Harold Tjalsma

Summary

Affiliation: Radboud University Nijmegen Medical Centre
Country: The Netherlands

Publications

  1. pmc Iron availability increases the pathogenic potential of Salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface
    Guus A M Kortman
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    PLoS ONE 7:e29968. 2012
  2. doi request reprint A bacterial driver-passenger model for colorectal cancer: beyond the usual suspects
    Harold Tjalsma
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i and Radboud University Centre for Oncology RUCO, Geert Grooteplein 10 830, 6525 GA Nijmegen, The Netherlands
    Nat Rev Microbiol 10:575-82. 2012
  3. doi request reprint Clinical Importance of Streptococcus gallolyticus infection among colorectal cancer patients: systematic review and meta-analysis
    Annemarie Boleij
    Department of Laboratory Medicine 830, Nijmegen Institute for Infection, Inflammation and Immunity N4i, The Netherlands
    Clin Infect Dis 53:870-8. 2011
  4. doi request reprint Identification of biomarkers for colorectal cancer through proteomics-based approaches
    Harold Tjalsma
    Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Expert Rev Proteomics 7:879-95. 2010
  5. doi request reprint Secretion of bioactive hepcidin-25 by liver cells correlates with its gene transcription and points towards synergism between iron and inflammation signaling pathways
    Apriliana E R Kartikasari
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Biochim Biophys Acta 1784:2029-37. 2008
  6. doi request reprint Diurnal rhythm rather than dietary iron mediates daily hepcidin variations
    Charlotte C M Schaap
    Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine, Radboud University Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Clin Chem 59:527-35. 2013
  7. pmc Bacterial responses to a simulated colon tumor microenvironment
    Annemarie Boleij
    Department of Laboratory Medicine 830, Radboud University Medical Centre, 6500 HB Nijmegen, The Netherlands
    Mol Cell Proteomics 11:851-62. 2012
  8. doi request reprint Hepcidin in human iron disorders: diagnostic implications
    Joyce J C Kroot
    Department of Laboratory Medicine, Laboratory of Genetic, Endocrine and Metabolic Disorders, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Chem 57:1650-69. 2011
  9. pmc Surface-exposed histone-like protein a modulates adherence of Streptococcus gallolyticus to colon adenocarcinoma cells
    Annemarie Boleij
    Department of Laboratory Medicine, Nijmegen Institutes for Infection, Inflammation and Immunity and Oncology of the Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands
    Infect Immun 77:5519-27. 2009
  10. pmc Increased exposure to bacterial antigen RpL7/L12 in early stage colorectal cancer patients
    Annemarie Boleij
    Department of Laboratory Medicine Clinical Chemistry, Nijmegen Institute for Infection, Inflammation, and Immunity, Radboud University Center for Oncology of the Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Cancer 116:4014-22. 2010

Collaborators

Detail Information

Publications36

  1. pmc Iron availability increases the pathogenic potential of Salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface
    Guus A M Kortman
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    PLoS ONE 7:e29968. 2012
    ..In conclusion, our data fit with the consensus that oral iron supplementation is not without risk as iron could, in addition to inducing pathogenic overgrowth, also increase the virulence of prevalent enteric pathogens...
  2. doi request reprint A bacterial driver-passenger model for colorectal cancer: beyond the usual suspects
    Harold Tjalsma
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i and Radboud University Centre for Oncology RUCO, Geert Grooteplein 10 830, 6525 GA Nijmegen, The Netherlands
    Nat Rev Microbiol 10:575-82. 2012
    ..Here, we propose a bacterial driver-passenger model for microbial involvement in the development of colorectal cancer and suggest that this model be incorporated into the genetic paradigm of cancer progression...
  3. doi request reprint Clinical Importance of Streptococcus gallolyticus infection among colorectal cancer patients: systematic review and meta-analysis
    Annemarie Boleij
    Department of Laboratory Medicine 830, Nijmegen Institute for Infection, Inflammation and Immunity N4i, The Netherlands
    Clin Infect Dis 53:870-8. 2011
    ..With this systematic review, we aim to increase the awareness of the association between S. bovis biotype I (Streptococcus gallolyticus) and CRC and urge for uniform molecular microbiological classification...
  4. doi request reprint Identification of biomarkers for colorectal cancer through proteomics-based approaches
    Harold Tjalsma
    Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Expert Rev Proteomics 7:879-95. 2010
    ..Eventually, multimarker assays should be developed to reach a diagnostic accuracy that meets the stringent criteria for colorectal cancer screening at the population level...
  5. doi request reprint Secretion of bioactive hepcidin-25 by liver cells correlates with its gene transcription and points towards synergism between iron and inflammation signaling pathways
    Apriliana E R Kartikasari
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Biochim Biophys Acta 1784:2029-37. 2008
    ..The study underscores hepcidin-25 quantification as a valuable tool to unravel regulatory pathways in iron metabolism...
  6. doi request reprint Diurnal rhythm rather than dietary iron mediates daily hepcidin variations
    Charlotte C M Schaap
    Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine, Radboud University Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Clin Chem 59:527-35. 2013
    ..It is currently unknown whether these increases are determined by an innate rhythm or by other factors. We aimed to assess the effect of dietary iron on hepcidin concentrations during the day...
  7. pmc Bacterial responses to a simulated colon tumor microenvironment
    Annemarie Boleij
    Department of Laboratory Medicine 830, Radboud University Medical Centre, 6500 HB Nijmegen, The Netherlands
    Mol Cell Proteomics 11:851-62. 2012
    ..gallolyticus, favoring its local outgrowth and providing a possible explanation for the specific association of S. gallolyticus with colonic malignancy...
  8. doi request reprint Hepcidin in human iron disorders: diagnostic implications
    Joyce J C Kroot
    Department of Laboratory Medicine, Laboratory of Genetic, Endocrine and Metabolic Disorders, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Chem 57:1650-69. 2011
    ..The measurement of hepcidin in biological fluids is therefore a promising tool in the diagnosis and management of medical conditions in which iron metabolism is affected...
  9. pmc Surface-exposed histone-like protein a modulates adherence of Streptococcus gallolyticus to colon adenocarcinoma cells
    Annemarie Boleij
    Department of Laboratory Medicine, Nijmegen Institutes for Infection, Inflammation and Immunity and Oncology of the Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands
    Infect Immun 77:5519-27. 2009
    ..This may be explained by the fact that heparan sulfate and LTA compete for the same binding site in HlpA. Altogether, this study implies that HlpA serves as a fine-tuning factor for bacterial adherence...
  10. pmc Increased exposure to bacterial antigen RpL7/L12 in early stage colorectal cancer patients
    Annemarie Boleij
    Department of Laboratory Medicine Clinical Chemistry, Nijmegen Institute for Infection, Inflammation, and Immunity, Radboud University Center for Oncology of the Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Cancer 116:4014-22. 2010
    ..This work aims to investigate the humoral immune response to this bacterium during different stages of colorectal cancer...
  11. ncbi request reprint Regulation of hepcidin: insights from biochemical analyses on human serum samples
    Erwin H J M Kemna
    Department of Clinical Chemistry 441, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Blood Cells Mol Dis 40:339-46. 2008
    ..Future studies are needed to validate the combined utility of this predictive algorithm together with actual measured hepcidin levels in clinical diagnosis...
  12. doi request reprint Immunochemical and mass-spectrometry-based serum hepcidin assays for iron metabolism disorders
    Joyce J C Kroot
    Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Chem 56:1570-9. 2010
    ..Hepcidin is instrumental in the diagnosis and monitoring of iron metabolism disorders, but reliable methods for its quantification in serum are sparse, as is knowledge of their relative analytical strengths and clinical utility...
  13. doi request reprint Second round robin for plasma hepcidin methods: first steps toward harmonization
    Joyce J C Kroot
    Department of Laboratory Medicine, Laboratory of Genetic Endocrine and Metabolic Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Am J Hematol 87:977-83. 2012
    ..This study represents the first step toward harmonization of plasma hepcidin methods and facilitates aggregation of hepcidin data from different research investigations...
  14. pmc Mass spectrometry analysis of hepcidin peptides in experimental mouse models
    Harold Tjalsma
    Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    PLoS ONE 6:e16762. 2011
    ....
  15. doi request reprint Novel clues on the specific association of Streptococcus gallolyticus subsp gallolyticus with colorectal cancer
    Annemarie Boleij
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    J Infect Dis 203:1101-9. 2011
    ..Therefore, this study aimed to reveal the virulence characteristics that distinguish this opportunistic pathogen from a panel of (closely related) intestinal bacteria...
  16. pmc Surface-affinity profiling to identify host-pathogen interactions
    Annemarie Boleij
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity and Radboud University Centrefor Oncology of the Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Infect Immun 79:4777-83. 2011
    ..In conclusion, our study shows that surface-affinity profiling is a valuable tool to identify novel adhesin-receptor pairs, which advocates its application in other hybrid biological systems...
  17. doi request reprint (Pre)analytical imprecision, between-subject variability, and daily variations in serum and urine hepcidin: implications for clinical studies
    Joyce J C Kroot
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Anal Biochem 389:124-9. 2009
    ..Transferrin saturation did not correlate with hepcidin levels. To minimize variability, we recommend (i) standardizing for sampling time and (ii) measuring serum hepcidin levels...
  18. pmc Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry
    Dorine W Swinkels
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    PLoS ONE 3:e2706. 2008
    ....
  19. ncbi request reprint The itinerary of Streptococcus gallolyticus infection in patients with colonic malignant disease
    Annemarie Boleij
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i and Radboud University Centre for Oncology RUCO, Radboud University Medical Centre, Nijmegen, The Netherlands
    Lancet Infect Dis 13:719-24. 2013
    ..We believe that our current reconstruction of this route of infection aids understanding of how S. gallolyticus gallolyticus infections can be best exploited for early detection of colorectal cancer...
  20. pmc Selective antibody response to Streptococcus gallolyticus pilus proteins in colorectal cancer patients
    Annemarie Boleij
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i, Nijmegen, The Netherlands
    Cancer Prev Res (Phila) 5:260-5. 2012
    ..Together these findings underscore the potential of a multi-antigen approach to complement diagnosis of S. gallolyticus subsp. gallolyticus-associated CRC...
  21. ncbi request reprint High-sensitive radioimmunoassay for human serum hepcidin
    Nicolai Grebenchtchikov
    Department of Chemical Endocrinology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Br J Haematol 146:317-25. 2009
    ....
  22. doi request reprint Hepcidin: from discovery to differential diagnosis
    Erwin H J M Kemna
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, P O Box 9101 6500 HB Nijmegen, The Netherlands
    Haematologica 93:90-7. 2008
    ..Ongoing studies in humans should provide us with more information on the etiology of iron metabolism disorders in order to create new therapeutic strategies and improve differential diagnosis protocols for these diseases...
  23. ncbi request reprint Nitric oxide does not contribute to inflammation-induced hypoferremia in humans
    Erwin H J M Kemna
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Acta Haematol 118:149-52. 2007
  24. doi request reprint Screening metatranscriptomes for toxin genes as functional drivers of human colorectal cancer
    Bas E Dutilh
    Centre for Molecular and Biomolecular Informatics, Radboud University Medical Centre, Geert Grooteplein 28, 6525 GA Nijmegen, The Netherlands
    Best Pract Res Clin Gastroenterol 27:85-99. 2013
    ..Thus, extended metagenomic studies are needed to obtain a high-resolution picture of host-pathogen interactions during human CRC...
  25. doi request reprint Shedding & shaving: disclosure of proteomic expressions on a bacterial face
    Harold Tjalsma
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Proteomics 8:1415-28. 2008
    ..Together this shows that these combined gel-free approaches can reveal novel proteomic decorations on a bacterial face and may provide leads for future studies on protein sorting in B. subtilis and other Gram-positive bacteria...
  26. pmc Improved mass spectrometry assay for plasma hepcidin: detection and characterization of a novel hepcidin isoform
    Coby M M Laarakkers
    Laboratory of Genetic, Endocrine and Metabolic Diseases, Department of Laboratory Medicine, Radboud University Medical Centre, Nijmegen, The Netherlands Hepcidinanalysis com, Nijmegen, The Netherlands
    PLoS ONE 8:e75518. 2013
    ....
  27. ncbi request reprint Proteomic inventory of "anchorless" proteins on the colon adenocarcinoma cell surface
    Harold Tjalsma
    Department of Clinical Chemistry, 441, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Biochim Biophys Acta 1764:1607-17. 2006
    ....
  28. ncbi request reprint Novel urine hepcidin assay by mass spectrometry
    Erwin Kemna
    Department of Clinical Chemistry 564, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    Blood 106:3268-70. 2005
    ..In conclusion, this novel MS urine hepcidin assay is easy to perform and available to a wide audience. This enables the implementation of hepcidin measurements in large clinical studies...
  29. ncbi request reprint Feature-based reappraisal of the Bacillus subtilis exoproteome
    Harold Tjalsma
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Proteomics 7:73-81. 2007
    ..Finally, the present study suggests that feature-based outlier analysis may provide leads towards the discovery of novel special-purpose pathways for bacterial protein export...
  30. ncbi request reprint Simultaneous proteomic and genomic analysis of primary Ta urothelial cell carcinomas for the prediction of tumor recurrence
    Iman J Schultz
    Departments of Clinical Chemistry, Radboud University Nijmegen Medical Center, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    Anticancer Res 27:1051-8. 2007
    ..The prediction of tumor recurrence in patients with Ta urothelial cell carcinoma is inaccurate and new prognostic markers are desirable...
  31. pmc Iron-induced virulence of Salmonella enterica serovar typhimurium at the intestinal epithelial interface can be suppressed by carvacrol
    Guus A M Kortman
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation, and Immunity N4i, and Institute for Genetic and Metabolic Disease IGMD of the Radboud University Medical Center, Nijmegen, The Netherlands
    Antimicrob Agents Chemother 58:1664-70. 2014
    ..In conclusion, our in vitro data suggest that carvacrol has the potential to serve as a novel dietary supplement to prevent pathogenic overgrowth and colonization in the large intestine during oral iron therapy. ..
  32. doi request reprint Gut bacteria in health and disease: a survey on the interface between intestinal microbiology and colorectal cancer
    Annemarie Boleij
    Department of Laboratory Medicine, Nijmegen Institute for Infection, Inflammation and Immunity N4i and Radboud University Centre for Oncology RUCO of the Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Biol Rev Camb Philos Soc 87:701-30. 2012
    ..A thorough understanding of these processes will provide leads for future research and may ultimately aid in development of new strategies for CRC diagnosis and prevention...
  33. doi request reprint Immunoproteomics: From biomarker discovery to diagnostic applications
    Harold Tjalsma
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Proteomics Clin Appl 2:167-80. 2008
    ....
  34. ncbi request reprint Profiling the humoral immune response in colon cancer patients: diagnostic antigens from Streptococcus bovis
    Harold Tjalsma
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Int J Cancer 119:2127-35. 2006
    ..This makes profiling of the humoral immune response against "silent" S. bovis infections a promising diagnostic tool for the early detection of human colon cancer, which is crucial for the effective treatment of this disease...
  35. ncbi request reprint Mass spectrometry-based hepcidin measurements in serum and urine: analytical aspects and clinical implications
    Erwin H J M Kemna
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Clin Chem 53:620-8. 2007
    ..Recent reports on the measurement of urine and serum hepcidin by surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) necessitate analytical and clinical evaluation of MS-based methodologies...
  36. ncbi request reprint Proteomics-based consensus prediction of protein retention in a bacterial membrane
    Harold Tjalsma
    Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, The Netherlands
    Proteomics 5:4472-82. 2005
    ..Our findings provide important leads for future identification of surface proteins from pathogenic bacteria, which are attractive candidate infection markers and potential targets for drugs or vaccines...