Research Topics
Genomes and Genes | Eugene BerezikovSummaryAffiliation: Netherlands Institute of Ecology Country: The Netherlands Publications
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Detail Information
Publications
Mammalian mirtron genesEugene Berezikov
Hubrecht Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Mol Cell 28:328-36. 2007..This notion is supported by our observation of several clade-specific features of mammalian and invertebrate mirtrons...
CASCAD: a database of annotated candidate single nucleotide polymorphisms associated with expressed sequencesVictor Guryev
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584CT, Utrecht, The Netherlands
BMC Genomics 6:10. 2005..However, such information is very important for selecting the most promising and useful candidate polymorphisms for use in experimental setups...
Cloning and expression of new microRNAs from zebrafishWigard P Kloosterman
Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Nucleic Acids Res 34:2558-69. 2006..Most newly discovered miRNAs have low expression levels and are less conserved in other vertebrate species. Our cloning and expression analysis indicates that most abundant and conserved miRNAs in zebrafish are now known...
Diversity of microRNAs in human and chimpanzee brainEugene Berezikov
Hubrecht Laboratory-KNAW, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Nat Genet 38:1375-7. 2006..These data suggest that evolution of miRNAs is an ongoing process and that along with ancient, highly conserved miRNAs, there are a number of emerging miRNAs...
Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysisEugene Berezikov
Hubrecht Laboratory, 3584 CT Utrecht, The Netherlands
Genome Res 16:1289-98. 2006..Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted...
MicroRNA-directed siRNA biogenesis in Caenorhabditis elegansRégis L Corrêa
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Centre Utrecht, Utrecht, The Netherlands
PLoS Genet 6:e1000903. 2010....
Camels and zebrafish, viruses and cancer: a microRNA updateEugene Berezikov
Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Hum Mol Genet 14:R183-90. 2005....
CONREAL web server: identification and visualization of conserved transcription factor binding sitesEugene Berezikov
Hubrecht Laboratory, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Nucleic Acids Res 33:W447-50. 2005..Comparative analysis using different algorithms can be started by keyword without any prior sequence retrieval. The interface is available at http://conreal.niob.knaw.nl...
Differences in vertebrate microRNA expressionBrandon Ason
Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Proc Natl Acad Sci U S A 103:14385-9. 2006....
CDE-1 affects chromosome segregation through uridylation of CSR-1-bound siRNAsJosien C Van Wolfswinkel
Hubrecht Institute KNAW and University Medical Centre Utrecht, Uppsalalaan 8, Utrecht, The Netherlands
Cell 139:135-48. 2009..The conserved nature of CDE-1 suggests that similar sorting mechanisms may operate in other animals, including mammals...
Deep annotation of Drosophila melanogaster microRNAs yields insights into their processing, modification, and emergenceEugene Berezikov
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands
Genome Res 21:203-15. 2011..Altogether, this study lays a comprehensive foundation for the study of miRNA diversity and evolution in a complex animal model...
Approaches to microRNA discoveryEugene Berezikov
Hubrecht Laboratory, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
Nat Genet 38:S2-7. 2006..How can new miRNAs be discovered and distinguished from other types of small RNA? Here we summarize current methods for identifying and validating miRNAs and discuss criteria used to define an miRNA...
Single nucleotide polymorphisms associated with rat expressed sequencesVictor Guryev
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584CT, Utrecht, The Netherlands
Genome Res 14:1438-43. 2004..Two of these "in silico-identified knockouts" reside in interesting QTL regions. Data are publicly available via a Web interface (http://cascad.niob.knaw.nl), allowing simple and advanced search queries...
CONREAL: conserved regulatory elements anchored alignment algorithm for identification of transcription factor binding sites by phylogenetic footprintingEugene Berezikov
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, 3584 CT, Utrecht, The Netherlands
Genome Res 14:170-8. 2004..CONREAL is accessible via a Web interface at http://conreal.niob.knaw.nl/...
Evolution of microRNA diversity and regulation in animalsEugene Berezikov
Hubrecht Institute, KNAW, University Medical Center Utrecht, The Netherlands
Nat Rev Genet 12:846-60. 2011..Insights are also emerging into how the evolution of miRNA-containing regulatory networks has contributed to organismal complexity...
MicroRNA expression in zebrafish embryonic developmentErno Wienholds
Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
Science 309:310-1. 2005....
Controlling miRNA regulation in diseaseWillemijn M Gommans
Hubrecht Institute, Utrecht, The Netherlands
Methods Mol Biol 822:1-18. 2012..The interplay between these two processes defines the final protein output in a cell, and thus the normal or pathological cellular phenotype...
Zili is required for germ cell differentiation and meiosis in zebrafishSaskia Houwing
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences, University Medical Centre Utrecht, Utrecht, The Netherlands
EMBO J 27:2702-11. 2008....
A position-dependent organisation of retinoid response elements is conserved in the vertebrate Hox clustersGaell Mainguy
Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Trends Genet 19:476-9. 2003
Tracing the evolution of tissue identity with microRNAsKatrien De Mulder
Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, Utrecht, The Netherlands
Genome Biol 11:111. 2010..Comparison of microRNA expression identified tissues present in the last common ancestor of Bilaterians and put evolution of microRNAs in the context of tissue evolution...
Functional microRNA screening using a comprehensive lentiviral human microRNA expression libraryJos B Poell
Hubrecht Institute, Cancer Genomics Center, and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
BMC Genomics 12:546. 2011..Dissecting the complexities of miRNA function continues to prove challenging as miRNAs are predicted to have thousands of targets, and mRNAs can be targeted by dozens of miRNAs...
Homologous gene targeting in Caenorhabditis elegans by biolistic transformationEugene Berezikov
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Nucleic Acids Res 32:e40. 2004..We describe a scaled up version of biolistic transformation that can be used as a method for homologous gene targeting in the worm...
GENOTRACE: cDNA-based local GENOme assembly from TRACE archivesEugene Berezikov
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Bioinformatics 18:1396-7. 2002..AVAILABILITY: The package and examples of output files can be downloaded from http://rat.niob.knaw.nl/GENOTRACE..
Functionally distinct regulatory RNAs generated by bidirectional transcription and processing of microRNA lociDavid M Tyler
Department of Developmental Biology, Sloan Kettering Institute, New York, New York 10021, USA
Genes Dev 22:26-36. 2008..These findings demonstrate that antisense transcription and processing contributes to the functional diversification of miRNA genes...
Exploring conservation of transcription factor binding sites with CONREALEugene Berezikov
Hubrecht Laboratory
Methods Mol Biol 395:437-48. 2007..The web tool is particularly suited for the analysis of individual genes or genomic regions, although the underlying algorithm can also be used in high-throughput promoter analysis...
Phylogenetic shadowing and computational identification of human microRNA genesEugene Berezikov
Cell 120:21-4. 2005..Taken together, these results suggest the presence of significantly higher numbers of miRNAs in the human genome than previously estimated...
Genetic variation in the zebrafishVictor Guryev
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, 3584CT, Utrecht, The Netherlands
Genome Res 16:491-7. 2006..Overall, the levels and organization of genetic variation between and within commonly used zebrafish strains are markedly different from other laboratory model organisms, which may affect experimental design and interpretation...
The Evolution of SINEs and LINEs in the genus Chironomus (Diptera)Ekaterina Papusheva
Physiologisches Institut, , Goettingen, Germany
J Mol Evol 58:269-79. 2004....
Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplificationAsli N Silahtaroglu
Wilhelm Johannsen Centre for Functional Genome Research, Department of Cellular and Molecular Medicine, University of Copenhagen, Blegdamsvej 3, DK 2200 Copenhagen N, Denmark
Nat Protoc 2:2520-8. 2007....
LPIN2 is associated with type 2 diabetes, glucose metabolism, and body compositionYurii S Aulchenko
Department of Epidemiology and Biostatistics, Erasmus Medical Centre Rotterdam, Postbus 2040, 3000 CA Rotterdam, Netherlands
Diabetes 56:3020-6. 2007..To identify the type 2 diabetes gene located at chromosome 18p11...
A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in ZebrafishSaskia Houwing
Hubrecht Laboratory, Uppsalalaan 8, Utrecht, Netherlands
Cell 129:69-82. 2007..Furthermore, we show that piRNAs are Dicer independent and that their 3' end likely carries a 2'O-Methyl modification...
Mouse microRNA profiles determined with a new and sensitive cloning methodShuji Takada
Division of Functional Genomics, Jichi Medical University, 3311 1 Yakushiji, Shimotsukeshi, Tochigi 329 0498, Japan
Nucleic Acids Res 34:e115. 2006..Our data thus indicate that the total number of miRNAs in vertebrates is larger than previously appreciated and that the expression of these molecules is tightly controlled in a tissue- and developmental stage-specific manner...
