Frank de Gruijl

Summary

Affiliation: Leiden University Medical Center
Country: The Netherlands

Publications

  1. doi request reprint The effects of a mid-winter 8-week course of sub-sunburn sunbed exposures on tanning, vitamin D status and colds
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center LUMC, Leiden, The Netherlands
    Photochem Photobiol Sci 11:1848-54. 2012
  2. pmc Molecular profiling of cutaneous squamous cell carcinomas and actinic keratoses from organ transplant recipients
    Liesbeth Hameetman
    Department of Toxicogenetics, Leiden University Medical Center, PO Box 9600, 2300, RC Leiden, The Netherlands
    BMC Cancer 13:58. 2013
  3. ncbi request reprint UV-induced DNA damage, repair, mutations and oncogenic pathways in skin cancer
    F R de Gruijl
    Department of Dermatology, Sylvius Lab, Leiden Univ Med Ctr, Wassenaarseweg 72, NL 2333 AL Leiden, The Netherlands
    J Photochem Photobiol B 63:19-27. 2001
  4. ncbi request reprint Elimination of keratinocytes stagnant in s-phase through epidermal turnover instead of in situ apoptosis
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    Cell Cycle 5:565-6. 2006
  5. doi request reprint UV-induced immunosuppression in the balance
    Frank R de Gruijl
    Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    Photochem Photobiol 84:2-9. 2008
  6. doi request reprint Early events in UV carcinogenesis--DNA damage, target cells and mutant p53 foci
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    Photochem Photobiol 84:382-7. 2008
  7. ncbi request reprint Photocarcinogenesis: UVA vs. UVB radiation
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center LUMC, Leiden, Netherlands
    Skin Pharmacol Appl Skin Physiol 15:316-20. 2002
  8. ncbi request reprint Ultraviolet radiation and tumor immunity
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center LUMC, Sylvius Laboratories, Room 3038, Wassenaarseweg 72, NL 2333 AL Leiden, The Netherlands
    Methods 28:122-9. 2002
  9. ncbi request reprint Physical variables in experimental photocarcinogenesis and quantitative relationships between stages of tumor development
    Frank R de Gruijl
    Dermatology, Leiden Univ Med Ctr LUMC, Sylvius Lab, Wassenaarseweg 72, NL 2333 AL Leiden, Netherlands
    Front Biosci 7:d1525-30. 2002
  10. doi request reprint Protein kinase Cepsilon reveals importance of extrinsic apoptosis in preventing UV carcinogenesis
    Frank de Gruijl
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    J Invest Dermatol 129:1853-6. 2009

Collaborators

Detail Information

Publications50

  1. doi request reprint The effects of a mid-winter 8-week course of sub-sunburn sunbed exposures on tanning, vitamin D status and colds
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center LUMC, Leiden, The Netherlands
    Photochem Photobiol Sci 11:1848-54. 2012
    ..Hence, the sub-sunburn sunbed treatment was effective in tanning and increasing the 25(OH)D serum level, more so than 1000 IU per day, but had no appreciable effect on colds...
  2. pmc Molecular profiling of cutaneous squamous cell carcinomas and actinic keratoses from organ transplant recipients
    Liesbeth Hameetman
    Department of Toxicogenetics, Leiden University Medical Center, PO Box 9600, 2300, RC Leiden, The Netherlands
    BMC Cancer 13:58. 2013
    ..The aim of this genome-wide study was to identify pathways and genomic alterations that are consistently involved in the formation of SCCs and their precursor lesions, actinic keratoses (AKs)...
  3. ncbi request reprint UV-induced DNA damage, repair, mutations and oncogenic pathways in skin cancer
    F R de Gruijl
    Department of Dermatology, Sylvius Lab, Leiden Univ Med Ctr, Wassenaarseweg 72, NL 2333 AL Leiden, The Netherlands
    J Photochem Photobiol B 63:19-27. 2001
    ..Solar UVB radiation is most effective in causing these point mutations. Other forms of UV-induced genetic changes (e.g., deletions) may, however, contribute to skin carcinogenesis with different wavelength dependencies...
  4. ncbi request reprint Elimination of keratinocytes stagnant in s-phase through epidermal turnover instead of in situ apoptosis
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    Cell Cycle 5:565-6. 2006
  5. doi request reprint UV-induced immunosuppression in the balance
    Frank R de Gruijl
    Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    Photochem Photobiol 84:2-9. 2008
    ..This premise has fruitfully rekindled the research on this common "sun allergy," affecting to widely varying degrees about one in five Europeans with indoor professions...
  6. doi request reprint Early events in UV carcinogenesis--DNA damage, target cells and mutant p53 foci
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    Photochem Photobiol 84:382-7. 2008
    ..Careful examination of archival material showed that although most small p53 patches arise interfollicularly, some may actually arise high up in a follicle, in the infundibulum...
  7. ncbi request reprint Photocarcinogenesis: UVA vs. UVB radiation
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center LUMC, Leiden, Netherlands
    Skin Pharmacol Appl Skin Physiol 15:316-20. 2002
    ..Both UVB and UVA radiation can give rise to ROS-related point mutations (e.g. G to T) and crude genomic alterations (e.g. deletions) which may not be recognized as caused by UV radiation...
  8. ncbi request reprint Ultraviolet radiation and tumor immunity
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center LUMC, Sylvius Laboratories, Room 3038, Wassenaarseweg 72, NL 2333 AL Leiden, The Netherlands
    Methods 28:122-9. 2002
    ..The latter cells are CD1-restricted, which indicates the importance of "unconventional" antigens to UV-induced tumor tolerance...
  9. ncbi request reprint Physical variables in experimental photocarcinogenesis and quantitative relationships between stages of tumor development
    Frank R de Gruijl
    Dermatology, Leiden Univ Med Ctr LUMC, Sylvius Lab, Wassenaarseweg 72, NL 2333 AL Leiden, Netherlands
    Front Biosci 7:d1525-30. 2002
    ..This disparity between the dose-time relationships for the tumor tolerance and the tumors needs to be investigated further. It could imply a shift to non-immunogenic tumors at low daily doses...
  10. doi request reprint Protein kinase Cepsilon reveals importance of extrinsic apoptosis in preventing UV carcinogenesis
    Frank de Gruijl
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    J Invest Dermatol 129:1853-6. 2009
    ..Protein kinase Cvarepsilon lowers UV-induced expression of Fas and its downstream proteins in this extrinsic route, suppressing apoptosis and enhancing UV carcinogenesis...
  11. doi request reprint Sufficient vitamin D from casual sun exposure?
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center LUCM, Leiden, The Netherlands
    Photochem Photobiol 87:598-601. 2011
    ..This result is an improvement over earlier estimates and shows that casual mid-day summer sun exposure should indeed suffice...
  12. ncbi request reprint Relationship between UV-induced mutant p53 patches and skin tumours, analysed by mutation spectra and by induction kinetics in various DNA-repair-deficient mice
    Heggert Rebel
    Department of Dermatology, Leiden University Medical Centre, Sylvius Laboratory, Leiden, The Netherlands
    Carcinogenesis 26:2123-30. 2005
    ....
  13. ncbi request reprint Enhanced DDB2 expression protects mice from carcinogenic effects of chronic UV-B irradiation
    Sergey Alekseev
    Department of Toxicogenetics, Leiden University Medical Center, Leiden University, Leiden, The Netherlands
    Cancer Res 65:10298-306. 2005
    ..Our results show an important role for DDB2 in the protection against UV-induced cancer and indicate that this protection is most likely mediated by accelerating the repair of photolesions...
  14. pmc Epidermal transit of replication-arrested, undifferentiated keratinocytes in UV-exposed XPC mice: an alternative to in situ apoptosis
    Gerdine J Stout
    Department of Dermatology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
    Proc Natl Acad Sci U S A 102:18980-5. 2005
    ..This expulsion apparently constitutes an alternative route, different from in situ apoptosis, to eliminate DNA-damaged arrested cells from the epidermis...
  15. ncbi request reprint Association of transcription-coupled repair but not global genome repair with ultraviolet-B-induced Langerhans cell depletion and local immunosuppression
    Wendy Kölgen
    Department of Dermatology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Invest Dermatol 121:751-6. 2003
    ..In combination with earlier experiments, it can be concluded that Langerhans cell disappearance is related to ultraviolet-B-induced local but not to systemic immunosuppression...
  16. doi request reprint Differential activity of UV-DDB in mouse keratinocytes and fibroblasts: impact on DNA repair and UV-induced skin cancer
    Alex Pines
    Department of Toxicogenetics, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    DNA Repair (Amst) 8:153-61. 2009
    ..Hence, our observations show that mouse keratinocytes express sufficient levels of UV-DDB for efficient repair of photolesions and efficient protection against UV-induced skin cancer at physiological relevant UV exposure...
  17. ncbi request reprint Mismatch repair protein Msh2 contributes to UVB-induced cell cycle arrest in epidermal and cultured mouse keratinocytes
    Marijke van Oosten
    Department of Toxicogenetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
    DNA Repair (Amst) 4:81-9. 2005
    ..This indicates that MMR plays a role in the UVB-induced S-phase arrest. Alternatively, Msh2 may have a more direct signalling function...
  18. ncbi request reprint Growth stimulation of UV-induced DNA damage retaining epidermal basal cells gives rise to clusters of p53 overexpressing cells
    Joanne G W Nijhof
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    DNA Repair (Amst) 6:1642-50. 2007
    ....
  19. doi request reprint An in vitro three-dimensional model of primary human cutaneous squamous cell carcinoma
    Suzan Commandeur
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    Exp Dermatol 18:849-56. 2009
    ..This model may provide a platform to develop refined preventive and curative treatments and thereby gain understanding of SCC pathogenesis...
  20. pmc Error-prone translesion replication of damaged DNA suppresses skin carcinogenesis by controlling inflammatory hyperplasia
    Anastasia Tsaalbi-Shtylik
    Department of Toxicogenetics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Proc Natl Acad Sci U S A 106:21836-41. 2009
    ..In conclusion, error-prone TLS suppresses tumor-promoting activities of UV light, thereby controlling skin carcinogenesis...
  21. ncbi request reprint Epidermal langerhans cell depletion after artificial ultraviolet B irradiation of human skin in vivo: apoptosis versus migration
    Wendy Kölgen
    Department of Dermatology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Invest Dermatol 118:812-7. 2002
    ..Ultraviolet B-induced Langerhans cell disappearance appears to be mainly attributable to migration...
  22. ncbi request reprint UVB-induced leucocyte trafficking in the epidermis of photosensitive lupus erythematosus patients: normal depletion of Langerhans cells
    Artiena Soe Janssens
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    Exp Dermatol 14:138-42. 2005
    ..In the present study, we tested this hypothesis by investigating leucocyte trafficking in the initial phase of cutaneous LE after intense UVB exposure...
  23. ncbi request reprint Suppression of UV carcinogenesis by difluoromethylornithine in nucleotide excision repair-deficient Xpa knockout mice
    Heggert Rebel
    Department of Dermatology, Leiden University Medical Center, Sylvius Laboratory, 2300 RA, Leiden, The Netherlands
    Cancer Res 62:1338-42. 2002
    ..These results indicate that DFMO suppresses the outgrowth but not the initiation of UV-induced tumors. The DFMO treatment reduced the tumor load but did not offer the Xpa knockout mice full protection against UV carcinogenesis...
  24. ncbi request reprint Epidermal stem and progenitor cells in murine epidermis accumulate UV damage despite NER proficiency
    Joanne G W Nijhof
    Department of Dermatology, Leiden University Medical Center, PO Box 9600, 2300 RC, Leiden, The Netherlands
    Carcinogenesis 28:792-800. 2007
    ..These findings provide the first evidence that epidermal stem and progenitor cells are prone to the accumulation of UV-induced DNA-damage and can be a prominent target in skin carcinogenesis...
  25. ncbi request reprint More epidermal p53 patches adjacent to skin carcinomas in renal transplant recipients than in immunocompetent patients: the role of azathioprine
    Ymke G L de Graaf
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    Exp Dermatol 17:349-55. 2008
    ..011). UV-induced UDS in humans is dominated by repair of cyclobutane pyrimidine dimers, and these DNA lesions can lead to 'UV-signature' mutations in the P53 gene, giving rise to p53 patches...
  26. ncbi request reprint p53 mutations as a marker of skin cancer risk: comparison of UVA and UVB effects
    Frank R de Gruijl
    Department of Dermatology, Sylvius Laboratories, Room 3038, Leiden University Medical Center, Wassenaarseweg 72, NL 2333 Leiden, The Netherlands
    Exp Dermatol 11:37-9. 2002
    ..The number of these clusters at a certain time-point appears to be predictive of the tumor risk at latter time-points. These UVB-induced p53 clusters appear to be suitable surrogates of tumors in short-term experiments...
  27. ncbi request reprint Susceptibility to UV-A and UV-B provocation does not correlate with disease severity of polymorphic light eruption
    Artiena Soe Janssens
    Department of Dermatology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Arch Dermatol 143:599-604. 2007
    ..To examine whether the ease of disease provocation by UV-A and/or UV-B radiation correlates with clinical features of polymorphic light eruption (PLE), including those indicative of disease severity...
  28. doi request reprint Specific betapapillomaviruses associated with squamous cell carcinoma of the skin inhibit UVB-induced apoptosis of primary human keratinocytes
    Linda Struijk
    Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
    J Gen Virol 89:2303-14. 2008
    ..Infections with HPV8 and HPV20 may therefore augment the carcinogenic effect of UV radiation and potentially contribute to oncogenic transformation of the skin...
  29. ncbi request reprint Transcription-coupled repair: impact on UV-induced mutagenesis in cultured rodent cells and mouse skin tumors
    Albert A Van Zeeland
    Department of Toxicogenetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
    Mutat Res 577:170-8. 2005
    ..We speculate that the UVB-induced cancer susceptibility of Csb(-/-) mice is related to the absence of TCR as well as to a lack of a compensating global genome repair system for CPDs in mice...
  30. ncbi request reprint UV exposure, genetic targets in melanocytic tumors and transgenic mouse models
    Frank R de Gruijl
    Department of Dermatology, Leiden University Medical Center LUMC, Sylvius Labs, Room 3038, Wassenaarseweg 72, NL 2333 AL Leiden, The Netherlands
    Photochem Photobiol 81:52-64. 2005
    ..e. neonatal versus adult and chronic versus intermittent overexposure. Intermittent UV-B overexposure deserves special attention because it most strongly evokes proliferative responses in melanocytes...
  31. ncbi request reprint Sunlight exposure and (sero)prevalence of epidermodysplasia verruciformis-associated human papillomavirus
    Fabian Termorshuizen
    Leiden University Medical Center, The Netherlands
    J Invest Dermatol 122:1456-62. 2004
    ..The favorable association between lifetime sun exposure and HPV prevalence, however, underscores the enigmatic role of HPV in skin carcinogenesis...
  32. ncbi request reprint Health effects from stratospheric ozone depletion and interactions with climate change
    Frank R de Gruijl
    Leiden University Medical Centre, Sylvius Lab, Wassenaarseweg 72, NL 2333 AL Leiden, The Netherlands
    Photochem Photobiol Sci 2:16-28. 2003
    ....
  33. ncbi request reprint The influence of painful sunburns and lifetime sun exposure on the risk of actinic keratoses, seborrheic warts, melanocytic nevi, atypical nevi, and skin cancer
    Cornelis Kennedy
    Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
    J Invest Dermatol 120:1087-93. 2003
    ..Neither painful sunburns nor lifetime sun exposure were associated with an increased risk of seborrheic warts...
  34. ncbi request reprint Differential expression of cytokines in UV-B-exposed skin of patients with polymorphous light eruption: correlation with Langerhans cell migration and immunosuppression
    Wendy Kölgen
    Department of Dermatology, University Medical Center Utrecht, Utrecht
    Arch Dermatol 140:295-302. 2004
    ..Disturbances in UV-induced Langerhans cell migration and T helper (T(H)) 2 cell responses could be early steps in the pathogenesis of PLE...
  35. ncbi request reprint Powerful skin cancer protection by a CPD-photolyase transgene
    Judith Jans
    Department of Genetics, Medical Genetics Center, Center for Biomedical Genetics, Erasmus University Medical Center, Rotterdam, Post Office Box 1738, 3000 DR Rotterdam, The Netherlands
    Curr Biol 15:105-15. 2005
    ....
  36. ncbi request reprint Dose-dependent effects of UVB-induced skin carcinogenesis in hairless p53 knockout mice
    Henk J van Kranen
    National Institute of Public Health and Environment, Laboratory of Toxicology, Pathology and Genetics, Department of Carcinogenesis Mutagenesis and Aging, Bilthoven, The Netherlands
    Mutat Res 571:81-90. 2005
    ....
  37. ncbi request reprint p53-specific serum antibodies are not associated with a history of skin carcinoma in renal transplant recipients and immunocompetent individuals
    Ymke G L de Graaf
    J Dermatol Sci 38:228-30. 2005
  38. ncbi request reprint Immunochemical study of DNA modifications in the nuclei of UV-damaged lymphocytes
    Sergei A Snopov
    Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky Ave 4, 194064 St Petersburg, Russia
    Photochem Photobiol Sci 3:85-90. 2004
    ..Thus, unstimulated human lymphocytes appear to be capable of performing pre-incision steps for removal of these DNA lesions...
  39. doi request reprint Climate change and human skin cancer
    Jan C van der Leun
    Ecofys Netherlands, PO Box 8408, NL 3503, RK, Utrecht, The Netherlands
    Photochem Photobiol Sci 7:730-3. 2008
    ....
  40. ncbi request reprint Single UVB overexposure stimulates melanocyte proliferation in murine skin, in contrast to fractionated or UVA-1 exposure
    Arne van Schanke
    Dermatology Department, University Medical Center Utrecht, Utrecht, The Netherlands
    J Invest Dermatol 124:241-7. 2005
    ..Consequently, murine nevi and melanoma are expected to be most effectively induced by intermittent UVB overexposures...
  41. ncbi request reprint Cytokine polymorphisms play a role in susceptibility to ultraviolet B-induced modulation of immune responses after hepatitis B vaccination
    Annemarie Sleijffers
    National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    J Immunol 170:3423-8. 2003
    ..This study indicates that individual susceptibility to UVB radiation needs to be considered when studying the effects of UVB in humans...
  42. ncbi request reprint Seasonal and latitudinal impact of polymorphic light eruption on quality of life
    Tsui C Ling
    J Invest Dermatol 126:1648-51. 2006
  43. ncbi request reprint Induction of nevi and skin tumors in Ink4a/Arf Xpa knockout mice by neonatal, intermittent, or chronic UVB exposures
    Arne van Schanke
    Dermatology Department, University Medical Centre Utrecht, Utrecht, The Netherlands
    Cancer Res 66:2608-15. 2006
    ..Hence, although our experiments did not effectively emulate human melanoma, they provided clear evidence that intermittent UV overexposure strongly stimulates and the Ink4a/Arf(-/-) genotype may actually impair nevus development...
  44. pmc Differential role of basal keratinocytes in UV-induced immunosuppression and skin cancer
    Judith Jans
    MGC, Department of Cell Biology and Genetics, Center for Biomedical Genetics, Erasmus University Medical Center, 3000 DR Rotterdam, The Netherlands
    Mol Cell Biol 26:8515-26. 2006
    ..Taken together, these findings reveal a differential role of basal keratinocytes in these processes, providing novel insights into the skin's acute and chronic responses to UV in a lesion- and cell-type-specific manner...
  45. ncbi request reprint The cell-surface marker MTS24 identifies a novel population of follicular keratinocytes with characteristics of progenitor cells
    Joanne G W Nijhof
    Department of Dermatology, Leiden University Medical Center LUMC The Netherlands
    Development 133:3027-37. 2006
    ..Taken together, these data suggest that the cell-surface marker MTS24 identifies a new reservoir of hair follicle keratinocytes with a proliferative capacity and gene expression profile suggestive of progenitor or stem cells...
  46. ncbi request reprint Epidermal cis-urocanic acid levels correlate with lower specific cellular immune responses after hepatitis B vaccination of ultraviolet B-exposed humans
    Annemarie Sleijffers
    Laboratory for Pathology and Immunobiology, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    Photochem Photobiol 77:271-5. 2003
    ..In other words, volunteers with high cis-UCA levels caused by UVB exposure showed lower cellular immune responses against hepatitis B antigen after hepatitis B vaccination...
  47. ncbi request reprint Climate change and skin cancer
    Jan C van der Leun
    Ecofys, Kanaalweg 16 G, NL 3526 KL Utrecht, The Netherlands
    Photochem Photobiol Sci 1:324-6. 2002
    ..Here we show that it is likely that such an influence will occur. For the same reason, it is likely that the baseline incidence of skin cancer will be augmented by rising temperatures, which may become significant in magnitude...
  48. ncbi request reprint Repair characteristics and differentiation propensity of long-term cultures of epidermal keratinocytes derived from normal and NER-deficient mice
    Claude Backendorf
    Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Einsteinweg 55, 2333 CC Leiden, The Netherlands
    DNA Repair (Amst) 4:1325-36. 2005
    ..It is inferred that these fully controllable in vitro cultures will be a valuable tool to assess critical parameters of genome care-taking systems in cell proliferation and differentiation...
  49. ncbi request reprint Environmental effects of ozone depletion and its interactions with climate change: progress report, 2004
    Anthony Andrady
    Photochem Photobiol Sci 4:177-84. 2005
    ..The complexity of the linkages between ozone depletion, UV-B radiation and climate change has become more apparent...
  50. ncbi request reprint UVB exposure impairs immune responses after hepatitis B vaccination in two different mouse strains
    Annemarie Sleijffers
    National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    Photochem Photobiol 75:541-6. 2002
    ..Susceptibility to UVB-induced immunomodulation depended on the strain of mice and their predilection for developing different T cell responses...