Jan W Gratama

Summary

Country: The Netherlands

Publications

  1. ncbi Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Biol Regul Homeost Agents 15:14-22. 2001
  2. ncbi Restoration of expression of signal-transduction molecules in lymphocytes from patients with metastatic renal cell cancer after combination immunotherapy
    J W Gratama
    Department of Medical and Tumor Immunology, University Hospital Rotterdam Daniel den Hoed Cancer Center, The Netherlands
    Cancer Immunol Immunother 48:263-9. 1999
  3. ncbi Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes
    Jan W Gratama
    Laboratory for Clinical and Tumor Immunology, Department of Internal Oncology, Erasmus Medical Center Daniel den Hoed Cancer Center, Groene Hilledijk 301, 3075 EA Rotterdam, The Netherlands
    Cytometry 50:92-101. 2002
  4. ncbi Tetramer-based quantification of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in T-cell-depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection
    J W Gratama
    Department of Clinical and Tumor Immunology, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands
    Blood 98:1358-64. 2001
  5. ncbi Loss of CD34(+) hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erythrocyte lysing reagents
    J W Gratama
    Department of Clinical and Tumor Immunology, University Hospital and Daniel den Hoed Cancer Center, P O Box 5201, 3008 AE, Rotterdam, The Netherlands
    J Immunol Methods 239:13-23. 2000
  6. ncbi Comparison of single and dual-platform assay formats for CD34+ haematopoietic progenitor cell enumeration
    J W Gratama
    Department of Clinical and Tumor Immunology, University Hospital Rotterdam, The Netherlands
    Clin Lab Haematol 21:337-46. 1999
  7. ncbi Flow cytometric quantitation of immunofluorescence intensity: problems and perspectives. European Working Group on Clinical Cell Analysis
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Kliniek, Rotterdam, The Netherlands
    Cytometry 33:166-78. 1998
  8. ncbi Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Kliniek, Rotterdam, The Netherlands
    Cytometry 34:128-42. 1998
  9. ncbi Analysis of factors contributing to the formation of mononuclear cell aggregates ("escapees") in flow cytometric immunophenotyping
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cytometry 29:250-60. 1997
  10. ncbi Diagnostic potential of tetramer-based monitoring of cytomegalovirus-specific CD8+ T lymphocytes in allogeneic stem cell transplantation
    J W Gratama
    Laboratory for Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC Daniel den Hoed, 3008 AE Rotterdam, The Netherlands
    Clin Immunol 106:29-35. 2003

Detail Information

Publications76

  1. ncbi Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Biol Regul Homeost Agents 15:14-22. 2001
    ..Thus, the lower threshold of a safe HPC graft in terms of short- and long-term hematopoiesis may be more accurately defined...
  2. ncbi Restoration of expression of signal-transduction molecules in lymphocytes from patients with metastatic renal cell cancer after combination immunotherapy
    J W Gratama
    Department of Medical and Tumor Immunology, University Hospital Rotterdam Daniel den Hoed Cancer Center, The Netherlands
    Cancer Immunol Immunother 48:263-9. 1999
    ....
  3. ncbi Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes
    Jan W Gratama
    Laboratory for Clinical and Tumor Immunology, Department of Internal Oncology, Erasmus Medical Center Daniel den Hoed Cancer Center, Groene Hilledijk 301, 3075 EA Rotterdam, The Netherlands
    Cytometry 50:92-101. 2002
    ....
  4. ncbi Tetramer-based quantification of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in T-cell-depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection
    J W Gratama
    Department of Clinical and Tumor Immunology, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands
    Blood 98:1358-64. 2001
    ..002). Thus, enumeration of HLA-restricted, CMV-specific CD8+ T cells in the grafts and monitoring of these T cells after SCT may constitute a rapid and sensitive tool to identify SCT recipients at risk for developing CMV disease...
  5. ncbi Loss of CD34(+) hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erythrocyte lysing reagents
    J W Gratama
    Department of Clinical and Tumor Immunology, University Hospital and Daniel den Hoed Cancer Center, P O Box 5201, 3008 AE, Rotterdam, The Netherlands
    J Immunol Methods 239:13-23. 2000
    ..We conclude that all seven lysing reagents can be used with confidence in a lyse-no-wash technique, but that only fixative-free lysing reagents should be used when a washing step is considered necessary...
  6. ncbi Comparison of single and dual-platform assay formats for CD34+ haematopoietic progenitor cell enumeration
    J W Gratama
    Department of Clinical and Tumor Immunology, University Hospital Rotterdam, The Netherlands
    Clin Lab Haematol 21:337-46. 1999
    ..Thus, these results suggest that single-platform methodology can reduce the variation in absolute CD34+ cell numbers between laboratories...
  7. ncbi Flow cytometric quantitation of immunofluorescence intensity: problems and perspectives. European Working Group on Clinical Cell Analysis
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Kliniek, Rotterdam, The Netherlands
    Cytometry 33:166-78. 1998
    ..We identify several issues on the level of instrumentation, reagents, and cells under study that should be solved to allow standardization of quantitative assessments of immunofluorescence intensity...
  8. ncbi Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Kliniek, Rotterdam, The Netherlands
    Cytometry 34:128-42. 1998
    ..An important clinical question to be addressed is the definition of the precise phenotypes and required numbers of HPCs responsible for short- and long-term recovery to optimize HPC transplant strategies...
  9. ncbi Analysis of factors contributing to the formation of mononuclear cell aggregates ("escapees") in flow cytometric immunophenotyping
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cytometry 29:250-60. 1997
    ..These data document the role of mIgG1 mAb binding by human class IIa IgG receptors in the formation of cell aggregates and show that inhibition of this interaction reduces this technical problem in flow cytometric immunophenotyping...
  10. ncbi Diagnostic potential of tetramer-based monitoring of cytomegalovirus-specific CD8+ T lymphocytes in allogeneic stem cell transplantation
    J W Gratama
    Laboratory for Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC Daniel den Hoed, 3008 AE Rotterdam, The Netherlands
    Clin Immunol 106:29-35. 2003
    ..The results thus far warrant the further development of tetramer technology as a diagnostic tool to monitor CMV-specific T cells in SCT recipients and other groups of immunocompromised patients threatened by CMV...
  11. ncbi Reduction of interlaboratory variability in flow cytometric immunophenotyping by standardization of instrument set-up and calibration, and standard list mode data analysis
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cytometry 30:10-22. 1997
    ....
  12. ncbi Analysis of variation in results of CD34+ hematopoietic progenitor cell enumeration in a multicenter study
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cytometry 30:109-17. 1997
    ..2% and 3.9% and CVs ranging between 34% and 106%. We conclude that correct application of this standard protocol was effective in reducing the interlaboratory variation in percentage of CD34+ cells assessments...
  13. ncbi Analysis of variation in results of flow cytometric lymphocyte immunophenotyping in a multicenter study
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cytometry 30:166-77. 1997
    ..We suggest that training and experience in a universally applicable standard protocol are critical for minimization of interlaboratory variation in flow cytometric immunophenotyping...
  14. ncbi Flow cytometric lymphocyte subset enumeration: 10 years of external quality assessment in the Benelux countries
    Wilfried H B M Levering
    Laboratory for Histocompatibility and Immunogenetics, Sanquin Blood Bank South West Region, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 74:79-90. 2008
    ..Failure to fully standardize protocols may have led to only modest reductions in variability of results between laboratories...
  15. doi Long-term stabilized blood samples as controls for flow cytometric HLA-B27 screening: a feasibility study
    Wilfried H B M Levering
    Laboratory for Histocompatibility and Immunogenetics, Sanquin Blood Bank South West Region, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 74:169-81. 2008
    ..We evaluated long-term stabilized whole blood specimens as prepared for the UK NEQAS for Leucocyte Immunophenotyping EQA scheme (Sheffield, UK)...
  16. ncbi Monitoring cytomegalovirus IE-1 and pp65-specific CD4+ and CD8+ T-cell responses after allogeneic stem cell transplantation may identify patients at risk for recurrent CMV reactivations
    Jan W Gratama
    Department of Internal Oncology, Erasmus MC, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 74:211-20. 2008
    ..Thus, monitoring of particular CMV-specific CD4+ and CD8+ T-cell responses after allogeneic SCT may identify patients at risk for recurrent CMV reactivations...
  17. pmc No evidence for circulating HuD-specific CD8+ T cells in patients with paraneoplastic neurological syndromes and Hu antibodies
    Janet W de Beukelaar
    Department of Medical Oncology, Room E 2 80B, Erasmus University Medical Center, Groene Hilledijk 301, 3075 EA, Rotterdam, The Netherlands
    Cancer Immunol Immunother 56:1501-6. 2007
    ..To gain more insight into the hypothesized CD8(+ )T cell-mediated immune pathogenesis of these syndromes, we searched for circulating HuD-specific CD8(+) T cells in a large cohort of Hu-PNS patients and controls...
  18. doi Effector memory and late memory T cells accumulate in the blood of CMV-carrying individuals but not in their cerebrospinal fluid
    Adriaan H C de Jongste
    Department of Medical Oncology, Erasmus University Medical Centre Daniel den Hoed Cancer Centre, Groene Hilledijk 301, 3075EA, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 84:218-21. 2013
    ..In conclusion, there was no demonstrable difference in the numbers of CSF effector memory and late memory T cells between CMV seronegative and CMV seropositive individuals...
  19. doi Contamination of synthetic HuD protein spanning peptide pools with a CMV-encoded peptide
    Marieke T de Graaf
    Department of Neurology, Erasmus University Medical Center, Rotterdam, The Netherlands
    Cytometry A 73:1079-85. 2008
    ..Our data reveal that synthetic PSPP may contain immunogenic contaminations which may cause false positive results in T-cell stimulation assays...
  20. doi Immune monitoring with iTAg MHC Tetramers for prediction of recurrent or persistent cytomegalovirus infection or disease in allogeneic hematopoietic stem cell transplant recipients: a prospective multicenter study
    Jan W Gratama
    Department of Clinical and Tumor Immunology, Erasmus Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Blood 116:1655-62. 2010
    ..004). CMV tetramer-based immune monitoring, in conjunction with virologic monitoring, can be an important new tool to assess risk of CMV-related complications and to guide preemptive therapeutic choices...
  21. doi Flow cytometric screening for the HLA-B27 antigen on peripheral blood lymphocytes
    Wilfried H B M Levering
    Laboratory for Histocompatibility and Immunogenetics, Sanquin Blood, Bank South West Region, Rotterdam, The Netherlands
    Curr Protoc Cytom . 2005
    ..Lyse-and-wash sample processing is employed. A multiparameter flow methodology is applied. Three to four parameters-forward scatter, side scatter, HLA-B27 expression, and, in some cases CD3 or B7 expression-are acquired...
  22. ncbi Flow cytometric CD34+ stem cell enumeration: lessons from nine years' external quality assessment within the Benelux countries
    Wilfried H B M Levering
    Laboratory for Histocompatibility and Immunogenetics, Sanquin Blood Bank South West Region, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 72:178-88. 2007
    ..In an evaluation of the results of 16 send-outs, we studied the effects of the methods used on assay outcome and whether or not this exercise was effective in reducing between-laboratory variation...
  23. doi Clinical value of circulating endothelial cell detection in oncology
    Jaco Kraan
    Department of Medical Oncology, Erasmus Medical Center Daniel den Hoed Cancer Center, Groene Hilledijk 301, 3075 EA Rotterdam, The Netherlands
    Drug Discov Today 17:710-7. 2012
    ..Here, we address the current state-of-the-art of CEC enumeration as a biomarker in clinical oncology. We focus on technical issues concerning CEC detection, review results from clinical studies and explore future potential applications...
  24. ncbi Flow cytometric characterization of cerebrospinal fluid cells
    Marieke T de Graaf
    Department of Neurology, Erasmus University Medical Center, s Gravendijkwal 230, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 80:271-81. 2011
    ....
  25. doi Analytical performance of a standardized single-platform MHC tetramer assay for the identification and enumeration of CMV-specific CD8+ T lymphocytes
    Rik A Brooimans
    Department of Internal Oncology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cytometry A 73:992-1000. 2008
    ..0-10.0 cells/microL)...
  26. ncbi Correlation between circulating endothelial cell counts and plasma thrombomodulin levels as markers for endothelial damage
    Michiel H Strijbos
    Erasmus University Medical Center Daniel den Hoed Cancer Center, Groene Hilledijk 301, Rotterdam, The Netherlands
    Thromb Haemost 100:642-7. 2008
    ..Higher levels of thrombomodulin and E-selectin were observed in males when compared to females. In conclusion, CEC numbers correlate positively with plasma levels of thrombomodulin...
  27. doi Treatment of metastatic renal cell carcinoma with CAIX CAR-engineered T cells: clinical evaluation and management of on-target toxicity
    Cor Hj Lamers
    Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Mol Ther 21:904-12. 2013
    ..We provide in-patient proof that the observed "on-target" toxicity is antigen-directed and can be prevented by blocking antigenic sites in off-tumor organs and allowing higher T cell doses...
  28. ncbi T-lymphocyte reconstitution following rigorously T-cell-depleted versus unmodified autologous stem cell transplants
    P A W te Boekhorst
    Department of Hematology, Erasmus Medical Center, Rotterdam, The Netherlands
    Bone Marrow Transplant 37:763-72. 2006
    ..This susceptibility to apoptosis would interfere with a swift and sustained CD4(+) T-cell regeneration post SCT...
  29. doi Elevated numbers of regulatory T cells, central memory T cells and class-switched B cells in cerebrospinal fluid of patients with anti-Hu antibody associated paraneoplastic neurological syndromes
    Adriaan H C de Jongste
    Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Neuroimmunol 258:85-90. 2013
    ..These findings support the hypothesis that the immune system is locally activated in Hu-PNS, and suggests common etiological pathways between Hu-PNS and other inflammatory central nervous system disorders...
  30. doi Levels of circulating endothelial cells in normotensive and severe preeclamptic pregnancies
    Michiel H Strijbos
    Department of Medical Oncology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 78:382-6. 2010
    ....
  31. doi External quality assurance of circulating tumor cell enumeration using the CellSearch(®) system: a feasibility study
    Jaco Kraan
    Department of Medical Oncology, Erasmus Medical Center, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 80:112-8. 2011
    ..We studied the feasibility of external quality assurance (EQA) of the entire CellSearch procedure from blood draw to interpretation of results in multiple laboratories...
  32. doi CD49f-based selection of circulating tumor cells (CTCs) improves detection across breast cancer subtypes
    Bianca Mostert
    Erasmus MC, Department of Medical Oncology, Daniel den Hoed Cancer Center and Laboratory of Translational Tumor Immunology, Groene Hilledijk 301, 3075 EA Rotterdam, The Netherlands
    Cancer Lett 319:49-55. 2012
    ..Combined staining of CK8/18/19 and CD49f after CD146/EpCAM enrichment is likely to further improve CTC detection in breast cancer...
  33. ncbi On the origin of (CD105+) circulating endothelial cells
    Michiel H Strijbos
    Erasmus University Medical Center Daniel den Hoed Cancer, Center Groene Hilledijk 301, 3075 EA Rotterdam, The Netherlands
    Thromb Haemost 102:347-51. 2009
    ....
  34. doi HLA-DQ2+ individuals are susceptible to Hu-Ab associated paraneoplastic neurological syndromes
    Marieke T de Graaf
    Department of Neurology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Neuroimmunol 226:147-9. 2010
    ..Hypothetically, T cells are involved in the pathogenesis of paraneoplastic neurological syndromes associated with Hu-antibodies (Hu-PNS)...
  35. ncbi Resolution of EBV(+) CNS lymphoma with appearance of CSF EBV-specific T cells
    Janet W de Beukelaar
    Department of Medical Oncology, Erasmus MC, Rotterdam, The Netherlands
    Ann Neurol 58:788-92. 2005
    ..The appearance of EBV-specific CD8(+) T cells in CSF and blood correlated with neurological improvement and disappearance of EBV-LPD. These observations suggest a role for EBV-specific CD8(+) T cells in the control of EBV-LPD in the CNS...
  36. ncbi Parallel detection of transduced T lymphocytes after immunogene therapy of renal cell cancer by flow cytometry and real-time polymerase chain reaction: implications for loss of transgene expression
    C H J Lamers
    Laboratory of Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC Daniel den Hoed Cancer Center, 3008 AE Rotterdam, The Netherlands
    Hum Gene Ther 16:1452-62. 2005
    ..These results suggest loss of scFv(G250) membrane expression on adoptive transfer, which would have important implications for the antitumor efficacy of this form of immunogene therapy...
  37. ncbi Imbalances in circulating lymphocyte subsets in Hu antibody associated paraneoplastic neurological syndromes
    J W de Beukelaar
    Department of Medical Oncology, Erasmus University Medical Center, Rotterdam, The Netherlands
    Eur J Neurol 14:1383-91. 2007
    ..Although indirect, our data provide further support for the involvement of T cells in the pathogenesis of Hu antibody associated PNS...
  38. ncbi Impaired recovery of Epstein-Barr virus (EBV)--specific CD8+ T lymphocytes after partially T-depleted allogeneic stem cell transplantation may identify patients at very high risk for progressive EBV reactivation and lymphoproliferative disease
    Pauline Meij
    Department of Internal Oncology, Erasmus MC, Rotterdam, The Netherlands
    Blood 101:4290-7. 2003
    ....
  39. doi Flow cytometric immunophenotyping of cerebrospinal fluid
    Jaco Kraan
    Department of Medical Oncology, Erasmus Medical Center, Rotterdam, The Netherlands
    Curr Protoc Cytom . 2008
    ..Finally, in the Alternate Protocol, we describe a method to calculate absolute numbers of both normal and pathological cell subpopulations by adding counting beads to the assay...
  40. doi Circulating endothelial cells: a potential parameter of organ damage in sickle cell anemia?
    Michiel H Strijbos
    Department of Medical Oncology, Erasmus Medical Center, Rotterdam, The Netherlands
    Blood Cells Mol Dis 43:63-7. 2009
    ..002). No significant correlations between CEC and any other laboratory parameter were detected. In conclusion, CECs could prove to be an important new tool for assessing developing vasculopathy and organ damage in SCD...
  41. doi Immune responses to transgene and retroviral vector in patients treated with ex vivo-engineered T cells
    Cor H J Lamers
    Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Blood 117:72-82. 2011
    ....
  42. ncbi The impact of impurities in synthetic peptides on the outcome of T-cell stimulation assays
    Janet W de Beukelaar
    Laboratory of Neuro Oncology, Department of Neurology, Erasmus University Medical Center, Rotterdam, The Netherlands
    Rapid Commun Mass Spectrom 21:1282-8. 2007
    ..Our data reveal that different batches from the same peptide may contain artefacts that influence the outcome of HLA-restricted T-cell response assays...
  43. ncbi Flow cytometric HLA-B27 screening: cross-reactivity patterns of commercially available anti-HLA-B27 monoclonal antibodies with other HLA-B antigens
    Wilfried H B M Levering
    Laboratory for Histocompatibility and Immunogenetics, Sanquin Blood Bank South West Region, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 54:28-38. 2003
    ..Results from this scheme indicate that cross-reactivity of HLA-B27 monoclonal antibodies (mAbs) is a major problem...
  44. ncbi CD38 as a prognostic factor in B cell chronic lymphocytic leukaemia (B-CLL): comparison of three approaches to analyze its expression
    Joke G Boonstra
    Department of Clinical Chemistry, University Medical Center Rotterdam, Daniel den Hoed Cancer Center, The Netherlands
    Cytometry B Clin Cytom 70:136-41. 2006
    ..Increased CD38 expression by leukemic cells has been suggested as an adverse prognostic factor in B-CLL. Several approaches have been proposed to quantify its level of expression by flow cytometry...
  45. ncbi Use of TransFix™ cerebrospinal fluid storage tubes prevents cellular loss and enhances flow cytometric detection of malignant hematological cells after 18 hours of storage
    Adriaan H de Jongste
    Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Groene Hilledijk 301, 3075 EA Rotterdam, The Netherlands Department of Neurology, Erasmus University Medical Center, Rotterdam s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Cytometry B Clin Cytom 86:272-9. 2014
    ..We conclude that the use of TransFix-containing CSF storage tubes prevents cellular loss and enhances flow cytometric detection of LHM after 18 hours of storage. © 2013 International Clinical Cytometry Society. ..
  46. ncbi Repeated administrations of interleukin (IL)-12 are associated with persistently elevated plasma levels of IL-10 and declining IFN-gamma, tumor necrosis factor-alpha, IL-6, and IL-8 responses
    Johanna E A Portielje
    Department of Medical Oncology, Erasmus MC Daniel den Hoed, 3075 EA Rotterdam, The Netherlands
    Clin Cancer Res 9:76-83. 2003
    ..Induction of IFN-gamma, considered a key mediator of antitumor effects of IL-12, is known to decline on repeated administrations. We studied whether other immunological effects of rHuIL-12 are tapered in the course of treatment...
  47. ncbi Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells
    J W Gratama
    Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Semin Hematol 38:139-47. 2001
    ..Using such a standardized assay, it should be possible to define more accurately the lower threshold for a safe HPC graft in terms of short- and long-term hematopoietic reconstitution. Semin Hematol 38:139-147...
  48. pmc Interleukin 2 and interferon alpha-2a do not improve anti-tumour activity of 5-fluorouracil in advanced colorectal cancer
    S H Goey
    Department of Medical Oncology, Rotterdam Cancer Institute Daniel den Hoed Kliniek, The Netherlands
    Br J Cancer 74:2018-23. 1996
    ..This regimen of IL-2 and IFN alpha-2a with 5-FU has only modest anti-tumour activity in advanced colorectal cancer...
  49. ncbi Setting up and calibration of a flow cytometer for multicolor immunophenotyping
    J Kraan
    Department of Internal Oncology, Erasmus MC Daniel den Hoed Cancer Centre, Rotterdam, The Netherlands
    J Biol Regul Homeost Agents 17:223-33. 2003
  50. ncbi Flow cytometric screening for HLA-B27 on peripheral blood lymphocytes
    W H B M Levering
    Laboratory for Histocompatibility and Immunogenetics, Sanquin Bloodbank South West Region, Rotterdam, The Netherlands
    J Biol Regul Homeost Agents 17:241-6. 2003
  51. ncbi Adoptive immuno-gene therapy of cancer with single chain antibody [scFv(Ig)] gene modified T lymphocytes
    C H J Lamers
    Department of Medical Oncology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Biol Regul Homeost Agents 18:134-40. 2004
    ..Currently, we evaluate this concept in a clinical protocol to treat patients with metastatic renal cell cancer (RCC) using autologous RCC-specific gene-modified T lymphocytes...
  52. ncbi Clinical utility of tetramer-based immune monitoring in allogeneic stem cell transplantation
    Jan W Gratama
    Laboratory for Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC Daniel den Hoed, Rotterdam, The Netherlands
    BioDrugs 17:325-38. 2003
    ....
  53. doi mRNA and microRNA expression profiles in circulating tumor cells and primary tumors of metastatic breast cancer patients
    Anieta M Sieuwerts
    Department of Medical Oncology, Josephine Nefkens Institute and Cancer Genomics Centre, Rotterdam, The Netherlands
    Clin Cancer Res 17:3600-18. 2011
    ..In this study, we determined mRNA and microRNA (miRNA) expression of potentially CTC-specific genes that are considered to be clinically relevant in breast cancer...
  54. ncbi Cells meeting our immunophenotypic criteria of endothelial cells are large platelets
    Michiel H Strijbos
    Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Groene Hilledijk 301, 3075 EA Rotterdam, The Netherlands
    Cytometry B Clin Cytom 72:86-93. 2007
    ..Blood 2001;97:3658-3661). Here, we set out to confirm the endothelial nature of these cells...
  55. ncbi Validation of the single-platform ISHAGE method for CD34(+) hematopoietic stem and progenitor cell enumeration in an international multicenter study
    J W Gratama
    Department of Internal Oncology, Erasmus Medical Center Rotterdam, The Netherlands
    Cytotherapy 5:55-65. 2003
    ..e., multiparametric definition of HPC based on light scatter, expression of CD34 and CD45, and counting beads (i.e., 'single platform ISHAGE' method)...
  56. pmc Circulating endothelial cells in oncology: pitfalls and promises
    M H Strijbos
    Department of Medical Oncology, Erasmus University Medical Center, Rotterdam, The Netherlands
    Br J Cancer 98:1731-5. 2008
    ..In this review, we will address the possible clinical applications of CEC detection in oncology, as well as the pitfalls encountered in this process...
  57. ncbi Flow cytometric enumeration of Class I HLA-restricted, peptide-specific CD8+ T lymphocytes using tetramer technology and single-platform absolute T-cell counting
    J Kraan
    Department of Internal Oncology, Erasmus MC, Daniel den Hoed Cancer Centre, Rotterdam, The Netherlands
    J Biol Regul Homeost Agents 17:268-78. 2003
  58. ncbi Flow cytometric enumeration of antigen-specific T lymphocytes
    J W Gratama
    Laboratory for Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC Daniel den Hoed, Rotterdam, The Netherlands
    Cytometry A 58:79-86. 2004
  59. ncbi Visual scoring versus histogram subtraction of in vivo binding of immunoglobulins against platelets after transfusion
    M D Levin
    Department of Hematology, Erasmus Medical Center, Daniel den Hoed, Rotterdam, The Netherlands
    Cytometry B Clin Cytom 57:40-6. 2004
    ..The visually scored results of this technique, however, are susceptible to interobserver variation. We describe a more objective method to generate results in IVBI-PIFT...
  60. ncbi Process validation and clinical evaluation of a protocol to generate gene-modified T lymphocytes for imunogene therapy for metastatic renal cell carcinoma: GMP-controlled transduction and expansion of patient's T lymphocytes using a carboxy anhydrase IX-s
    C H J Lamers
    Unit Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cytotherapy 8:542-53. 2006
    ..We describe the validation of our clinical protocol for gene transduction and expansion of human T lymphocytes...
  61. pmc Intrapleural administration of interleukin 2 in pleural mesothelioma: a phase I-II study
    S H Goey
    Department of Medical Oncology, Rotterdam Cancer Institute Daniel den Hoed Kliniek, The Netherlands
    Br J Cancer 72:1283-8. 1995
    ....
  62. pmc Dose efficacy study of two schedules of high-dose bolus administration of interleukin 2 and interferon alpha in metastatic melanoma
    W H Kruit
    Department of Medical Oncology, Rotterdam Cancer Institute Daniel den Hoed Kliniek, The Netherlands
    Br J Cancer 74:951-5. 1996
    ..The 3 day schedule was associated with manageable toxicity, but yielded a moderate response rate of 20% (CI 7-43%)...
  63. ncbi The use of CD38 expression by monoclonal B lymphocytes as a prognostic factor in B-cell chronic lymphocytic leukemia
    J G Boonstra
    Department of Clinical Chemistry, University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Biol Regul Homeost Agents 18:340-6. 2004
    ..CD38 expression may be analyzed as percentage positive cells or as antibodies bound per cell. Addition of CD38 to the flow cytometry antibody panel for B-CLL analysis is a relatively easy way to obtain important prognostic information...
  64. ncbi CSF flow cytometry greatly improves diagnostic accuracy in CNS hematologic malignancies
    J E C Bromberg
    Department of Neuro Oncology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Neurology 68:1674-9. 2007
    ....
  65. ncbi Phoenix-ampho outperforms PG13 as retroviral packaging cells to transduce human T cells with tumor-specific receptors: implications for clinical immunogene therapy of cancer
    C H J Lamers
    Unit of Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cancer Gene Ther 13:503-9. 2006
    ..The improved functional transduction efficiency together with a limited increase in the number of integrations per recipient cell, made us select Phoenix clone 58 for our clinical immunogene therapy study...
  66. ncbi An immunomagnetic epithelial tumor cell enrichment model for minimal residual disease detection of cytokeratin 8+ malignancies
    R A Brooimans
    Laboratory of Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Biol Regul Homeost Agents 19:84-91. 2005
    ..However, performance of the assay on cryopreserved and thawed cells needs to be improved...
  67. doi Circulating endothelial cells, circulating tumour cells, tissue factor, endothelin-1 and overall survival in prostate cancer patients treated with docetaxel
    M H Strijbos
    Department of Medical Oncology, Erasmus Medical Center, Rotterdam, The Netherlands
    Eur J Cancer 46:2027-35. 2010
    ..Additionally, we combined these markers with circulating tumour cells (CTCs) to construct a predictive nomogram for treatment outcome...
  68. ncbi Retronectin-assisted retroviral transduction of primary human T lymphocytes under good manufacturing practice conditions: tissue culture bag critically determines cell yield
    C H J Lamers
    Laboratory of Clinical and Tumor Immunology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cytotherapy 10:406-16. 2008
    ..Critical to our successful clinical-scale transductions of patient T cells was the use of Retronectin in combination with Lifecell X-foldtrade mark cell culture bags...
  69. doi Retroviral vectors for clinical immunogene therapy are stable for up to 9 years
    C H J Lamers
    Department of Medical Oncology, Laboratory of Clinical and Tumor Immunology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cancer Gene Ther 15:268-74. 2008
    ..These data provide evidence that in terms of 'life expectancy' the production and storage of clinical batches of RTVsup for gene therapy warrants the corresponding professional and financial risks...
  70. ncbi Enumeration of antigen-specific CD8+ T lymphocytes by single-platform, HLA tetramer-based flow cytometry: a European multicenter evaluation
    Ingmar A F M Heijnen
    Division of Immunology, Department of Laboratory Medicine, Kantonsspital Aarau, Aarau, Switzerland
    Cytometry B Clin Cytom 62:1-13. 2004
    ..Hence, the European Working Group on Clinical Cell Analysis set out to develop and evaluate a single-platform tetramer-based method that used cytomegalovirus (CMV) as the antigenic model...
  71. doi Dissection of the CMV specific T-cell response is required for optimized cardiac transplant monitoring
    Alexander Kirchner
    Institut fur Medizinische Immunologie, Charite, Berlin, Germany
    J Med Virol 80:1604-14. 2008
    ..These results should be used to inform the design of clinically applicable and diagnostically useful approaches to monitoring CMV specific responses in heart transplant recipients...
  72. ncbi Long wavelength fluorophores and cell-by-cell correction for autofluorescence significantly improves the accuracy of flow cytometric energy transfer measurements on a dual-laser benchtop flow cytometer
    Zsolt Sebestyen
    Department of Biophysics and Cell Biology, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary
    Cytometry 48:124-35. 2002
    ..However, the requirement for a dual-laser instrument and the need for a relatively high signal-to-noise system (i.e., high expression level of the molecules) pose limitations to a wide application of the method...
  73. ncbi Potential biomarkers in metastatic gastrointestinal stromal tumors
    Michiel H Strijbos
    Clin Cancer Res 13:6244. 2007
  74. ncbi Measuring Ag-specific immune responses: understanding immunopathogenesis and improving diagnostics in infectious disease, autoimmunity and cancer
    Florian Kern
    Institut fur Medizinische Immunologie, Charite Universitatsmedizin Berlin, Campus Mitte, 10098 Berlin, Germany
    Trends Immunol 26:477-84. 2005
    ....
  75. doi Enumeration of CD34+ hematopoietic stem and progenitor cells
    D Robert Sutherland
    The University Health Network, Toronto, Ontario, Canada
    Curr Protoc Cytom . 2003
    ..All the possible problems are discussed and examples provided, allowing the clinical hematology lab to be certain that analysis of CD34(+) cells is performed in an appropriate manner...
  76. ncbi Critical role of flow cytometry in evaluating peripheral blood hematopoietic stem cell grafts
    Michael Keeney
    The London Health Sciences Centre, London, Ontario, Canada
    Cytometry A 58:72-5. 2004