L Dijkshoorn

Summary

Affiliation: Leiden University Medical Center
Country: The Netherlands

Publications

  1. pmc Exploring the evolutionary dynamics of plasmids: the Acinetobacter pan-plasmidome
    Marco Fondi
    Laboratory of Microbial and Molecular Evolution, Dept, of Evolutionary Biology, via Romana 17 19, University of Florence, I 50125 Florence, Italy
    BMC Evol Biol 10:59. 2010
  2. ncbi request reprint Understanding patterns of use and scientific opportunities in the emerging global microbial commons
    Lenie Dijkshoorn
    Department of Infectious Diseases C5 P, Leiden University Medical Center, Albinusdreef 2, P O Box 9600, 2300 RC Leiden, The Netherlands
    Res Microbiol 161:407-13. 2010
  3. ncbi request reprint An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii
    Lenie Dijkshoorn
    Department of Infectious Diseases C5 P, Leiden University Medical Centre, Albinusdreef 2, P O Box 9600, 2300 RC Leiden, The Netherlands
    Nat Rev Microbiol 5:939-51. 2007
  4. ncbi request reprint Prevalence of Acinetobacter baumannii and other Acinetobacter spp. in faecal samples from non-hospitalised individuals
    L Dijkshoorn
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Clin Microbiol Infect 11:329-32. 2005
  5. pmc The synthetic N-terminal peptide of human lactoferrin, hLF(1-11), is highly effective against experimental infection caused by multidrug-resistant Acinetobacter baumannii
    Lenie Dijkshoorn
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Antimicrob Agents Chemother 48:4919-21. 2004
  6. pmc Endemic and epidemic acinetobacter species in a university hospital: an 8-year survey
    P J van den Broek
    Department of Infectious Diseases, C5 P, P O Box 9600, 2300 RC Leiden, The Netherlands
    J Clin Microbiol 47:3593-9. 2009
  7. ncbi request reprint Multifactorial origin of high incidence of Serratia marcescens in a cardio-thoracic ICU: analysis of risk factors and epidemiological characteristics
    M G J de Boer
    Department of Infectious Diseases, Leiden University Medical Center, P O Box 9600, Leiden, The Netherlands
    J Infect 56:446-53. 2008
  8. ncbi request reprint Epidemiology of multiple Acinetobacter outbreaks in The Netherlands during the period 1999-2001
    P J van den Broek
    Department of Infectious Diseases, Leiden University Medical Centre, Leiden, The Netherlands
    Clin Microbiol Infect 12:837-43. 2006
  9. doi request reprint Dissemination of Bacillus cereus in a paediatric intensive care unit traced to insufficient disinfection of reusable ventilator air-flow sensors
    J S Kalpoe
    Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
    J Hosp Infect 68:341-7. 2008
  10. ncbi request reprint Methicillin-resistant Staphylococcus aureus and Acinetobacter baumannii: an unexpected difference in epidemiologic behavior
    A T Bernards
    Department of Medical Microbiology, Leiden University Medical Centre, Leiden, The Netherlands
    Am J Infect Control 26:544-51. 1998

Collaborators

Detail Information

Publications44

  1. pmc Exploring the evolutionary dynamics of plasmids: the Acinetobacter pan-plasmidome
    Marco Fondi
    Laboratory of Microbial and Molecular Evolution, Dept, of Evolutionary Biology, via Romana 17 19, University of Florence, I 50125 Florence, Italy
    BMC Evol Biol 10:59. 2010
    ..Given their importance for conferring adaptive traits to the host organisms, the interest in plasmid sequencing is growing and now many complete plasmid sequences are available online...
  2. ncbi request reprint Understanding patterns of use and scientific opportunities in the emerging global microbial commons
    Lenie Dijkshoorn
    Department of Infectious Diseases C5 P, Leiden University Medical Center, Albinusdreef 2, P O Box 9600, 2300 RC Leiden, The Netherlands
    Res Microbiol 161:407-13. 2010
    ....
  3. ncbi request reprint An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii
    Lenie Dijkshoorn
    Department of Infectious Diseases C5 P, Leiden University Medical Centre, Albinusdreef 2, P O Box 9600, 2300 RC Leiden, The Netherlands
    Nat Rev Microbiol 5:939-51. 2007
    ..Here, we present an overview of the current knowledge of the genus Acinetobacter, with the emphasis on the clinically most important species, Acinetobacter baumannii...
  4. ncbi request reprint Prevalence of Acinetobacter baumannii and other Acinetobacter spp. in faecal samples from non-hospitalised individuals
    L Dijkshoorn
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Clin Microbiol Infect 11:329-32. 2005
    ..The predominant species were Acinetobactor johnsonii and genomic sp. 11, which were cultured from 22 and five specimens, respectively. A. baumannii did not seem to be widespread in the faecal flora of individuals in the community...
  5. pmc The synthetic N-terminal peptide of human lactoferrin, hLF(1-11), is highly effective against experimental infection caused by multidrug-resistant Acinetobacter baumannii
    Lenie Dijkshoorn
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Antimicrob Agents Chemother 48:4919-21. 2004
    ..Therefore, this peptide is a promising candidate as a novel agent against infections with multidrug-resistant A. baumannii...
  6. pmc Endemic and epidemic acinetobacter species in a university hospital: an 8-year survey
    P J van den Broek
    Department of Infectious Diseases, C5 P, P O Box 9600, 2300 RC Leiden, The Netherlands
    J Clin Microbiol 47:3593-9. 2009
    ..There was a borderline significant (P = 0.059) trend of decreasing susceptibility. A. baumannii was the Acinetobacter species causing the largest burden of multiple-antibiotic resistance and transmissions in the hospital...
  7. ncbi request reprint Multifactorial origin of high incidence of Serratia marcescens in a cardio-thoracic ICU: analysis of risk factors and epidemiological characteristics
    M G J de Boer
    Department of Infectious Diseases, Leiden University Medical Center, P O Box 9600, Leiden, The Netherlands
    J Infect 56:446-53. 2008
    ..A four-fold increase in the incidence of Serratia marcescens occurred in a cardio-thoracic ICU within a 13-month period. Clinical, epidemiological and molecular characteristics were analysed to elucidate the outbreak's origin...
  8. ncbi request reprint Epidemiology of multiple Acinetobacter outbreaks in The Netherlands during the period 1999-2001
    P J van den Broek
    Department of Infectious Diseases, Leiden University Medical Centre, Leiden, The Netherlands
    Clin Microbiol Infect 12:837-43. 2006
    ..In all hospitals, a large panel of measures was required to bring the outbreak to an end. Extensive environmental sampling yielded numerous positive samples in most but not all hospitals...
  9. doi request reprint Dissemination of Bacillus cereus in a paediatric intensive care unit traced to insufficient disinfection of reusable ventilator air-flow sensors
    J S Kalpoe
    Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
    J Hosp Infect 68:341-7. 2008
    ..Disinfection of all air-flow-guiding reusable parts for mechanical ventilation should be addressed with great care and should include effective autoclaving in order to eradicate spores...
  10. ncbi request reprint Methicillin-resistant Staphylococcus aureus and Acinetobacter baumannii: an unexpected difference in epidemiologic behavior
    A T Bernards
    Department of Medical Microbiology, Leiden University Medical Centre, Leiden, The Netherlands
    Am J Infect Control 26:544-51. 1998
    ..The airborne mode of spread of acinetobacters should be taken into account, and guidelines should be revised accordingly...
  11. doi request reprint Can Escherichia coli be used as an indicator organism for transmission events in hospitals?
    P J van den Broek
    Department of Infectious Diseases, C5 P, Leiden University Medical Centre, P O Box 9600, 2300 RC, Leiden, The Netherlands
    Eur J Clin Microbiol Infect Dis 28:169-73. 2009
    ..This study identified a number of problems regarding the use of Escherichia coli as indicator organism for transmission events. The use of Escherichia coli for this purpose cannot be recommended at the moment...
  12. pmc Performance of phenotypic and genotypic methods to determine the clinical relevance of serial blood isolates of staphylococcus epidermidis in patients with septicemia
    J H Sloos
    Departments of Medical Microbiology and Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    J Clin Microbiol 38:2488-93. 2000
    ..For 6 of the 11 sets, the results of all methods except those of biotyping corresponded completely. Quantitative antibiogram typing, PFGE, and AFLP proved to be the most accurate of the six typing methods tested...
  13. ncbi request reprint Biofilm production by Staphylococcus epidermidis isolates associated with catheter related bacteremia
    L Vogel
    Department of Medical Microbiology, Leiden University Medical Center, L4 P, P O Box 9600, 2300 RC Leiden, Leiden, The Netherlands
    Diagn Microbiol Infect Dis 36:139-41. 2000
    ..This difference was due to seven catheter related isolates. These findings do not show a clear association between biofilm production and virulence...
  14. ncbi request reprint Acinetobacter junii causes life-threatening sepsis in preterm infants
    A J de Beaufort
    Department of Paediatrics, Leiden University Medical Center, The Netherlands
    Acta Paediatr 88:772-5. 1999
    ..Contaminated intravenous fat emulsion is implicated as a possible source of the infection. As a part of rigid infection control, intravenous feedings should be prepared under aseptic conditions...
  15. ncbi request reprint Persistent Acinetobacter baumannii? Look inside your medical equipment
    A T Bernards
    Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
    Infect Control Hosp Epidemiol 25:1002-4. 2004
    ..Removing dust from the machines and replacing all dust filters brought the outbreaks to an end...
  16. ncbi request reprint Epidemiologic typing of Escherichia coli using RAPD analysis, ribotyping and serotyping
    L Vogel
    Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
    Clin Microbiol Infect 6:82-7. 2000
    ..To compare random amplified polymorphic DNA (RAPD) analysis and ribotyping with serotyping for epidemiologic typing of Escherichia coli...
  17. ncbi request reprint High resolution DNA fingerprinting by AFLP to study the genetic variation among Oesophagostomum bifurcum (Nematoda) from human and non-human primates from Ghana
    J M de Gruijter
    Department of Parasitology, Leiden University Medical Center, University of Leiden, PO Box 9600, 2300 RC Leiden, The Netherlands
    Parasitology 130:229-37. 2005
    ..The results demonstrated the effectiveness of the present AFLP method for establishing genetic variation within O. bifurcum, and indicates its applicability to other parasitic nematodes of human and/or veterinary health importance...
  18. doi request reprint CsuA/BABCDE-dependent pili are not involved in the adherence of Acinetobacter baumannii ATCC19606(T) to human airway epithelial cells and their inflammatory response
    Anna de Breij
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Res Microbiol 160:213-8. 2009
    ..baumannii ATCC19606(T) to bronchial epithelial cells. The structure of the short pili and their possible role in adherence to human cells requires further investigation...
  19. pmc Do biofilm formation and interactions with human cells explain the clinical success of Acinetobacter baumannii?
    Anna de Breij
    Department of Infectious Diseases, Leiden University Medical Centre, Leiden, The Netherlands
    PLoS ONE 5:e10732. 2010
    ..Despite numerous reports documenting its epidemicity, little is known about the pathogenicity of A. baumannii. The aim of this study was to obtain insight into the factors that might explain the clinical success of A. baumannii...
  20. pmc Characterization of toxin A-negative, toxin B-positive Clostridium difficile isolates from outbreaks in different countries by amplified fragment length polymorphism and PCR ribotyping
    Renate J van den Berg
    Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    J Clin Microbiol 42:1035-41. 2004
    ..001, chi-square test). We conclude that clindamycin-resistant C. difficile toxin A(-)/B(+) strain (PCR ribotype 017/20, AFLP type 20, serogroup F) has a clonal worldwide spread...
  21. ncbi request reprint AFLP fingerprinting for the analysis of genetic diversity within Necator americanus
    Johanna M de Gruijter
    Leiden University Medical Center, University of Leiden, PO Box 9600, 2300 RC Leiden, The Netherlands
    Mol Cell Probes 20:317-21. 2006
    ..The results demonstrated the usefulness of AFLP fingerprinting for establishing genetic variation within N. americanus and reinforce its applicability to other parasitic helminths of human and/or veterinary health importance...
  22. ncbi request reprint Repetitive-DNA-element PCR fingerprinting and antibiotic resistance of pan-European multi-resistant Acinetobacter baumannii clone III strains
    Geert Huys
    Laboratory of Microbiology and BCCM LMG Bacteria Collection, Ghent University, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
    J Med Microbiol 54:851-6. 2005
    ....
  23. pmc Oligonucleotide array-based identification of species in the Acinetobacter calcoaceticus-A. baumannii complex in isolates from blood cultures and antimicrobial susceptibility testing of the isolates
    Wen Chien Ko
    Department of Internal Medicine, Center of Infection Control, National Cheng Kung University Hospital, Tainan, Taiwan
    J Clin Microbiol 46:2052-9. 2008
    ..calcoaceticus-A. baumannii complex emphasize that the differentiation of species within the complex is relevant from a clinical-epidemiological point of view...
  24. ncbi request reprint Diversity of aminoglycoside-resistance genes and their association with class 1 integrons among strains of pan-European Acinetobacter baumannii clones
    Alexandr Nemec
    National Institute of Public Health, Srobarova 48, 100 42 Prague 10, Czech Republic
    J Med Microbiol 53:1233-40. 2004
    ..baumannii...
  25. ncbi request reprint Emergence of carbapenem resistance in Acinetobacter baumannii in the Czech Republic is associated with the spread of multidrug-resistant strains of European clone II
    Alexandr Nemec
    Centre of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
    J Antimicrob Chemother 62:484-9. 2008
    ..The aim of this study was to analyse the emergence of carbapenem resistance among hospital strains of Acinetobacter in the Czech Republic...
  26. ncbi request reprint Distribution of tetracycline resistance genes in genotypically related and unrelated multiresistant Acinetobacter baumannii strains from different European hospitals
    Geert Huys
    Laboratory of Microbiology, Ghent University, K L Ledeganckstraat 35, Ghent, Belgium
    Res Microbiol 156:348-55. 2005
    ..Collectively, our phenotypic and genotypic resistance data support the therapeutic evaluation of second-generation tetracyclines like MIN as promising agents for treating MDRAB infections...
  27. ncbi request reprint Identification of a new geographically widespread multiresistant Acinetobacter baumannii clone from European hospitals
    Helke van Dessel
    Eijkman Winkler Center for Microbiology, Infectious Diseases and Inflammation, University Medical Center Utrecht, AZU G04 614, P O Box 85500, 3508 GA, Utrecht, The Netherlands
    Res Microbiol 155:105-12. 2004
    ....
  28. pmc Comparison of Acinetobacter baumannii isolates from United Kingdom hospitals with predominant Northern European genotypes by amplified-fragment length polymorphism analysis
    Richard P Spence
    Department of Microbiology, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom
    J Clin Microbiol 42:832-4. 2004
    ..Two predominant northern European genotypes associated with outbreaks in the mid-1980s had been superseded by new outbreak-associated genotypes...
  29. ncbi request reprint Long-term predominance of two pan-European clones among multi-resistant Acinetobacter baumannii strains in the Czech Republic
    Alexandr Nemec
    National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic
    J Med Microbiol 53:147-53. 2004
    ....
  30. ncbi request reprint Relationship between the AdeABC efflux system gene content, netilmicin susceptibility and multidrug resistance in a genotypically diverse collection of Acinetobacter baumannii strains
    Alexandr Nemec
    Centre of Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic
    J Antimicrob Chemother 60:483-9. 2007
    ..To assess the occurrence of the genes of the AdeABC efflux system and their association with antimicrobial resistance in Acinetobacter baumannii...
  31. pmc Horizontal gene transfer in a polyclonal outbreak of carbapenem-resistant Acinetobacter baumannii
    Jubelle K Valenzuela
    Centre for Infectious Diseases and Microbiology, University of Sydney, New South Wales, Australia
    J Clin Microbiol 45:453-60. 2007
    ..Epidemiological investigation of major outbreaks should include whole-genome typing as well as analysis of potentially transmissible resistance genes and their vehicles...
  32. pmc Standardization and interlaboratory reproducibility assessment of pulsed-field gel electrophoresis-generated fingerprints of Acinetobacter baumannii
    Harald Seifert
    Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Germany
    J Clin Microbiol 43:4328-35. 2005
    ..baumannii strains at different locations with sufficient interlaboratory reproducibility to set up an electronic database to monitor the geographic spread of epidemic strains...
  33. ncbi request reprint Rapid epidemiological analysis of Acinetobacter strains by Raman spectroscopy
    Kees Maquelin
    Erasmus MC, Department General Surgery, Center for Optical Diagnostics and Therapy, Room Wk 331, P O Box 2040, Rotterdam 3000 CA, The Netherlands
    J Microbiol Methods 64:126-31. 2006
    ..We conclude that Raman spectroscopy could be an easy-to-use alternative in epidemiological studies of Acinetobacter strains and a promising starting point for the development of epidemiological studies in general...
  34. ncbi request reprint High genotypic diversity of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis in the Czech Republic
    Sarka Vosahlikova
    Department of Paediatrics, 2nd Medical School, Charles University in Prague, Uvalu 84, 15006 Prague 5, Czech Republic
    Res Microbiol 158:324-9. 2007
    ..The absence of highly epidemic P. aeruginosa strains may result from infection control measures taken upon recognition of the epidemic B. cenocepacia...
  35. ncbi request reprint Acinetobacter parvus sp. nov., a small-colony-forming species isolated from human clinical specimens
    Alexandr Nemec
    National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic
    Int J Syst Evol Microbiol 53:1563-7. 2003
    ....
  36. ncbi request reprint Adherence of Acinetobacter baumannii strains to human bronchial epithelial cells
    Je Chul Lee
    Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, 101, Dongin 2 ga, Jung Gu, Daegu 700 422, South Korea
    Res Microbiol 157:360-6. 2006
    ..This study showed that A. baumannii adheres to human bronchial epithelial cells in vitro and that A. baumannii strains of clone II had a relatively high capacity for adhering to these cells...
  37. pmc Naturally transformable Acinetobacter sp. strain ADP1 belongs to the newly described species Acinetobacter baylyi
    Mario Vaneechoutte
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Blok A, De Pintelaan 185, 9000 Gent, Belgium
    Appl Environ Microbiol 72:932-6. 2006
    ....
  38. pmc Lack of evidence for "Acinetobacter septicus" as a species different from Acinetobacter ursingii?
    Alexandr Nemec
    J Clin Microbiol 46:2826-7; author reply 2827. 2008
  39. ncbi request reprint Intercenter reproducibility of binary typing for Staphylococcus aureus
    Willem B van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 51:19-28. 2002
    ..The current BT protocol thus represents a simple method generating robust, reproducible genotype data for S. aureus strains...
  40. pmc Nosocomial bacteremia due to an as yet unclassified acinetobacter genomic species 17-like strain
    Jesús Rodríguez-Baño
    Department of Microbiology, School of Medicine, University of Barcelona, Hospital Clinic, IDIBAPS, Villarroel, 170, 08036 Barcelona, Spain
    J Clin Microbiol 44:1587-9. 2006
    ..baumannii epidemic...
  41. doi request reprint Reclassification of Acinetobacter grimontii Carr et al. 2003 as a later synonym of Acinetobacter junii Bouvet and Grimont 1986
    Mario Vaneechoutte
    Department of Clinical Chemistry, Microbiology and Immunology, University Hospital, Blok A, B 9000 Gent, Belgium
    Int J Syst Evol Microbiol 58:937-40. 2008
    ..These findings were corroborated for a second representative of the A. grimontii type strain, DSM 14968T. These data confirm that Acinetobacter grimontii is a later heterotypic synonym of Acinetobacter junii...
  42. ncbi request reprint Differential roles of CD14 and toll-like receptors 4 and 2 in murine Acinetobacter pneumonia
    Sylvia Knapp
    Laboratory of Experimental Internal Medicine Academic Medical Center, University of Amsterdam, Meibergdreef 9, G2 132, 1105 AZ Amsterdam, The Netherlands
    Am J Respir Crit Care Med 173:122-9. 2006
    ..baumannii infection have not been elucidated...
  43. pmc Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region
    Hsien Chang Chang
    Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan
    J Clin Microbiol 43:1632-9. 2005
    ..In conclusion, ITS sequence-based identification is reliable and provides a promising tool for elucidation of the clinical significance of the different species of the A. calcoaceticus-A. baumannii complex...
  44. ncbi request reprint Characterization of a 2.6 kbp variable region within a class 1 integron found in an Acinetobacter baumannii strain isolated from a horse
    Yvonne Abbott
    Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
    J Antimicrob Chemother 55:367-70. 2005
    ..A complete gene cassette contained in a class 1 integron from a multidrug-resistant (MDR) isolate of Acinetobacter baumannii cultured from a horse was characterized by molecular methods...