Robert E Poelmann

Summary

Affiliation: Leiden University Medical Center
Country: The Netherlands

Publications

  1. ncbi The myth of ventrally emigrating neural tube (VENT) cells and their contribution to the developing cardiovascular system
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
    Anat Embryol (Berl) 206:327-33. 2003
  2. ncbi Expression patterns of Tgfbeta1-3 associate with myocardialisation of the outflow tract and the development of the epicardium and the fibrous heart skeleton
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 227:431-44. 2003
  3. ncbi Nuchal edema and venous-lymphatic phenotype disturbance in human fetuses and mouse embryos with aneuploidy
    Mireille N Bekker
    Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, The Netherlands
    J Soc Gynecol Investig 13:209-16. 2006
  4. ncbi The role of shear stress on ET-1, KLF2, and NOS-3 expression in the developing cardiovascular system of chicken embryos in a venous ligation model
    Bianca C W Groenendijk
    Department of Medical Biochemistry, Academic Medical Center, Amsterdam, The Netherlands
    Physiology (Bethesda) 22:380-9. 2007
  5. doi Podoplanin deficient mice show a RhoA-related hypoplasia of the sinus venosus myocardium including the sinoatrial node
    Edris A F Mahtab
    Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands
    Dev Dyn 238:183-93. 2009
  6. ncbi The neural crest is contiguous with the cardiac conduction system in the mouse embryo: a role in induction?
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, Wassenaarseweg 62, PO 9602, Leiden, The Netherlands
    Anat Embryol (Berl) 208:389-93. 2004
  7. pmc The development of the heart and microcirculation: role of shear stress
    Robert E Poelmann
    Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands
    Med Biol Eng Comput 46:479-84. 2008
  8. doi Cardiac development
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    ScientificWorldJournal 8:855-8. 2008
  9. ncbi Apoptosis as an instrument in cardiovascular development
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Birth Defects Res C Embryo Today 75:305-13. 2005
  10. ncbi Developmental coronary maturation is disturbed by aberrant cardiac vascular endothelial growth factor expression and Notch signalling
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, PO Box 9600, 2300 RC Leiden, The Netherlands
    Cardiovasc Res 78:366-75. 2008

Collaborators

Detail Information

Publications75

  1. ncbi The myth of ventrally emigrating neural tube (VENT) cells and their contribution to the developing cardiovascular system
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
    Anat Embryol (Berl) 206:327-33. 2003
    ..We conclude that there is no contribution of ventral neural tube cells to the developing cardiovascular system...
  2. ncbi Expression patterns of Tgfbeta1-3 associate with myocardialisation of the outflow tract and the development of the epicardium and the fibrous heart skeleton
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 227:431-44. 2003
    ..The information provided favors novel roles of Tgfbeta1-3 in epicardial development, of Tgfbeta2 in myocardialisation, and of Tgfbeta3 in differentiation of the fibrous structures of the heart...
  3. ncbi Nuchal edema and venous-lymphatic phenotype disturbance in human fetuses and mouse embryos with aneuploidy
    Mireille N Bekker
    Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, The Netherlands
    J Soc Gynecol Investig 13:209-16. 2006
    ..We hypothesize that a disturbed lymphatic endothelial differentiation and sac formation causes NE. We investigated endothelial differentiation of the jugular lymphatic system in human and mouse species with NE...
  4. ncbi The role of shear stress on ET-1, KLF2, and NOS-3 expression in the developing cardiovascular system of chicken embryos in a venous ligation model
    Bianca C W Groenendijk
    Department of Medical Biochemistry, Academic Medical Center, Amsterdam, The Netherlands
    Physiology (Bethesda) 22:380-9. 2007
    ..A set of endothelial shear stress-responsive genes coding for endothelin-1 (ET-1), lung Krüppel-like factor (LKLF/KLF2), and endothelial nitric oxide synthase (eNOS/NOS-3) are active in development and are specifically addressed...
  5. doi Podoplanin deficient mice show a RhoA-related hypoplasia of the sinus venosus myocardium including the sinoatrial node
    Edris A F Mahtab
    Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands
    Dev Dyn 238:183-93. 2009
    ..Our results demonstrate an important role for podoplanin in development of sinus venosus myocardium...
  6. ncbi The neural crest is contiguous with the cardiac conduction system in the mouse embryo: a role in induction?
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, Wassenaarseweg 62, PO 9602, Leiden, The Netherlands
    Anat Embryol (Berl) 208:389-93. 2004
    ..The contiguous expression of both reporters is suggestive for a potential role of cardiac NCC in the induction of the final differentiation of the CCS...
  7. pmc The development of the heart and microcirculation: role of shear stress
    Robert E Poelmann
    Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands
    Med Biol Eng Comput 46:479-84. 2008
    ..Geometry of the vascular system heavily affects the flow and shear patterns, as is the case in the adult vasculature where atheroprone areas nicely coincide with the frequency of the primary cilium as shear stress sensor...
  8. doi Cardiac development
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    ScientificWorldJournal 8:855-8. 2008
  9. ncbi Apoptosis as an instrument in cardiovascular development
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Birth Defects Res C Embryo Today 75:305-13. 2005
    ..In general, high shear stress protects against apoptosis, thus preventing the onset of disease processes in the fully-grown vasculature, and regulating the remodeling of the vascular system in the embryo...
  10. ncbi Developmental coronary maturation is disturbed by aberrant cardiac vascular endothelial growth factor expression and Notch signalling
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, PO Box 9600, 2300 RC Leiden, The Netherlands
    Cardiovasc Res 78:366-75. 2008
    ..Knowledge regarding the role of the VEGF/Notch/ephrinB2 cascade in (ab)normal coronary development will provide information on the subtle balance of VEGF signalling in coronary maturation and might enhance our therapeutic possibilities...
  11. ncbi Coronary artery and orifice development is associated with proper timing of epicardial outgrowth and correlated Fas-ligand-associated apoptosis patterns
    Ismail Eralp
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 96:526-34. 2005
    ..Also apoptosis in the peritruncal region is diminished. During later development, this leads to defective or absent connections of the coronary system to the systemic circulation...
  12. doi Accessory atrioventricular myocardial pathways in mouse heart development: substrate for supraventricular tachycardias
    Nathan D Hahurij
    Department of Pediatric Cardiology, Leiden University Medical Center, Leiden, 2300 RC, The Netherlands
    Pediatr Res 70:37-43. 2011
    ..We conclude that functional APs are present during normal mouse heart development. These APs can serve as transient substrate for AVRTs in the perinatal period of development...
  13. doi PDGF-B signaling is important for murine cardiac development: its role in developing atrioventricular valves, coronaries, and cardiac innervation
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 237:494-503. 2008
    ..This implies a role for PDGF-B/PDGFR-beta-signaling specifically in the contribution of these cell lineages to cardiac development...
  14. ncbi Transforming growth factor beta-SMAD2 signaling regulates aortic arch innervation and development
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9602, 2300 RC Leiden, The Netherlands
    Circ Res 95:1109-17. 2004
    ..We hypothesize that disturbed maturation of the fourth pharyngeal arch artery, and especially abrogated vascular innervation, will result in fourth arch interruptions...
  15. doi Morphogenesis of outflow tract rotation during cardiac development: the pulmonary push concept
    Roderick W C Scherptong
    Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 241:1413-22. 2012
    ..In wild type embryos (E9.5-14.5), second heart field contribution (SHF) to the OFT was studied using expression patterns of Islet 1, Nkx2.5, MLC-2a, WT-1, and 3D-reconstructions. Abnormal remodeling was studied in VEGF120/120 embryos...
  16. doi Cardiac malformations and myocardial abnormalities in podoplanin knockout mouse embryos: Correlation with abnormal epicardial development
    Edris A F Mahtab
    Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands
    Dev Dyn 237:847-57. 2008
    ..Abnormal epicardial differentiation and reduced epithelial-mesenchymal transformation result in deficient epicardium-derived cells leading to myocardial pathology and cardiac anomalies...
  17. pmc Epicardium-derived cells are important for correct development of the Purkinje fibers in the avian heart
    Ismail Eralp
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 288:1272-80. 2006
    ..We conclude that EPDCs are instrumental in Purkinje fiber differentiation, and we hypothesize that they cooperate directly with endothelial and endocardial cells in the development of the peripheral conduction system...
  18. doi Epicardium-derived cells in development of annulus fibrosis and persistence of accessory pathways
    Denise P Kolditz
    Department of Cardiology, Leiden University Medical Center, Albinusdreef 2, PO Box 9600, 2300 RC, Leiden, The Netherlands
    Circulation 117:1508-17. 2008
    ..In the present study, we investigated the role of epicardium-derived cells (EPDCs) in annulus fibrosis formation and the occurrence of APs...
  19. ncbi Spatiotemporally separated cardiac neural crest subpopulations that target the outflow tract septum and pharyngeal arch arteries
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 275:1009-18. 2003
    ..Our findings on the exact timing and migratory behavior of cardiac neural crest cells will help narrow the range of factors and genes that are involved in neural crest-related congenital heart diseases...
  20. ncbi Tetralogy of fallot and alterations in vascular endothelial growth factor-A signaling and notch signaling in mouse embryos solely expressing the VEGF120 isoform
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 100:842-9. 2007
    ..Defective right ventricular outflow tract development as presented provides new insight in the etiology of tetralogy of Fallot...
  21. ncbi Nkx2.5-negative myocardium of the posterior heart field and its correlation with podoplanin expression in cells from the developing cardiac pacemaking and conduction system
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec (Hoboken) 290:115-22. 2007
    ..We conclude that we have found podoplanin as a marker that links a novel Nkx2.5-negative sinus venosus myocardial area, which we refer to as the posterior heart field, with the cardiac conduction system...
  22. doi Tgfβ/Alk5 signaling is required for shear stress induced klf2 expression in embryonic endothelial cells
    Anastasia D Egorova
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 240:1670-80. 2011
    ..Results show that, in embryonic EC, shear stress activates Tgfβ/Alk5 signaling and that induction of Klf2 is an Alk5 dependent process...
  23. doi Platelet-derived growth factor is involved in the differentiation of second heart field-derived cardiac structures in chicken embryos
    Noortje A M Bax
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 238:2658-69. 2009
    ..Mechanical inhibition of epicardial outgrowth as well as chemical disturbance of PDGFR-alpha support a functional role of the ligands and the receptor in cardiac development...
  24. ncbi Embryonic conduction tissue: a spatial correlation with adult arrhythmogenic areas
    Monique R M Jongbloed
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    J Cardiovasc Electrophysiol 15:349-55. 2004
    ..To examine this hypothesis, we performed a detailed analysis of the developing cardiac conduction system using the recently described CCS-lacZ transgenic mouse strain...
  25. ncbi Platelet-derived growth factors in the developing avian heart and maturating coronary vasculature
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 233:1579-88. 2005
    ..Epicardium-derived cells in the atrioventricular cushions expressed PDGFR-beta. We conclude that all four proteins are involved in myocardial development, whereas PDGF-B and PDGFR-beta are specifically important in coronary maturation...
  26. doi Primary cilia sensitize endothelial cells for fluid shear stress
    Beerend P Hierck
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 237:725-35. 2008
    ..In addition, we targeted and stabilized the connection of the cilium to the cytoplasm by treatment with Colchicine and Taxol/Paclitaxel, respectively, and show that microtubular integrity is essential to sense shear stress...
  27. ncbi Development-related changes in the expression of shear stress responsive genes KLF-2, ET-1, and NOS-3 in the developing cardiovascular system of chicken embryos
    Bianca C W Groenendijk
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 230:57-68. 2004
    ..Our results suggest that, in the embryonic cardiovascular system, KLF-2 is expressed in regions of highest shear stress, and that ET-1 and NOS-3 expression, at least in the later stages, is related to shear stress...
  28. ncbi Intrauterine exposure to maternal atherosclerotic risk factors increases the susceptibility to atherosclerosis in adult life
    Fanneke E Alkemade
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Arterioscler Thromb Vasc Biol 27:2228-35. 2007
    ..We aimed to determine whether an in utero environment exposing a fetus to maternal hypercholesterolemia and associated risk factors can prime the murine vessel wall to accelerated development of cardiovascular disease in adult life...
  29. ncbi Changes in shear stress-related gene expression after experimentally altered venous return in the chicken embryo
    Bianca C W Groenendijk
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 96:1291-8. 2005
    ..We conclude that venous obstruction results in altered expression patterns of KLF2, ET-1, and NOS-3, suggestive for increased cardiac shear stress...
  30. doi The arterial and cardiac epicardium in development, disease and repair
    Adriana C Gittenberger-de Groot
    Department of Cardiology, Leiden University Medical Center, Postal zone S 5 24, P O Box 9600, 2300 RC Leiden, The Netherlands
    Differentiation 84:41-53. 2012
    ..Research into the many cell-autonomous and cell-cell-based capacities of the adult epicardium will open up new realistic therapeutic avenues...
  31. doi Funny current channel HCN4 delineates the developing cardiac conduction system in chicken heart
    Rebecca Vicente-Steijn
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Heart Rhythm 8:1254-63. 2011
    ..In the sinoatrial node (SAN), HCN4 is the predominant isoform responsible for the funny current. To date, no data are available on HCN4 expression during chicken CCS development...
  32. ncbi Cardiac outflow tract malformations in chick embryos exposed to homocysteine
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9602, 2300 RC Leiden, The Netherlands
    Cardiovasc Res 64:365-73. 2004
    ..The aim of this study is to investigate the effects of homocysteine on cardiac neural crest cells in relation to heart malformations...
  33. ncbi Origin, fate, and function of epicardium-derived cells (EPDCs) in normal and abnormal cardiac development
    Heleen Lie-Venema
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    ScientificWorldJournal 7:1777-98. 2007
    ..Lastly, we focus on the congenital heart anomalies that might be caused primarily by an epicardial developmental defect...
  34. ncbi Homocysteine induces endothelial cell detachment and vessel wall thickening during chick embryonic development
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 94:542-9. 2004
    ..We suggest that, as in adult life, increased homocysteine concentrations lead to vascular damage in the embryo. This prenatal damage might increase the susceptibility to develop vessel pathology later in life...
  35. doi Non-invasive tracking of avian development in vivo by MRI
    Bianca Hogers
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, P O Box 9600, 2300 RC Leiden, The Netherlands
    NMR Biomed 22:365-73. 2009
    ..In conclusion, micro-MRI can be used to evaluate in vivo early embryonic development and to diagnose cardiovascular malformations prenatally...
  36. doi A new direction for cardiac regeneration therapy: application of synergistically acting epicardium-derived cells and cardiomyocyte progenitor cells
    Elizabeth M Winter
    Departments of Anatomy and Embryology, Molecular Cell Biology, and Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Heart Fail 2:643-53. 2009
    ..Therefore, we transplanted EPDCs together with cardiomyocyte progenitor cells that can generate mature cardiomyocytes in vitro...
  37. ncbi Myocardial heterogeneity in permissiveness for epicardium-derived cells and endothelial precursor cells along the developing heart tube at the onset of coronary vascularization
    Heleen Lie-Venema
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 282:120-9. 2005
    ..We conclude that early coronary formation starts in the inner curvature and hypothesize that the presence of PEO-derived cells is essential for the maturation of the inner curvature and subsequent looping of the heart tube...
  38. doi Electrical activation of sinus venosus myocardium and expression patterns of RhoA and Isl-1 in the chick embryo
    Rebecca Vicente-Steijn
    Department of Anatomy and Embryology Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    J Cardiovasc Electrophysiol 21:1284-92. 2010
    ..Electrical Activity and RhoA in the Embryo...
  39. ncbi Epicardial cells of human adults can undergo an epithelial-to-mesenchymal transition and obtain characteristics of smooth muscle cells in vitro
    John van Tuyn
    Department of Cardiology, Leiden University Medical Center, Leiden, P O Box 9600, 2300 RC Leiden, The Netherlands
    Stem Cells 25:271-8. 2007
    ....
  40. doi TGF-β signaling in endothelial-to-mesenchymal transition: the role of shear stress and primary cilia
    Peter Ten Dijke
    Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, Netherlands
    Sci Signal 5:pt2. 2012
    ..Here, we show that shear stress activates TGF-β-Alk5 signaling in ECs, which is necessary for EndoMT in the cushions. Moreover, we show that the absence of a primary cilium is critically important for this transition process...
  41. ncbi Periostin expression by epicardium-derived cells is involved in the development of the atrioventricular valves and fibrous heart skeleton
    Heleen Lie-Venema
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden Postal zone S 1 P, P O Box 9600, 2300 RC Leiden, The Netherlands
    Differentiation 76:809-19. 2008
    ..The resulting cardiac anomalies might link to Wolff-Parkinson White syndrome with persistent AV myocardial connections...
  42. ncbi The endothelin-1 pathway and the development of cardiovascular defects in the haemodynamically challenged chicken embryo
    Bianca C W Groenendijk
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    J Vasc Res 45:54-68. 2008
    ..Therefore, we hypothesise that ET-1-related processes are involved in the development of functional and morphological cardiovascular defects after venous clip...
  43. ncbi Endothelial primary cilia in areas of disturbed flow are at the base of atherosclerosis
    Kim Van der Heiden
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Atherosclerosis 196:542-50. 2008
    ..Experimentally induced flow disturbance leads to induction of primary cilia, and subsequently to atherogenesis, which suggests a role for primary cilia in endothelial activation and dysfunction...
  44. ncbi Basics of cardiac development for the understanding of congenital heart malformations
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, P O Box 9602, 2300 RC Leiden, The Netherlands
    Pediatr Res 57:169-76. 2005
    ..Study of both (transgenic) animal models and human pathology is unravelling the mechanisms underlying congenital cardiac anomalies...
  45. ncbi Monocilia on chicken embryonic endocardium in low shear stress areas
    Kim Van der Heiden
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 235:19-28. 2006
    ..We demonstrate the presence of monocilia on endocardial-endothelial cells in areas of low shear stress and postulate that they are immotile primary cilia, which function as fluid shear stress sensors...
  46. pmc Self-gated CINE MRI for combined contrast-enhanced imaging and wall-stiffness measurements of murine aortic atherosclerotic lesions
    Brigit den Adel
    Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    PLoS ONE 8:e57299. 2013
    ....
  47. doi Endothelial colony-forming cells show a mature transcriptional response to shear stress
    Anastasia D Egorova
    Department of Anatomy and Embryology, Leiden University Medical Center, P O Box 9600, Postzone S 1 P, 2300 RC, Leiden, The Netherlands
    In Vitro Cell Dev Biol Anim 48:21-9. 2012
    ..This property supports the use of these cells as an EC source for tissue engineering applications...
  48. ncbi Connexin43 levels are increased in mouse neural crest cells exposed to homocysteine
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Birth Defects Res A Clin Mol Teratol 76:133-7. 2006
    ..Elevated homocysteine levels during embryonic development can result in neural tube and cardiovascular defects. The mechanisms that underlie the toxic effect of homocysteine are largely unknown...
  49. ncbi A chicken model for DGCR6 as a modifier gene in the DiGeorge critical region
    Beerend P Hierck
    Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Pediatr Res 56:440-8. 2004
    ..For the first time, DiGeorge syndrome is shown to be a contiguous gene syndrome in which not only several genes from the critical region, but also different cell types within the embryo, interact in the development of the phenotype...
  50. doi Accessory atrioventricular myocardial connections in the developing human heart: relevance for perinatal supraventricular tachycardias
    Nathan D Hahurij
    Department of Pediatric Cardiology, Leiden University Medical Center, Leiden, Netherlands
    Circulation 117:2850-8. 2008
    ..The transient presence of accessory AV myocardial connections during annulus fibrosus development may explain this phenomenon...
  51. ncbi Disturbed morphogenesis of cardiac outflow tract and increased rate of aortic arch anomalies in the offspring of diabetic rats
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Birth Defects Res A Clin Mol Teratol 70:927-38. 2004
    ..Hyperglycemia abrogates cardiovascular development in vitro; however, a link to cardiovascular defects in diabetic offspring remains to be investigated...
  52. ncbi The role of neural crest and epicardium-derived cells in conduction system formation
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9602, Leiden 2300 RC, The Netherlands
    Novartis Found Symp 250:125-34; discussion 134-41, 276-9. 2003
    ..For the Purkinje system there is a close approximation with the epicardium-derived cells. We postulate that both extracardiac-derived cell types have an influence on the differentiation of the definitive conduction system...
  53. doi Maternal transmission of risk for atherosclerosis
    Marco C DeRuiter
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Curr Opin Lipidol 19:333-7. 2008
    ..It is now generally accepted that epigenetic phenomena, such as either DNA methylation or chromatin modifications or both mediate the long-term memory and thus developmental programming of cells and tissues...
  54. pmc Echocardiographic assessment of embryonic and fetal mouse heart development: a focus on haemodynamics and morphology
    Nathan D Hahurij
    Department of Pediatric Cardiology, Leiden University Medical Center LUMC, Leiden, P O Box 9600, 2300 RC, Leiden, The Netherlands Department of Anatomy and Embryology, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    ScientificWorldJournal 2014:531324. 2014
    ..Conclusion. High-frequency echocardiography is a promising and useful imaging modality for structural and hemodynamic analysis of embryonic/fetal mouse hearts. ..
  55. doi Contrast enhancement by lipid-based MRI contrast agents in mouse atherosclerotic plaques; a longitudinal study
    Brigit den Adel
    Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands
    Contrast Media Mol Imaging 8:63-71. 2013
    ..This implies that, in the development of targeted contrast agents on a lipid-based backbone, extensive timing studies are required...
  56. ncbi Folic acid and homocysteine affect neural crest and neuroepithelial cell outgrowth and differentiation in vitro
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 227:301-8. 2003
    ..Our findings supports a critical role for folic acid and homocysteine in the development of neural tube defects and neural crest related heart malformations...
  57. pmc Lack of primary cilia primes shear-induced endothelial-to-mesenchymal transition
    Anastasia D Egorova
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 108:1093-101. 2011
    ..In the embryonic heart, high-flow regime applies to the endocardial cushion area, and the absence of cilia here coincides with the process of endothelial-to-mesenchymal transition (EndoMT)...
  58. doi Embryology of the heart and its impact on understanding fetal and neonatal heart disease
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands Electronic address
    Semin Fetal Neonatal Med 18:237-44. 2013
    ..This review focuses on several novel findings in cardiac development that might enhance our knowledge of aetiology and support refinement of prenatal diagnosis of congenital heart disease. ..
  59. doi Histological validation of iron-oxide and gadolinium based MRI contrast agents in experimental atherosclerosis: the do's and don't's
    Brigit den Adel
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Atherosclerosis 225:274-80. 2012
    ..The here presented techniques enable proper visualization of MR contrast agent accumulation and localization in atherosclerotic plaque, which will provide the validation necessary to advance these lipid-based nanoparticles to the clinic...
  60. doi Primary cilia as biomechanical sensors in regulating endothelial function
    Anastasia D Egorova
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Differentiation 83:S56-61. 2012
    ..Here we review the structural composition of the endothelial cilia and the current status of knowledge about the relation between the presence of primary cilia on endothelial cells and the shear stress to which they are exposed...
  61. doi Role for primary cilia as flow detectors in the cardiovascular system
    Kim Van der Heiden
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Int Rev Cell Mol Biol 290:87-119. 2011
    ..This review will cover their occurrence and elaborate on established and possible functions of primary cilia in the cardiovascular system...
  62. ncbi Changing intracellular compartmentalization of beta-galactosidase in the ROSA26 reporter mouse during embryonic development: a light- and electron-microscopic study
    Naoyoshi Aoyama
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 279:740-8. 2004
    ..Therefore, one must take the compartmental transition of intracellular beta-gal staining into consideration when interpreting results obtained from experiments using ROSA26 mice...
  63. ncbi Altered apoptosis pattern during pharyngeal arch artery remodelling is associated with aortic arch malformations in Tgfbeta2 knock-out mice
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Centre, P O Box 9602, 2300 RC, Leiden, The Netherlands
    Cardiovasc Res 56:312-22. 2002
    ..In order to unravel the underlying mechanism we studied the role of apoptosis in the normal regression of pharyngeal arch artery segments and in a mouse model that develops aortic arch malformations...
  64. pmc The role of the epicardium and neural crest as extracardiac contributors to coronary vascular development
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Tex Heart Inst J 29:255-61. 2002
    ....
  65. doi Fluid shear stress and inner curvature remodeling of the embryonic heart. Choosing the right lane!
    Beerend P Hierck
    1Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands
    ScientificWorldJournal 8:212-22. 2008
    ..The best of both worlds, mechanics and biology, are used here to describe early cardiogenesis...
  66. ncbi Collagen type VI expression during cardiac development and in human fetuses with trisomy 21
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 275:1109-16. 2003
    ..We hypothesise that collagen VI has a role in the development of heart defects involving endocardial cushion differentiation-specifically in the AV canal, the most common site of malformations affecting children with trisomy 21...
  67. ncbi Epicardium-derived cells (EPDCs) in development, cardiac disease and repair of ischemia
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    J Cell Mol Med 14:1056-60. 2010
    ..The finding of reactivation of the endogenous epicardium in ischemia with re-expression of developmental genes and renewed EMT marks the onset of a novel therapeutic focus...
  68. ncbi Conduction system abnormalities in rat embryos induced by maternal hyperthermia
    Naoyoshi Aoyama
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec 267:213-9. 2002
    ..Although hyperthermia causes marked hemodynamic defects, we could not obtain direct proof of a link between hemodynamic alteration by hyperthermia and malformations of the conduction system...
  69. ncbi T(1) relaxation in in vivo mouse brain at ultra-high field
    Rob C G van de Ven
    Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands
    Magn Reson Med 58:390-5. 2007
    ..4 T and 1824 +/- 101 to 2772 +/- 235 ms at 17.6 T. A field strength-dependent increase of T(1) relaxation times is shown. The SNR increase at 17.6 T is in good agreement with the expected SNR increase for a sample-dominated noise regime...
  70. ncbi Ventricular diastolic filling characteristics in stage-24 chick embryos after extra-embryonic venous obstruction
    Nicolette T C Ursem
    Department of Obstetrics and Gynecology, Erasmus MC, Rotterdam, 3000 DR, The Netherlands
    J Exp Biol 207:1487-90. 2004
    ..Ventricular filling components changed after partially obstructing the extra-embryonic venous circulation. These results suggest that material properties of the embryonic ventricle are modified after temporarily reduced hemodynamic load...
  71. doi Pitx2: a challenging teenager
    Robert E Poelmann
    Circ Res 102:749-51. 2008
  72. ncbi Systolic and diastolic ventricular function assessed by pressure-volume loops in the stage 21 venous clipped chick embryo
    Sandra Stekelenburg-de Vos
    Department of Obstetrics and Gynecology, Erasmus MC, 3015 GD Rotterdam, The Netherlands
    Pediatr Res 57:16-21. 2005
    ..Pressure-volume analysis may be applied in the chick embryo and is a sensitive technique to detect subtle alterations in ventricular function...
  73. ncbi Acutely altered hemodynamics following venous obstruction in the early chick embryo
    Sandra Stekelenburg-de Vos
    Department of Obstetrics and Gynaecology, Erasmus MC, Rotterdam, The Netherlands
    J Exp Biol 206:1051-7. 2003
    ..We conclude that the experimental alteration of venous return has major acute effects on hemodynamics in the chick embryo. These effects may be responsible for the observed cardiac malformations after clipping...
  74. doi Deciphering the endothelial shear stress sensor
    Robert E Poelmann
    Circulation 117:1124-6. 2008
  75. ncbi Evolution of an arsenal: structural and functional diversification of the venom system in the advanced snakes (Caenophidia)
    Bryan G Fry
    Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia
    Mol Cell Proteomics 7:215-46. 2008
    ....