Research Topics
Species | Annemieke GelukSummaryAffiliation: Leiden University Medical Center Country: The Netherlands Publications
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Detail Information
Publications
Lack of immune responses to Mycobacterium tuberculosis DosR regulon proteins following Mycobacterium bovis BCG vaccinationMay Young Lin
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
Infect Immun 75:3523-30. 2007..tuberculosis infection, our findings support the development of vaccination strategies incorporating DosR regulon antigens to complement and improve the current BCG vaccine...
Challenges in immunodiagnostic tests for leprosyAnnemieke Geluk
Leiden University Medical Center LUMC, Department of Infectious Diseases, PO Box 9600, 2300 RC Leiden, The Netherlands 31 71 526 1974 31 71 526 6758
Expert Opin Med Diagn 7:265-74. 2013..Diagnostic tests based on such biomarkers can contribute significantly to early detection of leprosy (reactions) thus helping reduce nerve damage...
Postgenomic Mycobacterium leprae antigens for cellular and serological diagnosis of M. leprae exposure, infection and leprosy diseaseAnnemieke Geluk
Department of Infectious Diseases, Leiden University Medical Center, The Netherlands
Lepr Rev 82:402-21. 2011..Here we provide an overview of the nearly 200 recombinant single proteins that were investigated during the last decade for their potential to be applied in field-friendly tests for the early diagnosis of leprosy...- From genome-based in silico predictions to ex vivo verification of leprosy diagnosisAnnemieke Geluk
Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
Clin Vaccine Immunol 16:352-9. 2009..Future work should concentrate on the development of a sensitive and field-friendly assay and identification of additional peptides and proteins that can induce M. leprae-specific T-cell responses... - HLA and leprosy in the pre and postgenomic erasAnnemieke Geluk
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center
Hum Immunol 67:439-45. 2006..Thus current state-of-the-art HLA immunogenetics can provide new tools for specific diagnosis of M. leprae infection, which can impact our understanding of leprosy transmission and can lead to improved intervention... - Enhancing sensitivity of detection of immune responses to Mycobacterium leprae peptides in whole-blood assaysAnnemieke Geluk
Department of Infectious Diseases, LUMC, P O Box 9600, 2300 RC Leiden, Netherlands
Clin Vaccine Immunol 17:993-1004. 2010..leprae peptide responses, but its production was enhanced by IL-12 nonspecifically. We conclude that IFN-gamma-based WBA combined with IL-12 represents a more sensitive and robust assay for measuring reactivity to M. leprae peptides... - Postgenomic approach to identify novel Mycobacterium leprae antigens with potential to improve immunodiagnosis of infectionAnnemieke Geluk
Department of Immunohematology and Blood Transfusion, LUMC PO Box 9600, 2300 RC Leiden, The Netherlands
Infect Immun 73:5636-44. 2005..leprae-exposed individuals. These proteins may provide new tools to develop tests for specific diagnosis of M. leprae infection and may enhance our understanding of leprosy and its transmission... - Immunological crossreactivity of the Mycobacterium leprae CFP-10 with its homologue in Mycobacterium tuberculosisA Geluk
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Scand J Immunol 59:66-70. 2004..tuberculosis at the T-cell level. This crossreactivity has implications for the use of CFP-10 of these mycobacterial species as diagnostic tool in areas endemic for both the diseases... - Rational combination of peptides derived from different Mycobacterium leprae proteins improves sensitivity for immunodiagnosis of M. leprae infectionAnnemieke Geluk
Department of Infectious Diseases Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Clin Vaccine Immunol 15:522-33. 2008..Thus, this study provides the first evidence for the possibility of immunodiagnostics for leprosy based on mixtures of peptides recognized in the context of different HLA alleles... - Identification and characterization of the ESAT-6 homologue of Mycobacterium leprae and T-cell cross-reactivity with Mycobacterium tuberculosisAnnemieke Geluk
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Infect Immun 70:2544-8. 2002..Moreover, our results suggest that there is significant cross-reactivity between T-ESAT-6 and L-ESAT-6, which has implications for the use of ESAT-6 as tool for diagnosis of leprosy and TB in areas where both diseases are endemic... - Double- and monofunctional CD4⁺ and CD8⁺ T-cell responses to Mycobacterium tuberculosis DosR antigens and peptides in long-term latently infected individualsSusanna Commandeur
Department of Infectious Diseases, Leiden University Medical Centre, Leiden, The Netherlands
Eur J Immunol 41:2925-36. 2011..These findings demonstrate the prominent presence of double- and monofunctional CD4(+) and CD8(+) T-cell responses in naturally protected individuals and support the possibility of designing Mtb DosR antigen-based TB vaccines... - Mycobacterium tuberculosis peptides presented by HLA-E molecules are targets for human CD8 T-cells with cytotoxic as well as regulatory activitySimone A Joosten
Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
PLoS Pathog 6:e1000782. 2010..Our results significantly increase our understanding of the human immune response to Mtb by identification of CD8(+) T-cell responses to novel HLA-E binding peptides of Mtb, which have cytotoxic as well as immunoregulatory activity... - New biomarkers with relevance to leprosy diagnosis applicable in areas hyperendemic for leprosyAnnemieke Geluk
Department of Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, The Netherlands
J Immunol 188:4782-91. 2012..leprae exposure using IFN-γ or IFN-inducible protein-10, and also shows that MCP-1, MIP-1β, and IL-1β can potentially distinguish pathogenic immune responses from those induced during asymptomatic exposure to M. leprae... - Identification of human T-cell responses to Mycobacterium tuberculosis resuscitation-promoting factors in long-term latently infected individualsSusanna Commandeur
Department of Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, Netherlands
Clin Vaccine Immunol 18:676-83. 2011..We conclude that M. tuberculosis Rpf antigens are important targets in the human immune response to M. tuberculosis and represent interesting TB vaccine candidate antigens... - Immunogenicity of Mycobacterium leprae unique antigens in leprosy endemic populations in Asia and AfricaKidist Bobosha
Department of Infectious Diseases, Leiden University Medical Center, The Netherlands
Lepr Rev 82:445-58. 2011..In summary, identification of multiple M. leprae proteins inducing M. leprae-specific T cell responses in groups at high risk of developing leprosy may contribute to improve early detection for M. leprae infection... - Interferon-gamma production in response to M. tuberculosis antigens in TB patients in IndonesiaYanri W Subronto
Dept. of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Adv Exp Med Biol 531:249-60. 2003 - ML1419c peptide immunization induces Mycobacterium leprae-specific HLA-A*0201-restricted CTL in vivo with potential to kill live mycobacteriaAnnemieke Geluk
Department of Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, The Netherlands
J Immunol 187:1393-402. 2011..leprae and B cell help for induction of specific IgG, suggesting its potential use in diagnostics and as a subunit (vaccine) for M. leprae infection... - A multistage-polyepitope vaccine protects against Mycobacterium tuberculosis infection in HLA-DR3 transgenic miceAnnemieke Geluk
Department of Infectious Diseases, Leiden University Medical Centre, The Netherlands
Vaccine 30:7513-21. 2012..Given the extensive repertoire of potential Mtb antigens available for immune recognition, the data of our model demonstrate the potential of multistage-polyepitope vaccines to protect against TB... - An unbiased genome-wide Mycobacterium tuberculosis gene expression approach to discover antigens targeted by human T cells expressed during pulmonary infectionSusanna Commandeur
Department of Infectious Diseases, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
J Immunol 190:1659-71. 2013..tuberculosis-infected individuals, and may therefore represent attractive Ags for new TB vaccines... - T-cell recognition of the HspX protein of Mycobacterium tuberculosis correlates with latent M. tuberculosis infection but not with M. bovis BCG vaccinationAnnemieke Geluk
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
Infect Immun 75:2914-21. 2007..Thus, we suggest that subunit vaccines incorporating HspX and/or other latency antigens, as well as recombinant BCG strains expressing latency antigens need to be considered as new vaccines against TB... - Alternative peptide repertoire of HLA-E reveals a binding motif that is strikingly similar to HLA-A2Margit H Lampen
Department of Clinical Oncology, Leiden University Medical Center, The Netherlands
Mol Immunol 53:126-31. 2013..Our findings suggest that the dominant leader peptides uniquely conform to HLA-E, but that in their absence a peptide pool is presented like that of HLA-A*0201... - Identification of a conserved universal Th epitope in HIV-1 reverse transcriptase that is processed and presented to HIV-specific CD4+ T cells by at least four unrelated HLA-DR moleculesS H van der Burg
Department of Immunohematology and Blood Bank, Leiden University Medical Center, The Netherlands
J Immunol 162:152-60. 1999..Taken together, these data indicate that peptide RT171-190 constitutes an attractive component of vaccines aiming at induction or enhancement of HIV-1-specific T cell immunity... - Identification of a human CD8+ regulatory T cell subset that mediates suppression through the chemokine CC chemokine ligand 4Simone A Joosten
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333 ZA, Leiden, The Netherlands
Proc Natl Acad Sci U S A 104:8029-34. 2007..This CD8+LAG-3+CD25+FoxP3+CCL4+ Treg subset thus may play a role in immunoregulation in humans, including infectious diseases... - Frequent detection of human papillomavirus 16 E2-specific T-helper immunity in healthy subjectsAnnemieke de Jong
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
Cancer Res 62:472-9. 2002.... - Mycobacterium leprae virulence-associated peptides are indicators of exposure to M. leprae in Brazil, Ethiopia and NepalKidist Bobosha
Department of Infectious Diseases, University Medical Center, Leiden, The Netherlands
Mem Inst Oswaldo Cruz 107:112-23. 2012..Therefore, the M. leprae virulence-associated peptides identified in this study may be useful for identifying exposure to M. leprae in population with differing HLA polymorphisms... - Lateral flow assay for simultaneous detection of cellular- and humoral immune responsesPaul L A M Corstjens
Department of Molecular Cell Biology Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands
Clin Biochem 44:1241-6. 2011..The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases... - HLA-DR binding analysis of peptides from islet antigens in IDDMA Geluk
Department of Immunohematology and Blood Bank, Leiden University Medical Center, The Netherlands
Diabetes 47:1594-601. 1998..Furthermore, negative thymic selection of insulin peptides in low-risk (HLA-DR2 expressing) subjects may explain the lack of autoreactivity to insulin in such individuals... - Antigenic equivalence of human T-cell responses to Mycobacterium tuberculosis-specific RD1-encoded protein antigens ESAT-6 and culture filtrate protein 10 and to mixtures of synthetic peptidesS M Arend
Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
Infect Immun 68:3314-21. 2000..tuberculosis, and peptides have the advantage of faster production at lower cost... - Identification of major epitopes of Mycobacterium tuberculosis AG85B that are recognized by HLA-A*0201-restricted CD8+ T cells in HLA-transgenic mice and humansA Geluk
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
J Immunol 165:6463-71. 2000..tuberculosis and identify Ag85B epitopes that are strongly recognized by HLA-A*0201-restricted CD8(+) T cells in humans and mice. These epitopes thus represent potential subunit components for the design of vaccines against tuberculosis... - Natural T-helper immunity against human papillomavirus type 16 (HPV16) E7-derived peptide epitopes in patients with HPV16-positive cervical lesions: identification of 3 human leukocyte antigen class II-restricted epitopesS H van der Burg
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Int J Cancer 91:612-8. 2001..Our combined data argue for more extensive as well as longitudinal analysis of HPV16-specific T-cell immunity using the ELISPOT assay described, as well as for HPV-specific vaccination of individuals with HPV+ lesions... - An HLA-DRB1-derived peptide associated with protection against rheumatoid arthritis is naturally processed by human APCsA Snijders
Immunohematology and Blood Transfusion and Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands
J Immunol 166:4987-93. 2001..These findings support a model in which self-MHC-derived peptide can modulate predisposition to autoimmune disease in humans... - Altered peptide ligands of islet autoantigen Imogen 38 inhibit antigen specific T cell reactivity in human type-1 diabetesA Geluk
Department of Immunohematology and Blood Bank, Leiden University Medical Center, The Netherlands
J Autoimmun 11:353-61. 1998..Thus, the p55-70 APL inhibit beta cell autoantigen-induced activation of an Imogen-reactive T-cell clone derived from an IDDM patient, by acting as partial TCR agonists that inhibit TCR down-modulation... - Identification of HLA class II-restricted determinants of Mycobacterium tuberculosis-derived proteins by using HLA-transgenic, class II-deficient miceA Geluk
Department of Immunohematology and Blood Bank, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
Proc Natl Acad Sci U S A 95:10797-802. 1998..Ab0 mice and HLA-DR3+ humans recognize the same immunodominant determinants. Our data support the use of HLA-transgenic mice in identifying human T cell determinants for the design of new vaccines... - Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic ControlsKidist Bobosha
Department of Infectious Diseases, LUMC, P O Box 9600, 2300 RC Leiden, The Netherlands
J Trop Med 2012:132049. 2012..leprae exposed or infected individuals and can also be used to indicate the magnitude of M. leprae transmission even in the context of various HLA alleles as present in these different genetic backgrounds... - T-cell responses to the Mycobacterium tuberculosis-specific antigen ESAT-6 in Brazilian tuberculosis patientsFernando L L Cardoso
Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil
Infect Immun 70:6707-14. 2002..tuberculosis infection in an area of TB endemicity... - Pulmonary delivery of chitosan-DNA nanoparticles enhances the immunogenicity of a DNA vaccine encoding HLA-A*0201-restricted T-cell epitopes of Mycobacterium tuberculosisMaytal Bivas-Benita
Leiden Amsterdam Center for Drug Research, Division of Pharmaceutical Technology, PO Box 9502, 2300 RA Leiden, The Netherlands
Vaccine 22:1609-15. 2004.... - Identification of specific proteins and peptides in Mycobacterium leprae suitable for the selective diagnosis of leprosyJohn S Spencer
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
J Immunol 175:7930-8. 2005..leprae infection and epidemiological surveys of the incidence of leprosy, of which little is known... - Direct ex vivo detection of HLA-DR3-restricted cytomegalovirus- and Mycobacterium tuberculosis-specific CD4+ T cellsCorine Bronke
Department of Clinical Viro-Immunology, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam
Hum Immunol 66:950-61. 2005..Thus, HLA-DR3/peptide tetrameric molecules can be used to investigate HLA-DR3-restricted antigen-specific CD4+ T cells in clinical disease or after vaccination...