Research Topics
Genomes and Genes | Bert PoolmanSummaryAffiliation: University of Groningen Country: The Netherlands Publications
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Publications
Lateral diffusion of membrane proteinsSivaramakrishnan Ramadurai
Department of Biochemistry, Groningen Biomolecular science and Biotechnology Institute and Zernike Institute of Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Am Chem Soc 131:12650-6. 2009....
Bacterial osmosensing: roles of membrane structure and electrostatics in lipid-protein and protein-protein interactionsBert Poolman
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology and Materials Science Center plus, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Biochim Biophys Acta 1666:88-104. 2004..7 mV for monovalent ions. The osmosensing mechanism is qualitatively described as an "ON/OFF switch" representing thermally relaxed and electrostatically locked protein conformations...- How do membrane proteins sense water stress?Bert Poolman
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh, Groningen, The Netherlands
Mol Microbiol 44:889-902. 2002.... - Transporters and their roles in LAB cell physiologyBert Poolman
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Antonie Van Leeuwenhoek 82:147-64. 2002..The mechanosensitive channels have been best studied in organisms other than LAB, but the presence of similar systems in LAB, and their conservation of structure, suggest that the postulated functions and mechanisms generally hold... - Substrate-induced conformational changes in the membrane-embedded IIC(mtl)-domain of the mannitol permease from Escherichia coli, EnzymeII(mtl), probed by tryptophan phosphorescence spectroscopyGertjan Veldhuis
Department of Biochemistry and Biophysical Chemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Biol Chem 280:35148-56. 2005..The observations are discussed in context of the current mechanistic and structural model of EII(mtl)... 
Stoichiometry and substrate affinity of the mannitol transporter, EnzymeIImtl, from Escherichia coliGertjan Veldhuis
Department of Biochemistry and Biophysical Chemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
Biophys J 89:201-10. 2005..The dependency could be simulated assuming that >50% of the binding sites were occupied with a ligand that shows an affinity for EnzymeIImtl in the same range as mannitol...- Engineering of ion sensing by the cystathionine beta-synthase module of the ABC transporter OpuANik A B N Mahmood
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, and Zernike Institute for Advanced Materials and Centre for Synthetic Biology, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Biol Chem 284:14368-76. 2009.... - Quaternary structure of SecA in solution and bound to SecYEG probed at the single molecule levelIlja Kusters
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, and Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
Structure 19:430-9. 2011..A structural model for the dimeric assembly of SecA while bound to SecYEG is proposed based on the crystal structures of the Thermotoga maritima SecA-SecYEG and the Escherichia coli SecA dimer... - Identification of the dimer interface of the lactose transport protein from Streptococcus thermophilusEric R Geertsma
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
J Mol Biol 332:1165-74. 2003..The four regions of the protein located at the dimer interface are close to the extracellular ends of transmembrane segments V and VIII and the intracellular ends of transmembrane segments VI and VII... - The oligomeric state and stability of the mannitol transporter, EnzymeII(mtl), from Escherichia coli: a fluorescence correlation spectroscopy studyGertjan Veldhuis
Department of Biochemistry and Biophysical Chemistry, Groningen Biomolecular Science and Biotechnology Institute and Materials Science Centreplus, University of Groningen, The Netherlands
Protein Sci 15:1977-86. 2006.... - Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteinsDaniel M Linares
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Mol Biol 401:45-55. 2010..The generality and simplicity of our approach allow rapid improvements of protein production yields by directed evolution in a high-throughput way... - The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membraneElisa B Vervoort
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Mol Biol 346:733-43. 2005..These and other results suggest that the predicted loop between TM2 and TM3 may fold back into the membrane and form part of the translocation path... - The structural basis for peptide selection by the transport receptor OppARonnie P A Berntsson
Biochemistry Department, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
EMBO J 28:1332-40. 2009..lactis is auxotrophic for branched chain amino acids and favours proline-rich caseins as a source of amino acids. We propose a new mechanism for peptide selection based on amino-acid composition rather than sequence... - Ligand binding and crystal structures of the substrate-binding domain of the ABC transporter OpuAJustina C Wolters
Biochemistry Department, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, University of Groningen, Groningen, The Netherlands
PLoS ONE 5:e10361. 2010..In this study, the ligand binding characteristics of purified OpuA in a detergent-solubilized state and of its substrate-binding domain produced as soluble protein (OpuAC) was characterized... - Structural divergence of paralogous S components from ECF-type ABC transportersRonnie P A Berntsson
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Proc Natl Acad Sci U S A 109:13990-5. 2012..Solitary BioY (in the absence of the ECF module) is monomeric in detergent solution and binds D-biotin with a high affinity but does not transport the substrate across the membrane... - Probing receptor-translocator interactions in the oligopeptide ABC transporter by fluorescence correlation spectroscopyMark K Doeven
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
Biophys J 94:3956-65. 2008..ATP-hydrolysis would complete the peptide translocation and reset the transporter for another catalytic cycle. Implications in terms of a general transport mechanism for ABC importers and exporters are discussed... - Structural basis for the enhanced activity of cyclic antimicrobial peptides: the case of BPC194Jacek T Mika
Department of Biochemistry and Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands
Biochim Biophys Acta 1808:2197-205. 2011..The results provide a chemical rationale for enhanced activity in certain cyclic antimicrobial peptides and can be used as a guideline for design of novel antimicrobial peptides... - Lipid packing drives the segregation of transmembrane helices into disordered lipid domains in model membranesLars V Schäfer
Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
Proc Natl Acad Sci U S A 108:1343-8. 2011.... - Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesiclesMark K Doeven
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Biophys J 88:1134-42. 2005..In addition, we show that sucrose slows down the lateral mobility of fluorescent lipid analogs, possibly due to hydrogen-bonding with the lipid headgroups, leading to larger complexes with reduced mobility... - Dual-color fluorescence-burst analysis to probe protein efflux through the mechanosensitive channel MscLGeert van den Bogaart
Biochemistry Department, Groningen Biomolecular Science and Biotechnology Institute and Materials Science Centre, University of Groningen, Groningen, The Netherlands
Biophys J 92:1233-40. 2007..For MscL, reconstituted in large unilamellar vesicles, we show that insulin, bovine pancreas trypsin inhibitor, and other compounds smaller than 6.5 kDa can pass through MscL, whereas larger macromolecules cannot... - The response of Lactococcus lactis to membrane protein productionRavi K R Marreddy
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
PLoS ONE 6:e24060. 2011.... - Cystathionine β-synthase (CBS) domains 1 and 2 fulfill different roles in ionic strength sensing of the ATP-binding cassette (ABC) transporter OpuAAkira Karasawa
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, Netherlands Proteomics Centre and Zernike Institute for Advanced Materials, University of Groningen, 9747 AG Groningen, The Netherlands
J Biol Chem 286:37280-91. 2011.... - Specificity and selectivity determinants of peptide transport in Lactococcus lactis and other microorganismsMark K Doeven
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Mol Microbiol 57:640-9. 2005..Besides reviewing the current data on binding specificity and transport selectivity of peptide transporters in L. lactis, the possible implications for peptide utilization by other bacterial species are discussed... - Dual-color fluorescence-burst analysis to study pore formation and protein-protein interactionsGeert van den Bogaart
Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Methods 46:123-30. 2008..In addition, DCFBA can be used to quantitatively measure the binding of proteins to liposomes and to membrane proteins. In this paper, we provide an overview of the method and discuss the experimental details of DCFBA... - The binding specificity of OppA determines the selectivity of the oligopeptide ATP-binding cassette transporterMark K Doeven
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
J Biol Chem 279:32301-7. 2004.... - Efficient overproduction of membrane proteins in Lactococcus lactis requires the cell envelope stress sensor/regulator couple CesSRJoao P C Pinto
Groningen Biomolecular Sciences and Biotechnology Institute, Department of Molecular Genetics, University of Groningen, Groningen, The Netherlands
PLoS ONE 6:e21873. 2011.... - Domain complementation studies reveal residues critical for the activity of the mannitol permease from Escherichia coliErwin P P Vos
Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Biochim Biophys Acta 1788:581-6. 2009..The involvement of specific residue positions in the oligomeric functioning of a sugar-translocating EII protein has not been presented before... - Long unfolded linkers facilitate membrane protein import through the nuclear pore complexAnne C Meinema
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, Netherlands
Science 333:90-3. 2011..We propose an import mechanism for membrane proteins in which an unfolded linker slices through the NPC scaffold to enable binding between the transport factor and the FG domains in the center of the NPC... - Importance of a hydrophobic pocket for peptide binding in lactococcal OppARonnie P A Berntsson
Biochemistry Department, Groningen Biomolecular Sciences and Biotechnology Center, NPC and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Bacteriol 193:4254-6. 2011..Two crystal structures of OppA with different nonapeptides now indeed show binding in different registers... - Substrate specificity and ionic regulation of GlnPQ from Lactococcus lactis. An ATP-binding cassette transporter with four extracytoplasmic substrate-binding domainsGea K Schuurman-Wolters
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
J Biol Chem 280:23785-90. 2005..The regulation of GlnPQ conforms to an electrostatic switch in which protein domain(s) and low molecular weight electrolytes participate... - Effect of iTRAQ labeling on the relative abundance of peptide fragment ions produced by MALDI-MS/MSTejas Gandhi
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
J Proteome Res 11:4044-51. 2012..This information will be useful for the development of improved method-specific intensity-based protein identification algorithms... - Evaluation of pulsed-FRAP and conventional-FRAP for determination of protein mobility in prokaryotic cellsJacek T Mika
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, Netherlands Proteomics Centre and Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
PLoS ONE 6:e25664. 2011..In this study, we have evaluated two of these methods: pulsed-FRAP and "conventional"-FRAP... - The ATP/substrate stoichiometry of the ATP-binding cassette (ABC) transporter OpuAJason S Patzlaff
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Biol Chem 278:29546-51. 2003..ADP is an inhibitor, but accumulation of ADP was negligible in the assays with inside-out-oriented OpuA, and potential effects of the ATP/ADP ratio on the ATP/substrate stoichiometry determinations could be eliminated... - Protein mobility and diffusive barriers in Escherichia coli: consequences of osmotic stressGeert van den Bogaart
Biochemistry Department, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, The Netherlands
Mol Microbiol 64:858-71. 2007..Also, lowering the osmolality from 0.6 Osm back to 0.15 Osm restored the mobility of GFP. The implications of these findings in terms of heterogeneities and diffusive barriers inside the cell are discussed... - On the mechanism of pore formation by melittinGeert van den Bogaart
Department of Biochemistry, the Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, 9747AG The Netherlands
J Biol Chem 283:33854-7. 2008..The direct insertion of melittin leads to pore formation, whereas the parallel conformation is inactive and prevents other melittin molecules from inserting, hence preventing pore formation... - Biochemistry. A missing link in membrane protein evolutionBert Poolman
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands
Science 315:1229-31. 2007 - The structural basis of modularity in ECF-type ABC transportersGuus B Erkens
University of Groningen, Groningen Biomolecular Science and Biotechnology Institute, Groningen, The Netherlands
Nat Struct Mol Biol 18:755-60. 2011..Based on these findings, we propose a general transport mechanism for ECF transporters... - Apex peptide elution chain selection: a new strategy for selecting precursors in 2D-LC-MALDI-TOF/TOF experiments on complex biological samplesTejas Gandhi
Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
J Proteome Res 9:5922-8. 2010..Consequently, reduced sample depletion allowed further selection of lower signal-to-noise ratio precursor ions, leading to a larger number of identified unique peptides... - Lateral diffusion of membrane proteins: consequences of hydrophobic mismatch and lipid compositionSivaramakrishnan Ramadurai
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre and the Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh, Groningen, The Netherlands
Biophys J 99:1482-9. 2010..We conclude that the hydrophobic thickness of the bilayer is a major determinant of molecule diffusion in membranes, but protein-specific properties may lead to deviations from the Saffman-Delbrück model... - The activity of the lactose transporter from Streptococcus thermophilus is increased by phosphorylated IIA and the action of beta-galactosidaseEric R Geertsma
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
Biochemistry 44:15889-97. 2005..The proposed mode of regulation thus proceeds via a mechanism opposite to the inducer exclusion type of regulation in gram-negative bacteria, where transporters are inhibited by binding of the unphosphorylated form of IIA(Glc)... - Influence of hydrophobic mismatch and amino acid composition on the lateral diffusion of transmembrane peptidesSivaramakrishnan Ramadurai
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, and Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
Biophys J 99:1447-54. 2010..The specific lipid headgroup composition of the membrane has a much less pronounced impact on the diffusion of the peptides than does the hydrophobic thickness... - Amino acid accumulation limits the overexpression of proteins in Lactococcus lactisRavi K R Marreddy
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
PLoS ONE 5:e10317. 2010..In this study, we employed a proteomic approach to understand the difference in expression levels for various multidomain membrane proteins in L. lactis cells grown in complex and synthetic media... - Structure and Mode of Peptide Binding of Pheromone Receptor PrgZRonnie P A Berntsson
From the Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, and Zernike Institute for Advanced Materials, University of Groningen, 9747 AG Groningen, The Netherlands and
J Biol Chem 287:37165-70. 2012..Comparison of PrgZ with homologous oligopeptide-binding proteins (AppA and OppA) explains the high specificity of PrgZ for hydrophobic heptapeptides versus the promiscuity of peptide binding in the homologous proteins... - Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolutionJan Peter Birkner
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, 9747 AG Groningen, The Netherlands
Proc Natl Acad Sci U S A 109:12944-9. 2012..Such a minimal change in the pore environment seems ideal for a fast and energy-efficient response to changes in the membrane tension... - Functional characterization of amphipathic α-helix in the osmoregulatory ABC transporter OpuANadia Gul
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, Netherlands Proteomics Centre and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Biochemistry 51:5142-52. 2012..We show that the amphipathic α-helix of OpuA is necessary for high activity of OpuA but is not critical for the biogenesis of the protein or the ionic regulation of transport... - Selenomethionine incorporation in proteins expressed in Lactococcus lactisRonnie P A Berntsson
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Protein Sci 18:1121-7. 2009..lactis for X-ray crystallography purposes. The crystal structure of ligand-free OppA was determined at 2.4 A resolution by a semiautomatic approach using selenium single-wavelength anomalous diffraction phasing... - Quality control of overexpressed membrane proteinsEric R Geertsma
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
Proc Natl Acad Sci U S A 105:5722-7. 2008..The method allows for rapid screening and, importantly, pinpointing the most likely bottlenecks in protein production... - Functional interactions between the subunits of the lactose transporter from Streptococcus thermophilusEric R Geertsma
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
J Mol Biol 350:102-11. 2005..In addition, analysis of in tandem fused LacS subunits containing one regulatory LacS-IIA domain showed that regulation is primarily an intramolecular event... - A karyopherin acts in localized protein synthesisLiesbeth M Veenhoff
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre and Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
Cell Cycle 9:1281-5. 2010..In this report, we reflect on the possible mechanisms by which Kap104p links these events and hypothesize on a possible function of the localized protein synthesis... - Ion specificity and ionic strength dependence of the osmoregulatory ABC transporter OpuAN A B Nik Mahmood
Membrane Enzymology Group, Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute and Materials Science Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Biol Chem 281:29830-9. 2006..Our data lend new support for the contention that the CBS module in OpuA constitutes the ionic strength sensor whose activity is modulated by the C-terminal anionic tail... - Lactococcus lactis uses MscL as its principal mechanosensitive channelJoost H A Folgering
Department of Biochemistry, Groninger Biomolecular Sciences and Biotechnology Institute and Material Science Centreplus, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
J Biol Chem 280:8784-92. 2005..The data strongly suggest that L. lactis uses MscL as the main mechanosensitive solute release system to protect the cells under conditions of osmotic downshift... - Functional analysis of detergent-solubilized and membrane-reconstituted ATP-binding cassette transportersBert Poolman
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Centre(plus, University of Groningen, The Netherlands
Methods Enzymol 400:429-59. 2005..Also, we present simple methods, based on substrate binding or translocation, to follow the activity of the protein complexes in detergent-solubilized and/or membrane-reconstituted state(s)... - Production of membrane proteins in Escherichia coli and Lactococcus lactisEric R Geertsma
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, University of Groningen, The Netherlands
Methods Mol Biol 601:17-38. 2010..Here, we detail methodology to rapidly establish and optimize (membrane) protein expression in Escherichia coli and Lactococcus lactis... - A method for site-specific labeling of multiple protein thiolsJohanna M Kuiper
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, 9747 AG Groningen, The Netherlands
Protein Sci 18:1033-41. 2009..The average labeling efficiency was approximately 80% for the single thiol and 65-90% for the dithiol site... - Membrane reconstitution of ABC transporters and assays of translocator functionEric R Geertsma
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Nat Protoc 3:256-66. 2008..The full protocol can be completed within 4-5 d... - A sensor for intracellular ionic strengthEsther Biemans-Oldehinkel
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Proc Natl Acad Sci U S A 103:10624-9. 2006..We present a model for the gating of membrane transport by ionic strength and propose a new role for CBS domains... - On the decrease in lateral mobility of phospholipids by sugarsGeert van den Bogaart
Biochemistry Department, Ultrafast Laser and Spectroscopy Laboratory, Groningen Biomolecular Science and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Biophys J 92:1598-605. 2007..Using coarse-grained molecular dynamics, we show that this increase in interactions can lead to a relatively large decrease in lateral phospholipid mobility... - Lipid-mediated light activation of a mechanosensitive channel of large conductanceJoost H A Folgering
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Center, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Langmuir 20:6985-7. 2004..Light-induced isomerization of the azobenzene moiety of 4-Azo-5P from trans to cis was used to activate MscL... - Two homologous oligopeptide binding protein genes (oppA) in Lactococcus lactis opp2 [corrected]Yolanda Sanz
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, NL 9751 NN, Haren, The Netherlands
Int J Food Microbiol 97:9-15. 2004..The differences in the ability to utilize this pentapeptide were not due to differences in expression levels but most likely reflect a different catalytic efficiency in oligopeptide utilization when AppA is used as ligand receptor... - Quaternary structure and function of transport proteinsLiesbeth M Veenhoff
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4 9747 AG Groningen, The Netherlands
Trends Biochem Sci 27:242-9. 2002..This review evaluates knowledge of the functions that are associated with the oligomeric organization of secondary transport proteins, which are a major class of solute-translocation systems in all living species... - Engineering covalent oligomers of the mechanosensitive channel of large conductance from Escherichia coli with native conductance and gating characteristicsJoost H A Folgering
Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
Protein Sci 14:2947-54. 2005..The in vivo data strongly suggest that pentameric MscL represents the functional state of the channel... - Design and synthesis of ATP-based nucleotide analogues and profiling of nucleotide-binding proteinsJustina C Wolters
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Bioconjug Chem 22:1345-53. 2011..p-BABA-ATP and p-BAMBA-ATP both bind to ATP-binding cassette (ABC) proteins with at least 10-fold higher affinity than ATP. Several ABC transporters could be enriched using p-BABA-ATP or p-BAMBA-ATP... - Proteomics of Saccharomyces cerevisiae OrganellesElena Wiederhold
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands Proteomics Centre and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Mol Cell Proteomics 9:431-45. 2010.... - Purification and functional reconstitution of the bacterial protein translocation pore, the SecYEG complexIlja Kusters
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
Methods Mol Biol 619:131-43. 2010..In addition, fluorescence based in vitro assays are described that allow monitoring translocation activity discontinuously and in real time... - On the role of the two extracytoplasmic substrate-binding domains in the ABC transporter OpuAEsther Biemans-Oldehinkel
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
EMBO J 22:5983-93. 2003..These new insights on the functional role of the extracytoplasmic SBDs are discussed in the light of the current knowledge of substrate-binding-protein-dependent ABC transporters... - A structural classification of substrate-binding proteinsRonnie P A Berntsson
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
FEBS Lett 584:2606-17. 2010.... - High-throughput cloning and expression in recalcitrant bacteriaEric R Geertsma
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
Nat Methods 4:705-7. 2007..We demonstrated VBEx proof of principle for Lactococcus lactis, but the method can be adapted to all organisms for which plasmids are available... - ABC transporter architecture and regulatory roles of accessory domainsEsther Biemans-Oldehinkel
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
FEBS Lett 580:1023-35. 2006..The regulatory domains add unique functions to the transporters allowing the systems to act as channel conductance regulators, osmosensors/regulators, and assemble into macromolecular complexes with specific properties... - Spatial organization of bacteriorhodopsin in model membranes. Light-induced mobility changesNicoletta Kahya
Ultrafast Laser and Spectroscopy Laboratory, Optical Sciences, Materials Science Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Biol Chem 277:39304-11. 2002..These observations are fully supported by freeze-fracture electron microscopy, and the size of the clusters during photoactivation was estimated to consist of two or three trimers... - Oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugationEsther H M L Heuberger
Department of Biochemistry Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
J Mol Biol 317:591-600. 2002..Our data underscore the fact that the oligomeric state of many membrane transport proteins is dimeric... - Mapping of the dimer interface of the Escherichia coli mannitol permease by cysteine cross-linkingBart A van Montfort
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Biol Chem 277:14717-23. 2002..In combination with the recently obtained projection structure of the IIC domain in two-dimensional crystals, a first proposal is made for alpha-helix packing in the mannitol permease... - The lipid dependence of melittin action investigated by dual-color fluorescence burst analysisGeert van den Bogaart
Biochemistry Department, Groningen Biomolecular Science and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
Biophys J 93:154-63. 2007..Experiments with liposomes prepared from Escherichia coli lipid extracts and intact cells of Lactococcus lactis indicate that both mechanisms are physiologically relevant... - Proteomic analysis of secreted membrane vesicles of archaeal Sulfolobus species reveals the presence of endosome sorting complex componentsAlbert F Ellen
Department of Molecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
Extremophiles 13:67-79. 2009..These data suggest that the membrane vesicles in Sulfolobus sp. emerge from a specific budding process with similarity to the endosomal sorting pathway... - ABC transporters: one, two or four extracytoplasmic substrate-binding sites?Tiemen van der Heide
Department of Biotehnology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
EMBO Rep 3:938-43. 2002..We also discuss the implications of multiple substrate-binding sites in close proximity to the translocator in terms of broadened substrate specificity and possible cooperative interactions between SBPs and the translocator... - Combined in-gel tryptic digestion and CNBr cleavage for the generation of peptide maps of an integral membrane protein with MALDI-TOF mass spectrometryBart A van Montfort
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
Biochim Biophys Acta 1555:111-5. 2002..The fragments formed are predominantly below m/z 2500, which allows accurate mass measurement... - Probing lipid mobility of raft-exhibiting model membranes by fluorescence correlation spectroscopyNicoletta Kahya
Experimental Biophysics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
J Biol Chem 278:28109-15. 2003..Moreover, FCS data from ternary and binary mixtures have been used to build a ternary phase diagram, which shows areas of phase coexistence, transition points, and, importantly, how lipid dynamics varies between and within phase regions... - Specificity of the second binding protein of the peptide ABC-transporter (Dpp) of Lactococcus lactis IL1403Yolanda Sanz
Instituto de Agroqui mica y Tecnologi a de Alimentos C S I C, Apartado 73, 46100, Valencia, Burjasot, Spain
FEMS Microbiol Lett 227:33-8. 2003..Remarkably, the features (ii), (iii) and (iv) differ from those previously reported for DppA-His(6), suggesting that DppP-His(6) is a more versatile peptide-binding protein that could have additional functions... - The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanismCatherine M Burgess
Alimentary Pharmabiotic Centre, Department of Microbiology and Biosciences Institute, National University of Ireland Cork, Western Road, Cork, Ireland
J Bacteriol 188:2752-60. 2006..The presented transport data are consistent with a uniport mechanism for riboflavin translocation and provide the first detailed molecular and functional analysis of a bacterial protein involved in riboflavin transport... - Electrochemical structure of the crowded cytoplasmJan J Spitzer
IPM Emulsion Polymer Research, 6643 Lyndonville Drive, Charlotte, NC 28277-4616, USA
Trends Biochem Sci 30:536-41. 2005..The electrochemical gradients are brought about by cellular biochemical reactions and by extracellular stimuli. The cellular metabolism is thus vectorial not only across the membrane but also throughout the cytoplasm... - In vitro functional characterization of BtuCD-F, the Escherichia coli ABC transporter for vitamin B12 uptakeElizabeth L Borths
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
Biochemistry 44:16301-9. 2005....