Threes G M Smijs
Affiliation: Leiden University Medical Center
Country: The Netherlands
Smijs T, Pavel S, Talebi M, Bouwstra J. Preclinical studies with 5,10,15-Tris(4-methylpyridinium)-20-phenyl-[21H,23H]-porphine trichloride for the photodynamic treatment of superficial mycoses caused by Trichophyton rubrum. Photochem Photobiol. 2009;85:733-9 pubmed publisher
..Therefore, PDT with 10 microm Sylsens B (formulation I) and 18 J/cm(2) UVA-1 could be considered as effective and safe. This offers the possibility to perform clinical studies in future. ..
Smijs T, van der Haas R, Lugtenburg J, Liu Y, de Jong R, Schuitmaker H. Photodynamic treatment of the dermatophyte Trichophyton rubrum and its microconidia with porphyrin photosensitizers. Photochem Photobiol. 2004;80:197-202 pubmed
..These results offer the ingredients for a future treatment of fungal infections, including those of the nail. ..
Smijs T, Bouwstra J, Talebi M, Pavel S. Investigation of conditions involved in the susceptibility of the dermatophyte Trichophyton rubrum to photodynamic treatment. J Antimicrob Chemother. 2007;60:750-9 pubmed
..Under these conditions, PDT with Sylsens B inactivates, initially via singlet oxygen, effectively the fungus in different fungal growth stages. ..
Smijs T, Mulder A, Pavel S, Onderwater J, Koerten H, Bouwstra J. Morphological changes of the dermatophyte Trichophyton rubrum after photodynamic treatment: a scanning electron microscopy study. Med Mycol. 2008;46:315-25 pubmed publisher
..In case of a non-lethal PDT, fungal re-growth started on the remnants of the treated mycelium. ..
Smijs T, Nivard M, Schuitmaker H. Development of a test system for mutagenicity of photosensitizers using Drosophila melanogaster. Photochem Photobiol. 2004;79:332-8 pubmed
..We demonstrated that TPP was mutagenic using the Photo-SMART. For hematoporphyrin, the results of the Photo-SMART indicate the absence of mutagenicity. ..
Smijs T, Bouwstra J, Schuitmaker H, Talebi M, Pavel S. A novel ex vivo skin model to study the susceptibility of the dermatophyte Trichophyton rubrum to photodynamic treatment in different growth phases. J Antimicrob Chemother. 2007;59:433-40 pubmed
..We conclude that T. rubrum is susceptible to PDT in a situation that mimics the clinical situation. The fungicidal effect of PDT on fungal spores is of particular importance. ..