A M H P van den Besselaar

Summary

Affiliation: Leiden University Medical Center
Country: The Netherlands

Publications

  1. ncbi request reprint Effect of magnesium contamination in evacuated blood collection tubes on the prothrombin time test and ISI calibration using recombinant human thromboplastin and different types of coagulometer
    A M H P van den Besselaar
    Hemostasis and Thrombosis Research Center, Department of Hematology, Leiden University Medical Center, C2 R, P O Box 9600, Leiden 2300 RC, The Netherlands
    Thromb Res 115:239-44. 2005
  2. doi request reprint Thromboplastin standards
    A M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Biologicals 38:430-6. 2010
  3. ncbi request reprint A comparative study of conventional versus new, magnesium-poor Vacutainer® Sodium Citrate blood collection tubes for determination of prothrombin time and INR
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands Electronic address
    Thromb Res 134:187-91. 2014
  4. ncbi request reprint Preparation of control blood for external quality assessment of point-of-care international normalized ratio testing in the Netherlands
    Anton M H P van den Besselaar
    From the Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Am J Clin Pathol 141:878-83. 2014
  5. ncbi request reprint Long-term stability of frozen pooled plasmas stored at -70°C, -40°C, and -20°C for prothrombin time and International Normalized Ratio (INR) assessment
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Thromb Res 131:349-51. 2013
  6. doi request reprint Point-of-care monitoring of vitamin K-antagonists: validation of CoaguChek XS test strips with International Standard thromboplastin
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Centre, Leiden, The Netherlands
    J Clin Pathol 65:1031-5. 2012
  7. doi request reprint Biological variation of INR in stable patients on long-term anticoagulation with warfarin
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Thromb Res 130:535-7. 2012
  8. doi request reprint Comparative study of blood collection tubes and thromboplastin reagents for correction of INR discrepancies: a proposal for maximum allowable magnesium contamination in sodium citrate anticoagulant solutions
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, The Netherlands
    Am J Clin Pathol 138:248-54. 2012
  9. doi request reprint Harmonization and external quality assessment of antithrombin activity assays
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Centre, Leiden, The Netherlands
    Thromb Res 129:187-91. 2012
  10. ncbi request reprint Magnesium and manganese ions accelerate tissue factor-induced coagulation independently of factor IX
    A M H P van den Besselaar
    Haemostasis and Thrombosis Research Center, Leiden University Medical Center, The Netherlands
    Blood Coagul Fibrinolysis 13:19-23. 2002

Detail Information

Publications41

  1. ncbi request reprint Effect of magnesium contamination in evacuated blood collection tubes on the prothrombin time test and ISI calibration using recombinant human thromboplastin and different types of coagulometer
    A M H P van den Besselaar
    Hemostasis and Thrombosis Research Center, Department of Hematology, Leiden University Medical Center, C2 R, P O Box 9600, Leiden 2300 RC, The Netherlands
    Thromb Res 115:239-44. 2005
    ..Magnesium chloride added to patients' blood (0.2 mmol/l) induced 2.3% reduction of the INR determined with rTF/95 and the manual technique...
  2. doi request reprint Thromboplastin standards
    A M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Biologicals 38:430-6. 2010
    ..In addition to monitoring of VKA, the PT is used for management of patients with chronic liver disease. Likewise, INR(liver) should be based on calibration with an IS using samples from patients with chronic liver disease...
  3. ncbi request reprint A comparative study of conventional versus new, magnesium-poor Vacutainer® Sodium Citrate blood collection tubes for determination of prothrombin time and INR
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands Electronic address
    Thromb Res 134:187-91. 2014
    ..The purpose of the present study was to compare PT and INR determined in specimens drawn with the new tubes to those drawn with the conventional tubes...
  4. ncbi request reprint Preparation of control blood for external quality assessment of point-of-care international normalized ratio testing in the Netherlands
    Anton M H P van den Besselaar
    From the Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Am J Clin Pathol 141:878-83. 2014
    ..The aim of this study was to prepare control blood for an external quality assessment scheme (EQAS) for international normalized ratio (INR) point-of-care testing (POCT) in the Netherlands and to assess the performance of the participants...
  5. ncbi request reprint Long-term stability of frozen pooled plasmas stored at -70°C, -40°C, and -20°C for prothrombin time and International Normalized Ratio (INR) assessment
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Thromb Res 131:349-51. 2013
    ..The purpose of the present study is to estimate the long-term stability of frozen pooled plasmas stored for at least three years...
  6. doi request reprint Point-of-care monitoring of vitamin K-antagonists: validation of CoaguChek XS test strips with International Standard thromboplastin
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Centre, Leiden, The Netherlands
    J Clin Pathol 65:1031-5. 2012
    ..We assessed the International Normalised Ratio (INR) differences between each of 51 new lots of test strips and the International Standard for thromboplastin rTF/95 or its successor rTF/09...
  7. doi request reprint Biological variation of INR in stable patients on long-term anticoagulation with warfarin
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Thromb Res 130:535-7. 2012
    ..5% CV and <2.25% CV, respectively. The biological variation and analytical performance goals have been derived using classic laboratory methods but should be applicable to point-of-care testing as well...
  8. doi request reprint Comparative study of blood collection tubes and thromboplastin reagents for correction of INR discrepancies: a proposal for maximum allowable magnesium contamination in sodium citrate anticoagulant solutions
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, The Netherlands
    Am J Clin Pathol 138:248-54. 2012
    ..INR discrepancies could be explained largely by this influence of blood collection tubes. The maximum allowable magnesium contamination in sodium citrate anticoagulant solutions should be less than 1 mmol/L...
  9. doi request reprint Harmonization and external quality assessment of antithrombin activity assays
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Centre, Leiden, The Netherlands
    Thromb Res 129:187-91. 2012
    ..The interlaboratory coefficient of variation (CV) of the antithrombin results for three test samples could be reduced from 6.9 - 13.2% (before harmonization) to 5.6 - 9.8% using the common calibrator...
  10. ncbi request reprint Magnesium and manganese ions accelerate tissue factor-induced coagulation independently of factor IX
    A M H P van den Besselaar
    Haemostasis and Thrombosis Research Center, Leiden University Medical Center, The Netherlands
    Blood Coagul Fibrinolysis 13:19-23. 2002
    ..From these experiments, it is inferred that activation of factor X by factor VII-TF can be accelerated by magnesium and manganese ions independently of factor IX...
  11. doi request reprint Lupus anticoagulant: case-based external quality assessment
    A M H P van den Besselaar
    Department of Thrombosis and Haemostasis, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands
    J Clin Pathol 62:731-4. 2009
    ..The purpose of the present study was to assess the performance of clinical laboratories in case-based EQA using the case history of a patient suffering from lupus erythematosus of the skin...
  12. ncbi request reprint Harmonisation of factor VIII:C assay results: study within the framework of the Dutch project 'Calibration 2000'
    Anton M H P van den Besselaar
    Department of Haematology, Haemostasis and Thrombosis Research Centre, Leiden University Medical Centre, Leiden, The Netherlands
    Br J Haematol 132:75-9. 2006
    ..We recommend that at least three different sample dilutions be used in each FVIII:C assay, in agreement with previous recommendations...
  13. doi request reprint Harmonization of fibrinogen assay results: study within the framework of the Dutch project 'Calibration 2000'
    A M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Centre, PO Box 9600, Leiden 2300 RC, The Netherlands
    Int J Lab Hematol 31:513-20. 2009
    ..3% using the routine measurements and 7.8% using the common calibrator. Despite the reduction in the overall CV, the bias between Clauss and PT-derived assay results in two coumarin test samples could not be eliminated...
  14. ncbi request reprint Influence of haematocrit on international normalised ratio (INR) differences between a whole blood point-of-care coagulation monitor and reference prothrombin time in plasma
    Anton M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Thromb Haemost 100:1181-4. 2008
    ..37 - 0.51, on the INR difference was not greater than approximately 10% for the combined data of the four strip lots. A bias of less than 10% seems to be acceptable clinically...
  15. ncbi request reprint Guidelines on preparation, certification, and use of certified plasmas for ISI calibration and INR determination
    A M H P van den Besselaar
    Hemostasis and Thrombosis Research Center, Department of Hematology, Leiden University Medical Center, The Netherlands
    J Thromb Haemost 2:1946-53. 2004
    ..Discrepant results obtained by users of certified plasmas should be reported to manufacturers or suppliers...
  16. ncbi request reprint Long-term stability and reproducibility of CoaguChek test strips
    Anton M H P van den Besselaar
    Haemostasis and Thrombosis Research Center, Department of Hematology, C2 R Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Thromb Haemost 93:1189-92. 2005
    ..58% to 3.36% (CV). The between-lot variation of the mean INR ranged from 3.2 to 4.5 % (CV). The over-all variation of single INR measurements, i.e. including between-lot and within-lot, ranged from 5.0 to 6.0 % (CV)...
  17. ncbi request reprint A comparison between capillary and venous blood international normalized ratio determinations in a portable prothrombin time device
    A M van den Besselaar
    Hemostasis and Thrombosis Research Center, Department of Hematology, Leiden University Medical Center, The Netherlands
    Blood Coagul Fibrinolysis 11:559-62. 2000
    ..8 and 2.8% for the strips with ISI 1.5 and 1.1, respectively. These deviations are clinically acceptable. It is concluded that capillary blood can be replaced by venous blood for the calibration of the CoaguChek PT system...
  18. ncbi request reprint A reassessment of the relationship between international reference preparations for human and rabbit thromboplastins
    A M H P van den Besselaar
    Haemostasis and Thrombosis Research Centre, Leiden University Medical Centre, Leiden, The Netherlands
    Thromb Haemost 88:459-61. 2002
    ..961 (between-lab SD 0.028) which was 2% greater than the historical value determined in 1995. There is no indication that the two IRP have deteriorated, but further monitoring of the calibration relationship is recommended...
  19. ncbi request reprint The influence of exogenous magnesium chloride on the apparent INR determined with human, rabbit, and bovine thromboplastin reagents
    Anton M H P van den Besselaar
    Haemostasis and Thrombosis Research Center, Department of Haematology, Leiden University Medical Center, Leiden, The Netherlands
    Thromb Haemost 89:43-7. 2003
    ..1 mmol/l magnesium in the blood was smaller than 10% in all patient samples. At 0.5 mmol/l magnesium in the blood, 10-35% of the patient samples had INR deviations greater than 10%, depending on the thromboplastin reagent used...
  20. ncbi request reprint Field study of lyophilized calibrant plasmas for fresh plasma INR determination
    A M H P van den Besselaar
    Haemostasis and Thrombosis Research Center, Department of Haematology, Leiden University Medical Center, The Netherlands
    Thromb Haemost 87:277-81. 2002
    ..In contrast, discrepant INR were obtained when "dissimilar" cross-species calibration procedures were used. The study was limited to 9 laboratories using the same type of coagulometer and one using a different type...
  21. ncbi request reprint Prothrombin time ratio is reduced by magnesium contamination in evacuated blood collection tubes
    A M van den Besselaar
    Department of Haematology, Leiden University Medical Center, The Netherlands
    Thromb Haemost 85:647-50. 2001
    ..e., in the absence of citrate. These results indicate that magnesium contamination can interfere with accurate INR determination in the control of oral anticoagulant therapy...
  22. ncbi request reprint A comparison of two sodium citrate concentrations in two evacuated blood collection systems for prothrombin time and ISI determination
    A M van den Besselaar
    Haemostasis and Thrombosis Research Center, Leiden University Medical Center, The Netherlands
    Thromb Haemost 84:664-7. 2000
    ..05) for most thromboplastin systems and amounted to 7%. ISI interchange between these glass and PET tubes could induce INR differences amounting to 14%, which could affect clinical dosage of oral anticoagulants...
  23. ncbi request reprint Monitoring of unfractionated heparin with the activated partial thromboplastin time: determination of therapeutic ranges
    A M H P van den Besselaar
    Department of Hematology, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    Thromb Res 107:235-40. 2002
    ..The present results show that, if anti-Xa concentrations of 0.29-0.47 IU/ml reflect the true therapeutic range, many laboratories do not use the proper therapeutic APTT range...
  24. doi request reprint Effect of prothrombin time outlier exclusion rules on ISI calibration in multicentre studies
    A M H P van den Besselaar
    Department of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Thromb Res 125:523-8. 2010
    ..It is concluded that the WHO rule for sample exclusion is appropriate for reliable ISI calibration...
  25. doi request reprint Variability of INR in patients on stable long-term treatment with phenprocoumon and acenocoumarol and implications for analytical quality requirements
    Johanna H H van Geest-Daalderop
    Thrombosis Service, Laboratory of Clinical Chemistry and Haematology, Jeroen Bosch Hospital, s Hertogenbosch, The Netherlands
    Thromb Haemost 102:588-92. 2009
    ..Desirable INR imprecision goals are <4.9% and <5.3% CV for monitoring of phenprocoumon and acenocoumarol, respectively. These goals were achieved using the aforesaid PT system...
  26. ncbi request reprint European Concerted Action on Anticoagulation (ECAA): multicentre international sensitivity index calibration of two types of point-of-care prothrombin time monitor systems
    L Poller
    ECAA Central Facility, School of Biological Sciences, The University of Manchester, Manchester, UK
    Br J Haematol 116:844-50. 2002
    ..In these circumstances, the modified WHO-type ISI calibration appeared to be satisfactory for the POCT whole-blood monitors...
  27. ncbi request reprint Oral anticoagulant monitoring by laboratory or near-patient testing: what a clinician should be aware of
    A Tripodi
    Bonomi Hemophilia and Thrombosis Centre, University and IRCCS Maggiore Hospital, Milan, Italy
    Semin Vasc Med 3:243-54. 2003
    ..Each center should maintain an appropriate scheme of internal and external quality control for the laboratory INR measurement as well as the individual point-of-care coagulation monitors used by the center and patients for self-testing...
  28. ncbi request reprint European Concerted Action on Anticoagulation--comparison of fresh plasma and whole blood multicentre ISI calibrations of CoaguChek Mini and TAS PT-NC whole blood prothrombin time point-of-care monitors
    L Poller
    European Concerted Action on Anticoagulation Central Facility, School of Biological Sciences, The University of Manchester, UK
    Thromb Haemost 87:859-66. 2002
    ..With the CoaguChek Mini system, the plasma calibration ISI can also be used to derive reliable INR with whole blood PT results. This was not possible with the TAS PT-NC system...
  29. ncbi request reprint European Concerted Action on Anticoagulation (ECAA). Minimum number of centres for reliable International Sensitivity Index calibration of CoaguChek and TAS point-of-care whole blood monitors
    L Poller
    ECAA Central Facility, School of Biological Sciences, The University of Manchester, 3 239 Stopford Building, M13 9PT, Manchester, UK
    Thromb Res 107:61-6. 2002
    ..The aim of the present study was to determine the number of centres required to give reliable ISI and International Normalised Ratio (INR) with these two POCT whole blood PT monitors...
  30. ncbi request reprint Definition of the International Normalized Ratio (INR) and its consequences for the calibration procedure of thromboplastin preparations: a rebuttal
    A M H P van den Besselaar
    J Thromb Haemost 2:1490-1; reply 1492-4. 2004
  31. ncbi request reprint International multicenter international sensitivity index (ISI) calibration of a new human tissue factor thromboplastin reagent derived from cultured human cells
    W P M Houdijk
    BioMerieux bv, Boxtel, The Netherlands
    J Thromb Haemost 2:266-70. 2004
    ..4% higher (P < 0.005). Considering the principle of 'like vs. like', the ISIs obtained by calibration against rTF/95 should be preferred...
  32. ncbi request reprint European Concerted Action on Anticoagulation (ECAA): an assessment of a method for ISI calibration of two whole blood point-of-care PT monitor systems based on lyophilized plasmas using whole blood equivalent PT
    L Poller
    ECAA Central Facility, School of Biological Sciences, The University of Manchester, Manchester, UK
    J Thromb Haemost 1:766-72. 2003
    ..With the CoaguChek Mini, the correction based on the 'line of equivalence' did not improve results but was not required with this system...
  33. pmc European Concerted Action on Anticoagulation. A multicentre calibration study of WHO international reference preparations for thromboplastin, rabbit (RBT/90) and human (rTF/95)
    L Poller
    ECAA Central Facility, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK
    J Clin Pathol 58:667-9. 2005
    ..At a theoretical INR of 3.0, the INR bias is 6.0%, and this is below the accepted 10% level of clinical relevance. Ongoing stability monitoring of World Health Organisation thromboplastin IRP is advised...
  34. pmc European Concerted Action on Anticoagulation (ECAA). An assessment of lyophilised plasmas for ISI calibration of CoaguChek and TAS whole blood prothrombin time monitors
    L Poller
    ECAA Central Facility, School of Biological Sciences, The University of Manchester, Manchester M13 9PT, UK
    J Clin Pathol 56:114-9. 2003
    ..The present European Concerted Action on Anticoagulation (ECAA) study aimed to assess the reliability of simpler ISI calibration using lyophilised plasma samples...
  35. ncbi request reprint Is there a need for replacement of the International Reference Preparation for Thromboplastin, Bovine, Combined (OBT/79)?
    A M H P van den Besselaar
    J Thromb Haemost 3:2365-6. 2005
  36. ncbi request reprint International collaborative study for the calibration of a proposed international standard for thromboplastin, rabbit, plain
    V Chantarangkul
    Department of Internal Medicine, Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, University and IRCCS Ospedale Maggiore, Mangiagalli and Regina Elena Foundation, Milan, Italy
    J Thromb Haemost 4:1339-45. 2006
    ....
  37. ncbi request reprint Comparison of local International Sensitivity Index calibration and 'Direct INR' methods in correction of locally reported International Normalized Ratios: an international study
    L Poller
    The European Concerted Action on Anticoagulation Central Facility, University of Manchester, Faculty of Life Sciences, Manchester, UK
    J Thromb Haemost 5:1002-9. 2007
    ....
  38. ncbi request reprint Effect of daptomycin on prothrombin time and the requirement for outlier exclusion in International Sensitivity Index calibration of thromboplastin
    A M H P van den Besselaar
    J Thromb Haemost 5:1975-6. 2007
  39. doi request reprint Continuation of the international standard thromboplastin (human, recombinant, plain) by means of a replacement reconstitution fluid
    A M H P van den Besselaar
    J Thromb Haemost 6:1042-3. 2008
  40. doi request reprint An international multicenter randomized study of computer-assisted oral anticoagulant dosage vs. medical staff dosage
    L Poller
    EAA Central Facility, Faculty of Life Sciences, University of Manchester, Manchester, UK
    J Thromb Haemost 6:935-43. 2008
    ..A multicenter clinical endpoint study has been performed to compare the safety and effectiveness of computer-assisted dosage with dosage by experienced medical staff at the same centers...
  41. ncbi request reprint European Concerted Action on Anticoagulation (ECAA): International Normalized Ratio variability of CoaguChek and TAS point-of-care testing whole blood prothrombin time monitors
    L Poller
    ECAA Central Facility, School of Biological Sciences, The University of Manchester, Manchester, UK
    Thromb Haemost 88:992-5. 2002
    ..On both brands of monitor, INR variability appeared to be due mainly to duplication differences rather than between-instrument variability on both normal and coumarin whole blood samples...