Alex van Belkum

Summary

Affiliation: Erasmus MC
Country: The Netherlands

Publications

  1. ncbi request reprint Guidelines for the validation and application of typing methods for use in bacterial epidemiology
    A van Belkum
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    Clin Microbiol Infect 13:1-46. 2007
  2. pmc Fluoroquinolone-resistant Escherichia coli, Indonesia
    Kuntaman Kuntaman
    Airlangga University, Surabaya, Indonesia
    Emerg Infect Dis 11:1363-9. 2005
  3. pmc Neopterin and procalcitonin are suitable biomarkers for exclusion of severe Plasmodium falciparum disease at the initial clinical assessment of travellers with imported malaria
    René te Witt
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Centre, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Malar J 9:255. 2010
  4. pmc Microbial DNA fingerprinting of human fingerprints: dynamic colonization of fingertip microflora challenges human host inferences for forensic purposes
    Sebastian Tims
    Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    Int J Legal Med 124:477-81. 2010
  5. pmc Comparative genomic analysis of Campylobacter jejuni associated with Guillain-Barré and Miller Fisher syndromes: neuropathogenic and enteritis-associated isolates can share high levels of genomic similarity
    Eduardo N Taboada
    Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, Ontario, K1A 0R6, Canada
    BMC Genomics 8:359. 2007
  6. ncbi request reprint Tracing isolates of bacterial species by multilocus variable number of tandem repeat analysis (MLVA)
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 49:22-7. 2007
  7. ncbi request reprint Staphylococcal colonization and infection: homeostasis versus disbalance of human (innate) immunity and bacterial virulence
    Alex van Belkum
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Rotterdam, The Netherlands
    Curr Opin Infect Dis 19:339-44. 2006
  8. doi request reprint Co-evolutionary aspects of human colonisation and infection by Staphylococcus aureus
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Erasmus MC, Rotterdam, The Netherlands
    Infect Genet Evol 9:32-47. 2009
  9. pmc Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    J Clin Microbiol 45:2698-700. 2007
  10. ncbi request reprint Molecular diagnostics in medical microbiology: yesterday, today and tomorrow
    Alex van Belkum
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Dr Molewaterplein 40, 3015 GD Rotterdam, Netherlands
    Curr Opin Pharmacol 3:497-501. 2003

Detail Information

Publications115 found, 100 shown here

  1. ncbi request reprint Guidelines for the validation and application of typing methods for use in bacterial epidemiology
    A van Belkum
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    Clin Microbiol Infect 13:1-46. 2007
    ..A strategic effort on the part of all workers in the field is thus mandatory to combat emerging infectious diseases, as is financial support from national and international granting bodies and health authorities...
  2. pmc Fluoroquinolone-resistant Escherichia coli, Indonesia
    Kuntaman Kuntaman
    Airlangga University, Surabaya, Indonesia
    Emerg Infect Dis 11:1363-9. 2005
    ..Hypervirulent strains from the B2 cluster were underrepresented (1%). Because FQ-resistant E. coli can cause disease, especially nosocomial infections in immunocompromised patients, spread of such strains must be stopped...
  3. pmc Neopterin and procalcitonin are suitable biomarkers for exclusion of severe Plasmodium falciparum disease at the initial clinical assessment of travellers with imported malaria
    René te Witt
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Centre, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Malar J 9:255. 2010
    ..falciparum malaria, 64 patients with uncomplicated P. falciparum malaria and 14 patients with severe P. falciparum malaria)...
  4. pmc Microbial DNA fingerprinting of human fingerprints: dynamic colonization of fingertip microflora challenges human host inferences for forensic purposes
    Sebastian Tims
    Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    Int J Legal Med 124:477-81. 2010
    ..Overall, our results suggest that human fingerprint microflora is too dynamic to allow for forensic marker developments for retrieving human information...
  5. pmc Comparative genomic analysis of Campylobacter jejuni associated with Guillain-Barré and Miller Fisher syndromes: neuropathogenic and enteritis-associated isolates can share high levels of genomic similarity
    Eduardo N Taboada
    Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, Ontario, K1A 0R6, Canada
    BMC Genomics 8:359. 2007
    ..jejuni factors associated with GBS/MFS to date and has allowed us to analyze the genetic background of neuropathogenic C. jejuni strains with an unprecedented level of resolution...
  6. ncbi request reprint Tracing isolates of bacterial species by multilocus variable number of tandem repeat analysis (MLVA)
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 49:22-7. 2007
    ..MLVA is a modern, timely and versatile bacterial typing methodology...
  7. ncbi request reprint Staphylococcal colonization and infection: homeostasis versus disbalance of human (innate) immunity and bacterial virulence
    Alex van Belkum
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Rotterdam, The Netherlands
    Curr Opin Infect Dis 19:339-44. 2006
    ..When nasal colonization is in the neutral state, infection clearly represents a state of host-pathogen disbalance. The features leading from apparent homeostatic colonization to pathogenesis are identified at an increasing rate...
  8. doi request reprint Co-evolutionary aspects of human colonisation and infection by Staphylococcus aureus
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Erasmus MC, Rotterdam, The Netherlands
    Infect Genet Evol 9:32-47. 2009
    ..When the nature of these states would be known in more detail, this knowledge could be used to design novel and empirical, knowledge-driven means of preventing colonisation from proceeding into S. aureus infection...
  9. pmc Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    J Clin Microbiol 45:2698-700. 2007
    ..Samples containing 10(2) to 10(3) MRSA cells were frequently reported to be negative. MRSE samples were scored as negative by all commercial tests but by only two out of three in-house tests...
  10. ncbi request reprint Molecular diagnostics in medical microbiology: yesterday, today and tomorrow
    Alex van Belkum
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Dr Molewaterplein 40, 3015 GD Rotterdam, Netherlands
    Curr Opin Pharmacol 3:497-501. 2003
    ..Quantitative amplification tests in combination with genomics, transcriptomics, proteomics and related methodologies will pave the way to further enhancement of innovative microbial detection and identification...
  11. pmc Unusually high prevalence of panton-valentine leukocidin genes among methicillin-sensitive Staphylococcus aureus strains carried in the Indonesian population
    Juliette A Severin
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, s Gravendijkwal 230, Rotterdam, The Netherlands
    J Clin Microbiol 46:1989-95. 2008
    ..The high prevalence of PVL-positive S. aureus strains in certain regions of Indonesia is of concern since these strains may cause severe infections in the community and in hospitals...
  12. doi request reprint Host-microbe interplay in persistent Staphylococcus aureus nasal carriage in HIV patients
    Damian C Melles
    Department of Medical Microbiology and Infectious Diseases, room L 313, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Microbes Infect 10:151-8. 2008
    ..2%) and no PVL positive strains were detected. The population structure of S. aureus strains isolated from HIV patients appeared to be strongly overlapping with that of S. aureus isolates from healthy individuals...
  13. doi request reprint Preventing surgical-site infections in nasal carriers of Staphylococcus aureus
    Lonneke G M Bode
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands
    N Engl J Med 362:9-17. 2010
    ..Nasal carriers of Staphylococcus aureus are at increased risk for health care-associated infections with this organism. Decolonization of nasal and extranasal sites on hospital admission may reduce this risk...
  14. ncbi request reprint Associations between Staphylococcus aureus Genotype, Infection, and In-Hospital Mortality: A Nested Case-Control Study
    Heiman F L Wertheim
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Infect Dis 192:1196-200. 2005
    ..Those infected with S. aureus strains belonging to a CC had higher mortality than those infected with strains not belonging to a CC (P<.05), which indicates the coevolution of S. aureus virulence and spread in humans...
  15. pmc The inverse correlation between Staphylococcus aureus and Streptococcus pneumoniae colonization in infants is not explained by differences in serum antibody levels in the Generation R Study
    Ankie Lebon
    The Generation R Study Group, Erasmus Medical Center Erasmus MC, Rotterdam, Netherlands
    Clin Vaccine Immunol 18:180-3. 2011
    ..In the first year of life, no association between maternal IgG levels and colonization was seen. In addition, no association between the IgG and IgA levels in the child versus colonization status was seen...
  16. pmc Comparative analysis of the humoral immune response to Moraxella catarrhalis and Streptococcus pneumoniae surface antigens in children suffering from recurrent acute otitis media and chronic otitis media with effusion
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Vaccine Immunol 19:914-8. 2012
    ..Other factors, such as Eustachian tube dysfunction, viral load, and genetic and environmental factors, may play a more important role in the pathogenesis of OM and in particular in the development of rAOM or COME...
  17. pmc Genotyping of Madurella mycetomatis by selective amplification of restriction fragments (amplified fragment length polymorphism) and subtype correlation with geographical origin and lesion size
    Wendy W J van de Sande
    Erasmus MC University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, The Netherlands
    J Clin Microbiol 43:4349-56. 2005
    ..001). This is the first report on a genotypic study where genetic markers which may be used to study pathogenicity in M. mycetomatis were obtained...
  18. doi request reprint Colonization of healthy children by Moraxella catarrhalis is characterized by genotype heterogeneity, virulence gene diversity and co-colonization with Haemophilus influenzae
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Microbiology 157:169-78. 2011
    ..catarrhalis isolates, may be a result of immune evasion by this pathogen. Finally, the factors facilitating M. catarrhalis and H. influenzae co-colonization need to be further investigated...
  19. doi request reprint Anti-staphylococcal humoral immune response in persistent nasal carriers and noncarriers of Staphylococcus aureus
    Nelianne J Verkaik
    Departments of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands
    J Infect Dis 199:625-32. 2009
    ..Persistent carriers have a higher risk of Staphylococcus aureus infections than noncarriers but a lower risk of bacteremia-related death. Here, the role played by anti-staphylococcal antibodies was studied...
  20. doi request reprint Molecular characterization of extended-spectrum beta-lactamases in clinical Escherichia coli and Klebsiella pneumoniae isolates from Surabaya, Indonesia
    Juliette A Severin
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, s Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands
    J Antimicrob Chemother 65:465-9. 2010
    ..No detailed reports regarding extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are currently available from Indonesia, the fourth most populous country in the world...
  21. pmc Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barré syndrome by high-throughput AFLP analysis
    Peggy C R Godschalk
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center Rotterdam, Dr, Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    BMC Microbiol 6:32. 2006
    ..This study describes a new C. jejuni-specific high-throughput AFLP (htAFLP) approach for detection and identification of DNA polymorphism, in general, and of putative GBS/MFS-markers, in particular...
  22. pmc Host- and tissue-specific pathogenic traits of Staphylococcus aureus
    Willem B van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Bacteriol 187:4584-91. 2005
    ..Mastitis-associated S. aureus isolated in diverse farm animal species form a distinct genetic cluster, characterized by an overrepresentation of the toxic shock syndrome toxin superantigen-encoding gene...
  23. pmc Clonal distribution and differential occurrence of the enterotoxin gene cluster, egc, in carriage- versus bacteremia-associated isolates of Staphylococcus aureus
    Alex van Belkum
    Erasmus MC, University Medical Center Rotterdam, room L 313, Department of Medical Microbiology and Infectious Diseases, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 44:1555-7. 2006
    ..7% of carriage-associated isolates versus 52.9% of invasive isolates; P = 0.03). Multilocus sequence typing revealed that successful staphylococcal clones usually harbor the egc locus...
  24. pmc Characterization of the humoral immune response during Staphylococcus aureus bacteremia and global gene expression by Staphylococcus aureus in human blood
    Paul Martijn den Reijer
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    PLoS ONE 8:e53391. 2013
    ..In addition, the expression of these antigens by S. aureus is not significantly altered by incubation in human blood over time. One immunogenic and ubiquitously expressed antigen is the putative iron-regulated ABC transporter SA0688...
  25. ncbi request reprint Faecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae among humans in Java, Indonesia, in 2001-2002
    Juliette A Severin
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Trop Med Int Health 17:455-61. 2012
    ....
  26. pmc Methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 in pigs and humans
    Alex van Belkum
    University Medical Center Rotterdam, Rotterdam, The Netherlands
    Emerg Infect Dis 14:479-83. 2008
    ..aureus circulates among humans at low frequency (0.2%) but was isolated in 3 human cases of bacteremia (2.1%; p = 0.026). Although its natural host is probably porcine, ST398 MRSA likely causes infections in humans...
  27. pmc Characterization of the specific interaction between sialoadhesin and sialylated Campylobacter jejuni lipooligosaccharides
    Astrid P Heikema
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, Netherlands
    Infect Immun 78:3237-46. 2010
    ....
  28. ncbi request reprint Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus
    Damian C Melles
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 69:371-5. 2007
    ..Epidemiological conditions should determine which is the optimal typing method to be used...
  29. pmc Natural antibodies against several pneumococcal virulence proteins in children during the pre-pneumococcal-vaccine era: the generation R study
    Ankie Lebon
    The Generation R Study Group, Erasmus MC, Rotterdam, The Netherlands
    Infect Immun 79:1680-7. 2011
    ..Certain antibodies against pneumococcal virulence proteins, some of which are induced by colonization, are associated with a decreased number of RTIs in children. This should be taken into account in future pneumococcal vaccine studies...
  30. ncbi request reprint The role of human innate immune factors in nasal colonization by Staphylococcus aureus
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Microbes Infect 9:1471-7. 2007
    ..aureus carriage predisposition. However, MBL haplotypes do so significantly. Baseline HNP1-3 production is more the consequence of S. aureus colonization than a reason for the (non) carrier status...
  31. doi request reprint Reclassification of Staphylococcus aureus nasal carriage types
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Infect Dis 199:1820-6. 2009
    ..This study was performed to provide additional insight into staphylococcal carriage types...
  32. ncbi request reprint Nasopharyngeal co-colonization with Staphylococcus aureus and Streptococcus pneumoniae in children is bacterial genotype independent
    Damian C Melles
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Microbiology 153:686-92. 2007
    ..pneumoniae subtypes. Further studies should demonstrate whether differences in bacterial interference are due to more subtle genetic changes...
  33. pmc PCR-restriction fragment length polymorphism analysis of Campylobacter jejuni genes involved in lipooligosaccharide biosynthesis identifies putative molecular markers for Guillain-Barré syndrome
    Peggy C R Godschalk
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Clin Microbiol 45:2316-20. 2007
    ..PCR-restriction fragment length polymorphism analysis of C. jejuni genes involved in the biosynthesis of LOS demonstrated that specific genes were associated with the expression of ganglioside mimics and the development of neuropathy...
  34. pmc Overlapping population structures of nasal isolates of Staphylococcus aureus from healthy Dutch and American individuals
    Damian C Melles
    Erasmus MC, University Medical Center Rotterdam, room L 313, Department of Medical Microbiology and Infectious Diseases, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    J Clin Microbiol 46:235-41. 2008
    ..In conclusion, the overall population structures of American and Dutch nasal carriage isolates of S. aureus are surprisingly similar, despite subtle geographic differences in the prevalence of certain S. aureus genotypes...
  35. pmc Key role for clumping factor B in Staphylococcus aureus nasal colonization of humans
    Heiman F L Wertheim
    Erasmus MC, University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    PLoS Med 5:e17. 2008
    ..aureus colonizes the nose are still unknown. The staphylococcal cell-wall protein clumping factor B (ClfB) promotes adhesion to squamous epithelial cells in vitro and might be a physiologically relevant colonization factor...
  36. doi request reprint Temporal development of the humoral immune response to surface antigens of Moraxella catarrhalis in young infants
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Vaccine 29:5603-10. 2011
    ..catarrhalis has not matured at 2 years of age. We provide evidence that a humoral antibody response to OMPs UspA1, UspA2 and Hag/MID may play a role in the immune response to community acquired M. catarrhalis colonization events...
  37. doi request reprint Analysis of VanA vancomycin-resistant Enterococcus faecium isolates from Saudi Arabian hospitals reveals the presence of clonal cluster 17 and two new Tn1546 lineage types
    Mushtaq A Khan
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, s Gravendijkwal 230, 3015CE Rotterdam, The Netherlands
    J Antimicrob Chemother 62:279-83. 2008
    ..The aim of this study was to characterize 34 vancomycin-resistant VanA Enterococcus faecium isolates obtained from two hospitals in Saudi Arabia and to assess Tn1546 variation within these isolates...
  38. ncbi request reprint Staphylococcus aureus nasal carriage is not associated with known polymorphism in the Vitamin D receptor gene
    Mark Claassen
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 43:173-6. 2005
    ..Our study into genetic determinants of S. aureus carriage patterns is the largest in the field, but still we found no association between VDR gene variation and S. aureus nasal carriage...
  39. pmc Correlation of bacterial colonization status between mother and child: the Generation R Study
    Ankie Lebon
    Department of Paediatrics, Erasmus Medical Center Sophia Children s Hospital, Rotterdam, Netherlands
    J Clin Microbiol 48:960-2. 2010
    ..Such a correlation was observed for Staphylococcus aureus and Haemophilus influenzae. Direct transmission, genetic susceptibility and/or unidentified environmental factors may play a role here...
  40. ncbi request reprint Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit
    Roos S R Choudhurry
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 65:354-6. 2006
    ..However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers...
  41. doi request reprint A multiplex assay for the quantification of antibody responses in Staphylococcus aureus infections in mice
    Sanne van den Berg
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, 3015 CE Rotterdam, The Netherlands
    J Immunol Methods 365:142-8. 2011
    ..aureus antibody assay can be successfully applied to determine the immunogenicity of different S. aureus proteins in relation to the site of infection and the S. aureus strain causing the infection...
  42. doi request reprint Serotyping of Dutch Staphylococcus aureus strains from carriage and infection
    Damian C Melles
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 52:287-92. 2008
    ..Thus, addition of the 336PS conjugate to a type 5- and type 8-CP protein conjugate vaccine would significantly extend the vaccine coverage...
  43. ncbi request reprint A novel plasmid (pEMCJH03) isolated from moraxella catarrhalis possibly useful as a cloning and expression vector within this species
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC Erasmus University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Plasmid 53:263-8. 2005
    ..catarrhalis using pEMCJH04 could help in the development of a vaccine expression vector, as well as providing a useful tool for studying promoter activity and in complementation studies of gene knockout mutants...
  44. pmc Rapid typing of extended-spectrum β-lactamase- and carbapenemase-producing Escherichia coli and Klebsiella pneumoniae isolates by use of SpectraCell RA
    Diana Willemse-Erix
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center, Rotterdam, Netherlands
    J Clin Microbiol 50:1370-5. 2012
    ..coli and K. pneumoniae isolates. Combining high reproducibility, speed, and ease-of-use, this technique may play an important role in monitoring the epidemiology of these important nosocomial species...
  45. pmc Rhesus macaques (Macaca mulatta) are natural hosts of specific Staphylococcus aureus lineages
    Sanne van den Berg
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    PLoS ONE 6:e26170. 2011
    ..In conclusion, rhesus macaques are natural hosts of S. aureus, carrying host-specific lineages. Our data indicate that rhesus macaques are useful as an autologous model for studying S. aureus nasal colonization and infection prevention...
  46. doi request reprint 5 years of experience implementing a methicillin-resistant Staphylococcus aureus search and destroy policy at the largest university medical center in the Netherlands
    Margreet C Vos
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, The Netherlands
    Infect Control Hosp Epidemiol 30:977-84. 2009
    ..To evaluate the effectiveness of a rigorous search and destroy policy for controlling methicillin-resistant Staphylococcus aureus (MRSA) infection or colonization...
  47. doi request reprint Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001-2002
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Microbiology 154:1178-84. 2008
    ..Our results should also be useful in the choice of effective vaccine candidates against M. catarrhalis...
  48. pmc The sialylated lipooligosaccharide outer core in Campylobacter jejuni is an important determinant for epithelial cell invasion
    Rogier Louwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Infect Immun 76:4431-8. 2008
    ..We conclude that sialylation of the LOS outer core contributes significantly to epithelial invasion by C. jejuni and may thus play a role in subsequent postinfectious pathologies...
  49. ncbi request reprint Total genome polymorphism and low frequency of intra-genomic variation in the uspA1 and uspA2 genes of Moraxella catarrhalis in otitis prone and non-prone children up to 2 years of age. Consequences for vaccine design?
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Vaccine 21:1118-24. 2003
    ..Variation in the uspA2 5' non-coding "AGAT" repeat region was also observed. These results may have implications for future M. catarrhalis vaccines comprising UspA1 or UspA2 components...
  50. ncbi request reprint A new high-throughput AFLP approach for identification of new genetic polymorphism in the genome of the clonal microorganism Mycobacterium tuberculosis
    Nicole van den Braak
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    J Microbiol Methods 56:49-62. 2004
    ..tuberculosis. The tests allowed for identification of the major Mycobacterium species and M. tuberculosis variants and clones...
  51. ncbi request reprint Epidemiology of Staphylococcus aureus harboring the mecA or Panton-Valentine leukocidin genes in hospitals in Java and Bali, Indonesia
    Dewi Santosaningsih
    Department of Microbiology, Faculty of Medicine, Brawijaya University Dr Saiful Anwar Hospital, Malang, Indonesia Department of Microbiology, Faculty of Medicine, Udayana University Sanglah Hospital, Denpasar, Bali, Indonesia Department of Microbiology, Faculty of Medicine, Airlangga University Dr Soetomo Hospital, Surabaya, Indonesia Department of Microbiology, Faculty of Medicine, Diponegoro University Dr Kariadi Hospital, Semarang, Indonesia Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands
    Am J Trop Med Hyg 90:728-34. 2014
    ..In conclusion, MRSA and PVL-positive MSSA are present among patients in surgical wards in Indonesian academic hospitals...
  52. pmc Candida krusei transmission among hematology patients resolved by adapted antifungal prophylaxis and infection control measures
    Margreet C Vos
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 44:1111-4. 2006
    ..We intervened by changing antifungal prophylaxis and implementing strict infection control measures. The incidence dropped immediately...
  53. ncbi request reprint Rifampicin-induced transcriptome response in rifampicin-resistant Mycobacterium tuberculosis
    Gerjo J de Knegt
    Erasmus MC, University Medical Centre Rotterdam, Department of Medical Microbiology and Infectious Diseases, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Tuberculosis (Edinb) 93:96-101. 2013
    ..tuberculosis. Further investigation of Rv0559c and Rv0560c is needed to reveal function and mechanism of both genes that were triggered upon rifampicin exposure...
  54. doi request reprint Procalcitonin and neopterin levels do not accurately distinguish bacterial from viral infections in ill-returned travellers with fever
    René te Witt
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Trans R Soc Trop Med Hyg 106:264-6. 2012
    ....
  55. doi request reprint bro {beta}-lactamase and antibiotic resistances in a global cross-sectional study of Moraxella catarrhalis from children and adults
    Mushtaq A Khan
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Antimicrob Chemother 65:91-7. 2010
    ..To compare and contrast the geographic and demographic distribution of bro beta-lactamase and antibiotic MIC(50/90) for 1440 global Moraxella catarrhalis isolates obtained from children and adults between 2001 and 2002...
  56. pmc Natural population dynamics and expansion of pathogenic clones of Staphylococcus aureus
    Damian C Melles
    University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, room L 313, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Clin Invest 114:1732-40. 2004
    ..We provide evidence that essentially any S. aureus genotype carried by humans can transform into a life-threatening human pathogen but that certain clones are more virulent than others...
  57. ncbi request reprint The role of nasal carriage in Staphylococcus aureus infections
    Heiman F L Wertheim
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, Netherlands
    Lancet Infect Dis 5:751-62. 2005
    ..We present an overview of the current knowledge of the determinants (both human and bacterial) and risks of S aureus nasal carriage. Studies on the population dynamics of S aureus are also summarised...
  58. doi request reprint Immunogenicity of toxins during Staphylococcus aureus infection
    Nelianne J Verkaik
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands
    Clin Infect Dis 50:61-8. 2010
    ..Toxins are important Staphylococcus aureus virulence factors, but little is known about their immunogenicity during infection. Here, additional insight is generated...
  59. pmc Dynamics and determinants of Staphylococcus aureus carriage in infancy: the Generation R Study
    Ankie Lebon
    The Generation R Study Group, Erasmus Medical Center, Rotterdam, The Netherlands
    J Clin Microbiol 46:3517-21. 2008
    ..1% at the age of 1.5 months to 12.9% at 14 months. Persistent carriage, defined as continuous carriage of the same S. aureus strain at the three sampling moments, was rarely detected in early infancy...
  60. pmc Severity of nonbullous Staphylococcus aureus impetigo in children is associated with strains harboring genetic markers for exfoliative toxin B, Panton-Valentine leukocidin, and the multidrug resistance plasmid pSK41
    Sander Koning
    Department of General Practice, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 41:3017-21. 2003
    ..The identification of these microbial genetic markers, which are predictive of the severity and the course of the disease, will facilitate guided individualized antimicrobial therapy in the future...
  61. ncbi request reprint Staphylococcus aureus nasal carriage is associated with glucocorticoid receptor gene polymorphisms
    Erica L T Van Den Akker
    Department of Internal Medicine, Erasmus Medical Center, University Medical Center, Rotterdam, The Netherlands
    J Infect Dis 194:814-8. 2006
    ..Thus, genotype-dependent variation in the sensitivity to glucocorticoids is associated with tolerance toward staphylococcal nasal colonization...
  62. ncbi request reprint TLR4-mediated sensing of Campylobacter jejuni by dendritic cells is determined by sialylation
    Mark L Kuijf
    Department of Neurology, Erasmus Medical Center, University Medical Center, Rotterdam, The Netherlands
    J Immunol 185:748-55. 2010
    ..jejuni LOS enhances human DC activation and subsequent B cell proliferation, which may contribute to the development of cross-reactive anti-ganglioside Abs found in GBS patients following C. jejuni infection...
  63. ncbi request reprint Towards Raman-based epidemiological typing of Pseudomonas aeruginosa
    Diana F M Willemse-Erix
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands
    J Biophotonics 3:506-11. 2010
    ..It will be important to analyze the range of the spectral variations that can occur and ensure the correction of all of these factors to obtain the highest reproducibility required for strain level typing...
  64. pmc Survival of Staphylococcus aureus ST398 in the human nose after artificial inoculation
    Bibi C G C Slingerland
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    PLoS ONE 7:e48896. 2012
    ..In conclusion, S. aureus ST398 strain 5062 is capable of adequately competing for a niche with a human strain and survives in the human nose for at least 21 days...
  65. pmc Campylobacter jejuni translocation across intestinal epithelial cells is facilitated by ganglioside-like lipooligosaccharide structures
    Rogier Louwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Infect Immun 80:3307-18. 2012
    ..jejuni ganglioside-like LOS-expressing isolates are linked with severe gastroenteritis and bloody stools in C. jejuni-infected patients...
  66. pmc Effect of mupirocin treatment on nasal, pharyngeal, and perineal carriage of Staphylococcus aureus in healthy adults
    Heiman F L Wertheim
    Erasmus MC, University Hospital Rotterdam, Department of Clinical Microbiology and Infectious Diseases, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Antimicrob Agents Chemother 49:1465-7. 2005
    ..The finding warrants the use of mupirocin only in proven carriers for decolonization purposes. Mupirocin is effective overall in decolonizing nasal carriers but less effective in decolonizing extranasal sites...
  67. ncbi request reprint Translationally controlled tumor protein from Madurella mycetomatis, a marker for tumorous mycetoma progression
    Wendy W J van de Sande
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Centre Rotterdam, Rotterdam, The Netherlands
    J Immunol 177:1997-2005. 2006
    ..After 6-15 years of disease duration the Ab levels were the highest. TCTP is the first well-characterized immunogenic Ag, simultaneously the first monomolecular vaccine candidate, identified for the fungus M. mycetomatis...
  68. ncbi request reprint Association between nasal carriage of Staphylococcus aureus and the human complement cascade activator serine protease C1 inhibitor (C1INH) valine vs. methionine polymorphism at amino acid position 480
    Marieke Emonts
    Department of Pediatrics, Erasmus MC Sophia, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 50:330-2. 2007
    ..52, P=0.02). These findings suggest that susceptibility to S. aureus nasal carriage is associated with the C1INH V480M polymorphism...
  69. ncbi request reprint Polymorphisms in genes involved in innate immunity predispose toward mycetoma susceptibility
    Wendy W J van de Sande
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands
    J Immunol 179:3065-74. 2007
    ..We present the first evidence of human genetic predisposition toward susceptibility to mycetoma, a neglected infection of the poor...
  70. ncbi request reprint Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers
    Heiman F L Wertheim
    Erasmus MC, University Medical Centre, Rotterdam, Department of Medical Microbiology and Infectious Diseases, Dr Molewaterplein 40, 3015 GD Rotterdam, Netherlands
    Lancet 364:703-5. 2004
    ..006). S aureus nasal carriers and non-carriers differ significantly in risk and outcome of nosocomial S aureus bacteraemia. Genotyping revealed that 80% of strains causing bacteraemia in carriers were endogenous...
  71. pmc Multilocus sequence typing of Staphylococcus aureus with DNA array technology
    Willem B van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 41:3323-6. 2003
    ..Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant...
  72. pmc Identification and characterization of a novel outer membrane protein (OMP J) of Moraxella catarrhalis that exists in two major forms
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Bacteriol 187:7977-84. 2005
    ....
  73. doi request reprint Polymorphisms in catechol-O-methyltransferase and cytochrome p450 subfamily 19 genes predispose towards Madurella mycetomatis-induced mycetoma susceptibility
    Wendy W J van de Sande
    Erasmus MC, University Medical Centre Rotterdam, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    Med Mycol 48:959-68. 2010
    ..Indirect effects on the patients' hormone regulated immune states are the more likely explanations for mycetoma susceptibility...
  74. pmc Structural characterization of Campylobacter jejuni lipooligosaccharide outer cores associated with Guillain-Barre and Miller Fisher syndromes
    Peggy C R Godschalk
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Infect Immun 75:1245-54. 2007
    ..jejuni-related GBS. However, our findings suggest that in some cases, other mechanisms may play a role. Further examination of the disease etiology in these patients is mandatory...
  75. pmc Madurella mycetomatis is not susceptible to the echinocandin class of antifungal agents
    Wendy W J van de Sande
    Erasmus MC, University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases CE Rotterdam, The Netherlands
    Antimicrob Agents Chemother 54:2738-40. 2010
    ..As a comparator fungus, Aspergillus fumigatus was used. Minimal effective concentrations (MECs) and MICs were assessed and compared to those of ketoconazole. M. mycetomatis isolates were not susceptible to the echinocandins...
  76. ncbi request reprint Persistent (not intermittent) nasal carriage of Staphylococcus aureus is the determinant of CPD-related infections
    Jan L Nouwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, The Netherlands
    Kidney Int 67:1084-92. 2005
    ..We investigated the impact of staphylococcal carriage among patients on continuous peritoneal dialysis (CPD) in a university hospital...
  77. pmc Staphylococcus aureus carriage patterns and the risk of infections associated with continuous peritoneal dialysis
    Jan Nouwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands
    J Clin Microbiol 44:2233-6. 2006
    ..No methicillin or vancomycin resistance was detected...
  78. ncbi request reprint Melanin biosynthesis in Madurella mycetomatis and its effect on susceptibility to itraconazole and ketoconazole
    Wendy W J van de Sande
    Erasmus MC, University Medical Centre Rotterdam, Department of Medical Microbiology and Infectious Diseases, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Microbes Infect 9:1114-23. 2007
    ..Since itraconazole and ketoconazole are the main antifungal agents used to treat mycetoma, the clinical relevance of the in vitro rise in MIC should be studied further...
  79. pmc Optical fingerprinting in bacterial epidemiology: Raman spectroscopy as a real-time typing method
    Diana F M Willemse-Erix
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands
    J Clin Microbiol 47:652-9. 2009
    ..This will set the stage for continuous, automated, real-time epidemiological monitoring of bacterial infections in a hospital, which can then be followed by timely corrective action by infection prevention teams...
  80. ncbi request reprint Strict infection control measures do not prevent clonal spread of coagulase negative staphylococci colonizing central venous catheters in neutropenic hemato-oncologic patients
    Cindy van Pelt
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 38:153-8. 2003
    ..In conclusion, despite a constant rate of CRI and implementation of optimal patient care, clonal spread of MRSE strains was not prevented by strict hygiene measures...
  81. doi request reprint Peptide-based fluorescence resonance energy transfer protease substrates for the detection and diagnosis of Bacillus species
    Wendy E Kaman
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Anal Chem 83:2511-7. 2011
    ..anthracis spores and diagnosis of anthrax infection is at hand. We are the first who present fluorogenic substrates for detection of bacterial proteolytic enzymes that can be directly applied in situ by the use of D-oriented amino acids...
  82. doi request reprint Time-kill kinetics of anti-tuberculosis drugs, and emergence of resistance, in relation to metabolic activity of Mycobacterium tuberculosis
    Jurriaan E M de Steenwinkel
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands
    J Antimicrob Chemother 65:2582-9. 2010
    ....
  83. doi request reprint Genotypes, superantigen gene profiles, and presence of exfoliative toxin genes in clinical methicillin-susceptible Staphylococcus aureus isolates
    Marijke J C A van Trijp
    Maasstad Hospital, HT Rotterdam, The Netherlands
    Diagn Microbiol Infect Dis 66:222-4. 2010
    ..Superantigen gene profiles and presence of exfoliative toxin genes A and D were associated with clonal complex rather than with invasive disease...
  84. ncbi request reprint Role of Staphylococcus aureus nasal colonization in atopic dermatitis in infants: the Generation R Study
    Ankie Lebon
    Department of Pediatrics, Erasmus Medical Center Sophia Children s Hospital, University Medical Center, PO Box 2060, 3000 CB Rotterdam, The Netherlands
    Arch Pediatr Adolesc Med 163:745-9. 2009
    ..To study the association between Staphylococcus aureus nasal colonization and atopic dermatitis (AD) in infancy...
  85. ncbi request reprint Intercenter reproducibility of binary typing for Staphylococcus aureus
    Willem B van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 51:19-28. 2002
    ..The current BT protocol thus represents a simple method generating robust, reproducible genotype data for S. aureus strains...
  86. pmc Isolation of nontuberculous mycobacteria in Zambia: eight case reports
    Patricia C A M Buijtels
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Centre Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 43:6020-6. 2005
    ..This is the first report of M. lentiflavum and M. goodii infections in Africa...
  87. pmc Testing of the in vitro susceptibilities of Madurella mycetomatis to six antifungal agents by using the Sensititre system in comparison with a viability-based 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XT
    Wendy W J van de Sande
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Antimicrob Agents Chemother 49:1364-8. 2005
    ..Based on the relatively broad range of MICs obtained for the antifungal agents, routine testing of M. mycetomatis isolates for susceptibility to antifungal agents seems to be relevant to adequate therapeutic management...
  88. pmc The crucial role of Campylobacter jejuni genes in anti-ganglioside antibody induction in Guillain-Barre syndrome
    Peggy C R Godschalk
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center, Rotterdam, The Netherlands
    J Clin Invest 114:1659-65. 2004
    ..We demonstrate, for the first time, to our knowledge, that specific bacterial genes are crucial for the induction of anti-ganglioside antibodies...
  89. ncbi request reprint Current trends in the epidemiological typing of clinically relevant microbes in Europe
    Kees Maquelin
    Center for Optical Diagnostics and Therapy, Department of General Surgery, Erasmus MC, University Medical Centre, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 69:222-6. 2007
    ..Genotyping by PFGE and MLST are currently clearly preferred over phenotyping. The overall wish is to increase the activities by over 20% and additional resources would be used to invest in real-time typing...
  90. pmc Human factor in Staphylococcus aureus nasal carriage
    Jan Nouwen
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Infect Immun 72:6685-8. 2004
    ..Using a human nasal inoculation model, we here demonstrate that the human factor is an important determinant of S. aureus nasal carriage...
  91. ncbi request reprint Moraxella catarrhalis is only a weak activator of the mannose-binding lectin (MBL) pathway of complement activation
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    FEMS Microbiol Lett 249:207-9. 2005
    ..Results showed that this organism is only a poor activator of the lectin pathway of complement activation, with subsequent consequences for the etiology of otitis media by this organism...
  92. doi request reprint Comparison of carboxylated and Penta-His microspheres for semi-quantitative measurement of antibody responses to His-tagged proteins
    Nelianne Verkaik
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Immunol Methods 335:121-5. 2008
    ..Therefore, we conclude that for developing a multiplex assay for semi-quantitative measurement of antibody responses against His-tagged proteins the best microspheres to use are the carboxylated ones...
  93. pmc Risk factors associated with Campylobacter jejuni infections in Curaçao, Netherlands Antilles
    Hubert P Endtz
    Department of Neurology, Erasmus MC, University Medical Center Rotterdam, The Netherlands
    J Clin Microbiol 41:5588-92. 2003
    ..Two out of three GBS-related C. jejuni isolates belonged to an epidemic clone. The observations presented point toward water as a possible source of Campylobacter infections...
  94. pmc Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 43:3346-55. 2005
    ..All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time...
  95. ncbi request reprint Phylogenetic background and virulence profiles of fluoroquinolone-resistant clinical Escherichia coli isolates from the Netherlands
    James R Johnson
    Medical Service, Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    J Infect Dis 186:1852-6. 2002
    ..These FQREC resemble animal-source E. coli and presumably pose little threat to noncompromised hosts. Similar analysis of other FQREC is needed...
  96. pmc Patterns of resistance associated with integrons, the extended-spectrum beta-lactamase SHV-5 gene, and a multidrug efflux pump of Klebsiella pneumoniae causing a nosocomial outbreak
    Paul Gruteke
    Municipal Public Health Laboratory, Amsterdam Zuiderzee Hospital, Lelystad, The Netherlands
    J Clin Microbiol 41:1161-6. 2003
    ..In this particular outbreak, screening for strains for which ciprofloxacin MICs were > or =0.25 micro g/ml would in retrospect have been the most sensitive method for detection of the K. pneumoniae outbreak strain...
  97. pmc Loss of the mecA gene during storage of methicillin-resistant Staphylococcus aureus strains
    Arjanne van Griethuysen
    Department of Medical Microbiology and Medical Immunology, Internal Post Number 1630, Hospital Rijnstate Arnhem, P O Box 9025, 6800 EG Arnhem, The Netherlands
    J Clin Microbiol 43:1361-5. 2005
    ..Further analysis of 35 of these isolates confirmed loss of the mecA gene in 32 isolates. This finding has important implications for the management of strain collections...
  98. pmc Recent emergence of an epidemic clindamycin-resistant clone of Clostridium difficile among Polish patients with C. difficile-associated diarrhea
    Hanna Pituch
    Department of Medical Microbiology, Centre of Biostructure Research, The Medical University of Warsaw, Warsaw, Poland
    J Clin Microbiol 41:4184-7. 2003
    ..Apparently, enhanced local spread of CDAD-causing C. difficile may be restricted to a limited number of bacterial genotypes only...
  99. ncbi request reprint Molecular epidemiology of Staphylococcus aureus colonization in a burn center
    Mirjam Kooistra-Smid
    Regional Public Health Laboratory, van Ketwich Verschuurlaan 92, 9721 SW, Groningen, The Netherlands
    Burns 30:27-33. 2004
    ..aureus at admission developed burn-wound colonization with that genotype they carried in the nose or throat. Both carriage in patients and health care workers and auto-infection play a crucial role in (cross-) colonization events...
  100. pmc Molecular evidence for dissemination of unique Campylobacter jejuni clones in Curaçao, Netherlands Antilles
    Birgitta Duim
    Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands
    J Clin Microbiol 41:5593-7. 2003
    ..Furthermore, given the observation that C. jejuni-associated diseases appear to be more severe from November to February, it can be speculated that this may be due to the presence of virulent clones with a limited span of circulation...