Research Topics
| Harold TjalsmaSummaryAffiliation: Radboud University Nijmegen Medical Centre Country: The Netherlands Publications
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Detail Information
Publications
A bacterial driver-passenger model for colorectal cancer: beyond the usual suspectsHarold Tjalsma
Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i and Radboud University Centre for Oncology RUCO, Geert Grooteplein 10 830, 6525 GA Nijmegen, The Netherlands
Nat Rev Microbiol 10:575-82. 2012..Here, we propose a bacterial driver-passenger model for microbial involvement in the development of colorectal cancer and suggest that this model be incorporated into the genetic paradigm of cancer progression...- Identification of biomarkers for colorectal cancer through proteomics-based approachesHarold Tjalsma
Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Expert Rev Proteomics 7:879-95. 2010..Eventually, multimarker assays should be developed to reach a diagnostic accuracy that meets the stringent criteria for colorectal cancer screening at the population level... - Clinical Importance of Streptococcus gallolyticus infection among colorectal cancer patients: systematic review and meta-analysisAnnemarie Boleij
Department of Laboratory Medicine 830, Nijmegen Institute for Infection, Inflammation and Immunity N4i, The Netherlands
Clin Infect Dis 53:870-8. 2011..With this systematic review, we aim to increase the awareness of the association between S. bovis biotype I (Streptococcus gallolyticus) and CRC and urge for uniform molecular microbiological classification... - Secretion of bioactive hepcidin-25 by liver cells correlates with its gene transcription and points towards synergism between iron and inflammation signaling pathwaysApriliana E R Kartikasari
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Biochim Biophys Acta 1784:2029-37. 2008..The study underscores hepcidin-25 quantification as a valuable tool to unravel regulatory pathways in iron metabolism... 
Bacterial responses to a simulated colon tumor microenvironmentAnnemarie Boleij
Department of Laboratory Medicine 830, Radboud University Medical Centre, 6500 HB Nijmegen, The Netherlands
Mol Cell Proteomics 11:851-62. 2012..gallolyticus, favoring its local outgrowth and providing a possible explanation for the specific association of S. gallolyticus with colonic malignancy...- Hepcidin in human iron disorders: diagnostic implicationsJoyce J C Kroot
Department of Laboratory Medicine, Laboratory of Genetic, Endocrine and Metabolic Disorders, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Clin Chem 57:1650-69. 2011..The measurement of hepcidin in biological fluids is therefore a promising tool in the diagnosis and management of medical conditions in which iron metabolism is affected... - Increased exposure to bacterial antigen RpL7/L12 in early stage colorectal cancer patientsAnnemarie Boleij
Department of Laboratory Medicine Clinical Chemistry, Nijmegen Institute for Infection, Inflammation, and Immunity, Radboud University Center for Oncology of the Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
Cancer 116:4014-22. 2010..This work aims to investigate the humoral immune response to this bacterium during different stages of colorectal cancer... - Immunochemical and mass-spectrometry-based serum hepcidin assays for iron metabolism disordersJoyce J C Kroot
Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Clin Chem 56:1570-9. 2010..Hepcidin is instrumental in the diagnosis and monitoring of iron metabolism disorders, but reliable methods for its quantification in serum are sparse, as is knowledge of their relative analytical strengths and clinical utility... 
Regulation of hepcidin: insights from biochemical analyses on human serum samplesErwin H J M Kemna
Department of Clinical Chemistry 441, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
Blood Cells Mol Dis 40:339-46. 2008..Future studies are needed to validate the combined utility of this predictive algorithm together with actual measured hepcidin levels in clinical diagnosis...- Second round robin for plasma hepcidin methods: first steps toward harmonizationJoyce J C Kroot
Department of Laboratory Medicine, Laboratory of Genetic Endocrine and Metabolic Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Am J Hematol 87:977-83. 2012..This study represents the first step toward harmonization of plasma hepcidin methods and facilitates aggregation of hepcidin data from different research investigations... - Mass spectrometry analysis of hepcidin peptides in experimental mouse modelsHarold Tjalsma
Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
PLoS ONE 6:e16762. 2011.... - Novel clues on the specific association of Streptococcus gallolyticus subsp gallolyticus with colorectal cancerAnnemarie Boleij
Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
J Infect Dis 203:1101-9. 2011..Therefore, this study aimed to reveal the virulence characteristics that distinguish this opportunistic pathogen from a panel of (closely related) intestinal bacteria... - Surface-affinity profiling to identify host-pathogen interactionsAnnemarie Boleij
Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity and Radboud University Centrefor Oncology of the Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
Infect Immun 79:4777-83. 2011..In conclusion, our study shows that surface-affinity profiling is a valuable tool to identify novel adhesin-receptor pairs, which advocates its application in other hybrid biological systems... - Surface-exposed histone-like protein a modulates adherence of Streptococcus gallolyticus to colon adenocarcinoma cellsAnnemarie Boleij
Department of Laboratory Medicine, Nijmegen Institutes for Infection, Inflammation and Immunity and Oncology of the Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands
Infect Immun 77:5519-27. 2009..This may be explained by the fact that heparan sulfate and LTA compete for the same binding site in HlpA. Altogether, this study implies that HlpA serves as a fine-tuning factor for bacterial adherence... - (Pre)analytical imprecision, between-subject variability, and daily variations in serum and urine hepcidin: implications for clinical studiesJoyce J C Kroot
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Anal Biochem 389:124-9. 2009..Transferrin saturation did not correlate with hepcidin levels. To minimize variability, we recommend (i) standardizing for sampling time and (ii) measuring serum hepcidin levels... - Advances in quantitative hepcidin measurements by time-of-flight mass spectrometryDorine W Swinkels
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
PLoS ONE 3:e2706. 2008.... - Selective antibody response to Streptococcus gallolyticus pilus proteins in colorectal cancer patientsAnnemarie Boleij
Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i, Nijmegen, The Netherlands
Cancer Prev Res (Phila) 5:260-5. 2012..Together these findings underscore the potential of a multi-antigen approach to complement diagnosis of S. gallolyticus subsp. gallolyticus-associated CRC... - High-sensitive radioimmunoassay for human serum hepcidinNicolai Grebenchtchikov
Department of Chemical Endocrinology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Br J Haematol 146:317-25. 2009.... - Hepcidin: from discovery to differential diagnosisErwin H J M Kemna
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, P O Box 9101 6500 HB Nijmegen, The Netherlands
Haematologica 93:90-7. 2008..Ongoing studies in humans should provide us with more information on the etiology of iron metabolism disorders in order to create new therapeutic strategies and improve differential diagnosis protocols for these diseases... - Shedding & shaving: disclosure of proteomic expressions on a bacterial faceHarold Tjalsma
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Proteomics 8:1415-28. 2008..Together this shows that these combined gel-free approaches can reveal novel proteomic decorations on a bacterial face and may provide leads for future studies on protein sorting in B. subtilis and other Gram-positive bacteria... - Proteomic inventory of "anchorless" proteins on the colon adenocarcinoma cell surfaceHarold Tjalsma
Department of Clinical Chemistry, 441, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
Biochim Biophys Acta 1764:1607-17. 2006.... - Iron availability increases the pathogenic potential of Salmonella typhimurium and other enteric pathogens at the intestinal epithelial interfaceGuus A M Kortman
Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
PLoS ONE 7:e29968. 2012..In conclusion, our data fit with the consensus that oral iron supplementation is not without risk as iron could, in addition to inducing pathogenic overgrowth, also increase the virulence of prevalent enteric pathogens... - Nitric oxide does not contribute to inflammation-induced hypoferremia in humansErwin H J M Kemna
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Acta Haematol 118:149-52. 2007 - Novel urine hepcidin assay by mass spectrometryErwin Kemna
Department of Clinical Chemistry 564, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands
Blood 106:3268-70. 2005..In conclusion, this novel MS urine hepcidin assay is easy to perform and available to a wide audience. This enables the implementation of hepcidin measurements in large clinical studies... - Feature-based reappraisal of the Bacillus subtilis exoproteomeHarold Tjalsma
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Proteomics 7:73-81. 2007..Finally, the present study suggests that feature-based outlier analysis may provide leads towards the discovery of novel special-purpose pathways for bacterial protein export... - Simultaneous proteomic and genomic analysis of primary Ta urothelial cell carcinomas for the prediction of tumor recurrenceIman J Schultz
Departments of Clinical Chemistry, Radboud University Nijmegen Medical Center, PO Box 9101, 6500 HB Nijmegen, The Netherlands
Anticancer Res 27:1051-8. 2007..The prediction of tumor recurrence in patients with Ta urothelial cell carcinoma is inaccurate and new prognostic markers are desirable... - Gut bacteria in health and disease: a survey on the interface between intestinal microbiology and colorectal cancerAnnemarie Boleij
Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i and Radboud University Centre for Oncology RUCO of the Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
Biol Rev Camb Philos Soc 87:701-30. 2012..A thorough understanding of these processes will provide leads for future research and may ultimately aid in development of new strategies for CRC diagnosis and prevention... - Immunoproteomics: From biomarker discovery to diagnostic applicationsHarold Tjalsma
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Proteomics Clin Appl 2:167-80. 2008.... - Profiling the humoral immune response in colon cancer patients: diagnostic antigens from Streptococcus bovisHarold Tjalsma
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
Int J Cancer 119:2127-35. 2006..This makes profiling of the humoral immune response against "silent" S. bovis infections a promising diagnostic tool for the early detection of human colon cancer, which is crucial for the effective treatment of this disease... - Proteomics-based consensus prediction of protein retention in a bacterial membraneHarold Tjalsma
Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, The Netherlands
Proteomics 5:4472-82. 2005..Our findings provide important leads for future identification of surface proteins from pathogenic bacteria, which are attractive candidate infection markers and potential targets for drugs or vaccines... - Mass spectrometry-based hepcidin measurements in serum and urine: analytical aspects and clinical implicationsErwin H J M Kemna
Department of Clinical Chemistry, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
Clin Chem 53:620-8. 2007..Recent reports on the measurement of urine and serum hepcidin by surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) necessitate analytical and clinical evaluation of MS-based methodologies...