R J Siezen

Summary

Affiliation: NIZO Food Research
Country: The Netherlands

Publications

  1. pmc Subtilases: the superfamily of subtilisin-like serine proteases
    R J Siezen
    Department of Biophysical Chemistry, NIZO, Ede, The Netherlands
    Protein Sci 6:501-23. 1997
  2. ncbi request reprint Multi-domain, cell-envelope proteinases of lactic acid bacteria
    R J Siezen
    NIZO Food Research, Ede, The Netherlands
    Antonie Van Leeuwenhoek 76:139-55. 1999
  3. ncbi request reprint Engineering of the Lactococcus lactis serine proteinase by construction of hybrid enzymes
    P Vos
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO, Ede
    Protein Eng 4:479-84. 1991
  4. ncbi request reprint Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363
    M van Asseldonk
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research, NIZO, Ede
    Gene 95:155-60. 1990
  5. ncbi request reprint Pore formation by nisin involves translocation of its C-terminal part across the membrane
    C van Kraaij
    Microbial Ingredients Section, NIZO Food Research, Ede, The Netherlands
    Biochemistry 37:16033-40. 1998
  6. ncbi request reprint Overproduction of bovine beta-casein in Escherichia coli and engineering of its main chymosin cleavage site
    G Simons
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research NIZO, Ede, The Netherlands
    Protein Eng 6:763-70. 1993
  7. pmc Cloning, characterization, controlled overexpression, and inactivation of the major tributyrin esterase gene of Lactococcus lactis
    L Fernandez
    NIZO Food Research, Ede, The Netherlands
    Appl Environ Microbiol 66:1360-8. 2000
  8. ncbi request reprint Primary structure and organization of the gene for a procaryotic, cell envelope-located serine proteinase
    P Vos
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research NIZO, Ede
    J Biol Chem 264:13579-85. 1989
  9. ncbi request reprint Homology modelling of the catalytic domain of human furin. A model for the eukaryotic subtilisin-like proprotein convertases
    R J Siezen
    Department of Biophysical Chemistry, NIZO, Ede, The Netherlands
    Eur J Biochem 222:255-66. 1994
  10. ncbi request reprint Metabolic models for rational improvement of lactic acid bacteria as cell factories
    E J Smid
    Wageningen Centre for Food Sciences, Wageningen, The Netherlands
    J Appl Microbiol 98:1326-31. 2005

Collaborators

Detail Information

Publications13

  1. pmc Subtilases: the superfamily of subtilisin-like serine proteases
    R J Siezen
    Department of Biophysical Chemistry, NIZO, Ede, The Netherlands
    Protein Sci 6:501-23. 1997
    ..Predictions have been updated for Ca(2+)-binding sites, disulfide bonds, and substrate specificity, based on both sequence alignment and three-dimensional homology modeling...
  2. ncbi request reprint Multi-domain, cell-envelope proteinases of lactic acid bacteria
    R J Siezen
    NIZO Food Research, Ede, The Netherlands
    Antonie Van Leeuwenhoek 76:139-55. 1999
    ..Not all domains are present in each cell-envelope proteinase, suggesting that these multi-domain proteins are the result of gene shuffling and domain swapping during evolution...
  3. ncbi request reprint Engineering of the Lactococcus lactis serine proteinase by construction of hybrid enzymes
    P Vos
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO, Ede
    Protein Eng 4:479-84. 1991
    ..This suggests that the 200 kd L. lactis proteinase may be the representative of a new subclass of subtilisin-like enzymes...
  4. ncbi request reprint Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363
    M van Asseldonk
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research, NIZO, Ede
    Gene 95:155-60. 1990
    ..Usp45 has an unusual aa composition and distribution, and it is predicted to be structurally homologous with P54 of Enterococcus faecium. Up to now, no biological activity could be postulated for this secreted protein...
  5. ncbi request reprint Pore formation by nisin involves translocation of its C-terminal part across the membrane
    C van Kraaij
    Microbial Ingredients Section, NIZO Food Research, Ede, The Netherlands
    Biochemistry 37:16033-40. 1998
    ..The results demonstrate for the first time that pore formation of nisin involves translocation of the C-terminal region of the molecule across the membrane...
  6. ncbi request reprint Overproduction of bovine beta-casein in Escherichia coli and engineering of its main chymosin cleavage site
    G Simons
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research NIZO, Ede, The Netherlands
    Protein Eng 6:763-70. 1993
    ..The beta-casein cDNA was altered to change the main chymosin cleavage site in beta-casein at position 192-193 in two ways, namely from Leu-Tyr to Pro-Pro and to Leu-stop.(ABSTRACT TRUNCATED AT 250 WORDS)..
  7. pmc Cloning, characterization, controlled overexpression, and inactivation of the major tributyrin esterase gene of Lactococcus lactis
    L Fernandez
    NIZO Food Research, Ede, The Netherlands
    Appl Environ Microbiol 66:1360-8. 2000
    ....
  8. ncbi request reprint Primary structure and organization of the gene for a procaryotic, cell envelope-located serine proteinase
    P Vos
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research NIZO, Ede
    J Biol Chem 264:13579-85. 1989
    ..Furthermore, a region was identified in the Lactococcus proteinase, which shows homology to the membrane-anchoring domains of a number of proteins from other Gram-positive bacteria...
  9. ncbi request reprint Homology modelling of the catalytic domain of human furin. A model for the eukaryotic subtilisin-like proprotein convertases
    R J Siezen
    Department of Biophysical Chemistry, NIZO, Ede, The Netherlands
    Eur J Biochem 222:255-66. 1994
    ....
  10. ncbi request reprint Metabolic models for rational improvement of lactic acid bacteria as cell factories
    E J Smid
    Wageningen Centre for Food Sciences, Wageningen, The Netherlands
    J Appl Microbiol 98:1326-31. 2005
  11. ncbi request reprint Isolation and characterization of the hyperthermostable serine protease, pyrolysin, and its gene from the hyperthermophilic archaeon Pyrococcus furiosus
    W G Voorhorst
    Department of Microbiology, Wageningen Agricultural University, 6703 CT Wageningen
    J Biol Chem 271:20426-31. 1996
    ....
  12. ncbi request reprint Purification, characterization, and molecular modeling of pyrolysin and other extracellular thermostable serine proteases from hyperthermophilic microorganisms
    W M de Vos
    Laboratory of Microbiology, Wageningen Agricultural University, Wageningen, NL 6703 CT, The Netherlands
    Methods Enzymol 330:383-93. 2001
  13. ncbi request reprint Lactic acid bacteria: genetics, metabolism and applications
    R J Siezen
    Antonie Van Leeuwenhoek 82:1. 2002