Research Topics
Genomes and Genes | Frank J T StaalSummaryAffiliation: Leiden University Medical Center Country: The Netherlands Publications
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Publications
Tales of the unexpected: tcf1 functions as a tumor suppressor for leukemiasFrank J T Staal
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands Electronic address
Immunity 37:761-3. 2012..In this issue of Immunity, Yu et al. (2012) report that Tcf1-deficient mice develop aggressive T cell lymphomas that are characterized by high Lef1 expression...- Wnt signaling in hematopoiesis: crucial factors for self-renewal, proliferation, and cell fate decisionsFrank J T Staal
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
J Cell Biochem 109:844-9. 2010.... - Stem cell self-renewal: lessons from bone marrow, gut and iPS toward clinical applicationsF J T Staal
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Leukemia 25:1095-102. 2011..Here, we review the basic principles of different SC systems, and on the basis of the experience with HSC-based SC therapy, provide recommendations for clinical application of emerging SC technologies... - Genome-wide expression analysis of paired diagnosis-relapse samples in ALL indicates involvement of pathways related to DNA replication, cell cycle and DNA repair, independent of immune phenotypeF J T Staal
Department of Immunology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands
Leukemia 24:491-9. 2010..Thus, about half of the relapses are due to the selection or emergence of a clone with deregulated expression of genes involved in pathways that regulate B-cell signaling, development, cell cycle, cellular division and replication... 
Human thymus contains multipotent progenitors with T/B lymphoid, myeloid, and erythroid lineage potentialFloor Weerkamp
Department of Immunology, Erasmus MC, University Medical Center Rotterdam, The Netherlands
Blood 107:3131-7. 2006..These cells resemble hematopoietic stem cells but, by analogy with murine thymocytes, apparently lack sufficient self-renewal capacity...- Wnt3a deficiency irreversibly impairs hematopoietic stem cell self-renewal and leads to defects in progenitor cell differentiationTiago C Luis
Department of Immunology, ErasmusMC, Erasmus University Medical Center, Rotterdam, The Netherlands
Blood 113:546-54. 2009..Our results show that Wnt3a signaling not only provides proliferative stimuli, such as for immature thymocytes, but also regulates cell fate decisions of HSC during hematopoiesis... - Combined TCRG and TCRA TREC analysis reveals increased peripheral T-lymphocyte but constant intra-thymic proliferative history upon ageingKim van der Weerd
Department of Immunology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands
Mol Immunol 53:302-12. 2013..This novel TREC assay approach could prove valuable in immune status monitoring in a variety of conditions... - Gene therapy: is IL2RG oncogenic in T-cell development?Karin Pike-Overzet
Department of Immunology, Erasmus University Medical Center, 3015 GE Rotterdam, The Netherlands
Nature 443:E5; discussion E6-7. 2006.... - Canonical wnt signaling regulates hematopoiesis in a dosage-dependent fashionTiago C Luis
Department of Immunology, Erasmus University Medical Center, The Netherlands
Cell Stem Cell 9:345-56. 2011..Differential, lineage-specific optimal Wnt dosages provide a unifying concept that explains the differences reported among inducible gain-of-function approaches, leading to either HSC expansion or depletion of the HSC pool... - T-sing progenitors to commitFloor Weerkamp
Department of Immunology, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
Trends Immunol 27:125-31. 2006..Here we review the perspectives of different researchers working both on murine and human T-cell development and argue that a true T-cell commitment factor might not be required because of the unique properties of the thymus... 
The nuclear effector of Wnt-signaling, Tcf1, functions as a T-cell-specific tumor suppressor for development of lymphomasMachteld M Tiemessen
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
PLoS Biol 10:e1001430. 2012..Thus, Tcf1 acts as a molecular switch between proliferative and repressive signals during T-lymphocyte development in the thymus...- Wnt signaling in the thymus is regulated by differential expression of intracellular signaling moleculesFloor Weerkamp
Department of Immunology, Erasmus MC, University Medical Center Rotterdam, The Netherlands
Proc Natl Acad Sci U S A 103:3322-6. 2006..Thus, responsiveness to developmental signals can be regulated by differential expression of intracellular mediators rather than by abundance of receptors or ligands... - Distinct monocyte gene-expression profiles in autoimmune diabetesRoos C Padmos
Department of Immunology, Erasmus MC, Rotterdam, The Netherlands
Diabetes 57:2768-73. 2008..Our hypothesis is that monocytes are distinctly activated/disturbed in different subforms of autoimmune diabetes... - Differential requirements for Wnt and Notch signaling in hematopoietic versus thymic nichesPaul P C Roozen
Department of Immunohematology and Blood Transfusion IHB, Leiden University Medical Center, Leiden, The Netherlands
Ann N Y Acad Sci 1266:78-93. 2012..Here, we review the differences and similarities between the thymic and hematopoietic niches, with particular focus on Wnt and Notch signals, as well as the latest insights into regulation of these developmentally important pathways... - New insights and unresolved issues regarding insertional mutagenesis in X-linked SCID gene therapyKarin Pike Overzet
Department of Immunology, Erasmus MC, Erasmus University Medical Center, Rotterdam, The Netherlands
Mol Ther 15:1910-6. 2007..Finally, we review some newly developed murine models that may have added value for gene therapy safety studies... - New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profilingWillem A Dik
Department of Immunology, Erasmus MC, 3015 GE Rotterdam, Netherlands
J Exp Med 201:1715-23. 2005..Our data demonstrate that a number of key events occur earlier than assumed previously; therefore, human T cell development is much more similar to murine T cell development than reported before... - Isolation of human and mouse hematopoietic stem cellsYuk Yin Ng
Department of Immunology, Erasmus University Medical Center, Rotterdam, The Netherlands
Methods Mol Biol 506:13-21. 2009..Here we described the most often used protocols and methods to isolate human and mouse HSC to high purity... - Novel insights into the development of T-cell acute lymphoblastic leukemiaFrank J T Staal
Department of Immunology, Erasmus MC, Dr Molewaterplein 50, Rotterdam, The Netherlands
Curr Hematol Malig Rep 2:176-82. 2007..In particular, the activating mutations in the Notch pathways are believed to occur in a large proportion of human T-ALL. These findings on genetic events open up new therapeutic possibilities... - Signaling pathways involved in the development of T-cell acute lymphoblastic leukemiaFrank J T Staal
Department of Immunology, ErasmusMC, Rotterdam, The Netherlands
Haematologica 93:493-7. 2008 - Ig gene rearrangement steps are initiated in early human precursor B cell subsets and correlate with specific transcription factor expressionMenno C van Zelm
Department of Immunology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands
J Immunol 175:5912-22. 2005.... - Wnt target genes identified by DNA microarrays in immature CD34+ thymocytes regulate proliferation and cell adhesionFrank J T Staal
Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
J Immunol 172:1099-108. 2004..In conclusion, Wnt signals mediate proliferation and cell adhesion, but not differentiation of the immature thymic progenitor pool... - WNT proteins: environmental factors regulating HSC fate in the nicheTiago C Luis
Department of Immunology, Erasmus MC, Erasmus University Medical Center, Rotterdam, The Netherlands
Ann N Y Acad Sci 1176:70-6. 2009..Our results show that while in the thymus Wnt3a provides cytokine-like, proliferative stimuli to developing thymocyte Wnt3a regulates cell fate decisions of FL HSC in a nonredundant way... - DNA microarray studies of hematopoietic subpopulationsKarin Pike-Overzet
Department of Immunology, Erasmus University Medical Center, Rotterdam, The Netherlands
Methods Mol Biol 506:403-21. 2009..Over the last decade, the combination of these purification techniques with microarray analysis has resulted in an enormous gain of knowledge of blood lineages and their development... - The effect of oligonucleotide microarray data pre-processing on the analysis of patient-cohort studiesRoel G W Verhaak
Department of Hematology, Erasmus Medical Center, Rotterdam, The Netherlands
BMC Bioinformatics 7:105. 2006..By focusing on practical applications of microarray-based research, this study provides insight into the relevance of pre-processing procedures to biology-oriented researchers... - The canonical Wnt signaling pathway plays an important role in lymphopoiesis and hematopoiesisFrank J T Staal
Department of Immunology, Erasmus University Medical Center, Rotterdam, The Netherlands
Eur J Immunol 38:1788-94. 2008..In this perspective, we provide a broad background to the field with a discussion of the available data and create a framework within which the available and future studies may be evaluated... - Induced pluripotent stem cells and severe combined immunodeficiency: merely disease modeling or potentially a novel cure?Harald Mikkers
Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
Pediatr Res 71:427-32. 2012..Here, we review these recent advances in the field from an efficacy and safety point of view... - Morbidly obese human subjects have increased peripheral blood CD4+ T cells with skewing toward a Treg- and Th2-dominated phenotypeKim van der Weerd
1Department of Immunology, Erasmus University Medical Center, Rotterdam, The Netherlands
Diabetes 61:401-8. 2012..This is associated with increased CD4+ T cells, with skewing toward a Treg- and Th2-dominated phenotype, suggesting a more anti-inflammatory set point... - Age-related changes in the cellular composition of the thymus in childrenFloor Weerkamp
Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
J Allergy Clin Immunol 115:834-40. 2005..CONCLUSION: We conclude that T-cell development in children is a dynamic process, answering the demands of a maturing and expanding immune system... - Integrated transcript and genome analyses reveal NKX2-1 and MEF2C as potential oncogenes in T cell acute lymphoblastic leukemiaIrene Homminga
Department of Pediatric Oncology Hematology, Erasmus MC Sophia Children s Hospital, Rotterdam, The Netherlands
Cancer Cell 19:484-97. 2011..We propose NKX2-1, NKX2-2, and MEF2C as T-ALL oncogenes that are activated by various rearrangements... - WNT signalling and haematopoiesis: a WNT-WNT situationFrank J T Staal
Department of Immunology, Room Ee 838, Erasmus Medical Center, 3015GE Rotterdam, The Netherlands
Nat Rev Immunol 5:21-30. 2005.... - WNT signalling in the immune system: WNT is spreading its wingsFrank J T Staal
Department of Immunology, Erasmus University Medical Center, 3015 GE, Rotterdam, The Netherlands
Nat Rev Immunol 8:581-93. 2008..WNT signalling is therefore subject to strict molecular control, and dysregulated WNT signalling is implicated in the development of haematological malignancies... - Gene expression profiling in acute lymphoblastic leukemia (ALL)Frank J T Staal
Department of Immunology, Erasmus University Medical Center, Rotterdam, The Netherlands
Lab Hematol 10:178-81. 2004 - Selective in vitro expansion and efficient retroviral transduction of human CD34+ CD38- haematopoietic stem cellsYuk Yin Ng
Departments of Hematology and Immunology, University Medical Center, Utrecht, The Netherlands
Br J Haematol 117:226-37. 2002.... - Profiling of antibody production against xenograft-released proteins by protein microarrays discovers prostate cancer markersFlip H Jansen
Department of Urology, Erasmus MC, 3000 CA Rotterdam, The Netherlands
J Proteome Res 11:728-35. 2012..In conclusion, this novel method allows for the identification of low abundant cancer-derived serum proteins, circumventing dynamic range and host-response issues in standard patient cohort proteomics comparisons... - A discriminating messenger RNA signature for bipolar disorder formed by an aberrant expression of inflammatory genes in monocytesRoos C Padmos
Department of Immunology, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
Arch Gen Psychiatry 65:395-407. 2008..Mood disturbances are associated with an activated inflammatory response system... - Endothelial progenitor cell dysfunction in type 1 diabetes: another consequence of oxidative stress?Cindy J M Loomans
Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands
Antioxid Redox Signal 7:1468-75. 2005..Finally, we discuss the potential implication of our findings for EPC-based therapies and the potential impact of pharmacological interventions on the vascular regenerative capacity of EPC...