Research Topics
| David JeffriesSummaryAffiliation: Medical Research Council Laboratories Country: The Gambia Publications
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Detail Information
Publications
Analysis of flow cytometry data using an automatic processing toolDavid Jeffries
Medical Research Council MRC Gambia, Banjul, The Gambia
Cytometry A 73:857-67. 2008..By applying consistent criteria to all data generated by a study, the APT can provide a level of objectivity that is difficult to match using conventional manual analysis...
Supervised learning for the automated transcription of spacer classification from spoligotype filmsDavid J Jeffries
MRC Laboratories, Fajara, The Gambia
BMC Bioinformatics 10:248. 2009..To facilitate processing of spoligotype images we have developed tools and algorithms to automate the classification and transcription of these data directly to a database while allowing for manual editing...- Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infantsDavid J C Miles
MRC Laboratories Gambia, Banjul, The Gambia
PLoS ONE 3:e2905. 2008.... - Field testing of different chemical combinations as odour baits for trapping wild mosquitoes in The GambiaMusa Jawara
Medical Research Council Laboratories, Fajara, Banjul, The Gambia
PLoS ONE 6:e19676. 2011.... - Are plasma biomarkers of immune activation predictive of HIV progression: a longitudinal comparison and analyses in HIV-1 and HIV-2 infections?Samuel Nyamweya
Medical Research Council UK, Banjul, The Gambia, West Africa
PLoS ONE 7:e44411. 2012..Their evaluation in cohort resources is therefore needed to further their development and use in HIV research... - Immune reconstitution inflammatory syndrome and the influence of T regulatory cells: a cohort study in The GambiaIrfan Zaidi
Medical Research Council UK Unit, Fajara, The Gambia
PLoS ONE 7:e39213. 2012..The levels of T regulatory cells were measured using flow cytometry at baseline and all follow-up visits. Baseline cytokine levels of IL-2, IL-6, IFN-γ, TNF-α, MIP-1β, IL-1, IL-12, IL-13, and IL-10 were measured in all patients... - Cytomegalovirus infection in Gambian infants leads to profound CD8 T-cell differentiationDavid J C Miles
MRC Laboratories Gambia, P O Box 273, Banjul, The Gambia
J Virol 81:5766-76. 2007.... 
Innate immune responses to human malaria: heterogeneous cytokine responses to blood-stage Plasmodium falciparum correlate with parasitological and clinical outcomesMichael Walther
Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Churchill Hospital, Oxford, United Kingdom
J Immunol 177:5736-45. 2006..Furthermore, the in vitro observations on cytokine kinetics presented here, suggest that intact schizont-stage infected RBC can trigger innate responses before rupture of the infected RBC...- Incidence of tuberculosis and survival after its diagnosis in patients infected with HIV-1 and HIV-2Marianne A B van der Sande
London School of Hygiene and Tropical Medicine, London, UK
AIDS 18:1933-41. 2004..HIV-2 has been associated with less immune suppression, slower disease progression and longer survival... - Changes in viral load and HBsAg and HBeAg status with age in HBV chronic carriers in The GambiaMaimuna E Mendy
Medical Research Council Laboratories, Atlantic Boulevard, PO Box 273, Banjul, The Gambia
Virol J 5:49. 2008..The aim of this study is to determine the natural course of HBV chronic infection, particularly in relation to sequential changes in serum HBV DNA levels and hepatitis B surface (HBsAg) antigen/hepatitis e antigen (HBeAg) status by age... - Long-term protection against HBV chronic carriage of Gambian adolescents vaccinated in infancy and immune response in HBV booster trial in adolescenceMarianne A B van der Sande
Medical Research Council Laboratories, Fajara, The Gambia
PLoS ONE 2:e753. 2007..These children are now in adolescence and approaching adulthood, when the onset of sexual activity may challenge their hepatitis B immunity. Thus a booster dose in adolescence could be important to maintain long-term protection... - Comparison of two interferon gamma release assays in the diagnosis of Mycobacterium tuberculosis infection and disease in The GambiaIfedayo M O Adetifa
Bacterial Diseases Programme, Medical Research Council Laboratories, Fajara, The Gambia
BMC Infect Dis 7:122. 2007..We compared the diagnostic performance of an in-house T -cell and commercial whole blood-based IGRAs for the diagnosis of LTBI and TB disease in The Gambia... - Bioactive TGF-beta levels can be preserved in plasma samples collected into heparin but not EDTAMichael Walther
Medical Research Council Laboratories, Fajara, PO Box 273, Banjul, Gambia
Cytokine 48:267-72. 2009..Where precise measurement of latent TGF-beta in blood samples is required, samples need to be collected on ice; bioactive TGF-beta can be detected reliably in samples collected into heparin tubes even when stored at RT... - Comparison of electronic data capture (EDC) with the standard data capture method for clinical trial dataBrigitte Walther
Statistics and Data Management Department, Medical Research Council, Fajara, The Gambia
PLoS ONE 6:e25348. 2011..It was performed in a West African setting, where clinical trials involve data collection from urban, rural and often remote locations... - Conjunctival FOXP3 expression in trachoma: do regulatory T cells have a role in human ocular Chlamydia trachomatis infection?Nkoyo Faal
Medical Research Council Laboratories, Fajara, Banjul, Gambia
PLoS Med 3:e266. 2006..This is essential for the successful development of a vaccine against both ocular and genital C. trachomatis infection... - Differential effects of frozen storage on the molecular detection of bacterial taxa that inhabit the nasopharynxBrenda A Kwambana
Bacterial Diseases Programme, Medical Research Council Laboratories UK, Banjul, The Gambia
BMC Clin Pathol 11:2. 2011..abstract:.. - Distinct roles for FOXP3 and FOXP3 CD4 T cells in regulating cellular immunity to uncomplicated and severe Plasmodium falciparum malariaMichael Walther
Medical Research Council Laboratories, Fajara, Banjul, The Gambia
PLoS Pathog 5:e1000364. 2009..These cells are more prevalent in children with uncomplicated malaria than in those with severe disease, suggesting that they may be the regulators of acute malarial inflammation... - Optimizing odor-baited trap methods for collecting mosquitoes during the malaria season in The GambiaMusa Jawara
Medical Research Council Laboratories, Fajara, Banjul, The Gambia
PLoS ONE 4:e8167. 2009..To optimize the use of counter-flow traps baited with human odor (nylon socks that had been worn for a single day) to capture wild mosquitoes in the Gambia, investigations were conducted at a field experimental site... - Elevated iron status strongly predicts mortality in West African adults with HIV infectionJoann M McDermid
Medical Research Council International Nutrition Group, Nutrition and Public Health Intervention Research Unit, London School of Hygiene and Tropical Medicine, Keppel Street, London, England, WC1E 7HT
J Acquir Immune Defic Syndr 46:498-507. 2007..To comprehensively assess iron status and determine whether elevated iron status, like anemia, predicts mortality... - A simple approach to test for interaction between intervention and an individual-level variable in community randomized trialsYin Bun Cheung
MRC Tropical Epidemiology Group, London School of Hygiene and Tropical Medicine, London, UK
Trop Med Int Health 13:247-55. 2008..e. small number of communities but large number of subjects per community... - A relational database for management of flow cytometry and ELISpot clinical trial dataJames S Cavenaugh
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK
Cytometry B Clin Cytom 72:49-62. 2007..Almost no published guidance exists for immunologists to design appropriate and useful data management systems... - Conjunctival chlamydial 16S ribosomal RNA expression in trachoma: is chlamydial metabolic activity required for disease to develop?Matthew J Burton
London School of Hygiene and Tropical Medicine, London, United Kingdom
Clin Infect Dis 42:463-70. 2006..We measured chlamydial 16S ribosomal RNA (rRNA) expression, a marker of chlamydial metabolic activity, in comparison with the quantitation of a chlamydial DNA target in subjects exposed to trachoma... - Cytokine and fibrogenic gene expression in the conjunctivas of subjects from a Gambian community where trachoma is endemicMatthew J Burton
International Centre for Eye Health, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, United Kingdom
Infect Immun 72:7352-6. 2004..Markers indicative of T-cell response (gamma interferon, IL-4, IL-12p40, and perforin) were increased when chlamydial infection was present... - Increased expression of mRNA encoding interleukin (IL)-4 and its splice variant IL-4delta2 in cells from contacts of Mycobacterium tuberculosis, in the absence of in vitro stimulationHelen A Fletcher
The Centre for Infectious Diseases and International Health, Windeyer Institute of Medical Sciences, Royal Free and University College Medical School, London, UK
Immunology 112:669-73. 2004..tuberculosis induces unusual, previously unrecognized, immunological events. We tentatively hypothesize that progression to active disease might depend upon the underlying ratio of IL-4 to IL-4delta2...