D B Janssen

Summary

Affiliation: University of Groningen
Country: The Netherlands

Publications

  1. ncbi request reprint Steady-state and pre-steady-state kinetic analysis of halopropane conversion by a rhodococcus haloalkane dehalogenase
    Tjibbe Bosma
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biochemistry 42:8047-53. 2003
  2. ncbi request reprint Synthesis of enantiopure chloroalcohols by enzymatic kinetic resolution
    Robert M Haak
    Department of Organic and Molecular Inorganic Chemistry, Stratingh Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Org Biomol Chem 5:318-23. 2007
  3. doi request reprint Computationally designed libraries for rapid enzyme stabilization
    Hein J Wijma
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Protein Eng Des Sel 27:49-58. 2014
  4. pmc Transformation and biodegradation of 1,2,3-trichloropropane (TCP)
    Ghufrana Samin
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Environ Sci Pollut Res Int 19:3067-78. 2012
  5. pmc Biodegradation kinetics of 4-fluorocinnamic acid by a consortium of Arthrobacter and Ralstonia strains
    Syed A Hasan
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, The Netherlands
    Biodegradation 23:117-25. 2012
  6. pmc Degradation of 4-fluorophenol by Arthrobacter sp. strain IF1
    Maria Isabel M Ferreira
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, NL 9747 AG, Groningen, The Netherlands
    Appl Microbiol Biotechnol 78:709-17. 2008
  7. ncbi request reprint Enantioselective formation and ring-opening of epoxides catalysed by halohydrin dehalogenases
    D B Janssen
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, Groningen, The Netherlands
    Biochem Soc Trans 34:291-5. 2006
  8. ncbi request reprint Bacterial degradation of xenobiotic compounds: evolution and distribution of novel enzyme activities
    Dick B Janssen
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Environ Microbiol 7:1868-82. 2005
  9. ncbi request reprint Kinetic characterization and X-ray structure of a mutant of haloalkane dehalogenase with higher catalytic activity and modified substrate range
    J P Schanstra
    Laboratory of Biochemistry, BIOSON Research Institute, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Biochemistry 35:13186-95. 1996
  10. ncbi request reprint Repositioning the catalytic triad aspartic acid of haloalkane dehalogenase: effects on stability, kinetics, and structure
    G H Krooshof
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biochemistry 36:9571-80. 1997

Collaborators

  • M W Fraaije
  • L Tang
  • David Baker
  • Siewert J Marrink
  • Gerrit J Poelarends
  • J R Marchesi
  • E de Vries
  • E M Sipkema
  • M J Larkin
  • H Baldascini
  • J Damborsky
  • G van Hall
  • L A Kulakov
  • R Rink
  • J Koca
  • Maciej Torz
  • Y Nagata
  • Theo Sonke
  • Erik W van Hellemond
  • Bert van Loo
  • Wynand B L Alkema
  • René M de Jong
  • Syed A Hasan
  • Maria Isabel M Ferreira
  • Dominic P H M Heuts
  • Robert H H van den Heuvel
  • Mariel G Pikkemaat
  • Jaap Kingma
  • Bauke W Dijkstra
  • Esther M Gabor
  • Bernhard Hauer
  • Peter A Jekel
  • Maja Majeric Elenkov
  • J P Schanstra
  • Tjibbe Bosma
  • Hein J Wijma
  • Ghufrana Samin
  • Nanne M Kamerbeek
  • Lachlan S Campbell-Verduyn
  • Jolanda J Polderman-Tijmes
  • B W Dijkstra
  • Ben L Feringa
  • Ghannia Hasnaoui-Dijoux
  • Maja Majerić Elenkov
  • Hortense Mazon
  • Robert M Haak
  • Jianfeng Jin
  • J E van Hylckama Vlieg
  • Maria F Carvalho
  • Thomas R M Barends
  • Jeffrey H Lutje Spelberg
  • Marcel G Wubbolts
  • G H Krooshof
  • Kor H Kalk
  • K H Kalk
  • I J T Dinkla
  • Zbynek Prokop
  • R M de Jong
  • Robert J Floor
  • I J Dinkla
  • Piet Wietzes
  • N M Kamerbeek
  • J H Spelberg
  • H J Rozeboom
  • Muhammad I Arif
  • Martijn J Koetsier
  • Rudi A Dierckx
  • Wiktor Szymanski
  • Philip H Elsinga
  • Christiaan P Postema
  • W B Alkema
  • Hjalmar P Permentier
  • Albert J Heck
  • Auke Meetsma
  • Marianne van Dijk
  • Johannes G de Vries
  • Adriaan J Minnaard
  • Chiara Tarabiono
  • Paula M L Castro
  • Michael Arand
  • Gjalt Wybenga
  • Irina S Moreira
  • Christopher Williams
  • Nora Tahallah
  • Jan J W Tiesinga
  • Willem J H van Berkel
  • Albert J R Heck
  • Alessandra Villa
  • I S Ridder
  • Marta Monincová

Detail Information

Publications67

  1. ncbi request reprint Steady-state and pre-steady-state kinetic analysis of halopropane conversion by a rhodococcus haloalkane dehalogenase
    Tjibbe Bosma
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biochemistry 42:8047-53. 2003
    ..However, whereas in DhlA the rate of halide release represents the slowest step in the catalytic cycle, our results suggest that in DhaA the release of 3-bromo-1-propanol is the slowest step during 1,3-dibromopropane conversion...
  2. ncbi request reprint Synthesis of enantiopure chloroalcohols by enzymatic kinetic resolution
    Robert M Haak
    Department of Organic and Molecular Inorganic Chemistry, Stratingh Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Org Biomol Chem 5:318-23. 2007
    ..Yields were close to the theoretical maximum for all substrates employed. Furthermore, the applicability of this methodology on multigram scale has been established...
  3. doi request reprint Computationally designed libraries for rapid enzyme stabilization
    Hein J Wijma
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Protein Eng Des Sel 27:49-58. 2014
    ..The developed Framework for Rapid Enzyme Stabilization by Computational libraries (FRESCO) requires far less screening than conventional directed evolution. ..
  4. pmc Transformation and biodegradation of 1,2,3-trichloropropane (TCP)
    Ghufrana Samin
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Environ Sci Pollut Res Int 19:3067-78. 2012
    ..This paper presents an overview of methods for the remediation of TCP-contaminated water with an emphasis on the possibilities of biodegradation...
  5. pmc Biodegradation kinetics of 4-fluorocinnamic acid by a consortium of Arthrobacter and Ralstonia strains
    Syed A Hasan
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, The Netherlands
    Biodegradation 23:117-25. 2012
    ....
  6. pmc Degradation of 4-fluorophenol by Arthrobacter sp. strain IF1
    Maria Isabel M Ferreira
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, NL 9747 AG, Groningen, The Netherlands
    Appl Microbiol Biotechnol 78:709-17. 2008
    ..These results indicate that the biodegradation of 4-FP starts with a 4-FP monooxygenase reaction that yields benzoquinone, which is reduced to hydroquinone and further metabolized via the beta-ketoadipic acid pathway...
  7. ncbi request reprint Enantioselective formation and ring-opening of epoxides catalysed by halohydrin dehalogenases
    D B Janssen
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, Groningen, The Netherlands
    Biochem Soc Trans 34:291-5. 2006
    ....
  8. ncbi request reprint Bacterial degradation of xenobiotic compounds: evolution and distribution of novel enzyme activities
    Dick B Janssen
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Environ Microbiol 7:1868-82. 2005
    ....
  9. ncbi request reprint Kinetic characterization and X-ray structure of a mutant of haloalkane dehalogenase with higher catalytic activity and modified substrate range
    J P Schanstra
    Laboratory of Biochemistry, BIOSON Research Institute, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Biochemistry 35:13186-95. 1996
    ..It is proposed that Phe172 is involved in stabilization of the helix-loop-helix structure that covers the active site of the enzyme and creates a rigid hydrophobic cavity for small apolar halogenated alkanes...
  10. ncbi request reprint Repositioning the catalytic triad aspartic acid of haloalkane dehalogenase: effects on stability, kinetics, and structure
    G H Krooshof
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biochemistry 36:9571-80. 1997
    ....
  11. ncbi request reprint Kinetic analysis and X-ray structure of haloalkane dehalogenase with a modified halide-binding site
    G H Krooshof
    Laboratory of Biochemistry, BIOSON Research Institute, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Biochemistry 37:15013-23. 1998
    ..These structural changes indicate a weakened interaction between residue 175 and the halogen atom or halide ion in the active site and help to explain the kinetic changes induced by the Trp175Tyr mutation...
  12. ncbi request reprint Kinetics of halide release of haloalkane dehalogenase: evidence for a slow conformational change
    J P Schanstra
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Biochemistry 35:5624-32. 1996
    ..2H2O mainly affected the rate of the conformational change, which is in agreement with this step being rate-limiting and the overall stabilizing effect of 2H2O on the conformation of proteins...
  13. pmc Roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways
    G J Poelarends
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
    J Bacteriol 182:2191-9. 2000
    ..The data indicate that horizontal gene transfer and integrase-dependent gene acquisition were the key mechanisms for the evolution of catabolic pathways for the man-made chemicals 1, 3-dichloropropene and 1,2-dibromoethane...
  14. ncbi request reprint Specificity and kinetics of haloalkane dehalogenase
    J P Schanstra
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Biol Chem 271:14747-53. 1996
    ..The study provides a basis for the analysis of rate-determining steps in the hydrolysis of various environmentally important substrates...
  15. ncbi request reprint Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies
    W B Alkema
    Department of Biochemistry, BIOSON Research Institute, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Protein Eng 13:857-63. 2000
    ..The combined results of the structural and kinetic studies show the importance of alphaF146 in the beta-lactam binding site and provide leads for engineering mutants with improved synthetic properties...
  16. ncbi request reprint Highly enantioselective and regioselective biocatalytic azidolysis of aromatic epoxides
    J H Spelberg
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, and Department of Organic and Molecular Inorganic Chemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Org Lett 3:41-3. 2001
    ..In a large scale conversion, the decrease in yield and selectivity due to the uncatalyzed chemical side reaction could be overcome by slow addition of azide...
  17. pmc Degradation of 1,2-dibromoethane by Mycobacterium sp. strain GP1
    G J Poelarends
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
    J Bacteriol 181:2050-8. 1999
    ..rhodochrous NCIMB 13064, was fused to a fragment of a haloalcohol dehalogenase gene that was identical to the last 42 nucleotides of the hheB gene found in Corynebacterium sp. strain N-1074...
  18. ncbi request reprint Influence of mutations of Val226 on the catalytic rate of haloalkane dehalogenase
    J P Schanstra
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Protein Eng 10:53-61. 1997
    ..These studies indicate that wild-type haloalkane dehalogenase is optimized for 1,2-dichloroethane, although 1,2-dibromoethane is a better substrate...
  19. pmc Halohydrin dehalogenases are structurally and mechanistically related to short-chain dehydrogenases/reductases
    J E van Hylckama Vlieg
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, NL-9747 AG Groningen, The Netherlands
    J Bacteriol 183:5058-66. 2001
    ..The proposed mechanism is fundamentally different from that of the well-studied hydrolytic dehalogenases, since it does not involve a covalent enzyme-substrate intermediate...
  20. ncbi request reprint 4-Hydroxyacetophenone monooxygenase from Pseudomonas fluorescens ACB. A novel flavoprotein catalyzing Baeyer-Villiger oxidation of aromatic compounds
    N M Kamerbeek
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Eur J Biochem 268:2547-57. 2001
    ..From a kinetic analysis of the G490A mutant it is concluded that the observed sequence motif serves a structural function which is of importance for NADPH binding...
  21. pmc Effects of iron limitation on the degradation of toluene by Pseudomonas strains carrying the tol (pWWO) plasmid
    I J Dinkla
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
    Appl Environ Microbiol 67:3406-12. 2001
    ..Growth yields were reduced and substrate affinities decreased under iron-limited conditions, suggesting that iron availability can be an important parameter in the oxidative breakdown of hydrocarbons...
  22. pmc trans-3-Chloroacrylic acid dehalogenase from Pseudomonas pavonaceae 170 shares structural and mechanistic similarities with 4-oxalocrotonate tautomerase
    G J Poelarends
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
    J Bacteriol 183:4269-77. 2001
    ..Based on these findings, a novel dehalogenation mechanism is proposed for the CaaD-catalyzed reaction which does not involve the formation of a covalent enzyme-substrate intermediate...
  23. ncbi request reprint Effect of mass transfer limitations on the enzymatic kinetic resolution of epoxides in a two-liquid-phase system
    H Baldascini
    Department of Chemical Engineering, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Biotechnol Bioeng 73:44-54. 2001
    ..Resolution of a 39 g/L solution of racemic styrene oxide in octane was successfully carried out in an emulsion batch reactor to obtain (S)-styrene oxide in high enantiomeric excess (>95% e.e.) with a yield of 30%...
  24. ncbi request reprint Trichloroethene degradation in a two-step system by methylosinus trichosporium OB3b. Optimization of system performance: use of formate and methane
    E M Sipkema
    Department of Chemical Engineering, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biotechnol Bioeng 63:56-68. 1999
    ..The model provides a tool for the design of an effective and low-cost treatment system based on methane addition in the conversion reactor...
  25. ncbi request reprint Microbial dehalogenation
    D B Janssen
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, NL 9747 AG, Groningen, The Netherlands
    Curr Opin Biotechnol 12:254-8. 2001
    ..Sequence analysis and mutagenesis studies indicate that several dehalogenases are homologous to enzymes that carry out transformations on non-halogenated substrates...
  26. pmc Structure and mechanism of a bacterial haloalcohol dehalogenase: a new variation of the short-chain dehydrogenase/reductase fold without an NAD(P)H binding site
    R M de Jong
    Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, NL 9747 AG Groningen, The Netherlands
    EMBO J 22:4933-44. 2003
    ..Structure-based sequence analysis reveals that the various haloalcohol dehalogenases have likely originated from at least two different NAD-binding SDR precursors...
  27. pmc Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene
    J van der Ploeg
    Department of Biochemistry, Groningen Biotechnology Center, University of Groningen, The Netherlands
    J Bacteriol 173:7925-33. 1991
    ..5 and 61.0% similarity) with the two other haloacid dehalogenases sequenced to date but not with the haloalkane dehalogenase from X. autotrophicus GJ10...
  28. ncbi request reprint Simultaneous growth on citrate reduces the effects of iron limitation during toluene degradation in Pseudomonas
    I J T Dinkla
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Microb Ecol 45:97-107. 2003
    ..The results indicate that the availability of low concentrations of natural organic compounds, such as produced in the rhizosphere, may positively influence the degradative performance of hydrocarbon-degrading bacteria...
  29. pmc Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox
    Mariel G Pikkemaat
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands
    Nucleic Acids Res 30:E35-5. 2002
    ..The results show that the mutagenesis method presented here is an effective tool for accessing formerly unexplorable sequence space and can contribute to the success of future directed evolution experiments...
  30. pmc Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene
    Tjibbe Bosma
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Appl Environ Microbiol 68:3582-7. 2002
    ....
  31. ncbi request reprint Biocatalysis by dehalogenating enzymes
    Dick B Janssen
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Adv Appl Microbiol 61:233-52. 2007
  32. pmc Complete biodegradation of 4-fluorocinnamic acid by a consortium comprising Arthrobacter sp. strain G1 and Ralstonia sp. strain H1
    Syed A Hasan
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands
    Appl Environ Microbiol 77:572-9. 2011
    ..Degradation of 4-fluorobenzoic acid by strain H1 proceeded via 4-fluorocatechol, which was converted by an ortho-cleavage pathway...
  33. pmc Diversity and biocatalytic potential of epoxide hydrolases identified by genome analysis
    Bert van Loo
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Appl Environ Microbiol 72:2905-17. 2006
    ..The results show that the expression of epoxide hydrolase genes that are detected by analyses of genomic databases is a useful strategy for obtaining new biocatalysts...
  34. pmc The X-ray structure of the haloalcohol dehalogenase HheA from Arthrobacter sp. strain AD2: insight into enantioselectivity and halide binding in the haloalcohol dehalogenase family
    René M de Jong
    Laboratory of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Bacteriol 188:4051-6. 2006
    ..These results broaden the insight into the structural determinants that govern reactivity and selectivity in the haloalcohol dehalogenase family...
  35. ncbi request reprint Sequential kinetic resolution catalyzed by halohydrin dehalogenase
    Maja Majeric Elenkov
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Org Lett 8:4227-9. 2006
    ..8% ee (40% yield) and (S)-2 with 95.2% ee (41% yield). This reaction is carried out in aqueous solution under mild conditions and provides access to a useful statin side-chain building block...
  36. doi request reprint One pot 'click' reactions: tandem enantioselective biocatalytic epoxide ring opening and [3+2] azide alkyne cycloaddition
    Lachlan S Campbell-Verduyn
    Stratingh Institute for Chemistry, University of Groningen, Groningen, The Netherlands
    Chem Commun (Camb) 46:898-900. 2010
    ....
  37. pmc ADP competes with FAD binding in putrescine oxidase
    Erik W van Hellemond
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Biol Chem 283:28259-64. 2008
    ....
  38. ncbi request reprint Discovery of a eugenol oxidase from Rhodococcus sp. strain RHA1
    Jianfeng Jin
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    FEBS J 274:2311-21. 2007
    ..The ability to efficiently convert eugenol may facilitate biotechnological valorization of this natural aromatic compound...
  39. pmc Discovery of a novel styrene monooxygenase originating from the metagenome
    Erik W van Hellemond
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Appl Environ Microbiol 73:5832-9. 2007
    ..This excellent enantioselectivity in combination with the moderate sequence identity forms a clear indication that SmoA from a metagenomic origin represents a new enzyme within the small family of styrene monooxygenases...
  40. pmc Discovery and characterization of a putrescine oxidase from Rhodococcus erythropolis NCIMB 11540
    Erik W van Hellemond
    Laboratory of Biochemistry, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Appl Microbiol Biotechnol 78:455-63. 2008
    ..Mutation of this residue resulted in a drastic drop (five orders of magnitude) in catalytic efficiency. Interestingly, the mutant enzyme showed activity with monoamines, which are not accepted by wt-PuORh...
  41. ncbi request reprint Structural basis for the enantioselectivity of an epoxide ring opening reaction catalyzed by halo alcohol dehalogenase HheC
    René M de Jong
    Department of Biophysical Chemistry, University of Groningen, Nijenborgh 4, NL 9747 AG Groningen, The Netherlands
    J Am Chem Soc 127:13338-43. 2005
    ..This work represents a rare opportunity to explain the enantioselectivity of an enzymatic reaction by comparison of crystallographic data on the binding of both the favored and unfavored enantiomers...
  42. ncbi request reprint Biocatalytic conversion of epoxides
    Erik J de Vries
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Curr Opin Biotechnol 14:414-20. 2003
    ..The emerging new possibilities for enantioselective biocatalytic conversion of epoxides suggests that their importance in green chemistry will grow...
  43. doi request reprint Catalytic promiscuity of halohydrin dehalogenase and its application in enantioselective epoxide ring opening
    Ghannia Hasnaoui-Dijoux
    Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
    Chembiochem 9:1048-51. 2008
  44. ncbi request reprint Steady-state kinetics and tryptophan fluorescence properties of halohydrin dehalogenase from Agrobacterium radiobacter. Roles of W139 and W249 in the active site and halide-induced conformational change
    Lixia Tang
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biochemistry 42:14057-65. 2003
    ....
  45. ncbi request reprint Evolving haloalkane dehalogenases
    Dick B Janssen
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Curr Opin Chem Biol 8:150-9. 2004
    ..To improve enzymatic degradation of some environmentally important recalcitrant compounds, site-directed mutagenesis and directed-evolution studies are being done...
  46. ncbi request reprint Quantifying the accessibility of the metagenome by random expression cloning techniques
    Esther M Gabor
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Environ Microbiol 6:879-86. 2004
    ..Our study reveals significant differences in the predicted expression modes between distinct taxonomic groups of organisms and suggests that about 40% of the enzymatic activities may be readily recovered by random cloning in E. coli...
  47. ncbi request reprint Molecular dynamics simulations as a tool for improving protein stability
    Mariel G Pikkemaat
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747AG, The Netherlands
    Protein Eng 15:185-92. 2002
    ..The results show that MD simulations are capable of identifying mobile protein domains that can successfully be used as a target for stability enhancement by the introduction of a disulfide cross-link...
  48. ncbi request reprint Improved catalytic properties of halohydrin dehalogenase by modification of the halide-binding site
    Lixia Tang
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Biochemistry 44:6609-18. 2005
    ..Taken together, these results show that the disruption of two hydrogen bonds around the halide-binding site increases the rate of halide release and can enhance the overall catalytic activity of HheC...
  49. ncbi request reprint Discovery of a thermostable Baeyer-Villiger monooxygenase by genome mining
    Marco W Fraaije
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Appl Microbiol Biotechnol 66:393-400. 2005
    ..This study demonstrates that, using effective annotation tools, genomes can efficiently be exploited as a source of novel BVMOs...
  50. ncbi request reprint Crystallization and preliminary X-ray analysis of an enantioselective halohydrin dehalogenase from Agrobacterium radiobacter AD1
    René M de Jong
    Laboratory of Biophysical Chemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Acta Crystallogr D Biol Crystallogr 58:176-8. 2002
    ..5, c = 121.4 A, and contain two monomers in the asymmetric unit. The crystals diffract to 3.0 A resolution with X-rays from a Cu Kalpha rotating-anode generator...
  51. ncbi request reprint Kinetic mechanism and enantioselectivity of halohydrin dehalogenase from Agrobacterium radiobacter
    Lixia Tang
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, NL 9747 AG, Groningen, The Netherlands
    Biochemistry 42:5378-86. 2003
    ..The high enantiopreference for the (R)-enantiomer can be explained by weak substrate binding of the (S)-enantiomer and a lower rate of reaction at the active site...
  52. ncbi request reprint Formation of enantiopure 5-substituted oxazolidinones through enzyme-catalysed kinetic resolution of epoxides
    Maja Majerić Elenkov
    Ruder Boskovic Institute, Zagreb, Croatia
    Org Lett 10:2417-20. 2008
    ..This is the first example of the biocatalytic conversion of a range of epoxides to the corresponding oxazolidinones...
  53. ncbi request reprint Identification of the catalytic residues of alpha-amino acid ester hydrolase from Acetobacter turbidans by labeling and site-directed mutagenesis
    Jolanda J Polderman-Tijmes
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, NL 9747 AG Groningen, The Netherlands
    J Biol Chem 277:28474-82. 2002
    ..This distinguishes the alpha-amino acid ester hydrolase from the Ntn-hydrolase family of beta-lactam antibiotic acylases...
  54. ncbi request reprint The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase
    Wynand B L Alkema
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Eur J Biochem 269:2093-100. 2002
    ..The reduced specificity for the product and the high initial transferase/hydrolase ratio of betaF24A resulted in high yields in acyl transfer reactions...
  55. pmc Role of alphaArg145 and betaArg263 in the active site of penicillin acylase of Escherichia coli
    Wynand B L Alkema
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biochem J 365:303-9. 2002
    ....
  56. doi request reprint Inactivation of epoxide hydrolase by catalysis-induced formation of isoaspartate
    Bert van Loo
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    FEBS Lett 582:1581-6. 2008
    ..Inactivation occurred at a lower rate with the (S)-enantiomer of para-nitrostyrene epoxide, indicating that it is related to the structure of the covalent hydroxyalkyl-enzyme intermediate...
  57. ncbi request reprint The prodrug activator EtaA from Mycobacterium tuberculosis is a Baeyer-Villiger monooxygenase
    Marco W Fraaije
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Biol Chem 279:3354-60. 2004
    ..This reactivity and substrate acceptance-profiling study provides valuable information concerning this newly identified prodrug activator from M. tuberculosis...
  58. pmc Substrate specificity and enantioselectivity of 4-hydroxyacetophenone monooxygenase
    Nanne M Kamerbeek
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
    Appl Environ Microbiol 69:419-26. 2003
    ..The biocatalytic properties of HAPMO described here show the potential of this enzyme for biotechnological applications...
  59. ncbi request reprint Changing the substrate specificity of a chitooligosaccharide oxidase from Fusarium graminearum by model-inspired site-directed mutagenesis
    Dominic P H M Heuts
    Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    FEBS Lett 581:4905-9. 2007
    ..The substrate specificity of wild type ChitO and the Q268R mutant were examined and confirmed that Q268 is indeed involved in N-acetyl recognition...
  60. ncbi request reprint Coenzyme binding during catalysis is beneficial for the stability of 4-hydroxyacetophenone monooxygenase
    Robert H H van den Heuvel
    Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
    J Biol Chem 280:32115-21. 2005
    ..The interaction with the coenzyme analog 3-aminopyridine adenine dinucleotide phosphate (AADP(+)) strongly enhanced the thermal stability of wild type HAPMO. This coenzyme-induced stabilization may also be important for related enzymes...
  61. ncbi request reprint Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of beta-lactam antibiotics
    Wynand B L Alkema
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    Eur J Biochem 270:3675-83. 2003
    ..By combining the results of the steady-state, presteady state and nucleophile binding experiments, values for the relevant kinetic constants for the synthesis and hydrolysis of beta-lactam antibiotics were obtained...
  62. ncbi request reprint Catalytic mechanism of the maloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26
    Zbynek Prokop
    National Centre for Biomolecular Research, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic
    J Biol Chem 278:45094-100. 2003
    ....
  63. ncbi request reprint Metabolism of mono- and dihalogenated C1 and C2 compounds by Xanthobacter autotrophicus growing on 1,2-dichloroethane
    Maciej Torz
    Institute of Chemical Engineering, Bulgarian Academy of Sciences, Acad G Bonchev Str, Bl 103, 113, Sofia, Bulgaria
    Biodegradation 18:145-57. 2007
    ..Our results extend the spectrum of halogenated C1 and C2 compounds that are known to be degraded by strain GJ10 and provide information on toxic effects and transformation of compounds not serving as a carbon source for this bacterium...
  64. ncbi request reprint Directed evolution of epoxide hydrolase from A. radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling
    Bert van Loo
    Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Chem Biol 11:981-90. 2004
    ..Several mutations in the shuffled mutants had additive effects...
  65. ncbi request reprint Increasing the synthetic performance of penicillin acylase PAS2 by structure-inspired semi-random mutagenesis
    Esther M Gabor
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Protein Eng Des Sel 17:571-9. 2004
    ..Although catalytic activity dropped by a factor of 5-10, the enhanced synthetic performance of the recovered penicillin acylase variants makes them interesting biocatalysts for the production of beta-lactam antibiotics...
  66. ncbi request reprint Acetobacter turbidans alpha-amino acid ester hydrolase: how a single mutation improves an antibiotic-producing enzyme
    Thomas R M Barends
    Laboratories of Biophysical Chemistry and Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Biol Chem 281:5804-10. 2006
    ..Because this changes the properties of the beta-lactam binding site, this could explain the increased beta-lactam transferase activity of this mutant...
  67. pmc Degradation of fluorobenzene by Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol
    Maria F Carvalho
    Escola Superior de Biotecnologia, Universidade Catolica Portuguesa, Rua Dr Antonio Bernardino de Almeida, 4200 072 Porto, Portugal
    Appl Environ Microbiol 72:7413-7. 2006
    ..The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol...