Adriana C Gittenberger-de Groot

Summary

Affiliation: Leiden University Medical Center
Country: The Netherlands

Publications

  1. ncbi request reprint Temporal and distinct TGFbeta ligand requirements during mouse and avian endocardial cushion morphogenesis
    Todd D Camenisch
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Scottsdale, AZ 85259, USA
    Dev Biol 248:170-81. 2002
  2. ncbi request reprint Development-related changes in the expression of shear stress responsive genes KLF-2, ET-1, and NOS-3 in the developing cardiovascular system of chicken embryos
    Bianca C W Groenendijk
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 230:57-68. 2004
  3. ncbi request reprint Transforming growth factor beta-SMAD2 signaling regulates aortic arch innervation and development
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9602, 2300 RC Leiden, The Netherlands
    Circ Res 95:1109-17. 2004
  4. doi request reprint PDGF-B signaling is important for murine cardiac development: its role in developing atrioventricular valves, coronaries, and cardiac innervation
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 237:494-503. 2008
  5. doi request reprint The lim domain only protein 7 is important in zebrafish heart development
    Elisabeth B Ott
    Institute of Biology, Department of Molecular and Cellular Biology, University of Leiden, Leiden, The Netherlands
    Dev Dyn 237:3940-52. 2008
  6. pmc Specific local cardiovascular changes of Nepsilon-(carboxymethyl)lysine, vascular endothelial growth factor, and Smad2 in the developing embryos coincide with maternal diabetes-induced congenital heart defects
    Pauline A M Roest
    Department of Anatomy and Embryology, Leiden University Medical Centre, Leiden, The Netherlands
    Diabetes 58:1222-8. 2009
  7. doi request reprint The arterial and cardiac epicardium in development, disease and repair
    Adriana C Gittenberger-de Groot
    Department of Cardiology, Leiden University Medical Center, Postal zone S 5 24, P O Box 9600, 2300 RC Leiden, The Netherlands
    Differentiation 84:41-53. 2012
  8. pmc Epicardium-derived cells in cardiogenesis and cardiac regeneration
    E M Winter
    Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2300 RC Leiden, The Netherlands
    Cell Mol Life Sci 64:692-703. 2007
  9. ncbi request reprint Epicardial outgrowth inhibition leads to compensatory mesothelial outflow tract collar and abnormal cardiac septation and coronary formation
    A C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 87:969-71. 2000
  10. ncbi request reprint Mannheimer Lecture. The quintessence of the making of the heart
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Cardiol Young 13:175-83. 2003

Detail Information

Publications95

  1. ncbi request reprint Temporal and distinct TGFbeta ligand requirements during mouse and avian endocardial cushion morphogenesis
    Todd D Camenisch
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Scottsdale, AZ 85259, USA
    Dev Biol 248:170-81. 2002
    ..Thus, both TGFbeta2 and TGFbeta3 appear necessary for the full morphogenetic program of EMT in the chick, but only TGFbeta2 is expressed and obligatory for mammalian endocardial cushion cell transformation...
  2. ncbi request reprint Development-related changes in the expression of shear stress responsive genes KLF-2, ET-1, and NOS-3 in the developing cardiovascular system of chicken embryos
    Bianca C W Groenendijk
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 230:57-68. 2004
    ..Our results suggest that, in the embryonic cardiovascular system, KLF-2 is expressed in regions of highest shear stress, and that ET-1 and NOS-3 expression, at least in the later stages, is related to shear stress...
  3. ncbi request reprint Transforming growth factor beta-SMAD2 signaling regulates aortic arch innervation and development
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9602, 2300 RC Leiden, The Netherlands
    Circ Res 95:1109-17. 2004
    ..We hypothesize that disturbed maturation of the fourth pharyngeal arch artery, and especially abrogated vascular innervation, will result in fourth arch interruptions...
  4. doi request reprint PDGF-B signaling is important for murine cardiac development: its role in developing atrioventricular valves, coronaries, and cardiac innervation
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 237:494-503. 2008
    ..This implies a role for PDGF-B/PDGFR-beta-signaling specifically in the contribution of these cell lineages to cardiac development...
  5. doi request reprint The lim domain only protein 7 is important in zebrafish heart development
    Elisabeth B Ott
    Institute of Biology, Department of Molecular and Cellular Biology, University of Leiden, Leiden, The Netherlands
    Dev Dyn 237:3940-52. 2008
    ..We reveal the temporal and spatial expression patterns of this important gene during mouse, chicken and fish development and our findings suggest essential functions for LMO7 during vertebrate heart development...
  6. pmc Specific local cardiovascular changes of Nepsilon-(carboxymethyl)lysine, vascular endothelial growth factor, and Smad2 in the developing embryos coincide with maternal diabetes-induced congenital heart defects
    Pauline A M Roest
    Department of Anatomy and Embryology, Leiden University Medical Centre, Leiden, The Netherlands
    Diabetes 58:1222-8. 2009
    ....
  7. doi request reprint The arterial and cardiac epicardium in development, disease and repair
    Adriana C Gittenberger-de Groot
    Department of Cardiology, Leiden University Medical Center, Postal zone S 5 24, P O Box 9600, 2300 RC Leiden, The Netherlands
    Differentiation 84:41-53. 2012
    ..Research into the many cell-autonomous and cell-cell-based capacities of the adult epicardium will open up new realistic therapeutic avenues...
  8. pmc Epicardium-derived cells in cardiogenesis and cardiac regeneration
    E M Winter
    Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2300 RC Leiden, The Netherlands
    Cell Mol Life Sci 64:692-703. 2007
    ..These cells can therefore be considered as a novel source of adult stem cells useful in clinical cardiac regeneration therapy...
  9. ncbi request reprint Epicardial outgrowth inhibition leads to compensatory mesothelial outflow tract collar and abnormal cardiac septation and coronary formation
    A C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 87:969-71. 2000
    ..Heterospecific epicardial transplant is able to partially rescue the myocardial development, as well as septation and coronary formation...
  10. ncbi request reprint Mannheimer Lecture. The quintessence of the making of the heart
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Cardiol Young 13:175-83. 2003
    ..I conclude by discussing specific malformations of the heart that might be linked to these extracardiac contributions...
  11. ncbi request reprint Basics of cardiac development for the understanding of congenital heart malformations
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, P O Box 9602, 2300 RC Leiden, The Netherlands
    Pediatr Res 57:169-76. 2005
    ..Study of both (transgenic) animal models and human pathology is unravelling the mechanisms underlying congenital cardiac anomalies...
  12. ncbi request reprint Nkx2.5-negative myocardium of the posterior heart field and its correlation with podoplanin expression in cells from the developing cardiac pacemaking and conduction system
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec (Hoboken) 290:115-22. 2007
    ..We conclude that we have found podoplanin as a marker that links a novel Nkx2.5-negative sinus venosus myocardial area, which we refer to as the posterior heart field, with the cardiac conduction system...
  13. ncbi request reprint Collagen type VI expression during cardiac development and in human fetuses with trisomy 21
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 275:1109-16. 2003
    ..We hypothesise that collagen VI has a role in the development of heart defects involving endocardial cushion differentiation-specifically in the AV canal, the most common site of malformations affecting children with trisomy 21...
  14. ncbi request reprint Transforming growth factor beta-SMAD2 signaling and aortic arch development
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Trends Cardiovasc Med 16:1-6. 2006
    ..A relation between apoptosis and flow is postulated, in which a role for genes with a shear stress-responsive element, including the endothelially expressed transforming growth factor beta1, is implied...
  15. ncbi request reprint Development of the coronary vasculature and its implications for coronary abnormalities in general and specifically in pulmonary atresia without ventricular septal defect
    A C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Centre, Leiden, The Netherlands
    Acta Paediatr Suppl 93:13-9. 2004
    ..Coronary vascular anomalies are an important factor in congenital heart disease in the neonate. However, our knowledge of the pathomorphogenesis is still defective...
  16. ncbi request reprint Abnormal lymphatic development in trisomy 16 mouse embryos precedes nuchal edema
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 230:378-84. 2004
    ..A disturbance of lymphangiogenesis might be the basis for increased nuchal translucency that is often observed in diseased human fetuses...
  17. doi request reprint Epicardium-derived cells (EPDCs) in development, cardiac disease and repair of ischemia
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    J Cell Mol Med 14:1056-60. 2010
    ..The finding of reactivation of the endogenous epicardium in ischemia with re-expression of developmental genes and renewed EMT marks the onset of a novel therapeutic focus...
  18. ncbi request reprint Tetralogy of fallot and alterations in vascular endothelial growth factor-A signaling and notch signaling in mouse embryos solely expressing the VEGF120 isoform
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 100:842-9. 2007
    ..Defective right ventricular outflow tract development as presented provides new insight in the etiology of tetralogy of Fallot...
  19. ncbi request reprint Coronary artery and orifice development is associated with proper timing of epicardial outgrowth and correlated Fas-ligand-associated apoptosis patterns
    Ismail Eralp
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 96:526-34. 2005
    ..Also apoptosis in the peritruncal region is diminished. During later development, this leads to defective or absent connections of the coronary system to the systemic circulation...
  20. doi request reprint Cardiac malformations and myocardial abnormalities in podoplanin knockout mouse embryos: Correlation with abnormal epicardial development
    Edris A F Mahtab
    Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands
    Dev Dyn 237:847-57. 2008
    ..Abnormal epicardial differentiation and reduced epithelial-mesenchymal transformation result in deficient epicardium-derived cells leading to myocardial pathology and cardiac anomalies...
  21. doi request reprint A new direction for cardiac regeneration therapy: application of synergistically acting epicardium-derived cells and cardiomyocyte progenitor cells
    Elizabeth M Winter
    Departments of Anatomy and Embryology, Molecular Cell Biology, and Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Heart Fail 2:643-53. 2009
    ..Therefore, we transplanted EPDCs together with cardiomyocyte progenitor cells that can generate mature cardiomyocytes in vitro...
  22. doi request reprint Accessory atrioventricular myocardial pathways in mouse heart development: substrate for supraventricular tachycardias
    Nathan D Hahurij
    Department of Pediatric Cardiology, Leiden University Medical Center, Leiden, 2300 RC, The Netherlands
    Pediatr Res 70:37-43. 2011
    ..We conclude that functional APs are present during normal mouse heart development. These APs can serve as transient substrate for AVRTs in the perinatal period of development...
  23. ncbi request reprint Mesenchymal stem cells from ischemic heart disease patients improve left ventricular function after acute myocardial infarction
    Robert W Grauss
    Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    Am J Physiol Heart Circ Physiol 293:H2438-47. 2007
    ..hMSCs from patients with IHD engraft in infarcted mouse myocardium and preserve LV function 2 wk after AMI, potentially through an enhancement of scar vascularity and a reduction of wall thinning...
  24. ncbi request reprint Epicardial cells of human adults can undergo an epithelial-to-mesenchymal transition and obtain characteristics of smooth muscle cells in vitro
    John van Tuyn
    Department of Cardiology, Leiden University Medical Center, Leiden, P O Box 9600, 2300 RC Leiden, The Netherlands
    Stem Cells 25:271-8. 2007
    ....
  25. doi request reprint Morphogenesis of outflow tract rotation during cardiac development: the pulmonary push concept
    Roderick W C Scherptong
    Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 241:1413-22. 2012
    ..In wild type embryos (E9.5-14.5), second heart field contribution (SHF) to the OFT was studied using expression patterns of Islet 1, Nkx2.5, MLC-2a, WT-1, and 3D-reconstructions. Abnormal remodeling was studied in VEGF120/120 embryos...
  26. doi request reprint Podoplanin deficient mice show a RhoA-related hypoplasia of the sinus venosus myocardium including the sinoatrial node
    Edris A F Mahtab
    Department of Anatomy and Embryology, Leiden University Medical Center, The Netherlands
    Dev Dyn 238:183-93. 2009
    ..Our results demonstrate an important role for podoplanin in development of sinus venosus myocardium...
  27. ncbi request reprint The neural crest is contiguous with the cardiac conduction system in the mouse embryo: a role in induction?
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, Wassenaarseweg 62, PO 9602, Leiden, The Netherlands
    Anat Embryol (Berl) 208:389-93. 2004
    ..The contiguous expression of both reporters is suggestive for a potential role of cardiac NCC in the induction of the final differentiation of the CCS...
  28. doi request reprint Epicardium-derived cells in development of annulus fibrosis and persistence of accessory pathways
    Denise P Kolditz
    Department of Cardiology, Leiden University Medical Center, Albinusdreef 2, PO Box 9600, 2300 RC, Leiden, The Netherlands
    Circulation 117:1508-17. 2008
    ..In the present study, we investigated the role of epicardium-derived cells (EPDCs) in annulus fibrosis formation and the occurrence of APs...
  29. doi request reprint Disturbed myocardial connexin 43 and N-cadherin expressions in hypoplastic left heart syndrome and borderline left ventricle
    Edris A F Mahtab
    Department of Anatomy and Embryology, Leiden University Medical Center LUMC, Leiden, The Netherlands
    J Thorac Cardiovasc Surg 144:1315-22. 2012
    ..Wondering whether it is possible to identify cases suitable for biventricular repair from a developmental viewpoint, we investigated the myocardial histology of borderline and severely hypoplastic left ventricles...
  30. ncbi request reprint Developmental coronary maturation is disturbed by aberrant cardiac vascular endothelial growth factor expression and Notch signalling
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, PO Box 9600, 2300 RC Leiden, The Netherlands
    Cardiovasc Res 78:366-75. 2008
    ..Knowledge regarding the role of the VEGF/Notch/ephrinB2 cascade in (ab)normal coronary development will provide information on the subtle balance of VEGF signalling in coronary maturation and might enhance our therapeutic possibilities...
  31. pmc Cardiac malformations in Pdgfralpha mutant embryos are associated with increased expression of WT1 and Nkx2.5 in the second heart field
    Noortje A M Bax
    Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Dev Dyn 239:2307-17. 2010
    ..Our data provide novel insight for a possible role for Pdgfralpha in transduction pathways that lead to repression of Nkx2.5 and WT1 during development of posterior heart field-derived cardiac structures...
  32. ncbi request reprint Cardiac outflow tract malformations in chick embryos exposed to homocysteine
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9602, 2300 RC Leiden, The Netherlands
    Cardiovasc Res 64:365-73. 2004
    ..The aim of this study is to investigate the effects of homocysteine on cardiac neural crest cells in relation to heart malformations...
  33. ncbi request reprint Intrauterine exposure to maternal atherosclerotic risk factors increases the susceptibility to atherosclerosis in adult life
    Fanneke E Alkemade
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Arterioscler Thromb Vasc Biol 27:2228-35. 2007
    ..We aimed to determine whether an in utero environment exposing a fetus to maternal hypercholesterolemia and associated risk factors can prime the murine vessel wall to accelerated development of cardiovascular disease in adult life...
  34. pmc Epicardium-derived cells are important for correct development of the Purkinje fibers in the avian heart
    Ismail Eralp
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 288:1272-80. 2006
    ..We conclude that EPDCs are instrumental in Purkinje fiber differentiation, and we hypothesize that they cooperate directly with endothelial and endocardial cells in the development of the peripheral conduction system...
  35. ncbi request reprint Platelet-derived growth factors in the developing avian heart and maturating coronary vasculature
    Nynke M S Van Den Akker
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 233:1579-88. 2005
    ..Epicardium-derived cells in the atrioventricular cushions expressed PDGFR-beta. We conclude that all four proteins are involved in myocardial development, whereas PDGF-B and PDGFR-beta are specifically important in coronary maturation...
  36. pmc Prenatal exposure to apoE deficiency and postnatal hypercholesterolemia are associated with altered cell-specific lysine methyltransferase and histone methylation patterns in the vasculature
    Fanneke E Alkemade
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Am J Pathol 176:542-8. 2010
    ..Our results suggest that both in utero programming and postnatal hypercholesterolemia affect epigenetic patterning in the vasculature, thereby providing novel insights regarding initiation and progression of vascular disease in adults...
  37. ncbi request reprint Spatiotemporally separated cardiac neural crest subpopulations that target the outflow tract septum and pharyngeal arch arteries
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 275:1009-18. 2003
    ..Our findings on the exact timing and migratory behavior of cardiac neural crest cells will help narrow the range of factors and genes that are involved in neural crest-related congenital heart diseases...
  38. ncbi request reprint Homocysteine induces endothelial cell detachment and vessel wall thickening during chick embryonic development
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 94:542-9. 2004
    ..We suggest that, as in adult life, increased homocysteine concentrations lead to vascular damage in the embryo. This prenatal damage might increase the susceptibility to develop vessel pathology later in life...
  39. doi request reprint Platelet-derived growth factor is involved in the differentiation of second heart field-derived cardiac structures in chicken embryos
    Noortje A M Bax
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 238:2658-69. 2009
    ..Mechanical inhibition of epicardial outgrowth as well as chemical disturbance of PDGFR-alpha support a functional role of the ligands and the receptor in cardiac development...
  40. ncbi request reprint Altered apoptosis pattern during pharyngeal arch artery remodelling is associated with aortic arch malformations in Tgfbeta2 knock-out mice
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Centre, P O Box 9602, 2300 RC, Leiden, The Netherlands
    Cardiovasc Res 56:312-22. 2002
    ..In order to unravel the underlying mechanism we studied the role of apoptosis in the normal regression of pharyngeal arch artery segments and in a mouse model that develops aortic arch malformations...
  41. doi request reprint Epithelial-to-mesenchymal transformation alters electrical conductivity of human epicardial cells
    Noortje A M Bax
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    J Cell Mol Med 15:2675-83. 2011
    ..This study shows for the first time the conduction differences between epicardial cells before and after EMT. These differences may be of relevance for the role of EPDCs in cardiac development, and in EMT-related cardiac dysfunction...
  42. ncbi request reprint Myocardial heterogeneity in permissiveness for epicardium-derived cells and endothelial precursor cells along the developing heart tube at the onset of coronary vascularization
    Heleen Lie-Venema
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 282:120-9. 2005
    ..We conclude that early coronary formation starts in the inner curvature and hypothesize that the presence of PEO-derived cells is essential for the maturation of the inner curvature and subsequent looping of the heart tube...
  43. ncbi request reprint The myth of ventrally emigrating neural tube (VENT) cells and their contribution to the developing cardiovascular system
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
    Anat Embryol (Berl) 206:327-33. 2003
    ..We conclude that there is no contribution of ventral neural tube cells to the developing cardiovascular system...
  44. ncbi request reprint Persistence of functional atrioventricular accessory pathways in postseptated embryonic avian hearts: implications for morphogenesis and functional maturation of the cardiac conduction system
    Denise P Kolditz
    Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    Circulation 115:17-26. 2007
    ..We investigated the possibility of impulse propagation through remnants of atrioventricular (AV) connections in quail hearts...
  45. doi request reprint Periostin expression by epicardium-derived cells is involved in the development of the atrioventricular valves and fibrous heart skeleton
    Heleen Lie-Venema
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden Postal zone S 1 P, P O Box 9600, 2300 RC Leiden, The Netherlands
    Differentiation 76:809-19. 2008
    ..The resulting cardiac anomalies might link to Wolff-Parkinson White syndrome with persistent AV myocardial connections...
  46. doi request reprint Electrical activation of sinus venosus myocardium and expression patterns of RhoA and Isl-1 in the chick embryo
    Rebecca Vicente-Steijn
    Department of Anatomy and Embryology Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    J Cardiovasc Electrophysiol 21:1284-92. 2010
    ..Electrical Activity and RhoA in the Embryo...
  47. doi request reprint Funny current channel HCN4 delineates the developing cardiac conduction system in chicken heart
    Rebecca Vicente-Steijn
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Heart Rhythm 8:1254-63. 2011
    ..In the sinoatrial node (SAN), HCN4 is the predominant isoform responsible for the funny current. To date, no data are available on HCN4 expression during chicken CCS development...
  48. ncbi request reprint Origin, fate, and function of epicardium-derived cells (EPDCs) in normal and abnormal cardiac development
    Heleen Lie-Venema
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    ScientificWorldJournal 7:1777-98. 2007
    ..Lastly, we focus on the congenital heart anomalies that might be caused primarily by an epicardial developmental defect...
  49. ncbi request reprint Embryonic conduction tissue: a spatial correlation with adult arrhythmogenic areas
    Monique R M Jongbloed
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    J Cardiovasc Electrophysiol 15:349-55. 2004
    ..To examine this hypothesis, we performed a detailed analysis of the developing cardiac conduction system using the recently described CCS-lacZ transgenic mouse strain...
  50. pmc The role of the epicardium and neural crest as extracardiac contributors to coronary vascular development
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Tex Heart Inst J 29:255-61. 2002
    ....
  51. doi request reprint Abnormal Shh and FOXC2 expression correlates with aberrant lymphatic development in human fetuses with increased nuchal translucency
    Yolanda M de Mooij
    Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Prenat Diagn 29:840-6. 2009
    ....
  52. ncbi request reprint Exposure of neural crest cells to elevated glucose leads to congenital heart defects, an effect that can be prevented by N-acetylcysteine
    Pauline A M Roest
    Department of Anatomy and Embryology, Leiden University Medical Centre, Leiden, The Netherlands
    Birth Defects Res A Clin Mol Teratol 79:231-5. 2007
    ..Antioxidants reduce the damaging effect of glucose on neural crest cells in vitro; therefore, we investigated the effect of supplementing N-acetylcysteine in vivo...
  53. doi request reprint Forced myocardin expression enhances the therapeutic effect of human mesenchymal stem cells after transplantation in ischemic mouse hearts
    Robert W Grauss
    Department of Cardiology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Stem Cells 26:1083-93. 2008
    ..Furthermore, forced myocardin expression improved engraftment and induced a cardiomyocyte-like phenotype hMSC differentiation...
  54. doi request reprint Cardiomyogenic differentiation-independent improvement of cardiac function by human cardiomyocyte progenitor cell injection in ischaemic mouse hearts
    Melina C den Haan
    Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    J Cell Mol Med 16:1508-21. 2012
    ..These results demonstrate that hCMPCs can be applied to repair infarcted myocardium without the need to undergo differentiation into cardiomyocytes...
  55. doi request reprint Accessory atrioventricular myocardial connections in the developing human heart: relevance for perinatal supraventricular tachycardias
    Nathan D Hahurij
    Department of Pediatric Cardiology, Leiden University Medical Center, Leiden, Netherlands
    Circulation 117:2850-8. 2008
    ..The transient presence of accessory AV myocardial connections during annulus fibrosus development may explain this phenomenon...
  56. ncbi request reprint Endothelial primary cilia in areas of disturbed flow are at the base of atherosclerosis
    Kim Van der Heiden
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Atherosclerosis 196:542-50. 2008
    ..Experimentally induced flow disturbance leads to induction of primary cilia, and subsequently to atherogenesis, which suggests a role for primary cilia in endothelial activation and dysfunction...
  57. ncbi request reprint Ets-1 and Ets-2 transcription factors are essential for normal coronary and myocardial development in chicken embryos
    Heleen Lie-Venema
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9602, 2300 RC Leiden, The Netherlands
    Circ Res 92:749-56. 2003
    ..The presence of coronary vascular aberrations in the antisense-Ets phenotype enabled us for the first time to study abnormal coronary development in a model that is not lethal to the embryo...
  58. doi request reprint Endothelial colony-forming cells show a mature transcriptional response to shear stress
    Anastasia D Egorova
    Department of Anatomy and Embryology, Leiden University Medical Center, P O Box 9600, Postzone S 1 P, 2300 RC, Leiden, The Netherlands
    In Vitro Cell Dev Biol Anim 48:21-9. 2012
    ..This property supports the use of these cells as an EC source for tissue engineering applications...
  59. ncbi request reprint A chicken model for DGCR6 as a modifier gene in the DiGeorge critical region
    Beerend P Hierck
    Department of Anatomy and Embryology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Pediatr Res 56:440-8. 2004
    ..For the first time, DiGeorge syndrome is shown to be a contiguous gene syndrome in which not only several genes from the critical region, but also different cell types within the embryo, interact in the development of the phenotype...
  60. doi request reprint Non-invasive tracking of avian development in vivo by MRI
    Bianca Hogers
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, P O Box 9600, 2300 RC Leiden, The Netherlands
    NMR Biomed 22:365-73. 2009
    ..In conclusion, micro-MRI can be used to evaluate in vivo early embryonic development and to diagnose cardiovascular malformations prenatally...
  61. doi request reprint Tgfβ/Alk5 signaling is required for shear stress induced klf2 expression in embryonic endothelial cells
    Anastasia D Egorova
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 240:1670-80. 2011
    ..Results show that, in embryonic EC, shear stress activates Tgfβ/Alk5 signaling and that induction of Klf2 is an Alk5 dependent process...
  62. pmc The development of the heart and microcirculation: role of shear stress
    Robert E Poelmann
    Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands
    Med Biol Eng Comput 46:479-84. 2008
    ..Geometry of the vascular system heavily affects the flow and shear patterns, as is the case in the adult vasculature where atheroprone areas nicely coincide with the frequency of the primary cilium as shear stress sensor...
  63. doi request reprint Aberrant lymphatic development in euploid fetuses with increased nuchal translucency including Noonan syndrome
    Yolanda M de Mooij
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Prenat Diagn 31:159-66. 2011
    ..In 60–70% of the Noonan syndrome cases, a gene mutation can be demonstrated. Previous research showed that aneuploid fetuses with increased nuchal translucency (NT) demonstrate an aberrant lymphatic endothelial differentiation...
  64. doi request reprint The outflow tract in transposition of the great arteries: an anatomic and morphologic study
    Shirin Lalezari
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Ann Thorac Surg 88:1300-5. 2009
    ....
  65. ncbi request reprint Changes in shear stress-related gene expression after experimentally altered venous return in the chicken embryo
    Bianca C W Groenendijk
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Circ Res 96:1291-8. 2005
    ..We conclude that venous obstruction results in altered expression patterns of KLF2, ET-1, and NOS-3, suggestive for increased cardiac shear stress...
  66. doi request reprint Maternal transmission of risk for atherosclerosis
    Marco C DeRuiter
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Curr Opin Lipidol 19:333-7. 2008
    ..It is now generally accepted that epigenetic phenomena, such as either DNA methylation or chromatin modifications or both mediate the long-term memory and thus developmental programming of cells and tissues...
  67. ncbi request reprint Expression patterns of Tgfbeta1-3 associate with myocardialisation of the outflow tract and the development of the epicardium and the fibrous heart skeleton
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 227:431-44. 2003
    ..The information provided favors novel roles of Tgfbeta1-3 in epicardial development, of Tgfbeta2 in myocardialisation, and of Tgfbeta3 in differentiation of the fibrous structures of the heart...
  68. pmc Differential temporal and spatial progerin expression during closure of the ductus arteriosus in neonates
    Regina Bokenkamp
    Department of Pediatric Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    PLoS ONE 6:e23975. 2011
    ..We suggest that activation of LMNA alternative splicing is involved in vascular remodeling in the circulatory system during normal neonatal DA closure...
  69. ncbi request reprint Changing intracellular compartmentalization of beta-galactosidase in the ROSA26 reporter mouse during embryonic development: a light- and electron-microscopic study
    Naoyoshi Aoyama
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec A Discov Mol Cell Evol Biol 279:740-8. 2004
    ..Therefore, one must take the compartmental transition of intracellular beta-gal staining into consideration when interpreting results obtained from experiments using ROSA26 mice...
  70. ncbi request reprint Monocilia on chicken embryonic endocardium in low shear stress areas
    Kim Van der Heiden
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 235:19-28. 2006
    ..We demonstrate the presence of monocilia on endocardial-endothelial cells in areas of low shear stress and postulate that they are immotile primary cilia, which function as fluid shear stress sensors...
  71. pmc Chronic right ventricular pressure overload results in a hyperplastic rather than a hypertrophic myocardial response
    Boudewijn P J Leeuwenburgh
    Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    J Anat 212:286-94. 2008
    ..Chronic right ventricular pressure overload applied in neonatal sheep hearts results in a significant increase in right ventricular free wall thickness which is primarily the result of a hyperplastic myocardial response...
  72. ncbi request reprint The role of neural crest and epicardium-derived cells in conduction system formation
    Adriana C Gittenberger-de Groot
    Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9602, Leiden 2300 RC, The Netherlands
    Novartis Found Symp 250:125-34; discussion 134-41, 276-9. 2003
    ..For the Purkinje system there is a close approximation with the epicardium-derived cells. We postulate that both extracardiac-derived cell types have an influence on the differentiation of the definitive conduction system...
  73. ncbi request reprint Folic acid and homocysteine affect neural crest and neuroepithelial cell outgrowth and differentiation in vitro
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Dev Dyn 227:301-8. 2003
    ..Our findings supports a critical role for folic acid and homocysteine in the development of neural tube defects and neural crest related heart malformations...
  74. ncbi request reprint Connexin43 levels are increased in mouse neural crest cells exposed to homocysteine
    Marit J Boot
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Birth Defects Res A Clin Mol Teratol 76:133-7. 2006
    ..Elevated homocysteine levels during embryonic development can result in neural tube and cardiovascular defects. The mechanisms that underlie the toxic effect of homocysteine are largely unknown...
  75. ncbi request reprint Conduction system abnormalities in rat embryos induced by maternal hyperthermia
    Naoyoshi Aoyama
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Anat Rec 267:213-9. 2002
    ..Although hyperthermia causes marked hemodynamic defects, we could not obtain direct proof of a link between hemodynamic alteration by hyperthermia and malformations of the conduction system...
  76. ncbi request reprint Development of the cardiac conduction system in atrioventricular septal defect in human trisomy 21
    Nico A Blom
    Department of Pediatric Cardiology, Leiden University Medical Center, The Netherlands
    Pediatr Res 58:516-20. 2005
    ..The formation of the AV node and the ventricular conduction system in AVSD and Down syndrome differs from normal development, which can be a causative factor in the development of AV conduction disturbances...
  77. ncbi request reprint Disturbed morphogenesis of cardiac outflow tract and increased rate of aortic arch anomalies in the offspring of diabetic rats
    Daniel G M Molin
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Birth Defects Res A Clin Mol Teratol 70:927-38. 2004
    ..Hyperglycemia abrogates cardiovascular development in vitro; however, a link to cardiovascular defects in diabetic offspring remains to be investigated...
  78. ncbi request reprint Histological evaluation of decellularised porcine aortic valves: matrix changes due to different decellularisation methods
    Robert W Grauss
    Department of Anatomy and Embryology, Leiden University Medical Center, P O Box 9602, 2300 RC Leiden, The Netherlands
    Eur J Cardiothorac Surg 27:566-71. 2005
    ..However, the effect of chemically induced decellularisation on the major structural and adhesion molecules as well as glycosaminoglycans, and the possible replenishment of lost compounds have escaped attention...
  79. ncbi request reprint Deficiency of the vestibular spine in atrioventricular septal defects in human fetuses with down syndrome
    Nico A Blom
    Department of Pediatric Cardiology, Leiden University Medical Center, Leiden, The Netherlands
    Am J Cardiol 91:180-4. 2003
    ..Therefore, reduced expansion of the vestibular spine derived from the dorsal mesocardium appears to play an important role in the development of AVSD in Down syndrome...
  80. ncbi request reprint Pathogenesis of congenital cardiac malformations and mechanisms of cardiac remodelling
    Arnold C G Wenink
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Cardiol Young 15:3-6. 2005
  81. ncbi request reprint The morphologic nature of noncommitted ventricular septal defects in specimens with double-outlet right ventricle
    Rudolf P Beekman
    Center for Congenital Heart Disease, Amsterdam and Leiden, The Netherlands
    J Thorac Cardiovasc Surg 124:984-90. 2002
    ..However, the term noncommitted ventricular septal defect remains enigmatic. The aim of this study was to elucidate the morphologic nature of the noncommitted ventricular septal defect in view of its surgical implications...
  82. ncbi request reprint Apoptosis as an instrument in cardiovascular development
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Birth Defects Res C Embryo Today 75:305-13. 2005
    ..In general, high shear stress protects against apoptosis, thus preventing the onset of disease processes in the fully-grown vasculature, and regulating the remodeling of the vascular system in the embryo...
  83. doi request reprint Cardiac development
    Robert E Poelmann
    Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    ScientificWorldJournal 8:855-8. 2008
  84. ncbi request reprint Increased nuchal translucency is associated with jugular lymphatic distension
    Monique C Haak
    Department of Obstetrics and Gynaecology, VU Medical Center, Amsterdam, The Netherlands
    Hum Reprod 17:1086-92. 2002
    ..Increased NT is seen in a diversity of fetal malformations. The mechanism explaining the abnormal fluid accumulation and the transient nature of NT remains unexplained...
  85. pmc Transforming growth factor beta in cardiovascular development and function
    Mohamad Azhar
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
    Cytokine Growth Factor Rev 14:391-407. 2003
    ..The evidence for TGFbeta activities during cardiovascular development and physiologic function will be given and areas which need further investigation will be discussed...
  86. pmc Transcription factor Sp3 knockout mice display serious cardiac malformations
    Pieter Fokko Van Loo
    Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands
    Mol Cell Biol 27:8571-82. 2007
    ..Wt1 expression was diminished in epicardium-derived cells in the myocardium of Sp3 null hearts. We conclude that Sp3 is required for normal cardiac development and suggest that it has a crucial role in myocardial differentiation...
  87. ncbi request reprint Left atrial tachycardia originating from the mitral annulus-aorta junction
    Mario D Gonzalez
    Division of Cardiovascular Medicine, University of Florida College of Medicine, PO Box 100277, Gainesville, FL 32610 0277, USA
    Circulation 110:3187-92. 2004
    ..Under experimental conditions, this region can generate abnormal electrical activity. In patients with left atrial tachycardia, we investigated whether this region could be the source of this arrhythmia...
  88. ncbi request reprint Targeted mutation reveals essential functions of the homeodomain transcription factor Shox2 in sinoatrial and pacemaking development
    Rudiger J Blaschke
    Human Genetics, University of Heidelberg, Heidelberg, Germany
    Circulation 115:1830-8. 2007
    ..Here, we report on a highly restricted expression pattern of the homeodomain transcription factor Shox2 in the sinus venosus myocardium, including the sinoatrial nodal region and the venous valves...
  89. doi request reprint Pitx2: a challenging teenager
    Robert E Poelmann
    Circ Res 102:749-51. 2008
  90. doi request reprint Jugular lymphatic maldevelopment in Turner syndrome and trisomy 21: different anomalies leading to nuchal edema
    Mireile N Bekker
    Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, The Netherlands
    Reprod Sci 15:295-304. 2008
    ..In Turner fetuses, no jugular lymphatic sac was observed. Nuchal edema in trisomy 21 and Turner syndrome appears to be a similar entity caused by different lymphatic abnormalities...
  91. ncbi request reprint Ventricular diastolic filling characteristics in stage-24 chick embryos after extra-embryonic venous obstruction
    Nicolette T C Ursem
    Department of Obstetrics and Gynecology, Erasmus MC, Rotterdam, 3000 DR, The Netherlands
    J Exp Biol 207:1487-90. 2004
    ..Ventricular filling components changed after partially obstructing the extra-embryonic venous circulation. These results suggest that material properties of the embryonic ventricle are modified after temporarily reduced hemodynamic load...
  92. ncbi request reprint Functional attenuation of UFD1l, a 22q11.2 deletion syndrome candidate gene, leads to cardiac outflow septation defects in chicken embryos
    Chihiro Yamagishi
    Department of Pediatrics, University of Texas, Southwestern Medical Center at Dallas, Dallas 75390 9148, USA
    Pediatr Res 53:546-53. 2003
    ..These data suggest that Ufd1l may play a role in cardiac NCC during conotruncal septation...
  93. ncbi request reprint Nuchal edema and venous-lymphatic phenotype disturbance in human fetuses and mouse embryos with aneuploidy
    Mireille N Bekker
    Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, The Netherlands
    J Soc Gynecol Investig 13:209-16. 2006
    ..We hypothesize that a disturbed lymphatic endothelial differentiation and sac formation causes NE. We investigated endothelial differentiation of the jugular lymphatic system in human and mouse species with NE...
  94. ncbi request reprint Increased NCAM expression and vascular development in trisomy 16 mouse embryos: relationship with nuchal translucency
    Mireille N Bekker
    Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, The Netherlands, and Department of Basic Medical Sciences, Anatomy and Developmental Biology, St George Hospital Medical School, London, UK
    Pediatr Res 58:1222-7. 2005
    ..In conclusion, the interaction between neurogenesis and vasculogenesis is disturbed in the trisomy 16 mouse model, and might be a common denominator in the spectrum of anomalies associated with increased nuchal translucency...
  95. ncbi request reprint Histology of vascular myocardial wall of left atrial body after pulmonary venous incorporation
    Yvonne L Douglas
    Department of Cardio Thoracic Surgery, University Medical Center, University of Groningen, Groningen, The Netherlands
    Am J Cardiol 97:662-70. 2006
    ..A border zone between the LA body and LA appendage is hypothesized to be the left part of the embryonic sinus venosus...