Ad C Fluit

Summary

Affiliation: University Medical Center
Country: The Netherlands

Publications

  1. ncbi Prevalence of aminoglycoside resistance in 20 European university hospitals participating in the European SENTRY Antimicrobial Surveillance Programme
    F J Schmitz
    Eijkman Winkler Institute for Clinical Microbiology, Utrecht University, The Netherlands
    Eur J Clin Microbiol Infect Dis 18:414-21. 1999
  2. ncbi Beta-lactam susceptibilities and prevalence of ESBL-producing isolates among more than 5000 European Enterobacteriaceae isolates
    S Nijssen
    Department of Internal Medicine, Division of Emergency Medicine and Infectious Diseases, F 02 126, University Medical Center Utrecht, P O Box 85000, 3508 GA Utrecht, The Netherlands
    Int J Antimicrob Agents 24:585-91. 2004
  3. pmc In vitro activity of daptomycin against gram-positive European clinical isolates with defined resistance determinants
    Ad C Fluit
    Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    Antimicrob Agents Chemother 48:1007-11. 2004
  4. ncbi Bacterial resistance in urinary tract infections: how to stem the tide
    A C Fluit
    Eijkman Winkler Institute, University Medical Center Utrecht, Utrecht, The Netherlands
    Expert Opin Pharmacother 2:813-8. 2001
  5. ncbi Frequency of isolation and antimicrobial resistance of gram-negative and gram-positive bacteria from patients in intensive care units of 25 European university hospitals participating in the European arm of the SENTRY Antimicrobial Surveillance Program 19
    A C Fluit
    Eijkman Winkler Institute, University Medical Center Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 20:617-25. 2001
  6. ncbi Frequency of isolation of pathogens from bloodstream, nosocomial pneumonia, skin and soft tissue, and urinary tract infections occurring in European patients
    A C Fluit
    Eijkman Winkler Institute, University Hospital Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 20:188-91. 2001
  7. ncbi Multi-resistance to antimicrobial agents for the ten most frequently isolated bacterial pathogens
    A C Fluit
    Eijkman Winkler Institute, University Hospital Utrecht, Room G04 614, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Int J Antimicrob Agents 18:147-60. 2001
  8. pmc Molecular detection of antimicrobial resistance
    A C Fluit
    Eijkman Winkler Institute, University Medical Center Utrecht, Utrecht, The Netherlands
    Clin Microbiol Rev 14:836-71, table of contents. 2001
  9. ncbi Daptomycin in vitro susceptibility in European Gram-positive clinical isolates
    Ad C Fluit
    Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Medical Center Utrecht, 3504 GA Utrecht, The Netherlands
    Int J Antimicrob Agents 24:59-66. 2004
  10. ncbi Susceptibility of European beta-lactamase-positive and -negative Haemophilus influenzae isolates from the periods 1997/1998 and 2002/2003
    A C Fluit
    Eijkman Winkler Center, University Medical Center Utrecht, The Netherlands
    J Antimicrob Chemother 56:133-8. 2005

Detail Information

Publications104 found, 100 shown here

  1. ncbi Prevalence of aminoglycoside resistance in 20 European university hospitals participating in the European SENTRY Antimicrobial Surveillance Programme
    F J Schmitz
    Eijkman Winkler Institute for Clinical Microbiology, Utrecht University, The Netherlands
    Eur J Clin Microbiol Infect Dis 18:414-21. 1999
    ..The reason for this observation is unclear, although changes in antibiotic prescribing patterns that result in increased selective pressure from gentamicin may have contributed to these increased rates of aminoglycoside resistance...
  2. ncbi Beta-lactam susceptibilities and prevalence of ESBL-producing isolates among more than 5000 European Enterobacteriaceae isolates
    S Nijssen
    Department of Internal Medicine, Division of Emergency Medicine and Infectious Diseases, F 02 126, University Medical Center Utrecht, P O Box 85000, 3508 GA Utrecht, The Netherlands
    Int J Antimicrob Agents 24:585-91. 2004
    ..5% of all isolates). ESBL-production was confirmed with the ESBL-Etest and disk diffusion test in 1.3% of Escherichia coli isolates, 18.4% of Klebsiella pneumoniae, 12.6% of Klebsiella oxytoca and 5.3% of Proteus mirabilis isolates...
  3. pmc In vitro activity of daptomycin against gram-positive European clinical isolates with defined resistance determinants
    Ad C Fluit
    Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    Antimicrob Agents Chemother 48:1007-11. 2004
    ..03 to 1, 0.25 to 8, 0.12 to 1, and 0.06 to 8 micro g/ml, respectively. For only one streptococcus isolate and seven enterococcus isolates was the MIC 8 micro g/ml...
  4. ncbi Bacterial resistance in urinary tract infections: how to stem the tide
    A C Fluit
    Eijkman Winkler Institute, University Medical Center Utrecht, Utrecht, The Netherlands
    Expert Opin Pharmacother 2:813-8. 2001
    ..Therefore, we believe, that we can reduce the tide but we will still have to deal with the resistance problem in the future...
  5. ncbi Frequency of isolation and antimicrobial resistance of gram-negative and gram-positive bacteria from patients in intensive care units of 25 European university hospitals participating in the European arm of the SENTRY Antimicrobial Surveillance Program 19
    A C Fluit
    Eijkman Winkler Institute, University Medical Center Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 20:617-25. 2001
    ..However, the situation for patients with Pseudomonas aeruginosa infections is critical...
  6. ncbi Frequency of isolation of pathogens from bloodstream, nosocomial pneumonia, skin and soft tissue, and urinary tract infections occurring in European patients
    A C Fluit
    Eijkman Winkler Institute, University Hospital Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 20:188-91. 2001
    ..5% and 37.4% of isolates, respectively). In addition, Pseudomonas aeruginosa played an important role in all types of infection analyzed...
  7. ncbi Multi-resistance to antimicrobial agents for the ten most frequently isolated bacterial pathogens
    A C Fluit
    Eijkman Winkler Institute, University Hospital Utrecht, Room G04 614, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Int J Antimicrob Agents 18:147-60. 2001
    ..influenzae and M. catarrhalis. Cross- and multi-resistance are important problems for Gram-negative and Gram-positive bacteria but not for fastidious bacteria with the exception of penicillin-resistant S. pneumoniae...
  8. pmc Molecular detection of antimicrobial resistance
    A C Fluit
    Eijkman Winkler Institute, University Medical Center Utrecht, Utrecht, The Netherlands
    Clin Microbiol Rev 14:836-71, table of contents. 2001
    ..In this review, we describe the applications of molecular techniques for the detection of antimicrobial resistance and the current state of the art...
  9. ncbi Daptomycin in vitro susceptibility in European Gram-positive clinical isolates
    Ad C Fluit
    Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Medical Center Utrecht, 3504 GA Utrecht, The Netherlands
    Int J Antimicrob Agents 24:59-66. 2004
    ..aureus and group A streptococci, all isolates tested were susceptible to daptomycin. Based on a breakpoint of 4 mg/L for vancomcyin-susceptible E. faecalis 99.7% of these isolates were susceptible to daptomycin...
  10. ncbi Susceptibility of European beta-lactamase-positive and -negative Haemophilus influenzae isolates from the periods 1997/1998 and 2002/2003
    A C Fluit
    Eijkman Winkler Center, University Medical Center Utrecht, The Netherlands
    J Antimicrob Chemother 56:133-8. 2005
    ....
  11. pmc Presence of tetracycline resistance determinants and susceptibility to tigecycline and minocycline
    Ad C Fluit
    Eijkman Winkler Institute, University Medical Center Utrecht, Room G04 614, P O Box 85500, 3508 GA Utrecht, The Netherlands
    Antimicrob Agents Chemother 49:1636-8. 2005
    ....
  12. ncbi Towards more virulent and antibiotic-resistant Salmonella?
    Ad C Fluit
    Eijkman Winkler Institute, University Medical Center Utrecht, P O Box 85500, 3508 GA Utrecht, Utrecht, The Netherlands
    FEMS Immunol Med Microbiol 43:1-11. 2005
    ..These newly formed plasmids can be selected by antibiotic pressure and thereby for virulence factors. Taken together these trends may lead to more virulent and antibiotic-resistant Salmonella...
  13. ncbi In vitro activity of AZD2563, a novel oxazolidinone, against European Gram-positive cocci
    A C Fluit
    Eijkman Winkler Center, UMCU, Room G04 614, PO Box 85500, 3508 GA Utrecht, The Netherlands
    J Antimicrob Chemother 50:271-6. 2002
    ..Thus, this oxazolidinone is a promising new antibiotic for the treatment of infections with Gram-positive cocci...
  14. ncbi Resistance integrons and super-integrons
    A C Fluit
    Eijkman Winkler Center, P O Box 85500, 3508 GA Utrecht, The Netherlands
    Clin Microbiol Infect 10:272-88. 2004
    ..Many of these encode resistance against newer antibiotics such as cephalosporins and carbapenems. Resistance integrons have been found in isolates from a wide variety of sources, including food...
  15. ncbi Antimicrobial resistance in European isolates of Pseudomonas aeruginosa. European SENTRY Participants
    A C Fluit
    Eijkman Winkler Institute, University Medical Center Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 19:370-4. 2000
    ..Isolates that were resistant to one class of antibiotics were also often resistant to at least one other class of antibiotics. Imipenem-resistant isolates were generally not clonally related...
  16. ncbi Antimicrobial resistance among community-acquired pneumonia isolates in Europe: first results from the SENTRY antimicrobial surveillance program 1997. SENTRY Participants Group
    A C Fluit
    Eijkman Winkler Institute for Clinical Microbiology, University Hospital Utrecht, Utrecht, The Netherlands
    Int J Infect Dis 3:153-6. 1999
    ..The SENTRY antimicrobial surveillance program was established to monitor the occurrence and antimicrobial susceptibility of bacterial pathogens via an international network of sentinel hospitals...
  17. ncbi Antimicrobial resistance among urinary tract infection (UTI) isolates in Europe: results from the SENTRY Antimicrobial Surveillance Program 1997
    A C Fluit
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Hospital Utrecht, The Netherlands
    Antonie Van Leeuwenhoek 77:147-52. 2000
    ..Enterococcus spp. showed the highest susceptibility to vancomycin (98%), teicoplanin (98%), and ampicillin (94%)...
  18. ncbi Class 1 integrons, gene cassettes, mobility, and epidemiology
    A C Fluit
    Eijkman Winkler Institute, University Hospital Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 18:761-70. 1999
    ..The aim of this review is to describe the versatility of integrons, especially their mobility and their ability to collect resistance genes...
  19. ncbi Antimicrobial susceptibility and frequency of occurrence of clinical blood isolates in Europe from the SENTRY antimicrobial surveillance program, 1997 and 1998
    A C Fluit
    Eijkman Winkler Institute for Clinical Microbiology, University Hospital Utrecht, 3584CX Utrecht, The Netherlands
    Clin Infect Dis 30:454-60. 2000
    ..However, extrapolation of these data to smaller and nonteaching hospitals should be undertaken with caution, since resistance rates may be lower in these facilities...
  20. pmc Antimicrobial resistance, class 1 integrons, and genomic island 1 in Salmonella isolates from Vietnam
    An T T Vo
    Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    PLoS ONE 5:e9440. 2010
    ..The objective was to investigate the phenotypic and genotypic resistance and the horizontal transfer of resistance determinants from Salmonella isolates from humans and animals in Vietnam...
  21. pmc Enterobacter cloacae outbreak and emergence of quinolone resistance gene in Dutch hospital
    Armand Paauw
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Emerg Infect Dis 12:807-12. 2006
    ..On the same plasmid, 2 other class 1 integrons were present. One was a new integron associated with the bla(CTX-M-9) extended-spectrum beta-lactamase...
  22. pmc Failure to control an outbreak of qnrA1-positive multidrug-resistant Enterobacter cloacae infection despite adequate implementation of recommended infection control measures
    Armand Paauw
    Eijkman Winkler Center for Microbiology, Infectious Diseases and Inflammation, Department of Hospital Hygiene and Infection Prevention, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Microbiol 45:1420-5. 2007
    ..Strategies to identify such strains in an early phase and adapted guidelines for such "superbugs" are needed to prevent these clones from becoming endemic...
  23. pmc Extensive hospital-wide spread of a multidrug-resistant enterobacter cloacae clone, with late detection due to a variable antibiogram and frequent patient transfer
    Maurine A Leverstein-van Hall
    Eijkman Winkler Institute for Microbiology, Infectious Disease and Inflammation, University Hospital Utrecht, Rm G 04 614, PO 855000, 3508 GA Utrecht, The Netherlands
    J Clin Microbiol 44:518-24. 2006
    ..These findings suggest that computerized tools for surveillance may recognize resistance trends that are too complex to be detected by manual review and indicate the need for prospective evaluation of such algorithms...
  24. doi Identification of resistance and virulence factors in an epidemic Enterobacter hormaechei outbreak strain
    Armand Paauw
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Microbiology 155:1478-88. 2009
    ..The high number of genes detected in the EHOS that were related to transferable elements reflects the genomic plasticity of the E. cloacae complex and may explain the emergence of the EHOS in the hospital environment...
  25. pmc Genomic diversity within the Enterobacter cloacae complex
    Armand Paauw
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    PLoS ONE 3:e3018. 2008
    ..Identification of species based on currently available genotyping tools is already superior to phenotypic identification, but the taxonomy of isolates belonging to this complex is cumbersome...
  26. doi Acquisition and cross-transmission of Staphylococcus aureus in European intensive care units
    Alexander L A Bloemendaal
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    Infect Control Hosp Epidemiol 30:117-24. 2009
    ..To study the acquisition and cross-transmission of Staphylococcus aureus in different intensive care units (ICUs)...
  27. doi Identical plasmid AmpC beta-lactamase genes and plasmid types in E. coli isolates from patients and poultry meat in the Netherlands
    Guido M Voets
    Department of Medical Microbiology, University Medical Centre Utrecht, G04 614, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Int J Food Microbiol 167:359-62. 2013
    ..These findings imply that a foodborne transmission route of blaCMY-2 harbouring plasmids cannot be excluded and that further evaluation is required...
  28. ncbi A novel Salmonella genomic island 1 and rare integron types in Salmonella Typhimurium isolates from horses in The Netherlands
    An T T Vo
    Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Antimicrob Chemother 59:594-9. 2007
    ..To investigate the genotypic resistance of integron-carrying Salmonella Typhimurium isolates from horses and their genetic relationship...
  29. ncbi Class 1 integrons in Dutch Salmonella enterica serovar Dublin isolates from clinical cases of bovine salmonellosis
    An T T Vo
    Bacteriology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    Vet Microbiol 117:192-200. 2006
    ..Thus, the Dutch Salmonella Dublin strains were closely related but not clonal...
  30. pmc Determination of the relationship between group A streptococcal genome content, M type, and toxic shock syndrome by a mixed genome microarray
    Bart J M Vlaminckx
    University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Infect Immun 75:2603-11. 2007
    ..Finally, in contrast to M-type-specific virulence profiles, we did not identify a common virulence profile among strains associated with TSS irrespective of their M type...
  31. pmc Evolution in quantum leaps: multiple combinatorial transfers of HPI and other genetic modules in Enterobacteriaceae
    Armand Paauw
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    PLoS ONE 5:e8662. 2010
    ..Multiple combinatorial transfers of diverse combinations of the HPI and other genomic elements among Enterobacteriaceae may accelerate the generation of new pathogenic strains...
  32. pmc Population distribution of Beta-lactamase conferring resistance to third-generation cephalosporins in human clinical Enterobacteriaceae in the Netherlands
    Guido M Voets
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    PLoS ONE 7:e52102. 2012
    ..Our findings demonstrate that bla(CTX-M-15) is the most prevalent ESBL and we report a larger than previously reported prevalence of ampC genes among Enterobacteriaceae responsible for resistance to third-generation cephalosporins...
  33. ncbi Is methicillin-resistant Staphylococcus aureus more contagious than methicillin-susceptible S. aureus in a surgical intensive care unit?
    Menno R Vriens
    Department of Surgery, Eijkman Winkler Institute, University Medical Center, Utrecht, The Netherlands
    Infect Control Hosp Epidemiol 23:491-4. 2002
    ..We were surprised by the epidemic behavior of these strains and wondered whether this was exceptional for S. aureus or whether methicillin-susceptible S. aureus (MSSA) was also spreading in the ICU...
  34. ncbi Antibiotic rotation and development of gram-negative antibiotic resistance
    Harald J van Loon
    Department of Surgery, Eijkman Winkler Institute for Microbiology, Infectious Disease and Inflammation, University Medical Center Utrecht, Heidelberglaan 100, PO Box 3508 GA, Utrecht, The Netherlands
    Am J Respir Crit Care Med 171:480-7. 2005
    ..Cycling of homogeneous antibiotic exposure is unlikely to control the emergence of gram-negative antimicrobial resistance in intensive care units...
  35. pmc Site-specific manifestations of invasive group a streptococcal disease: type distribution and corresponding patterns of virulence determinants
    Bart J M Vlaminckx
    Eijkman Winkler Institute for Medical Microbiology, Infectious Diseases and Inflammation, University Hospital Utrecht, Utrecht, Germany
    J Clin Microbiol 41:4941-9. 2003
    ..M1 isolates comprised a single clone carrying highly mitogenic toxin genes (speA, smeZ) and were associated with toxic shock-like syndrome. Toxin profiles were highly conserved among the most virulent strains, such as M1 and M3...
  36. pmc Methicillin-resistant coagulase-negative staphylococci on pig farms as a reservoir of heterogeneous staphylococcal cassette chromosome mec elements
    Pawel Tulinski
    Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    Appl Environ Microbiol 78:299-304. 2012
    ..We conclude that staphylococci on pig farms act as a reservoir of heterogeneous SCCmec elements. These staphylococci may act as a source for transfer of SCCmec to S. aureus...
  37. doi Colonization and transmission of meticillin-susceptible and meticillin-resistant Staphylococcus aureus in a murine nasal colonization model
    Alexander L A Bloemendaal
    Department of Medical Microbiology, University Medical Center Utrecht, The Netherlands
    J Med Microbiol 60:812-6. 2011
    ..These results suggest a more rapid spread of MSSA compared to MRSA. However, MRSA shows a more stable nasal colonization after a longer period of time...
  38. pmc Evaluation of the Etest ESBL and the BD Phoenix, VITEK 1, and VITEK 2 automated instruments for detection of extended-spectrum beta-lactamases in multiresistant Escherichia coli and Klebsiella spp
    Maurine A Leverstein-van Hall
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Center Utrecht, Room G04 614, PO Box 85000, 3508 GA Utrecht, The Netherlands
    J Clin Microbiol 40:3703-11. 2002
    ....
  39. pmc Genetic variation in spatio-temporal confined USA300 community-associated MRSA isolates: a shift from clonal dispersion to genetic evolution?
    Neeltje Carpaij
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    PLoS ONE 6:e16419. 2011
    ....
  40. pmc Methicillin resistance transfer from Staphylocccus epidermidis to methicillin-susceptible Staphylococcus aureus in a patient during antibiotic therapy
    Alexander L A Bloemendaal
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 5:e11841. 2010
    ..epidermidis had transferred SCCmec to the MSSA to become MRSA. The aim of this study was to show that these isolates form an isogenic pair and that interspecies horizontal SCCmec transfer occurred...
  41. ncbi Integrons in Escherichia coli from food-producing animals in The Netherlands
    Adrienne T A Box
    Eijkman Winkler Institute for Microbiology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Microb Drug Resist 11:53-7. 2005
    ..Common integron types indicate that antibiotic resistance genes are exchanged via the food chain between different reservoirs of both human and animal origin...
  42. ncbi Toxin-gene profile heterogeneity among endemic invasive European group A streptococcal isolates
    Franz Josef Schmitz
    Eijkman Winkler Institute for Medical Microbiology, Infectious Diseases and Inflammation, University Hospital Utrecht, Utrecht, The Netherlands
    J Infect Dis 188:1578-86. 2003
    ..This could indicate that M proteins function, directly or indirectly, as barriers for horizontal gene exchange...
  43. pmc Novel multiple-locus variable-number tandem-repeat analysis method for rapid molecular typing of human Staphylococcus aureus
    Risma Ikawaty
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    J Clin Microbiol 46:3147-51. 2008
    ..MLVA correctly assigned isolates to outbreaks or identified isolates as unlinked. MLVA is a rapid and simple method for the epidemiological typing of S. aureus...
  44. ncbi Multidrug resistance among Enterobacteriaceae is strongly associated with the presence of integrons and is independent of species or isolate origin
    Maurine A Leverstein-van Hall
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, Utrecht, The Netherlands
    J Infect Dis 187:251-9. 2003
    ..The acquisition of resistance genes is not random, and the transfer of integron-carrying elements plays a dominant role in the development of MDR by Enterobacteriaceae...
  45. pmc Epidemiology and susceptibility of 3,051 Staphylococcus aureus isolates from 25 university hospitals participating in the European SENTRY study
    A C Fluit
    Eijkman Winkler Institute for Microbiology, University Medical Center, Utrecht, The Netherlands
    J Clin Microbiol 39:3727-32. 2001
    ..MRSA isolates appeared to be more prevalent in intensive care units than in outpatient departments. Only a small minority of MSSA isolates were multidrug resistant, whereas the majority of MRSA isolates were multidrug resistant...
  46. pmc mecA gene is widely disseminated in Staphylococcus aureus population
    C L C Wielders
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, 3584 CX Utrecht, The Netherlands
    J Clin Microbiol 40:3970-5. 2002
    ..This supports the hypothesis that horizontal transfer plays an important role in the dissemination of the mecA gene in the S. aureus population...
  47. doi Detection of carbapenemase-producing Enterobacteriaceae with a commercial DNA microarray
    James Cohen Stuart
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    J Med Microbiol 61:809-12. 2012
    ..In conclusion, the CDT102 microarray is a rapid and accurate tool for the detection of carbapenemase and ESBL genes, although the array seems less suitable for epidemiology of ESBL genes...
  48. pmc Methicillin-resistant Staphylococcus aureus in pigs with exudative epidermitis
    Engeline van Duijkeren
    Utrecht University, Utrecht, The Netherlands
    Emerg Infect Dis 13:1408-10. 2007
    ..The MRSA strains were indistinguishable, suggesting direct transmission...
  49. pmc Operon structure of Staphylococcus aureus
    Nicole J P ten Broeke-Smits
    Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    Nucleic Acids Res 38:3263-74. 2010
    ..This is the first experimental approach to fully identify operon structures in S. aureus. It forms the basis for further in vitro regulation studies that will profoundly advance the understanding of bacterial pathophysiology in vivo...
  50. ncbi Outbreak of a susceptible strain of Acinetobacter species 13 (sensu Tjernberg and Ursing) in an adult neurosurgical intensive care unit
    H van Dessel
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, Utrecht, The Netherlands
    J Hosp Infect 51:89-95. 2002
    ..Correct identification to the species level of Acinetobacter by genotypic methods is necessary to get insight in the importance of the different Acinetobacter genomic species in hospital epidemiology...
  51. ncbi Evaluation of genetic determinants involved in beta-lactam- and multiresistance in a surgical ICU
    H J van Loon
    Eijkman Winkler Institute for Microbiology, Infectious Disease and Inflammation, University Medical Center Utrecht, Utrecht, The Netherlands
    Int J Antimicrob Agents 24:130-4. 2004
    ..It can be concluded that integrons and associated resistance determinants played only a minor role in the surgical ICU and beta-lactam resistance among P. aeruginosa isolates was most likely due to the derepression of its AmpC gene...
  52. ncbi Comparative activity of 27 antimicrobial compounds against 698 Streptococcus pneumoniae isolates originating from 20 European university hospitals. SENTRY Participants Group
    F J Schmitz
    Eijkman Winkler Institute for Clinical Microbiology, Utrecht University, The Netherlands
    Eur J Clin Microbiol Infect Dis 18:450-3. 1999
    ..The results of the current surveillance study confirm the ongoing trend among European clinical pneumococcal isolates of decreased sensitivity to various antibiotics...
  53. pmc An ex vivo porcine nasal mucosa explants model to study MRSA colonization
    Pawel Tulinski
    Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    PLoS ONE 8:e53783. 2013
    ..No morphological changes in the epithelium layer were observed during the incubation with MRSA. We conclude that porcine nasal mucosa explants are a valuable ex vivo model to unravel the interaction of MRSA with nasal tissue...
  54. ncbi Evidence of extensive interspecies transfer of integron-mediated antimicrobial resistance genes among multidrug-resistant Enterobacteriaceae in a clinical setting
    Maurine A Leverstein-van Hall
    Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    J Infect Dis 186:49-56. 2002
    ..These findings provide strong evidence that the horizontal transfer of resistance genes contributed largely to the emergence of multidrug-resistant Enterobacteriaceae in this clinical setting...
  55. pmc Human-to-dog transmission of methicillin-resistant Staphylococcus aureus
    Engeline van Duijkeren
    Faculty of Veterinary Medicine, Utrecht, The Netherlands
    Emerg Infect Dis 10:2235-7. 2004
    ..Pulsed-field gel electrophoresis and typing of the staphylococcal chromosome cassette mec (SCCmec) region showed that both MRSA strains were identical...
  56. ncbi Distribution of macrolide-resistance genes in Staphylococcus aureus blood-culture isolates from fifteen German university hospitals. M.A.R.S. Study Group. Multicentre Study on Antibiotic Resistance in Staphylococci
    F J Schmitz
    Eijkman Winkler Institute for Clinical Microbiology, Utrecht Medical Center, University of Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 19:385-7. 2000
    ..2%) and ereA (1/134; 0.7%). Overall, resistance to erythromycin was predominantly due to the presence of two erm genes, although with different distributions, depending on the methicillin-resistance pattern...
  57. doi Characterization of Dutch Staphylococcus aureus from bovine mastitis using a Multiple Locus Variable Number Tandem Repeat Analysis
    Risma Ikawaty
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Vet Microbiol 136:277-84. 2009
    ..PFGE, MLST, and MLVA are adequate typing methods for bovine-associated S. aureus...
  58. doi Evaluation of a commercial microarray as a confirmation test for the presence of extended-spectrum β-lactamases in isolates from the routine clinical setting
    T N Platteel
    SALTRO, Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Clin Microbiol Infect 17:1435-8. 2011
    ..Using sequencing as the reference method the sensitivity of the microarray was 97% (237/245), the specificity 98% (97/99), the positive predictive value 99% (237/239) and the negative predictive value 92% (97/105)...
  59. doi Effect of long-term trimethoprim/sulfamethoxazole treatment on resistance and integron prevalence in the intestinal flora: a randomized, double-blind, placebo-controlled trial in children
    Erwin L van der Veen
    Department of Otorhinolaryngology, Wilhelmina Children s Hospital, University Medical Center Utrecht, The Netherlands
    J Antimicrob Chemother 63:1011-6. 2009
    ..The aim of this study was to test the hypothesis that trimethoprim/sulfamethoxazole selects for integron-positive and multidrug-resistant Enterobacteriaceae in the intestinal flora...
  60. ncbi Clinical evaluation of a NASBA-based assay for detection of Candida spp. in blood and blood cultures
    Annemarie Borst
    Eijkman Winkler Center for Microbiology, Infectious Diseases and Inflammation, University Medical Center, Utrecht, The Netherlands
    Clin Lab 48:487-92. 2002
    ..The NASBA assay detected Candida RNA in three patients. In one patient, the yeast was not detected by automated blood culturing, in another patient the NASBA assay detected the infection two days earlier than the blood culture system...
  61. pmc Association of alterations in ParC and GyrA proteins with resistance of clinical isolates of Enterococcus faecium to nine different fluoroquinolones
    S Brisse
    Eijkman Winkler Institute, Utrecht University, 3584 CX, Utrecht, The Netherlands
    Antimicrob Agents Chemother 43:2513-6. 1999
    ..For all isolates, clinafloxacin and sitafloxacin showed the best activities...
  62. ncbi Bacterial resistance: a sensitive issue complexity of the challenge and containment strategy in Europe
    W T M Jansen
    Eijkman Winkler Institute, University Medical Center Utrecht, UMCU G04 614, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Drug Resist Updat 9:123-33. 2006
    ..This review discusses the complexity of resistance, identifies its main drivers and proposes measures to contain it on a European scale...
  63. ncbi Control of nosocomial multiresistant Enterobacteriaceae using a temporary restrictive antibiotic agent policy
    M A Leverstein-Van Hall
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 20:785-91. 2001
    ..In the years since, the incidence has remained at this low level, and the antibiotic costs have decreased to a level lower than before intervention...
  64. ncbi Cloning and characterization of an accessory gene regulator (agr)-like locus from Staphylococcus epidermidis
    W J Van Wamel
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Netherlands
    FEMS Microbiol Lett 163:1-9. 1998
    ..epidermidis A097, which showed a delayed agr-response, revealed a truncated AgrC lacking the histidine kinase domain. These data indicate that an agr-like locus is active in S. epidermidis during the post-exponential phase...
  65. ncbi Detection of Candida spp. in blood cultures using nucleic acid sequence-based amplification (NASBA)
    A Borst
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Center, Utrecht, The Netherlands
    Diagn Microbiol Infect Dis 39:155-60. 2001
    ..In summary, using NASBA for the detection of yeast RNA in blood cultures, we have shown for the first time that it is possible to improve the detection rate of yeasts in blood cultures by using amplification technology...
  66. pmc Molecular surveillance of European quinolone-resistant clinical isolates of Pseudomonas aeruginosa and Acinetobacter spp. using automated ribotyping
    S Brisse
    Eijkman Winkler Institute, Utrecht University, 3584 CX Utrecht, The Netherlands
    J Clin Microbiol 38:3636-45. 2000
    ....
  67. pmc Sex-limited protein: in vitro and in vivo functions
    F J Beurskens
    Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, Utrecht University Hospital, Utrecht, The Netherlands
    Clin Exp Immunol 116:395-400. 1999
    ..Our results imply that Slp is a mouse complement component with functions which partially resemble some of those of human C4A...
  68. pmc Comparative in vitro activities of ciprofloxacin, clinafloxacin, gatifloxacin, levofloxacin, moxifloxacin, and trovafloxacin against Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter cloacae, and Enterobacter aerogenes clinical isolates with alterat
    S Brisse
    Eijkman Winkler Institute, Utrecht University, 3584 CX, Utrecht, The Netherlands
    Antimicrob Agents Chemother 43:2051-5. 1999
    ..Irrespective of the alterations in GyrA and ParC proteins, clinafloxacin exhibited greater activity than all other fluoroquinolones tested against K. pneumoniae and E. aerogenes...
  69. pmc In vitro activities of sitafloxacin (DU-6859a) and six other fluoroquinolones against 8,796 clinical bacterial isolates
    D Milatovic
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Hospital Utrecht, Utrecht, The Netherlands
    Antimicrob Agents Chemother 44:1102-7. 2000
    ..Sitafloxacin was the most active agent against gram-positive cocci and anaerobes. Against Enterobacteriaceae and nonfermenters, its activity was either equivalent to or better than that of clinafloxacin...
  70. ncbi Epidemiology of quinolone resistance of Klebsiella pneumoniae and Klebsiella oxytoca in Europe
    S Brisse
    Eijkman Winkler Institute, Utrecht University, The Netherlands
    Eur J Clin Microbiol Infect Dis 19:64-8. 2000
    ..The newer fluoroquinolones sitafloxacin and clinafloxacin may become increasingly valuable, since they proved to be active also against ciprofloxacin-resistant isolates...
  71. pmc Invasive and noninvasive group A streptococcal isolates with different speA alleles in The Netherlands: genetic relatedness and production of pyrogenic exotoxins A and B
    E M Mascini
    Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, Utrecht University Hospital, Utrecht, The Netherlands
    J Clin Microbiol 37:3469-74. 1999
    ....
  72. ncbi [Fatal pneumonia in an adolescent due to community-acquired methicillin-resistant Staphylococcus aureus positive for Panton-Valentine-leukocidin]
    M van der Flier
    Wilhelmina Kinderziekenhuis, afd Algemene Intensive Care, Universitair Medisch Centrum Utrecht, Huispost KB 03 023 2, Postbus 85 090, 3508 AB Utrecht
    Ned Tijdschr Geneeskd 147:1076-9. 2003
    ..Especially in children and adolescents with an influenza virus infection, pneumonia due to PVL-positive S. aureus strains may be life-threatening...
  73. pmc Presence of integron-associated resistance in the community is widespread and contributes to multidrug resistance in the hospital
    M A Leverstein-Van Hall
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Center, Utrecht, The Netherlands
    J Clin Microbiol 40:3038-40. 2002
    ....
  74. pmc Novel mobile variants of staphylococcal cassette chromosome mec in Staphylococcus aureus
    W T M Jansen
    University Medical Center Utrecht, Eijkman Winkler Center for Medical Microbiology and Infectious Diseases, The Netherlands
    Antimicrob Agents Chemother 50:2072-8. 2006
    ..The relative abundance of and variety in SCCmec IV excisions may contribute to the frequency of horizontal transfer and genetic plasticity in SCCmec IV MRSA strains...
  75. pmc Chicken cathelicidins display antimicrobial activity against multiresistant bacteria without inducing strong resistance
    Edwin J A Veldhuizen
    Department of Infectious Diseases and Immunology, Division of Molecular Host Defence, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    PLoS ONE 8:e61964. 2013
    ..The combined results underline the potential of chicken cathelicidins as a new alternative to antibiotics...
  76. doi Shared reservoir of ccrB gene sequences between coagulase-negative staphylococci and methicillin-resistant Staphylococcus aureus
    Ad C Fluit
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    J Antimicrob Chemother 68:1707-13. 2013
    ..In this study, we determined the genetic relatedness of ccrB sequences contained in SCCmec elements of a spatio-temporally diverse and comprehensive collection of methicillin-resistant CoNS and S. aureus...
  77. pmc New methods to analyse microarray data that partially lack a reference signal
    Neeltje Carpaij
    Department of Medical Microbiology, University Medical Centre Utrecht, Room G04, 614 PO BOX 85500, 3508 GA Utrecht, The Netherlands
    BMC Genomics 10:522. 2009
    ..We aimed to develop novel methods to determine the presence or divergence of genes in a Staphylococcus aureus multistrain PCR product microarray-based CGH approach for which reference DNA was not available for some probes...
  78. pmc Yersiniabactin reduces the respiratory oxidative stress response of innate immune cells
    Armand Paauw
    Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands
    PLoS ONE 4:e8240. 2009
    ....
  79. ncbi [Identical resistance genes and plasmids in Escherichia coli from Dutch patients, poultry and retail chicken meat]
    Maurine A Leverstein-van Hall
    Universitair Medisch Centrum, Utrecht, Afd Medische Microbiologie, The Netherlands
    Ned Tijdschr Geneeskd 155:A3401. 2011
    ..coli isolates from patients in the Netherlands...
  80. pmc Epidemic and nonepidemic multidrug-resistant Enterococcus faecium
    Helen L Leavis
    University Medical Center Utrecht, Utrecht, The Netherlands
    Emerg Infect Dis 9:1108-15. 2003
    ..VREF and VSEF from different epidemiologic sources are genetically related; evidence exists for nosocomial selection of a subtype of E. faecium, which has acquired vancomycin-resistance through horizontal transfer...
  81. ncbi Comparison of E test and double disk diffusion test for detection of extended spectrum beta-lactamases
    A Florijn
    Eijkman Winkler Institute, University Medical Center Utrecht, PO Box 85500, Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 21:241-3. 2002
  82. pmc Whole Genome Analysis of Epidemiologically Closely Related Staphylococcus aureus Isolates
    Maarten Schijffelen
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 8:e78340. 2013
    ..The data indicate that differences between the isolate from infection and the colonizing isolate for S2395/S2396 and S2397/S2398 exist as well as between isolates from different hosts, but S1800/S1805 are not clonal. ..
  83. ncbi High levels of hydrolytic enzymes secreted by Candida albicans isolates involved in respiratory infections
    Annemarie Borst
    Eijkman Winkler Center for Microbiology, Infectious Diseases and Inflammation, University Medical Center, Utrecht, The Netherlands
    J Med Microbiol 52:971-4. 2003
    ..These virulent isolates involved in respiratory infections may originate from the oral cavity. Whether these results are caused by selection for these highly virulent isolates remains to be resolved...
  84. pmc Whole genome analysis of a livestock-associated methicillin-resistant Staphylococcus aureus ST398 isolate from a case of human endocarditis
    Maarten J Schijffelen
    Department of Medical Microbiology, University Medical Centre Utrecht, Heidelberglaan 100, 3508 GA, Utrecht, The Netherlands
    BMC Genomics 11:376. 2010
    ..aureus genomes in order to identify genetic traits that may explain the success of this particular lineage. Therefore, we determined the whole genome sequence of S0385, an MRSA ST398 isolate from a human case of endocarditis...
  85. ncbi Priorities for antibiotic resistance surveillance in Europe
    A C Fluit
    Eijkman Winkler Center for Medical Microbiology and Inflammation, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Clin Microbiol Infect 12:410-7. 2006
    ..As already noted by the WHO, genetic studies that include both the typing of isolates and the characterisation of resistance determinants are necessary to understand fully the spread and development of antibiotic resistance...
  86. pmc Activities of the glycylcycline tigecycline (GAR-936) against 1,924 recent European clinical bacterial isolates
    D Milatovic
    Eijkman Winkler Institute for Medical Microbiology, Utrecht, The Netherlands
    Antimicrob Agents Chemother 47:400-4. 2003
    ..Among the nonfermenters, Acinetobacter spp. and Stenotrophomonas maltophilia are included in the in vitro spectrum of tigecycline activity...
  87. pmc Novel gene cassettes and integrons
    E D Peters
    Eijkman-Winkler Institute, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
    Antimicrob Agents Chemother 45:2961-4. 2001
    ..Nine different integrons and 17 gene cassettes were found, including the new gene cassette aadA8. This cassette was highly related to aadA3 and aadA2. In addition, an unknown promoter sequence was found for two integrons...
  88. pmc International multicenter evaluation of the DiversiLab bacterial typing system for Escherichia coli and Klebsiella spp
    Guido M Voets
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Microbiol 51:3944-9. 2013
    ....
  89. pmc Use of amplified fragment length polymorphism analysis to identify medically important Candida spp., including C. dubliniensis
    A Borst
    Eijkman Winkler Center for Microbiology, Infectious Diseases and Inflammation, University Medical Center, Utrecht, The Netherlands
    J Clin Microbiol 41:1357-62. 2003
    ..AFLP analysis is universally applicable, and the patterns can easily be stored in a general, accessible database. Therefore, AFLP might prove to be a reliable method for the identification of medically important Candida species...
  90. pmc Monoclonal antibodies that react with live Listeria spp
    R Torensma
    Eijkman Winkler Laboratory for Medical Microbiology, University Hospital Utrecht, The Netherlands
    Appl Environ Microbiol 59:2713-6. 1993
    ..strains, one Escherichia coli strain, and one Citrobacter sp. strain. In Western blots (immunoblots) MAbs 55-23, 55-44, and 34-9 exhibited reactivity; all other MAbs were negative in this assay...
  91. pmc Comparison of genotypic and phenotypic methods for species-level identification of clinical isolates of coagulase-negative staphylococci
    E Heikens
    Eijkman Winkler Institute, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Microbiol 43:2286-90. 2005
    ..We propose partial sequencing of the tuf gene as a reliable and reproducible method for identification of CONS species...
  92. ncbi False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy
    A Borst
    Eijkman Winkler Center for Microbiology, Infectious Diseases and Inflammation, University Medical Center, PO Box 85500, 3508 GA Utrecht, The Netherlands
    Eur J Clin Microbiol Infect Dis 23:289-99. 2004
    ..The discussion of prevention and destruction strategies included in this review may serve as a guide to help improve laboratory practices and reduce the number of false-positive amplification results...
  93. ncbi In vitro activity of new ketolides against macrolide-susceptible and -resistant Staphylococcus aureus isolates with defined resistance gene status
    Franz Josef Schmitz
    J Antimicrob Chemother 49:580-2. 2002
  94. pmc In vitro activities of novel des-fluoro(6) quinolone BMS-284756 against mutants of Streptococcus pneumoniae, Streptococcus pyogenes, and Staphylococcus aureus selected with different quinolones
    Franz Josef Schmitz
    Antimicrob Agents Chemother 46:934-5. 2002
  95. pmc Genotyping of clinical methicillin-susceptible Staphylococcus aureus isolates in a Dutch teaching hospital
    Yvonne Van Dijk
    Department of Infection Control and Microbiology, Diakonessenhuis, Eijkman Winkler Institute for Microbiology, The Netherlands
    J Clin Microbiol 40:663-5. 2002
    ..Among the multiple S. aureus types six clonal lineages dominated, comprising isolates from 158 patients. Despite the limited genetic variation, cross-transmission was made plausible only sporadically...
  96. ncbi Occurrence and spread of SHV extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in Curacao
    Mireille van Westreenen
    J Antimicrob Chemother 52:530-2. 2003
  97. pmc Production of BRO beta-lactamases and resistance to complement in European Moraxella catarrhalis isolates
    Franz Josef Schmitz
    Institute for Medical Microbiology and Virology, Heinrich Heine Universitat Dusseldorf, Dusseldorf, Germany
    J Clin Microbiol 40:1546-8. 2002
    ..Nineteen percent of all isolates tested were complement sensitive. Resistance to beta-lactams is not linked to the phylogenetic lineages associated with susceptibility to complement...
  98. ncbi Characteristics of extended-spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates from horses
    An T T Vo
    Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, PO Box 80165, 3508 TD Utrecht, The Netherlands
    Vet Microbiol 124:248-55. 2007
    ..coli isolate have not yet been described before. To our knowledge this is the first report on ESBL's and AmpC beta-lactamases in equine E. coli and Klebsiella isolates...
  99. ncbi In vitro activity of faropenem against 5460 clinical bacterial isolates from Europe
    Dana Milatovic
    J Antimicrob Chemother 50:293-9. 2002
  100. ncbi Class 1 integrons in Enterobacteriaceae isolated from clinical infections of horses and dogs in the Netherlands
    Engeline van Duijkeren
    Veterinary Microbiological Diagnostic Center, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
    Microb Drug Resist 11:383-6. 2005
    ....
  101. ncbi Antimicrobial resistance among isolates cultured from patients hospitalized with lower respiratory tract infection in Europe
    Ad C Fluit
    Int J Infect Dis 6:144-6. 2002