Genomes and Genes


J L Kluiver


Affiliation: University Medical Center Groningen
Country: The Netherlands


  1. Kluiver J, Niu F, Yuan Y, Kok K, van den Berg A, Dzikiewicz Krawczyk A. NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma. Methods Mol Biol. 2019;1956:269-282 pubmed publisher
    ..Second, we describe the procedure for next-generation sequencing library preparation. Third, we provide a workflow for data analysis. ..
  2. Kluiver J, Gibcus J, Hettinga C, Adema A, Richter M, Halsema N, et al. Rapid generation of microRNA sponges for microRNA inhibition. PLoS ONE. 2012;7:e29275 pubmed publisher
    ..In conclusion, we describe and validate a method to rapidly generate miRNA sponges for miRNA loss-of-function studies. ..
  3. Kluiver J, Slezak Prochazka I, Smigielska Czepiel K, Halsema N, Kroesen B, van den Berg A. Generation of miRNA sponge constructs. Methods. 2012;58:113-7 pubmed publisher
    ..In summary, our approach allows rapid generation of single or combination miRNA sponges that can be used for long-term miRNA loss-of-function studies. ..
  4. Winkle M, van den Berg A, Tayari M, Sietzema J, Terpstra M, Kortman G, et al. Long noncoding RNAs as a novel component of the Myc transcriptional network. FASEB J. 2015;29:2338-46 pubmed publisher
    ..This study is the first to show that lncRNAs are an important factor within the Myc-regulated transcriptional program and indicates a marked difference between Myc-repressed lncRNAs and mRNAs. ..
  5. Tayari M, Winkle M, Kortman G, Sietzema J, de Jong D, Terpstra M, et al. Long Noncoding RNA Expression Profiling in Normal B-Cell Subsets and Hodgkin Lymphoma Reveals Hodgkin and Reed-Sternberg Cell-Specific Long Noncoding RNAs. Am J Pathol. 2016;186:2462-72 pubmed publisher
    ..Three lncRNAs showed a tumor cell-specific expression pattern in HL tissues and might therefore be of value as a biomarker. ..
  6. Li J, Kluiver J, Osinga J, Westers H, van Werkhoven M, Seelen M, et al. Functional Studies on Primary Tubular Epithelial Cells Indicate a Tumor Suppressor Role of SETD2 in Clear Cell Renal Cell Carcinoma. Neoplasia. 2016;18:339-46 pubmed publisher
    ..Our results indicate that SETD2 inactivation enables PTECs to bypass the senescence barrier, facilitating a malignant transformation toward ccRCC. ..