Wilco de Jager

Summary

Affiliation: University Medical Center Utrecht
Country: The Netherlands

Publications

  1. pmc Prerequisites for cytokine measurements in clinical trials with multiplex immunoassays
    Wilco de Jager
    Department of Pediatric Immunology, Centre for Molecular and Cellular Intervention CMCI, University Medical Centre Utrecht, Utrecht, The Netherlands
    BMC Immunol 10:52. 2009
  2. doi request reprint Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis
    Wilco de Jager
    Department of Pediatrics, Centre for Cellular and Molecular Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Arthritis Rheum 60:2782-93. 2009
  3. doi request reprint Pan-DR-binding Hsp60 self epitopes induce an interleukin-10-mediated immune response in rheumatoid arthritis
    Huib de Jong
    University Medical Centre Utrecht, Utrecht, The Netherlands
    Arthritis Rheum 60:1966-76. 2009
  4. doi request reprint Hsp60 in inflamed muscle tissue is the target of regulatory autoreactive T cells in patients with juvenile dermatomyositis
    Elisabeth F Elst
    University Medical Center Utrecht, Utrecht, The Netherlands
    Arthritis Rheum 58:547-55. 2008
  5. ncbi request reprint Novel pan-DR-binding T cell epitopes of adenovirus induce pro-inflammatory cytokines and chemokines in healthy donors
    Lianne M Haveman
    Department of Pediatric Immunology and Hematology, Wilhelmina Children s Hospital, University Medical Center, KC 03 063 0, Lundlaan 6, Postbus 85090, 3508 AB, Utrecht, The Netherlands
    Int Immunol 18:1521-9. 2006
  6. pmc Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60)
    Joost A Aalberse
    Department of Pediatric Immunology, Wilhelmina Children s Hospital, Utrecht, The Netherlands
    PLoS ONE 6:e24119. 2011
  7. pmc Blood and synovial fluid cytokine signatures in patients with juvenile idiopathic arthritis: a cross-sectional study
    Wilco de Jager
    Department of Pediatric Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
    Ann Rheum Dis 66:589-98. 2007
  8. doi request reprint Vaccination leads to an aberrant FOXP3 T-cell response in non-remitting juvenile idiopathic arthritis
    Arash Ronaghy
    Department of Pediatrics, Center for Cellular and Molecular Intervention, Wilhelmina Children s Hospital, University Medical Center Utrecht, Utrecht, The Netherlands
    Ann Rheum Dis 70:2037-43. 2011
  9. doi request reprint Differential cytokine profiles in juvenile idiopathic arthritis subtypes revealed by cluster analysis
    Henk Jan van den Ham
    Theoretical Biology and Bioinformatics, Utrecht University, Utrecht, The Netherlands
    Rheumatology (Oxford) 48:899-905. 2009
  10. doi request reprint Bystander suppression of experimental arthritis by nasal administration of a heat shock protein peptide
    Evelien Zonneveld-Huijssoon
    Department of Pediatric Immunology, Centre for Molecular and Cellular Intervention, University Medical Centre Utrecht, The Netherlands
    Ann Rheum Dis 70:2199-206. 2011

Collaborators

Detail Information

Publications33

  1. pmc Prerequisites for cytokine measurements in clinical trials with multiplex immunoassays
    Wilco de Jager
    Department of Pediatric Immunology, Centre for Molecular and Cellular Intervention CMCI, University Medical Centre Utrecht, Utrecht, The Netherlands
    BMC Immunol 10:52. 2009
    ..Therefore we set out to investigate several processes that might impact cytokine profiles such as blood collecting tubes, duration of storage, and number of freeze thawing cycles...
  2. doi request reprint Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis
    Wilco de Jager
    Department of Pediatrics, Centre for Cellular and Molecular Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands
    Arthritis Rheum 60:2782-93. 2009
    ..Since IL-18 can drive NK cell function, we examined how the high plasma levels of this cytokine are related to the documented NK cell failure in these patients...
  3. doi request reprint Pan-DR-binding Hsp60 self epitopes induce an interleukin-10-mediated immune response in rheumatoid arthritis
    Huib de Jong
    University Medical Centre Utrecht, Utrecht, The Netherlands
    Arthritis Rheum 60:1966-76. 2009
    ..This study was undertaken to characterize the disease specificity and function of pan-DR-binding Hsp60-derived epitopes as possible modulators of autoimmune inflammation in RA...
  4. doi request reprint Hsp60 in inflamed muscle tissue is the target of regulatory autoreactive T cells in patients with juvenile dermatomyositis
    Elisabeth F Elst
    University Medical Center Utrecht, Utrecht, The Netherlands
    Arthritis Rheum 58:547-55. 2008
    ..The purpose of this study was to determine whether Hsp60 could serve as an autoantigen in juvenile DM...
  5. ncbi request reprint Novel pan-DR-binding T cell epitopes of adenovirus induce pro-inflammatory cytokines and chemokines in healthy donors
    Lianne M Haveman
    Department of Pediatric Immunology and Hematology, Wilhelmina Children s Hospital, University Medical Center, KC 03 063 0, Lundlaan 6, Postbus 85090, 3508 AB, Utrecht, The Netherlands
    Int Immunol 18:1521-9. 2006
    ..Based on the broad recognition and the induced cytokine and chemokine profile, the detected epitopes can be regarded as potential candidates to select adenovirus-specific T cells for immune intervention in the immunocompromised host...
  6. pmc Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60)
    Joost A Aalberse
    Department of Pediatric Immunology, Wilhelmina Children s Hospital, Utrecht, The Netherlands
    PLoS ONE 6:e24119. 2011
    ..It is not known whether HSP60-specific T cells are present at birth and thus may play a role in neonatal tolerance. We studied whether self-HSP60 reactive T cells are present in cord blood, and if so, what phenotype these cells have...
  7. pmc Blood and synovial fluid cytokine signatures in patients with juvenile idiopathic arthritis: a cross-sectional study
    Wilco de Jager
    Department of Pediatric Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
    Ann Rheum Dis 66:589-98. 2007
    ..Although onset and disease course differ, the subtypes of JIA share the occurrence of chronic inflammation of the joints, with infiltrations of immunocompetent cells that secrete inflammatory mediators...
  8. doi request reprint Vaccination leads to an aberrant FOXP3 T-cell response in non-remitting juvenile idiopathic arthritis
    Arash Ronaghy
    Department of Pediatrics, Center for Cellular and Molecular Intervention, Wilhelmina Children s Hospital, University Medical Center Utrecht, Utrecht, The Netherlands
    Ann Rheum Dis 70:2037-43. 2011
    ....
  9. doi request reprint Differential cytokine profiles in juvenile idiopathic arthritis subtypes revealed by cluster analysis
    Henk Jan van den Ham
    Theoretical Biology and Bioinformatics, Utrecht University, Utrecht, The Netherlands
    Rheumatology (Oxford) 48:899-905. 2009
    ..In particular, we focus on juvenile idiopathic arthritis (JIA), a multifactorial autoimmune disorder that ultimately leads to chronic inflammation of the joints...
  10. doi request reprint Bystander suppression of experimental arthritis by nasal administration of a heat shock protein peptide
    Evelien Zonneveld-Huijssoon
    Department of Pediatric Immunology, Centre for Molecular and Cellular Intervention, University Medical Centre Utrecht, The Netherlands
    Ann Rheum Dis 70:2199-206. 2011
    ..A study was undertaken to determine whether mucosal administration of these bystander epitopes can be similarly effective in suppressing arthritis...
  11. doi request reprint Selection of perforin expressing CD4+ adenovirus-specific T-cells with artificial antigen presenting cells
    Lianne M Haveman
    Department of Paediatric Immunology and Haematology, University Medical Centre Utrecht, Wilhelmina Children s Hospital Utrecht, The Netherlands
    Clin Immunol 146:228-39. 2013
    ..Thus, the generation and isolation of HAdV-specific CD4+ T-cells seem a critical step towards specific adoptive therapy for HAdV infections after allogeneic SCT...
  12. ncbi request reprint TLR9 agonist CpG enhances protective nasal HSP60 peptide vaccine efficacy in experimental autoimmune arthritis
    Evelien Zonneveld-Huijssoon
    Department of Pediatric Immunology, University Medical Centre Utrecht, Centre for Molecular and Cellular Intervention, Lundlaan 6, 3584 EA Utrecht, The Netherlands
    Ann Rheum Dis 71:1706-15. 2012
    ..The authors focused on the Toll-like receptor 9 ligand CpG, which was shown to stimulate regulatory T-cell proliferation when added to plasmacytoid dendritic cells (pDC) using in-vitro cultures...
  13. pmc Simultaneous detection of 15 human cytokines in a single sample of stimulated peripheral blood mononuclear cells
    Wilco de Jager
    Department of Pediatric Immunology, University Medical Center Utrecht, Wilhelmina Children s Hospital, 3584 EA Utrecht, The Netherlands
    Clin Diagn Lab Immunol 10:133-9. 2003
    ..It will provide a more complete picture in differences between activated lymphocyte cytokine profiles from healthy individuals and those from patients with chronic inflammatory diseases...
  14. ncbi request reprint Improved multiplex immunoassay performance in human plasma and synovial fluid following removal of interfering heterophilic antibodies
    Wilco de Jager
    Department of Pediatric Immunology, University Medical Center Utrecht, Wilhelmina Children s Hospital, Utrecht, The Netherlands
    J Immunol Methods 300:124-35. 2005
    ..We show that pre-absorption with protein-L is a powerful tool for removal of interfering immunoglobulins from human bodily fluids to be used in immunoassays for studying changes in protein patterns...
  15. pmc Protein expression profiling of inflammatory mediators in human temporal lobe epilepsy reveals co-activation of multiple chemokines and cytokines
    Anne A Kan
    Department of Neuroscience and Pharmacology, Rudolf Magnus Institute of Neuroscience, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    J Neuroinflammation 9:207. 2012
    ..Our data shows co-activation of multiple inflammatory mediators in hippocampus and neocortex of mTLE patients, indicating activation of multiple pro- and anti-epileptogenic immune pathways in this disease...
  16. ncbi request reprint Solid-phase and bead-based cytokine immunoassay: a comparison
    Wilco de Jager
    Department of Pediatric Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
    Methods 38:294-303. 2006
    ..Due to an increased interest in the integral approach to understand biological processes (the omics era), multiplex immunoassays for detection of cytokines and the interpretation of these assays are gaining popularity...
  17. doi request reprint Cytokine assays: an assessment of the preparation and treatment of blood and tissue samples
    Genoveva C E Keustermans
    Department of Pediatric Immunology KC01 069 0, Centre for Molecular and Cellular Intervention, University Medical Centre Utrecht, Utrecht, The Netherlands
    Methods 61:10-7. 2013
    ..By having a clear understanding of what factors influence cytokine levels and how to overcome these technical issues, minimally confounded data can be obtained and cytokines can achieve optimal biomarker activity...
  18. pmc Recognition of self-heat shock protein 60 by T cells from patients with atopic dermatitis
    Berber Kapitein
    Department of General Paediatrics, Wilhelmina Children s Hospital, Utrecht, The Netherlands
    Cell Stress Chaperones 18:87-95. 2013
    ..hsp60 is recognized by proinflammatory (IFNγ high, IL-10 low) T cells in atopic patients and is more present in lesional AD skin. This suggests that hsp60-specific T cell responses contribute to local inflammation in AD...
  19. pmc Human regulatory T cell suppressive function is independent of apoptosis induction in activated effector T cells
    Yvonne Vercoulen
    Department of Pediatric Immunology, Center for Molecular and Cellular Intervention, Wilhelmina Children s Hospital, University Medical Center Utrecht, Utrecht, The Netherlands
    PLoS ONE 4:e7183. 2009
    ..Experiments in mice demonstrated that Treg restrict effector T cell (Teff) responses by deprivation of the growth factor IL-2 through Treg consumption, resulting in apoptosis of Teff...
  20. doi request reprint Cytokine multiplex immunoassay: methodology and (clinical) applications
    Wilco de Jager
    Department of Pediatric Immunology, Wilhelmina Children s Hospital, University Medical Center Utrecht, Utrecht, The Netherlands
    Methods Mol Biol 514:119-33. 2009
    ..The power and robustness of the MIA technique is illustrated by an analysis of cytokine profiles in juvenile idiopathic arthritis...
  21. doi request reprint Different cytokine signatures in children with localized and invasive adenovirus infection after stem cell transplantation
    Lianne M Haveman
    Department of Paediatric Immunology and Haematology, University Medical Centre Utrecht, Wilhelmina Children s Hospital Utrecht, Utrecht, The Netherlands
    Pediatr Transplant 14:520-8. 2010
    ..In conclusion, after SCT, children with invasive HAdV infection have a different cytokine profile compared with patients with a localized HAdV infection...
  22. ncbi request reprint Brief report: Autologous stem cell transplantation restores immune tolerance in experimental arthritis by renewal and modulation of the Teff cell compartment
    Eveline M Delemarre
    University Medical Center Utrecht and Wilhelmina Children s Hospital, Utrecht, The Netherlands
    Arthritis Rheumatol 66:350-6. 2014
    ..This study was undertaken to further unravel the immunologic mechanisms underlying ASCT by using a mouse model of proteoglycan-induced arthritis (PGIA)...
  23. ncbi request reprint Tolerogenic immune responses to novel T-cell epitopes from heat-shock protein 60 in juvenile idiopathic arthritis
    Sylvia Kamphuis
    Department of Paediatric Immunology, IACOPO Institute for Translational Medicine, University Medical Centre Utrecht, Utrecht, The Netherlands
    Lancet 366:50-6. 2005
    ..We sought to identify HSP60 T-cell epitopes to find potential targets for HSP60 immunotherapy and to assess whether immune responses to these epitopes contribute to the distinct clinical outcome of this disease...
  24. ncbi request reprint Effectiveness of first-line treatment with recombinant interleukin-1 receptor antagonist in steroid-naive patients with new-onset systemic juvenile idiopathic arthritis: results of a prospective cohort study
    Sebastiaan J Vastert
    University Medical Centre Utrecht, Utrecht, The Netherlands
    Arthritis Rheumatol 66:1034-43. 2014
    ..To conduct a prospective cohort study using anakinra, a recombinant IL-1 receptor antagonist (IL-1Ra), as first-line therapy in patients with new-onset systemic juvenile idiopathic arthritis (JIA)...
  25. ncbi request reprint T cell recognition of naturally presented epitopes of self-heat shock protein 70
    Huib de Jong
    Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
    Cell Stress Chaperones 19:569-78. 2014
    ..The identification of these naturally presented epitopes and the response they elicit in the normal immune system make them potential candidates to study during inflammatory conditions as well as in autoimmune diseases. ..
  26. doi request reprint Survival, but not maturation, is affected in neutrophil progenitors from GSD-1b patients
    Gepke Visser
    Department of Metabolic Diseases, Wilhelmina Children s Hospital, University Medical Center Utrecht, Utrecht, The Netherlands
    J Inherit Metab Dis 35:287-300. 2012
    ....
  27. doi request reprint Functional human regulatory T cells fail to control autoimmune inflammation due to PKB/c-akt hyperactivation in effector cells
    Ellen J Wehrens
    Center for Molecular and Cellular Intervention, Department of Immunology, Wilhelmina Children s Hospital, University Medical Center Utrecht, Utrecht, The Netherlands
    Blood 118:3538-48. 2011
    ..Furthermore, these findings suggest that for a Treg enhancing strategy to be successful in the treatment of autoimmune inflammation, resistance because of PKB/c-akt hyperactivation should be targeted as well...
  28. doi request reprint Mutations in the perforin gene can be linked to macrophage activation syndrome in patients with systemic onset juvenile idiopathic arthritis
    Sebastiaan J Vastert
    Department of Paediatric Immunology, Wilhelmina Children s Hospital, University Medical Centre Utrecht, Lundlaan 6, 3584 EA Utrecht, The Netherlands
    Rheumatology (Oxford) 49:441-9. 2010
    ..Interestingly, SoJIA patients display decreased levels of perforin in NK cells and diminished NK cell function as well. Here, we analysed PRF1 and its putative promoter in SoJIA patients with or without a history of MAS...
  29. ncbi request reprint CD4+CD25bright regulatory T cells actively regulate inflammation in the joints of patients with the remitting form of juvenile idiopathic arthritis
    Ismé M de Kleer
    University Medical Center Utrecht, Wilhelmina Children s Hospital, Department of Pediatric Immunology and Immunology Advanced Center on Preclinical Immuno genomics Institute for Translational Medicine, Utrecht, The Netherlands
    J Immunol 172:6435-43. 2004
    ..These data suggest that CD4(+)CD25(bright) Treg cells play a role in determining the patient's fate toward either a favorable or unfavorable clinical course of disease...
  30. doi request reprint Low-grade adipose tissue inflammation in patients with mild-to-moderate chronic obstructive pulmonary disease
    Bram van den Borst
    NUTRIM School for Nutrition, Toxicology and Metabolism, Department of Respiratory Medicine, Maastricht University Medical Center, Netherlands
    Am J Clin Nutr 94:1504-12. 2011
    ..Adipose tissue is a potent producer of inflammatory mediators and may contribute to systemic inflammation in COPD, possibly via hypoxia...
  31. pmc Genome-wide microRNA profiling of human temporal lobe epilepsy identifies modulators of the immune response
    Anne A Kan
    Department of Neuroscience and Pharmacology, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands
    Cell Mol Life Sci 69:3127-45. 2012
    ..Overall, this work extends the current concepts of human mTLE pathogenesis to the level of miRNA-mediated gene regulation...
  32. doi request reprint Autologous bone marrow transplantation in autoimmune arthritis restores immune homeostasis through CD4+CD25+Foxp3+ regulatory T cells
    Sarah T A Roord
    Department of Pediatric Immunology, University Medical Center Utrecht, Wilhelmina Children s Hospital, Utrecht, USA
    Blood 111:5233-41. 2008
    ..This work provided further insight into the role of Tregs in restoration of the immune balance by aBMT and can open the way to explore therapeutic interventions to further improve treatment of AID and disease relapses...