Research Topics
| H G NiestersSummaryAffiliation: Erasmus University Country: The Netherlands Publications
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Detail Information
Publications
Development of a real-time quantitative assay for detection of Epstein-Barr virusH G Niesters
Departments of Virology, University Hospital Rotterdam, Rotterdam, The Netherlands
J Clin Microbiol 38:712-5. 2000..Further studies revealed that the inhibitory effect of heparinized plasma could be efficiently removed by use of an extraction method with Celite as the absorbent...
Molecular and diagnostic clinical virology in real timeH G M Niesters
Department of Virology, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Clin Microbiol Infect 10:5-11. 2004..Finally, with the introduction of a universal internal control throughout the whole procedure, the accuracy of the results generated is warranted...- Clinical virology in real timeHubert G M Niesters
Department of Virology, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
J Clin Virol 25:S3-12. 2002..Since so many targets do have clinical implications, laboratories might prefer to use universal internal controls before the in-house developed assays should be introduced in clinical virology... - Identification of a new variant in the YMDD motif of the hepatitis B virus polymerase gene selected during lamivudine therapyHubert G M Niesters
Department of Virology, University Hospital Rotterdam, The Netherlands
J Med Microbiol 51:695-9. 2002..The mutation was followed by a leucine to methionine change at position 180 (rtL180M). The consequences of this mutation for disease management and diagnostic strategies are discussed... - Quantitation of viral load using real-time amplification techniquesH G Niesters
Department of Virology, University Hospital Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Methods 25:419-29. 2001.... - A multicenter study evaluation of the digene hybrid capture II signal amplification technique for detection of hepatitis B virus DNA in serum samples and testing of EUROHEP standardsH G Niesters
Department of Virology, University Hospital Rotterdam, Rotterdam, The Netherlands
J Clin Microbiol 38:2150-5. 2000..This assay allows the accurate measurement of HBV DNA levels in serum and can be clinically used for the monitoring of responses to antiviral agents for patients chronically infected with HBV... - Identification of more than one mutation in the hepatitis B virus polymerase gene arising during prolonged lamivudine treatmentH G Niesters
Department of Gastroenterology, University Hospital Rotterdam, The Netherlands
J Infect Dis 177:1382-5. 1998..After cessation of therapy, the wild type sequences reemerged... - The nature of acute rejection is associated with development of graft vascular disease after clinical heart transplantationC C Baan
Department of Internal Medicine 1, University Hospital Rotterdam Dijkzigt, Rotterdam, The Netherlands
J Heart Lung Transplant 17:363-73. 1998..To determine mechanisms that trigger graft vascular disease (GVD) after heart transplantation, we studied parameters that reflect both early and late intragraft allogeneic reactions... - Development of a real-time quantitative RT-PCR for the detection of HIV-2 RNA in plasmaM Schutten
Department of Virology, Erasmus Medical Centre Rotterdam, The Netherlands
J Virol Methods 88:81-7. 2000.... - The sequential occurrence of viral mutations in a liver transplant recipient re-infected with hepatitis B: hepatitis B immune globulin escape, famciclovir non-response, followed by lamivudine resistance resulting in graft lossR A de Man
Department of Hepatogastroenterology, Erasmus University Hospital, Rotterdam, The Netherlands
J Hepatol 29:669-75. 1998..The aims were to document the mutations occurring in the hepatitis B virus (HBV) polymerase gene as a consequence of HBIg escape, famciclovir non-response and subsequent lamivudine resistance... - Characteristics of the early phase of chronicity in acute hepatitis B infectionR A Heijtink
Department of Virology, Erasmus University Rotterdam, The Netherlands
J Med Virol 57:331-6. 1999..Differentiation of the antibody response to HBeAg may help to find patients with an increased risk for this interrupted immune clearance who might be candidates for an early intervention therapy... - Clinical utility of viral quantification as a tool for disease monitoringM Schutten
Department of Virology, University Hospital Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Expert Rev Mol Diagn 1:153-62. 2001..New guidelines for disease management, as well as extensive quality control and standardization programs must be introduced... - Nucleoside analogues for chronic hepatitis BL M Wolters
Department of Gastroenterology and Hepatology, University Hospital Rotterdam, The Netherlands
Eur J Gastroenterol Hepatol 13:1499-506. 2001..The best antiviral strategy in the future is probably combination therapy, either with several nucleoside analogues or with a nucleoside analogue and interferon... - Early prediction of response in interferon monotherapy and in interferon-ribavirin combination therapy for chronic hepatitis C: HCV RNA at 4 weeks versus ALTJ T Brouwer
Department of Hepatogastroenterology, Erasmus University Hospital Dijkzigt, Rotterdam, The Netherlands
J Hepatol 30:192-8. 1999..The aim of this study was to assess the predictive value of an HCV RNA test at 4 weeks in comparison with ALT, both in interferon monotherapy and in interferon-ribavirin combination therapy... - Intragraft platelet-derived growth factor-alpha and transforming growth factor-beta1 during the development of accelerated graft vascular disease after clinical heart transplantationH A de Groot-Kruseman
Department of Internal Medicine I, University Hospital Rotterdam Dijkzigt, Rotterdam, The Netherlands
Transpl Immunol 7:201-5. 1999..These results suggest that intragraft PDGF-alpha and TGF-beta1 play a role in the pathogenesis of accelerated GVD after clinical heart transplantation... - Evaluation of a fully automated glycoprotein G-2 based assay for the detection of HSV-2 specific IgG antibodies in serum and plasmaJ Groen
Institute of Virology, Erasmus Medical Center Rotterdam, University Hospital Rotterdam, The Netherlands
J Clin Virol 12:193-200. 1999..Seroepidemiological studies on the prevalence of HSV-2 specific IgG antibodies are especially important to study the impact of this infection among risk groups... - T cells activate the tumor necrosis factor-alpha system during hemodialysis, resulting in tachyphylaxisI C van Riemsdijk
Department of Internal Medicine, University Hospital Rotterdam Dijkzigt, Rotterdam, The Netherlands
Kidney Int 59:883-92. 2001.... - Intragraft interleukin 2 mRNA expression during acute cellular rejection and left ventricular total wall thickness after heart transplantationH A de Groot-Kruseman
Department of Internal Medicine, University Hospital Rotterdam, Rotterdam, Netherlands
Heart 87:363-7. 2002..To assess whether diastolic graft function is influenced by intragraft interleukin 2 (IL-2) messenger RNA (mRNA) expression in rejecting cardiac allografts... - Failure to down-regulate intragraft cytokine mRNA expression shortly after clinical heart transplantation is associated with high incidence of acute rejectionH A de Groot-Kruseman
Department of Internal Medicine, University Hospital Rotterdam Dijkzigt, Rotterdam, The Netherlands
J Heart Lung Transplant 20:503-10. 2001..Therefore, we evaluated intragraft cytokine mRNA expression at an early stage after clinical heart transplantation and related these data to ischemia, immunosuppression, and rejection... - Comparison of reverse hybridization, microarray, and sequence analysis for genotyping hepatitis B virusSuzan D Pas
Erasmus MC, Department of Virology, L355, Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands
J Clin Microbiol 46:1268-73. 2008..3% and a specificity of 100% compared to sequence analysis. HBV DNA-Chip assay showed a sensitivity and specificity of 97.5 and 97.8%, respectively... - Paired measurements of quantitative hepatitis B virus DNA in saliva and serum of chronic hepatitis B patients: implications for saliva as infectious agentAnnemiek A van der Eijk
Department of Hepatology and Gastroenterology, Erasmus MC, University Medical Centre, Room Ca 326, Dr. Molewaterplein 40, 3015 GD Rotterdam, Netherlands
J Clin Virol 29:92-4. 2004..Our findings show that saliva is a source of HBV DNA.This finding may have implications in selected patients for the infectivity of saliva and offer further insight in the routes of transmission of HBV infection... - Functional impairment of myeloid and plasmacytoid dendritic cells of patients with chronic hepatitis BRenate G van der Molen
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
Hepatology 40:738-46. 2004..In conclusion, mDC and pDC are functionally impaired in patients with chronic hepatitis B. This might be an important way by which hepatitis B virus evades an adequate immune response, leading to viral persistence and disease chronicity... - Pegylated interferon alfa-2b alone or in combination with lamivudine for HBeAg-positive chronic hepatitis B: a randomised trialHarry L A Janssen
Department of Gastroenterology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands
Lancet 365:123-9. 2005..Treatment of HBeAg-positive patients with chronic hepatitis B is not effective in most. A combination of immunomodulatory pegylated interferon alfa-2b and antiviral lamivudine might improve the rate of sustained response... - Quantification of the newly detected lamivudine resistant YSDD variants of Hepatitis B virus using molecular beaconsSuzan D Pas
Department of Virology, ErasmusMC, University Medical Center Rotterdam, Rotterdam, The Netherlands
J Clin Virol 32:166-72. 2005..With this technique, new information on the dynamics of variants during and after therapy was obtained... - Paired, quantitative measurements of hepatitis B virus DNA in saliva, urine and serum of chronic hepatitis B patientsAnnemiek A van der Eijk
Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Centre Rotterdam, The Netherlands
Eur J Gastroenterol Hepatol 17:1173-9. 2005..The potential infectivity of these body fluids may provide an explanation for the 20% of cases of infection obtained through horizontal transmission for which the origin of infection is yet unknown... - The effect of pegylated interferon-alpha on the treatment of lamivudine resistant chronic HBeAg positive hepatitis B virus infectionWilhelmus F Leemans
Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands
J Hepatol 44:507-11. 2006..In our opinion, an analysis of the patient subgroup harbouring an YMDD-mutation should be included in all future studies of pegylated interferon-alpha in chronic hepatitis B... - Analysis of intrahepatic HBV-specific cytotoxic T-cells during and after acute HBV infection in humansDave Sprengers
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
J Hepatol 45:182-9. 2006..Their presence in the liver may suggest a role in the resolution of the infection. Intrahepatic HBV-specific CD8 + T cells remain detectable at high frequencies after HBsAg-seroconversion... - Cowpox virus transmission from rats to monkeys, the NetherlandsByron E E Martina
Erasmus Medical Center Rotterdam, Rotterdam, Netherlands
Emerg Infect Dis 12:1005-7. 2006..Sequence comparison and serologic results showed that brown rats (Rattus norvegicus) transmitted the virus to monkeys... - The dynamics of mutations in the YMDD motif of the hepatitis B virus polymerase gene during and after lamivudine treatment as determined by reverse hybridisationSuzan D Pas
Department of Virology, University Hospital Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
J Clin Virol 25:63-71. 2002..In three patients, no variant virus population could be detected at 25, 36 and 37 weeks, respectively, after cessation of treatment. This observation is important for the inclusion of the so-called naive patients in clinical trials... - Viral dynamics in chronic hepatitis B patients during lamivudine therapyLonieke M M Wolters
Department of Hepatogastroenterology, University Hospital Rotterdam, The Netherlands
Liver 22:121-6. 2002..The bi-phasic model as described by Neumann et al. in combination with frequent blood sampling provides the most accurate fit and can be used to compare new nucleoside analogue profiles to lamivudine therapy... - Detection of HBV DNA using real time analysisSuzan D Pas
Department of Virology, Erasmus Medical Center, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
J Clin Virol 25:93-4. 2002 - The influence of baseline characteristics on viral dynamic parameters in chronic hepatitis B patients treated with lamivudineLeonieke M M Wolters
Department of Hepatogastroenterology, University Hospital Rotterdam, The Netherlands
J Hepatol 37:253-8. 2002..This strengthens the hypothesis that the level of intrinsic activity is related to the turnover of infected hepatocytes. Moreover, reintroduction of lamivudine in previously lamivudine-treated patients should be considered carefully... - Viral dynamics during and after entecavir therapy in patients with chronic hepatitis BLeonieke M M Wolters
Department of Gastroenterology and Hepatology, University Hospital Rotterdam, Rotterdam, The Netherlands
J Hepatol 37:137-44. 2002..Both can be described with a mathematical model. Data on levels of cccDNA in the liver in these patients could be helpful in supporting the parameters as calculated with the model... - Absolute level of Epstein-Barr virus DNA in human immunodeficiency virus type 1 infection is not predictive of AIDS-related non-Hodgkin lymphomaDebbie van Baarle
Department of Clinical Viro Immunology, Sanquin Research at CLB, and Department of Hematology, Academic Medical Center, University of Amsterdam, 1066 CX Amsterdam, The Netherlands
J Infect Dis 186:405-9. 2002..EBV DNA was also detectable in serum at some time points but at a lower level... - Persistence of YMDD variants after withdrawal of LamivudineAnnemiek A van der Eijk
J Hepatol 36:304-5. 2002 - Viral dynamics in chronic hepatitis B patients treated with lamivudine, lamivudine-famciclovir or lamivudine-ganciclovirLeonieke M M Wolters
Department of Gastroenterology and Hepatology, University Hospital Rotterdam, 3000 CA Rotterdam, The Netherlands
Eur J Gastroenterol Hepatol 14:1007-11. 2002..Different combinations of nucleoside analogues need to be studied in order to obtain a major breakthrough in this treatment strategy... - Molecular epidemiology of gibbon hepatitis B virus transmissionSuwanna Noppornpanth
Viral Hepatitis Research Unit, Department of Paediatrics, Chulalongkorn University and Hospital, Bangkok 10330, Thailand
J Gen Virol 84:147-55. 2003..Although some of the animal caretakers at the Krabok Koo Wildlife Breeding Centre were found to be chronic HBV carriers, genotype and sequence analysis did not reveal any evidence for zoonotic disease transmission... - Human bocavirus and acute wheezing in childrenTobias Allander
Department of Microbiology Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
Clin Infect Dis 44:904-10. 2007..It has been detected primarily in children with acute lower respiratory tract infection, but its occurrence, clinical profile, and role as a causative agent of respiratory tract disease are not clear... - Clinical, immunological and virological response to different antiretroviral regimens in a cohort of HIV-2-infected patientsMarchina E van der Ende
University Hospital Rotterdam, Dijkzigt, Rotterdam, The Netherlands
AIDS 17:S55-61. 2003..To assess the clinical, immunological and virological response and the emergence of resistance towards antiretroviral therapy (ART) in a cohort of HIV-2-infected patients... - Respiratory viruses and acute asthma in childrenTuomas Jartti
J Allergy Clin Immunol 120:216; author reply 217. 2007 - Accuracy of a referral guideline for patients with chronic hepatitis B in primary care to select patients eligible for evaluation by a specialistIrene K Veldhuijzen
Gut 56:1027-8. 2007 - Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureusAlex van Belkum
Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
J Clin Microbiol 45:2698-700. 2007..Samples containing 10(2) to 10(3) MRSA cells were frequently reported to be negative. MRSE samples were scored as negative by all commercial tests but by only two out of three in-house tests... - Impact of human bocavirus on children and their familiesSusanna Esposito
Institute of Pediatrics, University of Milan, Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Via Commenda 9, 20122 Milano, Italy
J Clin Microbiol 46:1337-42. 2008.... - Presence or absence of cytomegalovirus in cerebrospinal fluid from patients with Guillain-Barré syndrome?Mark L Kuijf
J Infect Dis 193:1471-2; author reply 1472-3. 2006 - Prevention of Epstein-Barr virus-lymphoproliferative disease by molecular monitoring and preemptive rituximab in high-risk patients after allogeneic stem cell transplantationJoost W J van Esser
Department of Hematology, Erasmus Medical Center/Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
Blood 99:4364-9. 2002..04) at 6 months from EBV-DNA more than or equal to 1000 geq/mL. Frequent quantitative monitoring of EBV reactivation and preemptive therapy by rituximab improves outcome in patients at high risk of EBV-LPD. (Blood. 2002;99:4364-4369).. - Detection of hepatitis B virus genotypes and mutants: current statusHubert G M Niesters
Department of Virology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
J Clin Virol 34:S4-8. 2005..Technologies enabling the detection and quantification of these variant populations are presented and discussed... - Characterization of Epstein-Barr virus Type I variants based on linked polymorphism among EBNA3A, -3B, and -3C genesIrene Görzer
Medical University of Vienna, Institute of Virology, Kinderspitalgasse 15, A 1095 Vienna, Austria
Virus Res 118:105-14. 2006..Thus, a combination of more than one polymorphic site in the EBV genome might be involved in determining disease characteristics... - Real-time Epstein-Barr virus PCR for the diagnosis of primary EBV infections and EBV reactivationRianne Luderer
Unit Molecular Diagnostics, Diakonessenhuis Utrecht, Utrecht, The Netherlands
Mol Diagn 9:195-200. 2005..Recently, EBV PCR has been added as a diagnostic tool. Positive EBV PCR has been demonstrated in the serum of patients with primary EBV infections and EBV reactivation... - Antiviral treatment is more effective than smallpox vaccination upon lethal monkeypox virus infectionKoert J Stittelaar
Department of Virology, Erasmus MC, 3000 DR Rotterdam, The Netherlands
Nature 439:745-8. 2006..These data show that adequate preparedness for a biological threat involving smallpox should include the possibility of treating exposed individuals with antiviral compounds such as cidofovir or other selective anti-poxvirus drugs... - Acquisition and clearance of perianal human papillomavirus infection in relation to HIV-positivity in men who have sex with men in the NetherlandsEric M van der Snoek
Department of Dermatology and Venereology, Institute of Epidemiology and Biostatistics, Erasmus MC, Rotterdam, The Netherlands
Acta Derm Venereol 85:437-43. 2005..036) while the incidence of type 16 may be associated with HIV positivity (p=0.059). In HIV-positive MSM significantly more high-risk HPV types were detected at the perianal region... - Functional heme oxygenase-1 promoter polymorphism in relation to heart failure and cardiac transplantationCecile T J Holweg
Department of Internal Medicine, University Medical Center Rotterdam, Rotterdam, The Netherlands
J Heart Lung Transplant 24:493-7. 2005..No influence of the polymorphism on the development of heart failure, graft survival, acute rejection, or transplant atherosclerosis was found... - Standardisation and controls, why can't we overcome the hurdles?Hubert G M Niesters
J Clin Virol 31:81-3. 2004 - Polyarteritis nodosa associated with hepatitis B virus infection. The role of antiviral treatment and mutations in the hepatitis B virus genomeHarry L A Janssen
Department of Gastroenterology, Erasmus MC, University Medical Centre, and Virology, Erasmus University, Rotterdam, The Netherlands
Eur J Gastroenterol Hepatol 16:801-7. 2004..The efficacy of new generation nucleoside analogues should be further elucidated in future studies... 
Association between high nasopharyngeal viral load and disease severity in children with human metapneumovirus infectionSamantha Bosis
Institute of Pediatrics, University of Milan, Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Via Commenda 9, 20122 Milan, Italy
J Clin Virol 42:286-90. 2008..Previous studies have shown that viral genotype and viral load may play a significant role in the pathogenesis of viral infections...