J L Broers

Summary

Affiliation: Maastricht University
Country: The Netherlands

Publications

  1. ncbi request reprint Both lamin A and lamin C mutations cause lamina instability as well as loss of internal nuclear lamin organization
    Jos L V Broers
    Department of Molecular Cell Biology, Box 17, Cardiovascular Research Institute Maastricht CARIM, University of Maastricht, PO Box 616, NL 6200 MD Maastricht, The Netherlands
    Exp Cell Res 304:582-92. 2005
  2. ncbi request reprint Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis
    Jos L V Broers
    Department of Molecular Cell Biology, Research Institute Growth and Development GROW, University of Maastricht, Maastricht, The Netherlands
    Eur J Cell Biol 81:677-91. 2002
  3. ncbi request reprint Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins
    J L Broers
    Department of Molecular Cell Biology and Genetics, University of Maastricht, PO Box 616, The Netherlands
    J Cell Sci 112:3463-75. 1999
  4. ncbi request reprint Decreased mechanical stiffness in LMNA-/- cells is caused by defective nucleo-cytoskeletal integrity: implications for the development of laminopathies
    Jos L V Broers
    Department of Moecular Cell Biology, Cardiovascular Research Institute Maastricht, University Maastricht, PO Box 616, NL 6200 MD Maastricht, The Netherlands
    Hum Mol Genet 13:2567-80. 2004
  5. ncbi request reprint An alternative splicing product of the lamin A/C gene lacks exon 10
    B M Machiels
    Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands
    J Biol Chem 271:9249-53. 1996
  6. ncbi request reprint Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A
    N H Senden
    Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, Netherlands
    Eur J Cell Biol 69:197-213. 1996
  7. ncbi request reprint Abnormal A-type lamin organization in a human lung carcinoma cell line
    B M Machiels
    Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands
    Eur J Cell Biol 67:328-35. 1995
  8. ncbi request reprint A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers
    N H Senden
    Department of Molecular Cell Biology and Genetics, University Maastricht, The Netherlands
    J Pathol 182:13-21. 1997
  9. ncbi request reprint Neuronal differentiation is accompanied by NSP-C expression
    J Hens
    Department of Molecular Cell Biology and Genetics, Universiteit Maastricht, P O Box 616, 6200 MD Maastricht, The Netherlands
    Cell Tissue Res 292:229-37. 1998
  10. ncbi request reprint Changes in immunocytochemical detectability of proteasome epitopes depending on cell growth and fixation conditions of lung cancer cell lines
    B M Machiels
    Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands
    Eur J Cell Biol 66:282-92. 1995

Collaborators

  • Frans C S Ramaekers
  • C J Hutchison
  • N H Senden
  • G Hornstra
  • Paola Casarosa
  • Frank Baaijens
  • C V Bouten
  • Howard Worman
  • B Schutte
  • E A G Peeters
  • Ewa Walczak
  • Ewa Markiewicz
  • Normand Marceau
  • Vanja Pekovic
  • Valerie L R M Verstraeten
  • B M Machiels
  • Marc A M J van Zandvoort
  • Yvonne K Gruijthuijsen
  • R M van Gorp
  • Roland Foisner
  • Baziel van Engelen
  • Thomas von Zglinicki
  • Anne Loranger
  • Martin Lammens
  • Jens Harborth
  • Jasmin Mathew
  • Stéphane Gilbert
  • Mieke E R Henfling
  • Helma J H Kuijpers
  • Raoul C M Hennekam
  • Miriam Kamps
  • Margareth Dorobek
  • Peter M Steijlen
  • Robert G Wilson
  • Mark van de Wetering
  • Nick Barker
  • Georgia Salpingidou
  • Juliet A Ellis
  • J Hens
  • W Matthijs Blankesteijn
  • Arthur van den Wijngaard
  • Hubert J M Smeets
  • Katarzyna Tilgner
  • Sophie Zinn-Justin
  • Irena Hausmanowa-Petrusewicz
  • Diane Merckx
  • Maurice A M van Steensel
  • Hans Clevers
  • Carlo L M Marcelis
  • Cees J de Grauw
  • Martine J Smit
  • Hans C Gerritsen
  • Cathrien A Bruggeman
  • Suzanne J F Kaptein
  • Rob Leurs
  • Dick W Slaaf
  • Cornelis Vink
  • Mirjam G A oude Egbrink
  • H J Kuijpers
  • C P Reutelingsperger
  • M C van Dam-Mieras
  • N M Bronnenberg
  • J W Heemskerk
  • H Geerts
  • W J Van de Ven
  • R Nuydens
  • A J Roebroek
  • H J van de Velde
  • J M Endert
  • A H Zorenc
  • G J van Eys
  • N E Caberg
  • L De Ley
  • Y Raymond
  • M E Henfling
  • K B Hendil

Detail Information

Publications20

  1. ncbi request reprint Both lamin A and lamin C mutations cause lamina instability as well as loss of internal nuclear lamin organization
    Jos L V Broers
    Department of Molecular Cell Biology, Box 17, Cardiovascular Research Institute Maastricht CARIM, University of Maastricht, PO Box 616, NL 6200 MD Maastricht, The Netherlands
    Exp Cell Res 304:582-92. 2005
    ..Our findings suggest a loss of function of A-type lamin mutant proteins in the organization of intranuclear chromatin and predict the loss of gene regulatory function in laminopathies...
  2. ncbi request reprint Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis
    Jos L V Broers
    Department of Molecular Cell Biology, Research Institute Growth and Development GROW, University of Maastricht, Maastricht, The Netherlands
    Eur J Cell Biol 81:677-91. 2002
    ....
  3. ncbi request reprint Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins
    J L Broers
    Department of Molecular Cell Biology and Genetics, University of Maastricht, PO Box 616, The Netherlands
    J Cell Sci 112:3463-75. 1999
    ..Time-lapse studies of mitotic cells showed that reformation of the lamina and the tubular structures consisting of A-type lamins did not occur until after cytokinesis was completed...
  4. ncbi request reprint Decreased mechanical stiffness in LMNA-/- cells is caused by defective nucleo-cytoskeletal integrity: implications for the development of laminopathies
    Jos L V Broers
    Department of Moecular Cell Biology, Cardiovascular Research Institute Maastricht, University Maastricht, PO Box 616, NL 6200 MD Maastricht, The Netherlands
    Hum Mol Genet 13:2567-80. 2004
    ..e. lamins) and the cytoskeleton is causing more general cellular weakness and emphasizes a potential key function for lamins in maintaining cellular tensegrity...
  5. ncbi request reprint An alternative splicing product of the lamin A/C gene lacks exon 10
    B M Machiels
    Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands
    J Biol Chem 271:9249-53. 1996
    ..mRNA analysis in a variety of cell lines, normal colon tissue as well as carcinomas demonstrated the presence of lamin Adelta 10 in all samples examined, suggesting its presence in a variety of cell types...
  6. ncbi request reprint Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A
    N H Senden
    Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, Netherlands
    Eur J Cell Biol 69:197-213. 1996
    ..Immunoprecipitation analyses under native conditions indicate that NSP-C does not need to associate with NSP-A to form high molecular weight NSP-reticulons...
  7. ncbi request reprint Abnormal A-type lamin organization in a human lung carcinoma cell line
    B M Machiels
    Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands
    Eur J Cell Biol 67:328-35. 1995
    ..This truncated product is partly Triton X-100 soluble since it was also detected in the detergent soluble fraction.(ABSTRACT TRUNCATED AT 250 WORDS)..
  8. ncbi request reprint A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers
    N H Senden
    Department of Molecular Cell Biology and Genetics, University Maastricht, The Netherlands
    J Pathol 182:13-21. 1997
    ..It is concluded that NSP-reticulons are valuable markers in the diagnosis of neuroendocrine differentiation in non-SCLC and should be used in conjunction with NCAM...
  9. ncbi request reprint Neuronal differentiation is accompanied by NSP-C expression
    J Hens
    Department of Molecular Cell Biology and Genetics, Universiteit Maastricht, P O Box 616, 6200 MD Maastricht, The Netherlands
    Cell Tissue Res 292:229-37. 1998
    ..It is concluded that NSP-C expression, in particular, is strongly correlated with neuronal differentiation...
  10. ncbi request reprint Changes in immunocytochemical detectability of proteasome epitopes depending on cell growth and fixation conditions of lung cancer cell lines
    B M Machiels
    Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands
    Eur J Cell Biol 66:282-92. 1995
    ..The immunoblotting experiments show no quantitative changes in proteasome content of the tumor cells at the different growth conditions...
  11. ncbi request reprint A- and B-type lamins are differentially expressed in normal human tissues
    J L Broers
    Department of Molecular Cell Biology and Genetics, Universiteit Maastricht, The Netherlands
    Histochem Cell Biol 107:505-17. 1997
    ..Furthermore, most cells of neuroendocrine origin as well as most hematopoietic cells were negative for A-type lamin antibodies...
  12. ncbi request reprint Peroxide-induced membrane blebbing in endothelial cells associated with glutathione oxidation but not apoptosis
    R M van Gorp
    Department of Human Biology, Maastricht University, 6200 MD Maastricht, The Netherlands
    Am J Physiol 277:C20-8. 1999
    ..We conclude that, in endothelial cells, peroxide-induced cytoskeletal reorganization and bleb formation correlate with the degree of GSH oxidation but do not represent an early stage of the apoptotic process...
  13. ncbi request reprint Discrimination of DNA and RNA in cells by a vital fluorescent probe: lifetime imaging of SYTO13 in healthy and apoptotic cells
    Marc A M J van Zandvoort
    Department of Biophysics, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    Cytometry 47:226-35. 2002
    ..We show that fluorescence lifetime imaging using two-photon excitation of SYTO13 allows differential and simultaneous imaging of DNA and RNA in living cells, as well as sequential and repetitive assessment of staining patterns...
  14. ncbi request reprint Laminopathies
    Jos L V Broers
    Department of Molecular Cell Biology, Research Institutes CARIM, GROW, and EURON, University of Maastricht, The Netherlands
    J Pathol 204:478-88. 2004
    ..Which of these identities underlies the laminopathies, a myriad of genetic diseases caused by mutations in lamins or lamin-associated proteins, is a topic of intense debate...
  15. ncbi request reprint Cluster-10 lung-cancer antibodies recognize NSPs, novel neuro-endocrine proteins associated with membranes of the endoplasmic reticulum
    N H Senden
    Department of Molecular Cell Biology and Genetics, University of Limburg, Maastricht, The Netherlands
    Int J Cancer Suppl 8:84-8. 1994
    ..Gel filtration studies with this solubilized fraction revealed that NSPs form supramolecular aggregates with a molecular weight of more then 500 kDa...
  16. pmc The rat cytomegalovirus R33-encoded G protein-coupled receptor signals in a constitutive fashion
    Yvonne K Gruijthuijsen
    Department of Medical Microbiology, Cardiovascular Research Institute Maastricht, University of Maastricht, 6202 AZ Maastricht, The Netherlands
    J Virol 76:1328-38. 2002
    ..In contrast to its homolog of human herpesvirus 6B (pU12), pR33 does not bind RANTES...
  17. ncbi request reprint Compound heterozygosity for mutations in LMNA causes a progeria syndrome without prelamin A accumulation
    Valerie L R M Verstraeten
    Department of Dermatology, University Hospital Maastricht, P Debyelaan 25, 6202 AZ Maastricht, The Netherlands
    Hum Mol Genet 15:2509-22. 2006
    ..In contrast to earlier suggestions, we show that prelamin A accumulation is not the major determinant of the progeroid phenotype...
  18. pmc The inner nuclear membrane protein emerin regulates beta-catenin activity by restricting its accumulation in the nucleus
    Ewa Markiewicz
    Department of Biological Science, The School of Biological and Biomedical Sciences, The University of Durham, Durham, UK
    EMBO J 25:3275-85. 2006
    ..Our results support recent findings that suggest that INM proteins can influence signalling pathways by restricting access of transcription coactivators to the nucleus...
  19. pmc Nucleoplasmic LAP2alpha-lamin A complexes are required to maintain a proliferative state in human fibroblasts
    Vanja Pekovic
    School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, England, UK
    J Cell Biol 176:163-72. 2007
    ..Our results suggest that LAP2alpha and lamin A/C are involved in controlling Rb localization and phosphorylation, and a lack or mislocalization of either protein leads to cell cycle arrest in HDFs...
  20. ncbi request reprint Dual roles of intermediate filaments in apoptosis
    Normand Marceau
    Centre de recherche en cancérologie de l Université Laval and L Hôtel Dieu de Québec CHUQ, Quebec, Canada G1R 2J6
    Exp Cell Res 313:2265-81. 2007
    ..Clearly, the data accumulated in recent years provide new and significant insights into the multiple functions of IFs, particularly their dual roles in cell response to apoptotic insults...