Roman Koning

Summary

Affiliation: LUMC
Location: Leiden, Netherlands

Publications

  1. ncbi A 7.4-A projection structure of outer membrane phospholipase A from Escherichia coli by electron crystallography
    E J Boekema
    Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, NL 9747 AG, The Netherlands
    J Struct Biol 123:67-71. 1998
  2. pmc Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex
    Bert J C Janssen
    Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Utrecht, The Netherlands
    EMBO J 28:2469-78. 2009
  3. doi Shape and release control of a peptide decorated vesicle through pH sensitive orthogonal supramolecular interactions
    Frank Versluis
    Leiden Institute of Chemistry, University Leiden, P O Box 9502, 2300 RA, Leiden, The Netherlands
    J Am Chem Soc 131:13186-7. 2009
  4. doi Cryo-electron tomography of cellular microtubules
    Roman I Koning
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
    Methods Cell Biol 97:455-73. 2010
  5. doi A role for seipin in lipid droplet dynamics and inheritance in yeast
    Heimo Wolinski
    Institute of Molecular Biosciences, University of Graz, Humboldtstr 50 II, 8010 Graz, Austria
    J Cell Sci 124:3894-904. 2011
  6. doi Single-walled carbon nanotubes as scaffolds to concentrate DNA for the study of DNA-protein interactions
    Zunfeng Liu
    Gorlaeus Laboratory, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands
    Chemphyschem 13:1569-75. 2012
  7. doi Pushing the resolution limits in cryo electron tomography of biological structures
    Christoph A Diebolder
    Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    J Microsc 248:1-5. 2012
  8. doi Cellular nanoimaging by cryo electron tomography
    Roman I Koning
    Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
    Methods Mol Biol 950:227-51. 2013
  9. doi Cryo-electron tomography in biology and medicine
    Roman I Koning
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    Ann Anat 191:427-45. 2009
  10. doi Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15
    Linhua Jiang
    Department of Biophysical Structural Chemistry, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands
    J Mol Biol 386:1357-67. 2009

Collaborators

Detail Information

Publications25

  1. ncbi A 7.4-A projection structure of outer membrane phospholipase A from Escherichia coli by electron crystallography
    E J Boekema
    Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, NL 9747 AG, The Netherlands
    J Struct Biol 123:67-71. 1998
    ..A 7.4-A projection map reveals OMPLA molecules exhibiting an oval-shaped domain of 30 x 20 A resembling the beta-barrel structure characteristic of porins, which is associated with a 25-A elongated domain of lower density...
  2. pmc Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex
    Bert J C Janssen
    Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Utrecht, The Netherlands
    EMBO J 28:2469-78. 2009
    ..These insights into formation of convertases provide a basis for further development of complement therapeutics...
  3. doi Shape and release control of a peptide decorated vesicle through pH sensitive orthogonal supramolecular interactions
    Frank Versluis
    Leiden Institute of Chemistry, University Leiden, P O Box 9502, 2300 RA, Leiden, The Netherlands
    J Am Chem Soc 131:13186-7. 2009
    ..4 to 5.0, the formation of peptide beta-sheets on the vesicle surface induces a transition of the bilayer from a sphere to a fiber. This transition is fully reversible and repeatable. The vesicles release their cargo upon fiber formation...
  4. doi Cryo-electron tomography of cellular microtubules
    Roman I Koning
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
    Methods Cell Biol 97:455-73. 2010
    ....
  5. doi A role for seipin in lipid droplet dynamics and inheritance in yeast
    Heimo Wolinski
    Institute of Molecular Biosciences, University of Graz, Humboldtstr 50 II, 8010 Graz, Austria
    J Cell Sci 124:3894-904. 2011
    ....
  6. doi Single-walled carbon nanotubes as scaffolds to concentrate DNA for the study of DNA-protein interactions
    Zunfeng Liu
    Gorlaeus Laboratory, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands
    Chemphyschem 13:1569-75. 2012
    ..This provides an in vitro model for studying DNA-DNA and DNA-protein interactions at a high DNA concentration...
  7. doi Pushing the resolution limits in cryo electron tomography of biological structures
    Christoph A Diebolder
    Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    J Microsc 248:1-5. 2012
    ..Also, existing resolution measurement approaches and current technological developments that potentially increase the resolution in cryo electron tomography are discussed...
  8. doi Cellular nanoimaging by cryo electron tomography
    Roman I Koning
    Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
    Methods Mol Biol 950:227-51. 2013
    ....
  9. doi Cryo-electron tomography in biology and medicine
    Roman I Koning
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    Ann Anat 191:427-45. 2009
    ....
  10. doi Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15
    Linhua Jiang
    Department of Biophysical Structural Chemistry, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands
    J Mol Biol 386:1357-67. 2009
    ....
  11. ncbi Cryo electron tomography of vitrified fibroblasts: microtubule plus ends in situ
    Roman I Koning
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    J Struct Biol 161:459-68. 2008
    ..This suggests that frayed ends might be stabilized in the cell cortex...
  12. ncbi Schizosaccharomyces pombe Rad22A and Rad22B have similar biochemical properties and form multimeric structures
    Femke A T de Vries
    Department of Toxicogenetics, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Mutat Res 615:143-52. 2007
    ..Gel-filtration experiments and electron microscopic analyses indicate self-association of Rad22A and Rad22B and the formation of multimeric structures as has been observed for Rad52 in yeast and man...
  13. ncbi Cryo electron microscopy reconstructions of the Leviviridae unveil the densest icosahedral RNA packing possible
    Sjoerd H E van den Worm
    Department of Biochemistry, Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, 2300 RA, Leiden, The Netherlands
    J Mol Biol 363:858-65. 2006
    ..Strikingly, the unique RNA packing of the Leviviridae appears to deploy the most efficient method of RNA storage by obeying icosahedral symmetry...
  14. ncbi Mechanism of formation of multilayered 2D crystals of the enzyme IIC-mannitol transporter
    Marc C A Stuart
    Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Biochim Biophys Acta 1663:108-16. 2004
    ..This mechanism is inextricably associated with the formation of multilayers...
  15. ncbi The 5 A projection structure of the transmembrane domain of the mannitol transporter enzyme II
    R I Koning
    Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    J Mol Biol 287:845-51. 1999
    ..Each monomer exhibits six domains of high density which most likely correspond to transmembrane alpha-helices and cytoplasmic loops...
  16. ncbi Preparation of flat carbon support films
    Roman I Koning
    Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Nijenborgh 4, 9747, AG, The Netherlands
    Ultramicroscopy 94:183-91. 2003
    ..The formation of sandwiched carbon films was facilitated by the use of grids with thin bars. The carbon sandwich films were flat, stable, and easily and reproducibly prepared...
  17. ncbi TYSON: robust searching, sorting, and selecting of single particles in electron micrographs
    J R Plaisier
    Gorlaeus Laboratories, Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands
    J Struct Biol 145:76-83. 2004
    ..The procedures are fast and use of TYSON allowed, for example, some 20,000 particles to be selected in a single working day...
  18. ncbi Structure of the E. coli signal recognition particle bound to a translating ribosome
    Christiane Schaffitzel
    ETH Zurich, Institute for Molecular Biology and Biophysics, HPK Building, Schafmattstrasse 20, 8093 Zurich, Switzerland
    Nature 444:503-6. 2006
    ....
  19. doi Tools for correlative cryo-fluorescence microscopy and cryo-electron tomography applied to whole mitochondria in human endothelial cells
    Linda F van Driel
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands
    Eur J Cell Biol 88:669-84. 2009
    ..Taken together, this study emphasizes that cryo-CLEM can be a routinely used technique that opens up exciting new possibilities for cryo-ET...
  20. doi CsuA/BABCDE-dependent pili are not involved in the adherence of Acinetobacter baumannii ATCC19606(T) to human airway epithelial cells and their inflammatory response
    Anna de Breij
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Res Microbiol 160:213-8. 2009
    ..baumannii ATCC19606(T) to bronchial epithelial cells. The structure of the short pili and their possible role in adherence to human cells requires further investigation...
  21. ncbi MAVIS: An integrated system for live microscopy and vitrification
    Roman I Koning
    Department of Molecular Cell Biology, Albinusdreef 2, P O Box 9600, 2300 RC, Leiden University Medical Center, Leiden, The Netherlands Electronic address
    Ultramicroscopy 143:67-76. 2014
    ..Here we describe the design and performance of the MAVIS, illustrated with biological examples. ..
  22. doi Nanofabrication of a gold fiducial array on specimen support for electron tomography
    Roman I Koning
    Leiden University Medical Center, Department of Molecular Cell Biology, Section Electron Microscopy, P O Box 9600, 2300 RC Leiden, The Netherlands Electronic address
    Ultramicroscopy 135:99-104. 2013
    ..We showed that the support is suitable for electron tomography on plastic sections...
  23. doi Cryo-electron tomography analysis of membrane vesicles from Acinetobacter baumannii ATCC19606 T
    Roman I Koning
    Department of Molecular Cell Biology, Section Electron Microscopy, Einthovenweg 20, P O Box 9600, 2300 RC, Leiden University Medical Center, The Netherlands
    Res Microbiol 164:397-405. 2013
    ..Together, our results revealed multiple ways of vesicle formation leading to morphologically different MVs in the various stages of in vitro bacterial cultures...
  24. ncbi Visualization by cryo-electron microscopy of genomic RNA that binds to the protein capsid inside bacteriophage MS2
    Roman Koning
    Department of Molecular Cell Biology, Leiden University Medical Hospital, Wassenaarseweg 72, 2300 RA Leiden, The Netherlands
    J Mol Biol 332:415-22. 2003
    ..This RNA network is bound to the inside of the MS2 capsid and probably influences both capsid stability and formation, supporting the idea that capsid formation and RNA packaging are intimately linked to each other...