Christian Blum

Summary

Affiliation: University of Twente
Country: The Netherlands

Publications

  1. ncbi request reprint Nanophotonic control of the Förster resonance energy transfer efficiency
    Christian Blum
    Nanobiophysics NBP, MESA Institute for Nanotechnology, University of Twente, 7500 AE Enschede, The Netherlands
    Phys Rev Lett 109:203601. 2012
  2. ncbi request reprint Single oligomer spectra probe chromophore nanoenvironments of tetrameric fluorescent proteins
    Christian Blum
    Biophysical Engineering Group, MESA Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    J Am Chem Soc 128:8664-70. 2006
  3. doi request reprint Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging
    Denis Dorokhin
    Materials Science and Technology of Polymers, Faculty of Science and Technology and Mesa Institute for Nanotechnology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    Small 6:2870-6. 2010
  4. doi request reprint Manipulation of the local density of photonic states to elucidate fluorescent protein emission rates
    Yanina Cesa
    Biophysical Engineering Group, Faculty of Science and Technology and Mesa Institute for Nanotechnology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    Phys Chem Chem Phys 11:2525-31. 2009
  5. doi request reprint Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels
    Yujie Ma
    Nanobiophysics, MESA Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands
    J Colloid Interface Sci 356:123-30. 2011
  6. doi request reprint Dark proteins disturb multichromophore coupling in tetrameric fluorescent proteins
    Christian Blum
    Nanobiophysics, MESA Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    J Biophotonics 4:114-21. 2011
  7. doi request reprint Color control of natural fluorescent proteins by photonic crystals
    Christian Blum
    Biophysical Engineering Group, Faculty of Science and Technology, MESA Institute for Nanotechnology, University of Twente, 7500 AE Enschede, The Netherlands
    Small 4:492-6. 2008
  8. doi request reprint Non-invasive imaging through opaque scattering layers
    Jacopo Bertolotti
    Complex Photonic Systems, MESA Institute for Nanotechnology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands
    Nature 491:232-4. 2012
  9. doi request reprint Temperature-modulated quenching of quantum dots covalently coupled to chain ends of poly(N-isopropyl acrylamide) brushes on gold
    Oya Tagit
    Materials Science and Technology of Polymers, Faculty of Science and Technology and Mesa Institute for Nanotechnology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands
    Nanotechnology 20:185501. 2009
  10. doi request reprint Single-molecule spectroscopy of fluorescent proteins
    Christian Blum
    Biophysical Engineering Group, MESA Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, P O Box 217, 7500, AE Enschede, The Netherlands
    Anal Bioanal Chem 393:527-41. 2009

Collaborators

Detail Information

Publications20

  1. ncbi request reprint Nanophotonic control of the Förster resonance energy transfer efficiency
    Christian Blum
    Nanobiophysics NBP, MESA Institute for Nanotechnology, University of Twente, 7500 AE Enschede, The Netherlands
    Phys Rev Lett 109:203601. 2012
    ..Our results imply a change in the characteristic Förster distance, in contrast to common lore that this distance is fixed for a given FRET pair...
  2. ncbi request reprint Single oligomer spectra probe chromophore nanoenvironments of tetrameric fluorescent proteins
    Christian Blum
    Biophysical Engineering Group, MESA Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    J Am Chem Soc 128:8664-70. 2006
    ....
  3. doi request reprint Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging
    Denis Dorokhin
    Materials Science and Technology of Polymers, Faculty of Science and Technology and Mesa Institute for Nanotechnology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    Small 6:2870-6. 2010
    ....
  4. doi request reprint Manipulation of the local density of photonic states to elucidate fluorescent protein emission rates
    Yanina Cesa
    Biophysical Engineering Group, Faculty of Science and Technology and Mesa Institute for Nanotechnology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    Phys Chem Chem Phys 11:2525-31. 2009
    ..For EGFP we determine the quantum efficiency of the on-states to be 72%. As expected for this complex system, our value is higher than that determined by methods that average over on- and off-states...
  5. doi request reprint Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels
    Yujie Ma
    Nanobiophysics, MESA Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands
    J Colloid Interface Sci 356:123-30. 2011
    ..This inorganic-protein host-guest system is an example of a nanobiophotonic hybrid system that may lead to composite materials with novel optical properties...
  6. doi request reprint Dark proteins disturb multichromophore coupling in tetrameric fluorescent proteins
    Christian Blum
    Nanobiophysics, MESA Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    J Biophotonics 4:114-21. 2011
    ..We show that these species lead to donor dequenching that strongly influences the bulk emission spectra...
  7. doi request reprint Color control of natural fluorescent proteins by photonic crystals
    Christian Blum
    Biophysical Engineering Group, Faculty of Science and Technology, MESA Institute for Nanotechnology, University of Twente, 7500 AE Enschede, The Netherlands
    Small 4:492-6. 2008
    ..This "biophotonic engineering" may be extended to control emission rates and complex Förster energy-transfer systems obtained by protein engineering...
  8. doi request reprint Non-invasive imaging through opaque scattering layers
    Jacopo Bertolotti
    Complex Photonic Systems, MESA Institute for Nanotechnology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands
    Nature 491:232-4. 2012
    ..This approach to non-invasive imaging through strongly scattering media can be generalized to other contrast mechanisms and geometries...
  9. doi request reprint Temperature-modulated quenching of quantum dots covalently coupled to chain ends of poly(N-isopropyl acrylamide) brushes on gold
    Oya Tagit
    Materials Science and Technology of Polymers, Faculty of Science and Technology and Mesa Institute for Nanotechnology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands
    Nanotechnology 20:185501. 2009
    ..The data obtained are consistent with temperature-modulated thickness changes of the PNIPAM layer and quenching of the QDs by the gold surface via nonradiative energy transfer...
  10. doi request reprint Single-molecule spectroscopy of fluorescent proteins
    Christian Blum
    Biophysical Engineering Group, MESA Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, P O Box 217, 7500, AE Enschede, The Netherlands
    Anal Bioanal Chem 393:527-41. 2009
    ..In this paper we review how single-molecule emission detection has helped understanding of the complex photophysics of fluorescent proteins...
  11. ncbi request reprint Molecular Composition of Sub-stoichiometrically Labeled α-Synuclein Oligomers Determined by Single-Molecule Photobleaching
    Niels Zijlstra
    Nanobiophysics, MESA Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P O Box 217, 7500 AE Enschede The Netherlands
    Angew Chem Int Ed Engl 51:8821-4. 2012
    ..The results show that this α-synuclein oligomer is present as a single, well-defined species consisting of 31 monomers...
  12. doi request reprint FRET pair printing of fluorescent proteins
    Maryana Escalante
    Biophysical Engineering Group, MESA Institute for Nanotechnology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    Langmuir 25:7019-24. 2009
    ..We quantitatively visualized the energy transfer by multiparameter optical microscopy...
  13. pmc Room temperature excitation spectroscopy of single quantum dots
    Christian Blum
    Nanobiophysics Group and MESA Institute for Nanotechnology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands
    Beilstein J Nanotechnol 2:516-24. 2011
    ..We expect that the additional capability of recording excitation spectra at room temperature from single emitters will enable insights into the photophysics of emitters that so far have remained inaccessible...
  14. doi request reprint Spectral versatility of single reef coral fluorescent proteins detected by spectrally-resolved single molecule spectroscopy
    Christian Blum
    Biophysical Engineering Group and MESA Institute for Nanotechnology and Institute for Biomedical Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands
    Chemphyschem 9:310-5. 2008
    ..Our results have implications for the accurate interpretation of biological and biochemical processes illuminated by fluorescent proteins as well as for choosing appropriate experimental configurations...
  15. doi request reprint Expression of sensitized Eu(3+) luminescence at a multivalent interface
    Shu Han Hsu
    Molecular Nanofabrication Group, MESA Institute for Nanotechnology, University of Twente, P O Box 217, 7500 AE Enschede, The Netherlands
    J Am Chem Soc 131:12567-9. 2009
    ..The system constitutes therefore an example of supramolecular expression of a complex consisting of several different building blocks which signals its own correct formation...
  16. doi request reprint New insights into the photophysics of DsRed by multiparameter spectroscopy on single proteins
    Frank Schleifenbaum
    Institute of Physical and Theoretical Chemistry, University of Tuebingen, Tuebingen, Germany
    J Phys Chem B 112:7669-74. 2008
    ..Hence in combination with X-ray studies our data allow for an accurate quantitative description of the radiative and nonradiative relaxation processes in DsRed proteins...
  17. doi request reprint Time, space, and spectrally resolved studies on J-aggregate interactions in zeolite L nanochannels
    Michael Busby
    Physikalisches Institut and Center for Nanotechnology, CeNTech, University of Munster, Mendelstrasse 7, 48149 Munster, Germany
    J Am Chem Soc 130:10970-6. 2008
    ..The new band is assigned to an inline arrangement of the molecules undergoing a J-aggregate-type coupling, a process so far not observed in subnanometer channels...
  18. ncbi request reprint Correlation of emission intensity and spectral diffusion in room temperature single-molecule spectroscopy
    Frank Stracke
    Fraunhofer Institute for Biomedical Engineering, Ensheimer Str 48, 66386 St Ingbert, Germany
    Chemphyschem 6:1242-6. 2005
  19. pmc Room temperature spectrally resolved single-molecule spectroscopy reveals new spectral forms and photophysical versatility of aequorea green fluorescent protein variants
    Christian Blum
    Physical Chemistry I, University of Siegen, Siegen, Germany
    Biophys J 87:4172-9. 2004
    ....