J W M Heemskerk

Summary

Affiliation: Maastricht University
Country: The Netherlands

Publications

  1. ncbi request reprint The Ca2+-mobilizing potency of alpha-thrombin and thrombin-receptor-activating peptide on human platelets -- concentration and time effects of thrombin-induced Ca2+ signaling
    J W Heemskerk
    Department of Biochemistry, Maastricht University, The Netherlands
    Eur J Biochem 249:547-55. 1997
  2. pmc Functional divergence of platelet protein kinase C (PKC) isoforms in thrombus formation on collagen
    Karen Gilio
    Department of Physiology and Pharmacology, School of Medical Sciences, Bristol University, Bristol BS8 1TD, United Kingdom
    J Biol Chem 285:23410-9. 2010
  3. ncbi request reprint Multiple ways to switch platelet integrins on and off
    J M E M Cosemans
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    J Thromb Haemost 6:1253-61. 2008
  4. ncbi request reprint Platelet activation and blood coagulation
    Johan W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, The Netherlands
    Thromb Haemost 88:186-93. 2002
  5. ncbi request reprint Control of platelet activation by cyclic AMP turnover and cyclic nucleotide phosphodiesterase type-3
    Marion A H Feijge
    Department of Biochemistry, University of Maastricht, P O Box 616, Maastricht, MD 6200, The Netherlands
    Biochem Pharmacol 67:1559-67. 2004
  6. ncbi request reprint Function of glycoprotein VI and integrin alpha2beta1 in the procoagulant response of single, collagen-adherent platelets
    J W Heemskerk
    Department of Biochemistry, University of Maastricht, The Netherlands
    Thromb Haemost 81:782-92. 1999
  7. pmc Ragged spiking of free calcium in ADP-stimulated human platelets: regulation of puff-like calcium signals in vitro and ex vivo
    J W Heemskerk
    Department of Biochemistry, Maastricht University, The Netherlands
    J Physiol 535:625-35. 2001
  8. ncbi request reprint Receptors and signalling mechanisms in the procoagulant response of platelets
    J W Heemskerk
    Department of Biochemistry and Human Biology, University of Maastricht, The Netherlands
    Platelets 11:301-6. 2000
  9. doi request reprint Signaling role of CD36 in platelet activation and thrombus formation on immobilized thrombospondin or oxidized low-density lipoprotein
    R Nergiz-Unal
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    J Thromb Haemost 9:1835-46. 2011
  10. doi request reprint Collagen-mimetic peptides mediate flow-dependent thrombus formation by high- or low-affinity binding of integrin alpha2beta1 and glycoprotein VI
    I C A Munnix
    Department of Biochemistry, CARIM, University of Maastricht, Maastricht, The Netherlands
    J Thromb Haemost 6:2132-42. 2008

Collaborators

Detail Information

Publications67

  1. ncbi request reprint The Ca2+-mobilizing potency of alpha-thrombin and thrombin-receptor-activating peptide on human platelets -- concentration and time effects of thrombin-induced Ca2+ signaling
    J W Heemskerk
    Department of Biochemistry, Maastricht University, The Netherlands
    Eur J Biochem 249:547-55. 1997
    ..Thus, the high and prolonged Ca2+ signal induced by alpha-thrombin is due to continuous receptor cleavage without desensitizing effects of previously cleaved receptors...
  2. pmc Functional divergence of platelet protein kinase C (PKC) isoforms in thrombus formation on collagen
    Karen Gilio
    Department of Physiology and Pharmacology, School of Medical Sciences, Bristol University, Bristol BS8 1TD, United Kingdom
    J Biol Chem 285:23410-9. 2010
    ....
  3. ncbi request reprint Multiple ways to switch platelet integrins on and off
    J M E M Cosemans
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    J Thromb Haemost 6:1253-61. 2008
    ..Back-shifting of activated integrins towards their resting state may be a novel goal of antithrombotic medication...
  4. ncbi request reprint Platelet activation and blood coagulation
    Johan W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, The Netherlands
    Thromb Haemost 88:186-93. 2002
    ..In addition, platelets can support the initiation phase of coagulation by providing binding sites for prothrombin and factor XI. They thereby take over the initiating role of tissue factor and factor VIIa in coagulation activation...
  5. ncbi request reprint Control of platelet activation by cyclic AMP turnover and cyclic nucleotide phosphodiesterase type-3
    Marion A H Feijge
    Department of Biochemistry, University of Maastricht, P O Box 616, Maastricht, MD 6200, The Netherlands
    Biochem Pharmacol 67:1559-67. 2004
    ..This crucial role of PDE3, but not of PDE5, extends to all major processes in thrombus formation: assembly of platelets into aggregates, secretion of autocrine products, and procoagulant activity...
  6. ncbi request reprint Function of glycoprotein VI and integrin alpha2beta1 in the procoagulant response of single, collagen-adherent platelets
    J W Heemskerk
    Department of Biochemistry, University of Maastricht, The Netherlands
    Thromb Haemost 81:782-92. 1999
    ..Influx of Ca2+ is required for this response, and adhesion via integrin alpha2beta1 serves to potentiate the signaling effects of GpVI...
  7. pmc Ragged spiking of free calcium in ADP-stimulated human platelets: regulation of puff-like calcium signals in vitro and ex vivo
    J W Heemskerk
    Department of Biochemistry, Maastricht University, The Netherlands
    J Physiol 535:625-35. 2001
    ..This platelet signal appears to be similar in an environment of non-physiological buffer medium and in flowing, whole blood...
  8. ncbi request reprint Receptors and signalling mechanisms in the procoagulant response of platelets
    J W Heemskerk
    Department of Biochemistry and Human Biology, University of Maastricht, The Netherlands
    Platelets 11:301-6. 2000
    ..Thus, platelets express high coagulation-promoting activity only after interacting with multiple receptors...
  9. doi request reprint Signaling role of CD36 in platelet activation and thrombus formation on immobilized thrombospondin or oxidized low-density lipoprotein
    R Nergiz-Unal
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    J Thromb Haemost 9:1835-46. 2011
    ..Platelets abundantly express glycoprotein CD36 with thrombospondin-1 (TSP1) and oxidized low-density lipoprotein (oxLDL) as proposed ligands. How these agents promote platelet activation is still poorly understood...
  10. doi request reprint Collagen-mimetic peptides mediate flow-dependent thrombus formation by high- or low-affinity binding of integrin alpha2beta1 and glycoprotein VI
    I C A Munnix
    Department of Biochemistry, CARIM, University of Maastricht, Maastricht, The Netherlands
    J Thromb Haemost 6:2132-42. 2008
    ....
  11. doi request reprint Platelet-based coagulation: different populations, different functions
    J W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    J Thromb Haemost 11:2-16. 2013
    ..Furthermore, we discuss the ligands, platelet receptors and downstream intracellular signaling pathways implicated in these coagulant functions. These insights lead to an adapted model of platelet-based coagulation...
  12. doi request reprint Perioperative dilutional coagulopathy treated with fresh frozen plasma and fibrinogen concentrate: a prospective randomized intervention trial
    M D Lancé
    Department of Anesthesiology, CARIM, Maastricht University Medical Center, Maastricht, The Netherlands
    Vox Sang 103:25-34. 2012
    ..This was tested in a controlled clinical intervention trial with patients experiencing massive bleeding during major surgery...
  13. ncbi request reprint Additive roles of platelets and fibrinogen in whole-blood fibrin clot formation upon dilution as assessed by thromboelastometry
    M Ninivaggi
    J W M Heemskerk, Department of Biochemistry CARIM, Maastricht University, P O Box 616, 6200 MD Maastricht, The Netherlands, Tel 31 43 3881671, Fax 31 43 3884159, E mail
    Thromb Haemost 111:447-57. 2014
    ..Clinical decisions for transfusion based on thromboelastometry should take into account the platelet concentration. ..
  14. doi request reprint Impaired thrombin generation and fibrin clot formation in patients with dilutional coagulopathy during major surgery
    S E M Schols
    Department of Biochemistry CARIM, Maastricht University, P O Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 103:318-28. 2010
    ..In conclusion, the ability of thrombin generation and fibrin clot formation is independently reduced in acquired dilutional coagulopathy, while minimal levels of both are required for adequate haemostasis...
  15. ncbi request reprint von Willebrand factor stimulates thrombin-induced exposure of procoagulant phospholipids on the surface of fibrin-adherent platelets
    J J Briedé
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    J Thromb Haemost 1:559-65. 2003
    ..6 micro mol L(-1). Taken together, these results demonstrate that thrombin-induced exposure of PS and high calcium response on fibrin-adherent platelets depends on shear- or botrocetin-induced VWF-platelet interaction...
  16. doi request reprint Complementary roles of platelets and coagulation in thrombus formation on plaques acutely ruptured by targeted ultrasound treatment: a novel intravital model
    M J E Kuijpers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    J Thromb Haemost 7:152-61. 2009
    ..Atherothrombosis is a major cause of cardiovascular events. However, animal models to study this process are scarce...
  17. ncbi request reprint Classification of venous thromboembolism (VTE). Platelets in venous thrombosis
    P E J Meijden
    Department of Biochemistry, CARIM, University of Maastricht and University Hospital Maastricht, Maastricht, The Netherlands
    J Thromb Haemost 3:2575-7. 2005
  18. doi request reprint Potentiating role of Gas6 and Tyro3, Axl and Mer (TAM) receptors in human and murine platelet activation and thrombus stabilization
    J M E M Cosemans
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht, The Netherlands
    J Thromb Haemost 8:1797-808. 2010
    ..Interaction of murine Gas6 with the platelet Gas6 receptors Tyro3, Axl and Mer (TAM) plays an important role in arterial thrombus formation. However, a role for Gas6 in human platelet activation has been questioned...
  19. ncbi request reprint CD36 as a multiple-ligand signaling receptor in atherothrombosis
    R Nergiz-Unal
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, The Netherlands
    Cardiovasc Hematol Agents Med Chem 9:42-55. 2011
    ..The new insights make CD36 attractive as a therapeutic target to suppress platelet and monocyte/macrophage function and thereby atherothrombosis...
  20. doi request reprint Role of newly formed platelets in thrombus formation in rat after clopidogrel treatment: comparison to the reversible binding P2Y₁₂ antagonist ticagrelor
    Marijke J E Kuijpers
    Dept of Biochemistry, CARIM, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 106:1179-88. 2011
    ..This difference is masked by conventional platelet aggregation methods, but is revealed by thrombus formation measurement under flow. Juvenile platelets formed at later time points after clopidogrel treatment promoted thrombus formation...
  21. ncbi request reprint Dual P2Y 12 receptor signaling in thrombin-stimulated platelets--involvement of phosphoinositide 3-kinase beta but not gamma isoform in Ca2+ mobilization and procoagulant activity
    Paola E J van der Meijden
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, University of Maastricht, Maastricht, The Netherlands
    FEBS J 275:371-85. 2008
    ..Taken together, these results demonstrate a dual P2Y(12)-dependent signaling mechanism, which increases the platelet-activating effect of thrombin by prolongation of Ca(2+) elevation, thereby facilitating the coagulation process...
  22. ncbi request reprint Role of murine integrin alpha2beta1 in thrombus stabilization and embolization: contribution of thromboxane A2
    Marijke J E Kuijpers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    Thromb Haemost 98:1072-80. 2007
    ..The increased embolization in alpha2-null mice may argue against the use of alpha2beta1 integrin inhibitors for antithrombotic therapy...
  23. pmc Segregation of platelet aggregatory and procoagulant microdomains in thrombus formation: regulation by transient integrin activation
    Imke C A Munnix
    Department of Biochemistry, Maastricht University, CARIM, Maastricht University, The Netherlands
    Arterioscler Thromb Vasc Biol 27:2484-90. 2007
    ..Platelets play a dual role in thrombosis by forming aggregates and stimulating coagulation. We investigated the commitment of platelets to these separate functions during collagen-induced thrombus formation in vitro and in vivo...
  24. ncbi request reprint The quercetin paradox
    Agnes W Boots
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands
    Toxicol Appl Pharmacol 222:89-96. 2007
    ..Apparently, the potential toxicity of metabolites formed during the actual antioxidant activity of free radical scavengers should be considered in antioxidant supplementation...
  25. ncbi request reprint Both ADP and thrombin regulate arteriolar thrombus stabilization and embolization, but are not involved in initial hemostasis as induced by micropuncture
    Miriam A van Gestel
    Department of Physiology, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    Microcirculation 14:193-205. 2007
    ..Up to now, the relative importance of mediators involved is only partly known. It was the aim of this study to investigate the involvement of ADP and thrombin in subsequent phases of arteriolar hemostasis and thromboembolism in vivo...
  26. ncbi request reprint Dual role of platelet protein kinase C in thrombus formation: stimulation of pro-aggregatory and suppression of procoagulant activity in platelets
    Amrei Strehl
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, 6200 MD Maastricht, The Netherlands
    J Biol Chem 282:7046-55. 2007
    ..This platelet signaling protein is the first one identified to balance the pro-aggregatory and procoagulant functions of thrombi...
  27. ncbi request reprint Plasma triacylglycerol and coagulation factor concentrations predict the anticoagulant effect of dietary fish oil in overweight subjects
    Kristof Vanschoonbeek
    Department of Human Biology, Nutrition and Toxicology Research and Cardiovascular Research Institutes of Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
    J Nutr 137:7-13. 2007
    ..We conclude that high fasting triacylglycerol concentrations predict high procoagulant activity and a lowering of thrombin potential with dietary fish oil...
  28. ncbi request reprint Novel methodology for assessment of prophylactic platelet transfusion therapy by measuring increased thrombus formation and thrombin generation
    Sandra Cauwenberghs
    Department of Biochemistry CARIM, Maastricht University, Maastricht, The Netherlands
    Br J Haematol 136:480-90. 2007
    ..We conclude that assessment of flow-induced adhesion and thrombin generation in acquired thrombocytopenia adequately determines the improved haemostatic activity by transfused platelets...
  29. ncbi request reprint Real-time detection of activation patterns in individual platelets during thromboembolism in vivo: differences between thrombus growth and embolus formation
    Miriam A van Gestel
    Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, NL 6200 MD Maastricht, The Netherlands
    J Vasc Res 39:534-43. 2002
    ..These results show, for the first time, the relation between single platelet activation patterns, which are different during thrombus growth and embolus formation, and their behavior in a thromboembolic process in vivo...
  30. doi request reprint Key role of platelet procoagulant activity in tissue factor-and collagen-dependent thrombus formation in arterioles and venules in vivo differential sensitivity to thrombin inhibition
    Marijke J E Kuijpers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    Microcirculation 15:269-82. 2008
    ..We investigated the interplay of these processes in vivo in a mouse model of arteriolar and venular thrombus formation...
  31. ncbi request reprint Thrombin-induced hyperactivity of platelets of young stroke patients: involvement of thrombin receptors in the subject-dependent variability in Ca2+ signal generation
    Kristof Vanschoonbeek
    Dept of Human Biology, Cardiovascular Research Institute of Maastricht, University of Maastricht, Maastricht, The Netherlands
    Thromb Haemost 88:931-7. 2002
    ..Furthermore, the subject-dependent variation in thrombin-induced signalling is likely to involve PAR1-mediated platelet activation...
  32. doi request reprint Anticoagulant effect of dietary fish oil in hyperlipidemia: a study of hepatic gene expression in APOE2 knock-in mice
    Kristof Vanschoonbeek
    Department of Biochemistry, CARIM and NUTRIM, Maastricht University, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 28:2023-9. 2008
    ..Using APOE2 knock-in mice, the relation of these fish-oil effects with altered gene expression was investigated...
  33. ncbi request reprint Overexpression of the platelet P2X1 ion channel in transgenic mice generates a novel prothrombotic phenotype
    Cecile Oury
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Blood 101:3969-76. 2003
    ..Activation of the ERK2 pathway is instrumental in these processes...
  34. ncbi request reprint Activation of alphaIIbbeta3 is a sufficient but also an imperative prerequisite for activation of alpha2beta1 on platelets
    Gerlinde R Van De Walle
    Laboratory for Thrombosis Research, Katholieke Universiteit Leuven, KU Leuven Campus Kortrijk, E Sabbelaan 53, B 8500 Kortrijk, Belgium, and Institut Fédératif No 4, Hopital Cardiologique, Pessac, France
    Blood 109:595-602. 2007
    ..In addition, analogous experiments with Glanzmann platelets lacking alphaIIbbeta3 confirmed the indispensability of alphaIIbbeta3 for alpha2beta1 activation...
  35. doi request reprint Role of membrane cholesterol in platelet calcium signalling in response to VWF and collagen under stasis and flow
    Marjolijn van Lier
    Department of Clinical Chemistry and Hematology and Cell Microscopy Center, UMCU, Utrecht, The Netherlands
    Thromb Haemost 99:1068-78. 2008
    ..Our data thus reveal a more critical role for membrane cholesterol in collagen-induced than in VWF-induced Ca(2+) signalling, and furthermore support the concept that secondary activation responses are dependent on intact CRDs...
  36. ncbi request reprint Development of platelet inhibition by cAMP during megakaryocytopoiesis
    Els den Dekker
    Laboratory for Thrombosis and Haemostasis, Department of Haematology, University Medical Center Utrecht, Utrecht University, 3508 GA Utrecht, The Netherlands
    J Biol Chem 277:29321-9. 2002
    ..Thus, up-regulation of PKA-C appears to be a key step in the development of Ca2+ inhibition by prostacyclin in platelets...
  37. ncbi request reprint Irregular spiking in free calcium concentration in single, human platelets. Regulation by modulation of the inositol trisphosphate receptors
    Roosje M A van Gorp
    Department of Biochemistry, University of Maastricht, The Netherlands
    Eur J Biochem 269:1543-52. 2002
    ..The observation that individual Ca2+ peaks were generated in various parts of a platelet at similar intervals and amplitudes points to effective cooperation of the various stores in the Ca2+-release process...
  38. ncbi request reprint Cyclic AMP raises intracellular Ca(2+) in human megakaryocytes independent of protein kinase A
    Els den Dekker
    Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center Utrecht, The Netherlands
    Arterioscler Thromb Vasc Biol 22:179-86. 2002
    ..These findings reveal a novel mechanism for elevating cytosolic Ca(2+) by cAMP, possibly via GTP-Rap1...
  39. ncbi request reprint Shedding of procoagulant microparticles from unstimulated platelets by integrin-mediated destabilization of actin cytoskeleton
    Sandra Cauwenberghs
    Department of Biochemistry, CARIM, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
    FEBS Lett 580:5313-20. 2006
    ..After transfusion of stored platelet preparations to thrombocytopenic patients, the microparticles contribute to coagulant activity in vivo...
  40. ncbi request reprint In vivo blockade of platelet ADP receptor P2Y12 reduces embolus and thrombus formation but not thrombus stability
    Miriam A van Gestel
    Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 23:518-23. 2003
    ..ADP is a key platelet agonist in thromboembolism. One of the receptors involved in ADP-induced platelet activation is the P2Y12 receptor, which is a target for antithrombotic drugs...
  41. ncbi request reprint Adhesion of human and mouse platelets to collagen under shear: a unifying model
    Jocelyn M Auger
    Centre for Cardiovascular Sciences, Division of Medical Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Edgbaston, Birmingham, UK
    FASEB J 19:825-7. 2005
    ..These observations form the basis of a unifying two-state model of platelet adhesion and aggregate formation on collagen that is conserved between human and mouse platelets...
  42. ncbi request reprint Regulation of tissue factor-induced coagulation and platelet aggregation in flowing whole blood
    Marijke J E Kuijpers
    Department of Biochemistry, CARIM, University of Maastricht, P O Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 93:97-105. 2005
    ..These platelet inhibitors thus maintain their aggregation-inhibiting effect at sites of thrombin formation...
  43. ncbi request reprint Principal role of glycoprotein VI in alpha2beta1 and alphaIIbbeta3 activation during collagen-induced thrombus formation
    Christelle Lecut
    Department of Biochemistry, CARIM, Maastricht University, The Netherlands
    Arterioscler Thromb Vasc Biol 24:1727-33. 2004
    ..High-shear perfusion of blood over collagen results in rapid platelet adhesion, aggregation, and procoagulant activity. We studied regulation of alpha2beta1 and alphaIIbbeta3 integrin activation during thrombus formation on collagen...
  44. ncbi request reprint Variable hypocoagulant effect of fish oil intake in humans: modulation of fibrinogen level and thrombin generation
    Kristof Vanschoonbeek
    Department of Human Biology, Nutrition and Toxicology Research and Cardiovascular Research Institutes of Maastricht, Maastricht University, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 24:1734-40. 2004
    ..We studied how fish oil intake influenced thrombin generation in plasma and which factors were involved herein...
  45. pmc Facilitating roles of murine platelet glycoprotein Ib and alphaIIbbeta3 in phosphatidylserine exposure during vWF-collagen-induced thrombus formation
    Marijke J E Kuijpers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    J Physiol 558:403-15. 2004
    ..Hence, these glycoproteins have an assistant procoagulant role in collagen-dependent thrombus formation, which is most prominent at reduced GPVI activity and is independent of the presence of thrombin...
  46. ncbi request reprint Fish oil consumption and reduction of arterial disease
    Kristof Vanschoonbeek
    Department of Human Biology, University of Maastricht, The Netherlands
    J Nutr 133:657-60. 2003
    ..In addition, we consider the possibility that the dietary effect on hemostatic and lipid factors involves transcription regulation of multiple genes, perhaps in a subject-dependent manner...
  47. ncbi request reprint Decreased responsiveness and development of activation markers of PLTs stored in plasma
    Joyce Curvers
    Sanquin Blood Bank Southeast Region, Maastricht, The Netherlands
    Transfusion 44:49-58. 2004
    ..PLTs collected for transfusion purposes preferably have retained these properties to restore impaired hemostasis with thrombocytopenia...
  48. ncbi request reprint Platelet receptor interplay regulates collagen-induced thrombus formation in flowing human blood
    Pia R M Siljander
    Department of Biochemistry, University of Cambridge, Main Bldg, Downing Site, Tennis Court Rd, CB2 1QW Cambridge, United Kingdom
    Blood 103:1333-41. 2004
    ....
  49. ncbi request reprint Integrin alphaIIbbeta3 and shear-dependent action of glycoprotein Ibalpha stimulate platelet-dependent thrombin formation in stirred plasma
    Jeffrey F W Keuren
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University Maastricht, Maastricht, The Netherlands
    J Lab Clin Med 141:350-8. 2003
    ..We conclude that thrombin formation and coagulation in stirred PRP depend, to a large extent, on platelet adhesion to integrin alpha(IIb)beta(3) and, in a shear-dependent way, on GP Ib...
  50. ncbi request reprint Store-mediated calcium entry in the regulation of phosphatidylserine exposure in blood cells from Scott patients
    Imke C A Munnix
    Department of Biochemistry, CARIM, Maastricht University, P O Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 89:687-95. 2003
    ..patient V.W. is a new case of Scott syndrome, ii. Ca(2+) entry in the platelets and lymphoblasts from both Scott patients is normal, and iii. elevated [Ca(2+)](i) as caused by SMCE is not sufficient to trigger PS exposure...
  51. ncbi request reprint Synergistic effect of thrombin on collagen-induced platelet procoagulant activity is mediated through protease-activated receptor-1
    Jeffrey F W Keuren
    Sanquin, Blood Bank region South East, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 25:1499-505. 2005
    ..The objective of this study is to elucidate the mechanism of this synergistic action with a focus on the intracellular Ca2+ concentration ([Ca2+]i) and the various platelet receptors for thrombin...
  52. ncbi request reprint The glycoprotein VI-phospholipase Cgamma2 signaling pathway controls thrombus formation induced by collagen and tissue factor in vitro and in vivo
    Imke C A Munnix
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 25:2673-8. 2005
    ..We investigated the signaling pathway of collagen-induced platelet activation in interaction with tissue factor-triggered coagulation during the thrombus-forming process...
  53. ncbi request reprint Platelet inhibition by insulin is absent in type 2 diabetes mellitus
    Irlando Andrade Ferreira
    Department of Hematology, University Medical Center Utrecht, Utrecht University, The Netherlands
    Arterioscler Thromb Vasc Biol 26:417-22. 2006
    ..We demonstrated recently in platelets from healthy subjects that insulin interferes with Ca2+ increases induced by ADP-P2y1 contact through blockade of the G-protein Gi, and thereby with P2y12-mediated suppression of cAMP...
  54. ncbi request reprint Complementary roles of glycoprotein VI and alpha2beta1 integrin in collagen-induced thrombus formation in flowing whole blood ex vivo
    Marijke J E Kuijpers
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    FASEB J 17:685-7. 2003
    ....
  55. ncbi request reprint Platelet ADP response deteriorates in synthetic storage media
    Jeffrey F W Keuren
    Sanquin Blood Bank Southeast Region, Maastricht, The Netherlands
    Transfusion 46:204-12. 2006
    ..The goal of this study was to evaluate the ADP-induced responses of PLTs stored in full plasma or in plasma and additive solution (AS)...
  56. ncbi request reprint Fibrillar type I collagens enhance platelet-dependent thrombin generation via glycoprotein VI with direct support of alpha2beta1 but not alphaIIbbeta3 integrin
    Christelle Lecut
    Department Biochemistry, CARIM, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 94:107-14. 2005
    ....
  57. ncbi request reprint Plasma ectonucleotidases prevent desensitization of purinergic receptors in stored platelets: importance for platelet activity during thrombus formation
    Sandra Cauwenberghs
    Department of Biochemistry CARIM, the Sanquin Blood Bank South East, the Department of Health Risk Analysis and Toxicology NUTRIM, Maastricht University, Maastricht, The Netherlands
    Transfusion 46:1018-28. 2006
    ..In vivo, this is antagonized by ectonucleotidases on the surface of endothelial cells and white blood cells (WBCs). The receptor desensitization of ATP- and ADP-induced responses of PLTs stored in plasma without WBCs was investigated...
  58. ncbi request reprint Platelet P2Y12 receptors enhance signalling towards procoagulant activity and thrombin generation. A study with healthy subjects and patients at thrombotic risk
    Paola E J van der Meijden
    Department of Biochemistry, University of Maastricht, P O Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 93:1128-36. 2005
    ..It is similarly active in platelets from control subjects and patients at thrombotic risk. Finally, the thrombogram method is useful for measuring incomplete P2Y(12) inhibition with clopidogrel...
  59. ncbi request reprint Antithrombin extends its job
    Johan W M Heemskerk
    Thromb Haemost 92:1171. 2004
  60. ncbi request reprint Factor Xa and thrombin evoke additive calcium and proinflammatory responses in endothelial cells subjected to coagulation
    Valery Daubie
    Deparment of Biochemistry, CARIM, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
    Biochim Biophys Acta 1763:860-9. 2006
    ..These data provide first evidence that these serine proteases trigger distinct signaling modules in endothelium that is activated by plasma coagulation...
  61. doi request reprint Increased thrombin generation and fibrinogen level after therapeutic plasma transfusion: relation to bleeding
    Saskia E M Schols
    Department of Biochemistry CARIM, Maastricht University, Maastricht, The Netherlands
    Thromb Haemost 99:64-70. 2008
    ..Furthermore, the data suggest that thrombin generation and fibrinogen are independent determinants of the risk of perioperative bleeding in this patient group...
  62. ncbi request reprint Glutathione oxidation in calcium- and p38 MAPK-dependent membrane blebbing of endothelial cells
    Roosje M A van Gorp
    Department of Human Biology, Maastricht University, 6200 MD Maastricht, The Netherlands
    Biochim Biophys Acta 1591:129-138. 2002
    ....
  63. ncbi request reprint Measurement of the platelet procoagulant response
    Johan W M Heemskerk
    Department of Biochemistry, CARIM, Maastricht University, The Netherlands
    Methods Mol Biol 272:135-44. 2004
  64. ncbi request reprint Hemostatic and signaling functions of transfused platelets
    Sandra Cauwenberghs
    Department of Biochemistry CARIM, Maastricht University and University Hospital, The Netherlands
    Transfus Med Rev 21:287-94. 2007
    ....
  65. ncbi request reprint Platelet collagen receptors and coagulation. A characteristic platelet response as possible target for antithrombotic treatment
    Johan W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, University of Maastricht, The Netherlands
    Trends Cardiovasc Med 15:86-92. 2005
    ..The main signaling receptor for collagen, glycoprotein VI, plays a key role in the platelet procoagulant response during thrombus formation; this is a reason why new anti-glycoprotein-VI antibodies are promising antithrombotic tools...
  66. ncbi request reprint Contribution of platelet glycoprotein VI to the thrombogenic effect of collagens in fibrous atherosclerotic lesions
    Judith M E M Cosemans
    Department of Human Biology, CARIM, University of Maastricht, The Netherlands
    Atherosclerosis 181:19-27. 2005
    ..Together, these results indicate that in advanced plaques collagen type I is a major trigger of thrombus formation and PS exposure, acting via GPVI and ADP release, while tissue factor directly enhances coagulation...
  67. ncbi request reprint Continuous signaling via PI3K isoforms beta and gamma is required for platelet ADP receptor function in dynamic thrombus stabilization
    Judith M E M Cosemans
    Department of Biochemistry and Human Biology, Cardiovascular Research Institute Maastricht CARIM, University of Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands
    Blood 108:3045-52. 2006
    ..This novel concept of intrinsic, dynamic thrombus instability gives possibilities for the use of antiplatelet therapy...