R H Plasterk

Summary

Affiliation: Netherlands Institute of Ecology
Country: The Netherlands

Publications

  1. pmc Cloning and expression of new microRNAs from zebrafish
    Wigard P Kloosterman
    Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nucleic Acids Res 34:2558-69. 2006
  2. pmc RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans
    Bastiaan B J Tops
    Hubrecht Laboratory, Centre for Biomedical Genetics Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nucleic Acids Res 33:347-55. 2005
  3. pmc Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions
    Femke Simmer
    Hubrecht Laboratory, Centre for Biomedical Genetics, Utrecht, The Netherlands
    PLoS Biol 1:E12. 2003
  4. pmc MicroRNAs show a wide diversity of expression profiles in the developing and mature central nervous system
    Marika Kapsimali
    Department of Anatomy and Developmental Biology, UCL, Gower Street, London WC1E 6BT, UK
    Genome Biol 8:R173. 2007
  5. pmc Generation of medaka gene knockout models by target-selected mutagenesis
    Yoshihito Taniguchi
    Department of Radiation Genetics, CREST, Japan Science and Technology Laboratory, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Genome Biol 7:R116. 2006
  6. ncbi request reprint Micro RNAs in animal development
    Ronald H A Plasterk
    Hubrecht Laboratory KNAW, Utrecht University, 3584CT Utrecht, The Netherlands
    Cell 124:877-81. 2006
  7. ncbi request reprint The silence of the genes
    R H Plasterk
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT, The, Utrecht, Netherlands
    Curr Opin Genet Dev 10:562-7. 2000
  8. ncbi request reprint RNA silencing: the genome's immune system
    Ronald H A Plasterk
    Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, Netherlands
    Science 296:1263-5. 2002
  9. pmc Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
    R F Ketting
    The Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, Utrecht, The Netherlands
    Genes Dev 15:2654-9. 2001
  10. ncbi request reprint On the role of RNA amplification in dsRNA-triggered gene silencing
    T Sijen
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Cell 107:465-76. 2001

Collaborators

Detail Information

Publications70

  1. pmc Cloning and expression of new microRNAs from zebrafish
    Wigard P Kloosterman
    Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nucleic Acids Res 34:2558-69. 2006
    ..Most newly discovered miRNAs have low expression levels and are less conserved in other vertebrate species. Our cloning and expression analysis indicates that most abundant and conserved miRNAs in zebrafish are now known...
  2. pmc RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans
    Bastiaan B J Tops
    Hubrecht Laboratory, Centre for Biomedical Genetics Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nucleic Acids Res 33:347-55. 2005
    ..Together these data support a role for the MUT-7/RDE-2 complex downstream of siRNA formation, but upstream of siRNA mediated target RNA recognition, possibly indicating a role in the siRNA amplification step...
  3. pmc Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions
    Femke Simmer
    Hubrecht Laboratory, Centre for Biomedical Genetics, Utrecht, The Netherlands
    PLoS Biol 1:E12. 2003
    ..elegans genome. The resulting dataset will be valuable in conjunction with other functional genomics approaches, as well as in other model organisms...
  4. pmc MicroRNAs show a wide diversity of expression profiles in the developing and mature central nervous system
    Marika Kapsimali
    Department of Anatomy and Developmental Biology, UCL, Gower Street, London WC1E 6BT, UK
    Genome Biol 8:R173. 2007
    ..To provide an overview of the breadth of miRNA expression in the CNS, we performed a comprehensive analysis of the neuroanatomical expression profiles of 38 abundant conserved miRNAs in developing and adult zebrafish brain...
  5. pmc Generation of medaka gene knockout models by target-selected mutagenesis
    Yoshihito Taniguchi
    Department of Radiation Genetics, CREST, Japan Science and Technology Laboratory, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Genome Biol 7:R116. 2006
    ..The current cryopreserved resource is expected to contain knockouts for most medaka genes...
  6. ncbi request reprint Micro RNAs in animal development
    Ronald H A Plasterk
    Hubrecht Laboratory KNAW, Utrecht University, 3584CT Utrecht, The Netherlands
    Cell 124:877-81. 2006
    ..This Essay explores the importance of miRNAs in animal development and their possible roles in disease and evolution...
  7. ncbi request reprint The silence of the genes
    R H Plasterk
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT, The, Utrecht, Netherlands
    Curr Opin Genet Dev 10:562-7. 2000
    ....
  8. ncbi request reprint RNA silencing: the genome's immune system
    Ronald H A Plasterk
    Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, Netherlands
    Science 296:1263-5. 2002
    ..The latter property is beginning to be understood at the molecular level...
  9. pmc Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
    R F Ketting
    The Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, Utrecht, The Netherlands
    Genes Dev 15:2654-9. 2001
    ..dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41)...
  10. ncbi request reprint On the role of RNA amplification in dsRNA-triggered gene silencing
    T Sijen
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Cell 107:465-76. 2001
    ..This amplification mechanism substantially augments the potency of RNAi-based surveillance, while ensuring that the RNAi machinery will focus on expressed mRNAs...
  11. ncbi request reprint Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map
    S R Wicks
    The Hubrecht Laboratory and Center for Biomedical Genetics. Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Nat Genet 28:160-4. 2001
    ..The usefulness of this approach is illustrated by the rapid mapping of the dyf-5 gene...
  12. pmc The G-protein beta-subunit GPB-2 in Caenorhabditis elegans regulates the G(o)alpha-G(q)alpha signaling network through interactions with the regulator of G-protein signaling proteins EGL-10 and EAT-16
    A M Van Der Linden
    Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Genetics 158:221-35. 2001
    ..Taken together, our results suggest that GPB-2 works in concert with the RGS proteins EGL-10 and EAT-16 to regulate GOA-1 (G(o)alpha) and EGL-30 (G(q)alpha) signaling...
  13. pmc Regulated transposition of a fish transposon in the mouse germ line
    S E Fischer
    Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 98:6759-64. 2001
    ..Analysis of the excision sites suggests that double-strand breaks in haploid spermatids are repaired via nonhomologous end joining. The SB system may be a powerful tool for transposon mutagenesis of the mouse germ line...
  14. ncbi request reprint Gene expression: long-term gene silencing by RNAi
    Nadine L Vastenhouw
    Hubrecht Laboratory KNAW, University of Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nature 442:882. 2006
    ..Our findings may prove useful in the ongoing development of RNAi to treat disease...
  15. ncbi request reprint Identification of conserved pathways of DNA-damage response and radiation protection by genome-wide RNAi
    Gijs van Haaften
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Curr Biol 16:1344-50. 2006
    ....
  16. ncbi request reprint Target-selected gene inactivation in zebrafish
    Erno Wienholds
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Methods Cell Biol 77:69-90. 2004
  17. pmc Chromatin and RNAi factors protect the C. elegans germline against repetitive sequences
    Valérie J P Robert
    Hubrecht Laboratory, 3584 CT Utrecht, The Netherlands
    Genes Dev 19:782-7. 2005
    ..Together with molecular data also presented in this study, these results suggest that in C. elegans repetitive sequences trigger transcriptional gene silencing using RNAi and chromatin factors...
  18. ncbi request reprint In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes
    Wigard P Kloosterman
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nat Methods 3:27-9. 2006
    ..Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo...
  19. ncbi request reprint Camels and zebrafish, viruses and cancer: a microRNA update
    Eugene Berezikov
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Hum Mol Genet 14:R183-90. 2005
    ....
  20. ncbi request reprint MicroRNA function in animal development
    Erno Wienholds
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    FEBS Lett 579:5911-22. 2005
    ..Here, we review the recent advances in miRNA biology in animals. Particularly, we focus on the roles of miRNAs in vertebrate development and disease...
  21. ncbi request reprint MicroRNA expression in zebrafish embryonic development
    Erno Wienholds
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Science 309:310-1. 2005
    ....
  22. pmc Differences in vertebrate microRNA expression
    Brandon Ason
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 103:14385-9. 2006
    ....
  23. pmc Mouse microRNA profiles determined with a new and sensitive cloning method
    Shuji Takada
    Division of Functional Genomics, Jichi Medical University, 3311 1 Yakushiji, Shimotsukeshi, Tochigi 329 0498, Japan
    Nucleic Acids Res 34:e115. 2006
    ..Our data thus indicate that the total number of miRNAs in vertebrates is larger than previously appreciated and that the expression of these molecules is tightly controlled in a tissue- and developmental stage-specific manner...
  24. pmc C. elegans model identifies genetic modifiers of alpha-synuclein inclusion formation during aging
    Tjakko J van Ham
    Department of Genetics, University Medical Centre Groningen and University of Groningen, Groningen, The Netherlands
    PLoS Genet 4:e1000027. 2008
    ..The processes and genes identified here present a framework for further study of the disease mechanism and provide candidate susceptibility genes and drug targets for Parkinson's disease and other alpha-synuclein related disorders...
  25. pmc RAP-1 and the RAL-1/exocyst pathway coordinate hypodermal cell organization in Caenorhabditis elegans
    Ester W Frische
    Department of Physiological Chemistry, Centre for Biomedical Genetics, UMC Utrecht, Utrecht, The Netherlands
    EMBO J 26:5083-92. 2007
    ..Genetic interactions show that the RAP-1 and RAL-1/exocyst pathway also act in parallel during larval stages. Together these data provide in vivo evidence for the exocyst complex as a downstream RAL-1 effector in cell migration...
  26. ncbi request reprint Structural features of small RNA precursors determine Argonaute loading in Caenorhabditis elegans
    Florian A Steiner
    Hubrecht Institute, Koninklijke Nederlandse Akademie van Wetenschappen, Utrecht, 3584 CT, The Netherlands
    Nat Struct Mol Biol 14:927-33. 2007
    ..The Argonaute proteins to which the small RNAs are bound determine the silencing mode, and no functional overlap between RDE-1 and ALG-1 was detected...
  27. pmc Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development
    Wigard P Kloosterman
    Hubrecht Laboratory KNAW, Utrecht, The Netherlands
    PLoS Biol 5:e203. 2007
    ..The miRNA knockdown strategy presented here will be widely used to unravel miRNA function in zebrafish...
  28. pmc Mechanistic insights and identification of two novel factors in the C. elegans NMD pathway
    Dasa Longman
    Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland, United Kingdom
    Genes Dev 21:1075-85. 2007
    ..elegans that we have termed smgl (for smg lethal). We show that the encoded proteins are conserved throughout evolution and are required for NMD in C. elegans and also in human cells...
  29. ncbi request reprint A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish
    Saskia Houwing
    Hubrecht Laboratory, Uppsalalaan 8, Utrecht, Netherlands
    Cell 129:69-82. 2007
    ..Furthermore, we show that piRNAs are Dicer independent and that their 3' end likely carries a 2'O-Methyl modification...
  30. pmc Efficient target-selected mutagenesis in Caenorhabditis elegans: toward a knockout for every gene
    Edwin Cuppen
    Hubrecht Laboratory, 3584 CT Utrecht, The Netherlands
    Genome Res 17:649-58. 2007
    ..For EMS-mutagenized C. elegans, there are only approximately 500,000 NIMs. We show that a NIM genotyping approach employing high-density microarrays can, in principle, be used for the genome-wide identification of C. elegans knockouts...
  31. pmc A Caenorhabditis elegans wild type defies the temperature-size rule owing to a single nucleotide polymorphism in tra-3
    Jan E Kammenga
    Laboratory of Nematology, Wageningen University, Wageningen, The Netherlands
    PLoS Genet 3:e34. 2007
    ..These findings provide a novel step toward the molecular understanding of the temperature-size rule, which has puzzled biologists for decades...
  32. ncbi request reprint Secondary siRNAs result from unprimed RNA synthesis and form a distinct class
    Titia Sijen
    Hubrecht Laboratory NIOB KNAW, Uppsalalaan 8, 3584 CT, The Netherlands
    Science 315:244-7. 2007
    ..Therefore, secondary siRNAs represent a distinct class of small RNAs. Their biogenesis depends on RdRPs, and we propose that each secondary siRNA is an individual RdRP product...
  33. ncbi request reprint Diversity of microRNAs in human and chimpanzee brain
    Eugene Berezikov
    Hubrecht Laboratory KNAW, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nat Genet 38:1375-7. 2006
    ..These data suggest that evolution of miRNAs is an ongoing process and that along with ancient, highly conserved miRNAs, there are a number of emerging miRNAs...
  34. pmc Substrate requirements for let-7 function in the developing zebrafish embryo
    Wigard P Kloosterman
    The Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Nucleic Acids Res 32:6284-91. 2004
    ..We took advantage of reporter and phenotypic assays to analyze the activity of all possible point mutant derivatives of let-7 and found that only the 5' region is critical for function of let-7...
  35. ncbi request reprint Processing of primary microRNAs by the Microprocessor complex
    Ahmet M Denli
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 432:231-5. 2004
    ..Considered together, these results indicate a role for Pasha in miRNA maturation and miRNA-mediated gene regulation...
  36. ncbi request reprint Genomic instability and cancer: scanning the Caenorhabditis elegans genome for tumor suppressors
    Gijs van Haaften
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, Utrecht 3584 CT, The Netherlands
    Oncogene 23:8366-75. 2004
    ..Here, we discuss genomic approaches in the model organism Caenorhabditis elegans, aimed to identify genes and genetic networks that contribute to genome stability and are thus potentially involved in human carcinogenesis...
  37. ncbi request reprint A micrococcal nuclease homologue in RNAi effector complexes
    Amy A Caudy
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 425:411-4. 2003
    ..Tudor-SN is the first RISC subunit to be identified that contains a recognizable nuclease domain, and could therefore contribute to the RNA degradation observed in RNAi...
  38. ncbi request reprint A position-dependent organisation of retinoid response elements is conserved in the vertebrate Hox clusters
    Gaell Mainguy
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Trends Genet 19:476-9. 2003
  39. ncbi request reprint A genome-wide screen identifies 27 genes involved in transposon silencing in C. elegans
    Nadine L Vastenhouw
    Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Curr Biol 13:1311-6. 2003
    ..Since most of the newly identified genes have clear homologs in other species, and since transposons are found from protozoa to human, it seems likely that they also protect other genomes against transposon activity in the germline...
  40. ncbi request reprint Metabolic labeling of C. elegans and D. melanogaster for quantitative proteomics
    Jeroen Krijgsveld
    Center for Biomedical Genetics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    Nat Biotechnol 21:927-31. 2003
    ..elegans strains, one with and one without a germ line. The methodology described provides tools for accurate quantitative proteomic studies in these model organisms...
  41. pmc Continuous exchange of sequence information between dispersed Tc1 transposons in the Caenorhabditis elegans genome
    Sylvia E J Fischer
    Hubrecht Laboratory, Center for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Genetics 164:127-34. 2003
    ..elegans is highly dynamic because of a continuous exchange of sequence information between individual copies, potentially allowing a higher evolution rate than that found in endogenous genes...
  42. ncbi request reprint Hyperactivation of the G12-mediated signaling pathway in Caenorhabditis elegans induces a developmental growth arrest via protein kinase C
    Alexander M van der Linden
    Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Curr Biol 13:516-21. 2003
    ..elegans. Taken together, our findings provide a potential molecular mechanism for the transforming capability of G(12) proteins...
  43. pmc Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi
    Joris Pothof
    Hubrecht Laboratory, Center for Biomedical Genetics, 3584 CT, Utrecht, The Netherlands
    Genes Dev 17:443-8. 2003
    ..Because known mutator genes are causally involved in many hereditary and sporadic human cancers, it is likely that some of these new mutators are equally relevant in cancer etiology...
  44. ncbi request reprint The genetics of RNA silencing
    Marcel Tijsterman
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Annu Rev Genet 36:489-519. 2002
    ..This chapter gives an overview of both biochemical and genetic approaches leading to the current understanding of the molecular mechanism of RNA silencing and its probable biological function...
  45. ncbi request reprint PPW-1, a PAZ/PIWI protein required for efficient germline RNAi, is defective in a natural isolate of C. elegans
    Marcel Tijsterman
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Curr Biol 12:1535-40. 2002
    ..Our data indicate that at least two members of this family are required for complete and effective RNAi in C. elegans...
  46. ncbi request reprint Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi
    Femke Simmer
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Curr Biol 12:1317-9. 2002
    ..The rrf-3 strain can be useful to study genes that, in wild-type, do not show a phenotype after RNAi, and it is probably the strain of choice for genome-wide RNAi screens...
  47. pmc Frequent germline mutations and somatic repeat instability in DNA mismatch-repair-deficient Caenorhabditis elegans
    Marcel Tijsterman
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT, Utrecht, The Netherlands
    Genetics 161:651-60. 2002
    ..elegans as a model system to discover genes involved in maintaining DNA stability by large-scale RNAi screens...
  48. ncbi request reprint Ronald H.A. Plasterk. Interview by Suzanne Berry
    Ronald H A Plasterk
    Trends Biotechnol 20:224-5. 2002
  49. ncbi request reprint The microRNA-producing enzyme Dicer1 is essential for zebrafish development
    Erno Wienholds
    Department of Functional Genomics, Hubrecht Laboratory, 3584 CT Utrecht, The Netherlands
    Nat Genet 35:217-8. 2003
    ..This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development...
  50. ncbi request reprint Transposon silencing in the Caenorhabditis elegans germ line by natural RNAi
    Titia Sijen
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Nature 426:310-4. 2003
    ..RNAi mediated by this dsRNA silences transposase gene expression...
  51. ncbi request reprint RNA helicase MUT-14-dependent gene silencing triggered in C. elegans by short antisense RNAs
    Marcel Tijsterman
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands
    Science 295:694-7. 2002
    ..Our data favor the hypothesis that gene silencing is accomplished by RNA primer extension using the mRNA as template, leading to dsRNA that is subsequently degraded...
  52. ncbi request reprint RNAi protects the Caenorhabditis elegans germline against transposition
    Nadine L Vastenhouw
    The Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, Utrecht, The Netherlands
    Trends Genet 20:314-9. 2004
  53. pmc Loss of C. elegans BBS-7 and BBS-8 protein function results in cilia defects and compromised intraflagellar transport
    Oliver E Blacque
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, B C V5A 1S6, Canada
    Genes Dev 18:1630-42. 2004
    ..Our findings also suggest that some of the cardinal and secondary symptoms of BBS, such as obesity, diabetes, cardiomyopathy, and learning defects may result from cilia dysfunction...
  54. pmc Homologous gene targeting in Caenorhabditis elegans by biolistic transformation
    Eugene Berezikov
    Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Nucleic Acids Res 32:e40. 2004
    ..We describe a scaled up version of biolistic transformation that can be used as a method for homologous gene targeting in the worm...
  55. pmc What's new about RNAi? Meeting on siRNAs and miRNAs
    Rene F Ketting
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    EMBO Rep 5:762-5. 2004
  56. ncbi request reprint Dicers at RISC; the mechanism of RNAi
    Marcel Tijsterman
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT, Utrecht, The Netherlands
    Cell 117:1-3. 2004
    ..Two papers in this issue of Cell now demonstrate that Dicer is also essential for the execution phase of RNAi and explore the distinct requirements for Dicers in the siRNA and miRNA pathways...
  57. ncbi request reprint Genes required for systemic RNA interference in Caenorhabditis elegans
    Marcel Tijsterman
    Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Curr Biol 14:111-6. 2004
    ..Analysis of these mutants suggests that dsRNA uptake is a specific process involving several unique proteins...
  58. pmc CONREAL: conserved regulatory elements anchored alignment algorithm for identification of transcription factor binding sites by phylogenetic footprinting
    Eugene Berezikov
    Hubrecht Laboratory, Netherlands Institute for Developmental Biology, 3584 CT, Utrecht, The Netherlands
    Genome Res 14:170-8. 2004
    ..CONREAL is accessible via a Web interface at http://conreal.niob.knaw.nl/...
  59. pmc Gene interactions in the DNA damage-response pathway identified by genome-wide RNA-interference analysis of synthetic lethality
    Gijs van Haaften
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 101:12992-6. 2004
    ..Knockdown of these genes increased sensitivity to ionizing radiation and camptothecin and resulted in increased chromosomal nondisjunction. All genes have human orthologs that may play a role in human carcinogenesis...
  60. pmc Functional characterization of the adenylyl cyclase gene sgs-1 by analysis of a mutational spectrum in Caenorhabditis elegans
    Celine Moorman
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT, Utrecht, The Netherlands
    Genetics 161:133-42. 2002
    ..The sgs-1 reduction-of-function mutants are viable and have reduced locomotion rates, but do not show defects in pharyngeal pumping or life span...
  61. ncbi request reprint RNA interference spreading in C. elegans
    Robin C May
    Hubrecht Laboratory, CT Utrecht, The Netherlands
    Methods Enzymol 392:308-15. 2005
    ..Here we review our current understanding of systemic RNAi in C. elegans and draw comparisons with systemic RNAi pathways in other organisms...
  62. pmc Characterization of Caenorhabditis elegans homologs of the Down syndrome candidate gene DYRK1A
    William B Raich
    Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Genetics 163:571-80. 2003
    ..Our results suggest that increased gene dosage of human DYRK1A in trisomy 21 may disrupt the function of fully differentiated neurons and that this disruption is reversible...
  63. pmc Worms taste bitter: ASH neurons, QUI-1, GPA-3 and ODR-3 mediate quinine avoidance in Caenorhabditis elegans
    Massimo A Hilliard
    Institute of Genetics and Biophysics, Adriano Buzzati Traverso, CNR, Napoli, Italy
    EMBO J 23:1101-11. 2004
    ..We identified and cloned a new gene, qui-1, necessary for quinine and SDS avoidance. qui-1 codes for a novel protein with WD-40 domains and which is expressed in the avoidance sensory neurons ASH and ADL...
  64. pmc Mapping determinants of gene expression plasticity by genetical genomics in C. elegans
    Yang Li
    Groningen Bioinformatics Centre, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
    PLoS Genet 2:e222. 2006
    ..Our results suggest widespread genetic variation of differential expression responses to environmental impacts and demonstrate the potential of genetical genomics for mapping the molecular determinants of phenotypic plasticity...
  65. ncbi request reprint Generation of gene knockouts and mutant models in the laboratory rat by ENU-driven target-selected mutagenesis
    Bart M G Smits
    Hubrecht Laboratory, Utrecht, The Netherlands
    Pharmacogenet Genomics 16:159-69. 2006
    ..This study aims to provide the tools to manipulate the rat genome in a more directed way...
  66. pmc Genetic variation in the zebrafish
    Victor Guryev
    Hubrecht Laboratory, Netherlands Institute for Developmental Biology, 3584CT, Utrecht, The Netherlands
    Genome Res 16:491-7. 2006
    ..Overall, the levels and organization of genetic variation between and within commonly used zebrafish strains are markedly different from other laboratory model organisms, which may affect experimental design and interpretation...
  67. ncbi request reprint Approaches to microRNA discovery
    Eugene Berezikov
    Hubrecht Laboratory, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
    Nat Genet 38:S2-7. 2006
    ..How can new miRNAs be discovered and distinguished from other types of small RNA? Here we summarize current methods for identifying and validating miRNAs and discuss criteria used to define an miRNA...
  68. ncbi request reprint The Wnt/beta-catenin pathway regulates cardiac valve formation
    Adam F L Hurlstone
    Netherlands Institute for Developmental Biology, Hubrecht Laboratory and Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Nature 425:633-7. 2003
    ..Our findings identify a novel role for Wnt/beta-catenin signalling in determining endocardial cell fate...