J A Bouwstra

Summary

Affiliation: Leiden University
Country: The Netherlands

Publications

  1. ncbi request reprint Phase behavior of stratum corneum lipid mixtures based on human ceramides: the role of natural and synthetic ceramide 1
    Joke A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands
    J Invest Dermatol 118:606-17. 2002
  2. ncbi request reprint Synthesis and characterization of hyperbranched polyglycerol hydrogels
    Marion H M Oudshoorn
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht University, Sorbonnelaan 16, PO Box 80082, 3508 TB Utrecht, The Netherlands
    Biomaterials 27:5471-9. 2006
  3. ncbi request reprint Water distribution and natural moisturizer factor content in human skin equivalents are regulated by environmental relative humidity
    Joke A Bouwstra
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    J Invest Dermatol 128:378-88. 2008
  4. doi request reprint Human skin equivalents are an excellent tool to study the effect of moisturizers on the water distribution in the stratum corneum
    J A Bouwstra
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    Int J Cosmet Sci 34:560-6. 2012
  5. pmc Skin barrier disruption by acetone: observations in a hairless mouse skin model
    Robert Rissmann
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research LACDR, Leiden University, 2300 RA Leiden, The Netherlands
    Arch Dermatol Res 301:609-13. 2009
  6. ncbi request reprint Water distribution and related morphology in human stratum corneum at different hydration levels
    Joke A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands
    J Invest Dermatol 120:750-8. 2003
  7. ncbi request reprint Structure of the skin barrier and its modulation by vesicular formulations
    Joke A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands
    Prog Lipid Res 42:1-36. 2003
  8. ncbi request reprint New aspects of the skin barrier organization
    J Bouwstra
    Leiden Amsterdam Center for Drug Research, University of Leiden Amsterdam, Leiden, The Netherlands
    Skin Pharmacol Appl Skin Physiol 14:52-62. 2001
  9. ncbi request reprint Phase behavior of lipid mixtures based on human ceramides: coexistence of crystalline and liquid phases
    J A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P O Box 9502, 2300 RA Leiden, The Netherlands
    J Lipid Res 42:1759-70. 2001
  10. ncbi request reprint Phase behaviour of skin barrier model membranes at pH 7.4
    J A Bouwsta
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands
    Cell Mol Biol (Noisy-le-grand) 46:979-92. 2000

Collaborators

  • M Ponec
  • H E Junginger
  • F J Verbaan
  • Wim Jiskoot
  • M J Lawrence
  • G S Pilgram
  • S Pavel
  • Wim E Hennink
  • H Putter
  • G Kersten
  • W Bras
  • Jaap Nijsse
  • F Spies
  • Li Li
  • Threes G M Smijs
  • P Loan Honeywell-Nguyen
  • Gert S Gooris
  • Akhmad Kharis Nugroho
  • Julia Caussin
  • Meindert Danhof
  • Robert Rissmann
  • G S Gooris
  • Ylva Y Grams
  • Suzanne M Bal
  • D Groen
  • Gai Ling Li
  • Marion H M Oudshoorn
  • Miranda W de Jager
  • M Janssens
  • Oliver W Ackaert
  • O W Ackaert
  • Daniel Groen
  • Zhi Ding
  • Z Ding
  • G L Li
  • Durk Dijkstra
  • H W Wouter Groenink
  • M Danhof
  • E H Mojumdar
  • D J Barlow
  • B Deme
  • Anko M de Graaff
  • A M de Graaff
  • Lynne Whitehead
  • Varsha S Thakoersing
  • Jeroen De Graan
  • Johann W Wiechers
  • J Caussin
  • Hendrik W W Groenink
  • D G Fatouros
  • Håkan Wikström
  • Oscar Della-Pasqua
  • T J van Steeg
  • A C van Aelst
  • Dimitrios G Fatouros
  • Paul Cornwell
  • Igor P Dolbnya
  • H K Koerten
  • Arne Grossklaus
  • M W de Jager
  • Peter M Frederik
  • R I Koning
  • J Van Smeden
  • A J Koster
  • R Wolterbeek
  • A A Mulder
  • A P M Lavrijsen
  • B A van den Bergh
  • L A Pechtold
  • J B van Mechelen
  • Jeroen Van Smeden
  • J De Graan
  • H M Wolff
  • J Eikelenboom
  • Elly van Riet
  • Bram Slütter
  • J A Bouwsta
  • B H Westerink
  • Annelieke C Kruithof
  • O E Della Pasqua
  • R Capancioni
  • Romano Capancioni
  • D Dijkstra
  • D J van den Berg
  • Evelien Rozema
  • E van Riet
  • M Bivas-Benita
  • A Huckriede
  • L Bungener
  • S Romeijn

Detail Information

Publications83

  1. ncbi request reprint Phase behavior of stratum corneum lipid mixtures based on human ceramides: the role of natural and synthetic ceramide 1
    Joke A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands
    J Invest Dermatol 118:606-17. 2002
    ..Based on the results of this and previous studies we offer an explanation for the deviation in lipid organization in diseased and in dry skin compared to normal skin...
  2. ncbi request reprint Synthesis and characterization of hyperbranched polyglycerol hydrogels
    Marion H M Oudshoorn
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht University, Sorbonnelaan 16, PO Box 80082, 3508 TB Utrecht, The Netherlands
    Biomaterials 27:5471-9. 2006
    ..Hydrogels based on HyPG have great potential as drug delivery matrices and for tissue engineering purposes...
  3. ncbi request reprint Water distribution and natural moisturizer factor content in human skin equivalents are regulated by environmental relative humidity
    Joke A Bouwstra
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    J Invest Dermatol 128:378-88. 2008
    ..Only topical application of water induced marked swelling of corneocytes. This clearly shows that the stratum corneum water level in HSE is regulated by other, still unknown, factors, in addition to NMF...
  4. doi request reprint Human skin equivalents are an excellent tool to study the effect of moisturizers on the water distribution in the stratum corneum
    J A Bouwstra
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    Int J Cosmet Sci 34:560-6. 2012
    ..Our results show that HSEs can be used as a model to study the water distribution...
  5. pmc Skin barrier disruption by acetone: observations in a hairless mouse skin model
    Robert Rissmann
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research LACDR, Leiden University, 2300 RA Leiden, The Netherlands
    Arch Dermatol Res 301:609-13. 2009
    ..In conclusion, our studies demonstrate that barrier disruption due to acetone treatment is mainly due to removal of corneocytes...
  6. ncbi request reprint Water distribution and related morphology in human stratum corneum at different hydration levels
    Joke A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands
    J Invest Dermatol 120:750-8. 2003
    ..The changes in stratum corneum morphology with increasing water level have also been observed in dermatomed skin...
  7. ncbi request reprint Structure of the skin barrier and its modulation by vesicular formulations
    Joke A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands
    Prog Lipid Res 42:1-36. 2003
    ..In the second part of this paper the possible interactions between vesicles and skin are described, focusing on differences between the effects of gel-state vesicles, liquid-state vesicles and elastic vesicles...
  8. ncbi request reprint New aspects of the skin barrier organization
    J Bouwstra
    Leiden Amsterdam Center for Drug Research, University of Leiden Amsterdam, Leiden, The Netherlands
    Skin Pharmacol Appl Skin Physiol 14:52-62. 2001
    ..From this we conclude that the results obtained with lipid mixtures can be used to predict the SC lipid organization in native tissue...
  9. ncbi request reprint Phase behavior of lipid mixtures based on human ceramides: coexistence of crystalline and liquid phases
    J A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P O Box 9502, 2300 RA Leiden, The Netherlands
    J Lipid Res 42:1759-70. 2001
    ..These changes in phase behavior might be due to a larger amount of linoleic acid moiety in HCER mixtures compared with that in pigCER mixtures...
  10. ncbi request reprint Phase behaviour of skin barrier model membranes at pH 7.4
    J A Bouwsta
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands
    Cell Mol Biol (Noisy-le-grand) 46:979-92. 2000
    ..From our results we can conclude that the lipid mixtures prepared at pH 5 resemble more closely the lipid phase behaviour in intact SC than the lipid mixtures prepared at pH 7.4...
  11. ncbi request reprint The lipid organisation in the skin barrier
    J A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands
    Acta Derm Venereol Suppl (Stockh) 208:23-30. 2000
    ..Finally a new model, the "sandwich model", will be proposed that describe the localisation of the fluid phases in the stratum corneum...
  12. ncbi request reprint The skin barrier in healthy and diseased state
    Joke A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P O Box 9502, 2300 RA Leiden, The Netherlands
    Biochim Biophys Acta 1758:2080-95. 2006
    ..Finally, the information on the role various lipid classes play in lipid phase behavior has been used to interpret the changes in lipid organization and barrier properties of diseased skin...
  13. doi request reprint Improved piercing of microneedle arrays in dermatomed human skin by an impact insertion method
    F J Verbaan
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, P O Box 9502, 2300 RA, Leiden, The Netherlands
    J Control Release 128:80-8. 2008
    ....
  14. doi request reprint The in vitro and in vivo evaluation of new synthesized prodrugs of 5-OH-DPAT for iontophoretic delivery
    O W Ackaert
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, The Netherlands
    J Control Release 144:296-305. 2010
    ..The current findings suggest that beta-alanine-5-OH-DPAT is a promising prodrug, with a good balance between stability, transport efficiency and enzymatic conversion...
  15. ncbi request reprint Interaction of lipophilic moisturizers on stratum corneum lipid domains in vitro and in vivo
    J Caussin
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    Skin Pharmacol Physiol 20:175-86. 2007
    ....
  16. pmc Infrared spectroscopic study of stratum corneum model membranes prepared from human ceramides, cholesterol, and fatty acids
    G S Gooris
    Leiden Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Leiden University, 2300 RA Leiden, The Netherlands
    Biophys J 92:2785-95. 2007
    ..Extrapolating these findings to the situation in stratum corneum led us conclude that in stratum corneum, fatty acids and CER participate in the orthorhombic lattice at 32 degrees C, the skin temperature...
  17. pmc Immune modulation by adjuvants combined with diphtheria toxoid administered topically in BALB/c mice after microneedle array pretreatment
    Z Ding
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research LACDR, Leiden University, Einsteinweg 55, 2333CC Leiden, The Netherlands
    Pharm Res 26:1635-43. 2009
    ..In this study, modulation of the immune response against diphtheria toxoid (DT) by various adjuvants in transcutaneous immunization (TCI) with microneedle array pretreatment was investigated...
  18. doi request reprint Infrared spectroscopy studies of mixtures prepared with synthetic ceramides varying in head group architecture: coexistence of liquid and crystalline phases
    M Janssens
    Leiden Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, Leiden University, P O Box 9502, 2300 RA Leiden, The Netherlands
    Biochim Biophys Acta 1788:732-42. 2009
    ..Furthermore, while the majority of the lipids form a crystalline packing, the linoleate moiety of the acylCERs participates in a "pseudo fluid" phase...
  19. ncbi request reprint The influence of two azones and sebaceous lipids on the lateral organization of lipids isolated from human stratum corneum
    G S Pilgram
    Center for Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands
    Biochim Biophys Acta 1511:244-54. 2001
    ..In mixtures with sebaceous lipids, the presence of the hexagonal lattice increased. These findings provide a basis for the mechanism by which these enhancers and sebaceous lipids interact with human SC lipids...
  20. doi request reprint Electron diffraction study of lipids in non-lesional stratum corneum of atopic eczema patients
    M Janssens
    Department of Drug Delivery Technology, Leiden University, Leiden, The Netherlands
    Biochim Biophys Acta 1828:1814-21. 2013
    ..These results are indicative for the importance of the lipid organization for a proper skin barrier function...
  21. ncbi request reprint Visualization studies of human skin in vitro/in vivo under the influence of an electrical field
    D G Fatouros
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, P O Box 9502 2300 RA, Leiden, The Netherlands
    Eur J Pharm Sci 29:160-70. 2006
    ..As far as structural changes in stratum corneum are concerned iontophoresis is therefore a safe method at the experimental conditions we used...
  22. ncbi request reprint The phase behaviour of skin lipid mixtures based on synthetic ceramides
    M W de Jager
    Leiden Amsterdam Center for Drug Research, Department of Pharmaceutical Technology, University of Leiden, P O Box 9502, 2300 RA, Leiden, The Netherlands
    Chem Phys Lipids 124:123-34. 2003
    ..Only a mixture containing synthetic CER1 and CER3, CHOL and FFA showed similar phase behaviour to that of SC...
  23. ncbi request reprint Cutaneous side-effects of transdermal iontophoresis with and without surfactant pretreatment: a single-blinded, randomized controlled trial
    G L Li
    Department of Pharmaceutical Technology, University of Leiden, Leiden, The Netherlands
    Br J Dermatol 153:404-12. 2005
    ..Specific and strictly controlled studies on the dermal effect of iontophoresis are scarce...
  24. doi request reprint Comparing different salt forms of rotigotine to improve transdermal iontophoretic delivery
    O W Ackaert
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden, The Netherlands
    Eur J Pharm Biopharm 74:304-10. 2010
    ....
  25. pmc Disposition of ceramide in model lipid membranes determined by neutron diffraction
    D Groen
    Leiden Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Leiden, The Netherlands
    Biophys J 100:1481-9. 2011
    ..The scattering length density profile of the 5.4-nm phase containing this deuterated ceramide demonstrated a symmetric arrangement of the ceramides with interdigitating acyl chains in the center of the unit cell...
  26. ncbi request reprint Elasticity of vesicles assessed by electron spin resonance, electron microscopy and extrusion measurements
    B A van den Bergh
    Department of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, University of Leiden, P.O. Box 9502, 2300 RA, Leiden, The Netherlands
    Int J Pharm 217:13-24. 2001
    ..The enhanced elasticity might contribute to an optimal design of vesicles as drug carriers for transdermal application...
  27. pmc Localization of cholesterol and Fatty Acid in a model lipid membrane: a neutron diffraction approach
    E H Mojumdar
    Leiden Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Leiden, The Netherlands
    Biophys J 105:911-8. 2013
    ..The fatty acid headgroups were located at the unit cell boundary with their acyl chains straddling the unit cell center. Based on these results, a molecular model is proposed for the arrangement of the lipids within the unit cell. ..
  28. ncbi request reprint X-ray microanalysis of cryopreserved human skin to study the effect of iontophoresis on percutaneous ion transport
    L A Pechtold
    Leiden Amsterdam Center for Drug Research, Division of Pharmaceutical Technology, The Netherlands
    Pharm Res 18:1012-7. 2001
    ..The presence of iodide inside corneocytes suggests the possibility of transcellular percutaneous iodide transport...
  29. ncbi request reprint Assembled microneedle arrays enhance the transport of compounds varying over a large range of molecular weight across human dermatomed skin
    F J Verbaan
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, 2300 RA, Leiden, The Netherlands
    J Control Release 117:238-45. 2007
    ..However, some degradation was also observed for FITC-Dex and DCB. We conclude that assembled microneedle arrays can be used to deliver compounds through the skin up to a molecular weight of at least 72 kDa...
  30. ncbi request reprint Transdermal iontophoretic delivery of apomorphine in patients improved by surfactant formulation pretreatment
    G L Li
    Department of Pharmaceutical Technology, Leiden Amsterdam Centre for Drug Research, University of Leiden, P O Box 9502, 2300 RA, Leiden, The Netherlands
    J Control Release 101:199-208. 2005
    ..1 nmol/cm2 h) compared to the control patients (iontophoresis without absorption enhancers). In five out of eight patients in the study group and in three out of eight patients in the control group, clinical improvement was observed...
  31. pmc New insights into the stratum corneum lipid organization by X-ray diffraction analysis
    D Groen
    Leiden Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Leiden, The Netherlands
    Biophys J 97:2242-9. 2009
    ..This demonstrates that the lipid mixtures serve as an excellent model for the lipid organization in SC, not only with respect to the repeat distance, but also in terms of the electron density distribution within the unit cell...
  32. ncbi request reprint New insights into ultrastructure, lipid composition and organization of vernix caseosa
    Robert Rissmann
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    J Invest Dermatol 126:1823-33. 2006
    ..The results suggest a lower degree of ordering of VC lipids as compared to the SC. A small-angle X-ray diffraction study showed similar results...
  33. ncbi request reprint Temperature-induced changes in structural and physicochemical properties of vernix caseosa
    Robert Rissmann
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    J Invest Dermatol 128:292-9. 2008
    ..In conclusion, we demonstrated that VC properties adjust to the fundamental change from the intrauterine to the post-natal environment...
  34. ncbi request reprint Interactions between liposomes and human skin in vitro, a confocal laser scanning microscopy study
    M E van Kuijk-Meuwissen
    Division of Pharmaceutical Technology, Leiden Amsterdam Center of Drug Research, Leiden University, P O Box 9502, Leiden 2300RA, The Netherlands
    Biochim Biophys Acta 1371:31-9. 1998
    ..The label applied non-occlusively in flexible liposomes penetrated deeper into the skin than after occlusive application...
  35. ncbi request reprint Aberrant lipid organization in stratum corneum of patients with atopic dermatitis and lamellar ichthyosis
    G S Pilgram
    Center for Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands
    J Invest Dermatol 117:710-7. 2001
    ..Finally, using freeze fracture electron microscopy, changes in the lamellar organization in stratum corneum of both patient groups could be observed...
  36. ncbi request reprint Skin structure and mode of action of vesicles
    J A Bouwstra
    Leiden Amsterdam Center for Drug Research, Leiden University, P O Box 9502, 2300 RA, Leiden, The Netherlands
    Adv Drug Deliv Rev 54:S41-55. 2002
    ..In the second part of this paper, the possible interactions between vesicles and skin are described, focusing on differences between the effects of gel-state, liquid-state, and elastic vesicles...
  37. ncbi request reprint Electron diffraction provides new information on human stratum corneum lipid organization studied in relation to depth and temperature
    G S Pilgram
    Center for Electron Microscopy, Leiden University Medical Center, The Netherlands
    J Invest Dermatol 113:403-9. 1999
    ....
  38. ncbi request reprint Cryo-electron diffraction as a tool to study local variations in the lipid organization of human stratum corneum
    G S Pilgram
    Laboratory for Electron Microscopy, Leiden University Medical Centre, The Netherlands
    J Microsc 189:71-8. 1998
    ..c. by wide-angle X-ray diffraction. After lipid extraction, such diffraction patterns could no longer be detected in the samples...
  39. ncbi request reprint Modelling the stratum corneum lipid organisation with synthetic lipid mixtures: the importance of synthetic ceramide composition
    Miranda W de Jager
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Department of Drug Delivery Technology, University of Leiden, PO Box 9502, 2300 RA, The Netherlands
    Biochim Biophys Acta 1664:132-40. 2004
    ..Furthermore, the formation of the 13-nm lamellar phase is not very sensitive to variations in the total CER level, which is similar to the in vivo situation...
  40. doi request reprint Hydrophilic and lipophilic moisturizers have similar penetration profiles but different effects on SC water distribution in vivo
    Julia Caussin
    Leiden Amsterdam Center for Drug Research, Division of Drug Delivery Technology, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands
    Exp Dermatol 18:954-61. 2009
    ..However, a single application of 3 h is probably too short to exert increased hydration as measured with ATR-FTIR...
  41. doi request reprint Model membranes prepared with ceramide EOS, cholesterol and free fatty acids form a unique lamellar phase
    Daniel Groen
    Division of Drug Delivery Technology, P O Box 9502, 2300 RA, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    Langmuir 26:4168-75. 2010
    ..On the basis of these observations, a molecular model for the 14.7 nm phase has been proposed in which the lipids are organized in a lamellar phase with three different lipid layers in a symmetric unit cell...
  42. doi request reprint Microneedle arrays for the transcutaneous immunization of diphtheria and influenza in BALB/c mice
    Z Ding
    Department of Drug Delivery Technology, Leiden Amsterdam Centre for Drug Research, 2333 CC, Leiden, The Netherlands
    J Control Release 136:71-8. 2009
    ..These results indicate that TCI of DT and CT with microneedle treatment results in comparable protection as injection of DT-alum, and TCI of influenza vaccine adjuvanted with CT is superior to the injection of plain vaccine...
  43. ncbi request reprint Combined chemical and electrical enhancement modulates stratum corneum structure
    A M de Graaff
    Department of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, University of Leiden, P O Box 9502, 2300 RA, Leiden, The Netherlands
    J Control Release 90:49-58. 2003
    ..The observed changes in the ultrastructure of the stratum corneum can explain the increased R-apomorphine transport during iontophoresis...
  44. doi request reprint FTIR studies show lipophilic moisturizers to interact with stratum corneum lipids, rendering the more densely packed
    Julia Caussin
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands
    Biochim Biophys Acta 1778:1517-24. 2008
    ..Especially the thermotropic stabilization of the orthorhombic lateral packing, which might reduce water loss from the skin, is considered to contribute to the moisturizing effect of IPIS and ISIS in stratum corneum...
  45. ncbi request reprint Lipid mixtures prepared with well-defined synthetic ceramides closely mimic the unique stratum corneum lipid phase behavior
    Miranda W de Jager
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    J Lipid Res 46:2649-56. 2005
    ..In conclusion, lipid mixtures prepared with well-defined synthetic ceramides offer an attractive tool with which to unravel the importance of the molecular structure of individual ceramides for proper lipid organization...
  46. doi request reprint Generation of human skin equivalents under submerged conditions-mimicking the in utero environment
    Varsha S Thakoersing
    Department of Drug Delivery Technology, Leiden University, Leiden, The Netherlands
    Tissue Eng Part A 16:1433-41. 2010
    ..These results show that the presented cell culture method can be utilized to generate HSEs under submerged conditions to study epidermal formation under aqueous conditions...
  47. ncbi request reprint Novel lipid mixtures based on synthetic ceramides reproduce the unique stratum corneum lipid organization
    Miranda W de Jager
    Leiden Amsterdam Center for Drug Research, Department of Drug Delivery Technology, University of Leiden, Leiden, The Netherlands
    J Lipid Res 45:923-32. 2004
    ....
  48. ncbi request reprint Lipophilic and hydrophilic moisturizers show different actions on human skin as revealed by cryo scanning electron microscopy
    Julia Caussin
    Gorlaeus Laboratories, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    Exp Dermatol 16:891-8. 2007
    ..Summarizing, SC cells are not equally sensitive to moisturizer application: centrally located corneocytes are more sensitive than corneocytes in the upper and the lowest regions of the SC...
  49. ncbi request reprint Skin penetration and mechanisms of action in the delivery of the D2-agonist rotigotine from surfactant-based elastic vesicle formulations
    P Loan Honeywell-Nguyen
    Department of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, University of Leiden, 2300 RA Leiden, The Netherlands
    Pharm Res 20:1619-25. 2003
    ..This study was performed to investigate the effect of elastic and rigid vesicles on the penetration of the D2 dopamine agonist rotigotine across human skin and to further elucidate the mechanisms of action of the elastic vesicles...
  50. ncbi request reprint Transdermal iontophoresis of rotigotine: influence of concentration, temperature and current density in human skin in vitro
    Akhmad Kharis Nugroho
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, University of Leiden, P O Box 9502, Einsteinweg 55, 2300 RA Leiden, The Netherlands
    J Control Release 96:159-67. 2004
    ..The maximum Flux(ss) achieved was around 80 nmol cm(-2) h(-1) indicating that by means of iontophoresis, a therapeutic level of rotigotine might be achieved with a reasonable patch size...
  51. ncbi request reprint Quantitative assessment of the transport of elastic and rigid vesicle components and a model drug from these vesicle formulations into human skin in vivo
    P Loan Honeywell-Nguyen
    Department of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, University of Leiden, Leiden, The Netherlands
    J Invest Dermatol 123:902-10. 2004
    ..The elastic vesicles are superior to the rigid vesicles both in terms of vesicular transport into the SC and in terms of therapeutic potential as a skin delivery vehicle...
  52. doi request reprint Mechanistic studies of the transdermal iontophoretic delivery of 5-OH-DPAT in vitro
    Oliver W Ackaert
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Einsteinweg 55, 2333 CC Leiden, The Netherlands
    J Pharm Sci 99:275-85. 2010
    ..0 micromol x cm(-2) h(-1) demonstrating the potential of the iontophoretic delivery of this dopamine agonist for the symptomatic treatment of Parkinson's disease...
  53. pmc Microneedle-based transcutaneous immunisation in mice with N-trimethyl chitosan adjuvanted diphtheria toxoid formulations
    Suzanne M Bal
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    Pharm Res 27:1837-47. 2010
    ..The purpose of this study was to gain insight into the delivery and immunogenicity of N-trimethyl chitosan (TMC) adjuvanted diphtheria toxoid (DT) formulations applied transcutaneously with microneedles...
  54. ncbi request reprint The in vitro transport of pergolide from surfactant-based elastic vesicles through human skin: a suggested mechanism of action
    P Loan Honeywell-Nguyen
    Department of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, University of Leiden, P O Box 9502, 2300 RA, Leiden, The Netherlands
    J Control Release 86:145-56. 2003
    ..Based on the results obtained, a mechanism of action for the elastic vesicles was proposed...
  55. ncbi request reprint Pretreatment with a water-based surfactant formulation affects transdermal iontophoretic delivery of R-apomorphine in vitro
    Gai Ling Li
    Department of Pharmaceutical Technology, Leiden Amsterdam Centre for Drug Research, University of Leiden, PO Box 9502, 2300 RA, Leiden, The Netherlands
    Pharm Res 20:653-9. 2003
    ..To further increase the transdermal transport rate of R-apomorphine, a nonocclusive pretreatment with an aqueous surfactant formulation in combination with iontophoresis was explored in vitro...
  56. doi request reprint In vivo assessment of safety of microneedle arrays in human skin
    Suzanne M Bal
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    Eur J Pharm Sci 35:193-202. 2008
    ..This opens the opportunity for dermal and transdermal delivery of drugs and vaccines...
  57. ncbi request reprint The in vivo and in vitro interactions of elastic and rigid vesicles with human skin
    P Loan Honeywell-Nguyen
    Department of Pharmaceutical Technology, Gorlaeus Laboratories, Leiden Amsterdam Center for Drug Research, University of Leiden, P O Box 9502, 2300 RA Leiden, The Netherlands
    Biochim Biophys Acta 1573:130-40. 2002
    ..Elastic vesicles and micelles demonstrated very different interactions with human skin and hence probably also have different mechanisms of action for the enhancement of drug transport...
  58. ncbi request reprint Penetration and distribution of three lipophilic probes in vitro in human skin focusing on the hair follicle
    Ylva Y Grams
    Department of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, Leiden University, P O Box 9502, Einsteinweg 55, 2300 RA, Leiden, The Netherlands
    J Control Release 83:253-62. 2002
    ..However, no conclusion can be drawn about the actual route of transport to the hair follicle...
  59. ncbi request reprint Permeant lipophilicity and vehicle composition influence accumulation of dyes in hair follicles of human skin
    Ylva Y Grams
    Leiden Amsterdam Center for Drug Research, Department of Pharmaceutical Technology, Leiden University, P O Box 9502, Einsteinweg 55, 2300 RA Leiden, The Netherlands
    Eur J Pharm Sci 18:329-36. 2003
    ..Therefore we conclude that targeting to the hair follicle can be increased by the use of lipophilic drugs in combination with surfactant solutions and propylene glycol...
  60. doi request reprint Lipid organization in human and porcine stratum corneum differs widely, while lipid mixtures with porcine ceramides model human stratum corneum lipid organization very closely
    Julia Caussin
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands
    Biochim Biophys Acta 1778:1472-82. 2008
    ..It was found that high concentrations of glycerol change the lamellar organization slightly, while domains with an orthorhombic lateral packing are still observed...
  61. ncbi request reprint Transdermal iontophoresis of the dopamine agonist 5-OH-DPAT in human skin in vitro
    Akhmad Kharis Nugroho
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, University of Leiden, PO Box 9502, 2300 RA Leiden, The Netherlands
    J Control Release 103:393-403. 2005
    ..Thus, based on this in vitro study, transdermal iontophoretic delivery of 5-OH-DPAT is very promising...
  62. ncbi request reprint Compartmental modeling of transdermal iontophoretic transport: I. In vitro model derivation and application
    Akhmad Kharis Nugroho
    Leiden Amsterdam Center for Drug Research, 2300 RA Leiden, The Netherlands
    Pharm Res 21:1974-84. 2004
    ..The objective of this study was to develop a family of compartmental models to describe in a strictly quantitative manner the transdermal iontophoretic transport of drugs in vitro...
  63. ncbi request reprint Elastic vesicles as a tool for dermal and transdermal delivery
    P Loan Honeywell-Nguyen
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, The Netherlands
    J Liposome Res 16:273-80. 2006
    ..In order to increase the number of drugs administered via the transdermal route, novel drug delivery systems have to be designed. Among these systems are iontophoresis, electroporation, microneedles, and vesicular systems...
  64. ncbi request reprint Iontophoretic enhancement of timolol across human dermatomed skin in vitro
    Dimitrios G Fatouros
    Department of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, P O Box RA 9502 2300 Leiden, The Netherlands
    J Drug Target 12:19-24. 2004
    ..Assuming a one to one in vitro/in vivo correlation the Timolol transport in vitro results in therapeutic plasma concentrations in humans with very low current densities limiting possible skin irritation...
  65. ncbi request reprint On-line visualization of dye diffusion in fresh unfixed human skin
    Ylva Y Grams
    Leiden Amsterdam Center for Drug Research, Department of Pharmaceutical Technology, Leiden University, 2300 RA Leiden, The Netherlands
    Pharm Res 21:851-9. 2004
    ..The purpose of the current study was to develop a new method to examine the diffusion in fresh unfixed human skin on-line...
  66. ncbi request reprint Transdermal iontophoresis of rotigotine across human stratum corneum in vitro: influence of pH and NaCl concentration
    Akhmad Kharis Nugroho
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, 2300 RA Leiden, The Netherlands
    Pharm Res 21:844-50. 2004
    ..The aim of this study was to characterize the influence of pH and NaCl concentration on the transdermal iontophoretic transport of the dopamine receptor agonist rotigotine across human stratum corneum (HSC)...
  67. doi request reprint Lanolin-derived lipid mixtures mimic closely the lipid composition and organization of vernix caseosa lipids
    Robert Rissmann
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, P O Box 9502, 2300 RA Leiden, The Netherlands
    Biochim Biophys Acta 1778:2350-60. 2008
    ..The optimal lipid mixture was evaluated on tape-stripped hairless mouse skin in vivo. The rate of barrier recovery was increased and comparable to VC lipid treatment...
  68. ncbi request reprint The in vivo transport of elastic vesicles into human skin: effects of occlusion, volume and duration of application
    P Loan Honeywell-Nguyen
    Department of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, University of Leiden, PO Box 9502, 2300 RA Leiden, The Netherlands
    J Control Release 90:243-55. 2003
    ..Furthermore, we have proposed a hypothesis that the channel-like regions represent imperfections within the intercellular lipid lamellae in areas with highly undulating cornified envelopes...
  69. ncbi request reprint A new method to determine the distribution of a fluorophore in scalp skin with focus on hair follicles
    Ylva Y Grams
    Leiden Amsterdam Center for Drug Research, Department of Pharmaceutical Technology, Leiden University, The Netherlands
    Pharm Res 19:350-4. 2002
  70. ncbi request reprint Elastic vesicles: interaction with human skin and drug transport
    Joke A Bouwstra
    Leiden Amsterdam Center for Drug Research, Gorlaeus Laboratories, Einsteinweg 55, 2300 RA, Leiden, The Netherlands
    Cell Mol Biol Lett 7:222-3. 2002
  71. doi request reprint Transdermal iontophoretic delivery of a novel series of dopamine agonists in vitro: physicochemical considerations
    Oliver W Ackaert
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden, The Netherlands
    J Pharm Pharmacol 62:709-20. 2010
    ..The transdermal iontophoretic delivery of a novel series of 2- aminotetralins and chromanamine-based dopamine agonists was investigated in vitro...
  72. doi request reprint Preparation and characterization of diphtheria toxoid-loaded elastic vesicles for transcutaneous immunization
    Zhi Ding
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    J Drug Target 16:555-63. 2008
    ..DT-vesicles formulated at pH 4.5 were stable and showed high association of DT with preserved antigenicity, and are therefore excellent candidates for future in vivo studies...
  73. ncbi request reprint Iontophoretic R-apomorphine delivery in combination with surfactant pretreatment: in vitro validation studies
    Gai Ling Li
    Department of Pharmaceutical Technology, Leiden Amsterdam Centre for Drug Research, University of Leiden, P O Box 9502, 2300 RA, Leiden, The Netherlands
    Int J Pharm 266:61-8. 2003
    ..A linear relationship between current density and R-apomorphine flux indicates that the iontophoretic delivery combined with surfactant pretreatment allows a controlled and individualised administration of R-apomorphine...
  74. ncbi request reprint Compartmental modeling of transdermal iontophoretic transport II: in vivo model derivation and application
    Akhmad Kharis Nugroho
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, 2300 RA Leiden, The Netherlands
    Pharm Res 22:335-46. 2005
    ..The new models are based on previously proposed compartmental models for the transport in vitro...
  75. ncbi request reprint In vitro iontophoresis of R-apomorphine across human stratum corneum. Structure-transport relationship of penetration enhancement
    Gai Ling Li
    Division of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, Leiden University, P O Box 9502, The Netherlands
    J Control Release 84:49-57. 2002
    ..Moreover, comparison of transport data through shed snake skin with human stratum corneum indicates that both shunt- and intercellular pathways are involved in the iontophoretic transport of R-apomorphine...
  76. ncbi request reprint Time and depth resolved visualisation of the diffusion of a lipophilic dye into the hair follicle of fresh unfixed human scalp skin
    Ylva Y Grams
    Leiden Amsterdam Centre for Drug Research, Department of Pharmaceutical Technology, Leiden University, P O Box 9502, Einsteinweg 55, 2300 RA Leiden, The Netherlands
    J Control Release 98:367-78. 2004
    ..This information can be used to determine penetration pathways in the skin...
  77. doi request reprint Two new methods for preparing a unique stratum corneum substitute
    Daniel Groen
    Leiden Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, P O Box 9502, 2300 RA Leiden, The Netherlands
    Biochim Biophys Acta 1778:2421-9. 2008
    ..In conclusion, the linomat method was chosen as the preferred method for preparing the substitute...
  78. doi request reprint Morphological changes of the dermatophyte Trichophyton rubrum after photodynamic treatment: a scanning electron microscopy study
    Threes G M Smijs
    Leiden University Medical Centre, Leiden, The Netherlands
    Med Mycol 46:315-25. 2008
    ..In case of a non-lethal PDT, fungal re-growth started on the remnants of the treated mycelium...
  79. doi request reprint Efficient induction of immune responses through intradermal vaccination with N-trimethyl chitosan containing antigen formulations
    Suzanne M Bal
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, The Netherlands
    J Control Release 142:374-83. 2010
    ..This demonstrates that TMC functions as an immune potentiator for antigens delivered via the skin...
  80. ncbi request reprint Transdermal delivery of pergolide from surfactant-based elastic and rigid vesicles: characterization and in vitro transport studies
    P Loan Honeywell-Nguyen
    Department of Pharmaceutical Technology, Leiden Amsterdam Center for Drug Research, University of Leiden, The Netherlands
    Pharm Res 19:991-7. 2002
    ..The aim of this study was to investigate the effect of elastic and rigid vesicles on the penetration of pergolide across human skin...
  81. ncbi request reprint Pharmacokinetics and pharmacodynamics analysis of transdermal iontophoresis of 5-OH-DPAT in rats: in vitro-in vivo correlation
    Akhmad Kharis Nugroho
    Division of Drug Delivery Technology Leiden Amsterdam Center for Drug Research, Einsteinweg 55 2300 RA Leiden, The Netherlands
    J Pharm Sci 95:1570-85. 2006
    ..A considerable dopaminergic effect was achieved, indicating the feasibility to reach therapeutically effective concentrations of 5-OH-DPAT upon transdermal iontophoresis...
  82. ncbi request reprint A novel ex vivo skin model to study the susceptibility of the dermatophyte Trichophyton rubrum to photodynamic treatment in different growth phases
    Threes G M Smijs
    Leiden University Medical Centre, Skin Research Laboratory, PO Box 9600, 2300 RC Leiden, The Netherlands
    J Antimicrob Chemother 59:433-40. 2007
    ..rubrum when using red light...
  83. ncbi request reprint Does the single gel phase exist in stratum corneum?
    Joke A Bouwstra
    J Invest Dermatol 118:897-8; author reply 899-901. 2002