P Borst

Summary

Affiliation: The Netherlands Cancer Institute
Country: The Netherlands

Publications

  1. ncbi request reprint Ethidium DNA agarose gel electrophoresis: how it started
    Piet Borst
    Division of Molecular Biology, Centre of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    IUBMB Life 57:745-7. 2005
  2. ncbi request reprint Multidrug resistance-associated proteins 3, 4, and 5
    Piet Borst
    Division of Molecular Biology and Center of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Pflugers Arch 453:661-73. 2007
  3. pmc Telomeric localization of the modified DNA base J in the genome of the protozoan parasite Leishmania
    Paul André Genest
    Division of Molecular Biology and Centre of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Nucleic Acids Res 35:2116-24. 2007
  4. pmc The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase
    Zhong Yu
    Division of Molecular Biology and Centre of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Nucleic Acids Res 35:2107-15. 2007
  5. pmc Formation of linear inverted repeat amplicons following targeting of an essential gene in Leishmania
    Paul André Genest
    Division of Molecular Biology and Centre of Biomedical Genetics, The Netherlands Cancer Institute Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nucleic Acids Res 33:1699-709. 2005
  6. pmc Cancer drug pan-resistance: pumps, cancer stem cells, quiescence, epithelial to mesenchymal transition, blocked cell death pathways, persisters or what?
    Piet Borst
    Molecular Oncology, NKI AVL, Plesmanlaan 121, Amsterdam, The Netherlands
    Open Biol 2:120066. 2012
  7. ncbi request reprint Antigenic variation and allelic exclusion
    Piet Borst
    The Netherlands Cancer Institute, Division of Molecular Biology and Center for Biomedical Genetics, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Cell 109:5-8. 2002
  8. ncbi request reprint Mammalian ABC transporters in health and disease
    P Borst
    Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Annu Rev Biochem 71:537-92. 2002
  9. ncbi request reprint Do predictive signatures really predict response to cancer chemotherapy?
    Piet Borst
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam, The Netherlands
    Cell Cycle 9:4836-40. 2010
  10. ncbi request reprint How do real tumors become resistant to cisplatin?
    Piet Borst
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam, The Netherlands
    Cell Cycle 7:1353-9. 2008

Detail Information

Publications110 found, 100 shown here

  1. ncbi request reprint Ethidium DNA agarose gel electrophoresis: how it started
    Piet Borst
    Division of Molecular Biology, Centre of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    IUBMB Life 57:745-7. 2005
    ..We concluded that the linear DNAs 'crawl like snakes head on through the gel'. This paper reviews some of the early experiments preceding the introduction of ethidium agarose gel electrophoresis...
  2. ncbi request reprint Multidrug resistance-associated proteins 3, 4, and 5
    Piet Borst
    Division of Molecular Biology and Center of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Pflugers Arch 453:661-73. 2007
    ..The physiological function of MRP4 and 5 remains to be found...
  3. pmc Telomeric localization of the modified DNA base J in the genome of the protozoan parasite Leishmania
    Paul André Genest
    Division of Molecular Biology and Centre of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Nucleic Acids Res 35:2116-24. 2007
    ..brucei, Trypanosoma equiperdum and Trypanoplasma borreli. Our results suggest that J is a telomeric base modification, recruited for other (unknown) functions in some kinetoplastids and Euglena...
  4. pmc The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase
    Zhong Yu
    Division of Molecular Biology and Centre of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Nucleic Acids Res 35:2107-15. 2007
    ..We propose that JBP1 is a thymidine hydroxylase responsible for the local amplification of J inserted by JBP2, another putative thymidine hydroxylase...
  5. pmc Formation of linear inverted repeat amplicons following targeting of an essential gene in Leishmania
    Paul André Genest
    Division of Molecular Biology and Centre of Biomedical Genetics, The Netherlands Cancer Institute Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nucleic Acids Res 33:1699-709. 2005
    ..We propose that these amplicons have arisen by a template switch inside a DNA replication fork involving the inverted DNA repeats and helped by the gene targeting...
  6. pmc Cancer drug pan-resistance: pumps, cancer stem cells, quiescence, epithelial to mesenchymal transition, blocked cell death pathways, persisters or what?
    Piet Borst
    Molecular Oncology, NKI AVL, Plesmanlaan 121, Amsterdam, The Netherlands
    Open Biol 2:120066. 2012
    ..My conclusion is that it is time for a major effort to solve this mystery using the new genetically modified mouse tumour models that produce real tumours resembling cancer in human patients...
  7. ncbi request reprint Antigenic variation and allelic exclusion
    Piet Borst
    The Netherlands Cancer Institute, Division of Molecular Biology and Center for Biomedical Genetics, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Cell 109:5-8. 2002
    ..The possibility that such a structure might explain monoallelic expression in other multigene systems is discussed here...
  8. ncbi request reprint Mammalian ABC transporters in health and disease
    P Borst
    Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Annu Rev Biochem 71:537-92. 2002
    ..c) A rapidly increasing number of ABC transporters are found to play a role in lipid transport. Defects in each of these transporters are involved in human inborn or acquired diseases...
  9. ncbi request reprint Do predictive signatures really predict response to cancer chemotherapy?
    Piet Borst
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam, The Netherlands
    Cell Cycle 9:4836-40. 2010
    ..We delineate alternative approaches that should be able to yield predictive markers that can be used for optimizing patient treatment...
  10. ncbi request reprint How do real tumors become resistant to cisplatin?
    Piet Borst
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam, The Netherlands
    Cell Cycle 7:1353-9. 2008
    ..Taken together with the mouse mammary tumor data, these observations raise the possibility that proliferating cells have no readily available mechanism to escape from cisplatin DNA damage once their HR is irreversibly inactivated...
  11. ncbi request reprint What makes tumors multidrug resistant?
    Piet Borst
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam, The Netherlands
    Cell Cycle 6:2782-7. 2007
    ....
  12. ncbi request reprint Bill Slater at 90
    Piet Borst
    The Netherlands Cancer Institute Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    IUBMB Life 59:48-9. 2007
  13. doi request reprint Base J: discovery, biosynthesis, and possible functions
    Piet Borst
    Center of Biomedical Genetics, Division of Molecular Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Annu Rev Microbiol 62:235-51. 2008
    ....
  14. ncbi request reprint MRP2 and 3 in health and disease
    P Borst
    Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cancer Lett 234:51-61. 2006
    ..Instead, we have found a role for MRP3 in the cellular export of drug-glucuronide conjugates. We discuss problems in extrapolating results obtained for murine MRPs...
  15. ncbi request reprint The potential impact of drug transporters on nucleoside-analog-based antiviral chemotherapy
    P Borst
    Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Antiviral Res 62:1-7. 2004
    ..We briefly review how ABC transporters in general, and MRPs in particular, could affect the disposition and cellular accumulation of antiviral compounds...
  16. ncbi request reprint Does the absence of ABCC6 (multidrug resistance protein 6) in patients with Pseudoxanthoma elasticum prevent the liver from providing sufficient vitamin K to the periphery?
    Piet Borst
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam, The Netherlands
    Cell Cycle 7:1575-9. 2008
    ..The hypothesis implies that the symptoms of PXE can be prevented or mitigated by providing patients (intravenously) with a form of plasma vitamin K (precursor) that can be used by peripheral tissues...
  17. ncbi request reprint From cancer cells to trypanosomes and back again
    P Borst
    Division of Molecular Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam
    Cell Mol Life Sci 63:745-54. 2006
  18. ncbi request reprint How I became a biochemist
    Piet Borst
    Netherlands Cancer Institute, Clinical Biochemistry at the University of Amsterdam, The Netherlands
    IUBMB Life 58:177-82. 2006
  19. ncbi request reprint On the putative co-transport of drugs by multidrug resistance proteins
    P Borst
    Center of Biomedical Genetics, Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    FEBS Lett 580:1085-93. 2006
    ..We conclude that there is no unambiguous proof for co-transport of two different ligands by MRPs, but that cross-stimulated transport can explain the published data...
  20. pmc Altered MRP is associated with multidrug resistance and reduced drug accumulation in human SW-1573 cells
    E W Eijdems
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    Br J Cancer 72:298-306. 1995
    ..This mechanism can be accompanied by other resistance mechanisms, such as reduced topo II alpha mRNA in case of doxorubicin selection...
  21. ncbi request reprint Transport of the glutathione conjugate of ethacrynic acid by the human multidrug resistance protein MRP
    G J Zaman
    Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam, The Netherlands
    FEBS Lett 391:126-30. 1996
    ..This implies that ethacrynic acid may modulate drug resistance of tumor cells not only by inhibiting glutathione S-transferase activity, but also by inhibiting the export of drug conjugates from the cell by MRP...
  22. ncbi request reprint ABC transporters in lipid transport
    P Borst
    Division of Molecular Biology and Centre for Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam
    Biochim Biophys Acta 1486:128-44. 2000
    ..A third example, the ABC transporter involved in the import of long-chain fatty acids into peroxisomes, is discussed in the chapter by Hettema and Tabak in this volume...
  23. ncbi request reprint Increased sensitivity to anticancer drugs and decreased inflammatory response in mice lacking the multidrug resistance-associated protein
    J Wijnholds
    Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam
    Nat Med 3:1275-9. 1997
    ..Our results suggest that this ubiquitous GS-X pump is dispensable in mice, making treatment of MDR with MRP-specific reversal agents potentially feasible...
  24. ncbi request reprint Characterization of the promoter region of the human MDR3 P-glycoprotein gene
    J J Smit
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam
    Biochim Biophys Acta 1261:44-56. 1995
    ..The promoter region contains several consensus sequences where known or putative liver-specific (C/EBP, HNF5) or lymphoid specific (Pu.1, ets-1) transcription factors may bind...
  25. ncbi request reprint The multidrug resistance protein family
    P Borst
    Division of Molecular Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, Netherlands
    Biochim Biophys Acta 1461:347-57. 1999
    ....
  26. ncbi request reprint Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines
    M Kool
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    Cancer Res 57:3537-47. 1997
    ..Our results emphasize the need for gene-specific blocks in gene expression to define which transporter contributes to resistance in each resistant cell line...
  27. pmc MRP3, an organic anion transporter able to transport anti-cancer drugs
    M Kool
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 96:6914-9. 1999
    ..We discuss the possible function of MRP3 in (hepatic) physiology and its potential contribution to drug resistance of cancer cells...
  28. pmc Inhibitory effect of the reversal agents V-104, GF120918 and Pluronic L61 on MDR1 Pgp-, MRP1- and MRP2-mediated transport
    R Evers
    The Netherlands Cancer Institute, Division of Molecular Biology and Center for Biomedical Genetics, Amsterdam
    Br J Cancer 83:366-74. 2000
    ..Unexpectedly, export of the organic anion calcein by MDCKII-MRP1 and MDCKII-MRP2 cells is stimulated by Pluronic L61, probably because it relieves the block on entry of calcein AM into the cell by endogenous MDR1 Pgp...
  29. pmc Drug export activity of the human canalicular multispecific organic anion transporter in polarized kidney MDCK cells expressing cMOAT (MRP2) cDNA
    R Evers
    Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Clin Invest 101:1310-9. 1998
    ..We conclude that cMOAT is a 5'-adenosine triphosphate binding cassette transporter that potentially might be involved in drug resistance in mammalian cells...
  30. pmc Multidrug resistance protein 1 protects the choroid plexus epithelium and contributes to the blood-cerebrospinal fluid barrier
    J Wijnholds
    Division of Molecular Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    J Clin Invest 105:279-85. 2000
    ..Our results indicate that Mrp1 helps to limit tissue distribution of certain drugs and contributes to the blood-CSF drug-permeability barrier...
  31. pmc Multidrug-resistance protein 5 is a multispecific organic anion transporter able to transport nucleotide analogs
    J Wijnholds
    Division of Molecular Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 97:7476-81. 2000
    ..We speculate that MRP5 might play a role in some cases of unexplained resistance to thiopurines in acute lymphoblastic leukemia and/or to antiretroviral nucleoside analogs in HIV-infected patients...
  32. pmc Normal viability and altered pharmacokinetics in mice lacking mdr1-type (drug-transporting) P-glycoproteins
    A H Schinkel
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    Proc Natl Acad Sci U S A 94:4028-33. 1997
    ..mdr1a/1b (-/-) mice should provide a useful model system to further test the pharmacological roles of the drug-transporting P-gps and to analyze the specificity and effectivity of P-gp-blocking drugs...
  33. pmc The modified base J is the target for a novel DNA-binding protein in kinetoplastid protozoans
    M Cross
    Division of Molecular Biology and Centre of Biomedical Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    EMBO J 18:6573-81. 1999
    ..The J-binding proteins show 43-63% identity and are unlike any known protein. The discovery of a J-binding protein suggests that J, like methylated cytosine in higher eukaryotes, functions via a protein intermediate...
  34. ncbi request reprint A family of drug transporters: the multidrug resistance-associated proteins
    P Borst
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam
    J Natl Cancer Inst 92:1295-302. 2000
    ..The physiologic functions of the other MRPs are not known. Whether long-term inhibition of MRPs in humans can be tolerated (assuming that suitable inhibitors will be found) remains to be determined...
  35. ncbi request reprint Characterization of drug transport by the human multidrug resistance protein 3 (ABCC3)
    N Zelcer
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 276:46400-7. 2001
    ..Even though etoposide glucuronide is an excellent substrate for MRP3, this compound is not involved in the etoposide resistance of our MRP3 cells, as these cells extrude unmodified etoposide rather than etoposide glucuronide...
  36. ncbi request reprint Selection for activation of a new variant surface glycoprotein gene expression site in Trypanosoma brucei can result in deletion of the old one
    G Rudenko
    Department of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    Mol Biochem Parasitol 95:97-109. 1998
    ..The fact that we pick up these normally infrequent deletions, indicates that inactivation of the old VSG expression site could be rate limiting during switching in our strain of T. brucei...
  37. pmc Multidrug resistance protein 1 protects the oropharyngeal mucosal layer and the testicular tubules against drug-induced damage
    J Wijnholds
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    J Exp Med 188:797-808. 1998
    ..Our results indicate that specific inhibitors of MRP1 used to reverse MDR, in combination with carcinostatic drugs transported by MRP1, might lead to drug-induced mucositis, (temporary) infertility, and diabetes insipidus...
  38. pmc Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces
    A van Helvoort
    Division of Molecular Biology, Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 96:11501-6. 1999
    ..We discuss how PC might reach the canalicular membrane of the hepatocyte for secretion into the bile, if not by Pc-tp...
  39. ncbi request reprint Genetic dissection of the function of mammalian P-glycoproteins
    P Borst
    The Netherlands Cancer Institute, Department of Molecular Biology, Amsterdam, The Netherlands
    Trends Genet 13:217-22. 1997
    ..Recent experiments with polarized cells support the idea that drug-transporting P-glycoproteins act by flipping drugs from the inner to the outer leaflet of the plasma membrane...
  40. ncbi request reprint Transport of glutathione prostaglandin A conjugates by the multidrug resistance protein 1
    R Evers
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    FEBS Lett 419:112-6. 1997
    ..We conclude that mouse erythrocytes contain at least two transport systems for PGA2-GS. One of these is mrp1; the other one has not been identified yet, but can be inhibited by methotrexate and cAMP...
  41. pmc Vinblastine and sulfinpyrazone export by the multidrug resistance protein MRP2 is associated with glutathione export
    R Evers
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam
    Br J Cancer 83:375-83. 2000
    ..However, at high sulfinpyrazone concentrations this compound is transported without GSH. Models of MRP action are discussed that could explain these results...
  42. ncbi request reprint Expression of human MRP6, a homologue of the multidrug resistance protein gene MRP1, in tissues and cancer cells
    M Kool
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    Cancer Res 59:175-82. 1999
    ..Our results suggest that MRP6 does not play a role in the resistance of the resistant cells analyzed, and that MRP6/ARA is only coamplified with MRP1 because of its location immediately next to it on the same chromosome...
  43. pmc Biosynthesis and function of the modified DNA base beta-D-glucosyl-hydroxymethyluracil in Trypanosoma brucei
    F van Leeuwen
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Mol Cell Biol 18:5643-51. 1998
    ..We speculate that these effects are mediated by the packaging of J-containing DNA into a condensed chromatin structure...
  44. ncbi request reprint Hepatocyte-specific expression of the human MDR3 P-glycoprotein gene restores the biliary phosphatidylcholine excretion absent in Mdr2 (-/-) mice
    A J Smith
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    Hepatology 28:530-6. 1998
    ..The excretion of cholesterol is not tightly coupled to the excretion of phospholipids in these mice, because a very low phospholipid excretion level is sufficient to give almost wild-type cholesterol excretion into the bile...
  45. ncbi request reprint Tissue distribution of the human MDR3 P-glycoprotein
    J J Smit
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam
    Lab Invest 71:638-49. 1994
    ..The MDR1 Pgp can transport drugs; the murine homologue of MDR3, mdr2, was recently shown by us to be involved in transport of the phospholipid phosphatidylcholine (lecithin) into bile...
  46. ncbi request reprint Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies
    G L Scheffer
    Department of Pathology, Free University Hospital, Amsterdam, The Netherlands
    Cancer Res 60:5269-77. 2000
    ....
  47. ncbi request reprint Mice lacking the multidrug resistance protein 1 are resistant to Streptococcus pneumoniae-induced pneumonia
    M J Schultz
    Laboratory of Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Immunol 166:4059-64. 2001
    ..We conclude that mrp1(-/-) mice are resistant against pneumococcal pneumonia by a mechanism that involves increased release of LTB(4). These results identify mrp1 as a novel target for adjunctive therapy in pneumonia...
  48. ncbi request reprint Multidrug resistance and the role of P-glycoprotein knockout mice
    A H Schinkel
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam, The Netherlands
    Eur J Cancer 31:1295-8. 1995
    ..For some drugs, this leads to dramatically increased toxicity, indicating that P-glycoprotein inhibitors should be used with caution in patients...
  49. pmc Enhanced oral bioavailability of paclitaxel in mice treated with the P-glycoprotein blocker SDZ PSC 833
    J van Asperen
    Department of Clinical Chemistry, The Netherlands Cancer Institute, Amsterdam
    Br J Cancer 76:1181-3. 1997
    ..These results encourage further research on the development of a clinically useful oral formulation of paclitaxel...
  50. ncbi request reprint Thiopurine metabolism and identification of the thiopurine metabolites transported by MRP4 and MRP5 overexpressed in human embryonic kidney cells
    P R Wielinga
    Division of Molecular Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Mol Pharmacol 62:1321-31. 2002
    ..Our results show that all major thiopurine monophosphates important in the efficacy of mercaptopurine treatment are transported by MRP4 and MRP5, although the substrate specificity of the two transporters differs in detail...
  51. ncbi request reprint Sequence of mdr3 cDNA encoding a human P-glycoprotein
    A M van der Bliek
    The Netherlands Cancer Institute, Department of Molecular Biology, Amsterdam
    Gene 71:401-11. 1988
    ..80% of the amino acids are conserved between the products of mdr1 and mdr3. Although the function of mdr3 is not yet known, its high homology with mdr1 suggests that it also encodes an efflux pump with broad specificity...
  52. ncbi request reprint Genes amplified and overexpressed in human multidrug-resistant cell lines
    A M van der Bliek
    Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam
    Cancer Res 48:5927-32. 1988
    ..Our results confirm the central role of the mdr1 (pgp1) gene in MDR and suggest that different cross-resistance patterns are not due to differential expression of different P-glycoprotein genes...
  53. pmc Substantial excretion of digoxin via the intestinal mucosa and prevention of long-term digoxin accumulation in the brain by the mdr 1a P-glycoprotein
    U Mayer
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Br J Pharmacol 119:1038-44. 1996
    ....
  54. pmc Reduced topoisomerase II activity in multidrug-resistant human non-small cell lung cancer cell lines
    E W Eijdems
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    Br J Cancer 71:40-7. 1995
    ..The assay we used, which measures DNA breaks as an end point of topo II activity, could be a good candidate...
  55. pmc The human mdr3 gene encodes a novel P-glycoprotein homologue and gives rise to alternatively spliced mRNAs in liver
    A M van der Bliek
    Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam
    EMBO J 6:3325-31. 1987
    ....
  56. ncbi request reprint Changing the end: antigenic variation orchestrated at the telomeres of African trypanosomes
    G Rudenko
    Dept of Molecular Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Trends Microbiol 6:113-6. 1998
    ..This genomic location at chromosome ends not only allows easy exchange of VSG gene cassettes using various mechanisms of DNA recombination but also appears to play a role in VSG gene expression site control...
  57. ncbi request reprint A mutation in the human canalicular multispecific organic anion transporter gene causes the Dubin-Johnson syndrome
    C C Paulusma
    Department of Gastrointestinal and Liver Diseases, Center for Liver and Intestinal Research, Academic Medical Center, Amsterdam, The Netherlands
    Hepatology 25:1539-42. 1997
    ..In the present study, we have isolated the human homologue of rat cmoat, human cMOAT, and analyzed the corresponding cDNA from fibroblasts of a DJS patient for mutations. Our results show that a mutation in this gene is the cause of DJS...
  58. ncbi request reprint What have we learnt thus far from mice with disrupted P-glycoprotein genes?
    P Borst
    Division of Molecular Biology, Netherlands Cancer Institute
    Eur J Cancer 32:985-90. 1996
  59. ncbi request reprint Control of VSG gene expression sites
    P Borst
    The Netherlands Cancer Institute, Division of Molecular Biology and Centre of Biomedical Genetics, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Mol Biochem Parasitol 114:17-27. 2001
    ..This review summarizes recent work on the mechanism of site switching and on the way inactive expression sites are kept silent...
  60. ncbi request reprint Altered pharmacokinetics of vinblastine in Mdr1a P-glycoprotein-deficient Mice
    J van Asperen
    Department of Clinical Chemistry, The Netherlands Cancer Institute, Amsterdan, The Netherlands
    J Natl Cancer Inst 88:994-9. 1996
    ..Analysis of the consequences of Pgp blockade has been facilitated by the generation of mice with disrupted mdr1a genes [mdr1a(-/-)]...
  61. ncbi request reprint J-binding protein increases the level and retention of the unusual base J in trypanosome DNA
    Mike Cross
    The Netherlands Cancer Institute, Division of Molecular Biology and Center for Biomedical Genetics, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Mol Microbiol 46:37-47. 2002
    ..We conclude that JBP is able to activate the thymine modification enzymes to introduce additional J in regions of DNA already containing a basal level of J. We propose that JBP is a novel DNA modification maintenance protein...
  62. pmc Expression of the human DNA glycosylase hSMUG1 in Trypanosoma brucei causes DNA damage and interferes with J biosynthesis
    Sebastian Ulbert
    Department of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nucleic Acids Res 30:3919-26. 2002
    ..hSMUG1 also reduces the J content of the trypanosome DNA. This work supports the idea that HOMeUra is a precursor of J, freely accessible to a DNA glycosylase...
  63. ncbi request reprint Recognition of base J in duplex DNA by J-binding protein
    Robert Sabatini
    Division of Molecular Biology and Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 277:958-66. 2002
    ..We conclude that JBP is a structure-specific DNA-binding protein. The significance of these results in relation to the biological role and mechanism of action of J modification in kinetoplastids is discussed...
  64. doi request reprint Evidence that J-binding protein 2 is a thymidine hydroxylase catalyzing the first step in the biosynthesis of DNA base J
    Saara Vainio
    The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, The Netherlands
    Mol Biochem Parasitol 164:157-61. 2009
    ..We conclude that JBP2, like JBP1, is in all probability a thymidine hydroxylase involved in the biosynthesis of base J...
  65. pmc ABCC6 prevents ectopic mineralization seen in pseudoxanthoma elasticum by inducing cellular nucleotide release
    Robert S Jansen
    Divisions of Molecular Oncology and Gene Regulation, Netherlands Cancer Institute, 1066 CX, Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 110:20206-11. 2013
    ..Our data indicate that the factor that normally prevents PXE is PPi, which is provided to the circulation in the form of nucleoside triphosphates via an as-yet unidentified but ABCC6-dependent mechanism. ..
  66. pmc Controlled turnover and 3' trimming of the trans splicing precursor of Trypanosoma brucei
    P W Laird
    Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam
    Nucleic Acids Res 15:10087-103. 1987
    ....
  67. ncbi request reprint Genetic mechanisms of drug resistance. A review
    P Borst
    The Netherlands Cancer Institute, Amsterdam
    Acta Oncol 30:87-105. 1991
    ....
  68. pmc cDNA cloning and tissue-specific expression of the phosphatidylcholine transfer protein gene
    T B Geijtenbeek
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Biochem J 316:49-55. 1996
    ..In addition to adult liver, high levels of PC-TP RNA were also found in kidney and testis. The prominent presence of PC-TP in developing and adult liver is compatible with its proposed role in bile formation...
  69. ncbi request reprint A minor fraction of base J in kinetoplastid nuclear DNA is bound by the J-binding protein 1
    Cristiane Bentin Toaldo
    The Netherlands Cancer Institute, Division of Molecular Biology and Centre of Biomedical Genetics, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Mol Biochem Parasitol 143:111-5. 2005
  70. pmc Absence of the mdr1a P-Glycoprotein in mice affects tissue distribution and pharmacokinetics of dexamethasone, digoxin, and cyclosporin A
    A H Schinkel
    The Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam, The Netherlands
    J Clin Invest 96:1698-705. 1995
    ..These results may explain a range of pharmacological interactions observed between various drugs in patients...
  71. pmc The identification of hydroxymethyluracil in DNA of Trypanosoma brucei
    J H Gommers-Ampt
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    Nucleic Acids Res 21:2039-43. 1993
    ..Because of its high sensitivity, it may also be useful for the detection of the low amounts of HOMedU resulting from oxidative damage of DNA...
  72. doi request reprint Sensitivity and acquired resistance of BRCA1;p53-deficient mouse mammary tumors to the topoisomerase I inhibitor topotecan
    Serge A L Zander
    Division of Molecular Biology, The Netherlands Cancer Institute, 1066CX Amsterdam, The Netherlands
    Cancer Res 70:1700-10. 2010
    ..We find that olaparib substantially increases topotecan toxicity in this model, and we suggest that this might also happen in humans...
  73. pmc High sensitivity of BRCA1-deficient mammary tumors to the PARP inhibitor AZD2281 alone and in combination with platinum drugs
    Sven Rottenberg
    Division of Molecular Biology and Centre for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 105:17079-84. 2008
    ....
  74. doi request reprint Contribution of the drug transporter ABCG2 (breast cancer resistance protein) to resistance against anticancer nucleosides
    Cornelia de Wolf
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, 1066 CX The Netherlands
    Mol Cancer Ther 7:3092-102. 2008
    ....
  75. ncbi request reprint Base J, found in nuclear DNA of Trypanosoma brucei, is not a target for DNA glycosylases
    Sebastian Ulbert
    Division of Molecular Biology, Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, CX Amsterdam 1066, The Netherlands
    DNA Repair (Amst) 3:145-54. 2004
    ..The presence of J in DNA does not require a specific modification of the BER system, as this base is not recognized by any known DNA glycosylase...
  76. ncbi request reprint Expression site activation in Trypanosoma brucei with three marked variant surface glycoprotein gene expression sites
    Sebastian Ulbert
    Department of Molecular Biology and Centre of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Mol Biochem Parasitol 120:225-35. 2002
    ..Whereas, a "silent" ES shows a low level of expression of promoter proximal sequences, the level of activation can be reversibly increased, leading to partially activated ESs...
  77. pmc Steroid and bile acid conjugates are substrates of human multidrug-resistance protein (MRP) 4 (ATP-binding cassette C4)
    Noam Zelcer
    Division of Molecular Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Biochem J 371:361-7. 2003
    ..Our findings suggest a physiological role for MRP1 and MRP4 in DHEAS transport and an involvement of MRP4 in transport of conjugated steroids and bile acids...
  78. doi request reprint Moderate increase in Mdr1a/1b expression causes in vivo resistance to doxorubicin in a mouse model for hereditary breast cancer
    Marina Pajic
    Division of Molecular Biology and Centre for Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Cancer Res 69:6396-404. 2009
    ..Our data show the usefulness of realistic preclinical models to characterize levels of Mdr1 gene expression that are sufficient to cause resistance...
  79. ncbi request reprint The expression level determines the surface distribution of the transferrin receptor in Trypanosoma brucei
    Rainer Mussmann
    Division of Molecular Biology, and Center for Biomedical Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Mol Microbiol 47:23-35. 2003
    ..These results suggest that the TfR flagellar pocket retention mechanism is easily saturated and that control of the expression level is critical to maintain the restricted surface distribution of the receptor...
  80. ncbi request reprint The effect of low pH on breast cancer resistance protein (ABCG2)-mediated transport of methotrexate, 7-hydroxymethotrexate, methotrexate diglutamate, folic acid, mitoxantrone, topotecan, and resveratrol in in vitro drug transport models
    Pauline Breedveld
    Department of Experimental Therapy, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Mol Pharmacol 71:240-9. 2007
    ..4, is efficiently transported by BCRP at pH 6.0, whereas we did not detect active transport at pH 7.4. We conclude that BCRP transports substrate drugs more efficiently at low pH, independent of the dissociation status of the substrate...
  81. pmc Selective induction of chemotherapy resistance of mammary tumors in a conditional mouse model for hereditary breast cancer
    Sven Rottenberg
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 104:12117-22. 2007
    ..Our results underline the promise of these mouse tumors for the study of tumor-initiating cells and of drug therapy of human cancer...
  82. ncbi request reprint Trypanosomes change their transferrin receptor expression to allow effective uptake of host transferrin
    Henri G A M van Luenen
    The Netherlands Cancer Institute, Division of Molecular Biology and Centre for Biomedical Genetics, Plesmanlaan 121, 1060 CX Amsterdam, The Netherlands
    Mol Microbiol 58:151-65. 2005
    ..Our results illustrate the striking genetic flexibility of trypanosomes...
  83. ncbi request reprint Structure of the human MDR3 gene and physical mapping of the human MDR locus
    C R Lincke
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam
    J Biol Chem 266:5303-10. 1991
    ..The CpG-rich sequences marking the 5' ends of both genes are hypomethylated to different extents in different cell lines. Hypomethylation roughly correlates with transcriptional activity...
  84. pmc Multidrug resistance-associated protein 9 (ABCC12) is present in mouse and boar sperm
    Nobuhito Ono
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Biochem J 406:31-40. 2007
    ..In HEK-293 cells, it is predominantly localized in the endoplasmic reticulum. We have been unable to demonstrate transport by MRP9 of substrates transported by other MRPs, such as drug conjugates and other organic anions...
  85. ncbi request reprint Multidrug resistance proteins 2 and 3 provide alternative routes for hepatic excretion of morphine-glucuronides
    Koen van de Wetering
    Division of Molecular Biology, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Mol Pharmacol 72:387-94. 2007
    ..Our results show that murine Mrp2 and Mrp3 provide alternative routes for the excretion of a glucuronidated substrate from the liver in vivo...
  86. ncbi request reprint cGMP transport by vesicles from human and mouse erythrocytes
    Cornelia J F De Wolf
    Department of Molecular Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    FEBS J 274:439-50. 2007
    ..The relative contribution of ABCC4/Abcc4 and ABCG2/Abcg2 in cGMP transport was confirmed with a new inhibitor of ABCC4 transport, the protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride...
  87. ncbi request reprint Characterization of the MRP4- and MRP5-mediated transport of cyclic nucleotides from intact cells
    Peter R Wielinga
    Division of Molecular Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands
    J Biol Chem 278:17664-71. 2003
    ....
  88. ncbi request reprint Tissue distribution and induction of human multidrug resistant protein 3
    George L Scheffer
    Department of Pathology, Free University Medical Center, Amsterdam, The Netherlands
    Lab Invest 82:193-201. 2002
    ..These findings may be of potential clinical relevance when considering the drug treatment regimens for these tumor types...
  89. ncbi request reprint The physiological significance of transferrin receptor variations in Trypanosoma brucei
    Herlinde Gerrits
    The Netherlands Cancer Institute, Division of Molecular Biology and Center for Biomedical Genetics, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Mol Biochem Parasitol 119:237-47. 2002
    ..Our results suggest that a high-affinity Tf-R not only ensures efficient Tf uptake, but is also required to allow sufficient iron uptake by the trypanosome in the presence of anti-Tf-R antibodies...
  90. ncbi request reprint Mice lacking Mrp3 (Abcc3) have normal bile salt transport, but altered hepatic transport of endogenous glucuronides
    Noam Zelcer
    Division of Molecular Biology, H8, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Hepatol 44:768-75. 2006
    ..Multidrug Resistance Protein 3 (MRP3) transports bile salts and glucuronide conjugates in vitro and is postulated to protect the liver in cholestasis. Whether the absence of Mrp3 affects these processes in vivo is tested...
  91. ncbi request reprint The human multidrug resistance protein MRP5 transports folates and can mediate cellular resistance against antifolates
    Peter Wielinga
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam
    Cancer Res 65:4425-30. 2005
    ..These results show the potential of MRP5 to mediate transport of (anti)folates and contribute to resistance against antifolate drugs...
  92. pmc Mice lacking multidrug resistance protein 3 show altered morphine pharmacokinetics and morphine-6-glucuronide antinociception
    Noam Zelcer
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 102:7274-9. 2005
    ..The pharamacokinetics of injected morphine-glucuronides are altered as well in the absence of Mrp3, and this results in a decreased antinociceptive potency of injected morphine-6-glucuronide...
  93. ncbi request reprint Cancer cell death by programmed necrosis?
    Piet Borst
    Division of Molecular Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, Netherlands
    Drug Resist Updat 7:321-4. 2004
    ....
  94. doi request reprint Intestinal breast cancer resistance protein (BCRP)/Bcrp1 and multidrug resistance protein 3 (MRP3)/Mrp3 are involved in the pharmacokinetics of resveratrol
    Koen van de Wetering
    Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Mol Pharmacol 75:876-85. 2009
    ..The profound effects of ATP-binding cassette transporters on the disposal of resveratrol may be representative for the handling of several other polyphenols of dietary origin...
  95. ncbi request reprint Analysis of telomere length variation in Leishmania over time
    Paul André Genest
    The Netherlands Cancer Institute, Divison of Molecular Biology and Centre of Biomedical Genetics, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Mol Biochem Parasitol 151:213-5. 2007
  96. ncbi request reprint Characterization of the transport of nucleoside analog drugs by the human multidrug resistance proteins MRP4 and MRP5
    Glen Reid
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Mol Pharmacol 63:1094-103. 2003
    ..These results strongly suggest that the affinity of MRP4 and MRP5 for nucleotide-based substrates is low...
  97. ncbi request reprint Evidence for two interacting ligand binding sites in human multidrug resistance protein 2 (ATP binding cassette C2)
    Noam Zelcer
    Division of Molecular Biology and Center of Biomedical Genetics, Netherlands Cancer Institute, Amsterdam, The Netherlands
    J Biol Chem 278:23538-44. 2003
    ..We propose that MRP2 contains two similar but nonidentical ligand binding sites: one site from which substrate is transported and a second site that regulates the affinity of the transport site for the substrate...
  98. pmc The human multidrug resistance protein MRP4 functions as a prostaglandin efflux transporter and is inhibited by nonsteroidal antiinflammatory drugs
    Glen Reid
    Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 100:9244-9. 2003
    ..Together, these data suggest that MRP4 can release prostaglandins from cells, and that, in addition to inhibiting prostaglandin synthesis, some nonsteroidal antiinflammatory drugs might also act by inhibiting this release...
  99. ncbi request reprint Factors affecting the level and localization of the transferrin receptor in Trypanosoma brucei
    Rainer Mussmann
    The Netherlands Cancer Institute, Division of Molecular Biology, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 279:40690-8. 2004
    ..This flexibility is made possible by the promoter-proximal position of the two genes in the variant surface glycoprotein expression site...
  100. ncbi request reprint Mechanism of the pharmacokinetic interaction between methotrexate and benzimidazoles: potential role for breast cancer resistance protein in clinical drug-drug interactions
    Pauline Breedveld
    Divisions of Experimental Therapy, Molecular Biology, Clinical Chemistry, and Medical Oncology, The Netherlands Cancer Institute, Amsterdam
    Cancer Res 64:5804-11. 2004
    ..v. MTX in wild-type mice. The conclusion is as follows: benzimidazoles differentially affect transport of MTX mediated by BCRP and MRP2. Competition for BCRP may explain the clinical interaction between MTX and benzimidazoles...
  101. ncbi request reprint Role of the N-terminal transmembrane region of the multidrug resistance protein MRP2 in routing to the apical membrane in MDCKII cells
    Sara B Mateus Fernández
    Georg Speyer Haus, Paul Ehrlich Strasse 42 44, 60596 Frankfurt am Main, Germany
    J Biol Chem 277:31048-55. 2002
    ..Our results suggest that the TMD(0) region is required for routing to or stable association with the apical membrane...