Ruben van Boxtel

Summary

Affiliation: Netherlands Institute of Ecology
Country: The Netherlands

Publications

  1. pmc Improved generation of rat gene knockouts by target-selected mutagenesis in mismatch repair-deficient animals
    Ruben van Boxtel
    Hubrecht Institute for Developmental Biology and Stem Cell Research, Cancer Genomics Center, Royal Netherlands Academy of Sciences, Utrecht, The Netherlands
    BMC Genomics 9:460. 2008
  2. doi request reprint Lack of DNA mismatch repair protein MSH6 in the rat results in hereditary non-polyposis colorectal cancer-like tumorigenesis
    Ruben van Boxtel
    Hubrecht Institute for Developmental Biology and Stem Cell Research, Cancer Genomics Center, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Carcinogenesis 29:1290-7. 2008
  3. doi request reprint ENU mutagenesis to generate genetically modified rat models
    Ruben van Boxtel
    Hubrect Institute, NIOB KNAW, Utrecht, The Netherlands
    Methods Mol Biol 597:151-67. 2010
  4. pmc Genome-wide analysis of FOXO3 mediated transcription regulation through RNA polymerase II profiling
    Astrid Eijkelenboom
    Department of Molecular Cancer Research, University Medical Centre, Utrecht, The Netherlands
    Mol Syst Biol 9:638. 2013
  5. pmc Homozygous and heterozygous p53 knockout rats develop metastasizing sarcomas with high frequency
    Ruben van Boxtel
    Hubrecht Institute for Developmental Biology and Stem Cell Research, Cancer Genomics Center, KNAW and University Medical Center Utrecht, Utrecht, The Netherlands
    Am J Pathol 179:1616-22. 2011
  6. doi request reprint Generation of genetically modified rodents using random ENU mutagenesis
    Ruben van Boxtel
    Royal Netherlands Academy of Sciences, Hubrecht Institute and University Medical Center Utrecht, Utrecht, The Netherlands
    Methods Mol Biol 693:295-308. 2011
  7. doi request reprint Mutation discovery by targeted genomic enrichment of multiplexed barcoded samples
    Isaac J Nijman
    Hubrecht Institute, Developmental Biology and Stem Cell Research, Royal Netherlands Academy of Arts and Sciences and the University Medical Center Utrecht, Utrecht, The Netherlands
    Nat Methods 7:913-5. 2010
  8. pmc Rat traps: filling the toolbox for manipulating the rat genome
    Ruben van Boxtel
    Hubrecht Institute for Developmental Biology and Stem Cell Research, Cancer Genomics Center, Royal Netherlands Academy of Sciences and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Genome Biol 11:217. 2010
  9. pmc Melanocortin receptor 4 deficiency affects body weight regulation, grooming behavior, and substrate preference in the rat
    Joram D Mul
    Hubrecht Institute KNAW and University Medical Center Utrecht, Utrecht, The Netherlands
    Obesity (Silver Spring) 20:612-21. 2012
  10. pmc SOX4 mediates TGF-β-induced expression of mesenchymal markers during mammary cell epithelial to mesenchymal transition
    Stephin J Vervoort
    Department of Cell Biology, University Medical Centre, Utrecht, The Netherlands
    PLoS ONE 8:e53238. 2013

Collaborators

Detail Information

Publications10

  1. pmc Improved generation of rat gene knockouts by target-selected mutagenesis in mismatch repair-deficient animals
    Ruben van Boxtel
    Hubrecht Institute for Developmental Biology and Stem Cell Research, Cancer Genomics Center, Royal Netherlands Academy of Sciences, Utrecht, The Netherlands
    BMC Genomics 9:460. 2008
    ..N-ethyl-N-nitrosourea (ENU)-driven target-selected mutagenesis is currently the most successful method in rats, although it is still very laborious and expensive...
  2. doi request reprint Lack of DNA mismatch repair protein MSH6 in the rat results in hereditary non-polyposis colorectal cancer-like tumorigenesis
    Ruben van Boxtel
    Hubrecht Institute for Developmental Biology and Stem Cell Research, Cancer Genomics Center, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Carcinogenesis 29:1290-7. 2008
    ..The MSH6 knockout rat complements existing models for studying genetic instable tumorigenesis as it provides experimental opportunities that are not available or suboptimal in current models...
  3. doi request reprint ENU mutagenesis to generate genetically modified rat models
    Ruben van Boxtel
    Hubrect Institute, NIOB KNAW, Utrecht, The Netherlands
    Methods Mol Biol 597:151-67. 2010
    ..Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism...
  4. pmc Genome-wide analysis of FOXO3 mediated transcription regulation through RNA polymerase II profiling
    Astrid Eijkelenboom
    Department of Molecular Cancer Research, University Medical Centre, Utrecht, The Netherlands
    Mol Syst Biol 9:638. 2013
    ..Combined, our data elucidate how FOXOs regulate gene transcription and provide insight into mechanisms by which FOXOs can induce different gene expression programs depending on chromatin architecture...
  5. pmc Homozygous and heterozygous p53 knockout rats develop metastasizing sarcomas with high frequency
    Ruben van Boxtel
    Hubrecht Institute for Developmental Biology and Stem Cell Research, Cancer Genomics Center, KNAW and University Medical Center Utrecht, Utrecht, The Netherlands
    Am J Pathol 179:1616-22. 2011
    ..These unique features make this rat highly complementary to other rodent p53 knockout models and a versatile tool for investigating tumorigenesis processes as well as genotoxic studies...
  6. doi request reprint Generation of genetically modified rodents using random ENU mutagenesis
    Ruben van Boxtel
    Royal Netherlands Academy of Sciences, Hubrecht Institute and University Medical Center Utrecht, Utrecht, The Netherlands
    Methods Mol Biol 693:295-308. 2011
    ..Here, we describe the detailed protocols for ENU mutagenesis and for mutant retrieval...
  7. doi request reprint Mutation discovery by targeted genomic enrichment of multiplexed barcoded samples
    Isaac J Nijman
    Hubrecht Institute, Developmental Biology and Stem Cell Research, Royal Netherlands Academy of Arts and Sciences and the University Medical Center Utrecht, Utrecht, The Netherlands
    Nat Methods 7:913-5. 2010
    ..We screened 770 genes (1.4 megabases) in thirty N-ethyl-N-nitrosourea (ENU)-mutagenized rats and identified known variants at >96% sensitivity as well as new mutations at a false positive rate < 1 in 8 megabases...
  8. pmc Rat traps: filling the toolbox for manipulating the rat genome
    Ruben van Boxtel
    Hubrecht Institute for Developmental Biology and Stem Cell Research, Cancer Genomics Center, Royal Netherlands Academy of Sciences and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Genome Biol 11:217. 2010
    ..Here we outline the current status, possibilities and application of these techniques in the rat...
  9. pmc Melanocortin receptor 4 deficiency affects body weight regulation, grooming behavior, and substrate preference in the rat
    Joram D Mul
    Hubrecht Institute KNAW and University Medical Center Utrecht, Utrecht, The Netherlands
    Obesity (Silver Spring) 20:612-21. 2012
    ....
  10. pmc SOX4 mediates TGF-β-induced expression of mesenchymal markers during mammary cell epithelial to mesenchymal transition
    Stephin J Vervoort
    Department of Cell Biology, University Medical Centre, Utrecht, The Netherlands
    PLoS ONE 8:e53238. 2013
    ..Taken together, these data suggest that TGF-b-mediated increased expression of SOX4 is required for the induction of a mesenchymal phenotype during EMT in human mammary epithelial cells...