Research Topics
Genomes and Genes | Jan A K W KielSummaryAffiliation: University of Groningen Country: The Netherlands Publications
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Publications
Degeneration of penicillin production in ethanol-limited chemostat cultivations of Penicillium chrysogenum: A systems biology approachRutger D Douma
Department of Biotechnology, Delft University of Technology, Kluyver Centre for Genomics of Industrial Fermentation, BC Delft, The Netherlands
BMC Syst Biol 5:132. 2011....
PEX genes in fungal genomes: common, rare or redundantJan A K W Kiel
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, PO Box 14, NL 9750 AA Haren, The Netherlands
Traffic 7:1291-303. 2006..These proteins belong to two groups of peroxins that we propose to designate the Pex11p and Pex23p families. This redundancy may complicate future studies on peroxisome biogenesis and proliferation in fungal species...- The 4th Hansenula polymorpha Worldwide Network Conference, Haren (The Netherlands), 3-5 September 2006Jan A K W Kiel
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, NL 9750 AA, Haren, The Netherlands
FEMS Yeast Res 7:167-71. 2007 - Overproduction of translation elongation factor 1-alpha (eEF1A) suppresses the peroxisome biogenesis defect in a Hansenula polymorpha pex3 mutant via translational read-throughJan A K W Kiel
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, The Netherlands
FEMS Yeast Res 7:1114-25. 2007..Subsequent co-overexpression of TEF2 in this strain fully restored its peroxisome biogenesis defect and resulted in the formation of major amounts of full-length Pex3p, presumably via translational read-through... 
Proteins involved in microbody biogenesis and degradation in Aspergillus nidulansJan A K W Kiel
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, NL 9750 AA Haren, The Netherlands
Fungal Genet Biol 46:S62-71. 2009..However, both processes have features that more closely resemble organelle formation/degradation in mammals rather than yeast. Thus, filamentous fungi like A. nidulans are ideal model systems for peroxisome homeostasis in man...- Matching the proteome to the genome: the microbody of penicillin-producing Penicillium chrysogenum cellsJan A K W Kiel
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, AA Haren, The Netherlands
Funct Integr Genomics 9:167-84. 2009..We show that this protein indeed localizes to P. chrysogenum microbodies... - Autophagy in unicellular eukaryotesJan A K W Kiel
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, 9750 Haren, The Netherlands
Philos Trans R Soc Lond B Biol Sci 365:819-30. 2010..Additionally, a detailed analysis will be presented on the state of knowledge on autophagy in non-yeast unicellular eukaryotes with emphasis on the role of this process in differentiation... - Penicillium chrysogenum Pex5p mediates differential sorting of PTS1 proteins to microbodies of the methylotrophic yeast Hansenula polymorphaJan A K W Kiel
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, P O Box 14, Haren NL 9750 AA, The Netherlands
Fungal Genet Biol 41:708-20. 2004..polymorpha counterpart. This was also suggested by the observation that Pc-Pex5p sorted a reporter protein fused to various functional PTS1 signals with different efficiencies... - Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorphaAdriana N Leão-Helder
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
FEBS Lett 577:491-5. 2004..In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete... - Novel genetic tools for Hansenula polymorphaRuchi Saraya
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, Kluyver Centre for Genomics of Industrial Fermentation, University of Groningen, Groningen, The Netherlands
FEMS Yeast Res 12:271-8. 2012..Finally, we show the implementation of Gateway technology and a single-step PCR strategy to create deletions in H. polymorpha... - Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl geneLoknath Gidijala
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB University of Groningen, Haren, The Netherlands
FEMS Yeast Res 7:1160-7. 2007..Mutation of the PTS1 of PCL (SKI-COOH) to SKL-COOH restored sorting of the fusion protein to peroxisomes only. Additionally, we demonstrate that peroxisomal PCL-SKL produced in H. polymorpha displays normal enzymatic activities... - Peroxisome fission in Hansenula polymorpha requires Mdv1 and Fis1, two proteins also involved in mitochondrial fissionShirisha Nagotu
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands
Traffic 9:1471-84. 2008..Also, in H. polymorpha mdv1 or fis1 cells, the number of Dnm1-GFP spots is strongly reduced. These spots still associate to organelles but are functionally inactive... - Hansenula polymorpha Swi1p and Snf2p are essential for methanol utilisationPaulina Ozimek
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands
FEMS Yeast Res 4:673-82. 2004..The level of Pex5p, the receptor involved in import of alcohol oxidase and dihydroxyacetone synthase into peroxisomes, was also reduced in both disruption strains compared to that in wild-type cells... - Atg8 is essential for macropexophagy in Hansenula polymorphaIryna Monastyrska
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB, University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands
Traffic 6:66-74. 2005..We hypothesize that H. polymorpha Atg8 facilitates sealing of the sequestering membranes during selective peroxisome degradation... - An engineered yeast efficiently secreting penicillinLoknath Gidijala
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Haren, The Netherlands
PLoS ONE 4:e8317. 2009..g. immunosuppressive and cytostatic agents), whose production involves NRPS's... - Peroxisomes are required for efficient penicillin biosynthesis in Penicillium chrysogenumWiebe H Meijer
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, P O Box 14, 9750 AA Haren, The Netherlands
Appl Environ Microbiol 76:5702-9. 2010..We observed that the organelle proliferation machinery requires the dynamin-like protein Dnm1... - De novo peroxisome biogenesis in Penicillium chrysogenum is not dependent on the Pex11 family members or Pex16Łukasz Opaliński
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, AG Groningen, The Netherlands
PLoS ONE 7:e35490. 2012..chrysogenum. Remarkably, distinct from earlier results with low penicillin producing laboratory strains, upregulation of peroxisome numbers in a high producing P. chrysogenum strain had no significant effect on penicillin production... - Overproduction of a single protein, Pc-Pex11p, results in 2-fold enhanced penicillin production by Penicillium chrysogenumJan A K W Kiel
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, PO Box 14, NL 9750 AA Haren, The Netherlands
Fungal Genet Biol 42:154-64. 2005..Our results suggest that the stimulating effect of enhanced organelle numbers may reflect an increase in the fluxes of penicillin and/or its precursors across the now much enlarged microbody membrane... - ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genesWiebe H Meijer
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
Autophagy 3:106-16. 2007..This suggests that in fungi an organism-specific form of selective autophagy may occur, for which specialized Atg proteins have evolved... - Autophagy deficiency promotes beta-lactam production in Penicillium chrysogenumMagdalena Bartoszewska
Molecular Cell Biology, University of Groningen, P O Box 14, 9750 AA Haren, The Netherlands
Appl Environ Microbiol 77:1413-22. 2011..This phenomenon is associated with an increase in the enzyme levels of the PEN biosynthetic pathway and enhanced production levels of this antibacterial compound... - Selective degradation of peroxisomes in yeastsAnna Rita Bellu
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB, University of Groningen, 9751 NN Haren, The Netherlands
Microsc Res Tech 61:161-70. 2003..This review highlights the main achievements of the last years... - A conserved alpha helical domain at the N-terminus of Pex14p is required for PTS1 and PTS2 protein import in Hansenula polymorphaBart de Vries
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, 9750 AA, Haren, The Netherlands
FEBS Lett 581:5627-34. 2007..An extensive mutational analysis of the first alpha-helix (aa 10-21) demonstrated that its secondary structure, as well as residues Phe20 and Leu21, are essential for PTS1 and PTS2 matrix protein import... - A conserved function for Inp2 in peroxisome inheritanceRuchi Saraya
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, AA Haren, The Netherlands
Biochim Biophys Acta 1803:617-22. 2010..Our data show that H. polymorpha Inp2 locates to peroxisomes, interacts with Myo2, and is essential for peroxisome inheritance... - The Hansenula polymorpha ATG25 gene encodes a novel coiled-coil protein that is required for macropexophagyIryna Monastyrska
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
Autophagy 1:92-100. 2005..polymorpha is only observed at nitrogen limitation conditions. This suggests that nonselective microautophagy is deregulated in H. polymorpha atg25 cells... - Hansenula polymorpha Vam7p is required for macropexophagyPatricia Stevens
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB, University of Groningen, P.O. Box 14, 9751 AA Haren, The Netherlands
FEMS Yeast Res 5:985-97. 2005..These morphological defects became first visible in cells that were in the mid-exponential growth phase of cultivation on methanol, and were correlated with accumulation of electron-dense extensions that were connected to mitochondria... - Obstruction of polyubiquitination affects PTS1 peroxisomal matrix protein importJan A K W Kiel
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB, University of Groningen, P.O. Box 14, NL-9750 AA Haren, The Netherlands
Biochim Biophys Acta 1745:176-86. 2005..We speculate that the degradation of Pex5p by the proteasome is important to remove receptor molecules that are stuck at a late stage of the Pex5p-mediated protein import pathway... - Removal of Pex3p is an important initial stage in selective peroxisome degradation in Hansenula polymorphaAnna Rita Bellu
Eukaryotic Microbiology, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, Biological Center, P. O. Box 14, 9750 AA Haren, The Netherlands
J Biol Chem 277:42875-80. 2002..Taken together, our data suggest that degradation of Pex3p is part of the initial degradation machinery of individual peroxisomes... - The Hansenula polymorpha PDD7 gene is essential for macropexophagy and microautophagyJanet A Komduur
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands
FEMS Yeast Res 3:27-34. 2003..Our data indicate that HpPdd7p is essential for two processes that degrade peroxisomes, namely the highly selective process of macropexophagy and microautophagy, which occurs in H. polymorpha upon nitrogen starvation... - Membrane curvature during peroxisome fission requires Pex11Łukasz Opaliński
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, Haren, The Netherlands
EMBO J 30:5-16. 2011..Finally, we demonstrate that mutations abolishing the membrane remodelling activity of the Pex11-Amph domain also hamper the function of full-length Pex11 in peroxisome fission in vivo... - Penicillium chrysogenum Pex14/17p--a novel component of the peroxisomal membrane that is important for penicillin productionLukasz Opaliński
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
FEBS J 277:3203-18. 2010..Possibly, this compensates for the fact that P. chrysogenum lacks an authenthic Pex17p. We also show that, in P. chrysogenum, Pex14/17p plays a role in making the penicillin biosynthesis process more efficient... - Preserving organelle vitality: peroxisomal quality control mechanisms in yeastEda Bener Aksam
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Haren, The Netherlands
FEMS Yeast Res 9:808-20. 2009..This review provides an overview of the major processes involved in quality control of yeast peroxisomes... - A peroxisomal lon protease and peroxisome degradation by autophagy play key roles in vitality of Hansenula polymorpha cellsEda Bener Aksam
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, Haren, The Netherlands
Autophagy 3:96-105. 2007.... - Hansenula polymorpha Tup1p is important for peroxisome degradationAdriana N Leão-Helder
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, P O Box 14, 9750 AA Haren, The Netherlands
FEMS Yeast Res 4:789-94. 2004..The initial DNA fragment that complemented H. polymorpha pdd2-4 contained a second gene, encoding H. polymorpha Vps4p. Deletion of the VPS4 gene did not affect selective peroxisome degradation... - Production of functionally active Penicillium chrysogenum isopenicillin N synthase in the yeast Hansenula polymorphaLoknath Gidijala
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, P O Box 14, 9750 AA Haren, The Netherlands
BMC Biotechnol 8:29. 2008..One of the fundamental challenges is to produce functionally active enzyme in H. polymorpha... - Peroxisome homeostasis in Hansenula polymorphaAdriana N Leão
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands
FEMS Yeast Res 4:131-9. 2003..Thus, it has become essential to study in detail the role of peroxisomal membrane proteins in the degradation process. This review highlights the main achievements of the last few years, with emphasis on H. polymorpha... - Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorphaAdriana Nívea Leao Helder
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren 9750 AA, The Netherlands
J Biol Chem 278:40749-56. 2003..We show that this mechanism is a general phenomenon in H. polymorpha in the case of cells that contain only a single peroxisome... - The Penicillium chrysogenum aclA gene encodes a broad-substrate-specificity acyl-coenzyme A ligase involved in activation of adipic acid, a side-chain precursor for cephem antibioticsMartijn J Koetsier
Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, 9747 AG Groningen, The Netherlands
Fungal Genet Biol 47:33-42. 2010..Identification and functional characterization of aclA may aid in developing future metabolic engineering strategies for improving the production of different cephalosporins... - Macropexophagy in Hansenula polymorpha: facts and viewsJan A K W Kiel
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands
FEBS Lett 549:1-6. 2003..Here, we highlight recent achievements in regulation of peroxisome turnover in yeast, in particular Hansenula polymorpha, with a focus on directions of future research... - Pexophagy in Hansenula polymorphaTim van Zutphen
Molecular Cell Biology, University of Groningen, The Netherlands
Methods Enzymol 451:197-215. 2008..polymorpha. Emphasis is placed on various structural (fluorescence microscopy, electron microscopy) and biochemical (specific enzyme activity measurements, Western blotting) approaches... - Hansenula polymorpha Pex3p is a peripheral component of the peroxisomal membraneGert Jan Haan
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB, University of Groningen, P. O. Box 14, 9750 AA Haren, The Netherlands
J Biol Chem 277:26609-17. 2002..Taken together, our data suggest that HpPex3p does not span the peroxisomal membrane but instead is tightly associated to the cytosolic face of the organelle where it may be present in focal protein clusters... - Characterization of the Hansenula polymorpha PUR7 gene and its use as selectable marker for targeted chromosomal integrationGert Jan Haan
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands
FEMS Yeast Res 2:17-24. 2002..The advantages of pHIPA4 and the new triple auxotrophic strain in relation to the use of H. polymorpha as a versatile cell factory or as a model organism for fundamental studies on the principles of peroxisome homeostasis is discussed... - Uptake of the beta-lactam precursor alpha-aminoadipic acid in Penicillium chrysogenum is mediated by the acidic and the general amino acid permeaseHein Trip
Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
Appl Environ Microbiol 70:4775-83. 2004..chrysogenum. The transport measurements demonstrate that both PcGap1 and PcDip5 contribute to the alpha-aminoadipic acid flux... - Peroxisome assembly in yeastMarten Veenhuis
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, NL 9750 AA Haren, The Netherlands
Microsc Res Tech 61:139-50. 2003..This review summarizes relevant recent data on this subject. In addition, the progress in the understanding of the principles of the peroxisomal matrix protein import machinery is discussed... - Old yellow enzyme confers resistance of Hansenula polymorpha towards allyl alcoholJanet A Komduur
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
Curr Genet 41:401-6. 2002..Our data suggest that the proteins encoded by these genes detoxify acrolein by converting it into less harmful components... - The role of macroautophagy in development of filamentous fungiMagdalena Bartoszewska
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
Antioxid Redox Signal 14:2271-87. 2011..This review also summarizes the state of knowledge on the role of autophagy in filamentous fungi during differentiation, such as pathogenic development, programmed cell death during heteroincompatibility, and spore formation... - Microautophagy and macropexophagy may occur simultaneously in Hansenula polymorphaIryna Monastryska
Laboratory of Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands
FEBS Lett 568:135-8. 2004..Our combined data strongly suggest that both processes occur simultaneously under these conditions. The implications of these findings on studies of autophagy and related transport pathways to the vacuole in yeast are discussed... - Pexophagy: the selective autophagy of peroxisomesWilliam A Dunn
Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, Florida, USA
Autophagy 1:75-83. 2005..Utilizing several genetic screens, we have identified a number of genes that are essential for pexophagy. In this review, we will summarize our current knowledge of the molecular events of pexophagy... - Ubiquitination of the peroxisomal targeting signal type 1 receptor, Pex5p, suggests the presence of a quality control mechanism during peroxisomal matrix protein importJan A K W Kiel
Abteilung für Zellbiochemie, Medizinische Fakultät der Ruhr Universität Bochum, Germany
J Biol Chem 280:1921-30. 2005..Our data suggest that during receptor recycling a portion of Pex5p becomes ubiquitinated and degraded by the proteasome. We propose that this process represents a conserved quality control mechanism in peroxisome biogenesis... - Pexophagy: autophagic degradation of peroxisomesYasuyoshi Sakai
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Kyoto 606 8502, Japan
Biochim Biophys Acta 1763:1767-75. 2006..Subsequently, the engulfed peroxisome cluster is degraded. This review discusses the current state of knowledge of pexophagy with emphasis on studies on methylotrophic yeast species...