Roel Willemze

Summary

Affiliation: Leiden University Medical Center
Country: The Netherlands

Publications

  1. ncbi request reprint Clofarabine in combination with a standard remission induction regimen (cytosine arabinoside and idarubicin) in patients with previously untreated intermediate and bad-risk acute myelogenous leukemia (AML) or high-risk myelodysplastic syndrome (HR-MDS): p
    R Willemze
    Department of Hematology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands
    Ann Hematol 93:965-75. 2014
  2. doi request reprint KIR-ligand incompatibility in the graft-versus-host direction improves outcomes after umbilical cord blood transplantation for acute leukemia
    R Willemze
    Clinical Research Unit, Eurocord Office, APHP, Hopital Saint Louis, Université de Paris VII, Paris, France
    Leukemia 23:492-500. 2009
  3. ncbi request reprint Post-remission treatment for adult patients with acute lymphoblastic leukemia in first remission: is there a role for autologous stem cell transplantation?
    Roel Willemze
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands, and University Hospital Center Rebro, Zagreb, Croatia
    Semin Hematol 44:267-73. 2007
  4. doi request reprint Is there an impact of killer cell immunoglobulin-like receptors and KIR-ligand incompatibilities on outcomes after unrelated cord blood stem cell transplantation?
    Roel Willemze
    Department of Hematology, Leiden University Medical Center, The Netherlands
    Best Pract Res Clin Haematol 23:283-90. 2010
  5. ncbi request reprint Multiple myeloma-reactive T cells recognize an activation-induced minor histocompatibility antigen encoded by the ATP-dependent interferon-responsive (ADIR) gene
    Cornelis A M van Bergen
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Blood 109:4089-96. 2007
  6. ncbi request reprint Efficiency of T-cell receptor expression in dual-specific T cells is controlled by the intrinsic qualities of the TCR chains within the TCR-CD3 complex
    Mirjam H M Heemskerk
    Department of Hematology, Leiden University Medical Center, C2 R, PO Box 9600, 2300 RC Leiden, The Netherlands
    Blood 109:235-43. 2007
  7. ncbi request reprint Functional analysis of killer Ig-like receptor-expressing cytomegalovirus-specific CD8+ T cells
    Lars T van der Veken
    Department of Hematology, Laboratory of Experimental Hematology, Leiden University Medical Center, Leiden, The Netherlands
    J Immunol 182:92-101. 2009
  8. ncbi request reprint Early detection and rapid isolation of leukemia-reactive donor T cells for adoptive transfer using the IFN-gamma secretion assay
    Inge Jedema
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Clin Cancer Res 13:636-43. 2007
  9. doi request reprint Identification of 4 new HLA-DR-restricted minor histocompatibility antigens as hematopoietic targets in antitumor immunity
    Anita N Stumpf
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Blood 114:3684-92. 2009
  10. pmc Donor-derived mesenchymal stem cells are immunogenic in an allogeneic host and stimulate donor graft rejection in a nonmyeloablative setting
    Alma J Nauta
    Laboratory of Experimental Hematology, Department of Hematology, D2 P, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
    Blood 108:2114-20. 2006

Detail Information

Publications73

  1. ncbi request reprint Clofarabine in combination with a standard remission induction regimen (cytosine arabinoside and idarubicin) in patients with previously untreated intermediate and bad-risk acute myelogenous leukemia (AML) or high-risk myelodysplastic syndrome (HR-MDS): p
    R Willemze
    Department of Hematology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands
    Ann Hematol 93:965-75. 2014
    ..Clofarabine, 5 × 10 mg/m(2)/day, resulted in one DLT and no early treatment withdrawals. MTD of clofarabine combined with cytosine arabinoside and idarubicin is 5 × 10 mg/m(2)/day. ..
  2. doi request reprint KIR-ligand incompatibility in the graft-versus-host direction improves outcomes after umbilical cord blood transplantation for acute leukemia
    R Willemze
    Clinical Research Unit, Eurocord Office, APHP, Hopital Saint Louis, Université de Paris VII, Paris, France
    Leukemia 23:492-500. 2009
    ..012), and 5 versus 36% (P=0.005), respectively). UCBT for acute leukemia in CR from KIR-ligand-incompatible donors is associated with decreased RI and improved LFS and OS...
  3. ncbi request reprint Post-remission treatment for adult patients with acute lymphoblastic leukemia in first remission: is there a role for autologous stem cell transplantation?
    Roel Willemze
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands, and University Hospital Center Rebro, Zagreb, Croatia
    Semin Hematol 44:267-73. 2007
    ..This finding may eventually have an impact on the overall survival rate. Currently, the main benefit of autoSCT may be its short duration compared with the continuation chemotherapy regimen...
  4. doi request reprint Is there an impact of killer cell immunoglobulin-like receptors and KIR-ligand incompatibilities on outcomes after unrelated cord blood stem cell transplantation?
    Roel Willemze
    Department of Hematology, Leiden University Medical Center, The Netherlands
    Best Pract Res Clin Haematol 23:283-90. 2010
    ..Therefore, the role of natural killer (NK) cell alloreactivity in UCBT is far from clear. It is too early to use a donor-recipient KIR(-ligand) algorithm for selection of a cord blood donor...
  5. ncbi request reprint Multiple myeloma-reactive T cells recognize an activation-induced minor histocompatibility antigen encoded by the ATP-dependent interferon-responsive (ADIR) gene
    Cornelis A M van Bergen
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Blood 109:4089-96. 2007
    ..In conclusion, the novel mHag LB-ADIR-1F may be a suitable target for cellular immunotherapy when applied under controlled circumstances...
  6. ncbi request reprint Efficiency of T-cell receptor expression in dual-specific T cells is controlled by the intrinsic qualities of the TCR chains within the TCR-CD3 complex
    Mirjam H M Heemskerk
    Department of Hematology, Leiden University Medical Center, C2 R, PO Box 9600, 2300 RC Leiden, The Netherlands
    Blood 109:235-43. 2007
    ..In conclusion, introduced, endogenous, and chimeric TCRs compete for cell-surface expression in favor of the TCR-CD3 complex with best-pairing properties...
  7. ncbi request reprint Functional analysis of killer Ig-like receptor-expressing cytomegalovirus-specific CD8+ T cells
    Lars T van der Veken
    Department of Hematology, Laboratory of Experimental Hematology, Leiden University Medical Center, Leiden, The Netherlands
    J Immunol 182:92-101. 2009
    ..We observed no diminished general proliferative capacity and therefore we conclude that these T cells do not represent end-stage dysfunctional T cells...
  8. ncbi request reprint Early detection and rapid isolation of leukemia-reactive donor T cells for adoptive transfer using the IFN-gamma secretion assay
    Inge Jedema
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Clin Cancer Res 13:636-43. 2007
    ..The purpose of this study was to develop a new, broadly applicable strategy for the efficient generation and isolation of leukemia-reactive T cells with a better probability to survive and expand in vivo...
  9. doi request reprint Identification of 4 new HLA-DR-restricted minor histocompatibility antigens as hematopoietic targets in antitumor immunity
    Anita N Stumpf
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Blood 114:3684-92. 2009
    ..The selective recognition of hematopoietic cells as well as the balanced population frequencies and common HLA-DR restriction elements make the novel mHags possible targets for development of immunotherapeutic strategies...
  10. pmc Donor-derived mesenchymal stem cells are immunogenic in an allogeneic host and stimulate donor graft rejection in a nonmyeloablative setting
    Alma J Nauta
    Laboratory of Experimental Hematology, Department of Hematology, D2 P, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
    Blood 108:2114-20. 2006
    ....
  11. ncbi request reprint Reduced-intensity conditioning allogeneic stem cell transplantation with donor T-cell depletion using alemtuzumab added to the graft ('Campath in the bag')
    Peter A von dem Borne
    Department of Hematology C2 R, Leiden University Medical Center, Leiden, The Netherlands
    Curr Opin Oncol 21:S27-9. 2009
    ..AlloSCT with RIC and alemtuzumab-induced T-cell depletion offers a suitable platform for the investigation of novel cellular immunotherapy...
  12. doi request reprint Detailed analysis of IFNg response upon activation permits efficient isolation of cytomegalovirus-specific CD8+ T cells for adoptive immunotherapy
    Maarten L Zandvliet
    Department of Clinical Pharmacy and Toxicology, Leiden University Medical Center, Leiden, The Netherlands
    J Immunother 32:513-23. 2009
    ....
  13. pmc Identification of phosphatidylinositol 4-kinase type II beta as HLA class II-restricted target in graft versus leukemia reactivity
    Marieke Griffioen
    Departments of Hematology and Immunohematology and Blood Transfusion, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    Proc Natl Acad Sci U S A 105:3837-42. 2008
    ..We hypothesize that LB-PI4K2B-1S-specific CD4(+) T cells contributed to the antitumor response by both directly eliminating malignant cells as effector cells and stimulating CD8(+) T cell immunity as helper cells...
  14. pmc Serpina1 is a potent inhibitor of IL-8-induced hematopoietic stem cell mobilization
    Melissa van Pel
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Proc Natl Acad Sci U S A 103:1469-74. 2006
    ..Also, we hypothesize that cytokine-induced HSC/HPC mobilization is determined by a critical balance between serine proteases and serine protease inhibitors...
  15. ncbi request reprint Enhanced engraftment of umbilical cord blood-derived stem cells in NOD/SCID mice by cotransplantation of a second unrelated cord blood unit
    Alma J Nauta
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Exp Hematol 33:1249-56. 2005
    ..However, the low cell dose adversely affects hematopoietic recovery and therefore limits application of UCB transplantation in adults. Transplantation of multiple UCB units could be a strategy to overcome cell dose limitations...
  16. ncbi request reprint Repeated hematopoietic stem and progenitor cell mobilization without depletion of the bone marrow stem and progenitor cell pool in mice after repeated administration of recombinant murine G-CSF
    Evert Jan F M de Kruijf
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Hum Immunol 68:368-74. 2007
    ..The number of CFU-GM per femur did not significantly change for up to 12 cycles. We conclude that repeated administration of G-CSF does not lead to depletion of the bone marrow HSC/HPC pool...
  17. ncbi request reprint Responses to donor lymphocyte infusion for acute lymphoblastic leukemia may be determined by both qualitative and quantitative limitations of antileukemic T-cell responses as observed in an animal model for human leukemia
    Bart A Nijmeijer
    Leiden University Medical Center, Leiden, The Netherlands
    Exp Hematol 33:1172-81. 2005
    ..To allow identification of the causes of this failure, we established an animal model of DLI in human ALL...
  18. ncbi request reprint Up-regulated expression in nonhematopoietic tissues of the BCL2A1-derived minor histocompatibility antigens in response to inflammatory cytokines: relevance for allogeneic immunotherapy of leukemia
    Freke M Kloosterboer
    Department of Hematology, Leiden University Medical Center, C2 R, PO Box 9600, 2300 RC Leiden, The Netherlands
    Blood 106:3955-7. 2005
    ..Thus, the role of BCL2A1-specific T cells in differential induction of GVL reactivity and GVHD may depend on the presence of inflammatory responses that may occur during GVHD...
  19. doi request reprint Co-ordinated isolation of CD8(+) and CD4(+) T cells recognizing a broad repertoire of cytomegalovirus pp65 and IE1 epitopes for highly specific adoptive immunotherapy
    Maarten L Zandvliet
    Department of Clinical Pharmacy and Toxicology, Leiden University Medical Center, Leiden, The Netherlands
    Cytotherapy 12:933-44. 2010
    ....
  20. ncbi request reprint Identification of the angiogenic endothelial-cell growth factor-1/thymidine phosphorylase as a potential target for immunotherapy of cancer
    Elisabeth H Slager
    Department of Hematology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Blood 107:4954-60. 2006
    ..Because solid tumors expressing ECGF-1 could also be lysed by the CTL, ECGF-1 is an interesting target for immunotherapy of both hematologic and solid tumors...
  21. ncbi request reprint Redirection of antileukemic reactivity of peripheral T lymphocytes using gene transfer of minor histocompatibility antigen HA-2-specific T-cell receptor complexes expressing a conserved alpha joining region
    Mirjam H M Heemskerk
    Department of Hematology, Leiden University Medical Center, C2 R, PO Box 9600, 2300 RC Leiden, The Netherlands
    Blood 102:3530-40. 2003
    ....
  22. ncbi request reprint Inactivation of deoxycytidine kinase and overexpression of P-glycoprotein in AraC and daunorubicin double resistant leukemic cell lines
    Marjan J T Veuger
    Laboratory of Experimental Hematology, C2 R, Leiden University Medical Center, Department of Hematology, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands
    Leuk Res 27:445-53. 2003
    ..Our data implicate that similar resistance mechanisms like inactivation of dCK coincided by alternatively spliced dCK forms and overexpression of Pgp are induced in single-as well as in double resistant leukemic cell lines...
  23. pmc Generation and administration of HA-1-specific T-cell lines for the treatment of patients with relapsed leukemia after allogeneic stem cell transplantation: a pilot study
    Pauline Meij
    Department of Clinical Pharmacy and Toxicology, L0 P, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Haematologica 97:1205-8. 2012
    ..No toxicity after infusion was observed. Although in one patient, during a period of stable disease, HA-1-specific T cells could be detected in the peripheral blood and bone marrow, these patients had no clear clinical response...
  24. ncbi request reprint Mesenchymal stem cells inhibit generation and function of both CD34+-derived and monocyte-derived dendritic cells
    Alma J Nauta
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    J Immunol 177:2080-7. 2006
    ....
  25. doi request reprint High-throughput characterization of 10 new minor histocompatibility antigens by whole genome association scanning
    Cornelis A M van Bergen
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Cancer Res 70:9073-83. 2010
    ..Using tetramers, distinct populations of MiHA-specific CD8+ T cells were detected, demonstrating that our WGAs strategy allows high-throughput discovery of relevant targets in antitumor immunity after allo-SCT...
  26. ncbi request reprint Alpha-interferon with very-low-dose donor lymphocyte infusion for hematologic or cytogenetic relapse of chronic myeloid leukemia induces rapid and durable complete remissions and is associated with acceptable graft-versus-host disease
    Eduardus F M Posthuma
    Department of Hematology, Leiden University Medical Center, The Netherlands
    Biol Blood Marrow Transplant 10:204-12. 2004
    ..For patients with a molecular relapse, very-low-dose DLI alone is sufficient to induce molecular remissions in most patients and is associated with limited GVHD...
  27. ncbi request reprint Kinetic preservation of dual specificity of coprogrammed minor histocompatibility antigen-reactive virus-specific T cells
    Marleen M van Loenen
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Cancer Res 69:2034-41. 2009
    ..These data imply persistence of TCR-transferred virus-specific T cells with both antileukemic and antivirus reactivity in vivo...
  28. ncbi request reprint Differential activation of the death receptor pathway in human target cells induced by cytotoxic T lymphocytes showing different kinetics of killing
    Jeltje F de Vries
    Department of Hematology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Haematologica 92:1671-8. 2007
    ..Controversy exists whether, in addition to PFN/GrB-mediated apoptosis, death receptor-induced apoptosis contributes to the elimination of human tumor cells by CTL...
  29. ncbi request reprint Reduced stem cell mobilization in mice receiving antibiotic modulation of the intestinal flora: involvement of endotoxins as cofactors in mobilization
    Gerjo A Velders
    Laboratory of Experimental Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Blood 103:340-6. 2004
    ..1, P <.01). Our results indicate that endotoxins serve as cofactors in cytokine-induced mobilization. Modification of the endotoxin content by antibiotic treatment may affect the yield of cytokine-induced mobilization...
  30. doi request reprint Genetic engineering of virus-specific T cells with T-cell receptors recognizing minor histocompatibility antigens for clinical application
    Marieke Griffioen
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Haematologica 93:1535-43. 2008
    ..The purpose of this study was to develop an optimal T-cell receptors-encoding multi-cistronic retroviral vector and an efficient method for generating T-cell receptors-engineered virus-specific T cells...
  31. ncbi request reprint Phase I/II feasibility study evaluating the generation of leukemia-reactive cytotoxic T lymphocyte lines for treatment of patients with relapsed leukemia after allogeneic stem cell transplantation
    Erik Marijt
    Department of Hematology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Haematologica 92:72-80. 2007
    ..The aim of this study phase I/II study was to assess the feasibility of large-scale in vitro generation of leukemia-reactive CTL for clinical use...
  32. ncbi request reprint Outcomes after myeloablative unrelated donor stem cell transplantation using both in vitro and in vivo T-cell depletion with alemtuzumab
    Peter A von dem Borne
    Dept of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Haematologica 91:1559-62. 2006
    ....
  33. ncbi request reprint Long-term mixed chimerism after immunologic conditioning and MHC-mismatched stem-cell transplantation is dependent on NK-cell tolerance
    Geert Westerhuis
    Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands
    Blood 106:2215-20. 2005
    ..These data demonstrate the importance of NK cells in the rejection of MHC-mismatched hematopoietic cells and may explain the high numbers of bone marrow cells required for transplantation over MHC barriers...
  34. pmc Hematopoiesis-restricted minor histocompatibility antigens HA-1- or HA-2-specific T cells can induce complete remissions of relapsed leukemia
    W A Erik Marijt
    Department of Hematology, Leiden University Medical Center, PO Box 9600, 2300 RC, Leiden, The Netherlands
    Proc Natl Acad Sci U S A 100:2742-7. 2003
    ..This finding implies that in vitro generated HA-1- and HA-2-specific cytotoxic T lymphocytes could be used as adoptive immunotherapy to treat hematological malignancies relapsing after alloSCT...
  35. ncbi request reprint Enhancement of G-CSF-induced stem cell mobilization by antibodies against the beta 2 integrins LFA-1 and Mac-1
    Gerjo A Velders
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, The Netherlands
    Blood 100:327-33. 2002
    ..Enhancement of mobilization was not observed in LFA-1-deficient (CD11a) mice, indicating that activated cells expressing LFA-1 mediate the synergistic effect, rather than LFA-1-mediated adhesion...
  36. ncbi request reprint Minimal GVHD following in-vitro T cell-depleted allogeneic stem cell transplantation with reduced-intensity conditioning allowing subsequent infusions of donor lymphocytes in patients with hematological malignancies and solid tumors
    Renee M Y Barge
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Exp Hematol 31:865-72. 2003
    ..To reduce the incidence of GVHD, we treated a group of high-risk patients with a reduced-intensity conditioning regimen followed by in vitro T-cell-depleted alloSCT using Campath 1-H incubation...
  37. ncbi request reprint Proteosomal degradation of BCR/ABL protein can generate an HLA-A*0301-restricted peptide, but high-avidity T cells recognizing this leukemia-specific antigen were not demonstrated
    Eduardus F M Posthuma
    Dept of Hematology, Leiden, University Medical Center, PO Box 9600, 2300 RC, The Netherlands
    Haematologica 89:1062-71. 2004
    ..We analyzed the existence of high-avidity T cells recognizing endogenously processed BCR/ABL-specific proteins...
  38. doi request reprint Higher incidence of relapse with peripheral blood rather than marrow as a source of stem cells in adults with acute myelocytic leukemia autografted during the first remission
    Norbert Claude Gorin
    Hopital Saint Antoine, Department of Hematology, Paris, France
    J Clin Oncol 27:3987-93. 2009
    ..In acute myelocytic leukemia (AML), for patients who receive transplants during first complete remission (CR1), no prospective randomized study has compared relapse incidence (RI) to cell source...
  39. ncbi request reprint Homeostasis of telomere length rather than telomere shortening after allogeneic peripheral blood stem cell transplantation
    Helene Roelofs
    Department of Hematology, Leiden University Medical Center, The Netherlands
    Blood 101:358-62. 2003
    ....
  40. pmc Retroviral transfer of human CD20 as a suicide gene for adoptive T-cell therapy
    Marieke Griffioen
    Department of Hematology, Laboratory of Experimental Hematology, Leiden University Medical Center, Albinusdreef 2, Leiden, The Netherlands
    Haematologica 94:1316-20. 2009
    ..The data support the broad value of CD20 as safety switch in adoptive T-cell therapy...
  41. ncbi request reprint Allogeneic stem cell transplantation in acute lymphoblastic leukemia and non-Hodgkin's lymphoma for patients <or=50 years old in first complete remission: results of the EORTC ALL-3 trial
    Boris Labar
    Department of Hematology, University Hospital Center Rebro, Zagreb, Croatia
    Haematologica 89:809-17. 2004
    ..In the EORTC ALL-3 trial, the efficacy of allogeneic transplantation was compared with that of autologous marrow transplantation and maintenance chemotherapy in patients <or= 50 years who reached CR...
  42. pmc Reprogramming of virus-specific T cells into leukemia-reactive T cells using T cell receptor gene transfer
    Mirjam H M Heemskerk
    Department of Hematology, Leiden University Medical Center, C2 R, P O Box 9600, 2300 RC, Netherlands
    J Exp Med 199:885-94. 2004
    ....
  43. ncbi request reprint Primary allogeneic T-cell responses against mantle cell lymphoma antigen-presenting cells for adoptive immunotherapy after stem cell transplantation
    Mels Hoogendoorn
    Department of Hematology, Laboratory of Experimental Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Clin Cancer Res 11:5310-8. 2005
    ..The purpose of this study was to modify MCL cells into antigen-presenting cells (APC) and to use these MCL-APCs to induce allogeneic MCL-reactive T-cell responses...
  44. doi request reprint HLA-DPB1 mismatching results in the generation of a full repertoire of HLA-DPB1-specific CD4+ T cell responses showing immunogenicity of all HLA-DPB1 alleles
    Caroline E Rutten
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Biol Blood Marrow Transplant 16:1282-92. 2010
    ..We confirm previously described patterns in crossrecognition, and demonstrate that a high degree in similarity between HLA-DPB1 molecules is predictive for crossrecognition, but not for immunogenicity...
  45. ncbi request reprint Deoxycytidine kinase expression and activity in patients with resistant versus sensitive acute myeloid leukemia
    Marjan J T Veuger
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Eur J Haematol 69:171-8. 2002
    ..These data implicate that in our patient group of refractory or relapsed AML cases, alterations in dCK expression and/or activity cannot explain unresponsiveness to chemotherapy including AraC...
  46. ncbi request reprint Mesenchymal stem cells promote engraftment of human umbilical cord blood-derived CD34(+) cells in NOD/SCID mice
    Willy A Noort
    Department of Hematology, Leiden University Medical Center, Leiden University Medical Center, The Netherlands
    Exp Hematol 30:870-8. 2002
    ..In cotransplantation experiments, we examined the homing of MSC as well as the effect on engraftment of human umbilical cord blood (UCB)-derived CD34(+) cells in NOD/SCID mice...
  47. ncbi request reprint Differential maturation of megakaryocyte progenitor cells from cord blood and mobilized peripheral blood
    Laurus F Schipper
    Blood Bank Leiden Haaglanden, Leiden, The Netherlands
    Exp Hematol 31:324-30. 2003
    ..We compared the hematopoietic composition of these grafts and determined the distribution of mature and immature megakaryocyte progenitor cells in cord blood and mobilized peripheral blood and their in vitro kinetic behavior...
  48. ncbi request reprint Alphabeta T-cell receptor engineered gammadelta T cells mediate effective antileukemic reactivity
    Lars T van der Veken
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Cancer Res 66:3331-7. 2006
    ..These data show that transfer of alphabetaTCRs to gammadelta T cells generated potent effector cells for immunotherapy of leukemia, without the expression of potentially hazardous mixed TCR dimers...
  49. ncbi request reprint Minor histocompatibility antigens as targets of cellular immunotherapy in leukaemia
    J H Frederik Falkenburg
    Department of Hematology, Leiden University Medical Centre, P O Box 9600, C2 R, 2300 RC Leiden, The Netherlands
    Best Pract Res Clin Haematol 17:415-25. 2004
    ....
  50. doi request reprint Sequential combination of gemtuzumab ozogamicin and standard chemotherapy in older patients with newly diagnosed acute myeloid leukemia: results of a randomized phase III trial by the EORTC and GIMEMA consortium (AML-17)
    Sergio Amadori
    Sergio Amadori and Adriano Venditti, Tor Vergata University Hospital Paolo De Fabritiis, St Eugenio Hospital Roberto Latagliata, University Sapienza Paola Fazi and Marco Vignetti, Gruppo Italiano Malattie Ematologiche dell Adulto, Roma Franca Falzetti, University Hospital, Perugia Domenico Magro, Pugliese Hospital, Catanzaro Giorgina Specchia, University Hospital, Bari, Italy Stefan Suciu and Matthias Karrasch, European Organisation for Research and Treatment of Cancer, Brussels Dominik Selleslag, Algemeen Ziekenhuis St Jan, Brugge Zwi Berneman, University Hospital, Antwerp, Belgium Roberto Stasi, St George s Hospital, London, United Kingdom Helmut R Salih, University Hospital, Tubingen Anthony D Ho, University Hospital, Heidelberg Michael Lübbert, Albert Ludwigs University, Freiburg, Germany Petra Muus and Theo de Witte, Radboud University Medical Centre, Nijmegen Constantijn J M Halkes and Roel Willemze, University Medical Center, Leiden, the Netherlands Xavier Thomas, Edouard Herriot Hospital, Lyon Jean Pierre Marie, St Antoine Hospital, Paris, Germany
    J Clin Oncol 31:4424-30. 2013
    ..This randomized trial evaluated the efficacy and toxicity of sequential gemtuzumab ozogamicin (GO) and standard chemotherapy in older patients with newly diagnosed acute myeloid leukemia (AML)...
  51. doi request reprint Long-term culture of primary human lymphoblastic leukemia cells in the absence of serum or hematopoietic growth factors
    Bart A Nijmeijer
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Exp Hematol 37:376-85. 2009
    ..Available in vitro culturing protocols do not support long-term proliferation of primary cells. We therefore aimed to develop a culture system that allows long-term proliferation of primary human B-lineage lymphoblastic leukemia...
  52. ncbi request reprint An animal model for human cellular immunotherapy: specific eradication of human acute lymphoblastic leukemia by cytotoxic T lymphocytes in NOD/scid mice
    Bart A Nijmeijer
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Blood 100:654-60. 2002
    ..Ex vivo analysis showed decreased reactivity as compared to the moment of infusion. We therefore conclude that the model can be used to explore the requirements for optimal in vivo efficacy of in vitro- generated CTLs...
  53. pmc Combined CD8+ and CD4+ adenovirus hexon-specific T cells associated with viral clearance after stem cell transplantation as treatment for adenovirus infection
    Maarten L Zandvliet
    Department of Clinical Pharmacy and Toxicology, Leiden University Medical Center, Leiden, The Netherlands
    Haematologica 95:1943-51. 2010
    ....
  54. ncbi request reprint Minor histocompatibility antigens in human stem cell transplantation
    J H Frederik Falkenburg
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Exp Hematol 31:743-51. 2003
    ..By in vitro generation of T-cell responses against defined mHags, the efficacy and specificity of cellular immunotherapy against hematologic malignancies in the context of allogeneic transplantation may be improved...
  55. ncbi request reprint Cytokine-dependent proliferation of human CD34+ progenitor cells in the absence of serum is suppressed by their progeny's production of serine proteinases
    Henriette M Goselink
    Laboratory of Experimental Hematology, Department of Hematology, C2 R, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Stem Cells 24:299-306. 2006
    ....
  56. ncbi request reprint Acute myeloid leukemia cells in G0 phase of the cell cycle that are unresponsive to conventional chemotherapy are sensitive to treatment with granulocyte-macrophage colony-stimulating factor/diphtheria toxin fusion proteins
    Inge Jedema
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Exp Hematol 32:188-94. 2004
    ..In this study we manipulated the cell cycle and proliferative status of leukemic cells, explored the effect on sensitivity to DT, and determined the ability of DT388GMCSF fusion proteins to activate and subsequently kill leukemic cells...
  57. ncbi request reprint Long-term follow-up of recipients of allogeneic bone marrow grafts reveals no progressive telomere shortening and provides no evidence for haematopoietic stem cell exhaustion
    Elmar S D de Pauw
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    Br J Haematol 116:491-6. 2002
    ..6 kb shorter than those from donors (P = 0.01). The latter may be related to the reported rapid peripheral expansion of memory T cells immediately after transplantation...
  58. doi request reprint Identical outcome after autologous or allogeneic genoidentical hematopoietic stem-cell transplantation in first remission of acute myelocytic leukemia carrying inversion 16 or t(8;21): a retrospective study from the European Cooperative Group for Blood an
    Norbert Claude Gorin
    Department of Hematology, Hopital Saint Antoine and Université Pierre et Marie Curie UPMC, 184 rue du Faubourg Saint Antoine, Cedex 12 Paris, France
    J Clin Oncol 26:3183-8. 2008
    ..They presently do not undergo transplantation in first remission (CR1), but there is concern about late relapses...
  59. ncbi request reprint The DBY gene codes for an HLA-DQ5-restricted human male-specific minor histocompatibility antigen involved in graft-versus-host disease
    Mario H J Vogt
    Department of Hematology, Leiden University Medical Center, The Netherlands
    Blood 99:3027-32. 2002
    ..Although the epitope differed by 3 amino acids from its X-homolog DBX, only 2 polymorphisms were shown to be essential for recognition by the CTL clone...
  60. ncbi request reprint New CFSE-based assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell population
    Inge Jedema
    Department of Hematology, Leiden University Medical Center, The Netherlands
    Blood 103:2677-82. 2004
    ....
  61. pmc Identification of varicella-zoster virus-specific CD8 T cells in patients after T-cell-depleted allogeneic stem cell transplantation
    Pim L J van der Heiden
    Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    J Virol 83:7361-4. 2009
    ..In 42% of HLA-A2 patients following VZV reactivation, these IE62-ALW-A2 T cells could be detected ex vivo...
  62. ncbi request reprint The mechanisms of Ara-C-induced apoptosis of resting B-chronic lymphocytic leukemia cells
    Jeltje F de Vries
    Department of Hematology, Leiden University Medical Center, P O Box 9600 2300 RC Leiden, The Netherlands
    Haematologica 91:912-9. 2006
    ..In this study we analyzed which cellular processes were involved in Ara-C-mediated killing of G(O)-B-CLL cells...
  63. ncbi request reprint Functional role of alternatively spliced deoxycytidine kinase in sensitivity to cytarabine of acute myeloid leukemic cells
    Marjan J T Veuger
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, The Netherlands
    Blood 99:1373-80. 2002
    ..However, a cell expressing alternatively spliced dCK forms that has lost wt dCK expression is resistant to the cytotoxic effects of AraC...
  64. ncbi request reprint Magnetic resonance imaging of fetal bone marrow for quantitative definition of the human fetal stem cell compartment
    Jannine Wilpshaar
    Department of Hematology, Leiden University Medical Center, The Netherlands
    Blood 100:451-7. 2002
    ..Combining MRI and cell counts provides a method to quantify specific cell populations in fetal compartments. This study may enable better evaluation of fetal diagnostics and therapies...
  65. ncbi request reprint Loss or downregulation of HLA class I expression at the allelic level in acute leukemia is infrequent but functionally relevant, and can be restored by interferon
    Rolf E Brouwer
    Laboratory of Experimental Hematology, Department of Hematology, Leiden, The Netherlands
    Hum Immunol 63:200-10. 2002
    ..HLA downregulation was reversible and T-cell recognition could be restored by alpha- or gamma-interferon...
  66. pmc Neutrophils are indispensable for hematopoietic stem cell mobilization induced by interleukin-8 in mice
    Johannes F M Pruijt
    Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
    Proc Natl Acad Sci U S A 99:6228-33. 2002
    ..These data demonstrate that IL-8-induced mobilization of HPCs requires the in vivo activation of circulating PMNs...
  67. pmc Use of glycosylated recombinant human G-CSF (lenograstim) during and/or after induction chemotherapy in patients 61 years of age and older with acute myeloid leukemia: final results of AML-13, a randomized phase-3 study
    Sergio Amadori
    Department of Hematology, University Tor Vergata, St Eugenio Hospital, P le dell Umanesimo, 10, 00144 Rome, Italy
    Blood 106:27-34. 2005
    ..2 vs 29.7 days; P < .001). We conclude that although priming with G-CSF can improve the CR rate, the use of G-CSF during and/or after chemotherapy has no effect on the long-term outcome of AML in older patients...
  68. ncbi request reprint Allogeneic compared with autologous stem cell transplantation in the treatment of patients younger than 46 years with acute myeloid leukemia (AML) in first complete remission (CR1): an intention-to-treat analysis of the EORTC/GIMEMAAML-10 trial
    Stefan Suciu
    European Organization for Research and Treatment of Cancer Data Center, Brussels, Belgium
    Blood 102:1232-40. 2003
    ..In younger patients (15-35 years), the difference was more pronounced. The strategy to perform early allo-SCT led to better overall results than auto-SCT, especially for younger patients or those with bad/very bad risk cytogenetics...
  69. ncbi request reprint Hematopoietic stem cell transplantation for de novo erythroleukemia: a study of the European Group for Blood and Marrow Transplantation (EBMT)
    Loic Fouillard
    Centre International Greffe de Moelle EBMT, Centre de Recherche Claude Bernard sur la Thérapie Cellulaire, Universite Pierre et Marie Curie Paris VI, 75571 Paris Cedex 12, France
    Blood 100:3135-40. 2002
    ..By multivariate analysis, prognostic factors were graft-versus-host disease and age. This study represents the largest series of de novo EL treated with HSCT and shows that allogeneic HSCT is by far the most effective treatment...
  70. ncbi request reprint Sequential administration of gemtuzumab ozogamicin and conventional chemotherapy as first line therapy in elderly patients with acute myeloid leukemia: a phase II study (AML-15) of the EORTC and GIMEMA leukemia groups
    Sergio Amadori
    Department of Hematology, University Tor Vergata, St Eugenio Hospital, Rome, Italy
    Haematologica 89:950-6. 2004
    ..The aim of this collaborative trial was to assess the feasibility, safety, and antileukemic activity of administering GO followed by conventional chemotherapy as first line therapy in patients aged 61-75 years with AML...
  71. ncbi request reprint Revised recommendations of the International Working Group for Diagnosis, Standardization of Response Criteria, Treatment Outcomes, and Reporting Standards for Therapeutic Trials in Acute Myeloid Leukemia
    Bruce D Cheson
    Department of Hematology Oncology, Georgetown University, Lombardi Cancer Center, 3800 Reservoir Rd, NW, Washington, DC 20007, USA
    J Clin Oncol 21:4642-9. 2003
    ..Storage of viable blasts for correlative studies is important for future progress in the therapy of these disorders...
  72. ncbi request reprint Adult acute lymphoblastic leukaemia
    Renato Bassan
    Ospedali Riuniti, Bergamo, Italy
    Crit Rev Oncol Hematol 50:223-61. 2004
    ..Therapeutic progress may be obtainable through a careful integration of chemotherapy, stem cell transplantation, and the new targeted treatments with highly specific metabolic inhibitors and humanised monoclonal antibodies...
  73. ncbi request reprint The treatment of acute myeloid leukemia in first relapse: a comprehensive review of the literature
    Lance H Leopold
    Department of Clinical Research and Development, Wyeth Research, P O Box 42528, Philadelphia, PA 19101, USA
    Leuk Lymphoma 43:1715-27. 2002
    ..The CR2 rates were closely associated with age and duration of CR1. Therefore, considering the poor clinical outcomes of patients with AML in first relapse, improved therapies need to be developed...