Geert J P L Kops

Summary

Affiliation: University Medical Center Utrecht
Country: The Netherlands

Publications

  1. pmc Chemical genetic inhibition of Mps1 in stable human cell lines reveals novel aspects of Mps1 function in mitosis
    Tale Sliedrecht
    Department of Physiological Chemistry, Cancer Genomics Centre, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 5:e10251. 2010
  2. pmc Release of Mps1 from kinetochores is crucial for timely anaphase onset
    Nannette Jelluma
    Department of Physiological Chemistry and Cancer Genomics Centre, UMC Utrecht, 3584 CG, Utrecht, Netherlands
    J Cell Biol 191:281-90. 2010
  3. pmc Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis
    Adrian T Saurin
    Molecular Cancer Research and Cancer Genomics Centre, University Medical Center Utrecht, Utrecht 3584 CG, The Netherlands
    Nat Commun 2:316. 2011
  4. pmc A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B
    Wilco Nijenhuis
    Department of Molecular Cancer Research, University Medical Center Utrecht, 3584 CG Utrecht, Netherlands
    J Cell Biol 201:217-31. 2013
  5. doi request reprint Cell division: SACing the anaphase problem
    Geert J P L Kops
    Molecular Cancer Research, Center for Molecular Medicine, and Cancer Genomics Netherlands, University Medical Center Utrecht, 3584 CG, Utrecht, The Netherlands Electronic address
    Curr Biol 24:R224-6. 2014
  6. doi request reprint Connecting up and clearing out: how kinetochore attachment silences the spindle assembly checkpoint
    Geert J P L Kops
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The Netherlands
    Chromosoma 121:509-25. 2012
  7. pmc APC16 is a conserved subunit of the anaphase-promoting complex/cyclosome
    Geert J P L Kops
    Department of Physiological Chemistry and Cancer Genomics Centre, UMC Utrecht, Utrecht, The Netherlands
    J Cell Sci 123:1623-33. 2010
  8. pmc Finding the middle ground: how kinetochores power chromosome congression
    Geert J P L Kops
    Department of Physiological Chemistry and Cancer Genomics Centre, University Medical Center Utrecht, Stratenum 3 217, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Cell Mol Life Sci 67:2145-61. 2010
  9. doi request reprint Dividing the goods: co-ordination of chromosome biorientation and mitotic checkpoint signalling by mitotic kinases
    Geert J P L Kops
    Department of Physiological Chemistry and Cancer Genomics Centre, UMC Utrecht, Utrecht, The Netherlands
    Biochem Soc Trans 37:971-5. 2009
  10. ncbi request reprint The kinetochore and spindle checkpoint in mammals
    Geert J P L Kops
    Department of Physiological Chemistry, UMC Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Front Biosci 13:3606-20. 2008

Collaborators

Detail Information

Publications31

  1. pmc Chemical genetic inhibition of Mps1 in stable human cell lines reveals novel aspects of Mps1 function in mitosis
    Tale Sliedrecht
    Department of Physiological Chemistry, Cancer Genomics Centre, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 5:e10251. 2010
    ..Proper functioning of the attachment and checkpoint processes is thus important to prevent chromosomal instability. Both processes rely on the mitotic kinase Mps1...
  2. pmc Release of Mps1 from kinetochores is crucial for timely anaphase onset
    Nannette Jelluma
    Department of Physiological Chemistry and Cancer Genomics Centre, UMC Utrecht, 3584 CG, Utrecht, Netherlands
    J Cell Biol 191:281-90. 2010
    ..We propose that release of Mps1 from kinetochores is essential for mitotic checkpoint silencing and a fast metaphase-to-anaphase transition...
  3. pmc Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis
    Adrian T Saurin
    Molecular Cancer Research and Cancer Genomics Centre, University Medical Center Utrecht, Utrecht 3584 CG, The Netherlands
    Nat Commun 2:316. 2011
    ..These data demonstrate a direct role for Aurora B in initiating the mitotic checkpoint rapidly at the onset of mitosis...
  4. pmc A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B
    Wilco Nijenhuis
    Department of Molecular Cancer Research, University Medical Center Utrecht, 3584 CG Utrecht, Netherlands
    J Cell Biol 201:217-31. 2013
    ..These data are consistent with a model in which Aurora B activity relieves a TPR-dependent inhibitory constraint on MPS1 localization...
  5. doi request reprint Cell division: SACing the anaphase problem
    Geert J P L Kops
    Molecular Cancer Research, Center for Molecular Medicine, and Cancer Genomics Netherlands, University Medical Center Utrecht, 3584 CG, Utrecht, The Netherlands Electronic address
    Curr Biol 24:R224-6. 2014
    ..Yet the very defects that trigger this checkpoint are inescapable consequences of sister chromatid segregation in anaphase. Three new studies provide clues to how cells cope with this problem. ..
  6. doi request reprint Connecting up and clearing out: how kinetochore attachment silences the spindle assembly checkpoint
    Geert J P L Kops
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The Netherlands
    Chromosoma 121:509-25. 2012
    ..Future challenges in this area are highlighted towards the goal of building a comprehensive molecular model of this process...
  7. pmc APC16 is a conserved subunit of the anaphase-promoting complex/cyclosome
    Geert J P L Kops
    Department of Physiological Chemistry and Cancer Genomics Centre, UMC Utrecht, Utrecht, The Netherlands
    J Cell Sci 123:1623-33. 2010
    ..rerio gene zgc:110659 are functional equivalents of human APC16. Our findings show that APC/C is composed of previously undescribed subunits, and raise the question of why metazoan APC/C is molecularly different from unicellular APC/C...
  8. pmc Finding the middle ground: how kinetochores power chromosome congression
    Geert J P L Kops
    Department of Physiological Chemistry and Cancer Genomics Centre, University Medical Center Utrecht, Stratenum 3 217, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Cell Mol Life Sci 67:2145-61. 2010
    ..Here we review the current knowledge and concepts on the processes that underlie chromosome congression, including initial attachment to spindle microtubules, biorientation, and movements, from the perspective of the kinetochore...
  9. doi request reprint Dividing the goods: co-ordination of chromosome biorientation and mitotic checkpoint signalling by mitotic kinases
    Geert J P L Kops
    Department of Physiological Chemistry and Cancer Genomics Centre, UMC Utrecht, Utrecht, The Netherlands
    Biochem Soc Trans 37:971-5. 2009
    ..A group of unrelated kinases controls various aspects of both processes. The present short review outlines our current understanding of the roles of these kinases in maintaining chromosomal stability...
  10. ncbi request reprint The kinetochore and spindle checkpoint in mammals
    Geert J P L Kops
    Department of Physiological Chemistry, UMC Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Front Biosci 13:3606-20. 2008
    ..This review focuses on molecular aspects of mitotic checkpoint signaling in mammals, including sensing improper attachments and transducing this information to the cell-cycle machinery...
  11. doi request reprint Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment
    Nannette Jelluma
    Department of Medical Oncology, Laboratory of Experimental Oncology, UMC Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Cell 132:233-46. 2008
    ..Mps1 thus coordinates attachment error correction and checkpoint signaling, two crucial responses to unproductive chromosome attachments...
  12. pmc Mps1 promotes rapid centromere accumulation of Aurora B
    Maike S van der Waal
    Department of Medical Oncology, University Medical Center Utrecht, Universiteitsweg 100, STR 2 129, 3584 CG Utrecht, The Netherlands
    EMBO Rep 13:847-54. 2012
    ....
  13. pmc Molecular causes for BUBR1 dysfunction in the human cancer predisposition syndrome mosaic variegated aneuploidy
    Saskia J E Suijkerbuijk
    Department of Physiological Chemistry and Cancer Genomics Centre, University Medical Center Utrecht, Utrecht, The Netherlands
    Cancer Res 70:4891-900. 2010
    ..Our findings provide a molecular explanation for chromosomal instability in patients with biallelic genetic mutations in BUBR1...
  14. pmc Chromosomal instability by inefficient Mps1 auto-activation due to a weakened mitotic checkpoint and lagging chromosomes
    Nannette Jelluma
    Department of Physiological Chemistry and Cancer Genomics Centre, UMC Utrecht, Utrecht, The Netherlands
    PLoS ONE 3:e2415. 2008
    ..Two of the causes of CIN are weakened mitotic checkpoint signaling and persistent merotelic attachments that result in lagging chromosomes during anaphase...
  15. ncbi request reprint Forkhead transcription factor FOXO3a protects quiescent cells from oxidative stress
    Geert J P L Kops
    Department of Physiological Chemistry, University Medical Center Utrecht and Center for Biomedical Genetics, 3584 CG Utrecht, The Netherlands
    Nature 419:316-21. 2002
    ..The model of Forkhead involvement in regulating longevity stems from genetic analysis in Caenorhabditis elegans, and we conclude that this model also extends to mammalian systems...
  16. pmc Elevating the frequency of chromosome mis-segregation as a strategy to kill tumor cells
    Aniek Janssen
    Department of Medical Oncology, Cancer Genomics Center, UMC Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 106:19108-13. 2009
    ..Thus, targeting the mitotic checkpoint and chromosome alignment simultaneously may selectively kill tumor cells by enhancing chromosome mis-segregations...
  17. doi request reprint Chromosome segregation errors as a cause of DNA damage and structural chromosome aberrations
    Aniek Janssen
    Department of Medical Oncology and Cancer Genomics Center, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, Netherlands
    Science 333:1895-8. 2011
    ..Our data show that segregation errors can cause translocations and provide insights into the role of whole-chromosome instability in tumorigenesis...
  18. doi request reprint Preventing aneuploidy: the contribution of mitotic checkpoint proteins
    Saskia J E Suijkerbuijk
    Department of Physiological Chemistry, UMC Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Biochim Biophys Acta 1786:24-31. 2008
    ..We propose that both checkpoint and non-checkpoint functions of these proteins contribute to the wide array of oncogenic phenotypes seen upon their misregulation...
  19. pmc Centromere binding and a conserved role in chromosome stability for SUMO-dependent ubiquitin ligases
    Loes A L van de Pasch
    Molecular Cancer Research, University Medical Centre Utrecht, Universiteitsweg 100, Utrecht, The Netherlands
    PLoS ONE 8:e65628. 2013
    ..The study shows that STUbLs have a conserved role in maintenance of chromosome stability and links SUMO-dependent ubiquitination to a centromere-specific function during mitosis...
  20. doi request reprint Integration of kinase and phosphatase activities by BUBR1 ensures formation of stable kinetochore-microtubule attachments
    Saskia J E Suijkerbuijk
    Molecular Cancer Research and Cancer Genomics Centre, UMC Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Dev Cell 23:745-55. 2012
    ..We propose that PLK1 and BUBR1 cooperate to stabilize kinetochore-microtubule interactions by regulating PP2A-B56α-mediated dephosphorylation of Aurora B substrates at the kinetochore-microtubule interface...
  21. doi request reprint The vertebrate mitotic checkpoint protein BUBR1 is an unusual pseudokinase
    Saskia J E Suijkerbuijk
    Molecular Cancer Research and Cancer Genomics Centre, Department of Medical Oncology, UMC Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Dev Cell 22:1321-9. 2012
    ..We propose that parallel evolution of BUBR1 orthologs rendered its kinase function dispensable in vertebrates, producing an unusual, triad-containing pseudokinase...
  22. doi request reprint Universal quantitative kinase assay based on diagonal SCX chromatography and stable isotope dimethyl labeling provides high-definition kinase consensus motifs for PKA and human Mps1
    Marco L Hennrich
    Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584CH, Utrecht, The Netherlands
    J Proteome Res 12:2214-24. 2013
    ..Our generic method, which makes use of proteolytic cell lysates as a source for peptide-substrate libraries, can be implemented for any kinase present in the kinome...
  23. pmc A phospho/methyl switch at histone H3 regulates TFIID association with mitotic chromosomes
    Radhika A Varier
    Department of Physiological Chemistry and Netherlands Proteomics Center, University Medical Centre Utrecht, Utrecht, The Netherlands
    EMBO J 29:3967-78. 2010
    ..Based on our observations, we propose that a histone H3 phospho-methyl switch regulates TFIID-mediated transcription during mitotic progression of the cell cycle...
  24. pmc Control of cell cycle exit and entry by protein kinase B-regulated forkhead transcription factors
    Geert J P L Kops
    Department of Physiological Chemistry, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands
    Mol Cell Biol 22:2025-36. 2002
    ..We therefore propose that Forkhead inactivation by PKB signaling in quiescent cells is a crucial step in cell cycle reentry and contributes to the processes of transformation and regeneration...
  25. doi request reprint Evolution and function of the mitotic checkpoint
    Mathijs Vleugel
    Department of Medical Oncology, Department of Molecular Cancer Research and Cancer Genomics Centre, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands
    Dev Cell 23:239-50. 2012
    ....
  26. ncbi request reprint On the road to cancer: aneuploidy and the mitotic checkpoint
    Geert J P L Kops
    Laboratory of Experimental Oncology, Department of Medical Oncology, University Medical Center, Utrecht, 3584 CG, The Netherlands
    Nat Rev Cancer 5:773-85. 2005
    ..Defects in the mitotic checkpoint generate aneuploidy and might facilitate tumorigenesis, but more severe disabling of checkpoint signalling is a possible anticancer strategy...
  27. pmc Centromere-associated protein-E is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single chromosome loss
    Beth A A Weaver
    Ludwig Institute for Cancer Research, 3080 CMM East, 9500 Gilman Drive, La Jolla, CA 92093 0670, USA
    J Cell Biol 162:551-63. 2003
    ..Thus, CENP-E is required for enhancing recruitment of its binding partner BubR1 to each unattached kinetochore and for stimulating BubR1 kinase activity, implicating it as an essential amplifier of a basal mitotic checkpoint signal...
  28. pmc ZW10 links mitotic checkpoint signaling to the structural kinetochore
    Geert J P L Kops
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    J Cell Biol 169:49-60. 2005
    ..Thus, ZW10 functions as a linker between the core structural elements of the outer kinetochore and components that catalyze generation of the mitotic checkpoint-derived "stop anaphase" inhibitor...
  29. pmc Lethality to human cancer cells through massive chromosome loss by inhibition of the mitotic checkpoint
    Geert J P L Kops
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093 0670, USA
    Proc Natl Acad Sci U S A 101:8699-704. 2004
    ..Thus, suppression of mitotic checkpoint signaling is invariably lethal as the consequence of massive chromosome loss, findings that have implications for inhibiting proliferation of tumor cells...
  30. ncbi request reprint The forkhead transcription factor FoxO regulates transcription of p27Kip1 and Bim in response to IL-2
    Marie Stahl
    Department of Molecular Biology H8, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Immunol 168:5024-31. 2002
    ..Thus, we propose that inactivation of FoxO transcription factors by IL-2 plays a critical role in T cell proliferation and survival...
  31. pmc Cell cycle inhibition by FoxO forkhead transcription factors involves downregulation of cyclin D
    Marc Schmidt
    Division of Molecular Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Mol Cell Biol 22:7842-52. 2002
    ..Ectopic expression of cyclin D1 can partially overcome FoxO factor-induced cell cycle arrest, demonstrating that downregulation of D-type cyclins represents a physiologically relevant mechanism of FoxO-induced cell cycle inhibition...