha overexpressing invasive breast cancer, which rules ODD mutations out as a possible explanation for the diffuse HIF-1alpha expression pattern often seen in this cancer...
  59. doi Methylation profiles of endometrioid and serous endometrial cancers
    Laura M S Seeber
    Department of Gynaecological Oncology, University Medical Centre Utrecht, 3508 GA Utrecht, The Netherlands
    Endocr Relat Cancer 17:663-73. 2010
    ..A panel of methylation biomarkers could be useful to distinguish between the two histological subtypes of endometrial cancer. Furthermore, methylation of MLH1 may have prognostic value in EEC...
  60. pmc Expression of the RNA helicase DDX3 and the hypoxia response in breast cancer
    Guus M Bol
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    PLoS ONE 8:e63548. 2013
    ..However, the relation between DDX3 and hypoxia has not been addressed in human tumors. In this paper, we studied the relation between DDX3 and the hypoxic responsive proteins in human breast cancer...
  61. pmc Differential expression of growth factor receptors and membrane-bound tumor markers for imaging in male and female breast cancer
    Jeroen F Vermeulen
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 8:e53353. 2013
    ..We therefore investigated expression profiles of growth factor receptors and membrane-bound tumor markers in male breast cancer and gynecomastia, in comparison with female breast cancer...
  62. pmc Immunophenotyping invasive breast cancer: paving the road for molecular imaging
    Jeroen F Vermeulen
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    BMC Cancer 12:240. 2012
    ..Because development of new tracers is labor-intensive and costly, we searched for the smallest panel of tumor membrane markers that would allow detection of the wide spectrum of invasive breast cancers...
  63. doi Pathology issues related to SN procedures and increased detection of micrometastases and isolated tumor cells
    Paul J van Diest
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    Breast Dis 31:65-81. 2010
    ..In this paper, we provide an up to date discussion on the virtues and flaws of these different methods to find SN metastases, and provide recommendations on the optimal pathology protocol for breast cancer SNs...
  64. pmc Lympho-vascular invasion in BRCA related breast cancer compared to sporadic controls
    Marise R Heerma van Voss
    Department of Pathology, University Medical Center Utrecht, The Netherlands
    BMC Cancer 10:145. 2010
    ....
  65. doi ESR1 amplification is rare in breast cancer and is associated with high grade and high proliferation: a multiplex ligation-dependent probe amplification study
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Anal Cell Pathol (Amst) 33:13-8. 2010
    ....
  66. doi HIF-1α and NOTCH signaling in ductal and lobular carcinomas of the breast
    Cigdem Ercan
    Department of Pathology, University Medical Center Utrecht Cancer Center, Utrecht, The Netherlands
    Cell Oncol (Dordr) 35:435-42. 2012
    ..Hypoxia-induced stabilization of HIF transcription factors has been shown to lead to NOTCH activation in vitro in different contexts and tissues, causing differentiation arrest and induction of proliferation and migration...
  67. ncbi Whole slide images for primary diagnostics of paediatric pathology specimens: a feasibility study
    Shaimaa Al-Janabi
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Pathol 66:218-23. 2013
    ..The aim of this study was to test the feasibility of using WSI in primary diagnostics of paediatric pathology specimens and placental tissue...
  68. doi Epigenetic progression of columnar cell lesions of the breast to invasive breast cancer
    Anoek H J Verschuur-Maes
    Department of Pathology, University Medical Center Utrecht Cancer Center, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Breast Cancer Res Treat 136:705-15. 2012
    ..Atypia in CCL or the presence of synchronous more advanced lesions does not seem to be accompanied by higher methylation levels...
  69. ncbi Hypoxic regulation of metastasis via hypoxia-inducible factors
    Eelke H Gort
    Department of Pathology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Curr Mol Med 8:60-7. 2008
    ..This polyclonal hypoxic gene profile potentially develops into a metastatic profile, driving metastasis formation. The hypoxic gene profile in primary tumors may therefore provide a prognostic indicator in clinical decision-making...
  70. ncbi HER-2/neu amplification testing in breast cancer by multiplex ligation-dependent probe amplification in comparison with immunohistochemistry and in situ hybridization
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Heidelberglaan 100, Utrecht, The Netherlands
    Cell Oncol 31:1-10. 2009
    ..This study aimed to validate a new PCR based test, called Multiplex Ligation-dependent Probe Amplification (MLPA), as a simple and quick method to assess HER-2/neu gene amplification status in invasive breast cancer...
  71. pmc Expression of BNIP3 in invasive breast cancer: correlations with the hypoxic response and clinicopathological features
    Esther A Koop
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    BMC Cancer 9:175. 2009
    ..The aim of the present study was to investigate the expression of BNIP3 in invasive breast cancer at the mRNA and protein level in correlation with the hypoxic response and clinicopathological features...
  72. doi Isolated tumor cells in breast cancer sentinel lymph nodes: displacement or metastases? An immunohistochemical study
    Carolien H M van Deurzen
    Department of Pathology, University Medical Centre Utrecht, The Netherlands
    Hum Pathol 40:778-82. 2009
    ..This supports the hypothesis that some of these cells may be displaced benign cells or concern tumor cells with limited malignant potential compared with micro- and macrometastases...
  73. pmc Successful oxytocin-assisted nipple aspiration in women at increased risk for breast cancer
    Karijn P M Suijkerbuijk
    Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA, Utrecht, The Netherlands
    Fam Cancer 9:321-5. 2010
    ..6 (on a 0-10 scale), which was significantly lower than for mammography or MRI. These findings suggest that a new tool for biomarker detection in oxytocin-assisted nipple fluid of women at high risk for breast cancer is at hand...
  74. ncbi Genomic profiling by array comparative genomic hybridization reveals novel DNA copy number changes in breast phyllodes tumours
    Arno Kuijper
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Cell Oncol 31:31-9. 2009
    ..Since copy number change was found in all benign PT, genomic instability may be an early event in PT genesis...
  75. pmc Pathology of hereditary breast cancer
    Petra van der Groep
    Department of Pathology, University Medical Center Utrecht, P O Box 85500, 3508 GA, Utrecht, The Netherlands
    Cell Oncol (Dordr) 34:71-88. 2011
    ..However, in still a part of familial hereditary breast cancers no relationship to any of these breast cancer susceptibility genes can be found. Research on new susceptibility genes is therefore ongoing...
  76. pmc Low frequency of HER2 amplification and overexpression in early onset gastric cancer
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Heidelberglaan 100, PO Box 85500, 3508GA, Utrecht, The Netherlands
    Cell Oncol (Dordr) 34:89-95. 2011
    ..In light of potential trastuzumab benefit, subpopulations of GC such as EOGC (versus late onset) should be evaluated for their frequency of amplification and overexpression using currently available techniques...
  77. pmc Expression of hypoxia-inducible factor 1 alpha and its downstream targets in fibroepithelial tumors of the breast
    Arno Kuijper
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Breast Cancer Res 7:R808-18. 2005
    ..We studied the expression and prognostic relevance of HIF-1alpha and its downstream targets in phyllodes tumors and fibroadenomas of the breast...
  78. pmc p53 mutations in classic and pleomorphic invasive lobular carcinoma of the breast
    Cigdem Ercan
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cell Oncol (Dordr) 35:111-8. 2012
    ....
  79. pmc Subcellular FIH-1 expression patterns in invasive breast cancer in relation to HIF-1α expression
    Agon Hyseni
    Department of Pathology, University Medical Centre Utrecht, The Netherlands
    Cell Oncol (Dordr) 34:565-70. 2011
    ....
  80. doi Prognostic value of estrogen receptor α and progesterone receptor conversion in distant breast cancer metastases
    Laurien D C Hoefnagel
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cancer 118:4929-35. 2012
    ..The purpose of this study was to evaluate the impact of receptor conversion for ERα and PR on survival in a large group of distant non-bone breast cancer metastases...
  81. doi No non-sentinel node involvement in melanoma patients with limited Breslow thickness and low sentinel node tumour load
    Thomas Bogenrieder
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Histopathology 59:318-26. 2011
    ..The aim of the present study was to predict the absence of non-SN metastases in a multicentre study of patients with a positive SN based on primary melanoma features and SN tumour load...
  82. ncbi The prognostic value of proliferation in lymph-node-negative breast cancer patients is age dependent
    Jan P A Baak
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands, and Department of Pathology, Stavanger University Hospital, P O Box 8100, 4068 Stavanger, Norway
    Eur J Cancer 43:527-35. 2007
    ..006) for patients aged 55-70 years, while over 70 years, MAI was not significant (P=.11). The prognostic value of proliferation was age-dependent. Prognostic breast cancer studies must clearly indicate the age group being studied...
  83. doi Molecular differences between ductal carcinoma in situ and adjacent invasive breast carcinoma: a multiplex ligation-dependent probe amplification study
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Anal Cell Pathol (Amst) 33:165-73. 2010
    ..These high-grade DCIS specific genes may be potential targets for treatment and/or predict progression...
  84. ncbi A restaining method to restore faded fluorescence in tissue specimens for quantitative confocal microscopy
    André Huisman
    Department of Pathology, University Medical Center, Utrecht, The Netherlands
    Cytometry A 71:875-81. 2007
    ..With the described protocol it is possible to remove TO-PRO-3 fluorescence from tissue sections that can successfully be restained with minimal influence on fluorescence intensity and nuclear chromatin distribution...
  85. pmc HER-2/neu amplification testing in breast cancer by Multiplex Ligation-dependent Probe Amplification: influence of manual- and laser microdissection
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Heidelberglaan, GA Utrecht, The Netherlands
    BMC Cancer 9:4. 2009
    ..MLPA was previously shown to correlate well with IHC and in situ hybridization (ISH), but a low tumor percentage in the tissue tested could negatively affect the accuracy of MLPA results...
  86. doi Formaldehyde substitute fixatives: effects on nucleic acid preservation
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Pathol 64:960-7. 2011
    ..However, the commonly used formalin fixative is carcinogenic, and its crosslinking impairs DNA and RNA quality...
  87. doi Comparison of different promoter methylation assays in breast cancer
    Karijn P M Suijkerbuijk
    Department of Pathology, University Medical Center Utrecht Cancer Center, Utrecht, The Netherlands
    Anal Cell Pathol (Amst) 33:133-41. 2010
    ..Here we directly compared three commonly used methylation assays and assessed the influence of tissue fixation, target sequence location and the amount of DNA on their performance...
  88. ncbi p300 and p53 levels determine activation of HIF-1 downstream targets in invasive breast cancer
    Marije M Vleugel
    Department of Pathology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Hum Pathol 37:1085-92. 2006
    ..This underlines the importance of p300 levels and p53 accumulation in the HIF-1-regulated response toward hypoxia...
  89. doi Longitudinal 3.0T MRI analysis of changes in lymph node volume and apparent diffusion coefficient in an experimental animal model of metastatic and hyperplastic lymph nodes
    Wenche M Klerkx
    Department of Gynaecology and Obstetrics, University Medical Centre Utrecht, The Netherlands
    J Magn Reson Imaging 33:1151-9. 2011
    ..To perform a longitudinal analysis of changes in lymph node volume and apparent diffusion coefficient (ADC) in healthy, metastatic, and hyperplastic lymph nodes...
  90. pmc ESR1 amplification is rare in breast cancer and is associated with high grade and high proliferation: a multiplex ligation-dependent probe amplification study
    Cathy B Moelans
    Department of Pathology H04 312, University Medical Centre Utrecht, Heidelberglaan 100, PO Box 85500, 3508, GA, Utrecht, The Netherlands
    Cell Oncol (Dordr) 34:489-94. 2011
    ....
  91. ncbi Progressive deregulation of the cell cycle with higher tumor grade in the stroma of breast phyllodes tumors
    Arno Kuijper
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Am J Clin Pathol 123:690-8. 2005
    ..Several combinations of aberrantly expressed cell cycle proteins seem important in the stromal progression of phyllodes tumors. The number of stromal cell cycle aberrations and stromal p53 expression might predict clinical behavior...
  92. doi The peptidyl-isomerase Pin1 regulates p27kip1 expression through inhibition of Forkhead box O tumor suppressors
    Arjan B Brenkman
    Department of Metabolic and Endocrine Diseases and Netherlands Metabolomics Centre, University Medical Centre Utrecht, Utrecht, The Netherlands
    Cancer Res 68:7597-605. 2008
    ..Thus, Pin1 is identified as a novel negative FOXO regulator, interconnecting FOXO phosphorylation and monoubiquitination in response to cellular stress to regulate p27(kip1)...
  93. doi Validation of a fully automated HER2 staining kit in breast cancer
    Cathy B Moelans
    Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Cell Oncol 32:149-55. 2010
    ..Analytical variability in HER2 IHC testing could be diminished by a fully automatic staining system with a monoclonal antibody...
  94. doi Methylation profiles of hereditary and sporadic ovarian cancer
    Guus M Bol
    Department of Pathology, University Medical Centre Utrecht Cancer Centre, Utrecht, The Netherlands
    Histopathology 57:363-70. 2010
    ..The aim of this study was to investigate promoter methylation of a set of common tumour suppressor genes in BRCA1-related ovarian cancer in comparison with sporadic ovarian cancer...
  95. ncbi HIF-1 alpha expression is associated with an atheromatous inflammatory plaque phenotype and upregulated in activated macrophages
    Aryan Vink
    Department of Pathology, University Medical Center Utrecht, Ultrecht, The Netherlands
    Atherosclerosis 195:e69-75. 2007
    ..The transcription factor hypoxia-inducible factor-1 alpha (HIF-1 alpha) is a key regulator of angiogenesis and is also involved in inflammatory reactions. We studied HIF-1 alpha expression in different atherosclerotic plaque phenotypes...
  96. doi Whole slide images for primary diagnostics in dermatopathology: a feasibility study
    Shaimaa Al-Janabi
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Pathol 65:152-8. 2012
    ..However, WSI have not regularly been used for routine diagnosis, because of the lack of validation studies...
  97. ncbi Identification by phage display of single-domain antibody fragments specific for the ODD domain in hypoxia-inducible factor 1alpha
    Arjan J Groot
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Lab Invest 86:345-56. 2006
    ..These are the first single-domain antibody fragments that may be used in exploration of HIF-1alpha as a possible therapeutic target through molecular applications...