A van Belkum

Summary

Affiliation: Erasmus University Medical Center
Country: The Netherlands

Publications

  1. pmc Evaluation of molecular typing methods in characterizing a European collection of epidemic methicillin-resistant Staphylococcus aureus strains: the HARMONY collection
    Barry D Cookson
    Laboratory of Hospital Infection, Centre for Infections, Health Protection Agency, London, United Kingdom
    J Clin Microbiol 45:1830-7. 2007
  2. ncbi Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus
    Damian C Melles
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 69:371-5. 2007
  3. doi First community-acquired meticillin-resistant Staphylococcus aureus in Malaysia
    Mariana Nor Shamsudin
    J Med Microbiol 57:1180-1. 2008
  4. pmc Rapid method for sensitive screening of oligosaccharide epitopes in the lipooligosaccharide from Campylobacter jejuni strains isolated from Guillain-Barré syndrome and Miller Fisher syndrome patients
    Monika Dzieciatkowska
    NRC Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario, Canada K1A 0R6
    J Clin Microbiol 46:3429-36. 2008
  5. ncbi Low-stringency single specific primer PCR, DNA sequencing and single-strand conformation polymorphism of PCR products for identification of genetic variants of human papillomavirus type 16
    A van Belkum
    University Hospital Rotterdam, Department of Bacteriology, Netherlands
    J Virol Methods 55:435-43. 1995
  6. ncbi The role of short sequence repeats in epidemiologic typing
    A van Belkum
    Erasmus University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, Room L333, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    Curr Opin Microbiol 2:306-11. 1999
  7. ncbi A clinical study on the association of Trichomonas vaginalis and Mycoplasma hominis infections in women attending a sexually transmitted disease (STD) outpatient clinic
    A van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 32:27-32. 2001
  8. ncbi Comparison of conventional and molecular methods for the detection of bacterial pathogens in sputum samples from cystic fibrosis patients
    A van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 27:51-7. 2000
  9. pmc Rapid emergence of ciprofloxacin-resistant enterobacteriaceae containing multiple gentamicin resistance-associated integrons in a Dutch hospital
    A van Belkum
    Eramus University Medical Center Rotterdam, The Netherlands
    Emerg Infect Dis 7:862-71. 2001
  10. ncbi Attachment of Staphylococcus aureus to eukaryotic cells and experimental pitfalls in staphylococcal adherence assays: a critical appraisal
    Alex van Belkum
    Deptartment Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    J Microbiol Methods 48:19-42. 2002

Detail Information

Publications122 found, 100 shown here

  1. pmc Evaluation of molecular typing methods in characterizing a European collection of epidemic methicillin-resistant Staphylococcus aureus strains: the HARMONY collection
    Barry D Cookson
    Laboratory of Hospital Infection, Centre for Infections, Health Protection Agency, London, United Kingdom
    J Clin Microbiol 45:1830-7. 2007
    ....
  2. ncbi Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus
    Damian C Melles
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 69:371-5. 2007
    ..Epidemiological conditions should determine which is the optimal typing method to be used...
  3. doi First community-acquired meticillin-resistant Staphylococcus aureus in Malaysia
    Mariana Nor Shamsudin
    J Med Microbiol 57:1180-1. 2008
  4. pmc Rapid method for sensitive screening of oligosaccharide epitopes in the lipooligosaccharide from Campylobacter jejuni strains isolated from Guillain-Barré syndrome and Miller Fisher syndrome patients
    Monika Dzieciatkowska
    NRC Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario, Canada K1A 0R6
    J Clin Microbiol 46:3429-36. 2008
    ..jejuni strains. The structure was elucidated using material from a single colony. The total time for sample preparation and MS analysis is less than 60 min...
  5. ncbi Low-stringency single specific primer PCR, DNA sequencing and single-strand conformation polymorphism of PCR products for identification of genetic variants of human papillomavirus type 16
    A van Belkum
    University Hospital Rotterdam, Department of Bacteriology, Netherlands
    J Virol Methods 55:435-43. 1995
    ..Results are discussed in the context of experimental variability of the procedures or genetic heterogeneity of HPV16 pools derived from cervical swabs...
  6. ncbi The role of short sequence repeats in epidemiologic typing
    A van Belkum
    Erasmus University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, Room L333, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    Curr Opin Microbiol 2:306-11. 1999
    ..Although microbial SSR genotypes have been used with increasing frequency for studying the epidemiology and evolution of microbial strains and isolates, such approaches should be used with caution...
  7. ncbi A clinical study on the association of Trichomonas vaginalis and Mycoplasma hominis infections in women attending a sexually transmitted disease (STD) outpatient clinic
    A van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 32:27-32. 2001
    ..hominis and sexual risk behavior (P=0.0004). M. hominis and sexual risk behavior were more closely associated than M. hominis and T. vaginalis. No indications were found for an enhanced pathogenicity by either of the symbionts...
  8. ncbi Comparison of conventional and molecular methods for the detection of bacterial pathogens in sputum samples from cystic fibrosis patients
    A van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 27:51-7. 2000
    ....
  9. pmc Rapid emergence of ciprofloxacin-resistant enterobacteriaceae containing multiple gentamicin resistance-associated integrons in a Dutch hospital
    A van Belkum
    Eramus University Medical Center Rotterdam, The Netherlands
    Emerg Infect Dis 7:862-71. 2001
    ....
  10. ncbi Attachment of Staphylococcus aureus to eukaryotic cells and experimental pitfalls in staphylococcal adherence assays: a critical appraisal
    Alex van Belkum
    Deptartment Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    J Microbiol Methods 48:19-42. 2002
    ..Specific emphasis is placed upon the pros and cons of the various artificial, mostly in vitro models employed to study the interaction between bacterial and human or animal cells...
  11. pmc Role of genomic typing in taxonomy, evolutionary genetics, and microbial epidemiology
    A van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
    Clin Microbiol Rev 14:547-60. 2001
    ..Recommendations for unification of the different fields through standardization of laboratory techniques are made...
  12. ncbi Second European Meeting on Molecular Diagnostics. 11-12 October, 2001, Scheveningen, The Netherlands
    Alex van Belkum
    Erasmus MC, University Medical Center Rotterdam, Department of Medical, Microbiology and Infectious Diseases, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Expert Rev Mol Diagn 2:9-12. 2002
  13. ncbi Short sequence repeats in microbial pathogenesis and evolution
    A van Belkum
    Erasmus University Medical Centre Rotterdam, Department of Medical Microbiology and Infectious Diseases, The Netherlands
    Cell Mol Life Sci 56:729-34. 1999
    ..Together, DNA repeat analysis in microbial species provides information on both functional and evolutionary aspects of genetic diversity among microbial isolates...
  14. ncbi High-throughput epidemiologic typing in clinical microbiology
    A van Belkum
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    Clin Microbiol Infect 9:86-100. 2003
    ..The current state of technology is summarized, and future perspectives are sketched...
  15. ncbi Occurrence and structure-function relationship of pentameric short sequence repeats in microbial genomes
    A van Belkum
    Erasmus University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, The Netherlands
    Res Microbiol 150:617-26. 1999
    ....
  16. ncbi Molecular epidemiology of methicillin-resistant Staphylococcus aureus strains: state of affairs and tomorrow' s possibilities
    A van Belkum
    Erasmus University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, The Netherlands
    Microb Drug Resist 6:173-88. 2000
    ..Means for integrated assessment of bacterial biology, epidemiology, and population structure will be discussed...
  17. pmc Variable number of tandem repeats in clinical strains of Haemophilus influenzae
    A van Belkum
    Department of Bacteriology, University Hospital Rotterdam, The Netherlands
    Infect Immun 65:5017-27. 1997
    ..One of the 5-nucleotide VNTRs proved to be hypervariable. This variability may reflect the molecular basis of a mechanism used by H. influenzae bacteria to successfully colonize and infect different human individuals...
  18. ncbi Polymerase chain reaction-mediated typing of microorganisms: tracking dissemination of genes and genomes
    A van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center Rotterdam, The Netherlands
    Electrophoresis 19:602-7. 1998
    ..Technical limitations and possibilities for improvement will be discussed...
  19. pmc Dissemination of a single clone of methicillin-resistant Staphylococcus aureus among Turkish hospitals
    A van Belkum
    Department of Medical Microbiology and Infectious Diseases, University Hospital Rotterdam, The Netherlands
    J Clin Microbiol 35:978-81. 1997
    ..It is concluded that a major clone of MRSA has spread through a large part of Turkey, causing longitudinally persistent colonization in all of the institutions surveyed...
  20. pmc Short-sequence DNA repeats in prokaryotic genomes
    A van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
    Microbiol Mol Biol Rev 62:275-93. 1998
    ....
  21. pmc Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study
    A van Belkum
    Erasmus Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, The Netherlands
    J Clin Microbiol 36:1653-9. 1998
    ..In conclusion, it appears that the standardization of PFGE depends on controlling a variety of experimental intricacies, as is the case with other bacterial typing procedures...
  22. pmc Novel BOX repeat PCR assay for high-resolution typing of Streptococcus pneumoniae strains
    A van Belkum
    Department of Clinical Microbiology, University Hospital Rotterdam, Erasmus University, The Netherlands
    J Clin Microbiol 34:1176-9. 1996
    ..Considering the combined data from the five typing techniques applied previously as the "gold standard," the single BOX PCR test demonstrated excellent resolving powers while maintaining epidemiological linkage...
  23. pmc Outbreak of amoxicillin-resistant Haemophilus influenzae type b: variable number of tandem repeats as novel molecular markers
    A van Belkum
    Department of Medical Microbiology and Infectious Diseases, University Hospital Rotterdam, The Netherlands
    J Clin Microbiol 35:1517-20. 1997
    ..The observed variability in this latter class of VNTRs might be reminiscent of the bacterium's capacity to deal with unfavorable host factors...
  24. pmc On the nature and use of randomly amplified DNA from Staphylococcus aureus
    W Van Leeuwen
    University Hospital Rotterdam Dijkzigt, Department of Bacteriology, The Netherlands
    J Clin Microbiol 34:2770-7. 1996
    ..aureus infections...
  25. ncbi The prevalence and clonal expansion of high-level gentamicin-resistant enterococci isolated from blood cultures in a Dutch university hospital
    N van den Braak
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Antimicrob Chemother 44:795-8. 1999
    ..We conclude that both clonal expansion and the emergence of unique HLGRE have contributed significantly to the increasing incidence of HLGRE...
  26. pmc Molecular characterization of Campylobacter jejuni from patients with Guillain-Barré and Miller Fisher syndromes
    H P Endtz
    Departments of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Clin Microbiol 38:2297-301. 2000
    ..Therefore, further characterization of GBS- or MFS-related C. jejuni should target the genes involved in the synthesis of LPS and the incorporation of sialic acid...
  27. ncbi Induction of antibodies by Staphylococcus aureus nasal colonization in young children
    N J Verkaik
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Microbiol Infect 16:1312-7. 2010
    ..5, p <0.05) were significantly higher in colonized children. The levels of IgA against CHIPS, IsdH and IsdA were higher (p <0.05). Therefore, CHIPS, Efb, IsdA and IsdH seem to play a role in nasal colonization of young children...
  28. pmc Validation of binary typing for Staphylococcus aureus strains
    W Van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Clin Microbiol 37:664-74. 1999
    ..Contribution of the DNA probes to the discriminatory power of the system was analyzed by comparison of dendrograms. This study demonstrates that binary typing is a robust tool for the genetic typing of S. aureus isolates...
  29. ncbi Epidemiology of nasopharyngeal carriage of Neisseria meningitidis in healthy Dutch children
    D Bogaert
    Department of Pediatrics, Sophia Children s Hospital of Erasmus Medical Center, Rotterdam, The Netherlands
    Clin Infect Dis 40:899-902. 2005
    ..The independent determinants of meningococcal carriage included age, regular visits to youth clubs (odds ratio [OR], 2.2) and discotheques (OR, 4.3), and pneumococcal carriage (OR, 4.1)...
  30. doi Streptococcus pneumoniae exposure is associated with human metapneumovirus seroconversion and increased susceptibility to in vitro HMPV infection
    N J Verkaik
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center, Rotterdam, The Netherlands
    Clin Microbiol Infect 17:1840-4. 2011
    ..This was not the case for cells pre-incubated with H. influenzae, M. catarrhalis on S. aureus. We conclude that exposure to S. pneumoniae can modulate HMPV infection...
  31. ncbi Host and pathogen interaction during vaginal infection by Trichomonas vaginalis and Mycoplasma hominis or Ureaplasma urealyticum
    C van der Schee
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam (EMCR, Dr. Molewaterplein 40, 3015 GD, Rotterdam, Netherlands
    J Microbiol Methods 45:61-7. 2001
    ..urealyticum infection. In conclusion, the complex interaction between bacterial and parasitic pathogens and the infected host is determined by genetic characteristics of host and microorganisms involved...
  32. pmc Population studies of methicillin-resistant and -sensitive Staphylococcus aureus strains reveal a lack of variability in the agrD gene, encoding a staphylococcal autoinducer peptide
    W Van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Bacteriol 182:5721-9. 2000
    ..This may suggest that the activity of the agrD gene product does not define colonization dynamics, which is further substantiated by the rarity of agr group 2 and 3 strains...
  33. pmc Identification of Burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods
    C van Pelt
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 37:2158-64. 1999
    ..For final confirmation of species identities, PCR amplification of the small-subunit rRNA gene followed by RFLP analysis with various enzymes is recommended...
  34. doi Course of murine tuberculosis and response to first-line therapy depends on route of infection and inoculum size
    J E M de Steenwinkel
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, University Medical Centre Rotterdam, Rotterdam, The Netherlands
    Int J Tuberc Lung Dis 15:1478-84, i. 2011
    ..In animal models, promising new anti-tuberculosis drugs are assessed in terms of toxic side effects and comparative therapeutic efficacy. Mice are frequently used and experimental infections are established in different ways...
  35. doi High-throughput typing of Staphylococcus aureus by amplified fragment length polymorphism (AFLP) or multi-locus variable number of tandem repeat analysis (MLVA) reveals consistent strain relatedness
    D C Melles
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 28:39-45. 2009
    ..aureus. Despite these dynamic differences, a conservation of type assignments as based upon these two inherently different typing techniques was observed...
  36. pmc Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398
    W J B van Wamel
    ErasmusMC, Department of Medical Microbiology and Infectious Diseases, University Medical Centre Rotterdam, Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 29:119-22. 2010
    ..In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity...
  37. pmc Outbreak of Staphylococcus schleiferi wound infections: strain characterization by randomly amplified polymorphic DNA analysis, PCR ribotyping, conventional ribotyping, and pulsed-field gel electrophoresis
    J Kluytmans
    Department of Clinical Microbiology, Ignatius Hospital Breda, The Netherlands
    J Clin Microbiol 36:2214-9. 1998
    ..This report describes the first well-documented outbreak of S. schleiferi infection. A source of the outbreak was not detected...
  38. doi Immune evasion cluster-positive bacteriophages are highly prevalent among human Staphylococcus aureus strains, but they are not essential in the first stages of nasal colonization
    N J Verkaik
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Microbiol Infect 17:343-8. 2011
    ..011). In conclusion, although IEC-carrying βC-Φs are highly prevalent among human colonizing S. aureus strains, they are not essential in the first stages of S. aureus nasal colonization...
  39. doi High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay
    M A Khan
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Microbiol Infect 16:624-7. 2010
    ..Three Tn1546 variants were found, including a new lineage containing an ISEfa5 insertion in an existing IS1251 element...
  40. pmc Clonal expansion of Staphylococcus epidermidis strains causing Hickman catheter-related infections in a hemato-oncologic department
    J L Nouwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Clin Microbiol 36:2696-702. 1998
    ..These observations underscore the pathogenic potential of clonal CoNS types that have successfully and persistently colonized patients in this hemato-oncology department...
  41. pmc Comparison of eight methods to detect vancomycin resistance in enterococci
    H P Endtz
    Erasmus University Medical Center Rotterdam, The Netherlands
    J Clin Microbiol 36:592-4. 1998
    ..E-test and the agar screen were the only two methods detecting all VRE, including the vanC1/C2 VRE...
  42. ncbi Random amplification of polymorphic DNA versus pulsed field gel electrophoresis of SmaI DNA macrorestriction fragments for typing strains of vancomycin-resistant enterococci
    N van den Braak
    Erasmus University Medical Centre Rotterdam EMCR, Department of MedicalMicrobiology and Infectious Diseases, Rotterdam, The Netherlands
    FEMS Microbiol Lett 192:45-52. 2000
    ....
  43. pmc Proof of principle for successful characterization of methicillin-resistant coagulase-negative staphylococci isolated from skin by use of Raman spectroscopy and pulsed-field gel electrophoresis
    H F M Willemse-Erix
    Center for Optical Diagnostics and Therapy, Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands
    J Clin Microbiol 48:736-40. 2010
    ..It is a tool for screening variability within a collection of isolates. Because of the high throughput, it enables the analysis of multiple colonies per patient, which will enhance the quality of clinical and epidemiological studies...
  44. pmc Density and molecular epidemiology of Aspergillus in air and relationship to outbreaks of Aspergillus infection
    A C Leenders
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center of Rotterdam, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 37:1752-7. 1999
    ..RAPD fingerprinting of Aspergillus isolates can help to determine the cause of an outbreak of invasive aspergillosis...
  45. pmc Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA
    W Van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 39:328-31. 2001
    ..Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories...
  46. ncbi Evaluation of a new commercial immunoassay for rapid detection of Campylobacter jejuni in stool samples
    H P Endtz
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 19:794-7. 2000
    ..The performance of the assay indicates that it has potential value for use in future early intervention studies...
  47. pmc Use of pooled urine samples and automated DNA isolation to achieve improved sensitivity and cost-effectiveness of large-scale testing for Chlamydia trachomatis in pregnant women
    G I J G Rours
    Department of Paediatrics, Erasmus MC Sophia Children s Hospital, Rotterdam, The Netherlands
    J Clin Microbiol 43:4684-90. 2005
    ..Costs per positive case detected in the combined method were 39% of standard costs...
  48. pmc Improved detection of methicillin-resistant Staphylococcus aureus using phenyl mannitol broth containing aztreonam and ceftizoxime
    H Wertheim
    Infectious Diseases, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Clin Microbiol 39:2660-2. 2001
    ..In a clinical setting, 40 MRSA strains were detected with PHMB(+), compared with only 23 detected with a routine method. Thus, this selective broth significantly (P < 0.001) improved the rate of MRSA detection...
  49. pmc Amplified fragment length polymorphism analysis of Campylobacter jejuni strains isolated from chickens and from patients with gastroenteritis or Guillain-Barré or Miller Fisher syndrome
    B Duim
    Department of Bacteriology, Institute for Animal Science and Health, Lelystad, The Netherlands
    Appl Environ Microbiol 66:3917-23. 2000
    ..We conclude that C. jejuni from chickens cannot be distinguished from human strains and that GBS or MFS related strains do not belong to a distinct genetic group...
  50. pmc Methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 in pigs and humans
    Alex van Belkum
    University Medical Center Rotterdam, Rotterdam, The Netherlands
    Emerg Infect Dis 14:479-83. 2008
    ..aureus circulates among humans at low frequency (0.2%) but was isolated in 3 human cases of bacteremia (2.1%; p = 0.026). Although its natural host is probably porcine, ST398 MRSA likely causes infections in humans...
  51. ncbi Complement-resistant Moraxella catarrhalis forms a genetically distinct lineage within the species
    C M Verduin
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    FEMS Microbiol Lett 184:1-8. 2000
    ..All procedures grouped the two variants similarly. Redefinition of the taxonomic status of complement-resistant M. catarrhalis or even the definition of a new species may be opportune...
  52. pmc Heterogeneity of the humoral immune response following Staphylococcus aureus bacteremia
    N J Verkaik
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 29:509-18. 2010
    ..Therefore, vaccines should be based on multiple components. IsdA is immunogenic and, therefore, produced in nearly all bacteremic patients. This suggests that IsdA might be a useful component of a multivalent staphylococcal vaccine...
  53. ncbi Pneumococcal vaccination does not affect the genetic diversity of Moraxella catarrhalis isolates in children
    J P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 23:801-3. 2004
  54. pmc Microbial population diversity in the urethras of healthy males and males suffering from nonchlamydial, nongonococcal urethritis
    W A Riemersma
    Department of Dermatology and Venereology, Erasmus MC, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 41:1977-86. 2003
    ..0079). This suggests that NCNGU might also be diagnosed by assessing the absence rather than the presence of certain bacterial species...
  55. ncbi High-throughput amplification fragment length polymorphism (htAFLP) analysis identifies genetic lineage markers but not complement phenotype-specific markers in Moraxella catarrhalis
    J P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Microbiol Infect 13:55-62. 2007
    ..catarrhalis is probably defined by multiple genes, although not all of these genes may be present within all M. catarrhalis isolates...
  56. pmc External quality assessment of the molecular diagnostics and genotyping of meticillin-resistant Staphylococcus aureus
    R te Witt
    Erasmus MC, Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 29:295-300. 2010
    ..The first molecular typing results are encouraging. Both assessments indicate that programme expansion is required and that major performance discrepancies continue to exist...
  57. ncbi Enterobacter species in a pediatric hospital: horizontal transfer or selection in individual patients?
    P de Man
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands
    J Infect Dis 184:211-4. 2001
    ..A large proportion of the Enterobacter strains was acquired through cross-transmission. This finding contrasts with the prevailing opinion that resistant Enterobacter strains are selected primarily from the patient's own gut flora...
  58. doi Sustained low prevalence of meticillin-resistant Staphylococcus aureus upon admission to hospital in The Netherlands
    L G M Bode
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Centre, Rotterdam, The Netherlands
    J Hosp Infect 79:198-201. 2011
    ..06, Fisher's exact test). This prevalence is still among the lowest in the world, probably as a result of the stringent Dutch infection control policy, and the restrictive use of antibiotics in The Netherlands...
  59. pmc Accuracy of the VITEK 2 system to detect glycopeptide resistance in enterococci
    N van den Braak
    Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Clin Microbiol 39:351-3. 2001
    ..Since the identification and susceptibility data are produced within 3 and 8 h, respectively, VITEK 2 appears a fast and reliable method for detection of GRE in microbiology laboratories...
  60. pmc Long-term cortisol levels are not associated with nasal carriage of Staphylococcus aureus
    L Manenschijn
    Department of Internal Medicine, Erasmus Medical Center, Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 31:97-100. 2012
    ..aureus. Cortisol levels did not differ between these carrier groups (p=0.638). Long-term cortisol levels are not associated with S. aureus nasal carriage...
  61. ncbi Vancomycin resistance: status quo and quo vadis
    H P Endtz
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 18:683-90. 1999
    ..The topics mentioned above are elaborated on and discussed in light of the increasing medical concern on the future detection of microbial infections beyond chemotherapeutic cure...
  62. pmc Lack of association between the presence of the pVir plasmid and bloody diarrhea in Campylobacter jejuni enteritis
    R P L Louwen
    Department Medical Microbiology and Infectious diseases, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    J Clin Microbiol 44:1867-8. 2006
    ..jejuni who developed bloody diarrhea in The Netherlands, and we suggest a role for other virulence determinants...
  63. pmc Follow-up of Staphylococcus aureus nasal carriage after 8 years: redefining the persistent carrier state
    M F VandenBergh
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands
    J Clin Microbiol 37:3133-40. 1999
    ..aureus nasal carriage is a unique characteristic of a fraction of the population, and the attribute "persistent" should be confined to those individuals for whom serial nasal swab specimen cultures consistently yield S. aureus...
  64. pmc Nasal carriage of methicillin-resistant and methicillin-sensitive strains of Staphylococcus sciuri in the Indonesian population
    J A Severin
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, s Gravendijkwal 230, 3015 CE Rotterdam, Netherlands
    Antimicrob Agents Chemother 54:5413-7. 2010
    ..1%), whereas S. sciuri mecA was found in 33 isolates (64.7%). The staphylococcal cassette chromosome mec (SCCmec) regions of S. aureus mecA-positive isolates contained elements of classical S. aureus SCCmec types II and/or III...
  65. ncbi Determinants of Staphylococcus aureus nasal carriage
    J L Nouwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    Neth J Med 59:126-33. 2001
    ..This article aims to review what is currently known of the host and bacterial factors determining S. aureus nasal carriage, including recent developments and future prospects...
  66. doi A novel link between Campylobacter jejuni bacteriophage defence, virulence and Guillain-Barré syndrome
    R Louwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, s Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 32:207-26. 2013
    ..jejuni isolates. Our findings indicate a novel link between viral defence, virulence and GBS in a pathogenic bacterium...
  67. ncbi Dynamics of bacterial colonisation in the respiratory tract of patients with cystic fibrosis
    N Renders
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam (EMCR, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    Infect Genet Evol 1:29-39. 2001
    ..Evolutionary adaptation of pathogens to the CF lung is a common theme in many of these studies...
  68. doi Determinants of Moraxella catarrhalis colonization in healthy Dutch children during the first 14 months of life
    S J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Microbiol Infect 16:992-7. 2010
    ..Apparently, crowding is an important risk factor for early and frequent colonization with M. catarrhalis in the first year of life...
  69. doi Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001-2002
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Microbiology 154:1178-84. 2008
    ..Our results should also be useful in the choice of effective vaccine candidates against M. catarrhalis...
  70. ncbi High density whole genome fingerprinting of methicillin-resistant and -susceptible strains of Staphylococcus aureus in search of phenotype-specific molecular determinants
    Paul H M Savelkoul
    VU University Medical Center, Department of Medical Microbiology and Infection Control, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands
    J Microbiol Methods 71:44-54. 2007
    ..We successfully identify MRSA specific markers and elaborate on the general applicability of the htAFLP approach. This method can be applied to any microbial species for which at least one full-genome sequence is available...
  71. pmc Methicillin-resistant Staphylococcus aureus in meat products, the Netherlands
    Inge H M Van Loo
    St Elisabeth Hospital, Tilburg, The Netherlands
    Emerg Infect Dis 13:1753-5. 2007
    ..We investigated the extent of S. aureus presence in meat and found 36 S. aureus strains in 79 samples. Two strains were MRSA; 1 was multilocus sequence type 398, the clone related to farming...
  72. pmc Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    J Clin Microbiol 45:2698-700. 2007
    ..Samples containing 10(2) to 10(3) MRSA cells were frequently reported to be negative. MRSE samples were scored as negative by all commercial tests but by only two out of three in-house tests...
  73. pmc PCR-restriction fragment length polymorphism analysis of Campylobacter jejuni genes involved in lipooligosaccharide biosynthesis identifies putative molecular markers for Guillain-Barré syndrome
    Peggy C R Godschalk
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Clin Microbiol 45:2316-20. 2007
    ..PCR-restriction fragment length polymorphism analysis of C. jejuni genes involved in the biosynthesis of LOS demonstrated that specific genes were associated with the expression of ganglioside mimics and the development of neuropathy...
  74. ncbi Association between nasal carriage of Staphylococcus aureus and the human complement cascade activator serine protease C1 inhibitor (C1INH) valine vs. methionine polymorphism at amino acid position 480
    Marieke Emonts
    Department of Pediatrics, Erasmus MC Sophia, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 50:330-2. 2007
    ..52, P=0.02). These findings suggest that susceptibility to S. aureus nasal carriage is associated with the C1INH V480M polymorphism...
  75. ncbi The role of human innate immune factors in nasal colonization by Staphylococcus aureus
    Alex van Belkum
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Microbes Infect 9:1471-7. 2007
    ..aureus carriage predisposition. However, MBL haplotypes do so significantly. Baseline HNP1-3 production is more the consequence of S. aureus colonization than a reason for the (non) carrier status...
  76. pmc Comparative genomic analysis of Campylobacter jejuni associated with Guillain-Barré and Miller Fisher syndromes: neuropathogenic and enteritis-associated isolates can share high levels of genomic similarity
    Eduardo N Taboada
    Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, Ontario, K1A 0R6, Canada
    BMC Genomics 8:359. 2007
    ..jejuni factors associated with GBS/MFS to date and has allowed us to analyze the genetic background of neuropathogenic C. jejuni strains with an unprecedented level of resolution...
  77. pmc Unusually high prevalence of panton-valentine leukocidin genes among methicillin-sensitive Staphylococcus aureus strains carried in the Indonesian population
    Juliette A Severin
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, s Gravendijkwal 230, Rotterdam, The Netherlands
    J Clin Microbiol 46:1989-95. 2008
    ..The high prevalence of PVL-positive S. aureus strains in certain regions of Indonesia is of concern since these strains may cause severe infections in the community and in hospitals...
  78. doi Host-microbe interplay in persistent Staphylococcus aureus nasal carriage in HIV patients
    Damian C Melles
    Department of Medical Microbiology and Infectious Diseases, room L 313, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Microbes Infect 10:151-8. 2008
    ..2%) and no PVL positive strains were detected. The population structure of S. aureus strains isolated from HIV patients appeared to be strongly overlapping with that of S. aureus isolates from healthy individuals...
  79. doi Serotyping of Dutch Staphylococcus aureus strains from carriage and infection
    Damian C Melles
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 52:287-92. 2008
    ..Thus, addition of the 336PS conjugate to a type 5- and type 8-CP protein conjugate vaccine would significantly extend the vaccine coverage...
  80. pmc The sialylated lipooligosaccharide outer core in Campylobacter jejuni is an important determinant for epithelial cell invasion
    Rogier Louwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Infect Immun 76:4431-8. 2008
    ..We conclude that sialylation of the LOS outer core contributes significantly to epithelial invasion by C. jejuni and may thus play a role in subsequent postinfectious pathologies...
  81. pmc Key role for clumping factor B in Staphylococcus aureus nasal colonization of humans
    Heiman F L Wertheim
    Erasmus MC, University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    PLoS Med 5:e17. 2008
    ..aureus colonizes the nose are still unknown. The staphylococcal cell-wall protein clumping factor B (ClfB) promotes adhesion to squamous epithelial cells in vitro and might be a physiologically relevant colonization factor...
  82. ncbi Mass spectrometric analysis of intact lipooligosaccharide: direct evidence for O-acetylated sialic acids and discovery of O-linked glycine expressed by Campylobacter jejuni
    Monika Dzieciatkowska
    Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario, Canada
    Biochemistry 46:14704-14. 2007
    ..Our studies demonstrate that MS-based structural analysis of bacterial LOS can be optimized to the level where only a single-colony quantity of material is required and time-consuming chemical treatments can be avoided...
  83. pmc Overlapping population structures of nasal isolates of Staphylococcus aureus from healthy Dutch and American individuals
    Damian C Melles
    Erasmus MC, University Medical Center Rotterdam, room L 313, Department of Medical Microbiology and Infectious Diseases, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    J Clin Microbiol 46:235-41. 2008
    ..In conclusion, the overall population structures of American and Dutch nasal carriage isolates of S. aureus are surprisingly similar, despite subtle geographic differences in the prevalence of certain S. aureus genotypes...
  84. ncbi Nasopharyngeal co-colonization with Staphylococcus aureus and Streptococcus pneumoniae in children is bacterial genotype independent
    Damian C Melles
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Microbiology 153:686-92. 2007
    ..pneumoniae subtypes. Further studies should demonstrate whether differences in bacterial interference are due to more subtle genetic changes...
  85. pmc Moraxella catarrhalis: from emerging to established pathogen
    Cees M Verduin
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, 3015 GD Rotterdam, The Netherlands
    Clin Microbiol Rev 15:125-44. 2002
    ..This review aims to outline our current knowledge of M. catarrhalis, an organism that has evolved from an emerging to a well-established human pathogen...
  86. pmc Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 43:3346-55. 2005
    ..All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time...
  87. pmc Host- and tissue-specific pathogenic traits of Staphylococcus aureus
    Willem B van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Bacteriol 187:4584-91. 2005
    ..Mastitis-associated S. aureus isolated in diverse farm animal species form a distinct genetic cluster, characterized by an overrepresentation of the toxic shock syndrome toxin superantigen-encoding gene...
  88. pmc Loss of the mecA gene during storage of methicillin-resistant Staphylococcus aureus strains
    Arjanne van Griethuysen
    Department of Medical Microbiology and Medical Immunology, Internal Post Number 1630, Hospital Rijnstate Arnhem, P O Box 9025, 6800 EG Arnhem, The Netherlands
    J Clin Microbiol 43:1361-5. 2005
    ..Further analysis of 35 of these isolates confirmed loss of the mecA gene in 32 isolates. This finding has important implications for the management of strain collections...
  89. ncbi Staphylococcus aureus nasal carriage is not associated with known polymorphism in the Vitamin D receptor gene
    Mark Claassen
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 43:173-6. 2005
    ..Our study into genetic determinants of S. aureus carriage patterns is the largest in the field, but still we found no association between VDR gene variation and S. aureus nasal carriage...
  90. pmc Natural population dynamics and expansion of pathogenic clones of Staphylococcus aureus
    Damian C Melles
    University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, room L 313, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Clin Invest 114:1732-40. 2004
    ..We provide evidence that essentially any S. aureus genotype carried by humans can transform into a life-threatening human pathogen but that certain clones are more virulent than others...
  91. ncbi Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers
    Heiman F L Wertheim
    Erasmus MC, University Medical Centre, Rotterdam, Department of Medical Microbiology and Infectious Diseases, Dr Molewaterplein 40, 3015 GD Rotterdam, Netherlands
    Lancet 364:703-5. 2004
    ..006). S aureus nasal carriers and non-carriers differ significantly in risk and outcome of nosocomial S aureus bacteraemia. Genotyping revealed that 80% of strains causing bacteraemia in carriers were endogenous...
  92. ncbi A new high-throughput AFLP approach for identification of new genetic polymorphism in the genome of the clonal microorganism Mycobacterium tuberculosis
    Nicole van den Braak
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    J Microbiol Methods 56:49-62. 2004
    ..tuberculosis. The tests allowed for identification of the major Mycobacterium species and M. tuberculosis variants and clones...
  93. pmc Multilocus sequence typing of Staphylococcus aureus with DNA array technology
    Willem B van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 41:3323-6. 2003
    ..Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant...
  94. pmc Severity of nonbullous Staphylococcus aureus impetigo in children is associated with strains harboring genetic markers for exfoliative toxin B, Panton-Valentine leukocidin, and the multidrug resistance plasmid pSK41
    Sander Koning
    Department of General Practice, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
    J Clin Microbiol 41:3017-21. 2003
    ..The identification of these microbial genetic markers, which are predictive of the severity and the course of the disease, will facilitate guided individualized antimicrobial therapy in the future...
  95. pmc Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spre
    Stephen Murchan
    Laboratory of Hospital Infection, Central Public Health Laboratory, London NW9 5HT, United Kingdom
    J Clin Microbiol 41:1574-85. 2003
    ..This highlights the need for closer international collaboration to monitor the spread of current epidemic strains as well as the emergence of new ones...
  96. ncbi Total genome polymorphism and low frequency of intra-genomic variation in the uspA1 and uspA2 genes of Moraxella catarrhalis in otitis prone and non-prone children up to 2 years of age. Consequences for vaccine design?
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Vaccine 21:1118-24. 2003
    ..Variation in the uspA2 5' non-coding "AGAT" repeat region was also observed. These results may have implications for future M. catarrhalis vaccines comprising UspA1 or UspA2 components...
  97. ncbi Phylogenetic background and virulence profiles of fluoroquinolone-resistant clinical Escherichia coli isolates from the Netherlands
    James R Johnson
    Medical Service, Veterans Affairs Medical Center, Minneapolis, Minnesota, USA
    J Infect Dis 186:1852-6. 2002
    ..These FQREC resemble animal-source E. coli and presumably pose little threat to noncompromised hosts. Similar analysis of other FQREC is needed...
  98. ncbi Intercenter reproducibility of binary typing for Staphylococcus aureus
    Willem B van Leeuwen
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 51:19-28. 2002
    ..The current BT protocol thus represents a simple method generating robust, reproducible genotype data for S. aureus strains...
  99. ncbi Molecular typing of micro-organisms: at the centre of diagnostics, genomics and pathogenesis of infectious diseases?
    Alex van Belkum
    J Med Microbiol 51:7-10. 2002
  100. ncbi Associations between Staphylococcus aureus Genotype, Infection, and In-Hospital Mortality: A Nested Case-Control Study
    Heiman F L Wertheim
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Infect Dis 192:1196-200. 2005
    ..Those infected with S. aureus strains belonging to a CC had higher mortality than those infected with strains not belonging to a CC (P<.05), which indicates the coevolution of S. aureus virulence and spread in humans...
  101. pmc Genotyping of Madurella mycetomatis by selective amplification of restriction fragments (amplified fragment length polymorphism) and subtype correlation with geographical origin and lesion size
    Wendy W J van de Sande
    Erasmus MC University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, The Netherlands
    J Clin Microbiol 43:4349-56. 2005
    ..001). This is the first report on a genotypic study where genetic markers which may be used to study pathogenicity in M. mycetomatis were obtained...